684 results about "Pollination" patented technology

A Chinese cabbage hybrid seed production marking method relates to the technical field of Chinese cabbage hybrid seed production. The technical scheme of the method is as follows: after Chinese cabbage hybrid seed production pollination is finished, firstly holding the pedicel of a pollinated flower with the left hand; then transversely tearing off two sepals from the root with the right hand, wherein light movement is required. The Chinese cabbage hybrid seed production marking method can perform marking without rope tying, ring arranging or stamp-pad ink coating, and is low in cost and high in accuracy.

The invention discloses an acer elegans cross breeding method based on flowering phase difference regulation and control, and belongs to the technical field of garden plant breeding. The method comprises the following steps: selecting an acer elegans variety of which the natural early flowering phase difference is greater than or equal to 10 days and the genetic distance is greater than or equal to 0.5 as a cross parent, carrying out low-temperature refrigeration and shading blue light supplementing treatment on a female parent to delay the flowering phase, and carrying out prolonged illumination, red light supplementing and hormone and nutrient solution spraying on a male parent to advance the flowering phase; male parent pollen is collected through a vacuum negative pressure adsorption device and stored in a closed container, a sucrose solution is sprayed to female parent stigmas before pollination, and repeated pollination is conducted through a directional nozzle at 9-11 o'clock every day; carrying out stratification treatment and temperature-difference temperature-control germination on the hybrid seeds, and carrying out light-temperature regulation and control seedling culture after sowing; the molecular marker is used for verifying the hybridization authenticity and target traits, phenotype determination is carried out on three-year-old seedlings to complete breeding, the hybridization obstacle caused by asynchronous flowering phases of the caenorhabditis elegans varieties is solved through the flowering phase regulation and control technology, and the hybridization success rate is increased.

The present invention relates to a polypeptide CYS3 that affects seed germination. The CYS3 polypeptide molecule affects the germination of immature tobacco seeds. Immature tobacco seeds picked 20 days in advance after pollination are used as test materials. The CYS3 polypeptide is expressed and then treated in the tobacco seeds. It is found that the CYS3 protein has a germination-promoting effect on the immature tobacco seeds, providing a practical technology for promoting tobacco seed germination and seedling emergence in production.

The invention belongs to the technical field of plant biology, and particularly relates to a genetic transformation method based on oat immature embryos, which comprises the following steps: preparing an infection solution containing a target gene; taking immature caryopses of the oats 21-23 days after pollination; infecting the immature embryos with infection liquid, and co-culturing the immature embryos after infection is completed; after co-culture is completed, immature embryos are sequentially transferred into an L3-T culture medium and an L3-B5 culture medium to induce formation of calluses, and after the calluses are formed, the calluses are sequentially induced to be differentiated, rooted and hardened. According to the method, efficient genetic transformation of exogenous genes is realized by selecting proper oat immature embryos, proper culture conditions and proper transformation conditions. By means of the genetic transformation method, the genetic transformation efficiency of the oats is improved to 25% from 2%-3%. And meanwhile, the immature embryo is used as a transformation acceptor material, and the complete plant can be efficiently and stably regenerated by the method disclosed by the invention.

The invention provides a bumblebee attractant and a method for pollinating camellia oleifera by utilizing bumblebees, and belongs to the technical field of camellia oleifera pollination. The bumblebee attractant comprises the following components: eugenol, methyl jasmonate, glucose, a solubilizer and a diluent. The method for pollinating the camellia oleifera by using the bumblebees comprises the following steps: S1, 1-2 weeks before pollination, performing disinfection and insecticidal treatment on a camellia oleifera tree entering row; s2, 2-3 days before flowering of camellia oleifera, bumblebee beehives are placed in a camellia oleifera garden, and hydromel is fed; s3, when male flowers and female flowers of camellia oleifera bloom and the weather is sunny, spraying the bumblebee attractant to stigmas of the female flowers every day, and meanwhile, releasing pollinated bumblebees from the beehive until the flowering phase of the female flowers ends. Eugenol and methyl jasmonate in the attractant can synergistically improve the attraction effect on bumblebees, and the pollination rate of camellia oleifera is increased.

