77 results about "Immunoassay method" patented technology

The present invention is directed to assay methods and compounds and kits used in such assays.

The invention discloses a YOLO-Drop-based 3D printing droplet microfluidic immunoassay method, which organically integrates a high-precision microfluidic chip preparation technology, a droplet-level immunoreaction system and a fluorescence imaging acquisition and deep learning detection algorithm. And full-chain closed-loop processing from droplet generation, fluorescence signal acquisition, image recognition, result statistics to concentration quantitative analysis is realized. Through deep combination of chip physical parameters and model input features, the method not only significantly improves the accuracy and stability of droplet identification and concentration quantification, but also enhances the robustness of the algorithm under complex sample conditions through multi-scale feature fusion and a signal dual screening mechanism. Meanwhile, the optimized YOLO-Drop model supports embedded efficient reasoning, so that the whole analysis process has the characteristics of real-time performance and high throughput, and the method is suitable for rapid quantitative detection of various biomarkers.

The invention belongs to the technical field of immunodetection, and discloses a preparation and characterization method of a rabbit-derived single-chain antibody for detecting a liver cancer marker GPC3. The rabbit-derived single-chain antibody has the characteristics of small volume, high specificity, high stability and good sensitivity, and can be used in an immunoassay method for detecting a liver cancer marker GPC3; according to the method, a specific rabbit single-chain antibody is obtained through screening, expression and purification by using a phage display technology, a GPC3 antigen is coated on a solid-phase carrier, the rabbit single-chain antibody is used as a detection carrier, the binding force of the single-chain antibody and the antigen is identified, and the method is high in specificity, good in sensitivity and high in specificity. A novel single-chain antibody with high specificity and high sensitivity is provided for detection of GPC3 in serum.

Described herein are immunoassay methods for detecting and / or monitoring a cancer in a patient. In the method a biofluid sample from a patient is contacted with a monoclonal antibody that specifically binds to a C-terminal epitope of type XXVIII collagen, and the amount of binding between the monoclonal antibody and peptides in the sample is detected and determined.

The invention comprises a micro-fluidic chip and an immunodetection method. The micro-fluidic chip comprises a reaction layer substrate and a micro-channel sample adding layer positioned on the reaction layer substrate; the reaction layer substrate comprises a base, a three-electrode part prepared on the base and a contact metal pad connected with an external circuit, and the three-electrode part comprises a working electrode, a reference electrode and a counter electrode; a plurality of micro-channel grooves are formed in the bottom of the micro-channel sample adding layer, a plurality of up-down through inflow holes and outflow holes are formed in the micro-channel sample adding channel layer, and the two ends of each micro-channel groove are communicated with the inflow holes and the outflow holes respectively. The micro-fluidic chip is designed through photoetching, PVD and other technologies, the chip comprises a plurality of micro-channels, interference of the external environment to the detection process can be reduced through testing in a closed environment, and the micro-fluidic chip has the rapid, stable and high-throughput detection function and can be applied to bedside detection.

This disclosure provides an immunoassay with exceptional sensitivity for detecting a target antigen in a sample. In one disclosure, the capture and detection steps of such an assay utilize recombinant antibodies. The disclosed immunoassay may find specific applications in the detection of HBV antigen.

To provide a small, easy-to-operate electrochemiluminescence sensor. [Solution] An electrochemiluminescence sensor for detecting a test substance in a sample using nanoparticles with an electrochemiluminescence immunoassay method utilizing a plasmon-enhanced field, comprising: a first substrate; an electrode provided on the surface of the first substrate, wherein a first specific binding substance that specifically binds to the test substance is bound to the surface of the electrode; the nanoparticles comprising: metal nanoparticles; a polymer film covering the surface of the metal nanoparticles; a second specific binding substance bound to at least one of the polymer film and the metal nanoparticles and specifically binding to the test substance; and an electrochemiluminescent substance bound to at least one of the polymer film and the second specific binding substance and capable of contacting a co-reactant, wherein the electrochemiluminescence sensor comprises: a first substrate; an electrode provided on the surface of the first substrate, wherein a first specific binding substance that specifically binds to the test substance is bound to the surface of the electrode; and an electrochemiluminescent substance that is bound to at least one of the polymer film and the second specific binding substance and capable of contacting a co-reactant.

The presently described and claimed technology relates immunoassay methods of assessing a subject's Alzheimer's Disease status and zygosity for the APOE ε4 allele.

