75 results about "Cytokinin" patented technology

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention discloses a marine sediment bacillus CK22 and application thereof, and belongs to the field of microorganisms, the strain is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20251027. The strain disclosed by the invention has the capabilities of dissolving potassium, dissolving calcium and magnesium and secreting plant growth hormone and cytokinin, particularly has very excellent capability of dissolving magnesium element, is improved by more than 20 times compared with a control group, has excellent comprehensive capability, and can be used for preparing a microbial fertilizer for promoting the growth of crops.

The present invention belongs to the field of plant genetic engineering technology, and in particular to a rice cytokinin response regulator gene OsRR24 And application, the rice cytokinin response regulatory factor gene OsRR24 It encodes a protein containing a DNA binding domain and a conserved aspartate-containing signal receiving domain, and is a response regulator downstream of the rice CK signaling pathway. OsRR24 During the functional process in rice, it was transferred into Nipponbare and Bph6 In the NIL, overexpressing plants showed significantly enhanced resistance to brown planthoppers, while RNAi-inhibited plants showed significantly reduced resistance to brown planthoppers. The gene of this invention provides a good theoretical basis for studying how cytokinins participate in rice insect resistance through response regulatory factors. It has reference significance for studying gene molecular functions and also provides genetic resources for the design and breeding of insect-resistant rice varieties.

The invention provides a method for inducing butterfly orchid to bloom, which mainly researches the secondary blooming characteristic of butterfly orchid, and promotes the blooming of butterfly orchid and shortens the secondary blooming time of butterfly orchid in combination with the use of a plant growth regulator and an environment management method. According to the technical scheme, the method is mainly used for evenly spraying an exogenous growth regulator on leaf surfaces, the exogenous growth regulator is a solution of gibberellin or cytokinin, methyl jasmonate and indole-3-acetic acid, and meanwhile environment management is matched, so that the purpose of shortening the flowering time of phalaenopsis amabilis is achieved.

This invention relates to a composition for improving cherry fruit set rate, and also to an application of this composition, belonging to the field of cherry cultivation technology. The composition for improving cherry fruit set rate of this invention comprises the following components in parts by weight: 40-70 parts gibberellic acid, 45-80 parts cytokinin, 5-15 parts auxin, 25-40 parts water-soluble fertilizer containing amino acids, 20-25 parts auxiliary materials, and 15,000 parts water. The composition of this invention not only provides external nutrition for cherry growth, but is also safe, stable, light in color, and does not contaminate the fruit surface. While ensuring fruit set rate, this invention also reduces physiological fruit drop, solves the problem of petal and calyx refusal, reduces the workload of fruit farmers, and simultaneously improves fruit size and quality.

Mesylates of heterocyclic cytokinins of general formula I:wherein R is selected from the group comprising C3-C15 cycloalkyl, furfuryl, allyl, 4-hydroxy-3-methylbut-2-en-1-yl, 3-methylbut-2-en-1-yl, 4-hydroxy-3-methylbutyl, 4-hydroxy-1,3-dimethylbut-2-en-1-yl, 4-hydroxy-1,3-dimethylbutyl, benzyl, wherein the cycloalkyl, allyl, benzyl and furfuryl may be unsubstituted or optionally substituted with 1 to 3 substituents selected from the group containing hydroxy, halogen, methyl, hydroxymethyl and methoxy, and salts, solvates and addition salts thereof. These compounds are particularly suitable for agricultural and biotechnological use, and their great advantage is increased water solubility as compared to the corresponding free bases of heterocyclic cytokinins.

The invention provides a plant growth regulator for delaying the harvesting time of cut-flower roses as well as a preparation method and application of the plant growth regulator, and belongs to the technical field of fresh cut flower cultivation. The plant growth regulator for delaying the harvesting time of the cut-flower roses comprises the following raw materials: 1-naphthylacetic acid, abscisic acid, cytokinin, a water-soluble fertilizer containing amino acid and monopotassium phosphate. According to the plant growth regulator for delaying the harvest time of the cut-flower roses, provided by the invention, the 1-naphthylacetic acid, the abscisic acid and the cytokinin are compounded together, so that the flower blooming process can be effectively delayed, and the harvest time is delayed. The plant growth regulator takes plant hormones as main raw materials, has the advantages of environmental friendliness, safety, harmlessness, low cost and the like, and further accurately regulates and controls the flower development process of the cut-flower roses by ensuring normal hormone signal transduction and development regulation and control. The growth regulator can effectively delay the harvesting time, powerfully ensures that the cut-flower roses are accurately marketed as required and the quality of the cut-flower roses is improved, and remarkably improves the production benefits and the industrial value.

