128results about How to "Improve salt tolerance" patented technology

The invention relates to a ring structure betaine and double-tailed hydrophobic association polymer fracturing thickening agent and a preparation method thereof. The thickening agent is formed by copolymerization of four monomers, namely acrylamide, 2-allyl methylamino sodium ethanesulfonate, methyl diallyl sulfoethyl betaine and hydrophobic monomer double-tailed long-chain alkyl methyl allyl ammonium chloride. The preparation method comprises the following steps: firstly adding distilled water, then sequentially adding acrylamide, 2-allyl methylamino sodium ethanesulfonate, methyl diallyl sulfoethyl betaine and a hydrophobic monomer double-tailed long-chain alkyl methyl allyl ammonium chloride, adjusting the total concentration, introducing N2 to remove oxygen, adding an initiator, and reacting to prepare an AM / AMES / MDASBA / DCnMAAC quadripolymer, namely the polymer fracturing thickening agent. The thickening agent provided by the invention has good water solubility, and shows good salt resistance in mixed brine with the mineralization degree of greater than or equal to 30000 mg / L. The prepared linear solution and gel formed after crosslinking have high viscosity retention capacity at the temperature of 150 DEG C and the shearing rate of 170 s <-1 >, and the actual construction requirement for fracturing yield increase with the on-site mineralization degree larger than or equal to 30000 mg / L is met.

The invention provides a novel strain M2108 of a salt-tolerant bacillus, the preservation number of the salt-tolerant bacillus is CGMCC (China General Microbiological Culture Collection Center) No.28283, and the novel strain M2108 of the salt-tolerant bacillus has relatively good salt tolerance and can grow and proliferate in a high-salt environment; the strain can be rapidly and stably colonized in a plant, and is rapid in propagation speed and strong in multiplication capacity in the plant. The new strain M2108 of the salt-tolerant bacillus provided by the invention has a treatment effect and a high control effect on various plant diseases: the control effect on diseases such as tomato root rot, damping-off and damping-off caused by fusarium oxysporum, pythium aphanidermatum and rhizoctonia solani is high, and the average control effect is 91.2% or above. When being used for preventing and treating plant diseases, the salt-tolerant bacillus M2108 is not easy to generate drug resistance, has good drug effect durability, is safe to human and livestock, and does not cause environmental pollution; the salt-tolerant bacillus M2108 microbial agent and the biocontrol agent provided by the invention are simple in preparation method, low in cost and simple and convenient to use. The salt-tolerant bacillus M2108 has a good application prospect in the prevention and control of plant diseases, and lays a foundation for the development of efficient and broad-spectrum biocontrol preparations.

The invention relates to a rice OsABCB7 gene as well as an encoding protein and application thereof. The inventor utilizes a CRISPR / Cas9 gene editing system to obtain two homozygous knockout mutants osabcb7-1 and osabcb7-9 of an OsABCB7 gene, wherein the two homozygous knockout mutants osabcb7-1 and osabcb7-9 take japonica rice Nipponbare as a background and have different editing conditions. Many-year and multi-generation agronomic trait investigation shows that under the normal field planting condition, the grain length, grain width, thousand seed weight, single plant yield and the like in the mature period are all remarkably increased compared with those of a wild type contrast, and the maturing rate has no obvious difference; however, under the continuous high-temperature condition, the maturing rate and the yield of a single plant of the mutant are obviously higher than those of a wild type control. Besides, the mutant rice after salt treatment shows enhanced salt tolerance, so that the OsABCB7 gene can be applied to rice crossbreeding and molecular design breeding to improve the rice yield and enhance the salt tolerance and high temperature resistance of the rice.

The invention discloses an OsFBX421 gene and application of the OsFBX421 gene in the aspect of improving the salt tolerance of rice. The nucleotide sequence of the OsFBX421 gene is as shown in SEQ ID NO: 1 in a sequence table. The invention further discloses a phenotype of an OsFBX421 gene mutant strain under treatment of 120 mM NaCl, and the protein and the coding gene thereof have important theoretical and practical significance on research of a plant salt-tolerant mechanism and improvement of salt tolerance and related traits of plants, can play an important role in plant salt-tolerant molecular breeding and have wide application prospects.

