14results about How to "Improve salt tolerance" patented technology

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS2 Dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA1300 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS2 It is constructed from genes. SsNHX1 Genes and SsSOS2 The CDS coding sequence of the gene is shown in SEQ ID NO:1 and SEQ ID NO:2 of the sequence listing. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantage lies in the synergistic effect of the two genes, which effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

The application relates to a gold nanoparticle array preparation method with strong stability and reusability, high cysteamine-stabilized gold nanoparticles are prepared first, the spacing between the gold nanoparticles is changed by utilizing the coulomb repulsion between the gold nanoparticles, then the gold nanoparticle solution is added dropwise to a glass surface modified by gamma-glycidoxypropyltrimethoxysilane to react, stable covalent bonds are formed, and gold nanoparticle arrays with uniform distribution on the glass surface can be obtained after the reaction. The preparation does not require high temperature, the experimental operation is simple and convenient, the gold nanoparticles are arranged in order and closely, have high detection sensitivity, have strong ultraviolet absorption capacity, good stability, reusability, salt resistance and low background interference, and can be reused. The gold nanoparticle array can realize detection analysis on high-salt-content samples in surface-assisted laser desorption ionization mass spectrometry and be used for rapid analysis of small molecule compounds in positive and negative ion dual modes.

The application discloses a salt-resistant water-non-dispersible material for oil well cement and a preparation method thereof. The salt-resistant water-non-dispersible material for oil well cement comprises the following components in the form of weight parts: 100 parts of N,N-dimethyl acrylamide, 3-6 parts of acrylic acid, 10-20 parts of 2-acrylamide-2-methylpropanesulfonic acid, 5-15 parts of a cyclic monomer, 0.1-0.4 parts of a branching agent, 5-15 parts of a cationic monomer, 0.3-3 parts of a crosslinking agent and 400-500 parts of water. The salt-resistant water-non-dispersible material prepared by the application has good salt resistance, can significantly improve the anti-dispersion performance of the cement slurry when used in a salt water and sea water cement slurry system with a NaCl content of 18 wt% or less, and has excellent rheological performance.

This invention relates to the field of oilfield development technology, and discloses a polymer with viscosity-reducing function, its preparation method, and its application. The polymer has the structural units shown in Formulas 1 and 2. Oil displacement agents containing this polymer exhibit high static viscosity reduction, high oil recovery, salt resistance, and high-temperature resistance.

This invention relates to the field of plant genetic engineering, specifically to the LbMYB75 gene of *Limonium bicolor* and its applications. This invention discloses that the transcription factor LbMYB75 is specifically highly expressed in the early stages of salt gland development and is significantly induced by salt. This invention confirms that the LbMYB75 gene positively regulates salt gland development and salt secretion in *Limonium bicolor*. Salt gland density is significantly increased in *Limonium bicolor* lines overexpressing the LbMYB75 gene, while salt gland density is significantly decreased in LbMYB75 mutant lines. The transcription factor LbMYB75 positively regulates the salt tolerance of *Limonium bicolor*; compared with the wild type, the overexpressing lines under high salt conditions show higher levels of malondialdehyde (MDA) and sodium. + and Na + / K + K decreased significantly. + Increased proline; This invention elucidates the regulatory role of the LbMYB75 transcription factor in salt gland development of *Limonium bicolor*, providing genetic resources and theoretical basis for improving the salt tolerance of non-halophytes through transgenic means, and contributing to the development and utilization of saline-alkali soils.

The application discloses a composite aerogel and a preparation method and application thereof, and belongs to the technical field of functional materials and environmental treatment. In the application, delignified cotton stalk cellulose is compounded with MXene to prepare a suspension, directional freezing self-assembly is carried out by using n-heptane as a heat transfer medium, and after freezing drying and polydimethylsiloxane dip coating, a PDMS / CMNF / MXene composite aerogel is obtained. The aerogel has an ultra-fine scale, high-density vertically arranged pore channels and a multi-level pore structure, and the pore channel diameter is 20-40 mu m. Under the driving of solar energy, the surface temperature of the aerogel can reach above 120 DEG C, the rapid in-situ viscosity reduction and efficient adsorption of high-viscosity crude oil are realized, the adsorption capacity is more than 56 times of the weight of the aerogel, and the performance retention rate is greater than 90% after 10 cycles. The application provides an efficient, green and recyclable solution for the treatment of marine high-viscosity crude oil leakage.

This invention belongs to the field of genetic engineering technology, specifically involving TaSCAMP17 The application of its recombinant overexpression vector in improving plant salt tolerance, the TaSCAMP17 The nucleotide sequence is shown in SEQ ID NO.1, and the amino acid sequence is shown in SEQ ID NO.2. This invention will... TaSCAMP17 Functional validation was performed in Arabidopsis thaliana after overexpression, and the results showed that overexpression... TaSCAMP17 The salt tolerance of wild-type Arabidopsis thaliana is significantly improved, therefore TaSCAMP17 It plays a positive regulatory role in wheat salt tolerance, providing a theoretical basis and gene resources for wheat molecular breeding, and is of great significance for obtaining salt-tolerant and high-yielding lines and improving wheat salt tolerance in the future.

