122 results about "Immunoassay method" patented technology

The present invention is directed to assay methods and compounds and kits used in such assays.

The invention discloses a YOLO-Drop-based 3D printing droplet microfluidic immunoassay method, which organically integrates a high-precision microfluidic chip preparation technology, a droplet-level immunoreaction system and a fluorescence imaging acquisition and deep learning detection algorithm. And full-chain closed-loop processing from droplet generation, fluorescence signal acquisition, image recognition, result statistics to concentration quantitative analysis is realized. Through deep combination of chip physical parameters and model input features, the method not only significantly improves the accuracy and stability of droplet identification and concentration quantification, but also enhances the robustness of the algorithm under complex sample conditions through multi-scale feature fusion and a signal dual screening mechanism. Meanwhile, the optimized YOLO-Drop model supports embedded efficient reasoning, so that the whole analysis process has the characteristics of real-time performance and high throughput, and the method is suitable for rapid quantitative detection of various biomarkers.

The invention belongs to the technical field of immunodetection, and discloses an avian single-chain antibody for detecting a liver cancer marker GPC3, a kit and a detection method. The poultry-derived single-chain antibody has the characteristics of small volume, high specificity, high stability and good sensitivity, and can be used in an immunoassay method for detecting a liver cancer marker GPC3; according to the method, a chicken-derived single-chain antibody is coated on a solid-phase carrier, a rabbit-derived polyclonal antibody is taken as a detection carrier, the antibodies are combined with a GPC3 antigen to form a sandwich structure, and the double-antibody sandwich immunoassay method of the GPC3 is established by using the method, and has the advantages of high specificity, good sensitivity, high sensitivity and high sensitivity. The invention provides a rapid, accurate and reliable detection method for detecting GPC3 in serum.

The invention belongs to the technical field of immunodetection, and discloses a preparation and characterization method of a rabbit-derived single-chain antibody for detecting a liver cancer marker GPC3. The rabbit-derived single-chain antibody has the characteristics of small volume, high specificity, high stability and good sensitivity, and can be used in an immunoassay method for detecting a liver cancer marker GPC3; according to the method, a specific rabbit single-chain antibody is obtained through screening, expression and purification by using a phage display technology, a GPC3 antigen is coated on a solid-phase carrier, the rabbit single-chain antibody is used as a detection carrier, the binding force of the single-chain antibody and the antigen is identified, and the method is high in specificity, good in sensitivity and high in specificity. A novel single-chain antibody with high specificity and high sensitivity is provided for detection of GPC3 in serum.

Described herein are immunoassay methods for detecting and / or monitoring a cancer in a patient. In the method a biofluid sample from a patient is contacted with a monoclonal antibody that specifically binds to a C-terminal epitope of type XXVIII collagen, and the amount of binding between the monoclonal antibody and peptides in the sample is detected and determined.

The invention comprises a micro-fluidic chip and an immunodetection method. The micro-fluidic chip comprises a reaction layer substrate and a micro-channel sample adding layer positioned on the reaction layer substrate; the reaction layer substrate comprises a base, a three-electrode part prepared on the base and a contact metal pad connected with an external circuit, and the three-electrode part comprises a working electrode, a reference electrode and a counter electrode; a plurality of micro-channel grooves are formed in the bottom of the micro-channel sample adding layer, a plurality of up-down through inflow holes and outflow holes are formed in the micro-channel sample adding channel layer, and the two ends of each micro-channel groove are communicated with the inflow holes and the outflow holes respectively. The micro-fluidic chip is designed through photoetching, PVD and other technologies, the chip comprises a plurality of micro-channels, interference of the external environment to the detection process can be reduced through testing in a closed environment, and the micro-fluidic chip has the rapid, stable and high-throughput detection function and can be applied to bedside detection.

Described herein are immunoassay methods ("PRO-C17 assay") for measuring the extracellular domain levels of XVII collagen present in a patient sample, which can be used to detect and / or monitor cancer and / or assess the severity of cancer in a patient. Monoclonal antibodies and assay kits suitable for carrying out the methods are also described.

This disclosure provides an immunoassay with exceptional sensitivity for detecting a target antigen in a sample. In one disclosure, the capture and detection steps of such an assay utilize recombinant antibodies. The disclosed immunoassay may find specific applications in the detection of HBV antigen.