The invention relates to the technical field of agricultural biology, in particular to application of a BnaC04.bZIP16 gene to improvement of salt tolerance of plants. The sequence of the BnaC04.bZIP16 gene provided by the invention is as shown in SEQ ID NO.1, and the coded protein sequence of the BnaC04.bZIP16 gene is as shown in SEQ ID NO.2. The invention discovers that the BnaC04.bZIP16 gene can be highly expressed after wild brassica napus is irrigated with saline water, and meanwhile, the transgenic brassica napus with the over-expressed gene can better grow in a salt stress environment, so that the BnaC04.bZIP16 gene can be used for preparing a salt-tolerant brassica napus. The rape with the BnaC04.bZIP16 gene knocked out is more difficult to grow in a salt stress environment, so that the effect of the BnaC04.bZIP16 gene as a forward regulatory factor in enhancing the salt tolerance of the rape is verified, and a key molecular tool is provided for developing a new variety of brassica napus with high salt tolerance; the gene has wide application potential and commercial value in the field of agricultural planting, especially in improving the salt tolerance of crops and cultivating transgenic crops with high salt tolerance.

The invention relates to aramid nanofiber-based carbonaceous aerogel and a preparation method thereof. The preparation method comprises the following steps: dispersing aramid fibers in an alkali solution, and washing with deionized water to obtain an aramid fiber dispersion liquid; performing vacuum filtration on the aramid fiber dispersion liquid, and performing freeze drying to obtain aramid fiber nanofiber aerogel; the aramid fiber nanofiber aerogel is subjected to carbonization treatment, and the aramid fiber nanofiber based carbonaceous aerogel is prepared. According to the preparation method disclosed by the invention, the highest evaporation rate of the obtained aramid nanofiber-based carbonaceous aerogel can reach 3.42 Kg / m < 2 > h by regulating and controlling the hydrolysis degree of the aramid fibers and combining vacuum suction filtration, and the aramid nanofiber-based carbonaceous aerogel has a good evaporation effect; in actual use, hydrophobic treatment is carried out on the upper surface of aerogel, and the defect that the photo-thermal evaporation effect of a product is reduced due to the fact that an open water layer is generated at a gas-liquid interface in the use process is effectively overcome.

The invention provides a tracer agent for an oil field and a method for evaluating oil production contribution of each section of a horizontal well, and belongs to the technical field of oil field development. The tracer agent for the oil field, provided by the invention, is prepared from white oil, a nonionic surfactant, 3-allyloxy-2-hydroxy propanesulfonic acid sodium salt and alcohols; wherein the rare earth metal salt, the complexing agent and the sodium 3-allyloxy-2-hydroxy propanesulfonate form a complex; the complexing agent is a mixture of glycolic acid, triethylene tetramine and sodium dimercaptopropanesulfonate in a mass ratio of 10: (3-5): (1-2). The tracer agent for the oil field can stably exist under complex stratum conditions and can resist hypersalinity; in the process of evaluating the oil production contribution of each section of the horizontal well by using the tracer agent for the oil field, different types and different dosages of tracer agents are selected for different layer sections, so that the evaluation of the oil production contribution of each section of the horizontal well can be realized, and an effective basis is provided for subsequent refracturing.

The invention discloses application of a wheat TaSSRP1 protein and a coding gene thereof in regulation and control of plant salt tolerance. According to the invention, DNA molecules for coding TaSSRP1 protein are respectively introduced into wild type arabidopsis thaliana and wild type wheat, so that TaSSRP1 overexpressed arabidopsis thaliana and TaSSRP1 overexpressed wheat are respectively obtained. Experiments prove that under salt stress, compared with wild type arabidopsis thaliana and wild type wheat, the salt tolerance of TaSSRP1 overexpression arabidopsis thaliana and the salt tolerance of TaSSRP1 overexpression wheat are both improved, and it is indicated that the salt stress tolerance of plants is improved through TaSSRP1 overexpression. The invention discovers that the TaSSRP1 protein and the coding gene thereof have the function of regulating and controlling the salt tolerance of the plant for the first time, and the TaSSRP1 protein and the coding gene thereof have an important value for cultivating salt-tolerant plant varieties.

