148 results about "Chloroauric acid" patented technology

The invention discloses a nano-enzyme detection method for aflatoxin B1, and relates to the technical field of analysis and detection.The method comprises the following specific steps that nano-enzyme preparation and carrier selection are conducted, specifically, nano-gold is prepared through a sodium citrate reduction method to serve as the nano-enzyme, a chloroauric acid solution is added into a round-bottom flask and heated to be boiled, and a carrier is obtained; according to the method, the PNIPAM serves as an immobilization carrier of the nano-enzyme, reversible immobilization of the nano-enzyme is achieved, cyclic utilization of the nano-enzyme is promoted, the detection cost is reduced, after detection is completed, phase change of the PNIPAM can be achieved by changing environmental conditions, and therefore effective separation of nano-gold and the carrier is achieved; after the recovered nanogold is washed and subjected to re-functionalization treatment, the catalytic activity of the nanogold is maintained, and the nanogold can be used for detecting aflatoxin B1 again, so that the utilization rate of the nano-enzyme is increased, and the waste of resources is reduced.

The application provides a preparation method of a SERS substrate of a zirconium-based MOF-808 / AuNPs. The method comprises three steps: in the first step, an AuNPs solution is prepared by using deionized water, chloroauric acid and sodium citrate to perform an oil bath reaction to obtain the AuNPs solution; in the second step, MOF-808 is prepared by mixing zirconium chlorohydrate, 1,3,5-benzene tricarboxylic acid, N,N-dimethylformamide and formic acid, and then performing a hydrothermal reaction on the mixed solution, centrifuging and vacuum drying to obtain activated MOF-808-Zr powder; in the third step, the activated MOF-808-Zr powder is dissolved in anhydrous ethanol, and then fully stirred and mixed with AuNPs solutions of different amounts to form a zirconium-based MOF-808 / AuNPs solution; and in the fourth step, the zirconium-based MOF-808 / AuNPs solution is poured into sample boxes containing silicon substrates, and the silicon substrates are immersed, and after drying, a SERS substrate of the zirconium-based MOF-808 / AuNPs is obtained. The method has the advantages of simple preparation process, low cost, good uniformity of the prepared substrate, good signal repeatability, high sensitivity, good structural stability and the like.

The application belongs to the technical field of fluorescent probe detection, and discloses a bimetallic nanocluster fluorescent probe compound and application thereof in preparation of a product for rapidly detecting acetylene. The fluorescent probe compound is a gold-copper bimetallic nanocluster solution modified by adamantane thiol, which is used as a fluorescent probe for acetylene detection. The solution has a clear and controllable preparation process. Through specific proportioning and reaction conditions of cuprous chloride, adamantane thiol, tetrachloroauric acid, sodium borohydride and other reagents, a probe solution with stable fluorescence characteristics can be prepared. The bimetallic nanocluster fluorescent probe compound is applied in preparation of a product for rapidly detecting acetylene. Through specific combination of fluorescent molecules and acetylene gas to trigger the secondary effect of fluorescence quenching, detection is realized, and other substances cannot trigger quenching. At a wavelength of 700 nm, the linear relationship (R 2 >0.99) between the fluorescence intensity and the acetylene concentration realizes accurate quantification of the acetylene concentration, and the minimum detection limit is 0.026 muL / L.

The invention provides a preparation method of high-sphericity gold powder, and relates to the technical field of nano material preparation. The preparation method comprises the following steps: sequentially adding a multi-ligand stabilizer solution and an acid solution into a chloroauric acid solution, and adjusting the pH value of a reaction system to 4.0-5.0 to obtain a solution A; then, quickly adding a dispersing agent solution into the solution A for first-stage mixing, and then, quickly adding a reducing agent solution for second-stage mixing; and then, a morphology optimizing agent solution is dropwise added into a reaction system, precipitates are collected after full reaction, and the high-sphericity-degree gold powder is obtained. According to the method, a multi-ligand stabilizer is added into a chloroauric acid solution, and accurate control over the morphology of the gold powder is achieved through competitive adsorption between ligands. On the basis, an acid solution is added into a chloroauric acid solution, morphology control over the gold powder is further consolidated, the pH value of the solution adjusted through the acid solution is acidic, the reduction rate is further decreased, and the gold powder is better in dispersity and more uniform in size.