Disclosed are an amyloid β measurement method and a reagent capable of more accurately measuring amyloid β and unaffected by molecular target drugs (antibody drugs) for amyloid β. Provided is an immunoassay method for amyloid β in a sample isolated from a living body including a pretreatment step for mixing a sample isolated from a living body, (1) an acidifying agent, and (2) at least one selected from the group consisting of cationic surfactants, zwitterionic surfactants, protein denaturants, and chelating agents.

To provide a small, easy-to-operate electrochemiluminescence sensor. [Solution] An electrochemiluminescence sensor for detecting a test substance in a sample using nanoparticles with an electrochemiluminescence immunoassay method utilizing a plasmon-enhanced field, comprising: a first substrate; an electrode provided on the surface of the first substrate, wherein a first specific binding substance that specifically binds to the test substance is bound to the surface of the electrode; the nanoparticles comprising: metal nanoparticles; a polymer film covering the surface of the metal nanoparticles; a second specific binding substance bound to at least one of the polymer film and the metal nanoparticles and specifically binding to the test substance; and an electrochemiluminescent substance bound to at least one of the polymer film and the second specific binding substance and capable of contacting a co-reactant, wherein the electrochemiluminescence sensor comprises: a first substrate; an electrode provided on the surface of the first substrate, wherein a first specific binding substance that specifically binds to the test substance is bound to the surface of the electrode; and an electrochemiluminescent substance that is bound to at least one of the polymer film and the second specific binding substance and capable of contacting a co-reactant.

The presently described and claimed technology relates immunoassay methods of assessing a subject's Alzheimer's Disease status and zygosity for the APOE ε4 allele.

This invention belongs to the field of immunology and discloses a hybridoma cell line and the preparation and application of its secreted monoclonal antibody that uniformly recognizes tylosin / tilmicosin. The monoclonal antibody hybridoma cell line is named DES-AOAA-14D5, with accession number CGMCC NO: 45303. This invention utilizes decarboxymethyl tylosin and oxycarboxymethyl hydroxylamine to synthesize a hapten, conjugates a carrier protein to prepare a complete antigen, immunizes mice, and, through cell fusion and screening techniques, prepares a hybridoma cell line that can secrete a monoclonal antibody that uniformly recognizes tylosin / tilmicosin. IC50 50 The effective concentrations were 1.59 and 1.72 ng / mL, respectively, with a cross-reactivity rate as high as 92.44%. An immunoassay method for the simultaneous and accurate detection of tylosin / tilmicosin in milk was established using this antibody. The cut-off value for both tylosin and tilmicosin was 16 ng / mL, and no cross-reactivity was observed with other structural analogs. The detection method of this invention shows promising application prospects.

Provided is a heterohybridoma-based method of generating recombinant rabbit monoclonal antibodies, recombinant anti-IL-6 receptor-alpha (IL-6Rα) antibodies generated using such methods, and immune assay methods kits employing such antibodies.

The invention provides a linear epitope of an African swine fever virus CP312R protein. The amino acid sequence of the linear epitope is SEQ ID NO: 1 or SEQ ID NO: 2. The antigen epitope polypeptide provided by the invention has good antigen specificity and immunoreactivity. The polypeptide can be efficiently combined with an African swine fever virus specific antibody, background interference is low, cross reaction is small, and detection sensitivity and accuracy are remarkably improved. By using the polypeptide as a coating antigen in immunodetection methods such as ELISA or colloidal gold immunochromatography, rapid and accurate detection of ASFV infection can be realized, and the detection result is stable and reliable. Compared with a traditional whole virus antigen or a crude extract antigen, the epitope polypeptide has the advantages of being simple and convenient to prepare, small in batch difference, high in safety and the like, and is suitable for ASFV serological monitoring and diagnosis application.

The application discloses an antibody for detecting phosphorylated Tau protein pTau217, an immunodetection method and application. The antibody comprises a T217-3D6 antibody; light chain CDR1, CDR2 and CDR3 of the T217-3D6 antibody are sequentially the sequences shown in SEQ ID NO. 1 to 3, and heavy chain CDR1, CDR2 and CDR3 are sequentially the sequences shown in SEQ ID NO. 4 to 6. The antibody is used for immunodetection of the phosphorylated Tau protein pTau217, and has the advantages of simple operation, high sensitivity and strong specificity, and can realize rapid detection of the phosphorylated Tau protein pTau217, and has important significance for evaluating the Tau protein phosphorylation level and Tau protein pTau217 phosphorylation related detection.

