68results about How to "Improve salt tolerance" patented technology

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS2 Dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA1300 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS2 It is constructed from genes. SsNHX1 Genes and SsSOS2 The CDS coding sequence of the gene is shown in SEQ ID NO:1 and SEQ ID NO:2 of the sequence listing. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantage lies in the synergistic effect of the two genes, which effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

The invention belongs to the technical field of cosmetics, and particularly relates to a cosmetic delivery system based on a natural wall material, a preparation method and application. The cosmetic delivery system is of a core-shell structure, a core is a cosmetic active component, and a shell layer is a composite polymer gel network of sodium alginate, sophorolipid and cellulose. The natural polysaccharide composite wall material is green and safe, has good biocompatibility and strong compatibility with cosmetic formulas, and does not irritate the skin. The delivery system can effectively protect cosmetic active ingredients from being damaged by illumination and oxygen, and the storage stability of the active ingredients is remarkably improved; meanwhile, slow release and targeted transdermal absorption of active ingredients are realized, and the bioavailability is improved.

The invention discloses a preparation method of organic acid modified biochar as well as a product and application of the organic acid modified biochar, reference and a method are provided for resource utilization of a large number of waste grape branches, salicylic acid modified grape-based biochar obtained by modifying salicylic acid with the concentration of 50 mg / L and 500 mg / L has excellent specific surface area; therefore, excellent adsorption performance is obtained. According to the invention, the grape-based biochar obtained by modification with 500 mg / L of salicylic acid can improve the salt resistance level of eggplants under the stress of 100 mmol / L of NaCl. The invention provides a new method for efficient resource utilization of the rape straws. The rape straw biochar obtained by modifying the 100 mg / L ferulic acid can obviously improve the fresh weight of lettuce.

This invention relates to the field of genetic engineering technology, specifically disclosing the application of the SlCHP16 gene and its overexpression vector in tomato cultivation. The cDNA sequence of the SlCHP16 gene is shown in SEQ ID No. 1. Transforming tomato plants with the overexpression vector constructed using the SlCHP16 gene significantly promotes tomato root growth and significantly enhances the tomato's resistance to salt and alkali stress. The SlCHP16 gene overexpression vector provided by this invention is beneficial for promoting tomato root growth and enhancing the tomato's resistance to salt and alkali stress.

The purpose of this invention is to disclose a rice salt tolerance-related gene ZOS2-02, its encoded protein, and its applications. The gene ZOS2-02 is a DNA molecule as described in either 1) or 2) below: 1) a DNA molecule with a genomic sequence as shown in SEQ ID NO.1; 2) a DNA molecule with a CDS sequence as shown in SEQ ID NO.2; 3) a DNA molecule that hybridizes to the DNA sequence defined in 1) or 2) under stringent conditions and encodes the protein. The genetic engineering application of the gene ZOS2-02 provided by this invention in regulating rice salt tolerance specifically involves knocking out the aforementioned gene ZOS2-02 to improve rice salt tolerance.

The application relates to a gold nanoparticle array preparation method with strong stability and reusability, high cysteamine-stabilized gold nanoparticles are prepared first, the spacing between the gold nanoparticles is changed by utilizing the coulomb repulsion between the gold nanoparticles, then the gold nanoparticle solution is added dropwise to a glass surface modified by gamma-glycidoxypropyltrimethoxysilane to react, stable covalent bonds are formed, and gold nanoparticle arrays with uniform distribution on the glass surface can be obtained after the reaction. The preparation does not require high temperature, the experimental operation is simple and convenient, the gold nanoparticles are arranged in order and closely, have high detection sensitivity, have strong ultraviolet absorption capacity, good stability, reusability, salt resistance and low background interference, and can be reused. The gold nanoparticle array can realize detection analysis on high-salt-content samples in surface-assisted laser desorption ionization mass spectrometry and be used for rapid analysis of small molecule compounds in positive and negative ion dual modes.

The invention relates to the technical field of genetic engineering breeding, in particular to application of ZmC2DP1 protein in regulation and control of drought resistance and salt tolerance of plants, the amino acid sequence of the ZmC2DP1 protein is as shown in SEQ ID NO.1, and the nucleotide sequence of a gene for coding the ZmC2DP1 protein is as shown in SEQ ID NO.2. After the ZmC2DP1 gene is knocked out, the drought resistance and the salt resistance of a mutant plant under the drought treatment or salt treatment condition are improved compared with those of a control group plant, and it is verified that the ZmC2DP1 protein has a negative regulation function on the drought resistance and the salt resistance of the plant. Compared with a traditional breeding mode, the method for regulating and controlling the drought resistance and the salt resistance of the plants has the advantages that the breeding time is short, the purposiveness is high, the drought resistance breeding period is remarkably shortened, and the crop stress resistance breeding efficiency is improved; a gene editing homozygous strain with improved drought resistance and salt tolerance is obtained by adopting a gene knockout technology, and gene resources are provided for cultivating and improving new varieties of stress-resistant plants; and a theoretical basis is provided for clarification of a molecular mechanism of the ZmC2DP1 protein in drought resistance and salt resistance of plants.

