40 results about "Cytokinin" patented technology

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention provides a method for inducing butterfly orchid to bloom, which mainly researches the secondary blooming characteristic of butterfly orchid, and promotes the blooming of butterfly orchid and shortens the secondary blooming time of butterfly orchid in combination with the use of a plant growth regulator and an environment management method. According to the technical scheme, the method is mainly used for evenly spraying an exogenous growth regulator on leaf surfaces, the exogenous growth regulator is a solution of gibberellin or cytokinin, methyl jasmonate and indole-3-acetic acid, and meanwhile environment management is matched, so that the purpose of shortening the flowering time of phalaenopsis amabilis is achieved.

This invention relates to a composition for improving cherry fruit set rate, and also to an application of this composition, belonging to the field of cherry cultivation technology. The composition for improving cherry fruit set rate of this invention comprises the following components in parts by weight: 40-70 parts gibberellic acid, 45-80 parts cytokinin, 5-15 parts auxin, 25-40 parts water-soluble fertilizer containing amino acids, 20-25 parts auxiliary materials, and 15,000 parts water. The composition of this invention not only provides external nutrition for cherry growth, but is also safe, stable, light in color, and does not contaminate the fruit surface. While ensuring fruit set rate, this invention also reduces physiological fruit drop, solves the problem of petal and calyx refusal, reduces the workload of fruit farmers, and simultaneously improves fruit size and quality.

Mesylates of heterocyclic cytokinins of general formula I:wherein R is selected from the group comprising C3-C15 cycloalkyl, furfuryl, allyl, 4-hydroxy-3-methylbut-2-en-1-yl, 3-methylbut-2-en-1-yl, 4-hydroxy-3-methylbutyl, 4-hydroxy-1,3-dimethylbut-2-en-1-yl, 4-hydroxy-1,3-dimethylbutyl, benzyl, wherein the cycloalkyl, allyl, benzyl and furfuryl may be unsubstituted or optionally substituted with 1 to 3 substituents selected from the group containing hydroxy, halogen, methyl, hydroxymethyl and methoxy, and salts, solvates and addition salts thereof. These compounds are particularly suitable for agricultural and biotechnological use, and their great advantage is increased water solubility as compared to the corresponding free bases of heterocyclic cytokinins.

The invention provides a plant growth regulator for delaying the harvesting time of cut-flower roses as well as a preparation method and application of the plant growth regulator, and belongs to the technical field of fresh cut flower cultivation. The plant growth regulator for delaying the harvesting time of the cut-flower roses comprises the following raw materials: 1-naphthylacetic acid, abscisic acid, cytokinin, a water-soluble fertilizer containing amino acid and monopotassium phosphate. According to the plant growth regulator for delaying the harvest time of the cut-flower roses, provided by the invention, the 1-naphthylacetic acid, the abscisic acid and the cytokinin are compounded together, so that the flower blooming process can be effectively delayed, and the harvest time is delayed. The plant growth regulator takes plant hormones as main raw materials, has the advantages of environmental friendliness, safety, harmlessness, low cost and the like, and further accurately regulates and controls the flower development process of the cut-flower roses by ensuring normal hormone signal transduction and development regulation and control. The growth regulator can effectively delay the harvesting time, powerfully ensures that the cut-flower roses are accurately marketed as required and the quality of the cut-flower roses is improved, and remarkably improves the production benefits and the industrial value.

This invention discloses a composition for prolonging the flowering period of durian and a method for regulating the flowering period of durian. The composition includes: a first spray agent, consisting of the following components in the indicated weight ratios: 5-20g of cytokinin-like substances; 1-5g of gibberellin-like substances; 10-30g of polyamine-like substances; 5-15g of boron source; 100-300g of energy-regulating components; 1-5g of slow-release carrier and penetration-promoting components; and water to 1000g; and a second spray agent, consisting of the following components in the indicated weight ratios: 20-50g of flowering rhythm-regulating components, which are composed of 5-10g of paclobutrazol, 3-8g of uniconazole, and 0.5-2g of chlorpyrifos; 2-5g of slow-release carrier and penetration-promoting components; and water to 1000g. This invention, through the combined design of the first spray agent (flowering promoter) and the second spray agent (flowering control), transforms the initiation of durian flower buds from a "single trigger" to a "controllable, phased trigger," thereby prolonging the flowering period while maintaining or even improving fruit-setting capacity.