The application provides a binary ionic liquid fiber membrane, a preparation method and application in adsorption of palladium, and belongs to the technical field of adsorption materials, and comprises the following steps: mixing dichloromethane and acetic acid to obtain a first solution; dissolving cellulose acetate, a first ionic liquid and a second ionic liquid in the first solution to obtain a second solution; the first ionic liquid and the second ionic liquid are alkenyl imidazole salts with different length alkyl side chain molecular structures; adding N,N'-methylene bisacrylamide and benzoyl peroxide into the second solution, heating, stirring and warming to obtain a third solution; performing high-pressure electrospinning on the third solution to obtain a binary ionic liquid fiber membrane; or performing irradiation on the second solution after high-pressure electrospinning to obtain a binary ionic liquid fiber membrane. The binary ionic liquid fiber membrane prepared by the application can efficiently and selectively adsorb and recover palladium from a complex acidic solution, and has the advantages of faster adsorption speed, better selectivity and better salt resistance.

This invention proposes a porous foam with a biomimetic lotus seedpod surface structure and its preparation method, belonging to the technical field of photothermal conversion materials, to solve the technical problems of poor water evaporation performance and salt resistance in solar evaporators. This invention proposes a composite foam prepared using a combination of vacuum solution casting technology / salt template method / template-assisted technology, which simultaneously possesses a hemispherical pore structure on the surface and a honeycomb-like hierarchical pore structure in the lower layer. Benefiting from the unique surface and internal pore structure, the solar evaporator exhibits highly efficient and stable evaporation performance in NaCl solutions of different concentrations, high salt resistance, and good self-cleaning ability. This double-layer hierarchical porous composite foam has broad application prospects in wastewater treatment and seawater desalination. The preparation method has advantages such as simple process, convenient operation, controllable surface pore structure and internal pore morphology, and the ability to form evaporators with complex shapes.

This invention belongs to the field of genetic engineering technology, specifically involving SGR Application of gene mutants and their biological products in salt-tolerant high-yield rice. SGR Gene mutants are SGR It is obtained by inserting a single base A or T between the 42nd and 43rd nucleotides of the gene coding sequence; SGR The gene coding sequence is shown in SEQ ID NO.1; compared with wild-type rice, SGR Genetic mutant rice exhibits high salt tolerance and high yield. SGR Rice prepared from bioproducts related to gene mutants exhibits higher salt tolerance and higher yield, thus improving breeding efficiency.

The application discloses GhSZF3 Application of a gene in improving plant salt tolerance, and belongs to the technical field of cotton salt tolerance gene research. GhSZF3 The gene can improve plant salt tolerance, and can significantly improve the seed germination ability of transgenic Arabidopsis under salt stress, which has important significance for cultivating new transgenic plant varieties with salt stress resistance. The gene can provide a wider gene source for directional editing, improvement of cotton salt resistance and molecular breeding of various plants and crops.

This invention provides the application and method of Streptomyces S16-1 in promoting the growth and salt stress tolerance of Leymus chinensis, belonging to the field of microbial technology. Streptomyces S16-1 was isolated from saline soil, with the preservation number CCTCC M 20251940, and its 16S rDNA sequence is shown in SEQ ID NO:1. The Streptomyces S16-1 has functional activities such as salt and alkali tolerance, phosphorus solubilization, nitrogen fixation, and production of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. The microbial fertilizer is obtained by expanding the culture of Streptomyces S16-1. Compared with chemical fertilizers, the microbial fertilizer used in this invention is more environmentally friendly. It can not only improve soil fertility and help plants improve their salt and alkali resistance, but also promote the balance and diversity of soil microbial communities, which helps maintain the health and stability of the soil ecosystem.

The application relates to the technical field of fire extinguishing agents, and particularly discloses a foam fire extinguishing agent compounded by anionic Gemini surfactants and a preparation method thereof, which comprises the following components in percentage by mass: 5-15% of anionic Gemini surfactants; 0.5-3% of fluorocarbon surfactants; 3-9% of hydrophilic monomer oligomers; 2-5% of zwitterionic surfactants; 1.0-2.5% of nonionic surfactants; 3-12% of anti-burning agents; 2-7% of cosolvents; and the balance of water. The biodegradable short-chain fluorocarbon surfactants are precisely compounded with anionic Gemini surfactants of specific structures, hydrophilic monomer oligomers and the like, and a preparation process of step-by-step construction is adopted, so that the environmental friendliness is remarkably improved, the performance of the fire extinguishing agent is comprehensively broken through, the water film on the oil surface is ensured to be rapidly spread and rapidly extinguished, the foam covering layer is endowed with super-strong mechanical stability and thermal stability, the anti-reignition time is greatly prolonged, and the purposes of efficient fire extinguishing, long-lasting anti-burning and environmental friendliness are finally achieved.

This invention relates to the field of microbial inoculant preparation technology, specifically to a thermophilic and halophilic *Trichoderma* agent and its preparation method. The active ingredient of the *Trichoderma* MT-ST / GP agent in this invention is *Trichoderma* MT-ST / GP, deposited at the China General Microbiological Culture Collection Center (CGMCC), accession number CGMCC No. 41587, and the agent contains 8 × 10⁸ viable cells. 6 ~1.2×10 7 The cellulase activity is 45-55 U / mL, the hemicellulase activity is 75-85 U / mL, and the salt tolerance concentration is 4-20 g / L NaCl. This invention improves the number of viable bacteria, enzyme activity, and salt tolerance of the inoculant by optimizing fermentation conditions (segmented temperature control, nutrient supplementation, and protective agent adaptation). It is suitable for scenarios such as agricultural waste degradation and biological treatment of high-salt environments.