This invention belongs to the field of immunology and discloses a hybridoma cell line and the preparation and application of its secreted monoclonal antibody that uniformly recognizes tylosin / tilmicosin. The monoclonal antibody hybridoma cell line is named DES-AOAA-14D5, with accession number CGMCC NO: 45303. This invention utilizes decarboxymethyl tylosin and oxycarboxymethyl hydroxylamine to synthesize a hapten, conjugates a carrier protein to prepare a complete antigen, immunizes mice, and, through cell fusion and screening techniques, prepares a hybridoma cell line that can secrete a monoclonal antibody that uniformly recognizes tylosin / tilmicosin. IC50 50 The effective concentrations were 1.59 and 1.72 ng / mL, respectively, with a cross-reactivity rate as high as 92.44%. An immunoassay method for the simultaneous and accurate detection of tylosin / tilmicosin in milk was established using this antibody. The cut-off value for both tylosin and tilmicosin was 16 ng / mL, and no cross-reactivity was observed with other structural analogs. The detection method of this invention shows promising application prospects.

Provided is a heterohybridoma-based method of generating recombinant rabbit monoclonal antibodies, recombinant anti-IL-6 receptor-alpha (IL-6Rα) antibodies generated using such methods, and immune assay methods kits employing such antibodies.

The invention provides a linear epitope of an African swine fever virus CP312R protein. The amino acid sequence of the linear epitope is SEQ ID NO: 1 or SEQ ID NO: 2. The antigen epitope polypeptide provided by the invention has good antigen specificity and immunoreactivity. The polypeptide can be efficiently combined with an African swine fever virus specific antibody, background interference is low, cross reaction is small, and detection sensitivity and accuracy are remarkably improved. By using the polypeptide as a coating antigen in immunodetection methods such as ELISA or colloidal gold immunochromatography, rapid and accurate detection of ASFV infection can be realized, and the detection result is stable and reliable. Compared with a traditional whole virus antigen or a crude extract antigen, the epitope polypeptide has the advantages of being simple and convenient to prepare, small in batch difference, high in safety and the like, and is suitable for ASFV serological monitoring and diagnosis application.

This invention belongs to the field of molecular biology detection technology and is mainly used to solve the problem of large amounts of washing solution consumption caused by frequent rinsing in solid-phase immunoassay methods. Specifically, based on the characteristic that the artificial introduction of upstream reagents in solid-phase immunoassay methods will not affect downstream steps, the liquid in the upstream rinsing step is recovered and used for rinsing downstream steps. To avoid cross-binding of upstream analyte-specific reagents between different samples, the recovered washing solution is used for downstream rinsing using an instantaneous rinsing method to minimize the time spent in the process. When the entire batch of samples is testing for the same analyte, the recovered washing solution can be used for immersion washing in each rinsing step.

This invention provides an immunoassay method comprising the following steps: S1: performing two parallel immunoassays on a sample containing the target molecule, recording the results of the two parallel immunoassays as the first and second values, respectively; S2: calculating the ratio of the first value to the second value; S3: determining the concentration of the target molecule in the sample. The assay method of this invention can solve the problem of HOOK effect samples, and the method is not limited by the detection range, capable of directly measuring up to 10... 6 High sample concentrations at the ng / ml level; good repeatability and fast detection speed.

Immunoassay methods for detecting HNE-generated fragments of the α3 or α4 chain of type IV collagen in a patient sample and their use for detecting and / or monitoring inflammatory bowel disease (IBD) or a particular level of severity of the disease in a patient are disclosed. Also disclosed are monoclonal antibodies and assay kits for use in the immunoassay methods.

The present invention provides nanoparticles that can increase the intensity of electrochemiluminescence. [Solution] A nanoparticle body comprising metal nanoparticles, a polymer film coating the surface of the metal nanoparticles, a specific binding substance bonded to at least one of the metal nanoparticles and the polymer film and specifically binding to the test substance in the sample, and an electrochemiluminescent substance bonded to at least one of the polymer film and the specific binding substance.

The application discloses a single-cell immunoblotting method based on quenchable aptamer probes and relates to the technical field of single-cell protein detection. The method comprises the following steps: S1, a single-cell suspension sample is settled in a microwell array on a composite hydrogel chip; S2, the composite hydrogel chip is placed in an electrophoresis tank, then a lysis solution is slowly poured into the tank, and incubation lysis is carried out; S3, after cell lysis, an electric field is applied to both ends of the chip, and proteins enter a gel coating layer on the surface of the chip under the action of the electric field and start electrophoretic separation; S4, after gel electrophoresis is completed, the chip is placed under an ultraviolet lamp for exposure, and the gel is taken out after exposure is completed; S5, the gel is placed in an aptamer probe solution which is targeted to target proteins, and is soaked and dyed, and the fluorescence signal intensity of the target protein molecules is measured under a laser confocal fluorescence microscope. The application can improve the detection sensitivity of target proteins and has fast imaging speed.