The application provides a preparation method and application of a flexible electrochemical sensor and belongs to the technical field of electrochemical detection. The preparation method is as follows: first, polyurethane sponge is soaked in a conductive carbon paste dispersion solution to obtain a conductive support; second, the conductive support is soaked in an aqueous chloroauric acid solution for electrochemical deposition to obtain a gold nano-structure layer modified conductive support; third, the gold nano-structure layer modified conductive support is placed in a groove of a PDMS base, and the gold nano-structure layer modified conductive support is separated into a working area and a clamping area by pouring PDMS to obtain a working electrode; and finally, a flexible electrochemical sensor is assembled. The flexible electrochemical sensor is used for hydrogen peroxide detection and has good biocompatibility, excellent tensile properties and stable electrochemical sensing performance.

The invention discloses a colorimetric detection method for cMyBP-C protein based on nano-enzyme activity, preparation of a bismuth selenide / nano-gold composite material and application of the bismuth selenide / nano-gold composite material in protein detection. The method comprises the following steps: performing ultrasonic stripping on a bismuth selenide block to obtain a few-layer bismuth selenide dispersion liquid; and then sequentially adding a sodium borohydride aqueous solution and a chloroauric acid aqueous solution under a stirring condition, and carrying out in-situ reduction to form gold nanoparticles loaded on the surface of bismuth selenide. The composite material has the capability of catalyzing reduction of p-nitrophenol into p-aminophenol, and the color of the solution is changed from yellow to colorless. After the cMyBP-C antibody is modified on the surface of the composite material, the catalytic activity is inhibited; after a to-be-detected sample is added, the antibody and the protein are specifically combined to cause dissociation, and the catalytic activity is recovered. The cMyBP-C protein is quantitatively detected by observing the color change of the solution or monitoring the reduction of the ultraviolet absorbance at 400 nm.

This invention discloses gold nanoparticle-modified metal oxide framework materials, their preparation methods, and applications, relating to the fields of material preparation and detection technology. The preparation method of the gold nanoparticle-modified metal oxide framework material includes: in-situ reduction of chloroauric acid on the surface of ZIF-67 via a reduction method to obtain an Au / ZIF-67 composite material; and calcination of the Au / ZIF-67 composite material using a programmed temperature rise process to obtain the final product. This invention also provides applications of this material in serum metabolite detection and in screening metabolic biomarkers for esophageal cancer diagnosis. The composite material prepared by this invention possesses a unique hollow porous structure and uniformly distributed gold nanoparticles, providing a large specific surface area for enriching metabolites in serum. It also enhances the ultraviolet absorption and photothermal conversion efficiency of the nanocomposite material, while improving the material's conductivity, thereby significantly improving laser desorption / ionization efficiency and achieving high-sensitivity detection.

The invention relates to a pyridine compound modified CuAu bimetallic nano hollow structure catalyst, and preparation and application thereof, and belongs to the technical field of electro-catalysis. The preparation method comprises the following steps: dropwise adding a chloroauric acid solution into hollow Cu2O nano-particles to obtain a core-shell structure with gold nano-particles loaded on the surfaces of the hollow Cu2O nano-particles, then carrying out solvothermal reaction to obtain hollow CuAu nano-particles, and then adding a pyridine organic modifier to obtain the hollow CuAu nano-particles. The catalyst prepared by the invention has a Cu < + > / Cu0 at Au functionalized catalytic interface, realizes differential adsorption of * CO intermediates and subsequent hydrogenation reaction process, and promotes asymmetric * CO-* CHO coupling, so that the activity and selectivity of ethanol products are remarkably improved, and the Faraday efficiency can reach 76%.

This invention belongs to the field of biosensing technology, specifically relating to a modified carbon fiber electrode for detecting dopamine, its preparation method, and its application. The modified carbon fiber electrode comprises a carbon fiber electrode onto which gold-platinum bimetallic nanoparticles and polydopamine are sequentially modified. The preparation method specifically includes: firstly, modifying the surface of activated carbon fiber filaments with chloroauric acid and sodium citrate to obtain Au@CF; then, modifying the Au@CF surface with ascorbic acid and chloroplatinic acid to obtain platinum nanoparticles, obtaining Au / Pt@CF; finally, immersing the Au / Pt@CF in a dopamine solution to coat the Au / Pt@CF surface with polydopamine, obtaining PDA@Au / Pt@CF. This electrode has advantages such as high sensitivity, low detection limit, strong anti-interference ability, good biocompatibility, and high mechanical stability, making it suitable for dynamic monitoring of dopamine in the living brain.