The invention relates to the field of hybrid rice breeding, and discloses a method for directionally breeding mutant hybrid rice parents. According to the method, mutation tillering is accurately positioned in the M1 tillering stage through the KASP technology, and a directional pollination / harvesting strategy is combined, so that the genetic bottleneck caused by chimeras and low fertility (especially sterile lines) is effectively overcome, the mutation transmission efficiency is maximized, and the M1 generation mutation transmission efficiency is remarkably improved. Through early accurate identification and efficient propagation of the M1 generation, homozygotes can be obtained and screened from the M2 generation, and compared with a traditional process, the method has the advantages that the obtaining process of low-cadmium homozygous parents is obviously accelerated, and the breeding period is greatly shortened. Heavy ion mutagenesis, targeted sequencing, high-throughput KASP typing and directional propagation technologies are integrated, and the whole process from mutagenesis to obtaining of the homozygous low-cadmium parent is rapid, accurate and efficient.

The utility model relates to the technical field of plant pollen collection, and discloses a rapid field apocynum venetum pollen collecting device which comprises a pollen scattering device, the pollen scattering device comprises a stamen inlet, a stamen processing pipe is welded to the bottom of the stamen inlet, a pollen scattering and spreading cavity is welded to the bottom of the stamen processing pipe, and the pollen scattering and spreading cavity is communicated with the stamen inlet. Four cavity wall attachment connecting strips are welded to the inner side of the pollen scattering and spreading cavity, a middle rotating shaft is welded to one ends of the four cavity wall attachment connecting strips, a wind driven fan is connected to the inner side of the middle rotating shaft through a rotating shaft, and a pollen scattering brush is welded to the top of the wind driven fan; an impurity filter screen is connected to the middle of the inner side of the pollen scattering and spreading cavity through a bolt, and the problems that according to an existing rapid field apocynum venetum pollen collecting device, pollen falls off through mechanical vibration or airflow blowing, and certain influences are caused to plants in actual use, such as flower bud damage and pollination influence are solved.

The invention discloses an assembly sequence planning method based on a multi-strategy improved flower pollination algorithm, and relates to the research field of assembly sequence planning. According to the method, on the basis of assembly body information, the assembly direction transformation cost, the assembly tool transformation cost and the assembly operation difficulty cost are set as assembly cost evaluation indexes of the assembly sequence, and a sequence evaluation system is constructed through quantitative evaluation of the constructed assembly sequence cost. The improved flower pollination algorithm based on multiple strategies is provided, the convergence efficiency of assembly sequence planning optimization can be improved, the method has more advantages than a single algorithm, the problem that sequence optimization falls into a local optimal solution can be solved, and a new thought and an efficient method are provided for assembly planning research. The method aims at solving the problems that in industrial product assembly sequence planning, a flower pollination algorithm is weak in solution capacity in a discrete spatial domain, prone to falling into local optimum, low in solution efficiency and the like, the optimal assembly sequence is rapidly solved, then the production time and cost of industrial products are reduced, and the assembly quality is improved.

The invention discloses a special feed for breeding bumblebee pollination swarms and a preparation method thereof, and belongs to the technical field of insect feed preparation, and the special feed for breeding the bumblebee pollination swarms comprises the following raw materials: white granulated sugar, glucose, potassium sorbate, epsilon-polylysine, vitamins, trace elements and water; the preparation method comprises the following steps: adding the white granulated sugar and the glucose into boiling water, uniformly stirring, cooling, adding the vitamins, the trace elements, the potassium sorbate and the epsilon-polylysine, uniformly stirring, and cooling to room temperature. The components such as the sugar source, the vitamins and the preservative are introduced to achieve a synergistic effect, so that the freshness of the sugar water is guaranteed, the intake of nutrient substances by the bee colony is improved, the survival rate of the bee colony is effectively increased, the development time of the early-stage bear bee colony is shortened, the growth speed of the early-stage pollination bee colony is accelerated, and the colony potential of the bee colony before pollination is increased.