This invention discloses a water-soluble anti-collapse agent, its preparation method, and a water-based slurry. The raw materials for the water-soluble anti-collapse agent include melamine, formaldehyde aqueous solution, polyol, epichlorohydrin, water, initiator, organophosphonate, and comonomer. The preparation method includes the following steps: mixing melamine and formaldehyde aqueous solution, adjusting the pH, and then reacting; introducing polyol into the reaction system to continue the reaction; then introducing organophosphonate solution into the reaction system, followed by epichlorohydrin to continue the reaction; finally introducing comonomer and initiator to continue the reaction, and then drying and optionally crushing to obtain the water-soluble anti-collapse agent. A water-based slurry containing the above-mentioned water-soluble anti-collapse agent is also provided. The anti-collapse agent provided by this invention has a filtration loss reduction rate of more than 70% against complex salts and a shale secondary rolling recovery rate of more than 80%. It has good salt resistance, temperature resistance, wall consolidation, and filtration loss reduction performance, and also has the advantages of being environmentally friendly, having low fluorescence, and good water solubility.

The application discloses a salt-resistant water-non-dispersible material for oil well cement and a preparation method thereof. The salt-resistant water-non-dispersible material for oil well cement comprises the following components in the form of weight parts: 100 parts of N,N-dimethyl acrylamide, 3-6 parts of acrylic acid, 10-20 parts of 2-acrylamide-2-methylpropanesulfonic acid, 5-15 parts of a cyclic monomer, 0.1-0.4 parts of a branching agent, 5-15 parts of a cationic monomer, 0.3-3 parts of a crosslinking agent and 400-500 parts of water. The salt-resistant water-non-dispersible material prepared by the application has good salt resistance, can significantly improve the anti-dispersion performance of the cement slurry when used in a salt water and sea water cement slurry system with a NaCl content of 18 wt% or less, and has excellent rheological performance.

This invention relates to the field of oilfield development technology, and discloses a polymer with viscosity-reducing function, its preparation method, and its application. The polymer has the structural units shown in Formulas 1 and 2. Oil displacement agents containing this polymer exhibit high static viscosity reduction, high oil recovery, salt resistance, and high-temperature resistance.

The application relates to the technical field of soil improvement, and discloses a grape planting soil improvement agent, which is prepared from the following components in parts by weight: 50-80 parts of attapulgite, 30-40 parts of calcium-based bentonite, 10-15 parts of molasses, 5-8 parts of N-dodecyl ethanolamine, 10-15 parts of polybutene diacid, 10-15 parts of polyacrylate, and 50-80 parts of water. The grape planting soil improvement agent has the advantages that the salt resistance of the polyacrylate is good, the water retention of the mineral is good, and the action time of the microbial agent is long.

This invention relates to the field of plant genetic engineering, specifically to the LbMYB75 gene of *Limonium bicolor* and its applications. This invention discloses that the transcription factor LbMYB75 is specifically highly expressed in the early stages of salt gland development and is significantly induced by salt. This invention confirms that the LbMYB75 gene positively regulates salt gland development and salt secretion in *Limonium bicolor*. Salt gland density is significantly increased in *Limonium bicolor* lines overexpressing the LbMYB75 gene, while salt gland density is significantly decreased in LbMYB75 mutant lines. The transcription factor LbMYB75 positively regulates the salt tolerance of *Limonium bicolor*; compared with the wild type, the overexpressing lines under high salt conditions show higher levels of malondialdehyde (MDA) and sodium. + and Na + / K + K decreased significantly. + Increased proline; This invention elucidates the regulatory role of the LbMYB75 transcription factor in salt gland development of *Limonium bicolor*, providing genetic resources and theoretical basis for improving the salt tolerance of non-halophytes through transgenic means, and contributing to the development and utilization of saline-alkali soils.

