Patents
Literature
Patsnap Eureka AI that helps you search prior art, draft patents, and assess FTO risks, powered by patent and scientific literature data.

379results about How to "Improve salt tolerance" patented technology

Cyclic structure betaine and double-tailed hydrophobic association polymer fracturing thickening agent and preparation method thereof

The invention relates to a ring structure betaine and double-tailed hydrophobic association polymer fracturing thickening agent and a preparation method thereof. The thickening agent is formed by copolymerization of four monomers, namely acrylamide, 2-allyl methylamino sodium ethanesulfonate, methyl diallyl sulfoethyl betaine and hydrophobic monomer double-tailed long-chain alkyl methyl allyl ammonium chloride. The preparation method comprises the following steps: firstly adding distilled water, then sequentially adding acrylamide, 2-allyl methylamino sodium ethanesulfonate, methyl diallyl sulfoethyl betaine and a hydrophobic monomer double-tailed long-chain alkyl methyl allyl ammonium chloride, adjusting the total concentration, introducing N2 to remove oxygen, adding an initiator, and reacting to prepare an AM / AMES / MDASBA / DCnMAAC quadripolymer, namely the polymer fracturing thickening agent. The thickening agent provided by the invention has good water solubility, and shows good salt resistance in mixed brine with the mineralization degree of greater than or equal to 30000 mg / L. The prepared linear solution and gel formed after crosslinking have high viscosity retention capacity at the temperature of 150 DEG C and the shearing rate of 170 s <-1 >, and the actual construction requirement for fracturing yield increase with the on-site mineralization degree larger than or equal to 30000 mg / L is met.
Owner:HEBEI PETROLEUM VOCATIONAL & TECH UNIV

Hydrogel thin layer-foam-based solar evaporator and preparation method and application thereof

The invention discloses a hydrogel thin layer-foam-based solar evaporator and a preparation method and application thereof, and belongs to the field of water treatment. The invention provides a hydrogel thin layer-foam-based solar evaporator. The hydrogel thin layer-foam-based solar evaporator comprises a foam substrate, a photo-thermal layer and a hydrogel thin layer, a photo-thermal layer and a hydrogel thin layer are sequentially coated on the foam substrate, or the photo-thermal layer, the hydrogel thin layer and the photo-thermal layer are sequentially coated on the foam substrate. The photo-thermal layer is made of a carbon-based indium material and is obtained by mixing an indium nitrate solution and a trimesic acid solution, preparing a precursor through a hydrothermal method and calcining the precursor in a protective atmosphere. The hydrogel thin layer-foam-based solar evaporator has efficient evaporation rate and energy utilization efficiency, and under irradiation of one sun, the FCH evaporation rate and the energy efficiency are 7.93 kgm <-2 > h <-1 > and 315% respectively; in addition, the compound also shows excellent salt resistance in 3.5-20wt.% of saline water, and also has a relatively high evaporation rate in acid-base and various dye sewage.
Owner:SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI

Bacillus velezensis as well as separation method and application thereof

The invention relates to the technical field of agricultural microbial agents, in particular to bacillus velezensis as well as a separation method and application thereof. The bacillus velezensis is preserved in the China General Microbiological Culture Collection Center, the preservation date is May 17, 2024, the preservation number is CGMCC No.30687, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the postcode is 100101, and the bacillus velezensis is classified and named as bacillus velezensis. The bacillus velezensis separated from the soil of the Ningboo garden of the Academy of Agricultural Sciences in Yitai City of Shandong Province or the fermentation inoculant thereof can be simultaneously used as a preparation raw material of a growth promoting agent, a yield increasing agent, a soil conditioner and a bio-organic fertilizer, and has multiple application scenes.
Owner:YANTAI ACAD OF AGRI SCI SHANDONG PROVINCE (YANTAI BRANCH OF SHANDONG ACAD OF AGRI SCI)

Tomato root rot biocontrol bacterium bacillus halotolerans M2108 and application of tomato root rot biocontrol bacterium bacillus halotolerans M2108

