131 results about "Cytokinin" patented technology

The invention relates to the technical field of microorganisms, in particular to paenibacillus mucilaginosus HX-02 and application of the paenibacillus mucilaginosus HX-02 in promoting crop growth. The preservation number of the paenibacillus mucilaginosus HX-02 is CGMCC (China General Microbiological Culture Collection Center) No. 30949. The application comprises increasing one or more of the plant height, the branch number, the weight or the number of crops. According to the invention, the paenibacillus mucilaginosus HX-02 is obtained through screening and separation, and can decompose lattice structures of soil minerals to release K, Fe, Mg, Si, Al and Mo; secreting cytokinin, gibberellin, kinetin, indoleacetic acid and acetylsalicylic acid; therefore, the invention has important application value in the field of agricultural development.

The invention relates to a microbial strain and application thereof, in particular to bacillus brevis, a microbial agent prepared from the bacillus brevis and application of the microbial agent. The invention provides the cold-resistant bacillus subtilis which has multiple functions of generating capsular polysaccharide, being high in salt resistance and cold resistance, promoting growth and disease resistance of plants in saline-alkali soil and the like. The bacterial strain can generate capsular polysaccharide, experiments prove that the bacterial strain has high capabilities of dissolving phosphorus, fixing nitrogen and producing siderophore, IAA, ACCD and cytokinin, and has strong antagonistic action on fusarium, curvularia and microplasmodium, and the generated capsular polysaccharide can promote salt tolerance of plants, so that the yield of the plants is increased, and the yield of the plants is increased. The bio-fertilizer is suitable for production of multifunctional bio-fertilizers with disease prevention, growth promotion, salt and alkali resistance, cold resistance and the like, is applied to production of saline-alkali soil grain crops, pasture and greenhouse commercial crops, has remarkable effects of promoting cold damage resistance, root development, prevention and control of root diseases, improvement of crop yield and the like, can reduce the use of chemical fertilizers and pesticides, and has a wide application prospect.

The invention discloses an application of a populus tomentosa IPT5b gene in creating a high-yield shade-tolerant populus germplasm, belongs to forest breeding, and is characterized in that a phloem specific promoter CLE41b is utilized to express a cytokinin synthetic gene IPT5b in populus tomentosa, and an overexpression material of which cambium activity and xylem development are remarkably promoted is created. After shading treatment, the number of cambium cell layers and the number of xylem cell layers of CLE41bpro: IPT5b are remarkably higher than those of wild type CLE41bpro: IPT5b, and the phenotype increases the content of cytokinin (CK) in stems, so that the wood yield of trees under the shading condition can be increased. According to the method, a high-yield and shade-tolerant new poplar variety is created in a manner of finely regulating and controlling the content of CK in the phloem of the poplar. The gene source and scientific reference are provided for improving and improving the yield and shade tolerance of other trees, and the gene has important application value in the field of forest tree gene engineering.

A method is provided for biologically stimulating growth of and / or regulate enhancing performance plant for agricultural and non-agricultural purposes crop plants and non-crop plants like ornamental plants, industrial material plants, energy biomass production plants, pharmaceutical interest plants, spices, algae, kelp, etc. And beneficial productivity use in aquaculture as hydroponic systems, etc. comprising applying to the plants a DuckWeed.Bio Concentrated Products depending on the target crop customized formulation product and products having an a Auxin IAA or IBA or a combination comprised of Auxins to Cytokinins by biological activity measurement approximate concentration ratio of at least 10:1 to 30:1 and up to 100:1 to 500:1 but not limited to as synergistically can reach much higher levels of ration beyond 1000:1 depending on the compounding formulation mixes of Aquatic Plants and or its extracts.