This invention relates to a near-infrared low-potential electrochemiluminescence dual-ligand stabilized gold nanoclusters, their preparation method, and applications. The method uses chloroauric acid as the gold source and thiomalic acid and sodium citrate as reducing and stabilizing agents, respectively, in a two-step synthesis. First, Au is reduced online using thiomalic acid. 3+ Water-soluble gold nanoclusters were prepared using a novel method. These nanoclusters were then incubated with sodium citrate, and based on the ligand exchange principle, gold nanoclusters co-coated with thiomalic acid and sodium citrate were synthesized. The raw materials required for this invention are readily available and all possess good water solubility. The synthesis apparatus is simple, the conditions are mild, and the operation is safe. The resulting dual-ligand-coated gold nanoclusters exhibit good stability and produce low-potential near-infrared electrochemiluminescence in the long-wavelength region in an aqueous system. This overcomes the limitation of most current electrochemiluminescent materials requiring hydrazine hydrate or carbazide as a co-reactant. Using a non-toxic tert-butylamine-borane co-reactant, low-potential electrochemiluminescence radiation of 0.57V in the near-infrared region was generated.

The invention discloses a preparation method of silicon dioxide-gold core-shell nanoparticles, and belongs to the field of nano material preparation. The method comprises the following steps: preparing aminated silicon dioxide nanoparticles; preparing small gold nanocrystal seeds and adsorbing the small gold nanocrystal seeds on the surface of silicon dioxide; ascorbic acid or a salt thereof is used as a weak reducing agent, a chloroauric acid solution is slowly added, uniform growth of a gold shell is induced on the surface of a gold seed, and finally a complete core-shell structure is formed. According to the method, surface modification and adsorption are optimized, a weak reduction growth system is adopted, and growth kinetics is accurately controlled, so that the problems of uneven gold shell coating, complex process and poor reproducibility in a traditional method are effectively solved. The nanoparticles prepared by the method have the advantages of complete and smooth gold shell, accurate and controllable thickness (5-50nm), adjustable plasma resonance absorption, good monodispersity and the like, and have important application value in the fields of biomedicine and photoelectricity.

The invention belongs to the field of preparation of organic-inorganic hybrid materials, and relates to a preparation method and application of cucurbit [n] uril-gold nanoparticles. Through a simple one-pot synthesis method, chloroauric acid, silver nitrate, a reducing agent and cucurbit [n] uril are added for co-reduction to prepare hollow gold nanoflowers; the gold nanospheres with different sizes are prepared by regulating and controlling the input amount of cucurbit [n] uril. The gold nanoparticle hybrid material shows excellent hydrogen evolution side reaction inhibition capability in the application of electro-catalysis CO2 reduction, and the CO selectivity is obviously improved.

The application discloses a Raman enhancer and a preparation method and application thereof, and adopts an improved trisodium citrate reduction chloroauric acid method to prepare dopamine carbon dot modified gold nano Raman enhancer by taking dopamine carbon dot as a reducing agent and a stabilizer. When the Raman enhancer is used for detecting imazalil residues in tobacco, the dopamine carbon dot modified gold nano Raman enhancer is prepared, a linear relationship between the imazalil concentration and the peak area of a quantitative characteristic peak is established, and the surface enhanced Raman spectrum of the imazalil in the tobacco sample is detected. The dopamine carbon dot is synthesized for the first time, the dopamine carbon dot is introduced into the preparation of the gold nano Raman enhancer for the first time and is used as a reducing agent and a stabilizer, trisodium citrate is used as a reducing agent to reduce chloroauric acid, and a new method for synthesizing the gold nano Raman enhancer is provided. The gold nano has a remarkable Raman enhancement effect and the stability is remarkably enhanced. The gold nano can be stored in a refrigerator for more than half a year without agglomeration, can be stored at room temperature for a short period of time, and the storage does not affect the enhancement effect.