The utility model provides an artificial pollination tool for breeding, which comprises a box body, a pollen storage box with a conical bottom is arranged on the box body, a discharge pipe is communicated with the bottom of the pollen storage box, a rotating shaft is arranged in the pollen storage box and the discharge pipe, an auger blade is arranged on the rotating shaft, a pipeline is arranged at the box body in a penetrating manner, and the auger blade is arranged on the pipeline. The pipeline is fixedly connected with the box body, the pipeline is communicated with the discharging pipe, an air pump is installed in the box body, an air outlet of the air pump is communicated with the pipeline through a hose, the part, located outside the box body, of the pipeline is sleeved with an adjusting pipe, and the adjusting pipe is in sliding connection with the pipeline. According to the artificial pollination device, the problems that the existing artificial pollination mostly adopts manual pollination, the operation is inconvenient, the coverage area of pollination is small, the pollen coverage is non-uniform, and the labor cost is relatively high are solved.

A pollination control structure comprises a support; a plurality of support fixings, each support fixing being attached to or integral with the support, and; a barrier member comprising a plurality of barrier member pieces. At least one of the barrier member pieces is releasably attached to at least one of the support fixings by an intermediate fixing. The barrier member piece is releasably attached to the intermediate fixing, and the intermediate fixing is releasably attached to the support fixing.

The embodiment of the invention provides a path generation method and device of an unmanned aerial vehicle, the unmanned aerial vehicle and a storage medium. Relates to the technical field of unmanned aerial vehicles. The method comprises the following steps: acquiring a path generation request; wherein the path generation request comprises a starting point and an ending point. And based on the path generation request, performing path optimization processing on a path between the starting point and the ending point according to a preset flower pollination algorithm to generate an initial path. And obtaining path sample data according to a preset reinforcement learning algorithm, and performing path iteration processing on the path sample data and the initial path to generate a target path. The method is used for achieving the effect of improving the accuracy of path planning and emergency risk avoiding of the unmanned aerial vehicle.

The embodiments of the present specification disclose a path planning method, device and medium for flower pollination, which relate to the field of flower pollination technology. The method includes: collecting point cloud data of plants to be pollinated for semantic segmentation to generate style position data; determining style posture data based on the style position data through a point pair feature method, and determining the stamen orientation information of each style to be pollinated; determining the pollination path planning end point and a pre-pollination point based on the stamen orientation information, calculating the distance between the pre-pollination point and the pollination path planning end point, and when the distance is greater than a preset distance setting value, constructing a reference coordinate system through the pre-pollination point and the pollination path planning end point; determining a leaf obstacle set in the reference coordinate system, and establishing a sampling space based on the leaf obstacle set in a specified area, so as to generate a pollination path corresponding to each style to be pollinated in the established sampling space through a BI-RRT algorithm of an improved artificial potential field method, thereby realizing pollination of the plants to be pollinated.

The invention discloses a deep learning-based collapsibility grade automatic discrimination method. The method comprises the following steps of S1, generating a preprocessed loess geological engineering index data set; s2, obtaining a loess sampling unit graph structure data set with a weighted adjacent matrix; s3, forming a loess sampling unit weighted graph structure data set with node attributes; s4, constructing a multi-target comprehensive fitness function; s5, randomly generating differential flower pollination algorithm population individuals, and performing global search and local search on the differential flower pollination algorithm initial population to obtain an optimal graph attention network hyper-parameter vector and an optimal node feature selection mask vector; and S6, obtaining an optimized graph attention network model, deducing the loess sampling unit weighted graph structure data set with node attributes, and outputting a collapsibility grade prediction result of each sampling unit. According to the method, the influence of misjudgment of different collapsibility levels on engineering safety is dynamically evaluated, so that the algorithm is guided to preferentially improve the recognition accuracy of high-risk levels.

According to the method, the pollination problem of an endangered plant, namely the cymbidium serotina, is researched, and in order to improve the pollination rate of the cymbidium serotina and optimally select the pollen collection time, the pollination tool and the pollination time, the invention provides a method for adopting cactus thorns as the pollination tool for the first time, so that the pollination of the cymbidium serotina is facilitated, and the pollination efficiency of the cymbidium serotina is improved. The pollen utilization rate is greatly improved, the breeding number of the precious relict plants is increased, and an efficient pollination method is provided for breeding of the rare and endangered plants.