The invention discloses a chitosan wrinkle-inhibiting, beautifying and repairing mask and a preparation method thereof. The chitosan wrinkle-inhibiting, beautifying and repairing mask comprises mask liquid and chitosan fiber cloth, the mask liquid consists of a mixed raw material and water, and the mass fraction of the mixed raw material in the mask liquid is 8-30wt%; the mixed raw materials comprise 10-16 parts by weight of carboxymethyl chitosan, 6-8 parts by weight of polyethylene glycol, 6-15 parts by weight of gibberellin, 12-18 parts by weight of cytokinin, 5-6 parts by weight of propylene glycol, 5-6 parts by weight of sodium hyaluronate, 4-5 parts by weight of glycerol, 2-5 parts by weight of sea buckthorn fruit oil, 1-4 parts by weight of sea buckthorn flavone, 4-6 parts by weight of vitamin E and 2-6 parts by weight of corn glutelin amino acid; the composition comprises, by weight, 2-4 parts of beta-glucan, 20-30 parts of dimethyl hydroxyl silicone oil, 3-6 parts of borneol and 1-3 parts of water. The technical problem that an existing chitosan mask still has defects in the aspects of wrinkle inhibiting, beautifying and repairing can be solved.

The invention discloses application of Cladosporium halotolerans in plant growth promotion, and belongs to the technical field of microorganisms, the Cladosporium halotolerans is Cladosporium halotolerans 550W1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.41943. The invention further discloses a preparation method of the Cladosporium halotolerans. The application of the Cladosporium halotolerans in plant growth promotion belongs to the technical field of microorganisms. According to the salt-tolerant cladosporium sp. 550W1 provided by the invention, plant growth can be promoted by synthesizing auxin, gibberellin, cytokinin and other plant hormones; meanwhile, the salt-tolerant cladosporium sp. 550W1 has the outstanding advantages of being high in environmental adaptability, environmentally friendly, high in safety and the like, and an excellent core strain resource is provided for developing novel microbial fertilizers and promoting green sustainable agriculture.

The invention discloses an application of a CKX gene in regulating alfalfa root systems, comprising the following steps: step 1, preparing a transgenic wild-type alfalfa, overexpressing the MtCKX1 gene in the alfalfa, and obtaining a transgenic plant; the cDNA sequence and amino acid sequence of the MtCKX1 gene are sequence 1 and sequence 2 in the sequence table; step 2, comparing the root systems of the transgenic alfalfa plant with those of the wild-type plant, and the root systems of the transgenic alfalfa plant are larger than those of the wild-type plant; the cytokinin content of the transgenic plant is suppressed, and the root systems of the transgenic plant are strengthened and enlarged; cytokinin has a negative regulatory effect on the root growth of the plant, and after the MtCKX1 gene is overexpressed, the cytokinin is suppressed, and the root system of the plant is strengthened and enlarged, thereby effectively fixing the soil and preventing soil loss.

This invention discloses a composition for prolonging the flowering period of durian and a method for regulating the flowering period of durian. The composition includes: a first spray agent, consisting of the following components in the indicated weight ratios: 5-20g of cytokinin-like substances; 1-5g of gibberellin-like substances; 10-30g of polyamine-like substances; 5-15g of boron source; 100-300g of energy-regulating components; 1-5g of slow-release carrier and penetration-promoting components; and water to 1000g; and a second spray agent, consisting of the following components in the indicated weight ratios: 20-50g of flowering rhythm-regulating components, which are composed of 5-10g of paclobutrazol, 3-8g of uniconazole, and 0.5-2g of chlorpyrifos; 2-5g of slow-release carrier and penetration-promoting components; and water to 1000g. This invention, through the combined design of the first spray agent (flowering promoter) and the second spray agent (flowering control), transforms the initiation of durian flower buds from a "single trigger" to a "controllable, phased trigger," thereby prolonging the flowering period while maintaining or even improving fruit-setting capacity.

