Bacillus amyloliquefaciens with broad spectrum growth-promoting and stress-resisting effects
A technology for dissolving amyloid spores and bacilli, applied in microorganism-based methods, chemicals for biological control, bacteria, etc., can solve problems such as increased development costs, inconvenience in practical application, and limited application scope, and achieve improved yield and quality. , improve drought tolerance, reduce the effect of chemical fertilizers and pesticides
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Embodiment 1
[0025] Example 1 Isolation and identification of Bacillus amyloliquefaciens ZCM18
[0026] 1. Isolation of strains
[0027] Take 1 g of wheat rhizosphere soil from saline-alkali land in Haixing County and put it into 10 mL of sterile saline, and shake it with a vortex instrument for 1 min to prepare a rhizosphere soil bacterial suspension. The bacterial suspension was serially diluted, spread on LB plates, and cultured in a 30°C incubator for 48 hours to isolate bacterial strains.
[0028] 2. Morphological characteristics of the strain
[0029] Form round, flat, moist and viscous colonies on LB plate, grow turbidly in LB liquid medium, and form bacterial film in the later stage of culture. After Gram staining, it can be seen under the microscope that the ends are blunt and round, of different lengths, Gram-positive bacilli, and there are subterminal spores that are larger than the bacteria.
[0030] 3. Physiological and biochemical characteristics of the strain
[0031] Ex...
Embodiment 2
[0036] Embodiment 2 Bacillus amyloliquefaciens ZCM18 biological fertilizer preparation
[0037] The seed medium of Bacillus amyloliquefaciens ZCM18 is LB medium, inoculate the bacterium solution stored in glycerol tubes on LB solid medium for activation, culture at 35°C for 2 days, and inoculate a single colony into LB liquid medium, at 35°C, 200r / min, culture 15h to the late logarithmic period, and the bacterial liquid obtained after the inoculation amount of 1% is transferred once more is the seed liquid. Fermentation of ZCM18 was carried out with this seed solution. At 35°C, 200r / min, cultured for 48 hours, the number of bacteria can reach 1×10 10 cfu / mL.
[0038] The seed medium of Bacillus amyloliquefaciens ZCM18 is LB medium, inoculate the bacterium solution stored in glycerol tubes on LB solid medium for activation, culture at 35°C for 2 days, and inoculate a single colony into LB liquid medium, at 35°C, 200r / min, culture 15h to the late logarithmic period, and the b...
Embodiment 3
[0039] Embodiment 3 bacillus amyloliquefaciens ZCM18 anti-fusarium, rhizoctonia test
[0040] Take the soil from the surface of the vegetable field to 20cm above, air-dry, crush, sieve, and autoclave as the basic cultivation substrate. Carry out pot test with the ZCM18 powder biological fertilizer that embodiment 2 makes. In the same way, the Bacillus amyloliquefaciens WW814 strain bio-fertilizer was obtained. This strain was screened out before and developed into a microbial fertilizer. Registration number: Microbial Fertilizer (2016) Zhunzi (1915). The pathogenic Fusarium (Fusarium.sp) and Rhizoctonia (Rhizoctonia.sp) are activated and inoculated on the high-pressure sterilized bran culture, and cultivated at 25° C. for 7-10 days. The bran culture covered with pathogenic mycelium is air-dried and crushed.
[0041] Preparation of cultivation substrates for different treatments. Treatment 1: Sterilized soil: ZCM18 biological fertilizer: pathogenic inoculum=8.5:1:0.5 (volume...
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