Bacillus amyloliquefaciens with broad spectrum growth-promoting and stress-resisting effects

A technology for dissolving amyloid spores and bacilli, applied in microorganism-based methods, chemicals for biological control, bacteria, etc., can solve problems such as increased development costs, inconvenience in practical application, and limited application scope, and achieve improved yield and quality. , improve drought tolerance, reduce the effect of chemical fertilizers and pesticides

Active Publication Date: 2017-04-26
INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In nature, due to the selective pressure of the ecological environment, such as salinity, drought, etc., there are many plants that tolerate or adapt to these extreme environments and the microorganisms that live with them. The limit of general biological growth and survival, but the growth of energy Microorganisms adapted to this extreme environment, there are a large number of microorganisms in the soil, plant rhizosphere or plant tissue, among which the strains that have the functions of dise...

Method used

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  • Bacillus amyloliquefaciens with broad spectrum growth-promoting and stress-resisting effects
  • Bacillus amyloliquefaciens with broad spectrum growth-promoting and stress-resisting effects

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Isolation and identification of Bacillus amyloliquefaciens ZCM18

[0026] 1. Isolation of strains

[0027] Take 1 g of wheat rhizosphere soil from saline-alkali land in Haixing County and put it into 10 mL of sterile saline, and shake it with a vortex instrument for 1 min to prepare a rhizosphere soil bacterial suspension. The bacterial suspension was serially diluted, spread on LB plates, and cultured in a 30°C incubator for 48 hours to isolate bacterial strains.

[0028] 2. Morphological characteristics of the strain

[0029] Form round, flat, moist and viscous colonies on LB plate, grow turbidly in LB liquid medium, and form bacterial film in the later stage of culture. After Gram staining, it can be seen under the microscope that the ends are blunt and round, of different lengths, Gram-positive bacilli, and there are subterminal spores that are larger than the bacteria.

[0030] 3. Physiological and biochemical characteristics of the strain

[0031] Ex...

Embodiment 2

[0036] Embodiment 2 Bacillus amyloliquefaciens ZCM18 biological fertilizer preparation

[0037] The seed medium of Bacillus amyloliquefaciens ZCM18 is LB medium, inoculate the bacterium solution stored in glycerol tubes on LB solid medium for activation, culture at 35°C for 2 days, and inoculate a single colony into LB liquid medium, at 35°C, 200r / min, culture 15h to the late logarithmic period, and the bacterial liquid obtained after the inoculation amount of 1% is transferred once more is the seed liquid. Fermentation of ZCM18 was carried out with this seed solution. At 35°C, 200r / min, cultured for 48 hours, the number of bacteria can reach 1×10 10 cfu / mL.

[0038] The seed medium of Bacillus amyloliquefaciens ZCM18 is LB medium, inoculate the bacterium solution stored in glycerol tubes on LB solid medium for activation, culture at 35°C for 2 days, and inoculate a single colony into LB liquid medium, at 35°C, 200r / min, culture 15h to the late logarithmic period, and the b...

Embodiment 3

[0039] Embodiment 3 bacillus amyloliquefaciens ZCM18 anti-fusarium, rhizoctonia test

[0040] Take the soil from the surface of the vegetable field to 20cm above, air-dry, crush, sieve, and autoclave as the basic cultivation substrate. Carry out pot test with the ZCM18 powder biological fertilizer that embodiment 2 makes. In the same way, the Bacillus amyloliquefaciens WW814 strain bio-fertilizer was obtained. This strain was screened out before and developed into a microbial fertilizer. Registration number: Microbial Fertilizer (2016) Zhunzi (1915). The pathogenic Fusarium (Fusarium.sp) and Rhizoctonia (Rhizoctonia.sp) are activated and inoculated on the high-pressure sterilized bran culture, and cultivated at 25° C. for 7-10 days. The bran culture covered with pathogenic mycelium is air-dried and crushed.

[0041] Preparation of cultivation substrates for different treatments. Treatment 1: Sterilized soil: ZCM18 biological fertilizer: pathogenic inoculum=8.5:1:0.5 (volume...

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Abstract

The invention provides a bacillus amyloliquefaciens ZCM18 with broad spectrum growth-promoting and stress-resisting effects. The preservation No. of the bacillus amyloliquefaciens ZCM18 is CGMCC No.12959. The bacterial strain has higher phosphate solubilization and nitrogen fixation capability and capability of generating IAA, ACCD, siderophore and cytokinin; the tolerance of salinity can reach up to 5%; the bacillus amyloliquefaciens has higher antagonism to fusarium and rhizoctonia pathogenic bacteria and is suitable for the production of multifunctional bio-fertilizer, such as, disease prevention, growth promoting and salt and alkali resistance; the application scope is wide; the bacillus amyloliquefaciens is suitable for biological improvement of various soil (saline-alkali soil, degenerated soil and conventional cultivated land) and production of various plants (vegetables, grain crops and fruit trees); the bacillus amyloliquefaciens has obvious effects in boosting the root development, preventing and controlling root diseases, increasing the crop yield, and the like; the bacillus amyloliquefaciens is capable of reducing the dosage of chemical fertilizers and pesticides and is wide in application prospect.

Description

technical field [0001] The invention relates to the field of soil biological improvement, in particular to a strain of Bacillus amyloliquefaciens with broad-spectrum growth-promoting and stress-resistance effects and its application. Background technique [0002] Due to global warming, unreasonable farming (such as continuous cropping), improper use of chemical fertilizers and other factors, soil salinization, biological barriers and other problems are becoming more and more serious, which not only further deteriorates soil quality and ecological environment, but also has become an obstacle to crop production. The main factors of healthy growth and high yield and high quality. In addition, there are still large areas of land resources such as coastal beaches and inland saline-alkali land that need to be improved and utilized. At present, the main ways to solve the problem are disease-resistant breeding and chemical pesticides to control diseases, crop rotation and stubble pr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P3/00C05F11/08C12R1/07
CPCA01N63/00C05F11/08C12N1/20C12N1/205C12R2001/07
Inventor 王占武彭杰丽张翠绵胡栋贾楠黄晓东
Owner INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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