To provide genetically modified plants capable of inducing root nodulation upon appropriate signaling.SOLUTION: Provided herein is a genetically modified plant comprising NODULE INCEPTION (NIN) and NIN-like protein (NLP) that has been genetically altered to be responsive to cytokinin so that the NIN or NLP protein can induce root nodulation upon an appropriate signaling.SELECTED DRAWING: Figure 4A

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention provides a tomato cytokinin receptor gene SlHK2 and an application of a knockout vector of the tomato cytokinin receptor gene SlHK2 in regulating and controlling the size of tomato fruits. The cytokinin receptor gene SlHK2 is identified from tomatoes. The knockout vector disclosed by the invention is targeted to an sgRNA sequence as shown in SEQ ID NO.1, namely a fragment with the length of 20bp in a 6370-6389bp region of an SlHK2 gene sequence. The invention also provides a transgenic engineering bacterium transfected with the knockout vector of the tomato cytokinin receptor gene SlHK2 and application of the transgenic engineering bacterium in regulation and control of tomato fruit size. According to the invention, a target sgRNA fragment of a CRISPR / Cas9 gene edited tomato cytokinin receptor gene SlHK2 is obtained through screening, a CRISPR / Cas9 gene edited vector pYLCRISPRCas9Pubi-N-SlHK2 of the SlHK2 is obtained through construction, then transgenic engineering bacteria are transfected and transferred into tomatoes, and the size of tomato fruits of an obtained homozygous mutant strain of the SlHK2 gene is obviously increased.

The invention discloses a plant growth regulator used in a rice panicle differentiation stage under high temperature stress and a use method thereof, the plant growth regulator comprises jasmonic acid methyl ester, fertilizer is applied 7 days after rice transplanting (tillering stage) and the panicle differentiation stage respectively, and a shallow water layer is kept after transplanting; spraying the plant growth regulator used in the rice panicle differentiation stage under high temperature stress at the initial stage of rice panicle differentiation; other management is carried out according to conventional field high-yield cultivation management. According to the plant growth regulator, by exogenous spraying of MeJA, the rice yield can be increased by 60% or above, multiple physiological indexes can be improved at the same time, the stress resistance of rice is enhanced, the nutritional requirements of rice spike growth and development are met, damage of high temperature stress to rice is relieved, the rice spikelet differentiation number is increased, and therefore the grain number per spike is increased, and the yield is increased. The method is beneficial for regulating endogenous growth hormones such as cytokinin of rice, optimizing the growth and development process and relieving the influence of high-temperature stress on the morphogenesis of young ears.

The present invention relates to (1) a method for producing biochar having bio stimulating effects, (2) biochar that has been produced to having increased bio stimulating effects, (3) a method for capturing material extracted from biochar and (4) biochar extracts, comprising desired compounds extracted from biochar and suspended in an extract solution. The methods above all involve obtaining a particular biomass source and pyrolyzing the biomass source under specific conditions to increase the bio stimulating effects of the biochar. The method for capturing the material extracted from the biochar produced to have such stimulating effects includes contacting the biochar with a treating liquid, separating the biochar from the biochar extract, collecting the biochar extract from which the biochar has been separated and resuspending the biochar extract in an modified aqueous solution for further use and / or processing separate from, or prior to, disposal. The biochar extract suspended in solution then includes desired compounds (a) at least 0.1 ppm of one or more of the following phenylpropanoid, phenylalanine, flavonoids, sterols, terpenes, terpenoids, tannins, coumarins, benzaldehydes and phenols; (b) at least 0.1 g / L of humics, fulvics or a combination of both humics and fulvics and / or (c) one or more of the following salicylic acid, jasmonic acid, abscisic acid, cytokinin, gibberellic acid and ethylene, by way of example.

The invention discloses a marine sediment bacillus CK22 and application thereof, and belongs to the field of microorganisms, the strain is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20251027. The strain disclosed by the invention has the capabilities of dissolving potassium, dissolving calcium and magnesium and secreting plant growth hormone and cytokinin, particularly has very excellent capability of dissolving magnesium element, is improved by more than 20 times compared with a control group, has excellent comprehensive capability, and can be used for preparing a microbial fertilizer for promoting the growth of crops.

The invention discloses an application of a CKX gene in regulation and control of an alfalfa root system, which comprises the following steps of: 1, preparing a transgenic body of wild alfalfa, and over-expressing an MtCKX1 gene in the alfalfa to obtain a transgenic plant; the cDNA sequence and the amino acid sequence of the MtCKX1 gene are a sequence 1 and a sequence 2 in a sequence table; 2, comparing the root system of the alfalfa transgenic plant with the root system of a wild type plant, wherein the root system of the alfalfa transgenic plant is larger than that of the wild type plant; the cytokinin content of the transgenic plant is inhibited, and the root system of the transgenic plant is enhanced and enlarged; the cytokinin has a negative regulation effect in the aspect of root system growth of plants, after overexpression of the MtCKX1 gene, the cytokinin is inhibited, the root system of the plants is enhanced and enlarged, and the effects of well fixing soil and preventing soil loss are achieved.