The embodiment of the invention provides a golden camellia suspension cell culture method which comprises the following steps: golden camellia leaves are collected and inoculated into a WPM liquid culture medium containing hormone for culture, primary callus is formed through induction, and the hormone comprises naphthylacetic acid and 6-benayl aminopurine; and inoculating the primary callus into a WPM liquid culture medium containing auxin and cytokinin, and culturing to obtain the golden camellia suspension cells.

The invention discloses a malania oleifera seed germination accelerating method and application thereof, and the method comprises the following steps: (1) applying mechanical force to malania oleifera seeds, so as to generate cracks on seed shells of malania oleifera to conduct the inner space of the seed shells and the outer part of the seed shells; the crack longitudinally extends on the seed shell and penetrates through the top of the seed shell; (2) soaking the seeds treated in the step (1) in an aqueous solution containing a dormancy breaking agent; the dormancy relieving agent comprises gibberellin and a cytokinin analogue; and (3) sowing the seeds treated in the step (2) in a breathable and water-containing culture medium to perform stratification and germination acceleration, and performing temperature control and regular irrigation in the period until the seeds germinate. According to the application, the method is applied to germination acceleration of malania oleifera seeds collected from wild malania oleifera plants in Guangxi Zhuang Autonomous Region and / or Yunnan.

The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS / WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

The invention provides a high-yield cultivation and planting management method for raspberry tea, and relates to the technical field of raspberry tea planting, the high-yield cultivation and planting management method for the raspberry tea mainly adopts urea, plant cytokinin, a raspberry tea extract, 1, 8-cineole and polyglutamic acid as nutritional agent components for foliage spraying; brassin, aspirin and Huabao No.1 are adopted as root soaking liquid, and root (branch) soaking treatment is conducted on the raspberry tea seedlings before transplanting or cuttage; conventional topdressing is performed after transplanting and field planting, and a nutritional agent is sprayed on leaf surfaces in time after leaf picking. According to the method, the defects in the prior art are overcome, rooting, germination and vine growth of the raspberry tea can be effectively promoted, the shoot tip and stem leaf yield of the raspberry tea is improved, and the economic benefit of planting the raspberry tea is comprehensively improved.

The invention provides an efficient asexual propagation method of ficus pandurata suitable for simple condition application. The method comprises the following steps: (1) explant disinfection: soaking a stem with an axillary bud in a composite disinfectant containing mercuric chloride; (2) primary culture: inoculating the disinfected stem into a starting culture medium containing cytokinin and auxin for culture; (3) multiplication culture: transferring the callus formed by primary culture to a differential culture medium to induce cluster buds; (4) carrying out vector rooting, namely cutting robust buds obtained by multiplication culture, and inserting the robust buds into a rooting culture medium containing microencapsulated auxin; and (5) transplanting and hardening seedlings: treating the tissue culture seedlings with a protective solution, and transplanting the treated tissue culture seedlings to a compound matrix. The method can realize substantive transformation from a specialized tissue culture technology to basic-level production, and solves the industrialization bottlenecks such as equipment threshold and operation complexity.

The present application relates to the field of plant growth regulator technology, and in particular, to a phenylurea compound, a method for preparing same, and use thereof, and a composition for promoting crop cell division and a method for using same. The phenylurea compound provided herein has cytokinin-like biological activity and can effectively promote the germination of crop seeds and the expansion of cotyledons, increase the chlorophyll content, and retain and enlarge the fruits, featuring a stable action effect, safety to crops, and the like. Experimental results show that the phenylurea compound of the present application can effectively promote the germination of rice and peanut seeds, expansion of radish and cucumber cotyledons, leaf pigment retention and yield increase of peanuts, fruit set and retention of zucchini, and enlargement and sweetness enhancement of grape fruits, etc., exhibiting cytokinin-like effects.

