3141 results about "Pollination" patented technology

The invention discloses a breeding method for aseptic seeding and test tube seedling formation of orchids and the adopted broad-spectrum culture medium. In this method, a healthy orchid mother plant is selected, and artificially pollinated when it blooms, and the fruit that develops to maturity after pollination and has not cracked is used as an explant, or the orchid fruit that is basically mature and not cracked is taken from the wild as an explant. Implants undergo reproductive steps such as aseptic sowing, seed germination, strong seedling cultivation, and test-tube seedling transplantation. The specially prepared medium is used for aseptic sowing and seed germination to obtain small plants, and then the small plants are cultivated on the specially prepared strong seedling medium, and the seedlings are hardened under natural light in the greenhouse for 7-14 hours before being released from the bottle. One day, the test-tube seedlings were taken out, the root medium was washed off, and they were planted in a mixed substrate of bark, bluestone and peat, and further cultivated into seedlings. The invention has the characteristics of high seed germination rate, fast seedling growth, good seedling quality, low cost, etc., can obtain a large number of test-tube seedlings in a short period of time, and the transplanting survival rate of the seedlings can be kept above 90%. An effective way can be provided for the production of orchid seedlings.

The invention discloses a thread-guiding-type pepper selfing fruit marking method and belongs to the technical field of plant breeding. The thread-guiding-type pepper selfing fruit marking method comprises the following steps: firstly selecting selfing single plants, and marking by hanging a tag; secondly, selecting flowers about to bloom in the selected selfing single plants; threading a filament into a hand sewing needle with a certain specification (0.2-0.4mm), and tieing a knot at one end close to a needle hole; threading a guiding thread of the hand sewing needle through a pedicel part of each flower to be bloomed; leaving the tail end, about 2-3cm long, of the filament into the pedicel part as a marker of a selfing pollination flower; manually pollinating the marked flowers; and harvesting fruits with filament markers in the pedicel parts in a physiological mature period.

The invention discloses a greenhouse cultivation method for promoting high quality and high yield of the dragon fruit. The method includes steps of construction of greenhouse heat-preserving facility, soil preparation and bed making, frame erecting and modeling, planting, water and fertilizer management, shaping and trimming, artificial pollination, blossom and fruit thinning, pest control and the like. In addition, the invention further discloses a greenhouse structure and a dragon fruit frame building structure. The greenhouse cultivation method and the greenhouse structure for promoting high quality and high yield of the dragon fruit solves problems of high cost, low yield, poor quality and the like in conventional greenhouse cultivation technologies of the dragon fruit, cultivation of the dragon fruit is simplified, production cost is lowered, and the dragon fruit yielded is high in yield, is truly green and healthful fruit and has nutrient value obviously higher than that of the dragon fruit cultivated by conventional methods.

The present invention allows a user to synchronize a device with at least two data sources that may cross-pollinate. The user's device is used to shuttle changes between the sources and resolves conflicts when changes are made to an item on multiple sources concurrently. The user's device keeps track of which version of the item each sync source has and synchronizes each of the sources to the latest version of an item.

A process is described for producing hybrid seed using male-sterile plants created by employing molecular techniques to manipulate antisense DNA and other genes that are capable of controlling the production of fertile pollen in plants. Transformation techniques are used to introduce constructs containing antisense DNA and other genes into plants. Said plants are functionally male-sterile and are useful for the production of hybrid seed by the crossing of said male-sterile plants with pollen from male-fertile plants. Hybrid seed production is simplified and improved by this invention and can be extended to plant crop species for which commercially acceptable hybrid seed production methods are not currently available.

An indoor soilless plant cultivating system, comprising a plurality of stationary light posts, each of which adapted to illuminate a predetermined sector of an indoor facility in accordance with a predetermined illumination signature; a plurality of plant growth towers that are rotatable about a substantially vertical axis in accordance with a predetermined timing sequence so as to be exposable to the light generated at any given time by one or more of the light posts and that are arranged by at least one module defining a module darkened interior region within which plants being instantaneously positioned receive a sensation of nighttime; and irrigation means for supplying the plants being cultivated in each of said towers with a nutrient-rich solution. Moreover, an artificial pollination system based on compressed air nozzles is incorporated within the light posts.

