691 results about "Cytokinin" patented technology

This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporal and / or spatial expression of genes that affect metabolically effective levels of cytokinins in plant seeds and related maternal tissue. This invention further relates to transgenic plants having enhanced levels of cytokinin expression wherein the transgenic plant exhibits useful characteristics, including: improved seed size, decreased tip kernel abortion, increased seed set during unfavorable environmental conditions, and stability of yield.

This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and / or spatially-regulated expression of genes that affect metabolically effective levels of cytokinins in plants, particularly in plant seeds and related female reproductive tissue. This invention further relates to transgenic plants having enhanced levels of cytokinin expression wherein the transgenic plant exhibits useful characteristics, such as improved seed size, decreased tip kernel abortion, increased seed set during unfavorable environmental conditions, or stability of yield. The present invention also provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions comprise novel nucleotide sequences for seed-preferred promoters known as eep1 and eep2. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.

The invention relates to 6-(alkylbenzylamino)purine derivatives of the general formula I for use as cytokinin receptor antagonists, wherein R1 is selected from the group comprising hydroxyl, amino, nitro, thio and alkyl group, and R2 denotes one to four alkyl groups. The invention also relates to preparations containing these derivatives.

The invention belongs to the field of plant growth regulators and application thereof, and in particular relates to a composition containing chitosan oligosaccharide and a plant growth regulator and application of the composition to promotion of growth and yield of plants. The plant growth regulator is composed of one or more of forchlorfenuron, diethyl aminoethyl hexanoate, compound sodium nitrophenolate, naphthylacetic acid, heteroauxin, polyamine, cytokinin and indolebutyric acid, wherein the weight ratio of the chitosan oligosaccharide to the plant growth regulator is 1:(0.01-50), and the application dosage of the composition is 0.05-10 ppm. The composition is applied to any plant required by humans directly or indirectly.

The invention discloses a biological salt-degrading bacterial fertilizer consisting of the following components by weight proportion: 0.0013 to 0.0045 portions of gibberellin, 0.00002 to 0.0002 portions of cytokinins, 15 to 35 portions of bacillus megaterium, 50 to 80 portions of salt-degrading flora, 5 to 15 portions of amino acid and 2 to 10 portions of humic acid. The materials are evenly mixed and air dried. The bacterial fertilizer is formed by being extracted from marine microorganisms, rejuvenation, expanding propagation and being remixed with a plurality of trace elements. The bacterial fertilizer is scientific in formulation and very strong in activity, can be widely applied to salt lick improvement and a secondary salinization treatment, improves and optimizes soil environment and improves the deteriorated soil environment caused by the long-term use of chemical fertilizers and salt containing organic fertilizers (dungs). The bacterial fertilizer can facilitate the development of the roots of crops and can improve the ability of absorbing night soil. By applying the biological salt-degrading bacterial fertilizer, the fertilizer can facilitate the even seedling, uniform seedling and strong seedling at the earlier stage of crops, the strong nutrient transmission capacity at the middle stage and no premature senescence at the later stage.

Soluble and stable liquid compositions containing a plant growth regulator selected from the group consisting of cytokinin and a gibberellin, an acid solubilizer such as citric acid, tartaric acid or glycolic acid and a solvent; as well as methods for making and using the composition are disclosed. The compositions improve solubility, handling, stability, safety, as well as activity improvements such as improved plant growth, yield, fruit thinning or sizing and quality. The compositions are soluble and stable by adding an ethoxylated alkyl alcohol wherein the growth regulator is 6-benzyladenine (6-BA) or forchlorfenuron (CPPU) and the ethoxylated alcohol surfactant is C12-15 alkyl alcohol in propylene glycol. The compositions may also contain a cytokinin such as 6-benzyladenine (6-BA) or forchlorfenuron (CPPU) that is increased in solubility and activity and by synergistically combined with GA3 or GA4A7 as well as in storage stability by adding an antioxidant. The compositions are formulated in a ready-to-mix formulation.

Water soluble plant growth regulating compositions in granule form and methods for making and using same. The granules comprise an active medium, a carrier medium and optionally a surfactant, wherein the active medium comprises: a gibberellin, a cytokinin, and an auxin. The inventive compositions are shelf stable and may be completely dissolved in water prior to application on a plant or seed.

Pharmaceutical compositions include compounds with cytokinin activity to modulate glucose and / or lipid metabolism in a mammal. Especially preferred compounds include those comprising a purine scaffold, and it is further preferred that contemplated compositions are employed to prevent and / or treat various diseases, including pre-diabetes, insulin resistance, type-2 diabetes, Syndrome X, and dyslipidemia In still further preferred aspects, compounds with cytokinin activity are used to activate AMPK and / or Akt. Consequently, various diseases associated with dysregulation of AMPK and / or Akt may be treated using the compounds of the present inventive subject matter.