The invention provides a graphene oxide composite oil-displacing agent and a preparation method and application thereof.The graphene oxide composite oil-displacing agent is prepared from poly (3-hydroxytyramine) and graphene oxide, the poly (3-hydroxytyramine) and the graphene oxide are combined through chemical bonds, the preparation method comprises the steps that hydrochloric acid 3-hydroxytyramine and the graphene oxide are subjected to a mixed reaction, and the graphene oxide composite oil-displacing agent is obtained. The obtained composite oil-displacing agent is applied to tertiary oil recovery. The composite oil displacement agent provided by the invention has excellent hydrophilicity, acid and alkali resistance and salt resistance, can form a stable water-in-oil emulsion with crude oil in a wide pH value range or under high salinity, and can improve the stability and viscosity of the emulsion; the emulsifying effect is strong, the emulsifying swept volume can be enlarged, and the application prospect in medium-low permeability oil reservoirs is wide; the preparation method provided by the invention is simple, convenient and high in practicability.

The invention discloses an ultra-thick solvent-free epoxy coating for an offshore steel pipe pile as well as a preparation method and application of the ultra-thick solvent-free epoxy coating. The ultra-thick solvent-free epoxy coating is prepared from a component A and a component B, the component A comprises the following components in percentage by mass: 20-25% of epoxy resin, 20-25% of silicon branched modified epoxy resin, 2-5% of a reactive diluent, 10-20% of calcium carbonate, 10-20% of natural barium sulfate, 2-5% of graphene, 10-15% of glass flakes, 5-10% of silica powder, 5-10% of zinc phosphate, 0.8-2% of a thixotropic agent and 0.2-0.3% of a flatting agent; and the component B comprises 15-30% of a curing agent, 15-25% of talcum powder, 15-25% of natural barium sulfate, 10-15% of titanium dioxide, 5-10% of zinc phosphate and 0.2-0.3% of a leveling agent. According to the present invention, the adhesion, the salt resistance, the wear resistance and the aging resistance of the epoxy coating can be improved, and the epoxy coating can be used for the anti-corrosion coating of the offshore steel pipe pile close to or in contact with seawater.

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS2 Dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA1300 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS2 It is constructed from genes. SsNHX1 Genes and SsSOS2 The CDS coding sequence of the gene is shown in SEQ ID NO:1 and SEQ ID NO:2 of the sequence listing. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantage lies in the synergistic effect of the two genes, which effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

The invention belongs to the technical field of cosmetics, and particularly relates to a cosmetic delivery system based on a natural wall material, a preparation method and application. The cosmetic delivery system is of a core-shell structure, a core is a cosmetic active component, and a shell layer is a composite polymer gel network of sodium alginate, sophorolipid and cellulose. The natural polysaccharide composite wall material is green and safe, has good biocompatibility and strong compatibility with cosmetic formulas, and does not irritate the skin. The delivery system can effectively protect cosmetic active ingredients from being damaged by illumination and oxygen, and the storage stability of the active ingredients is remarkably improved; meanwhile, slow release and targeted transdermal absorption of active ingredients are realized, and the bioavailability is improved.