The invention relates to a propagating method of Dendrobium officinale Kimura et Migo seedlings, characterized in that, vigorous Dendrobium officinale Kimura et Migo maternal plant is performed with artificial pollination, the obtained fruit is sanitized and cut, seeds are used for bourgeoning culture medium, causing the seeds to bourgeon protocorm and further form young seedling, then the young seedling propagated in s test tube is cultivated by test tube blossom culture medium, the vigorous plant is selected with artificial pollination in the test tube after blossom, cut after fruit ripening with aseptic seeding, causing the seeds to develop into small plant, which is cultivated on good seedling culture medium, mature shoot is formed, the mature shoot is performed with seedling exercising in the natural light, furthermore cultivated into the seedling. The invention is simple in operation, low in cost, greatly shortened in maturing stage of the fruit of Dendrobium officinale Kimura et Migo, high in efficiency and no plant diseases and insect pests, and transplanting success ratio reaches more than 95%.

The invention belongs to the technical field of plant breeding, and in particular relates to a single flower selfing isolation method for small chili. The method comprises the following steps: (1) selecting an excellent single plant, namely in the flowering period, selecting the excellent single plant according to a breeding objective, and marking; (2) preparing a capsule shell, namely separating a shell cap from a shell body of a hollow hard capsule shell; (3) selecting a flower bud, namely selecting a large flower bud which is expected to bloom in the next day; (4) treating the flower bud, namely holding the flower bud to be treated by one hand, holding the capsule shell, which is matched with the flower bud in size, by the other hand, slightly sleeving the flower bud with the lower opening of the capsule shell over the widest part of the flower bud, and marking a flower stalk; (5) managing after the treatment in a method which is the same as a conventional field management method for the production of small chili. The capsule shell used in the method is low in cost and does not hurt the flower bud and the flower stalk when being used, the operation is convenient and easy, and the work efficiency is high; moreover, the capsule shell with certain elasticity and permeability does not influence the cracking of anther and pollination, and thus the selfing setting rate of the small chili is high.

The invention discloses a method for pollution-free cultivation of pumpkins in a greenhouse, belonging to the technical field of plant cultivation. The method comprises the following steps of: A, seed treatment: a, seed selection; and b, seed soaking to accelerate germination; B, preparation and disinfection of nutritive soil; C, culture of seedlings; D, sowing; E, seedbed temperature management; F, seedbed moisture management; G, soil preparation and spreading of base fertilizer; H, determination of the planting time and method; I, field management: a, temperature and humidity management; b, scaffolding; c, plant adjustment; d, topdressing; e, pollination; J, pest and disease control; and I harvesting. The method for pollution-free cultivation of pumpkins in the greenhouse is scientific and reasonable, the management is perfect, the fertilization is scientific, diseases of fruits can be reduced, the temperature conditions can be well controlled, the survival rate is high, the yield of fruits is high, the quality of the produced fruits is high, and demands of people for pumpkins grown in the greenhouse can be satisfied.

The invention discloses a method for planting kiwi fruits. The method includes the following steps of (1) arranging filed ridges, (2) planting the kiwi fruits and building a frame, (3) shaping the kiwi fruits, (4) clipping the kiwi fruits, (5) carrying out pollination, flower thinning and fruit thinning on kiwi fruit plants, and (6) carrying out watering, pesticide spraying and fertilizing on the kiwi fruits. By means of the method, the kiwi fruit planting method is wide in application range and harmless in growth period, reduces production cost and improves quality of the kiwi fruits.