This invention belongs to the field of molecular biology detection technology and is mainly used to solve the problem of large amounts of washing solution consumption caused by frequent rinsing in solid-phase immunoassay methods. Specifically, based on the characteristic that the artificial introduction of upstream reagents in solid-phase immunoassay methods will not affect downstream steps, the liquid in the upstream rinsing step is recovered and used for rinsing downstream steps. To avoid cross-binding of upstream analyte-specific reagents between different samples, the recovered washing solution is used for downstream rinsing using an instantaneous rinsing method to minimize the time spent in the process. When the entire batch of samples is testing for the same analyte, the recovered washing solution can be used for immersion washing in each rinsing step.

The present invention relates to immunoassay methods suitable for detecting and / or monitoring cancer in patients, immunoassay kits suitable for carrying out said methods, and antibodies suitable for use in said methods and kits.

This invention provides an immunoassay method comprising the following steps: S1: performing two parallel immunoassays on a sample containing the target molecule, recording the results of the two parallel immunoassays as the first and second values, respectively; S2: calculating the ratio of the first value to the second value; S3: determining the concentration of the target molecule in the sample. The assay method of this invention can solve the problem of HOOK effect samples, and the method is not limited by the detection range, capable of directly measuring up to 10... 6 High sample concentrations at the ng / ml level; good repeatability and fast detection speed.

An antibody for use in an immunoassay method, the antibody comprising an Fc region, wherein at least part of a glycan comprised in the Fc region has been removed.

Immunoassay methods for detecting HNE-generated fragments of the α3 or α4 chain of type IV collagen in a patient sample and their use for detecting and / or monitoring inflammatory bowel disease (IBD) or a particular level of severity of the disease in a patient are disclosed. Also disclosed are monoclonal antibodies and assay kits for use in the immunoassay methods.

The present invention provides nanoparticles that can increase the intensity of electrochemiluminescence. [Solution] A nanoparticle body comprising metal nanoparticles, a polymer film coating the surface of the metal nanoparticles, a specific binding substance bonded to at least one of the metal nanoparticles and the polymer film and specifically binding to the test substance in the sample, and an electrochemiluminescent substance bonded to at least one of the polymer film and the specific binding substance.

The application relates to a hapten for detecting ethoxyquin, a complete antigen and a use method thereof. The application discloses a hapten for detecting ethoxyquin, and then a complete antigen obtained by coupling ethoxyquin hapten with hemocyanin is used as an immunogen, and a complete antigen obtained by coupling ethoxyquin hapten with chicken egg white albumin is used as a coating agent. An indirect detection enzyme-linked immunoassay method for ethoxyquin is established. The half-inhibition concentration of the method for ethoxyquin is 0.96 ug / mL, the detection limit is 0.6 ug / mL, the linear detection range is 0.72 ug / mL-1.28 ug / mL, and the curve correlation coefficient is 0.996. The method is high in specificity and sensitivity, simple and fast, and has a wide application prospect in the detection of ethoxyquin residues in food. The method is very suitable for realizing rapid qualitative or quantitative detection of ethoxyquin in food by primary site personnel.

The invention discloses a high-sensitivity wide-spectrum specific antibody for resisting parathion, parathion-methyl and fenitrothion, the high-sensitivity wide-spectrum specific antibody comprises a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is as shown in SEQ ID NO: 2; the amino acid sequence of the light chain variable region coding gene is shown as SEQ ID NO: 4. The invention also provides an antibody expression plasmid. The obtained variable region sequence gene is respectively connected to an expression vector containing a heavy chain constant region gene and a light chain constant region gene, and a new recombinant full-length antibody is successfully obtained through an HEK293 (F) mammalian cell expression system. Based on the characteristics of stability and easy preservation of recombinant expression plasmids, the recombinant expression plasmids can be used for multi-batch repeated production of prohibited and limited organophosphorus pesticide recombinant antibodies for resisting parathion and the like; a stable and reliable core reaction reagent is provided for construction of an immunoassay method for multiple residues of organophosphorus pesticides such as parathion in a development environment and a food sample and development of a field screening product.