The invention provides a novel strain M2108 of a salt-tolerant bacillus, the preservation number of the salt-tolerant bacillus is CGMCC (China General Microbiological Culture Collection Center) No.28283, and the novel strain M2108 of the salt-tolerant bacillus has relatively good salt tolerance and can grow and proliferate in a high-salt environment; the strain can be rapidly and stably colonized in a plant, and is rapid in propagation speed and strong in multiplication capacity in the plant. The new strain M2108 of the salt-tolerant bacillus provided by the invention has a treatment effect and a high control effect on various plant diseases: the control effect on diseases such as tomato root rot, damping-off and damping-off caused by fusarium oxysporum, pythium aphanidermatum and rhizoctonia solani is high, and the average control effect is 91.2% or above. When being used for preventing and treating plant diseases, the salt-tolerant bacillus M2108 is not easy to generate drug resistance, has good drug effect durability, is safe to human and livestock, and does not cause environmental pollution; the salt-tolerant bacillus M2108 microbial agent and the biocontrol agent provided by the invention are simple in preparation method, low in cost and simple and convenient to use. The salt-tolerant bacillus M2108 has a good application prospect in the prevention and control of plant diseases, and lays a foundation for the development of efficient and broad-spectrum biocontrol preparations.
Owner:XINJIANG ACAD OF AGRI SCI (XINJIANG BRANCH OF CHINESE ACAD OF AGRI SCI)

Rice OsABCB7 gene as well as encoding protein and application thereof

The invention relates to a rice OsABCB7 gene as well as an encoding protein and application thereof. The inventor utilizes a CRISPR / Cas9 gene editing system to obtain two homozygous knockout mutants osabcb7-1 and osabcb7-9 of an OsABCB7 gene, wherein the two homozygous knockout mutants osabcb7-1 and osabcb7-9 take japonica rice Nipponbare as a background and have different editing conditions. Many-year and multi-generation agronomic trait investigation shows that under the normal field planting condition, the grain length, grain width, thousand seed weight, single plant yield and the like in the mature period are all remarkably increased compared with those of a wild type contrast, and the maturing rate has no obvious difference; however, under the continuous high-temperature condition, the maturing rate and the yield of a single plant of the mutant are obviously higher than those of a wild type control. Besides, the mutant rice after salt treatment shows enhanced salt tolerance, so that the OsABCB7 gene can be applied to rice crossbreeding and molecular design breeding to improve the rice yield and enhance the salt tolerance and high temperature resistance of the rice.
Owner:ZHEJIANG FORESTRY UNIVERSITY

OsFBX421 gene and application thereof in salt tolerance of rice

The invention discloses an OsFBX421 gene and application of the OsFBX421 gene in the aspect of improving the salt tolerance of rice. The nucleotide sequence of the OsFBX421 gene is as shown in SEQ ID NO: 1 in a sequence table. The invention further discloses a phenotype of an OsFBX421 gene mutant strain under treatment of 120 mM NaCl, and the protein and the coding gene thereof have important theoretical and practical significance on research of a plant salt-tolerant mechanism and improvement of salt tolerance and related traits of plants, can play an important role in plant salt-tolerant molecular breeding and have wide application prospects.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI

Application of BnaC04.bZIP16 gene in improving salt tolerance of plants

The invention relates to the technical field of agricultural biology, in particular to application of a BnaC04.bZIP16 gene to improvement of salt tolerance of plants. The sequence of the BnaC04.bZIP16 gene provided by the invention is as shown in SEQ ID NO.1, and the coded protein sequence of the BnaC04.bZIP16 gene is as shown in SEQ ID NO.2. The invention discovers that the BnaC04.bZIP16 gene can be highly expressed after wild brassica napus is irrigated with saline water, and meanwhile, the transgenic brassica napus with the over-expressed gene can better grow in a salt stress environment, so that the BnaC04.bZIP16 gene can be used for preparing a salt-tolerant brassica napus. The rape with the BnaC04.bZIP16 gene knocked out is more difficult to grow in a salt stress environment, so that the effect of the BnaC04.bZIP16 gene as a forward regulatory factor in enhancing the salt tolerance of the rape is verified, and a key molecular tool is provided for developing a new variety of brassica napus with high salt tolerance; the gene has wide application potential and commercial value in the field of agricultural planting, especially in improving the salt tolerance of crops and cultivating transgenic crops with high salt tolerance.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI

Self-suspending proppant for low-water fracturing

ActiveCN119307250AImprove salt tolerancelow water quality requirementsFluid removalDrilling compositionMeth-Active agent
The invention relates to the technical field of drilling compositions, in particular to a self-suspending proppant for low-water fracturing. The invention provides a self-suspending proppant for less-water fracturing. The self-suspending proppant comprises a modified proppant and a quadripolymer adsorbed on the surface of the modified proppant, the modified proppant is obtained by carrying out surface modification on a proppant through a modifier, and the modifier is a silane coupling agent and / or a nonionic surfactant; comonomers of the quadripolymer are acrylamide, 2-acrylamide-2-methylpropanesulfonic acid, 2-methacryloyloxyethyl trimethyl ammonium chloride and acrylic acid respectively. The four comonomers are adopted to form a quadripolymer, so that the salt resistance of the self-suspending proppant can be greatly improved, and the self-suspending proppant can still keep better suspending performance in a solution with higher mineralization degree and has better low-temperature gel breaking performance.
Owner:CHENGDU SINAIJI PETROLEUM TECH SERVICE CO LTD

Mildew-proof modified starch-based super absorbent resin as well as preparation method and application thereof

The invention belongs to the technical field of materials, and discloses mildew-proof modified starch-based super absorbent resin as well as a preparation method and application thereof. The mildew-proof modified starch-based super absorbent resin is prepared from the following raw materials in parts by mass: 10 to 20 parts of modified carboxymethyl starch, 2 to 3 parts of quaternized carboxymethyl chitosan, 10 to 15 parts of 2-acrylamido-2-methylpropanesulfonic acid, 15 to 25 parts of acrylamide, 100 parts of acrylic acid, 0.4 to 0.7 part of a redox initiator and 0.05 to 0.15 part of a cross-linking agent. According to the invention, the inherent defects of low mechanical strength, poor salt tolerance, poor stability, easy decomposition, short service life and the like of the starch-based resin are obviously improved, so that the starch-based super absorbent resin with good mildew resistance and more excellent performance can be developed.
Owner:GUANGDONG UNIV OF TECH +1

Candida tropicalis with effect of reducing nitrite and ammonia nitrogen and application of candida tropicalis

PendingCN119120235AStrong ability to degrade nitriteStrong ability to degrade ammonia nitrogenFungiWater contaminantsBiotechnologyNitrogen accumulation
The invention discloses candida tropicalis with the effect of reducing nitrite and ammonia nitrogen and application of the candida tropicalis. The saccharomycetes are respectively named as candida tropicalis JM-2, and the preservation number of the candida tropicalis JM-2 is GDMCC No: 64449. The saccharomyces cerevisiae is high in adaptability, grows well in high-concentration ammonia nitrogen and nitrite environments, is relatively high in nitrogen degradation efficiency, can be used as a functional strain for improving water quality, deodorizing farms, deodorizing garbage yards and the like, can be applied to a plurality of places, effectively degrades harmful substances such as nitrite, ammonia nitrogen and the like in water bodies and environments, reduces nitrogen accumulation, protects the environments, and has a wide application prospect. The method has great application potential in livestock and poultry aquaculture and environmental protection.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

Nano imbibition displacement agent suitable for shale oil reservoir and preparation method of nano imbibition displacement agent

The invention provides a nano imbibition displacement agent suitable for shale oil reservoirs and a preparation method thereof, and the nano imbibition displacement agent comprises the following raw materials in parts by weight: 30-50 parts of sulfonated graphene oxide, 10-15 parts of silane coupling agent modified nano silicon dioxide, 10-20 parts of water-soluble cellulose ether, 10-20 parts of fatty alcohol-polyoxyethylene ether, 5-8 parts of lower alcohol and 50-80 parts of water. Graphene oxide reacts with concentrated nitric acid to prepare acidified graphene oxide, then the acidified graphene oxide reacts with thionyl chloride to prepare acylating-chlorinated graphene oxide, and the acylating-chlorinated graphene oxide reacts with a sodium aminobenzene sulfonate derivative to prepare the graphene oxide. Electrostatic repulsive force is generated among graphene oxide particles through surface sulfonation treatment, the dispersing capacity among the particles is improved, agglomeration and flocculation among the particles are avoided, and the recovery efficiency is improved; sulfonated graphene oxide cooperates with a large-molecular-weight water-soluble cellulose ether surfactant, so that the temperature resistance and the salt resistance of the nano imbibition displacement agent can be remarkably improved.
Owner:DAQING YONGZHU PETROLEUM TECH DEV CO LTD +1