The invention relates to a method for cross breeding and seedling propagation of paphiopedilum, which comprises the steps of artificial pollination, sterile seeding, strong seedling culture, and test-tube plantlet transplant. After the artificial pollination of paphiopedilum coenospecies, a powdery embryo in hybrid fruits is inoculated to a specially prepared seed germination culture medium for culture; plantlets obtained by the sterile seeding are inoculated to a specially prepared strong seedling culture medium for strong seedling culture; and finally, the test-tube plantlet transplant is performed. The method uses unique culture media, ensures the high germination rate, quick seedling and high seedling quality of paphiopedilum hybrid seeds, has the advantages of high operability, high application value and the like, and overcomes the difficulty that the paphiopedilum hybrid seeds are extremely difficult to germinate in a natural state due to the incomplete development of embryo and other difficulties.

The invention discloses an alpine region solar greenhouse peach cultivation method. The alpine region solar greenhouse peach cultivation method is applicable to peach cultivation in a greenhouse built at the northern latitude of 39 degrees to 40 degrees, and comprises the steps of selecting good peach new species, conducting high-density field planting, conducting high photosynthetic efficiency tree shape cultivation, conducting water and fertilizer standardization management, conducting temperature and humidity management and clipping according to a peach growth period and a time period, and conducting pollination and plant disease and insect pest comprehensive control management at the right moment. By the adoption of the alpine region solar greenhouse peach cultivation method, the yield of peaches per mu can reach more than 3000 kg and is increased by more than 20% compared with the prior art. Soluble solids of peach fruits can reach more than 12% and is increased by two or more percent points compared with the prior art. The proportion of the high-quality peaches is improved to more than 85% and increased by 15 percent points compared with the prior art. The high-quality peaches can come into the market 10 days to 12 days ahead of time, so that more economic benefits are obtained.

The invention provides a fruit tree multi-provenance quality seed breeding method which includes following steps: (1) performing pollination to a fruit tree in A variety through mixed pollen to obtain BC1 hybridized seeds, wherein the mixed pollen is formed by pollens from fruit trees in various varieties which are conspecific with the A variety; (2) breaking the dormancy of the BC1 hybridized seeds, sowing the BC1 hybridized seeds in a greenhouse and culturing the seedlings; and (3) transplanting the seedlings obtained in the step (2) to large fields, and selecting the plants which are excellent in comprehensive characters. The seed breeding method has important value in fruit tree seed breeding.

An apparatus for providing solitary bees for pollination, comprising a bee nest and a portable on-site incubator. The bee nest can comprise a plurality of corrugated sheets, each sheet comprising a plurality of flutes. When the sheets are stacked together, the flutes form cavities arranged in a tessellating pattern. The portable incubator can comprise a chamber for holding dormant adult bees, an opening providing access to the chamber, a passage for exiting of bees from the chamber, means for heating the chamber, means for controlling chamber temperature, and a power supply. Dormant adult bees placed in the incubator are stimulated into activity and leave the incubator. Female bees that leave later lay eggs in the bee nest.

The invention discloses a cultivation method of pear trees. The cultivation method of pear trees includes the first step of selection of a planting site; the second step of fertilizer application, wherein base fertilizer is the basis in combination with topdressing and leaf fertilizer; the third step of pruning, wherein the tree form of pear trees is of a trunk double-layer center opening shape or three-bough natural center opening shape; the fourth step of management of flowers and fruits, wherein the percentage of fertile fruits is improved, flowers and fruits are thinned, fruits are sleeved with bags, pests and diseases are controlled, and water drainage and drought resistance are taken into consideration; the fifth step of harvesting. According to the cultivation method of pear trees, the tree form of pear trees is of the trunk double-layer center opening shape or three-bough natural center opening shape, and artificial pollination is adopted in the flower and fruit management process, so that the percentage of fertile fruits and the effect of flower and fruit thinning are improved, newly planted young pear trees can early bear fruits, and the percentage of fertile fruits is improved, the farm manure is the main fertilizer and applied heavily and cleverly, and therefore the quality of fruits is improved, fruits are big, the yield is high, and economical benefits are good.