The invention discloses a synthetic functional bacterial agent and application of the synthetic functional bacterial agent in salt tolerance and growth promotion of wheat, the synthetic functional bacterial agent is prepared from five strains, namely, Gordonia hydrophora, Lysinibacillus oldseyi, planomicrobium okeanokites, Luteimonas huaabiensis and Brevibacterium epidermidis, and the bacterial agent has the remarkable characteristics of no antagonism among the strains, salt and alkali tolerance, high viable count and the like, and can be applied to salt tolerance and growth promotion of wheat. The germination rate, the seedling length and the root length of the spring wheat can be remarkably increased, and the seedling length and the root length of the winter wheat are also remarkably promoted. Under the condition of salt stress, the microbial inoculum can remarkably improve the activity of catalase, peroxidase and superoxide dismutase of wheat and the total content of chlorophyll, after microbial inoculum treatment, the content of malondialdehyde and proline of wheat is remarkably reduced, and the microbial inoculum plays a role in promoting and protecting spring wheat and winter wheat in the process of resisting saline-alkali stress and has a good application prospect. The method has an influence effect on agricultural development and sustainable development in the arid region.

The invention discloses a slow-release bacterial fertilizer as well as a preparation method and application thereof, and relates to the technical field of biotechnology. Comprising the following steps: step 1, preparing a bacterial liquid of bacillus cereus, a bacterial liquid of lysinibacillus slender, a bacterial liquid of acinetobacter schiffei, a bacterial liquid of stenotrophomonas maltophilia, a bacterial liquid of sphingobacterium alcohovorans and a bacterial liquid of bacillus licheniformis into a mixed bacterial suspension according to a volume ratio of 1: (1-2): (1-2): (1-2): (1-2): (1-2); 2, mixing the mixed bacterium suspension with an embedding agent and a cross-linking agent to obtain a first mixed colloid; step 3, embedding the first mixed colloid into the second mixed layer to obtain first compound fertilizer particles; and step 4, embedding the first compound fertilizer particles into a fermented third mixed layer to obtain the slow-release bacterial fertilizer. The prepared slow-release bacterial fertilizer improves the remediation effect of organic phosphorus contaminated soil. The soil fertility is improved, and a good growth promoting effect on plants is achieved.

The invention discloses an OsEIE3 gene and application of the OsEIE3 gene in improving the salt tolerance of rice. The nucleotide sequence of the OsEIE3 gene is shown as SEQ ID NO: 1 in a sequence table. The invention also discloses an application of the OsEIE3 gene mutant plant in improving the salt tolerance of the plant. The protein and the coding gene thereof have important theoretical and practical significance in research of a plant salt tolerance mechanism, improvement of salt tolerance of plants and improvement of related traits, can play an important role in plant salt tolerance gene engineering improvement, and have wide application prospects.

The invention discloses a method for in-situ enrichment of anaerobic ammonia oxidizing bacteria, a bio-membrane reactor is adopted, and a carrier of a bio-membrane is made of a material with good conductivity and biological affinity; the direct-current power supply continuously outputs weak voltage below 1.0 V; preparing special wastewater as inlet water of the reactor; sludge containing anaerobic ammonium oxidation bacteria is selected as inoculation sludge, the hydraulic retention time of the reactor is controlled, microorganisms in the reactor mainly grow on a carrier in an attached mode to form a biological membrane, and suspended sludge is eliminated as much as possible; aeration is performed on the reactor, and water enters and exits the reactor continuously. According to the method, the anaerobic ammonium oxidation bacteria are enriched under the micro-aerobic condition, the nitrogen source is only ammonia nitrogen, nitrite nitrogen is generated in the ammonia nitrogen conversion process instead of being supplied externally, the normal form of anaerobic ammonium oxidation enrichment culture is innovated through the method, and the anaerobic ammonium oxidation enrichment culture efficiency is improved. The problem that anaerobic ammonia oxidizing bacteria cannot be enriched due to lack of external supply of nitrite nitrogen in the actual wastewater treatment process is effectively solved.

The invention discloses a salt-tolerant grease degrading bacterium, a biological agent and application of the biological agent in grease degradation, and belongs to the technical field of biodegradation. The strain name of the salt-tolerant grease degrading bacterium is IURM G72, and the salt-tolerant grease degrading bacterium is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.29726. The salt-tolerant grease degrading bacterium IURM G72 and the preparation thereof have good salt tolerance and grease degradation performance, can grow by taking grease as a unique carbon source under the condition of relatively high salt concentration, and have simple nutritional requirements. The degrading bacterium and the preparation thereof can effectively degrade grease in high-salt kitchen waste, and have important significance on water bodies, soil and the like polluted by waste edible grease.