Application of SlNAC61 gene and protein coded by SlNAC61 gene in regulation and control of salt tolerance of tomato

The invention is suitable for the technical field of molecular biology, and provides an application of an SlNAC61 gene and a protein coded by the SlNAC61 gene in regulating and controlling the salt tolerance of tomatoes. According to the invention, an SlNAC61 overexpression vector is successfully constructed and is introduced into a tomato plant, and an SlNAC61 overexpression strain is screened out. Under the condition of salt stress, the oxidative damage degree of leaves of an SlNAC61 overexpression plant is relatively light, which suggests that the SlNAC61 gene may influence the salt tolerance of the plant by regulating the accumulation of ROS in the plant. Besides, after long-time salt stress treatment, the wilting degree of the SlNAC61 overexpression strain is relatively light, and the reduction ratio of the plant height, stem diameter, fresh root weight and dry root weight is lower than that of a wild plant MT. The result shows that overexpression of the SlNAC61 gene can enhance the salt tolerance of the tomato plant, and an important theoretical basis and a practical basis are provided for subsequently cultivating a salt-tolerant tomato variety by using a gene engineering means.
Owner:JILIN UNIVERSITY

AvMYB1 protein and application thereof in improving salt tolerance of plants

The invention belongs to the technical field of genetic engineering, and particularly relates to an AvMYB1 protein and application thereof in improving salt tolerance of plants. The invention provides an AvMYB1 protein, wherein the amino acid sequence of the AvMYB1 protein is as shown in SEQ ID NO. 2. The AvMYB1 protein disclosed by the invention can be used for regulating the growth and development of plants and improving the stress resistance of the plants; according to the AvMYB1 protein, Na < + > is discharged by activating an SOS pathway, so that the Na < + > content in a plant body is reduced, and the salt tolerance of the plant is improved. The AvMYB1 gene is constructed into a plant expression vector, genetic transformation is carried out through an agrobacterium tumefaciens-mediated genetic transformation method, new germplasm with the salt-tolerant characteristic can be effectively obtained, and the method has important significance for developing salt-tolerant crop breeding.
Owner:TOBACCO RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES (QINGZHOU TOBACCO RESEARCH INSTITUTE OF CHINA NATIONAL TOBACCO COMPANY)

Biomass photo-thermal interface evaporation material based on nano TiO2-CNT photocatalytic thermal insulation layer, preparation method and application

The invention discloses a biomass photo-thermal interface evaporation material based on a nano TiO2-CNT photocatalytic thermal insulation layer, a preparation method of the biomass photo-thermal interface evaporation material and application of the biomass photo-thermal interface evaporation material in solar-driven interface evaporation, photo-thermal seawater desalination and sewage treatment. The preparation method comprises the following steps: S1, pulp pretreatment: softening pulp and removing saccharides and impurities; s2, preparing a carbonization layer; s3, a photocatalytic heat insulation coating is coated; S3.1, a photocatalytic heat insulation coating solution is prepared, wherein nanometer titania, carbon nanotubes, polyvinyl alcohol and lauryl sodium sulfate are taken and added into water, and a photocatalytic heat insulation layer solution is obtained; s3.2, a photocatalytic heat insulation coating is coated; and S4, pyrrole coating: uniformly coating the carbonized layer of the cactus pulp block with pyrrole, then uniformly coating the carbonized layer coated with pyrrole with the oxidant aqueous solution, and carrying out full polymerization reaction to obtain the biomass photo-thermal interface evaporation material Ppy / CTPS cactus.
Owner:GUILIN UNIVERSITY OF TECHNOLOGY

Method for identifying salt tolerance of asparagus in whole growth period in coastal saline-alkali land