The invention relates to an attractant for efficient pollination of muskmelons and a preparation method thereof, and belongs to the technical field of crop bee pollination, the attractant comprises the following raw materials: 15-20 parts of a modified microcapsule compound, 10-15 parts of a saccharide active component, 4-6 parts of a solubilizer, 3-5 parts of an emulsifier and 60-70 parts of water; according to the technical scheme, hydroxypropyl-beta-cyclodextrin and Schiff alkalized mesoporous silica are combined to obtain the composite material; the composite material is firstly mixed with an essential oil compound and then mixed with a color active component, and a functional material is obtained; the preparation method comprises the following steps: coating a functional material with Arabic gum and polycaprolactone to obtain a modified microcapsule compound; and mixing the modified microcapsule compound, a solubilizer, an emulsifier, a saccharide active component and water to finally prepare the attractant for efficient pollination of the muskmelon. The slow release performance, the attraction performance and the stability of the attractant are integrally improved, the shelf life of the attractant is prolonged, the pollination efficiency of muskmelon flowers is improved, and the comprehensive performance is good.

The invention discloses a method for improving the quality of epimedium seeds and application thereof, and the method comprises the following steps: S1, carrying out fixed-plant and fixed-point observation in a homogeneous garden, recording and observing the flowering habit and synchronism of epimedium delavayi, including the flowering period time and pollination insect species, and observing the flower characteristics; stigma pollination is determined through pollen activity detection, and a basis is provided for subsequent artificial isolated pollination; s2, performing artificial isolation pollination in the flowering phase of the epimedium villosum plants, and using an isolation closing device to prevent cross-pollination. According to the method for improving the quality of the herba epimedii seeds and the application of the method, the flowering rule of the herba epimedii is determined through homogeneous garden transplanting experiments, the pollen viability is dynamically monitored in combination with a TTC staining method, an artificial isolation pollination device and an operation process are designed, and the quality of the herba epimedii seeds is improved. The method has the following technical effects that the seed purity is improved, wherein the seed purity after artificial isolation pollination reaches 90% or above, and the genetic background consistency is remarkably improved.

The invention discloses a ZaSCL3 gene for regulating and controlling plant growth and development as well as an encoding protein and application thereof, and belongs to the technical field of gene engineering. According to the invention, an over-expression vector of the ZaSCL3 gene is constructed and expressed in a wild tomato, so that stamens of a ZaSCL3 over-expression plant are aborted, ovary is spontaneously expanded without pollination, and seedless fruits are generated, which indicates that the over-expression ZaSCL3 gene can realize the effect of inducing male sterility and apomixis of the plant; a key gene resource is provided for molecular breeding and yield character improvement, and the important application value is achieved.

The invention belongs to the field of biological prevention and control, and discloses an insect specific virus NLBV3 and application thereof in reducing transmission of rice odontodwarf virus of brown planthopper. The NLBV3 virus genome comprises three chains, the L chain encodes RNA dependent RNA polymerase (RdRP), the M chain encodes glycoprotein precursor (GP), the S chain encodes nucleoprotein (NP), and the nucleotide sequences are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3; the application is realized by releasing the NLBV3-carrying brown planthopper population, and according to the screening and detection method of the NLBV3-carrying brown planthopper high-virus population provided by the invention, natural brown planthoppers can be effectively infected after the NLBV3-carrying brown planthopper high-virus population is released, the RRSV transmission capability of the brown planthoppers is reduced, and the virus is prevented from damaging rice production. The NLBV3 provided by the invention is an insect specific virus, is harmless to mammals, fishes and shrimps, natural enemy insects and pollination insects, and has the advantages of specific action object, environment friendliness and the like.