The invention relates to the technical field of plant cultivation, in particular to a tissue culture and plant regeneration method for bitter herbs. The method comprises the following steps: S1, preparing an explant: carrying out surface sterilization on seeds of the bitter bugleweed, inoculating the seeds into a first culture medium for culturing to obtain sterile seedlings, and selecting terminal buds or lateral buds of the sterile seedlings as the explant; s2, callus induction: inoculating the explant into a second culture medium for culture, and inducing to form a callus; s3, cluster bud induction and proliferation: inoculating the explant after the callus tissue is formed into a third culture medium for culture, and performing induced differentiation to form cluster buds; s4, rooting culture: separating the cluster buds obtained in the step S3, inoculating the cluster buds into a fourth culture medium for culture, and inducing rooting to form a complete plant. According to the application, auxin substances and cytokinin substances in a specific ratio are used in different stages, so that high efficiency and high stability of plant regeneration of the bitter herbs are guaranteed.

The invention relates to the technical field of plant biology, and discloses a lilium lancifolium virus-free seedling stem tip stripping culture method which sequentially comprises the following steps: (1) pretreatment: taking lilium lancifolium virus-carrying explants, carrying out first high-temperature stress treatment, then transferring into a sub-high-temperature condition, and carrying out second high-temperature stress treatment on the lilium lancifolium virus-carrying explants to obtain lilium lancifolium virus-free seedlings; carrying out shake culture by using a pretreatment culture medium containing plant stress resistance induction hormone; (2) stem tip stripping: after pretreatment in the step (1), stripping stem tip meristem with the size of 0.8-1.2 mm; (3) induction culture: the stem tip meristem stripped in the step (2) is inoculated to a solid induction culture medium for dark culture until callus is formed, and the solid induction culture medium contains cytokinin and an antiviral compound; through deep synergy of gradient temperature stress (high temperature to sub-high temperature) and specific stress-resistant hormone (BR) induction in time sequence and physiological functions, the physiological state of the explant in the pretreatment process is integrally regulated and controlled.

The invention discloses two plant growth regulation products for increasing the yield of carya illinoensis, and a preparation method and an application method of the two products. The first plant growth regulation product is prepared from 0.5 to 1.5 mmol / L of putrescine, 0.05 to 0.15 mmol / L of spermidine and 40 to 60 mg / L of cytokinin; and the second plant growth regulation product is prepared from 0.5 to 1.5 mmol / L of putrescine, 0.05 to 0.15 mmol / L of spermidine and 400 to 600 mg / L of cytokinin. The first plant growth regulation product and the second plant growth regulation product both can increase the number of female flowers, relieve the fruit drop condition in the development period and improve the fruit setting rate, and have positive influences on the yield increase of the carya illinoensis. The plant growth regulating product provided by the invention is simple in preparation method, simple in application method, easy to operate, high in practicability, capable of meeting the industrial development of large-scale cultivation and production of the carya illinoensis and wide in application prospect.

The invention discloses a method for promoting citral-type camphor tree clump bud proliferation, which comprises the following steps: selecting a stock plant which is free from plant diseases and insect pests and grows robustly, hanging a tag, and spraying cytokinin on leaf surfaces at set intervals from March; current-year semi-lignified branches are collected at the beginning of June; the stem segments are subjected to pruning and bacteriostatic soaking; performing multiplication culture, observing the growth condition of the cluster buds, and counting the multiplication coefficient and average plant height of the adventitious buds. According to the method, a large number of effective cluster buds are obtained in a short time through technologies such as stem segment bacteriostasis and germination promotion, culture medium and culture condition optimization and the like, the effective propagation coefficient of citral type camphor trees is increased, technical support is provided for conservation and propagation of tree species, the seedling cultivation period is shortened, the seedling cultivation generations are reduced, the seedling quality is guaranteed, and the method is suitable for large-scale popularization and application. And the waste of manpower and material resources is reduced, so that the healthy and sustainable development of citral camphor tree and essence and perfume industries is promoted.

The invention provides a phenylurea compound, a preparation method and application thereof, a composition for promoting crop cell division and a use method, and belongs to the technical field of plant growth regulators. The phenylurea compound disclosed by the invention has cytokinin-like biological activity, can effectively promote crop seed germination, cotyledon expansion, chlorophyll content increase, fruit retention and fruit swelling, and has the characteristics of stable action effect, safety to crops and the like. Experimental results show that the phenylurea compound has a cytokinin-like effect, and can effectively promote germination of rice and peanut seeds, enlargement of radish and cucumber cotyledons, green-keeping and yield-increasing of peanut leaves, fruit setting and retention of summer squashes, fruit swelling and sweetening of grapes and the like.