The invention discloses a method for breeding a mung bean flowering pollination mutant based on CRI SPR/Cas9 gene editing technology and a special gRNA. The method comprises the following steps of: transferring the CRISPR/Cas9 plasmid of a plant containing a gRNA sequence into a mung bean, and thereby, editing the ORF region of the Cha gene of the mung bean shown in SEQ ID NO. 1, wherein the DNA sequence corresponding to the gRNA is shown in any one of SEQ ID NO. 2 to SEQ NO. 21. According to the invention, a gene for coding the YUCCA protein of the mung bean is edited by using the CRISPR/Cas9gene editing technology, and a new mung bean germplasm with missing keel petals and exposed stigmas and anther is obtained, thus providing a basis for cross breeding of mung beans.

A processing unit for solitary bees is utilized in a method of timely providing a sufficient number of bees for pollinating a flowering crop. The processing unit provides secure storage for the solitary bees at every stage of their life cycle, and provides precise control of environmental conditions to ensure that the bees complete each stage of their life cycle in the optimum state of health and viability, and to ensure that adult bees emerge in close agreement with predicted emergence. The processing unit provides for protection against parasites, predators and pathogens, and may provide for feeding and mating of the bees. The processing unit may be used with any type of solitary bee nest. The processing unit may be fabricated in modularized components, allowing for increased capacity as required.

The invention discloses a gingko planting method and belongs to the technical field of traditional Chinese medicine planting. The method comprises the steps of 1) land selection and preparation including land selection, fertilizer application, land leveling, ploughing and bedding; 2) seedling cultivation including seed collection, breeding nursery garden construction and seedling planting: 3) transplantation and planting including seeding hole digging, fertilizer application, transplantation at a male-female ratio of 95:5, soil covering and watering; 4) field management including intertillage, topdressing, pruning and pollination; 5) insect and disease prevention. According to the gingko planting method, during the leveling process, acid modifier composed of vinegar residue and decomposed straw fertilizer is applied, so that gingko can be planted and grow in saline-alkali soil, and further compared with the situation that saline-alkali soil is unsuitable for growth of gingko in the prior art, the gingko planting method widens the gingko planting area. The gingko planting method also has the advantages of being high in emergence rate, high in seedling disease resistance and the like.

The invention belongs to the technical field of crop cultivation, and particularly relates to a cassava blossoming regulation and control technology. The cassava blossoming regulation and control technology is characterized by comprising selection and preparation of land, plantation management, regulation and control of the blooming period, flower retention, regulation and control of the ratio of female flowers to male flowers, measurement of the pollen activity, pollination experiment and cross breeding. Stable and superior hybrid seeds are obtained by adopting a chemical regulation and control method and a manual agricultural measure regulation and control method. The cassava blossoming regulation and control technology has the characteristics of early blossoming time, high blossoming rate and easiness in technical operation, and is suitable for cross breeding in most cassava planting areas in China.

The invention discloses a highly-efficient planting method of dragon fruits. The highly-efficient planting method of dragon fruits comprises following steps of: (1) standing columns; (2) applying fertilizers; (3) performing field planting; (4) pinching; (5) topdressing; (6), performing pollination; (7) pruning branches; and (8) performing disease and pest control. The highly-efficient planting method of dragon fruits has following beneficial effects: the highly-efficient planting method combines organic fertilizers with inorganic fertilizers by reasonable fertilizing in order to increase utilization rate and decrease manufacturing cost of fertilizers; nutrition demands in different periods of dragon fruits are satisfied; biopesticides are adopted during an overall process in order to prevent diseases and pests without using any pesticide so that fruit without pollution can be made; the highly-efficient planting method helps improve yield and quality of dragon fruits and generates good economic benefit and is worth of promotion.

The invention provides a cultivation method for nutrient-rich green glutinous corns with fruit flavor, comprising the following steps: A, tillage and film covering, namely, ridging, furrowing in the middle of the ridge to bury the fertilizer, and covering the film; B, selecting the seeds from Green Glutinous Corn No.1; C, pre-sowing treatment, namely, sunning the seeds, soaking the seeds and accelerating germination; D, timely sowing within the following sowing time: March 10th to 30th for spring green corns, July 10th to 30th for autumn green corns; E, artificial pollination, that is, when 30% of female plant ears start to develop silk, pollen is artificially ejected once every two days for continuous two to three times; F, fruit setting, that is, only retaining the topside fruit ear of the plant; and G, water management and pest control. The cultivation method has the advantages that the corn cultivated through the method has shorter stalk than the ordinary glutinous corn and is provided with lodging resistance, strong adaptability, better taste than the ordinary glutinous corn, as well as fruit-type apple aroma.