The invention belongs to the technical field of biology, and particularly relates to an Indel molecular marker related to a corn plant height character and application of the Indel molecular marker. The plant height of a corn plant containing the Indel molecular marker is higher than that of a corn plant without the Indel molecular marker. An effective and reliable molecular marker is provided for identification of the plant height character of corn and prediction of a screening machine, the molecular marker is applied to improved inheritance and breeding of the plant height character of corn, the breeding period can be effectively shortened, the breeding process is accelerated, the breeding efficiency is improved, and the process of screening excellent germplasm resources is accelerated; a new direction is provided for reducing the breeding cost, for example, pollination and breed conservation can be carried out on a corn single plant without the InDel molecular marker, and corn seed production can be carried out. The invention provides a new direction for screening, identifying, breeding and the like of the dwarf corn through a molecular assisted selection means.

The invention discloses a plant gene function analysis method (FAGEN), and belongs to the technical field of molecular genetics and functional genomics. The method comprises the following steps: constructing a target gene mutant by using a gene editing technology; hybridizing the mutant and the corresponding wild type as a common parent with a plurality of parents with different genetic backgrounds to construct a pair F1 group; an F2 group is obtained through selfing; and determining phenotypic data of target characters of F1 and F2 populations, and comparing phenotypic differences of filial generations of wild types and mutants under different genetic backgrounds to judge the functions of the target genes. The method has the advantages of simplicity in operation, flexibility in design, short construction period and low cost, and gene functions can be efficiently and accurately analyzed under various genetic backgrounds.

The invention discloses a selenium-rich foliar fertilizer for pitaya and a preparation method thereof, and belongs to the technical field of pitaya planting.By means of the selenium-rich foliar fertilizer for the pitaya, common pitaya can be converted into selenium-rich functional fruits with high added value, and the selenium-rich foliar fertilizer is accurately applied in key periods of 3 days, 13 days and 25 days after flowering and pollination, so that the yield of the pitaya is increased, and the yield of the pitaya is increased. The selenium element accumulation and fruit weight gain effects are maximized, and meanwhile, the selenium element is quickly transferred to the fruits after being absorbed by leaves by targeted spraying on the fruits and branches, so that the bioavailability of selenium is improved; the foliage application mode avoids excessive accumulation of selenium in soil, prevents the risk of soil selenium pollution, promotes the development of ecological agriculture, solves the problem of lack of special selenium-rich fertilizer for pitaya compared with the prior art, realizes synchronous improvement of nutritional quality and yield, and is suitable for large-scale popularization and application. The key technical support is provided for the development of the high-quality functional dragon fruit industry.

The artificial breeding and island plant pollination method comprises the following steps: artificial breeding of aphids: broad bean seedlings are planted in an insect breeding cage to propagate black bean aphids, so that adults of the hoverflies can lay fly eggs, and larvae of the hoverflies can eat the fly eggs at the same time; feeding of hoverfly larvae: feeding the hoverfly larvae with black bean aphids in an insect breeding cage, and enabling the hoverfly larvae to develop into pupae after 6 days; collecting pupae of hoverflies with short thorn legs: harvesting broad bean seedlings with mature larvae of hoverflies, putting the broad bean seedlings into a plastic box with a gauze opening for continuous feeding, and putting the collected pupae of hoverflies into a culture dish until all the larvae in the plastic box pupate; and transporting and releasing the short-spur hoverflies, namely storing pupae of the hoverflies in a pupa card device at the temperature of 4 DEG C, transporting the pupae to an open sea island, and releasing the pupae. By the adoption of the method, scale breeding can be conducted on the species of the hoverfly brachypoda, and the hoverfly brachypoda can be transported to the island area and used for pollination of island plants.