The application discloses a single-cell immunoblotting method based on quenchable aptamer probes and relates to the technical field of single-cell protein detection. The method comprises the following steps: S1, a single-cell suspension sample is settled in a microwell array on a composite hydrogel chip; S2, the composite hydrogel chip is placed in an electrophoresis tank, then a lysis solution is slowly poured into the tank, and incubation lysis is carried out; S3, after cell lysis, an electric field is applied to both ends of the chip, and proteins enter a gel coating layer on the surface of the chip under the action of the electric field and start electrophoretic separation; S4, after gel electrophoresis is completed, the chip is placed under an ultraviolet lamp for exposure, and the gel is taken out after exposure is completed; S5, the gel is placed in an aptamer probe solution which is targeted to target proteins, and is soaked and dyed, and the fluorescence signal intensity of the target protein molecules is measured under a laser confocal fluorescence microscope. The application can improve the detection sensitivity of target proteins and has fast imaging speed.

The present invention relates to methods of immunoassay for detecting a biomarker which is an MMP-cleaved fragment of collagen type-I and its use in detecting and / or monitoring diseases such as kidney disease and diseases of the cardiovascular system including the heart.

The preparation and evaluation method comprises the following steps: 1, extracting a heavy-chain DNA fragment and a light-chain DNA fragment, carrying out recombinant transformation by using an overlapping PCR method to construct an scFv gene, 2, constructing an expression vector, culturing the expression vector, carrying out sequencing identification to obtain an scFv bacterial solution, recombining the scFv bacterial solution with a mouse Fc fragment, and identifying a sequence to obtain an MN8 bacterial solution, the method comprises the following steps: 1, preparing an MN8 bacterial liquid, 2, extracting the MN8 bacterial liquid, 3, carrying out inoculated culture on the MN8 bacterial liquid to obtain an MN8 genetically engineered antibody bacterial liquid, 4, extracting MN8 genetically engineered antibody bacterial liquid plasmids and carrying out transfection expression purification, and 5, carrying out antibody titer and thermal stability determination on the MN8 genetically engineered antibody. The scFv single-chain antibody obtained by recombination according to the method has the advantages of small molecular weight, high penetrability and the like, the stability and the sensitivity of an immunoassay method of the scolosporins can be improved, and a certain basis is provided for rapid determination of the scolosporins.

The application discloses an immunological analysis method based on high-molecular amplification signal amplification and belongs to the technical field of biological medicines. The application combines protein-high-molecular conjugates with biological analysis together, and successfully develops a method for protein quantitative analysis. On the basis of taking silica microspheres as a solid-phase carrier material, target analysis antigen protein molecules are coated in advance, specific recognition reaction of antigen and antibody is utilized, antibody IgG-I macromolecules grafted with a photo initiator in advance are indirectly fixed on the surface of the microspheres, and a "competition" immunological detection method is constructed. The method is a rapid, simple, inexpensive and enzyme-free cascade amplification detection technology, and has a wide application prospect.

To provide an immunoassay method and immunoassay apparatus that can quantify the target substance in a sample even at low concentrations. [Solution] An immunoassay method comprising a container or substrate containing a sample including a target to be measured and a plurality of labeled particles carrying antibodies that can directly or indirectly bind to the target to be measured, wherein the container or substrate is irradiated with light after contact between the sample including the target to be measured and the plurality of labeled particles carrying antibodies that can directly or indirectly bind to the target to be measured, the light being irradiated into a region of the container or substrate containing the labeled particles, and the irradiated light having passed through the region of the container or substrate containing the labeled particles, wherein the wavelength of the irradiated light is a wavelength that causes Mie scattering by each of the plurality of labeled particles.

To provide a method, a reagent and a kit for accurately measuring N-terminal pro-brain natriuretic peptide (NT-proBNP) in a sample with high sensitivity.SOLUTION: In a latex agglutination immunoassay method for NT-proBNP in a sample, N-terminal pro-brain natriuretic peptide (NT-proBNP) in the sample is reacted with latex particles to which an antibody recognizing NT-proBNP or an antibody fragment thereof is bonded in an aqueous medium in the presence of a pyridinium derivative.SELECTED DRAWING: None

The invention relates to the technical field of gene editing, in particular to a preparation method and application of a monoclonal antibody with a nano luciferase label. A CRISPR / Cas9 gene editing technology is utilized, a nano luciferase gene is knocked into an Ig gene of a hybridoma cell secreting a monoclonal antibody to obtain a stable cell strain with a function of secreting the monoclonal antibody with the nano luciferase label, and then the monoclonal antibody with the nano luciferase label is prepared by utilizing the stable cell strain. According to the method provided by the invention, the sensitivity of an antigen immunoassay method can be improved from the perspective of signal labeling, and the detection limit is reduced.