The invention relates to a special single-coating type heat vulcanization adhesive for bonding butyl rubber and metal and a preparation method thereof, and the special single-coating type heat vulcanization adhesive comprises liquid rubber, a vulcanizing agent, diphenylmethane-4, 4 '-diisocyanate MDI, epoxy modified phenolic resin, pigment carbon black, fumed silica, titanium dioxide, zinc oxide and an organic solvent. According to the single-coating type heat vulcanization adhesive, the bonding strength of butyl rubber and metal is greatly improved, the bonding performance is excellent, the bonding strength is obviously higher than that of a heat vulcanization adhesive of metal and an elastomer material, the high temperature resistance and salt resistance of a bonding interface are excellent, the peel strength is still kept to be 11 kN / m or above in a salt spray experiment and a boiling water experiment, and the service life of the adhesive is prolonged. The environment resistance such as wear resistance, corrosion resistance and heat resistance is excellent.

The invention relates to the field of polymer materials, and discloses a super absorbent fiber for a core layer material of a diaper. The super absorbent fiber comprises the following raw materials in parts by weight: 70 to 90 parts of sodium acrylate solution, 20 to 45 parts of polyacrylamide microspheres, 2 to 10 parts of modified hydroxyethyl methacrylate, 10 to 30 parts of polyvinyl alcohol solution, 0.5 to 5 parts of an initiator, and 1 to 5 parts of a crosslinking agent; the polyacrylamide microspheres are prepared by an inverse suspension polymerization method; the modified hydroxyethyl methacrylate is hydroxyethyl methacrylate grafted with oxidized multi-walled carbon nanotubes to modify hydroxyethyl methacrylate. The invention improves the water absorption rate, salt resistance and fracture toughness of the super absorbent fiber by adding polyacrylamide microspheres and modified hydroxyethyl methacrylate.

The invention relates to the field of oilfield chemistry, and discloses a graphene oxide and polymer composite filtrate reducer for water-based drilling fluid and a preparation method of the graphene oxide and polymer composite filtrate reducer. The method specifically comprises the following steps: (1) adding graphene oxide powder into distilled water, stirring and performing ultrasonic treatment to obtain a graphene oxide aqueous solution for later use; (2) sequentially adding functional monomers, namely 2-acrylamide-2-methyl-1-propanesulfonic acid, 4-acryloylmorpholine and N, N-dimethylacrylamide into the graphene oxide aqueous solution for later use, and uniformly stirring; (3) adding ammonium persulfate and sodium hydrogen sulfite, heating in a water bath to a temperature required by reaction, and introducing nitrogen to wait for reaction; and (4) after the reaction is finished, washing with ethanol, drying, and crushing to obtain the graphene oxide and polymer composite filtrate reducer for the water-based drilling fluid. According to the invention, graphene oxide sheets are utilized to cover pores and polymer chains are utilized to connect bentonite particles, so that the water-based drilling fluid containing the filtrate reducer can resist the temperature of 220 DEG C, can resist salt by 15%, and can be applied to drilling of deep wells and ultra-deep wells.

The invention discloses a rose salt sensitive site gene RrSPL9 and application thereof, and belongs to the technical field of plant genetic engineering, the nucleotide sequence of the gene RrSPL9 is as shown in SEQ ID NO.1, and the protein amino acid sequence of the gene RrSPL9 is as shown in SEQ ID NO.2. The RrSPL9 reduces the salt tolerance of transgenic roses and arabidopsis thaliana, the miRNA resistance target RrSPL9mut transgenic phenotype is more prominent, which indicates that the RrSPL9 gene is a key salt-sensitive site, the RrSPL9 gene is expected to greatly enhance the salt tolerance of roses after being knocked out or silenced, and the RrSPL9 gene has important application value in the field of salt-tolerant stress-resistant breeding.