The invention provides a microbial spore rapid detection reagent, a preparation method thereof and a microbial spore rapid detection method, and relates to the field of microbial detection. The preparation method of the rapid microbial spore detection reagent comprises the following steps: mixing a chloroauric acid aqueous solution and amino acid, adding a sodium citrate buffer solution to adjust the pH value of the system to be weakly acidic, and then dropwise adding a sodium borohydride aqueous solution to carry out a reduction reaction to obtain a colloid; the colloid is subjected to purification treatment, and then an AuNPs solution is obtained; and adjusting the absorbance A520 of the AuNPs solution by using a sodium citrate aqueous solution, then adding a rare earth metal salt aqueous solution with the same volume as the AuNPs solution, and standing and incubating to obtain the rapid detection reagent for the microbial spores. According to the preparation method of the microbial spore rapid detection reagent provided by the invention, the obtained detection reagent can realize extremely high sensitivity and resolution, and the actual detection limit of a standard DPA solution can reach 1pM.

The application discloses a kind of synergistic antitumor porphyrin-based covalent organic framework heterojunction materials and preparation method thereof, belong to biological medicine technical field. 5,10,15,20-tetra (4-aminobenzene) -21H,23H-porphyrin and 2,5-divinyl benzene formaldehyde are added to organic solvent to carry out solvothermal reaction, and porphyrin-based covalent organic framework (pCOF) is obtained;pCOF is mixed with 1,2-ethanedithiol and heated to obtain thiolated modified pCOF;Thiolated modified pCOF is stirred with glutathione aqueous solution ultrasonically, then tetra-chloroauric acid is added to carry out reaction, and synergistic antitumor porphyrin-based covalent organic framework confined gold nanocluster heterojunction material, recorded as Au@pCOF, is obtained.The Au@pCOF prepared in the application can be used as a kind of biocompatible antitumor therapeutic agent, under the irradiation of 808 nm near-infrared light, active oxygen is efficiently generated and heat is released, photothermal and photodynamic synergistic enhancement is realized, tumor cells are significantly killed, and is used for space-time integration synergistic antitumor therapy.

The application relates to a kidney-excretable nano-gold aggregate, a preparation method and application as a CT contrast agent, and comprises the following steps: mixing chloroauric acid solution, lye and a mercapto hydrophilic compound to obtain mixed solution A; under the condition of ice water bath and stirring, a reducing agent is added into the mixed solution A to obtain mixed solution B containing nano-gold particles; the mixed solution B is mixed with a wrapping material-buffer solution to obtain mixed solution C containing nano-gold particles@wrapping material; a positively charged compound solution is added into the mixed solution C to obtain mixed solution D; the nano-gold is made into a stable aggregate by the positively charged compound; and the mixed solution D is centrifuged to obtain the kidney-excretable nano-gold aggregate. The synthesized super-small nano-gold is agglomerated into a nano-gold aggregate with stable structure, and the contrast agent can be obtained by simple centrifugal concentration, the contrast agent is stable in property, has good biocompatibility, and can be discharged out of the body along with urine within a period of time after entering the body.

The invention relates to a fluorescent-Raman dual-mode biosensor as well as a preparation method and application thereof, and belongs to the technical field of biological detection. The preparation method comprises the following steps: S1, adding a chloroauric acid solution into an alkaline solution, stirring for the first time, continuously adding UiO-66-N3, a polyvinylpyrrolidone solution and a reducing agent, stirring for the second time, centrifuging and resuspending to obtain a fluorescence-Raman dual-mode substrate solution; and S2, adding the mucoprotein 1 aptamer solution into the fluorescence-Raman dual-mode substrate solution to perform primary incubation, performing centrifugation, washing and resuspension, adding the mitochondrial targeting peptide solution to perform secondary incubation, and performing centrifugation, washing and resuspension to obtain the fluorescence-Raman dual-mode biosensor. The dual-mode biosensor realizes high-fidelity, high-spatial-resolution and high-reliability dual-mode imaging analysis of hydrogen sulfide.