The embodiment of the invention provides a golden camellia suspension cell culture method which comprises the following steps: golden camellia leaves are collected and inoculated into a WPM liquid culture medium containing hormone for culture, primary callus is formed through induction, and the hormone comprises naphthylacetic acid and 6-benayl aminopurine; and inoculating the primary callus into a WPM liquid culture medium containing auxin and cytokinin, and culturing to obtain the golden camellia suspension cells.

The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS / WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

The invention provides an efficient asexual propagation method of ficus pandurata suitable for simple condition application. The method comprises the following steps: (1) explant disinfection: soaking a stem with an axillary bud in a composite disinfectant containing mercuric chloride; (2) primary culture: inoculating the disinfected stem into a starting culture medium containing cytokinin and auxin for culture; (3) multiplication culture: transferring the callus formed by primary culture to a differential culture medium to induce cluster buds; (4) carrying out vector rooting, namely cutting robust buds obtained by multiplication culture, and inserting the robust buds into a rooting culture medium containing microencapsulated auxin; and (5) transplanting and hardening seedlings: treating the tissue culture seedlings with a protective solution, and transplanting the treated tissue culture seedlings to a compound matrix. The method can realize substantive transformation from a specialized tissue culture technology to basic-level production, and solves the industrialization bottlenecks such as equipment threshold and operation complexity.

The present application relates to the field of plant growth regulator technology, and in particular, to a phenylurea compound, a method for preparing same, and use thereof, and a composition for promoting crop cell division and a method for using same. The phenylurea compound provided herein has cytokinin-like biological activity and can effectively promote the germination of crop seeds and the expansion of cotyledons, increase the chlorophyll content, and retain and enlarge the fruits, featuring a stable action effect, safety to crops, and the like. Experimental results show that the phenylurea compound of the present application can effectively promote the germination of rice and peanut seeds, expansion of radish and cucumber cotyledons, leaf pigment retention and yield increase of peanuts, fruit set and retention of zucchini, and enlargement and sweetness enhancement of grape fruits, etc., exhibiting cytokinin-like effects.

The invention relates to the technical field of plant cultivation, in particular to a tissue culture and plant regeneration method for bitter herbs. The method comprises the following steps: S1, preparing an explant: carrying out surface sterilization on seeds of the bitter bugleweed, inoculating the seeds into a first culture medium for culturing to obtain sterile seedlings, and selecting terminal buds or lateral buds of the sterile seedlings as the explant; s2, callus induction: inoculating the explant into a second culture medium for culture, and inducing to form a callus; s3, cluster bud induction and proliferation: inoculating the explant after the callus tissue is formed into a third culture medium for culture, and performing induced differentiation to form cluster buds; s4, rooting culture: separating the cluster buds obtained in the step S3, inoculating the cluster buds into a fourth culture medium for culture, and inducing rooting to form a complete plant. According to the application, auxin substances and cytokinin substances in a specific ratio are used in different stages, so that high efficiency and high stability of plant regeneration of the bitter herbs are guaranteed.

The invention relates to the technical field of plant biology, and discloses a lilium lancifolium virus-free seedling stem tip stripping culture method which sequentially comprises the following steps: (1) pretreatment: taking lilium lancifolium virus-carrying explants, carrying out first high-temperature stress treatment, then transferring into a sub-high-temperature condition, and carrying out second high-temperature stress treatment on the lilium lancifolium virus-carrying explants to obtain lilium lancifolium virus-free seedlings; carrying out shake culture by using a pretreatment culture medium containing plant stress resistance induction hormone; (2) stem tip stripping: after pretreatment in the step (1), stripping stem tip meristem with the size of 0.8-1.2 mm; (3) induction culture: the stem tip meristem stripped in the step (2) is inoculated to a solid induction culture medium for dark culture until callus is formed, and the solid induction culture medium contains cytokinin and an antiviral compound; through deep synergy of gradient temperature stress (high temperature to sub-high temperature) and specific stress-resistant hormone (BR) induction in time sequence and physiological functions, the physiological state of the explant in the pretreatment process is integrally regulated and controlled.

The invention relates to the technical field of gene engineering, in particular to a cotton gene GhAGL15 and application thereof. The GhAGL15 gene is identified in cotton, overexpression of GhAGL15 can significantly promote embryogenic callus induction of cotton, accelerate somatic embryo formation and improve the overall regeneration efficiency, hormone level determination finds that gibberellin in a GhAGL15 knockout strain is significantly accumulated, the cytokinin level in an overexpression strain is significantly increased, and the GhAGL15 knockout strain can be used as a GhAGL15 gene. It is prompted that GhAGL15 can play a role by regulating and controlling metabolism or signal pathways of gibberellin and cytokinin by regulating and controlling far-end regulation and control areas of related genes of gibberellin and cytokinin. And a basis is provided for breeding excellent cotton varieties.