The invention belongs to the technical field of plant breeding, and particularly relates to a method for identifying salt tolerance of asparagus in the whole growth period in coastal saline-alkali soil. According to the method, the salt tolerance of the variety is identified in the germination period, the seedling period and the field growth period of the asparagus, so that the salt tolerance of the whole growth period of the asparagus is comprehensively evaluated. In the germination period and the seedling period of the asparagus, a traditional sodium chloride stress method is changed into a saline-alkali soil leaching solution for salt stress treatment. According to the method, the salt tolerance of the asparagus varieties is evaluated by adopting a fuzzy comprehensive evaluation method in the field growth period of the asparagus, the contribution rates of a plurality of phenotype indexes under salt stress are comprehensively considered, the salt tolerance correction membership function value of each variety is calculated, and the limitation of evaluation by a single character index is avoided. According to the method, the salt tolerance of the variety can be reflected more comprehensively and more accurately from different growth periods of the asparagus.
Owner:河北省农林科学院经济作物研究所

Aramid nanofiber-based carbonaceous aerogel and preparation method thereof

The invention relates to aramid nanofiber-based carbonaceous aerogel and a preparation method thereof. The preparation method comprises the following steps: dispersing aramid fibers in an alkali solution, and washing with deionized water to obtain an aramid fiber dispersion liquid; performing vacuum filtration on the aramid fiber dispersion liquid, and performing freeze drying to obtain aramid fiber nanofiber aerogel; the aramid fiber nanofiber aerogel is subjected to carbonization treatment, and the aramid fiber nanofiber based carbonaceous aerogel is prepared. According to the preparation method disclosed by the invention, the highest evaporation rate of the obtained aramid nanofiber-based carbonaceous aerogel can reach 3.42 Kg / m < 2 > h by regulating and controlling the hydrolysis degree of the aramid fibers and combining vacuum suction filtration, and the aramid nanofiber-based carbonaceous aerogel has a good evaporation effect; in actual use, hydrophobic treatment is carried out on the upper surface of aerogel, and the defect that the photo-thermal evaporation effect of a product is reduced due to the fact that an open water layer is generated at a gas-liquid interface in the use process is effectively overcome.
Owner:SUZHOU UNIV

Application of coding sites of soybean PRR5a and PRR5b genes in regulation and control of salt tolerance

The invention relates to the technical field of biology, in particular to application of coding sites of soybean PRR5a and PRR5b genes in the aspect of regulating and controlling salt tolerance and application of a mutant material generated by the coding sites in the aspect of regulating and controlling the salt tolerance of soybeans. According to the invention, an effective target for editing soybean PRR5a and PRR5b genes is subjected to double-strand breakage under mediation of endonuclease Cas9, so that different allele mutants of PRR5a and PRR5b are obtained. By knocking out the PRR5a and PRR5b genes, a new strategy is provided for enhancing the salt tolerance of the soybeans, reliable means and materials are also provided for cultivating new varieties of the soybeans and improving the salt tolerance, and the method plays an important role in research and production of crops.
Owner:GUANGZHOU UNIVERSITY

Beta-glucosidase mutant D126T with improved activity and salt tolerance and application of beta-glucosidase mutant D126T

The invention discloses a beta-glucosidase mutant D126T with improved activity and salt tolerance, the amino acid sequence of the mutant is shown as SEQ ID NO.1, and compared with a recombinant wild enzyme with the amino acid sequence shown as SEQ ID NO.3, the activity of the mutant in metal salts with low concentrations of 10.0 mM and 50.0 mM and the enzyme activity in NaCl and KCl with the concentrations of 0.1 M-3. 0 M are improved. The activity of the mutant can be activated to different degrees by low-concentration metal salt, NaCl and KCl, the enzyme specific activity of the mutant D126T is improved to 93.5 U / mg under the optimal NaCl condition of 0.5 M, and compared with a control group without salt, the specific activity is improved by about 4.3 times; and the enzyme specific activity of the mutant is obviously improved compared with that of a wild enzyme. The D126T can have better applicability in the biotechnology field of a salt-containing environment.
Owner:YUNNAN NORMAL UNIV

Antibacterial bacillus subtilis subsp. Deserticola strain and application thereof