The application discloses a kind of CL-AuNPs@CD-MOF / Zein-PLA antibacterial films and preparation method and application thereof.The application is prepared gold nanoparticle (AuNPs) by green synthesis strategy, with CD-MOF as stabilizer, p-coumaric acid (p-CA) as natural reducing agent, by reducing tetrachloroauric acid (HAuCl4), CD-MOF is obtained by covalent crosslinking modification CL-AuNPs@CD-MOF with diphenyl carbonate (DPC), finally it is introduced into corn alcohol-soluble protein (Zein)-polylactic acid (PLA) composite film, utilize Zein excellent oxygen barrier property and PLA excellent mechanical property mutual coordination, construct the respiration microenvironment with suitable water vapor and oxygen permeability, so as to effectively inhibit the proliferation of microorganism on the surface of agaricus bisporus, and maintain its sensory quality.The composite film has significantly enhanced water stability, realizes the sustained release of gold nanoparticles, can effectively inhibit the microbial infection of agaricus bisporus after harvest.

A method for preparing bimodal nanocapsules includes: preparing a solution of mesoporous silica and deionized water, adding chloroauric acid solution and stirring, then adding sodium borohydride solution under ice bath stirring conditions to obtain gold nanoparticle-modified mesoporous silica; preparing a solvent of ultrapure water and gold nanoparticle-modified mesoporous silica, then sequentially adding a fluorescence quencher, a fluorescent molecule, and 4-mercaptophenylboronic acid to react separately to obtain bimolecularly modified nanoprobes; and mixing an adenosine-5′-triphosphate nucleic acid aptamer solution with the nanoprobes using ultrapure water as a solvent to obtain nucleic acid aptamer-encapsulated bimodal nanocapsules. This invention provides a simple, mild, environmentally friendly, and low-cost preparation method. The bimodal nanocapsules prepared by this invention can simultaneously monitor the levels of hydrogen peroxide and adenosine-5′-triphosphate (ATP) in cells using fluorescence and surface-enhanced Raman scattering (SERS) techniques, which can be used for early disease monitoring.

This invention discloses Au nanoparticles loaded with bimetallic compounds for photocatalysis and their preparation method, belonging to the field of photocatalytic materials technology. Preparation steps: S1: Tetrachloroauric acid, quaternary ammonium salt, and citric acid are added to a sodium borohydride solution and stirred at 25-120℃ for 1-3 hours to obtain solution A; solution B is prepared containing quaternary ammonium salt, tetrachloroauric acid, ascorbic acid, silver nitrate, and hydrochloric acid; solutions A and B are mixed and stirred at 25-50℃ for 1-15 hours to obtain Au nanoparticles; S2: Solution C is prepared containing quaternary ammonium salt, a first metal source, and ascorbic acid; solution D is prepared containing quaternary ammonium salt, a second metal source, NaOH, and ascorbic acid; Au nanoparticles are mixed with solution C and reacted at 40-80℃ for 1-5 hours; then the supernatant is removed by centrifugation, and solution D is added again, reacting for 1-5 hours to obtain Au nanoparticles loaded with bimetallic compounds. This invention enables controllable nanoparticle structure and allows for metal loading at different sites by adjusting synthesis parameters.

The invention discloses an alkali metal modified ZnO-Au composite aerogel as well as a method and application thereof, and belongs to the technical field of aerogel material preparation. The method comprises the following steps: 1) dispersing zinc acetate dihydrate, alkali carbonate and chloroauric acid in a mixed solvent of water and ethylene glycol, and stirring and mixing to form a suspension; 2) adding a small amount of nitric acid into the mixed solution to obtain a clear solution; 3) adding citric acid into the clarified solution, and standing at a certain temperature to form gel; the preparation method is simple and convenient to operate, the raw materials are easy to obtain, the preparation period is short, and the prepared aerogel can be widely applied to various catalytic reactions.

The invention discloses a gold (I) complex containing an alkyne ligand, a preparation method of the gold (I) complex and application of the gold (I) complex in preparation of antitumor drugs, the gold (I) complex has a structure as shown in a general formula I, a phosphorus ligand is selected from diphenyl-2-pyridine phosphine or tricyclohexylphosphine, and Rx is an organic group selected from a specific alkyne ligand; according to the preparation method, chloroauric acid is taken as an initial raw material, sequentially reacts with dimethyl sulfide and an organic phosphine ligand, and finally is coupled with an alkyne ligand under an alkaline condition, so that the steps are simple and convenient, the conditions are mild, and the yield is high; in-vitro activity experiments show that the complex shows remarkable proliferation inhibition activity on ovarian cancer, malignant melanoma, non-small cell lung cancer and cisplatin-resistant cell strains thereof, the effect of the complex is superior to that of cisplatin and auronafine, and a candidate compound is provided for developing a novel targeted anti-tumor drug for overcoming the drug resistance of platinum drugs.