This invention relates to compositions and methods for modifying Cytokinin Receptor Histidine Kinase (HK) genes in plants, optionally to improve yield traits, the HK genes encoding a histidine kinase (HK) polypeptide. The invention further relates to plants having increased improved yield traits produced using the methods and compositions of the invention.

The invention relates to the technical field of fruit tree cross breeding, and discloses a method for obtaining seeds through artificial pollination of figs. The method comprises the following steps: treating Caprifig type figs by using a plant growth regulator to obtain pollen, and transferring the pollen into fig stigmas by using an artificial pollination dry powder method to obtain seeds. The method comprises the following steps: applying a cytokinin plant growth regulator at the stage that the fruit diameter of the Caprifig type figs is less than 3cm for treatment; harvesting mature Caprifig type fruits, separating anther, drying in the shade, and sieving to obtain pollen; fully mixing pollen and starch to form a pollen working agent in a dry powder state; at the stage that the diameter of the pollinated fig fruit is 1-3 cm, a pollen working agent is sprayed into a fruit cavity through a bract opening, so that the pollen working agent is attached to the stigma of a female flower; after the fruits are ripe, harvesting and water-selecting to obtain seeds. By means of the method, pollen with vitality can be obtained, artificial pollination and fruiting of the figs can be achieved, and the method is suitable for fig cross breeding and fruit improvement.

This invention relates to compositions and methods for modifying Cytokinin Receptor Histidine Kinase (HK) genes in plants, optionally to improve yield traits, the HK genes encoding a histidine kinase (HK) polypeptide. The invention further relates to plants having increased improved yield traits produced using the methods and compositions of the invention.

The invention discloses a methylobacterium DS15, a microbial inoculum thereof and application of the methylobacterium DS15 in improvement of yield and quality of grapes and pears. The methylobacterium DS15 is classified and named as Methylobacterium sp., and the preservation number of the methylobacterium DS15 is CGMCC (China General Microbiological Culture Collection Center) No.36115. A bacterial colony of the methylobacterium DS15 is orange red, round and upwards protruded, the edge is neat, and the surface is in a film shape. The methylobacterium DS15 has the capabilities of synthesizing cytokinin, secreting siderophores and solubilizing phosphorus, the methylobacterium and the microbial inoculum prepared from the methylobacterium can remarkably promote yield increase and quality improvement of grapes and pears, the application effect is stable, and the methylobacterium DS15 has important significance on protection of the farming environment and green agricultural development.

The application provides a raspberry tea high-yield cultivation planting management method, relates to the raspberry tea planting technical field, and mainly adopts urea, plant cell mitogen, raspberry tea extract, 1,8-eucalyptol and polyglutamic acid as nutrient agent components for foliar spraying; adopts brassinolide, aspirin and Huaba No.1 as a root soaking solution to perform root (branch) soaking treatment on raspberry tea seedlings before transplanting or cutting; after transplanting and planting, conventional topdressing is performed, and nutrient agent is sprayed on leaves in time after leaf picking. The application overcomes the defects of the prior art, can effectively promote raspberry tea rooting, germination and vine growth, improve the bud tip and stem leaf yield of raspberry tea, and comprehensively improve the economic benefit of planting raspberry tea.

The invention provides a novel anti-cracking regulation and control method for stone fruits of prune plums, and relates to the technical field of fruit tree cultivation and pest control. According to the method, the osmotic pressure and the growth rhythm of fruits are regulated and controlled by adopting a composite reagent of cytokinin, gibberellin, diethyl aminoethyl hexanoate and an osmotic regulation substance at two key nodes of 10-15 days after flower abortion, the end of a hard core period and the start of a second expansion period, and four physical measures of organic fertilizer application, orchard grass growing, ridging and drainage and post-raining water removal are combined. The growth speed of peel is slightly higher than that of pulp, and tree moisture supply and demand are stable. The core innovation lies in osmotic pressure saturation regulation technology, peel and pulp growth rhythm matching regulation and physical and chemical synergistic anti-cracking. Practice verifies that the fruit cracking rate of prunes and plums is reduced to be within 5% from 80%-90%, the commodity rate of fruits is larger than or equal to 95%, a rainproof shed does not need to be built, the cost is reduced by 60% or above, operation is easy and convenient, popularization is easy, and the method is suitable for cultivation of prunes and plums in different regions such as Xinjiang and Yunnan.