The high efficiency blooming-promoting foliage spraying microbial fertilizer includes a mixture of amino acid of seven cytokinins, red sugar, potassium chlorate, mineral salt of nucleotide, ethylene,abscisic acid and potassium dihydrogen phosphate as main agent and a synergist comprising farm washing powder for foliage penetration, absorption and adhesion. It can promote blossom bud differentiation of fruit trees, especially longan, litchi, Shatian shaddock, and other substropical fruit trees. It has the features of low cost, high safety, high efficiency and no pollution.

The invention belongs to the field of regulation and control of plant growth, and particularly relates to a plant growth regulator composition for regulating and controlling the treetop growth of a fruit tree. The plant growth regulator composition comprises the following effective components used for regulating and controlling the plant growth: prohexadione calcium, compound sodium nitrophenolate / diethyl aminoethyl hexanoate / sodium naphthalene acetate / triacontanol / brassinolide / 6-benzyladenine; the plant growth regulator composition is diluted to the concentration of 150-800 mg / kg by being blended with water in terms of effective components to be used for regulating and controlling the growth of the fruit tree. Compared with the prior art, the plant growth regulator composition disclosed by the invention solves the problem that treetop control, flower promotion and safety yield increase are difficult to synchronize in a fruit tree treetop control process, induces the nutrient elements to be transmitted and concentrated to fruits by regulating the ratio of the growth hormone, cytokinin and gibberellin of the fruit tree during a growth period, enhances the flowering rate, enhances the fruiting rate, achieves the effects of inhibiting vegetative growth and promoting reproductive growth, is obvious in yield increase effect and outstandingly improves the quality of the fruits.

The present invention provides methods and DNA constructs for the genetic engineering of plant cells to produce plants which produce substantially seedless fruit in the absence of exogenous growth factors (auxins or cytokinins) and in the absence of pollination. The substantially seedless fruits produced by the methods described herein are about the size of wildtype seeded fruit (or somewhat larger) and these fruits are equal to or superior to the wildtype seeded fruit with respect to solid content and flavor. The seedless fruits of the present invention are produced in transgenic plants which contain and express auxin or cytokinin biosynthetic genes, e.g., tryptophan oxygenase or isopentenyl transferase coding sequences expressed under the regulatory control of GH3 or AGL promoter sequences directing preferential or tissue specific expression of a downstream gene in the ovaries or developing fruit.

The invention discloses a polygonatum cyrtonema seed rapid emerging method. Polygonatum cyrtonema fruits are harvested and washed after the fruits are mature to obtain clean seeds for cold sand storage immediately, dormancy is removed from hypocotyl after storage treatment of 120 days, the seeds are treated with gibberellin with proper concentration and soaking time and are not sprouted, and emerging doesn't occur in the current year; gibberellin treated seeds are sown in a seedling plate loaded with a loose, fertile and moist substrate, primary roots of polygonatum cyrtonema are grown fully after four months, dormancy is removed from epicotyl after the primary roots are taken out and treated with cytokinin 6-BA with proper concentration and soaking time, the seeds are transplanted in a shading seedling field, emerging starts after about 15 days, the emerging is uniform, and the emergence rate can be as high as 70%. The polygonatum cyrtonema seed rapid emerging method has the advantages that the seed germination rate is high after treatment, the emergence rate is high, and the seedling culture time is reduced by one year.

The invention discloses a balance growth regulator for a plant. The balance growth regulator is characterized by comprising a filling agent and at least four crop growth regulators selected from brassinolide, methyl jasmonate, auxin (heteroauxin), salicylic acid, cytokinin, gibberellic acid, abscisic acid, ethylene and triacontanol. The product disclosed by the invention is a plant growth regulator prepared from at least more than four traditional endogenous plant growth regulators, the balance growth regulator not only can be used for greatly increasing the yield, but also can be used for additionally producing dry matters, shows favorable crop disease and disadvantage resistance and is green, safe and incapable of injuring eaters.