The invention discloses a method for accurately identifying sweet-waxy corn germplasm, and particularly relates to a method for identifying sweet-waxy corn germplasm, which comprises a first tracking process: a process from F3-generation seed budding to F4-generation seed maturation, including sweet-waxy gene DNA tracking marking after seed budding; sampling once 28 days after pollination, and measuring the thickness of soluble sugar, amylose, amylopectin and pericarp; extracting DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) of F3-generation plants in a After the F4-generation seeds are mature and picked, determining sweet-waxy gene DNA, determining amylose and amylopectin, and determining single-plant grain weight, hundred-grain weight and endosperm / embryo dry weight; a second tracking process: a process from germination of the F4-generation seeds to bearing of fresh sweet-waxy corn, including observation and recording of a field corn plant growth process; measuring the thickness of soluble sugar, amylopectin, amylose and pericarp 15 days after pollination; determining and analyzing the traits of the indoor test species; data analysis and germplasm scoring: comparing and analyzing the data obtained in the second tracking process, scoring the F4-generation seeds, and determining the germplasm quality of the F4-generation seeds.

The invention belongs to the field of avocado cultivation, and discloses an efficient cultivation method for reducing avocado root rot, which comprises the following steps: planting 3-5 different varieties of avocado seedlings together according to the plant spacing of 1-1.5 m; a high-quality material is used as a main cultivar, other plants are pollinizers, and trunks can be cut when the plants grow to 60 cm; when lateral branches intersect, high-altitude grafting can be conducted, and binding type cultivation is conducted after healing. According to the method, a multi-tree binding high-position grafting technology is adopted, each tree can be reinforced, typhoon prevention and root rot reduction are achieved, the proportion of varieties in binding is well matched, the pollination and fertilization probability is improved, and the purposes of high yield and stable yield are achieved.

The invention relates to the field of tomato pollination, and provides a tomato adaptive pollination method, device and equipment, a storage medium and a program product, and the method comprises the following steps: obtaining a three-primary color image set and a three-dimensional point cloud picture of a tomato plant; training a target detection model through the three-primary color image set to obtain a flowering period segmented image output by the target detection model; training a deep learning network through the segmented image of the flowering period and a fused image of the three-dimensional point cloud image to obtain a neural network model; and controlling a target robot to pollinate the tomato plant based on the flower posture and the flower space coordinate output by the neural network model. According to the invention, the flowering stage flowers are identified through the target detection model, the flowering stage flowers are positioned through the neural network model, and the tomato flowers are adaptively pollinated through the target robot based on the postures and positions of the flowers, so that the pollination efficiency and accuracy are improved.

The invention relates to the technical field of plant planting, in particular to a method for promoting flowering and fruit setting of amomum tsao-ko and application thereof.In the vigorous vegetative growth period, a nutrient solution composed of ammonium sulfate, NAA and GA3 with the proper concentration is adopted, the vigorous vegetative growth period can be effectively prolonged, and therefore flower bud differentiation is inhibited; a nutrient solution B composed of monopotassium phosphate, ABA and BR is sprayed in April, vegetative growth can be effectively broken through to be converted into reproductive growth, flower buds are promoted to be rapidly differentiated and formed when a rainy season comes, the flowering rate can be increased by 28-33%, the yield of the amomum tsao-ko medicinal material can be greatly increased, bumblebees are quantitatively put in the early stage of May, and the pollination rate can be greatly increased; in the blooming period, the nutrient D is spread around the cluster buds, meanwhile, the nutrient solution E is sprayed in a matched mode, and the fruit setting rate can be increased by 8.9%-9.6% compared with the fruit setting rate.

Automated precision applicator for row crops with dual sided applicators that can be used to direct pollen to multiple areas of the plant, as well as to measure phenotypic characteristics of plant fruiting bodies and detect successful pollination.

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.

The invention concerns a system (1) for the cultivation of a crop (2), comprising rows of elongate growth trays (3, 4, 5) that are situated side by side in a cultivation area having a length direction and a transverse direction, the system comprising a frame (10) extending across one tray or a number of trays (3, 4, 5), and movable in the length direction and / or in the transverse direction, the frame carrying vertically extending pollination members (20, 21, 22) extending from the frame to a predetermined distance from the growth trays and an actuator (25, 26) connected to each pollination member for driving the pollination members (20, 21, 22) and exerting a shaking force on the crop below, wherein the actuator comprises a motor (34, 43, 44) having a vibrating drive member, the pollination member (35, 36, 37, 45, 46) comprising flexible strips or tubes for contacting the crop.