The invention discloses a preparation method of organic acid modified biochar as well as a product and application of the organic acid modified biochar, reference and a method are provided for resource utilization of a large number of waste grape branches, salicylic acid modified grape-based biochar obtained by modifying salicylic acid with the concentration of 50 mg / L and 500 mg / L has excellent specific surface area; therefore, excellent adsorption performance is obtained. According to the invention, the grape-based biochar obtained by modification with 500 mg / L of salicylic acid can improve the salt resistance level of eggplants under the stress of 100 mmol / L of NaCl. The invention provides a new method for efficient resource utilization of the rape straws. The rape straw biochar obtained by modifying the 100 mg / L ferulic acid can obviously improve the fresh weight of lettuce.

The invention relates to the technical field of oil field chemicals, and discloses a hydrolysis-resistant organosilicon nonionic surfactant and a preparation method thereof. The problems that in the prior art, due to retention of fracturing fluid in the flowback process, the stratum permeability is reduced, and the oil and gas development effect is affected are solved. The technical scheme adopted by the invention is as follows: weighing 10-15 parts of acrylic acid; 60 to 70 parts of octylphenol polyoxyethylene ether; the preparation method comprises the following steps: uniformly mixing the following components in parts by weight: 20-30 parts of sodium hydroxide, 0.06-0.30 part of hydroquinone and 0.6-1.8 parts of p Introducing nitrogen and heating for reaction; adding 0.05% dichloromethane for dissolving, adjusting the solution to be neutral by using a sodium hydroxide solution, and carrying out reduced pressure distillation at 45 DEG C; after the reaction is finished, adding 5 parts of isopropanol and 0.005 part of chloroplatinic acid hexahydrate, uniformly mixing, introducing nitrogen, heating for half an hour, dropwise adding heptamethyltrisiloxane at a constant speed, controlling the reaction temperature at 80-120 DEG C, and reacting for 5-7 hours to obtain brownish yellow viscous liquid; and carrying out reduced pressure distillation on the brownish yellow viscous liquid at 45 DEG C.

The invention discloses a nano black phosphorus thick oil viscosity reducer as well as a preparation method and application thereof. The nano black phosphorus thick oil viscosity reducer is prepared from the following raw materials: oil-soluble polymer modified molybdenum disulfide nanosheets, black phosphorus nanosheets and a coupling agent, wherein the mass ratio of the oil-soluble polymer modified molybdenum disulfide nanosheets to the black phosphorus nanosheets to the coupling agent is 1: 2: (2-6). The nano black phosphorus thick oil viscosity reducer has the advantages of strong acting force with asphaltene, high viscosity reduction rate, good temperature resistance and salt resistance, good compatibility with crude oil and difficult aggregation.

This invention relates to the field of genetic engineering technology, specifically disclosing the application of the SlCHP16 gene and its overexpression vector in tomato cultivation. The cDNA sequence of the SlCHP16 gene is shown in SEQ ID No. 1. Transforming tomato plants with the overexpression vector constructed using the SlCHP16 gene significantly promotes tomato root growth and significantly enhances the tomato's resistance to salt and alkali stress. The SlCHP16 gene overexpression vector provided by this invention is beneficial for promoting tomato root growth and enhancing the tomato's resistance to salt and alkali stress.

The purpose of this invention is to disclose a rice salt tolerance-related gene ZOS2-02, its encoded protein, and its applications. The gene ZOS2-02 is a DNA molecule as described in either 1) or 2) below: 1) a DNA molecule with a genomic sequence as shown in SEQ ID NO.1; 2) a DNA molecule with a CDS sequence as shown in SEQ ID NO.2; 3) a DNA molecule that hybridizes to the DNA sequence defined in 1) or 2) under stringent conditions and encodes the protein. The genetic engineering application of the gene ZOS2-02 provided by this invention in regulating rice salt tolerance specifically involves knocking out the aforementioned gene ZOS2-02 to improve rice salt tolerance.