The present invention provides a method for root fragment seedling cultivation of the Yunnan Manyleaf Paris Rhizome, which is to adopt the rhizome of the Yunnan Manyleaf Paris Rhizome to be fragmented in the period from March to April or in the period from September to October. The fragmented rhizome is immersed in a 2 to 10 mg / L Cytokinin 6-BA for 12 to 36 hours. The rhizome is laid indoor with one layer of soil. The thickness of the soil is 3 to 5 cm and the total stack height is 10 to 50 cm; the moisture content of the soil is kept at 50 percent to 70 percent; after the rhizome is sprouted to be one to three, the seedling is moved to a green house and is put on a seedling bed with a row distance of 2 to 5 to 2 to 5 cm in a high density; two years later, after the ramet of the seedling, the seedling can be moved to be cultivated in the farmland. Adopting the present invention, the loss rate of the root fragment material is reduced from 40 percent of the conventional farmland direct-planting fragment to 10 percent or less; the fragmented multi-sprout breeding rate is increased by 2 to 4 times; the general sprouting rate is increased by 3 to 5 times; 40 to 60 percent of the breeding material, the land and the management cost are saved with the present invention of integrated seedling culture and transplanted in the farmland compared to the traditional way of direct planting. The operability of the technology is strong and is easy for industrialization; the investment is less; the cost is low; the efficiency is high and the present invention has important realistic significance for effectively resolving the shortage of the seedling of Yunnan Manyleaf Paris Rhizome.

The invention relates to a rice anther culture improvement technology, and in particular relates to a method for improving the frequency of callus induction and plant differentiation rate in japonica rice anther culture. The method comprises the following steps that: 200g / 667m<2> of 10% of paclobutrazol wettable powder and 50-60L of water are mixed for spraying treatment before sampling, the spraying of the paclobutrazol and low-temperature treatment cause multiple pollen cells in the anther to be in the later monokaryotic stage, novel cytokinin 4-FA is introduced into a callus inducing medium, under the synergistic action of the 4-FA, KT, and 2,4-D, division is easily started in the dark culture after inoculation, and the frequency of callus induction is greatly improved. By using the anther culture method, the frequency of callus induction in the japonica rice anther culture is obviously improved to exceed 85.0%. A differential medium comprises the following ingredients: an MS (Murashige and Skoog) basic culture medium, 0.5mg / L of NAA, 0.5mg / L of ZTO, 0.8mg / L of TDZ, 0.2mg / L of FA, 0.2mg / L of MET, 30g / L of saccharose, and 8g / L of agar powder; and after the novel hormones of ZT, 4-FA and TDZ are introduced, light cultivation is carried out, and plant differentiation rate can reach more than 86%.

Treatment for Cancer Using A Preparation containing a combination of one or more Natural or Synthetic or a Combination of Natural and Synthetic Boswellic Acids and one or more Natural or Synthetic or a Combination of Natural and Synthetic Cytokinins such as Kinetin, Kinetin Riboside and other Cytokinins and (the Preparation) could also contain one or more Natural or Synthetic or a Combination of Natural and Synthetic plant growth hormones such as Auxins in any percentage Ratio and could Optionally include Pharmaceutically Suitable Radioactive Agent (Radio Therapy) in any percentage ratio as a “Stand Alone” Treatment for Cancer or to Be used in Conjunction with other Pharmaceutically Suitable Therapy and Treatment(s) in any percentage ratio as a Treatment for Treating Cancer.

The invention discloses a special growth regulating agent for jatropha and application thereof. The growth regulating agent is prepared according to the following steps: dissolving cytokinin into a sodium hydroxide solution; then, using distilled water for diluting the solution into mother liquid with the cytokinin concentration between 0.02 mg / mL and 100 mg / mL; and uniformly mixing 2 to 9999 parts of mother liquid, 1 to 10 parts of surface active agents and 0 to 9997 parts of distilled water to obtain the growth regulating agent. The growth regulating agent is uniformly sprayed on the jatropha whole strain until liquid drops drip down before or after 10 days of the occurrence of the blossom buds. After the growth regulating agent is sprayed on the jatropha plants for 1 to 3 times during each flowering period, the generated total flower number, the female flower proportion, the fruit number and the seed yield are respectively 3.6 times, 4.3 times, 4.5 times and 3.9 times of those of untreated jatropha. At the same time, the oil content of the seed is also improved from 31.67 percent to 34.76 percent, and is improved by about 9.8 percent. The invention has the advantages of simple and convenient operation, low cost and obvious economic benefit, and has good application prospects.

The present invention provides an approach to increase yield and vigor in a plant. The present invention describes expressing a heterologous gene in a plant that control cytokinin expression under the control of a cell cycle regulated promoter.