The application belongs to the field of nanomaterials and biotechnology, and particularly relates to application of non-ROS-dependent photoenzyme in photocatalysis and Nrf2 activation. The preparation method of the non-ROS-dependent photoenzyme is as follows: chloroauric acid is added into a mixed solution of 6-azido-2-thiothymine and sodium hydroxide, and the reaction is stirred at 25-30 DEG C to prepare nanometer photoenzyme; the molar ratio of 6-azido-2-thiothymine to chloroauric acid is (2.1-2.6):1. The non-ROS-dependent nanometer photoenzyme provided by the application has the advantages of simple and efficient synthesis method, ultrahigh photocatalytic activity, non-ROS-dependent catalytic mechanism, excellent biocompatibility, and the ability to realize intracellular light-controlled activation of Nrf2, and solves the problems of ROS-dependent oxidative damage, uncontrollable Nrf2 activation, and over-activation of signal pathways.

This invention relates to the field of nanoscale full-spectrum photocatalytic materials, and particularly to a method for preparing a molybdenum selenide / oxygen-deficient bismuth oxybromine@gold three-phase Z-type heterojunction photocatalyst. This method solves the problems of low photogenerated electron-hole separation efficiency, narrow full-spectrum response range, and poor stability in existing bismuth-based photocatalysts. The preparation method involves first preparing oxygen-deficient BiOBr nanospheres via a solvothermal method, then directly reacting tetrachloroauric acid with oleylamine in toluene to prepare small-particle-size monodisperse Au nanoparticles, which are then combined with oxygen-deficient BiOBr to prepare a BiOBr@Au nanosphere core-shell structure. Finally, a MoSe2 / oxygen-deficient BiOBr@Au three-phase composite material is prepared via a hydrothermal reaction. The catalyst of this invention exhibits absorption capacity under the full spectrum of light, and simultaneously achieves a synergistic enhancement of carrier separation efficiency and strong redox capability, demonstrating excellent photocatalytic activity and the ability to efficiently degrade pollutants and produce hydrogen.

The invention discloses a nanogold polymer composite material for surface enhanced Raman scattering (SERS) and a preparation method of the nanogold polymer composite material. The material is prepared from a monomer, a cross-linking agent, a photoinitiator, tetrachloroauric acid and water. The preparation method comprises the following steps: preparing a gold source precursor solution, preparing a photocuring premixed solution, mixing to form liquid photosensitive resin, carrying out ultraviolet light curing molding to form a composite film, and carrying out heat treatment to obtain a final product. According to the method, a reducing agent and subsequent purification are not needed, polymer generation and nanogold reduction are synchronously achieved through one-step photopolymerization, and the method is simple in process, mild in condition and environmentally friendly. The obtained nanogold is mainly triangular, polygonal and polyhedral, has rich tip and edge structures, is uniformly dispersed in a polymer without agglomeration, can effectively form SERS hot spots, and is suitable for an SERS substrate.

The invention belongs to the field of catalytic materials, and particularly discloses a high-stability nanogold-loaded molecular sieve catalyst for catalyzing methane selective oxidation. The catalyst is prepared by a two-step synthesis method. The method comprises the following steps: recrystallizing an MFI structure molecular sieve carrier, centrifuging, washing, drying and calcining to obtain the modified molecular sieve. Then mixing a chloroauric acid solution with the modified molecular sieve carrier, adjusting a certain pH value, stirring and aging for a certain time at a proper water bath temperature, and then filtering, washing, drying and calcining to obtain the catalyst. The catalyst can realize direct oxidation of methane into methanol and acetic acid by oxygen, has high stability, and can still maintain catalytic activity under long-time reaction and multiple catalytic reaction cycle test conditions. The preparation method of the catalyst is simple in process, and a thought is provided for design of a high-efficiency catalyst in methane oxidation reaction.