The invention belongs to the technical field of gene engineering, and particularly relates to a salt-tolerant gene ThGPX4 and application thereof. The invention provides a salt-tolerant gene ThGPX4. The salt-tolerant gene ThGPX4 is used for coding an amino acid sequence as shown in SEQ ID NO: 2. The invention finds that the ThGPX4 gene can significantly improve the salt tolerance of arabidopsis thaliana after overexpression in arabidopsis thaliana, the salt-tolerant gene ThGPX4 is constructed into a plant expression vector, genetic transformation is performed through an agrobacterium tumefaciens-mediated genetic transformation method, and a new germplasm with a salt-tolerant characteristic can be effectively obtained. The method is of great significance to cultivation of new varieties of excellent crops and wide application in production.

The application provides an autophagy-related gene ZmATG11a and application thereof in corn salt resistance, and the nucleotide sequence of the autophagy-related gene ZmATG11a is shown as SEQ ID NO. 1. The application discovers a new autophagy / salt resistance-related gene, and determines the correlation between autophagy and salt stress, thereby providing a new germplasm resource and theoretical basis for breeding salt-tolerant corn varieties.

The application discloses a kind of Lactobacillus plantarum for fermenting acid bamboo to increase aroma and quality and application thereof, specifically Lactobacillus plantarum SS0831 is isolated from natural fermentation acid bamboo, and has been preserved in China Center for Type Culture Collection on December 23, 2024, the preservation address is China, Wuhan, Wuhan University, and the preservation number is CCTCC NO:M20242877.The application uses the Lactobacillus plantarum to prepare fermentation acid bamboo, effectively increases the content of volatile aroma components such as esters, alcohols and aldehydes in fermentation acid bamboo, improves the flavor quality of acid bamboo, and provides people with a more pleasant sensory experience.

The invention discloses a medicago sativa MsCML69 gene for regulating and controlling salt tolerance and application of the medicago sativa MsCML69 gene. The nucleotide sequence of the MsCML69 gene is as shown in SEQ ID NO. 1; 1, and the corresponding amino acid sequence is as shown in SEQ ID NO. 2. The cDNA sequence of the calmodulin-like gene MsCML69 is cloned from medicago sativa, the obtained MsCML69 gene is connected with a plant expression vector to construct a recombinant expression vector, the constructed recombinant expression vector is used for transforming plant leaves, and finally transgenic plants are cultivated. Results show that overexpression of the gene can significantly improve the salt tolerance of the plant. The discovery of the MsCML69 gene provides a gene resource for cultivation of new stress-resistant germplasm of medicago sativa, the application range of the MsCML69 gene is widened, and the application of the MsCML69 gene has important production practice significance.

The invention relates to the technical field of microorganisms, and discloses a lactobacillus zeae strain and an application thereof, and the preservation number of the lactobacillus zeae strain is CGMCC (China General Microbiological Culture Collection Center) NO.26771. The lactobacillus zeae strain has the advantages that the lactobacillus zeae strain can be used for preparing a lactobacillus zeae strain; the lactobacillus zeae provided by the invention has the effects of inhibiting the activity of pathogenic bacteria, reducing inflammatory factors, inhibiting alveolar bone resorption and regulating digestive tract flora.

The application provides a high-temperature-resistant and quick-dissolving fracturing fluid thickening agent and a preparation method thereof, and belongs to the technical field of oil and gas well fracturing, and comprises the following steps: mixing and stirring acrylamide, 2-acrylamido-2-methylpropanesulfonic acid, acrylic acid, octadecyl methacrylate, pAMPS, an ammonium sulfate solution and 4-vinylpyridine propyl sulfobetaine, adding an additive, adjusting the pH value to 5.0-5.5, adding azobisdimethylaminoformamide hydrochloride to continue the reaction, washing, separating, vacuum drying to obtain a high-temperature-resistant and salt-resistant copolymer powder; adding the high-temperature-resistant and salt-resistant copolymer powder into deionized water and mechanically stirring, adding malt dextrin, sodium citrate and a hydrophilic additive into the deionized water in sequence under continuous stirring, continuing to stir, sieving, spray drying and granulating to obtain the high-temperature-resistant and quick-dissolving fracturing fluid thickening agent. The application can improve the high-temperature-resistant performance and the dissolution rate.

The application discloses a balsa wood / poly-p-phenylenediamine / polyvinyl alcohol solar energy evaporator and a preparation method thereof. NaClO2 and CH3COOH remaining in delignified balsa wood are used as oxidants to drive in-situ polymerization of p-phenylenediamine in a delignified balsa wood matrix, and the reuse of NaClO2 and CH3COOH is realized. The balsa wood / poly-p-phenylenediamine / polyvinyl alcohol evaporator prepared by the application has the characteristics of wide spectrum absorption, fast water transport, effective heat confinement and effective reduction of water evaporation enthalpy, and exhibits excellent solar energy driven interface water evaporation performance.