The present invention belongs to the field of plant genetic engineering technology, and in particular to a rice cytokinin response regulator gene OsRR24 And application, the rice cytokinin response regulatory factor gene OsRR24 It encodes a protein containing a DNA binding domain and a conserved aspartate-containing signal receiving domain, and is a response regulator downstream of the rice CK signaling pathway. OsRR24 During the functional process in rice, it was transferred into Nipponbare and Bph6 In the NIL, overexpressing plants showed significantly enhanced resistance to brown planthoppers, while RNAi-inhibited plants showed significantly reduced resistance to brown planthoppers. The gene of this invention provides a good theoretical basis for studying how cytokinins participate in rice insect resistance through response regulatory factors. It has reference significance for studying gene molecular functions and also provides genetic resources for the design and breeding of insect-resistant rice varieties.

A plant medium composition and methodology of application of the medium as it pertains to the micropropagation of Cannabis and related plant species is described that yields two to four-fold increase in the number of biologically functional nodes from axillary shoots thereby allowing for exponential amplification of such plant species. The compositions comprise basal plant media supplemented with: (i) one or more cytokinins, and (ii) one or more gibberellins and / or brassinolides. This achievement in exponential shoot growth of these plant species represents a marked step forward in the Cannabis industry as it pertains to commercial micropropagation of a recreationally and medicinally important plant whose extracts are the subject of numerous clinical trials.

The invention discloses a high-temperature-resistant salt-tolerant microbial inoculant culture nutrient solution and a preparation method thereof. The high-temperature-resistant salt-tolerant microbial inoculant culture nutrient solution comprises the following components: 1.0% (w / v) cane sugar or 1.0% (w / v) glucose; 0.5% (w / v) potassium nitrate (KNO3); 0.3% (w / v) of monopotassium phosphate (KH2PO4); 2-3% (w / v) sodium chloride (NaCl); 0.05% (w / v) of nano silicate; 0.1% (w / v) of a seaweed extract; 0.001% (w / v) of carbon nanotubes; 0.002% (w / v) of amino acid chelated iron; 0.2% (w / v) of agarose or 0.2% (w / v) of xanthan gum; 0.01% (w / v) of a mixture of vitamin C and vitamin E; 0.0005% (w / v) of gibberellin and cytokinin; and 0.005% (w / v) HEPES buffer solution. By adding the nano silicate and the carbon nanotubes, the thermal stability is enhanced, microbial cells are protected to survive and breed in a high-temperature environment, the salinity adaptability is improved by the sodium chloride and the seaweed extract, the growth of microorganisms in a high-salt environment is enhanced, the microorganisms can be effectively protected to survive and breed in the high-temperature and high-salt environment, and the survival rate of the microorganisms in the high-temperature and high-salt environment is improved. Sufficient nutritional ingredients are provided to promote rapid growth of microorganisms, meanwhile, the pH value of the nutrient solution is kept stable, and smooth proceeding of the culture process is ensured.

The invention discloses a tissue culture and rapid propagation method of salvia aureocauda, and belongs to the technical field of plant rapid propagation. The method comprises the following steps: firstly, sterilizing a salvia aureocauda tender stem, cutting into stem sections with 2-3 haunches, and sterilizing for later use; inoculating the sterilized salvia aureocauda stem segments on the surface of a solid culture medium containing nutritional ingredients for primary culture; carrying out subculture on the sterile seedlings which are successfully subjected to primary culture on an original culture medium; and finally, transferring the bud seedlings which are successfully subcultured into a hormone-containing 1 / 2 MS or WPM culture medium for rooting culture. According to the method, cytokinin 6-BA is matched with NAA with the proper concentration, WPM + 0.1 mg / L NAA + 0.2 mg / L 6-BA is used as the culture medium, the effect is best, adventitious buds are easily formed, the growth condition is good, the WPM + NAA 0.05 mg / L culture medium is used in the rooting aspect, and the obtained rooting rate is high.