The invention provides an application of a cytokinin response regulatory factor ZmARR2 gene and an encoding protein thereof in regulating plant salt tolerance, and belongs to the field of plant genetic engineering breeding. The invention discloses the key effect of ZmARR2 in regulating and controlling the salt tolerance of corn for the first time, and compared with a wild type, a ZmARR2 overexpression strain shows stronger salt stress tolerance, and the survival rate in the seedling stage is obviously improved; on the contrary, gene editing strains and EMS mutants with deletion of functions are more sensitive to salt stress, and the survival rate is remarkably reduced. The ZmARR2 is an important positive regulatory factor in plant salt tolerance, and the research result provides valuable gene resources and theoretical basis for further analyzing a plant salt tolerance molecular mechanism and developing a new breeding strategy for improving the plant salt tolerance, and has important significance for guaranteeing seedling formation of crops in a salinization region. And a new research direction is provided for finally improving the yield stability.

The invention relates to the technical field of genetic transformation of medicago ruthenica, and discloses a genetic transformation method of medicago ruthenica, a target gene is an MrAGL8 gene or an MrPdh1 gene, and the genetic transformation method comprises the following steps: S1, taking a hypocotyl of a medicago ruthenica aseptic seedling as an explant, and infecting the explant by using an agrobacterium resuspension bacterial liquid carrying a target gene expression vector to obtain an agrobacterium resuspension bacterial liquid; carrying out co-culture on the infected explants; the co-cultured explants are subjected to degerming and screening in a differentiation screening culture medium containing antibiotics and cytokinin, and resistance buds are directly induced and differentiated; transferring the resistant buds growing to a certain height into a rooting culture medium to induce rooting, and carrying out seedling hardening to obtain a complete transgenic medicago ruthenica plant; and S2, screening and identifying transgenic plants: carrying out PCR identification and sequencing identification on target genes of the transgenic medicago ruthenica plants. According to the scheme, a complete plant subjected to genetic transformation of the medicago ruthenica is obtained for the first time, and powerful technical support is provided for gene function research of the medicago ruthenica and research and development of new germplasm of the medicago ruthenica.

The present application relates to the technical field of pesticide nano-suspension, and discloses a kind of purine cell division factor nano-suspension, preparation method and application, the nano-suspension includes purine cell division factor raw drug, dispersant and water, the dispersant is the poly carboxylic acid system comb high molecular polymer with number average molecular weight 10000~20000 g / mol, and its solvated side chain is polyether chain segment;The mass ratio of purine cell division factor raw drug and dispersant is 1∶(1.4~1.8).The nano-suspension provided in the present application is superior to the existing preparation of purine cell division factor in biological activity, and its pH value range is 6~7, and it can be mixed with most pesticides, without worrying about the decrease of drug efficacy caused by flocculation and stratification.The present application avoids using polar solvent with safety risk and dangerous chemicals with strong acidity and alkalinity, ensures the safety of environment and human body, and the preparation method is simple and easy to implement, which is beneficial to industrial application.

The present application relates to the application of 4-methoxysalicylic acid and its soluble salt as a cell division-like substance and to the promotion of plant growth and development. The present application uses indoor tests such as lettuce seed germination, lettuce hypocotyl elongation, radish cotyledon expansion, and field tests to comprehensively study the influence of 4-methoxysalicylic acid and its soluble salt on plant growth and development. The indoor test results show that 4-methoxysalicylic acid has a promoting effect on the germination of lettuce seeds, an inhibiting effect on the elongation of lettuce hypocotyls, and a promoting effect on the expansion of radish cotyledons, and it can be preliminarily determined that it has a function similar to that of a cell division-like substance. Further field tests show that 4-methoxysalicylic acid and its soluble salt can effectively promote the absorption and utilization of fertilizer by corn and peanuts, and can effectively regulate the growth of cucumber and citrus fruits, showing an agricultural beneficial physiological effect, and having development and utilization value and application prospect in agricultural production.