The application discloses a composite aerogel and a preparation method and application thereof, and belongs to the technical field of functional materials and environmental treatment. In the application, delignified cotton stalk cellulose is compounded with MXene to prepare a suspension, directional freezing self-assembly is carried out by using n-heptane as a heat transfer medium, and after freezing drying and polydimethylsiloxane dip coating, a PDMS / CMNF / MXene composite aerogel is obtained. The aerogel has an ultra-fine scale, high-density vertically arranged pore channels and a multi-level pore structure, and the pore channel diameter is 20-40 mu m. Under the driving of solar energy, the surface temperature of the aerogel can reach above 120 DEG C, the rapid in-situ viscosity reduction and efficient adsorption of high-viscosity crude oil are realized, the adsorption capacity is more than 56 times of the weight of the aerogel, and the performance retention rate is greater than 90% after 10 cycles. The application provides an efficient, green and recyclable solution for the treatment of marine high-viscosity crude oil leakage.

The present application relates to the technical field of oilfield chemistry, in particular to a salt-tolerant demulsifier system, application and a high-salt shale oil produced liquid treatment method.The salt-tolerant demulsifier system comprises a salt-tolerant block polyether main agent, a zwitterionic surfactant and an organic acid complexing agent; the salt-tolerant block polyether main agent is a star-shaped block copolymer with polyethylene oxide as a hydrophilic head, polypropylene oxide as a hydrophobic chain and polyglycerol or pentaerythritol as a starter; and the zwitterionic surfactant is a sulfobetaine surfactant.The salt-tolerant demulsifier system has excellent salt tolerance, high demulsification efficiency, fast speed and clean water quality; and the high-salt shale oil produced liquid treatment method has wide applicability and good effects on not only high-salt shale oil but also conventional high-salt crude oil produced liquid.

The invention discloses a Camel hoof valve gene ZbBCAT2 and application thereof to improvement of salt tolerance of plants. The CDS sequence of the Camel hoof valve ZbBCAT2 gene is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2. The invention further discloses a preparation method of the Camel hoof valve gene ZbBCAT2. The invention also discloses an application of the Camel hoof valve gene ZbBCAT2 in improving the salt tolerance of plants. The overexpression of the Camel hoof valve ZbBCAT2 gene plays a negative regulation role in the salt tolerance. The Camel hoof valve ZbBCAT2 gene provided by the invention can be used as a candidate gene for breeding the salt-resistant new variety of the plant, and has important significance for cultivating the salt-resistant new variety of the plant by adopting a genetic engineering method, accelerating the breeding process and reducing the breeding workload.

This invention relates to the field of plant genetic engineering technology and provides a bitter bean. SaATHB7 The cloning and application of genes involved transcriptome sequencing of *Bletilla striata* seedlings subjected to simulated abiotic stress. Differentially expressed genes were analyzed to screen for *Bletilla striata* genes associated with salt stress. The sequencing results revealed that these genes likely belong to the homologous domain leucine zipper family of transcription factors, and they were named... SaATHB7 Cloning and quantitative primers were designed based on the sequence obtained from sequencing. RT-PCR was used to detect the expression changes of this gene in different tissues of Sophora flavescens under abiotic stress conditions, in order to preliminarily study the corresponding role of this gene in abiotic stress of Sophora flavescens. At the same time, a plant overexpression vector of this gene was constructed and successfully transformed into wild-type Arabidopsis thaliana for preliminary functional verification. The results showed that overexpression of this gene in Arabidopsis thaliana can improve the salt tolerance and drought tolerance of Arabidopsis thaliana.

The application belongs to the technical field of thickening agent preparation, and particularly relates to a preparation method of a polymer thickening agent for fracturing. The preparation method comprises the following steps: preparing a pre-processed methyl methacrylate (triethoxysilyl) methyl ester solution; preparing a pre-processed 2-[[2-(methacryloyloxy)ethyl]dimethylammonium] acetate solution; preparing an aqueous phase; and preparing the polymer thickening agent for fracturing. The preparation method of the polymer thickening agent for fracturing comprises the following steps: taking acrylamide as a main monomer, taking maleic acid mono[2(1-oxo-2-propenyl)oxyethyl] ester, methyl methacrylate (triethoxysilyl) methyl ester and 2-[[2-(methacryloyloxy)ethyl]dimethylammonium] acetate as functional monomers, taking an oxidation-reduction initiator combined with an azo initiator as an initiator system, and realizing multi-monomer copolymerization through gradient temperature polymerization to prepare the polymer thickening agent for fracturing which has temperature resistance and salt resistance.