The invention discloses a Tilia tomentose seed presprouting method. The method comprises the following steps: selecting fully mature new seeds, picking off the seed vessel mechanically firstly, carrying out quick burning and immersion treatment, then immersing the seeds in gibberellin and cytokinin, and finally carrying out presprouting through a temperature changing lamination method adopting a warm temperature before a low temperature. The method brings about a short breeding cycle and a high sprouting rate of Tilia tomentose. The ideal sprouting rate can be achieved for about 23 days. Compared with the conventional presprouting method, the presprouting method greatly shortens the time needed by the presprouting of the seeds to achieve the aim of the rapid breeding of Tilia tomentose, and solves the problem of seed resource waste caused by the low seed sprouting rate of Tilia tomentose.

The invention provides a method for testing plant endogenous hormones, comprises the following steps of: carrying out microdialysis sampling on a plant body; carrying out sample injection on the acquired dialyzate on a high-performance liquid chromatograph; and simultaneously separating and determining four main plant endogenous hormones: gibberellic acid (GA), cytokinin (6-BA), indolylacetic acid (IAA) and abscisic acid (ABA). Compared with the traditional method for testing the plant endogenous hormones, the invention realizes the intravital test on the plant endogenous hormones and can reduce the damage to the plant body to maximum extent; in addition, the invention can simply and conveniently extract samples, has simple operation, can really reflect the change of the content of the endogenous hormones in the plant body in the vital movement progress and realize the real-time, intravital and on-line test on the plant endogenous hormones.

The invention provides a bacillus amyloliquefaciens ZCM18 with broad spectrum growth-promoting and stress-resisting effects. The preservation No. of the bacillus amyloliquefaciens ZCM18 is CGMCC No.12959. The bacterial strain has higher phosphate solubilization and nitrogen fixation capability and capability of generating IAA, ACCD, siderophore and cytokinin; the tolerance of salinity can reach up to 5%; the bacillus amyloliquefaciens has higher antagonism to fusarium and rhizoctonia pathogenic bacteria and is suitable for the production of multifunctional bio-fertilizer, such as, disease prevention, growth promoting and salt and alkali resistance; the application scope is wide; the bacillus amyloliquefaciens is suitable for biological improvement of various soil (saline-alkali soil, degenerated soil and conventional cultivated land) and production of various plants (vegetables, grain crops and fruit trees); the bacillus amyloliquefaciens has obvious effects in boosting the root development, preventing and controlling root diseases, increasing the crop yield, and the like; the bacillus amyloliquefaciens is capable of reducing the dosage of chemical fertilizers and pesticides and is wide in application prospect.

The invention provides an efficient transgenic method for maize skeleton inbred lines. According to the method, an efficient transgenic effect for the maize skeleton inbred lines is realized by adding 0.01-1mg / L of cytokinin KT(6-glycosylaminopurine) in a co-culture medium and a rest culture medium, and determining the optimal screening agent concentration in a selective culture medium, i.e., the adding concentration of balaphos is 0.5-50mg / L. The method provided by the invention has the characteristics of being short in experiment period, high in positive rate, and simple and convenient to operate, used for preparing transgenic maize, and suitable for large-scale popularization.

The invention provides a production method of a natural organic seaweed fertilizer. The method comprises the following steps: treating seaweeds by using a method of combining mechanical crushing centrifugation with chemical alkali digestion, washing the digested slurry material with water and performing filtration and concentration so as to obtain a corresponding semi-solid filter material; compounding the dried filter material; and producing the compounded filter material by using a granulating process so as to obtain the organic seaweed fertilizer. According to the method, a relatively small amount of alkali is added and an enzyme agent is not used, i.e., and the increasing of the additional cost is avoided, so that the requirement on the working environment of a reaction kettle is relatively low. The produced organic seaweed fertilizer contains a large quantity of organic substances without containing nitrogen and multiple mineral nutrients such as potassium, magnesium and zinc, and particularly contains seaweed polysaccharides and highly unsaturated fatty acids which are specifically contained in the seaweeds and is rich in multiple plant growth regulators such as cytokinins and auxins, thereby having the high biological activity. Thus, the absorption and the growth of plants are promoted by the beneficial substances from the seaweed plants. As a result, the stress resistance of crops is improved; the yield of the crops is increased.

The invention discloses compositions for the preservation of fruits, vegetables, partially processed products, other produce and followers. The compositions comprise at least one phospholipase D inhibitor, at least one compound comprising an isoprene subunit, at least one component of the flavonoid biosynthetic pathway in a suitable medium. The composition of the present invention may additionally comprise one or more plant growth regulators of the cytokinin type, one or more antioxidants, a membrane stabilizing agent, a surfactant, or any combination thereof. The composition may be applied to produce as a spray, drench, dip, or a vapour and at either the pre-harvest stage or post-harvest stage.