The invention discloses a regulator for promoting melon to blossom and bear fruits, and belongs to the technical field of plant growth regulation. The regulator for promoting the melon to blossom and bear fruits is prepared from the following raw materials in parts by mass: 2 to 3 parts of spermidine, 2 to 3 parts of gibberellin, 4 to 6 parts of cytokinin, 2 to 3 parts of ethephon, 4 to 5 parts of seaweed extract, 3 to 4 parts of phytosterol, 10 to 15 parts of xanthan gum and 6 to 8 parts of vitamin E. The regulator can improve the flower bud differentiation effect of the muskmelons, and solves the problems of poor growth and development, unstable fruit setting, yield reduction and the like of the muskmelons under low-temperature stress. In addition, the regulator is long in lasting period and stable in effect, and the use frequency can be reduced under the condition that the effective concentration of the growth regulating substance is guaranteed.

The invention provides a composition for solving the blind branch phenomenon of cut-flower roses as well as a preparation method and application of the composition, and belongs to the technical field of fresh cut flower cultivation. Comprising the following raw materials: gibberellin, cytokinin, diethyl aminoethyl hexanoate, an amino acid water-soluble fertilizer and monopotassium phosphate, the raw materials are compounded together for application, and compound leaves on axillary bud parts of Chinese roses are removed, so that the cut-flower Chinese roses sprout quickly, the phenomenon of blind branches of the cut-flower Chinese roses is reduced, and normal growth and development of the soilless culture cut-flower Chinese roses are ensured. The method is simple to operate, quick in effect, low in cost, environment-friendly and safe, and the industrial problem of the blind branch phenomenon of the cut rosa chinensis is effectively solved.

The application discloses application of a salt-tolerant branch mold in promoting plant growth and belongs to the technical field of microorganisms. Cladosporium halotolerans 550W1, which is preserved in the China General Microbiological Culture Collection Center and has a preservation number of CGMCC No.41943. The salt-tolerant branch mold 550W1 provided by the application can promote plant growth by synthesizing plant hormones such as auxin, gibberellin and cytokinin; meanwhile, the salt-tolerant branch mold 550W1 has the outstanding advantages of strong environmental adaptability, ecological friendliness and high safety, and provides excellent core strain resources for development of a new type of microbial fertilizer and promotion of green and sustainable agriculture.

The invention relates to a method for preventing or reducing plant gall formation, the method comprising applying on a plant a composition comprising a suitable amount of at least one ingredient selected from the group consisting of cytokinins, auxins, or auxin precursors. The auxin is preferably indole-3-butyric acid (IBA). The invention further relates to a method for treating seeds. The invention further relates to the use of a composition comprising a suitable amount of an auxin or an auxin precursor, for the prevention and / or reduction of plant gall formation. The gall formation is preferably caused by clubroot disease.

The invention provides bacillus subtilis JTCS-01 and application thereof, and belongs to the field of microorganisms. The preservation number of the bacillus subtilis JTCS-01 is CGMCC (China General Microbiological Culture Collection Center) NO.37197, the preservation address of the bacillus subtilis JTCS-01 is China General Microbiological Culture Collection Center, and the preservation time of the bacillus subtilis JTCS-01 is December 25, 2025. The strain disclosed by the invention can efficiently secrete plant growth regulators such as indoleacetic acid and cytokinin at the same time and generate antibacterial substances such as lipopeptide antibiotics, so that multiple functions of promoting plant growth, preventing and treating fungal diseases and improving a soil micro-ecological environment are integrated, and a powder or liquid fungicide developed on the basis of the strain has a good application prospect. The fertilizer can be applied to seed treatment, root irrigation and soil application.

This invention provides the application of cytokinin-responsive factors in improving the resistance of potatoes to low-temperature stress, relating to the field of biotechnology. This invention is the first to identify the CRF family in potatoes. Through collinearity analysis and conserved domain screening, nine CRF family members were successfully identified from the potato genome. Functional studies of the CRF family members in potatoes revealed that StCRF3a plays a functional role in low-temperature stress in potatoes. Specifically, StCRF3a is induced by low temperature, and its promoter region contains a low-temperature responsive element.

The invention relates to the technical field of medicine preparation, in particular to application of cytokinin oTR in preparation of a medicine for enhancing tumor cell radiosensitivity. By constructing a stable tumor radiation resistance cell model and combining molecular biology experiments and statistical analysis, it is proved that cytokinin oTR can regulate and control expression of SIRT1 and miR-34a in radiation resistance cells in a time-dose dependence mode, and the radiotherapy sensitization effect is achieved through a p53 / miR-34a / SIRT1 pathway.