The invention provides a method for inducing butterfly orchid to bloom, which mainly researches the secondary blooming characteristic of butterfly orchid, and promotes the blooming of butterfly orchid and shortens the secondary blooming time of butterfly orchid in combination with the use of a plant growth regulator and an environment management method. According to the technical scheme, the method is mainly used for evenly spraying an exogenous growth regulator on leaf surfaces, the exogenous growth regulator is a solution of gibberellin or cytokinin, methyl jasmonate and indole-3-acetic acid, and meanwhile environment management is matched, so that the purpose of shortening the flowering time of phalaenopsis amabilis is achieved.

The invention provides an anti-cracking agent for preventing seedless litchi fruits from cracking before picking, and particularly relates to the technical field of crop cultivation. The anti-cracking agent for preventing the seedless litchi fruits from cracking before picking comprises a component A, a component B and a component C, the component A is one of thidiazuron or forchlorfenuron, the component B adopts a brassinolide compound, the component C is a nitrate nitrogen fertilizer, and the brassinolide compound is 24-epibrassinolide. The anti-cracking composition has the beneficial effects that thidiazuron and forchlorfenuron in the anti-cracking composition are efficient plant cytokinin and can efficiently promote plant tissue cell division, brassinosteroid compounds with low concentration can remarkably promote crop growth, calcium ammonium nitrate promotes pericarp growth and increases pericarp toughness, the thidiazuron, forchlorfenuron and brassinosteroid compounds are used cooperatively, and the anti-cracking effect is good. The seed-free lychee anti-cracking agent can achieve an obvious synergistic effect on prevention of seed-free lychee cracking, can rapidly promote fruit peel cell division of fruits, enables fruit peels to grow faster, improves the toughness of the fruit peels, and is good in anti-cracking effect before picking.

This invention relates to a composition for improving cherry fruit set rate, and also to an application of this composition, belonging to the field of cherry cultivation technology. The composition for improving cherry fruit set rate of this invention comprises the following components in parts by weight: 40-70 parts gibberellic acid, 45-80 parts cytokinin, 5-15 parts auxin, 25-40 parts water-soluble fertilizer containing amino acids, 20-25 parts auxiliary materials, and 15,000 parts water. The composition of this invention not only provides external nutrition for cherry growth, but is also safe, stable, light in color, and does not contaminate the fruit surface. While ensuring fruit set rate, this invention also reduces physiological fruit drop, solves the problem of petal and calyx refusal, reduces the workload of fruit farmers, and simultaneously improves fruit size and quality.

Mesylates of heterocyclic cytokinins of general formula I:wherein R is selected from the group comprising C3-C15 cycloalkyl, furfuryl, allyl, 4-hydroxy-3-methylbut-2-en-1-yl, 3-methylbut-2-en-1-yl, 4-hydroxy-3-methylbutyl, 4-hydroxy-1,3-dimethylbut-2-en-1-yl, 4-hydroxy-1,3-dimethylbutyl, benzyl, wherein the cycloalkyl, allyl, benzyl and furfuryl may be unsubstituted or optionally substituted with 1 to 3 substituents selected from the group containing hydroxy, halogen, methyl, hydroxymethyl and methoxy, and salts, solvates and addition salts thereof. These compounds are particularly suitable for agricultural and biotechnological use, and their great advantage is increased water solubility as compared to the corresponding free bases of heterocyclic cytokinins.

The invention provides a plant growth regulator for delaying the harvesting time of cut-flower roses as well as a preparation method and application of the plant growth regulator, and belongs to the technical field of fresh cut flower cultivation. The plant growth regulator for delaying the harvesting time of the cut-flower roses comprises the following raw materials: 1-naphthylacetic acid, abscisic acid, cytokinin, a water-soluble fertilizer containing amino acid and monopotassium phosphate. According to the plant growth regulator for delaying the harvest time of the cut-flower roses, provided by the invention, the 1-naphthylacetic acid, the abscisic acid and the cytokinin are compounded together, so that the flower blooming process can be effectively delayed, and the harvest time is delayed. The plant growth regulator takes plant hormones as main raw materials, has the advantages of environmental friendliness, safety, harmlessness, low cost and the like, and further accurately regulates and controls the flower development process of the cut-flower roses by ensuring normal hormone signal transduction and development regulation and control. The growth regulator can effectively delay the harvesting time, powerfully ensures that the cut-flower roses are accurately marketed as required and the quality of the cut-flower roses is improved, and remarkably improves the production benefits and the industrial value.