This invention provides a method for modifying Ti3C2T with nano-gold. x This invention relates to quantum dot composite SERS substrate materials, their preparation methods, and applications, belonging to the field of detection and testing technology. The invention involves etching Ti3AlC2 material to obtain Ti3C2T... x The material was diluted with ammonia and then crushed to obtain Ti3C2T. x Materials: Quantum dot suspension; Chloroauric acid solution was mixed with ascorbic acid, surfactant, and water to carry out a reduction reaction, yielding nano-gold sol; Ti3C2T x The quantum dot suspension was mixed with gold nanoparticle sol for composite formation, and the resulting gold nanoparticles were used to modify Ti3C2T. x Quantum dot composites were attached to a solid support to obtain Ti3C2T based on gold nanoparticles. x Quantum dot composite SERS substrate material. The composite SERS substrate material prepared by this invention achieves efficient detection of hydrophobic organic compounds and can be widely used in the field of biodetection technology, demonstrating strong practicality.

The invention discloses an immunosensor for detecting porcine epidemic diarrhea virus as well as a preparation method and a detection method of the immunosensor. The preparation method of the immunosensor for detecting the porcine epidemic diarrhea virus comprises the following steps: S1, sequentially adding H2O2 and concentrated nitric acid into an aqueous solution of tungstate for reaction to obtain a modification solution; s2, performing electrochemical deposition on the glassy carbon electrode by taking the modification liquid as electrolyte; s3, taking an aniline solution composed of concentrated sulfuric acid, aniline and water as an electrolyte, and performing electrochemical polymerization on the glassy carbon / tungsten oxide electrode; s4, placing the glassy carbon / tungsten oxide / polyaniline electrode in a chloroauric acid solution for electro-deposition; s5, dispensing a porcine epidemic diarrhea virus antibody on the surface of the glassy carbon / tungsten oxide / polyaniline / gold electrode, and incubating; and S6, dropwise adding bovine serum albumin on the surface of the glassy carbon / tungsten oxide / polyaniline / gold-antibody electrode, and sealing. The sensor has the advantages of high detection speed, high stability, strong anti-interference performance and the like.

The application provides a sub-micron spherical gold powder and a preparation method thereof, and belongs to the field of noble metal powder. The application provides a preparation method of the sub-micron spherical gold powder, which comprises the following steps: adding a first alkylamine, a first reducing agent and a chloroauric acid solution into a first aqueous medium to obtain a gold crystal nucleus solution; adding a second alkylamine into the gold crystal nucleus solution, and then adding a second reducing agent and a chloroauric acid solution drop by drop to make gold grow on the surface of the gold crystal nucleus to obtain a precipitate; and performing post-treatment on the precipitate to obtain the sub-micron spherical gold powder; wherein the number of carbon atoms in the alkyl chain of the first alkylamine is 14-18, and the number of carbon atoms in the alkyl chain of the second alkylamine is 10-18. Through the cooperation of the first alkylamine and the second alkylamine, the particle size of the gold powder can be controlled in the range of 0.1 mu m-1.5 mu m, the dispersion is good, the morphology is regular, and it is beneficial to form a dense gold film during sintering.

The invention discloses a lactic acid molecular imprinting material, a preparation method thereof and a molecular imprinting biosensor, and the preparation method comprises the following steps: taking melamine as a raw material, and calcining in a muffle furnace to obtain multi-layer carbon nitride; the preparation method comprises the following steps: adding trititanium aluminum dicarbon powder into an etching agent solution composed of lithium fluoride and a hydrochloric acid solution, then carrying out drying and intercalation treatment on a mixed solution, and carrying out centrifugal purification to obtain single-layer MX alkene; respectively dispersing the prepared carbon nitride and MX alkene in ultrapure water, mixing, sequentially adding a chloroauric acid solution and a sodium citrate solution, and reacting to obtain a carbon nitride / MX / gold composite material; a deposition solution is prepared by taking a phosphate buffer solution as a solvent, the deposition solution and a screen-printed carbon flexible electrode dripped with the carbon nitride / MX / gold composite material are subjected to electro-deposition, and then the lactic acid molecular imprinting material can be obtained by washing with an acetic acid solution. According to the invention, lactic acid molecules are specifically recognized, in-situ and noninvasive detection of lactic acid in sweat is carried out, and accurate, rapid and sensitive PEC flexible detection is realized.