The invention relates to the technical field of microorganisms, in particular to an antibacterial bacillus subtilis subsp. Deserticola strain and application thereof.Bacillus subtilis subsp. Deserticola D7-HSJ9 is obtained from mangrove forest rhizosphere soil by the applicant, and it is detected that the strain has good salt resistance, alkali resistance and antibacterial characteristics, and the bacillus subtilis subsp. Deserticola D7-HSJ9 can be used as a bacillus subtilis subsp. Deserticola strain. According to the present invention, the bacterial strain has characteristics of good antibacterial effect, particularly good antibacterial effect on streptococcus iniae, and good antibacterial effect on a plurality of pathogenic bacteria such as photobacterium dama subspecies, aeromonas salmonicida, staphylococcus aureus and the like, such that the bacterial strain can effectively replace the antibiotic so as to improve the prevention and control of fish diseases, and a good germplasm resource is provided for later preparation of microecological preparation products.
Owner:BEIBU GULF UNIV

Application of rice ZOS2-02 gene in regulation and control of salt tolerance

The invention aims to disclose a rice salt tolerance related gene ZOS2-02 as well as a coding protein and application thereof. The gene ZOS2-02 is a DNA molecule as shown in 1) or 2): 1) a DNA molecule with a genomic sequence as shown in SEQ ID NO.1; 2) a DNA molecule with a CDS sequence as shown in SEQ ID NO.2; and 3) a DNA molecule which is hybridized with the DNA sequence limited by 1) or 2) under strict conditions and is used for coding the protein. According to the gene engineering application of the gene ZOS2-02 in regulation and control of the salt tolerance of the rice, specifically, the gene ZOS2-02 is knocked out, and the salt tolerance of the rice is improved.
Owner:NANJING AGRICULTURAL UNIVERSITY

Tracer agent for oil field and method for evaluating oil production contribution of each section of horizontal well by tracer agent

The invention provides a tracer agent for an oil field and a method for evaluating oil production contribution of each section of a horizontal well, and belongs to the technical field of oil field development. The tracer agent for the oil field, provided by the invention, is prepared from white oil, a nonionic surfactant, 3-allyloxy-2-hydroxy propanesulfonic acid sodium salt and alcohols; wherein the rare earth metal salt, the complexing agent and the sodium 3-allyloxy-2-hydroxy propanesulfonate form a complex; the complexing agent is a mixture of glycolic acid, triethylene tetramine and sodium dimercaptopropanesulfonate in a mass ratio of 10: (3-5): (1-2). The tracer agent for the oil field can stably exist under complex stratum conditions and can resist hypersalinity; in the process of evaluating the oil production contribution of each section of the horizontal well by using the tracer agent for the oil field, different types and different dosages of tracer agents are selected for different layer sections, so that the evaluation of the oil production contribution of each section of the horizontal well can be realized, and an effective basis is provided for subsequent refracturing.
Owner:DESHI (CHENGDU) PETROLEUM TECHNOLOGY CO LTD

Application of wheat TaSSRP1 protein and coding gene thereof in regulation and control of plant salt tolerance

The invention discloses application of a wheat TaSSRP1 protein and a coding gene thereof in regulation and control of plant salt tolerance. According to the invention, DNA molecules for coding TaSSRP1 protein are respectively introduced into wild type arabidopsis thaliana and wild type wheat, so that TaSSRP1 overexpressed arabidopsis thaliana and TaSSRP1 overexpressed wheat are respectively obtained. Experiments prove that under salt stress, compared with wild type arabidopsis thaliana and wild type wheat, the salt tolerance of TaSSRP1 overexpression arabidopsis thaliana and the salt tolerance of TaSSRP1 overexpression wheat are both improved, and it is indicated that the salt stress tolerance of plants is improved through TaSSRP1 overexpression. The invention discovers that the TaSSRP1 protein and the coding gene thereof have the function of regulating and controlling the salt tolerance of the plant for the first time, and the TaSSRP1 protein and the coding gene thereof have an important value for cultivating salt-tolerant plant varieties.
Owner:TIANJIN ACAD OF AGRI SCI

Graphene oxide composite oil displacement agent as well as preparation method and application thereof