The invention discloses a tree nutrient solution, which comprises the following ingredients in percentage by weight: 20-25% of glucose, 10-15% of chitosan, 0.1-1% of cytokinin, 0.1-1% of polyamine, 0.01-0.1% of potassium hydroxide, 0.01-0.1% of ferric citrate, 0.01-0.1% of zinc sulfate, 0.01-0.15% of copper sulfate, 0.01-0.2% of ammonium molybdate, 0.03-0.1% of boric acid, 0.01-0.1% of cobalt chloride, 0.05-0.2% of vitamin C and the balance of water. The nutrient solution provided by the invention does not contain any harmful substance, and can not pollute the environment or cause damage to the human body after application; the preparation cost is low, the action time is long, and the nutrient solution can be widely used.

The purpose of the present invention is to disclose an in-bottle rooting method for a salt tolerance ulmus pumila tissue culture seedling. The method comprises the following steps: (1) selecting a secondary seedling, wherein a secondary culture salt tolerance ulmus pumila tissue culture seedling is selected, the height of the seedling is 2-3.5 cm, and the robust individual ulmus pumila seedling having extending leaves is selected; and (2) inoculating the selected secondary culture salt tolerance ulmus pumila tissue culture seedling onto a WPM rooting medium, wherein the WPM rooting medium is added with growth hormone, cytokinin and exogenous additives. According to the present invention, a medium formula having a high rooting rate is adopted so as to effectively solve the rooting problem of the salt tolerance ulmus pumila tissue culture seedling, improve the rooting rate of the salt tolerance ulmus pumila tissue culture seedling, reduce the cost, accelerate the factory production of the salt tolerance ulmus pumila, provide high quality salt tolerance ulmus pumila seedlings for nursery stock markets, and achieve the purpose of the present invention, wherein the rooting rate of the salt tolerance ulmus pumila tissue culture seedling can be up to 95%.

The invention discloses an agrobacterium tumefaciens gene transformation method of hybrid poplar. The method comprises the following steps of: (1) pre-culture; (2) strain activation and culture; (3) infection; (4) co-culture; (5) delayed selection; (5) adventitious bud induction; (7) extension culture; and (8) root induction and detection. In the method provided by the invention, NAA and 2ip act together to induce the agrobacterium tumefaciens-mediated stem section or leaf stalk to generate callus; under the effect of cytokinin TDZ, the callus redifferentiation is promoted to form adventitious buds; the bud in a vitrified state extends under the induction of BAP (Benzo A Pyrene), and the form gradually becomes normal; and the resistant regenerated poplar roots in a 1 / 2MS (Murashige and Skoog) culture medium containing antibiotic hygromycin. The resistant genetically-modified poplar obtained by the method has the advantages of short period and high transformation efficiency, thereby laying firm foundation and theoretical support for the practical breeding of the genetically-modified poplar.

Plant-growth-promoting bio-organic fertilizer is prepared by inoculating 0.5-2% (w / w) of composite fermentation microbial agent to livestock-poultry manure and auxiliary material, fermenting, inoculating 2-5% (w / w) of plant-growth-promoting microbial agent and fermenting. A preparation method includes steps: (1), preparing a fermenting material; (2), performing high-temperature aerobic fermentation; (3), preparing the plant-growth-promoting microbial agent; (4), after-composting organic fertilizer. The plant-growth-promoting bio-organic fertilizer and the preparation method have the advantages that rapid proliferation of plant-growth-promoting functional microorganisms is realized in the process of fermenting of two times, and effective biological viable bacteria count of the organic fertilizer reaches 5x108CFU / g; composting degree is high, the organic fertilizer can generate various plant hormones including 3-indoleacetic acid, cytokinin, gibberellins and ethylene after being applied in soil, can promote plant growth and can increase yield of crops and incoming of farmers; the bio-organic fertilizer prepared by the method is low in cost, environment-friendly and high in efficiency.

The present disclosure belongs to the technical field of plant genetic engineering, and discloses a gene for controlling the rice grain number per panicle and its use. Nucleotide sequence of OsCKX11 is SEQ ID NO. 1, nucleotide sequence for coding protein region is SEQ ID NO. 2, amino acid sequence of the encoded protein is SEQ ID NO. 3. The disclosure constructs an OsCKX11-knocked-out vector using CRISPR / Cas9, and identifies multiple independent homozygous lines through PCR amplification and sequencing methods, and provides a mutant in which specific knockout of gene OsCKX11 of the rice leads to an increase in cytokinin levels and an increase in grain number per panicle. Based on the biological function of OsCKX11 to increase the rice grain number per panicle, methods such as gene editing, natural allele replacement, RNA interference, or molecular assisted breeding can be used to improve existing rice varieties.