The present invention relates to methods of improving growth and yield in plants by applying an effective amount of a mixture of(S)-abscisic acid and cytokinin to the plant. The present invention relates to methods of improving stress tolerance in plants by applying an effective amount of a mixture of(S)-abscisic acid and cytokinin to the plant.

The invention discloses a chitosan wrinkle-inhibiting, beautifying and repairing mask and a preparation method thereof. The chitosan wrinkle-inhibiting, beautifying and repairing mask comprises mask liquid and chitosan fiber cloth, the mask liquid consists of a mixed raw material and water, and the mass fraction of the mixed raw material in the mask liquid is 8-30wt%; the mixed raw materials comprise 10-16 parts by weight of carboxymethyl chitosan, 6-8 parts by weight of polyethylene glycol, 6-15 parts by weight of gibberellin, 12-18 parts by weight of cytokinin, 5-6 parts by weight of propylene glycol, 5-6 parts by weight of sodium hyaluronate, 4-5 parts by weight of glycerol, 2-5 parts by weight of sea buckthorn fruit oil, 1-4 parts by weight of sea buckthorn flavone, 4-6 parts by weight of vitamin E and 2-6 parts by weight of corn glutelin amino acid; the composition comprises, by weight, 2-4 parts of beta-glucan, 20-30 parts of dimethyl hydroxyl silicone oil, 3-6 parts of borneol and 1-3 parts of water. The technical problem that an existing chitosan mask still has defects in the aspects of wrinkle inhibiting, beautifying and repairing can be solved.

The invention discloses application of Cladosporium halotolerans in plant growth promotion, and belongs to the technical field of microorganisms, the Cladosporium halotolerans is Cladosporium halotolerans 550W1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.41943. The invention further discloses a preparation method of the Cladosporium halotolerans. The application of the Cladosporium halotolerans in plant growth promotion belongs to the technical field of microorganisms. According to the salt-tolerant cladosporium sp. 550W1 provided by the invention, plant growth can be promoted by synthesizing auxin, gibberellin, cytokinin and other plant hormones; meanwhile, the salt-tolerant cladosporium sp. 550W1 has the outstanding advantages of being high in environmental adaptability, environmentally friendly, high in safety and the like, and an excellent core strain resource is provided for developing novel microbial fertilizers and promoting green sustainable agriculture.

The invention relates to application of streptomyces bacteria A6 in promoting green keeping of green plants, and belongs to the technical field of microorganisms and application thereof, the streptomyces bacteria A6 is classified and named as Streptomyces roseofulvus and is preserved, the preservation number is CCTCC NO: M20231638, and the preservation number is CCTCC NO: M20231638. The streptomyces bacterium promotes green keeping of green plants through double effects of generating cytokinin and enhancing nitrification to promote the plants to secrete cytokinin, and has the advantages of low production cost, convenience in use and the like.

The invention discloses an application of a CKX gene in regulating alfalfa root systems, comprising the following steps: step 1, preparing a transgenic wild-type alfalfa, overexpressing the MtCKX1 gene in the alfalfa, and obtaining a transgenic plant; the cDNA sequence and amino acid sequence of the MtCKX1 gene are sequence 1 and sequence 2 in the sequence table; step 2, comparing the root systems of the transgenic alfalfa plant with those of the wild-type plant, and the root systems of the transgenic alfalfa plant are larger than those of the wild-type plant; the cytokinin content of the transgenic plant is suppressed, and the root systems of the transgenic plant are strengthened and enlarged; cytokinin has a negative regulatory effect on the root growth of the plant, and after the MtCKX1 gene is overexpressed, the cytokinin is suppressed, and the root system of the plant is strengthened and enlarged, thereby effectively fixing the soil and preventing soil loss.