The invention provides a graphene oxide composite oil-displacing agent and a preparation method and application thereof.The graphene oxide composite oil-displacing agent is prepared from poly (3-hydroxytyramine) and graphene oxide, the poly (3-hydroxytyramine) and the graphene oxide are combined through chemical bonds, the preparation method comprises the steps that hydrochloric acid 3-hydroxytyramine and the graphene oxide are subjected to a mixed reaction, and the graphene oxide composite oil-displacing agent is obtained. The obtained composite oil-displacing agent is applied to tertiary oil recovery. The composite oil displacement agent provided by the invention has excellent hydrophilicity, acid and alkali resistance and salt resistance, can form a stable water-in-oil emulsion with crude oil in a wide pH value range or under high salinity, and can improve the stability and viscosity of the emulsion; the emulsifying effect is strong, the emulsifying swept volume can be enlarged, and the application prospect in medium-low permeability oil reservoirs is wide; the preparation method provided by the invention is simple, convenient and high in practicability.
Owner:PETROCHINA CO LTD

Application of vaccinium myrtillus MYB transcription factor VcMYB4a gene in improvement of vaccinium myrtillus salt tolerance

The invention is applicable to the technical fields of molecular biology and biology, and provides application of a vaccinium myrtillus MYB transcription factor VcMYB4a gene in improvement of vaccinium myrtillus salt tolerance. The nucleotide sequence of the VcMYB4a gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoded protein of the VcMYB4a gene is as shown in SEQ ID NO: 2. The recombinant RNAi plant expression vector contains a specific RNAi sequence aiming at the C2 negative regulation region of the VcMYB4a gene. The constructed recombinant RNAi plant expression vector is transferred into the bilberry callus, the expression of the C2 negative regulation region of the VcMYB4a gene is inhibited, the phenotype of the transgenic callus is observed after salt stress treatment, and the result shows that the inhibition of the expression of the C2 negative regulation region of the VcMYB4a gene improves the salt tolerance of the bilberry callus.
Owner:JILIN UNIVERSITY

Analytical filler for separating glycosylated hemoglobin and preparation method thereof

The invention relates to the technical field of filler preparation, and discloses an analysis filler for separating glycosylated hemoglobin and a preparation method thereof.The analysis filler for separating glycosylated hemoglobin is obtained by synthesizing nonporous Polymer microspheres of 5 micrometers through a dispersion polymerization method, conducting hydrophilic treatment on the surfaces of the Polymer microspheres to obtain Polymer base balls and finally introducing ion exchange groups to the Polymer base balls. The hydrophilicity of the surface of the matrix is enhanced through hydrophilization treatment, non-specific adsorption is reduced, meanwhile, the swelling property of the filler in water is not affected, and the mechanical strength is not affected. The glycosylated hemoglobin analysis filler which is uniform in granularity, good in separation effect and long in service life can be obtained through the preparation method of the analysis filler.
Owner:SUZHOU HUADU BIOTECHNOLOGY CO LTD

Super-thick solvent-free epoxy coating for offshore steel pipe pile as well as preparation method and application of super-thick solvent-free epoxy coating

The invention discloses an ultra-thick solvent-free epoxy coating for an offshore steel pipe pile as well as a preparation method and application of the ultra-thick solvent-free epoxy coating. The ultra-thick solvent-free epoxy coating is prepared from a component A and a component B, the component A comprises the following components in percentage by mass: 20-25% of epoxy resin, 20-25% of silicon branched modified epoxy resin, 2-5% of a reactive diluent, 10-20% of calcium carbonate, 10-20% of natural barium sulfate, 2-5% of graphene, 10-15% of glass flakes, 5-10% of silica powder, 5-10% of zinc phosphate, 0.8-2% of a thixotropic agent and 0.2-0.3% of a flatting agent; and the component B comprises 15-30% of a curing agent, 15-25% of talcum powder, 15-25% of natural barium sulfate, 10-15% of titanium dioxide, 5-10% of zinc phosphate and 0.2-0.3% of a leveling agent. According to the present invention, the adhesion, the salt resistance, the wear resistance and the aging resistance of the epoxy coating can be improved, and the epoxy coating can be used for the anti-corrosion coating of the offshore steel pipe pile close to or in contact with seawater.
Owner:INTEGRITAS PAINT (HUANGSHAN) CO LTD