The invention relates to the technical field of gene engineering, in particular to a cotton gene GhAGL15 and application thereof. The GhAGL15 gene is identified in cotton, overexpression of GhAGL15 can significantly promote embryogenic callus induction of cotton, accelerate somatic embryo formation and improve the overall regeneration efficiency, hormone level determination finds that gibberellin in a GhAGL15 knockout strain is significantly accumulated, the cytokinin level in an overexpression strain is significantly increased, and the GhAGL15 knockout strain can be used as a GhAGL15 gene. It is prompted that GhAGL15 can play a role by regulating and controlling metabolism or signal pathways of gibberellin and cytokinin by regulating and controlling far-end regulation and control areas of related genes of gibberellin and cytokinin. And a basis is provided for breeding excellent cotton varieties.

This invention relates to compositions and methods for modifying Cytokinin Receptor Histidine Kinase (HK) genes in plants, optionally to improve yield traits, the HK genes encoding a histidine kinase (HK) polypeptide. The invention further relates to plants having increased improved yield traits produced using the methods and compositions of the invention.

The invention relates to a fruit tree shoot controlling and flower promoting composition and application thereof, and belongs to the technical field of fruit tree shoot controlling and flower promoting. The composition is mainly prepared from a ketoxime triazole compound and cytokinin, wherein the cytokinin is one of thidiazuron, 6-benayl aminopurine, 6-furfurylaminopurine and oxyenadenine. The treetop-controlling and flower-promoting composition for the fruit trees takes one of the ketoxime triazole compound and thidiazuron, 6-benayl aminopurine, 6-furfurylaminopurine and oxyenadenine as an active ingredient, is used as a leaf fertilizer, is convenient to spray, can better control the growth of the treetop of the fruit trees and promote the flower bud differentiation, and has the advantages of high yield, high quality and high yield. A foundation is laid for further improving the flowering rate and the yield of the fruit trees.

The present invention relates to methods of improving growth and yield in plants by applying an effective amount of a mixture of (S)-abscisic acid and one or more cytokinins to the plant. The present invention relates to methods of improving stress tolerance in plants by applying an effective amount of a mixture of (S)-abscisic acid and one or more cytokinins to the plant.

The invention relates to the technical field of fruit tree cross breeding, and discloses a method for obtaining seeds through artificial pollination of figs. The method comprises the following steps: treating Caprifig type figs by using a plant growth regulator to obtain pollen, and transferring the pollen into fig stigmas by using an artificial pollination dry powder method to obtain seeds. The method comprises the following steps: applying a cytokinin plant growth regulator at the stage that the fruit diameter of the Caprifig type figs is less than 3cm for treatment; harvesting mature Caprifig type fruits, separating anther, drying in the shade, and sieving to obtain pollen; fully mixing pollen and starch to form a pollen working agent in a dry powder state; at the stage that the diameter of the pollinated fig fruit is 1-3 cm, a pollen working agent is sprayed into a fruit cavity through a bract opening, so that the pollen working agent is attached to the stigma of a female flower; after the fruits are ripe, harvesting and water-selecting to obtain seeds. By means of the method, pollen with vitality can be obtained, artificial pollination and fruiting of the figs can be achieved, and the method is suitable for fig cross breeding and fruit improvement.

The application belongs to the technical field of plant genetic engineering, and particularly relates to a rice cytokinin response regulator gene OsRR11 and application. Research finds that OsRR11 encodes a conserved protein containing an aspartic acid signal receiving domain, and is a specific response regulator of cZ in rice; through research, it is found that OsRR11 has a positive regulation function in the resistance of rice to brown planthopper. Through agrobacterium-mediated genetic transformation, OsRR11 is overexpressed in japonica OsRR11 and is knocked out in Bph6-NIL, the resistance of the overexpression plant to brown planthopper is significantly enhanced, and the resistance of the knockout mutant plant to brown planthopper is significantly weakened. The gene of the application provides a good theoretical basis for studying how cytokinin participates in the insect resistance of rice through a response regulator, has a reference significance for studying the molecular function of a gene, and provides a gene resource for molecular design breeding of rice insect resistance.