The application relates to a gold nanoparticle array preparation method with strong stability and reusability, high cysteamine-stabilized gold nanoparticles are prepared first, the spacing between the gold nanoparticles is changed by utilizing the coulomb repulsion between the gold nanoparticles, then the gold nanoparticle solution is added dropwise to a glass surface modified by gamma-glycidoxypropyltrimethoxysilane to react, stable covalent bonds are formed, and gold nanoparticle arrays with uniform distribution on the glass surface can be obtained after the reaction. The preparation does not require high temperature, the experimental operation is simple and convenient, the gold nanoparticles are arranged in order and closely, have high detection sensitivity, have strong ultraviolet absorption capacity, good stability, reusability, salt resistance and low background interference, and can be reused. The gold nanoparticle array can realize detection analysis on high-salt-content samples in surface-assisted laser desorption ionization mass spectrometry and be used for rapid analysis of small molecule compounds in positive and negative ion dual modes.

The invention relates to the technical field of genetic engineering breeding, in particular to application of ZmC2DP1 protein in regulation and control of drought resistance and salt tolerance of plants, the amino acid sequence of the ZmC2DP1 protein is as shown in SEQ ID NO.1, and the nucleotide sequence of a gene for coding the ZmC2DP1 protein is as shown in SEQ ID NO.2. After the ZmC2DP1 gene is knocked out, the drought resistance and the salt resistance of a mutant plant under the drought treatment or salt treatment condition are improved compared with those of a control group plant, and it is verified that the ZmC2DP1 protein has a negative regulation function on the drought resistance and the salt resistance of the plant. Compared with a traditional breeding mode, the method for regulating and controlling the drought resistance and the salt resistance of the plants has the advantages that the breeding time is short, the purposiveness is high, the drought resistance breeding period is remarkably shortened, and the crop stress resistance breeding efficiency is improved; a gene editing homozygous strain with improved drought resistance and salt tolerance is obtained by adopting a gene knockout technology, and gene resources are provided for cultivating and improving new varieties of stress-resistant plants; and a theoretical basis is provided for clarification of a molecular mechanism of the ZmC2DP1 protein in drought resistance and salt resistance of plants.

This invention discloses a water-soluble anti-collapse agent, its preparation method, and a water-based slurry. The raw materials for the water-soluble anti-collapse agent include melamine, formaldehyde aqueous solution, polyol, epichlorohydrin, water, initiator, organophosphonate, and comonomer. The preparation method includes the following steps: mixing melamine and formaldehyde aqueous solution, adjusting the pH, and then reacting; introducing polyol into the reaction system to continue the reaction; then introducing organophosphonate solution into the reaction system, followed by epichlorohydrin to continue the reaction; finally introducing comonomer and initiator to continue the reaction, and then drying and optionally crushing to obtain the water-soluble anti-collapse agent. A water-based slurry containing the above-mentioned water-soluble anti-collapse agent is also provided. The anti-collapse agent provided by this invention has a filtration loss reduction rate of more than 70% against complex salts and a shale secondary rolling recovery rate of more than 80%. It has good salt resistance, temperature resistance, wall consolidation, and filtration loss reduction performance, and also has the advantages of being environmentally friendly, having low fluorescence, and good water solubility.

The invention discloses application of a sweet potato IbbHLH149 gene in improvement of plant stress resistance, and belongs to the technical field of molecular biology. The nucleotide sequence of the sweet potato IbbHLH149 gene is as shown in SEQ ID NO.1, and the coded amino acid sequence of the sweet potato IbbHLH149 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing a plant expression vector of the IbbHLH149 gene, and transforming arabidopsis thaliana; the result shows that the morphological development of the arabidopsis thaliana plant into which the IbbHLH149 gene is transferred is normal, and the arabidopsis thaliana seedling into which the IbbHLH149 gene is transferred can at least resist the stress of 125mM NaCl high salt or the stress of 400mM mannitol; the expression of the IbbHLH149 gene in arabidopsis thaliana can significantly improve the salt tolerance and drought resistance of the arabidopsis thaliana. Therefore, the IbbHLH149 gene has an important application value in the cultivation of stress-resistant plants.