The invention discloses an emulsion type catalyst for hydrothermal cracking of thickened oil as well as a preparation method and application of the emulsion type catalyst, and belongs to the technical field of oil exploitation and catalysis. The catalyst is prepared by the following method: adding a molybdenum-based catalyst, an emulsifier and emulsion oil into water, and carrying out ultrasonic emulsification to obtain the thick oil hydrothermal cracking emulsion type catalyst. The emulsion type catalyst prepared by the invention is a nano molybdenum catalytic system with high stability, low oil phase proportion, wide pH application range and good salt tolerance, has good dispersity and transmission capacity in an oil-water reaction system, has relatively high catalytic cracking activity on underground thickened oil, and can be used for preparing the underground thickened oil. Therefore, the method has good applicability and good application value in the technical field of oil exploitation.

This invention belongs to the field of genetic engineering technology, specifically involving TaSCAMP17 The application of its recombinant overexpression vector in improving plant salt tolerance, the TaSCAMP17 The nucleotide sequence is shown in SEQ ID NO.1, and the amino acid sequence is shown in SEQ ID NO.2. This invention will... TaSCAMP17 Functional validation was performed in Arabidopsis thaliana after overexpression, and the results showed that overexpression... TaSCAMP17 The salt tolerance of wild-type Arabidopsis thaliana is significantly improved, therefore TaSCAMP17 It plays a positive regulatory role in wheat salt tolerance, providing a theoretical basis and gene resources for wheat molecular breeding, and is of great significance for obtaining salt-tolerant and high-yielding lines and improving wheat salt tolerance in the future.

The invention relates to the technical field of separation, and relates to chelate resin for removing calcium and magnesium ions in water as well as a preparation method and application of the chelate resin. The chelate resin for removing calcium and magnesium ions in water provided by the invention is composed of the matrix resin and the modified group, and the introduction of the modified group improves the adsorption capacity and stability of the matrix resin to the calcium and magnesium ions, and can respectively reduce the concentrations of calcium ions and magnesium ions in high-salt sewage to 0.5 ppm or below. And the capacity is still kept at 85% or above after 15 times of recycling, so that the sewage treatment cost is greatly reduced, and the method has a wide application prospect.

This invention belongs to the field of genetic engineering technology, specifically relating to a CtBBX4 protein, gene, and applications related to plant salt tolerance. This invention provides a CtBBX4 protein related to plant salt tolerance, the amino acid sequence of which is shown in SEQ ID NO.1. The CtBBX4 protein of this invention can improve the salt tolerance of plants. Examples show that overexpression of the CtBBX4 protein in plants... CtBBX4 Genes can increase plant germination rate and biomass, and improve plant salt tolerance by enhancing antioxidant capacity. CtBBX4 Genes are constructed into plant expression vectors and then genetically transformed using Agrobacterium-mediated transformation, which can effectively obtain new germplasm with salt tolerance characteristics, and is of great significance for the breeding of salt-tolerant crops.

The invention discloses an agriophyllum squarrosum AsWUS gene, a recombinant vector and application of the agriophyllum squarrosum AsWUS gene to improvement of plant stress resistance, and relates to the technical field of plant genetic engineering. The AsWUS gene is cloned from a high-stress-resistance wild plant agriophyllum squarrosum for the first time, and experiments prove that after the AsWUS gene is over-expressed in a model plant arabidopsis thaliana, the drought tolerance and salt tolerance of the plant can be remarkably improved. Precious gene resources and effective technical means are provided for molecular breeding of crops. The AsWUS gene, the recombinant vector and the recombinant bacteria provided by the invention have good application prospects in improving drought resistance and / or salt resistance of plants.

The application provides a binary ionic liquid fiber membrane, a preparation method and application in adsorption of palladium, and belongs to the technical field of adsorption materials, and comprises the following steps: mixing dichloromethane and acetic acid to obtain a first solution; dissolving cellulose acetate, a first ionic liquid and a second ionic liquid in the first solution to obtain a second solution; the first ionic liquid and the second ionic liquid are alkenyl imidazole salts with different length alkyl side chain molecular structures; adding N,N'-methylene bisacrylamide and benzoyl peroxide into the second solution, heating, stirring and warming to obtain a third solution; performing high-pressure electrospinning on the third solution to obtain a binary ionic liquid fiber membrane; or performing irradiation on the second solution after high-pressure electrospinning to obtain a binary ionic liquid fiber membrane. The binary ionic liquid fiber membrane prepared by the application can efficiently and selectively adsorb and recover palladium from a complex acidic solution, and has the advantages of faster adsorption speed, better selectivity and better salt resistance.