A kind of low pyruvate xanthan gum and preparation method thereof

The invention relates to the technical field of microbial fermentation, and specifically discloses a low-pyruvate xanthan gum and a preparation method thereof. The preparation method of the low-pyruvate xanthan gum comprises the following steps: S1, after activating xanthomonas by using an activation culture medium, transferring the activated strain into a seed culture medium for cultivation to obtain a seed liquid, inoculating the seed liquid into a fermentation culture medium, and obtaining a fermentation liquid after the cultivation is completed; S2, adding a treatment agent to the fermentation liquid obtained in step S1, stirring it, heating it to obtain a treatment liquid, extracting it with ethanol after cooling, drying it, and crushing it to obtain the treated liquid; the obtained low-pyruvate xanthan gum has excellent synergy, high temperature resistance, acid resistance, salt resistance and other properties, and has good product stability; the culture medium of the invention has simple components, a simple preparation method, saves time compared with mutating strains and improving strains, saves cost compared with optimizing culture medium components and adjusting culture control conditions, and is suitable for industrial production.
Owner:TONGLIAO HUANGHELONG BIOENG

Multi-layer composite fiber core and preparation method thereof

The invention provides a multi-layer composite fiber core and a preparation method thereof, and belongs to the technical field of disposable hygienic products. The preparation process comprises the following steps: preparing mesoporous silica nanoparticles; preparing composite super absorbent resin; preparing a bacteriostatic additive; preparing antibacterial and deodorant viscose fibers; preparing an antibacterial treatment solution; preparing an antibacterial treatment fiber web; and preparing the multi-layer composite fiber core body. According to the preparation method, carboxymethyl corn straw cellulose is mixed with acrylic acid, acrylamide and mesoporous silica nanoparticles, 2-acrylamide-2-methylpropanesulfonic acid is added, and reaction is performed under initiation of potassium persulfate, so that the water absorption rate of the prepared composite water-absorbent resin can be remarkably improved, multiple protection mechanisms can be formed, and the water absorption rate of the composite water-absorbent resin is improved. Interference of salt ions is prevented, so that the salt resistance of the composite water-absorbent resin can be effectively improved, and the water absorption rate of the composite water-absorbent resin to artificial blood / urine is further improved.
Owner:GUANGDONG XINROU PAPER CO LTD

Production method of salt-resistant drag reducer for oil well fracturing

The invention relates to the technical field of drag reducer preparation, and in particular to a method for producing a salt-resistant drag reducer for oil well fracturing. S1.1, weighing the following raw materials in parts by weight: 27-32 parts by weight of a modified polymer, 0.6-1.1 parts by weight of a cross-linking agent, 0.1-0.3 parts by weight of an antioxidant, 2-6 parts by weight of an acetate buffer, 0.6-0.8 parts by weight of a surfactant, 0.02-0.04 parts by weight of a benzalkonium chloride biocide, and 0.2-0.4 parts by weight of a bentonite suspending agent; wherein, the modified The polymer is prepared by copolymerization of acrylamide, crotonic acid and acrylic acid phosphonate in a mass ratio of 7:2 to 4:10 to 13; S1.2, adding the above raw materials and continuously stirring to obtain a salt-resistant drag reducer; crotonic acid, acrylic acid phosphonate and acrylamide are used to prepare a modified polymer, and carboxyl and phosphonic acid groups are introduced into the polyacrylamide, thereby enhancing the water solubility of the polymer and, at the same time, improving the coordination ability of the modified polymer with the zirconium ions in the cross-linking agent, thereby improving the salt resistance and drag reduction effect of the modified polymer.
Owner:山东德坤工贸有限公司

Chitinase-producing desert bacillus and its application

The present invention discloses a chitinase-producing desert bacillus and its application, and relates to the field of microbial technology. The present invention discovers a new microbial strain, which is identified as belonging to the genus Desert Bacillus and is named Desert Bacillus sp.MYHB_202305ChI. Experimental verification shows that the strain has the ability to tolerate high salt and secrete chitinase.
Owner:SI CHUAN HEBEN BIOTIC ENG