The invention provides a method with effects of saving cost and improving efficiency for liquid pollination of pear tree, which belongs to the fruit trees production technical field. The method comprises the following steps: preparing a nutrient solution in full-bloom stage of pear flowers, adding 0.8g pure pollen in every liter of nutrient solution, spraying and pollinating by an electrostatic sprayer to achieve the purpose of artificial pollination. The pollination method has the advantages of simple operation, fast pollination speed, uniform powder injection, concentration, accuracy, pollen and manual work saving, low cost and the like, and solves the practical problems that pollen can not be uniformly dissolved in water in liquid pollination of fruit trees, pollen is easy to adhere with the wall of a container, pollen is obstructed a nozzle, uneven powder injection causes poor pollination effect and the real application of liquid pollination can not be realized for a long time. According to the invention, the new collected pure pollen (germination rate is 60%-80%) in current year required by per mu is 8-11g. In addition, the method of the invention is capable of increasing the moisture and nutrient of pistil stigma and prolonging the fecundation time of stigma pollination, and the fruit quality can not be affected by increasing the fruit setting rate.

A field management method of high-end kiwi fruit during a fruiting period relates to a cultivation management method which is applied after a kiwi fruit plant can fruit. By systematic treatment of the method comprising application of fertilizer prior to flowering, bud picking, pollination, fruit thinning, application of fruit expanding fertilizer, application of leaf surface fertilizer, pest control, bagging, core picking, drought resistance, drainage of stagnant water, base fertilizer application, winter pruning, winter orchard-clearing disinfection and the like, the kiwi fruit can fully and efficiently utilize light, temperature, water, air and fertilizer, the relations of branch and leaf growth and fruiting are adjusted in real time, distribution of plant nutrient is adjusted, the fruiting capability of the kiwi fruit can be brought into play as far as possible, single-fruit grain weight and the number of contained seeds are increased, pest and disease damage can be eliminated simultaneously, and the disease-resistant capability of plants is enhanced. Besides, the trophic structure of the soil can be adjusted, soil fertility is not reduced all the year round, high quality, high yield and stable yield of the kiwi fruit are achieved, and the age limit of fruiting is prolonged.

Methods for the production of reversibly male-sterile plants by introduction of a polynucleotide encoding a cytokinin oxidase are disclosed along with nucleic acid constructs and transformed cells useful in the production of such plants. Also disclosed are the use of plants containing recombinant nucleic acid sequences in preventing pollination of plants with pollen containing one or more transgenes and in the introduction of economically important traits into elite varieties of plants.

The invention discloses a method for increasing the content of mogroside V. The method comprises the following steps: step I, dissolving jasmonic acid methyl ester into an ethanol solution of which the volume percentage is 20%, and preparing a 50mmol/L ethanol solution of the jasmonic acid methyl ester; dissolving the jasmonic acid methyl ester into ethanol, and preparing a 50-400[mu] mol/L jasmonic acid methyl ester solution; step II, inoculating a momordica grosvenori tissue culture seedling into a solid culture medium, wherein ethanol mother liquor of the jasmonic acid methyl ester is added into the solid culture medium when the solid culture medium is prepared, and thus the final concentration of the jasmonic acid methyl ester is 50-400[mu] mol/L; step III, transplanting the tissue culture seedling into a field, 50-60 days after pollination of momordica grosvenori, spraying the jasmonic acid methyl ester solution once in the morning, at noon and at night every day till water drops off from leaves, and continuously spraying for 10 days. The method is capable of effectively increasing the yield of the mogroside V and has the characteristics of being rapid in effect taking, low in cost, simple to operate, convenient to implement, free of toxin or residue in products and the like, and large-scale production of the mogroside V can be met.