This invention discloses a composition for prolonging the flowering period of durian and a method for regulating the flowering period of durian. The composition includes: a first spray agent, consisting of the following components in the indicated weight ratios: 5-20g of cytokinin-like substances; 1-5g of gibberellin-like substances; 10-30g of polyamine-like substances; 5-15g of boron source; 100-300g of energy-regulating components; 1-5g of slow-release carrier and penetration-promoting components; and water to 1000g; and a second spray agent, consisting of the following components in the indicated weight ratios: 20-50g of flowering rhythm-regulating components, which are composed of 5-10g of paclobutrazol, 3-8g of uniconazole, and 0.5-2g of chlorpyrifos; 2-5g of slow-release carrier and penetration-promoting components; and water to 1000g. This invention, through the combined design of the first spray agent (flowering promoter) and the second spray agent (flowering control), transforms the initiation of durian flower buds from a "single trigger" to a "controllable, phased trigger," thereby prolonging the flowering period while maintaining or even improving fruit-setting capacity.

The invention relates to the field of microbial preparations, and discloses a microbial preparation with crop transpiration resistance and application, the microbial preparation comprises the following components by weight: 20-30 parts of Piriformospora indica, 15-25 parts of Bifidobacterium, 25-35 parts of endophytic fungi, 8-12 parts of auxin, 3-7 parts of cytokinin, 3-7 parts of trace elements, and 2-8 parts of organic matter; according to the microbial preparation for resisting the transpiration of the traditional Chinese medicinal materials, provided by the invention, by combining the alternaria alternata, the piriformospora indica and the bifidobacterium, the stress resistance and the growth efficiency of the traditional Chinese medicinal materials are remarkably enhanced, and the transpiration resistance of the traditional Chinese medicinal materials is enhanced; the method has the advantages that the nutrient absorption and water utilization efficiency of the traditional Chinese medicinal materials is improved by improving a rhizosphere microbial ecological system, so that the optimal growth state is kept in drought or barren soil.

The embodiment of the invention provides a golden camellia suspension cell culture method which comprises the following steps: golden camellia leaves are collected and inoculated into a WPM liquid culture medium containing hormone for culture, primary callus is formed through induction, and the hormone comprises naphthylacetic acid and 6-benayl aminopurine; and inoculating the primary callus into a WPM liquid culture medium containing auxin and cytokinin, and culturing to obtain the golden camellia suspension cells.

The invention discloses a malania oleifera seed germination accelerating method and application thereof, and the method comprises the following steps: (1) applying mechanical force to malania oleifera seeds, so as to generate cracks on seed shells of malania oleifera to conduct the inner space of the seed shells and the outer part of the seed shells; the crack longitudinally extends on the seed shell and penetrates through the top of the seed shell; (2) soaking the seeds treated in the step (1) in an aqueous solution containing a dormancy breaking agent; the dormancy relieving agent comprises gibberellin and a cytokinin analogue; and (3) sowing the seeds treated in the step (2) in a breathable and water-containing culture medium to perform stratification and germination acceleration, and performing temperature control and regular irrigation in the period until the seeds germinate. According to the application, the method is applied to germination acceleration of malania oleifera seeds collected from wild malania oleifera plants in Guangxi Zhuang Autonomous Region and / or Yunnan.

The invention relates to the technical field of bud grafting of big rubber trees, in particular to a method for increasing the survival rate of bud grafting of big rubber trees, which comprises the following steps: S1, taking big rubber trees as stocks, forming bud joints on barks, smearing calcium chloride at glue outlet positions at the edges of the bud joints, and after glue at the glue outlet positions is solidified, covering the bud joints with the calcium chloride; spraying a bactericide and cytokinin at bud interfaces; s2, placing bud slices to be grafted at positions 1-2cm away from the lower cut of the stock and 0.5-1cm away from the left cut and the right cut of the stock, and sealing and fixing. According to the bud grafting method for the big rubber tree, the breeding time can be obviously shortened, and the production cost can be reduced. For example, establishment of a rubber tree seed orchard takes at least six years from seedling raising to seed harvesting, while seed harvesting can be realized in three years through a big tree grafting technology, so that the seed harvesting age limit is remarkably shortened, and the time cost and the management cost are saved.