The present invention relates to methods of immunoassay for detecting a biomarker which is an MMP-cleaved fragment of collagen type-I and its use in detecting and / or monitoring diseases such as kidney disease and diseases of the cardiovascular system including the heart.

The preparation and evaluation method comprises the following steps: 1, extracting a heavy-chain DNA fragment and a light-chain DNA fragment, carrying out recombinant transformation by using an overlapping PCR method to construct an scFv gene, 2, constructing an expression vector, culturing the expression vector, carrying out sequencing identification to obtain an scFv bacterial solution, recombining the scFv bacterial solution with a mouse Fc fragment, and identifying a sequence to obtain an MN8 bacterial solution, the method comprises the following steps: 1, preparing an MN8 bacterial liquid, 2, extracting the MN8 bacterial liquid, 3, carrying out inoculated culture on the MN8 bacterial liquid to obtain an MN8 genetically engineered antibody bacterial liquid, 4, extracting MN8 genetically engineered antibody bacterial liquid plasmids and carrying out transfection expression purification, and 5, carrying out antibody titer and thermal stability determination on the MN8 genetically engineered antibody. The scFv single-chain antibody obtained by recombination according to the method has the advantages of small molecular weight, high penetrability and the like, the stability and the sensitivity of an immunoassay method of the scolosporins can be improved, and a certain basis is provided for rapid determination of the scolosporins.

The application discloses an immunological analysis method based on high-molecular amplification signal amplification and belongs to the technical field of biological medicines. The application combines protein-high-molecular conjugates with biological analysis together, and successfully develops a method for protein quantitative analysis. On the basis of taking silica microspheres as a solid-phase carrier material, target analysis antigen protein molecules are coated in advance, specific recognition reaction of antigen and antibody is utilized, antibody IgG-I macromolecules grafted with a photo initiator in advance are indirectly fixed on the surface of the microspheres, and a "competition" immunological detection method is constructed. The method is a rapid, simple, inexpensive and enzyme-free cascade amplification detection technology, and has a wide application prospect.

To provide an immunoassay method and immunoassay apparatus that can quantify the target substance in a sample even at low concentrations. [Solution] An immunoassay method comprising a container or substrate containing a sample including a target to be measured and a plurality of labeled particles carrying antibodies that can directly or indirectly bind to the target to be measured, wherein the container or substrate is irradiated with light after contact between the sample including the target to be measured and the plurality of labeled particles carrying antibodies that can directly or indirectly bind to the target to be measured, the light being irradiated into a region of the container or substrate containing the labeled particles, and the irradiated light having passed through the region of the container or substrate containing the labeled particles, wherein the wavelength of the irradiated light is a wavelength that causes Mie scattering by each of the plurality of labeled particles.

The invention provides a method for detecting the protein state of gene-edited cynoglossus semilaevis Dmrt1, namely a protein immunodetection method based on an antigen and an antibody thereof is established on the basis of designing and obtaining optimized antigen polypeptide capable of specifically recognizing gene-edited derived protein, so that accurate identification and product monitoring of the gene-edited cynoglossus semilaevis are realized. The amino acid sequence of the antigen polypeptide is SEQ ID NO: 2. The optimized antigen capable of specifically recognizing a gene editing product is obtained through design and is used for preparing a high-titer antibody and developing a detection product, so that a method for directly and accurately identifying the gene editing cynoglossus semilaevis at the protein level is established, the defect that the edited protein product cannot be detected in the prior art is overcome, and the detection efficiency is improved. And a key tool is provided for functional verification and biological safety evaluation of the gene editing fish.

The invention relates to the technical field of biological medicines, and relates to specific primers and a method for identifying coral snake, cobra, king cobra and snake venom source species thereof, and application of the specific primers and the method. Based on highly-conservative and specific DNA fragments contained in DNAs extracted from tissues and snake venom of bungarus multicinctus, cobra and king cobra, specific primers are respectively designed for the fragments, and the bungarus multicinctus, cobra and king cobra and snake venom source species can be accurately identified. The method can be used for identifying bungarus multicinctus, cobra, king cobra and snake venom source species of bungarus multicinctus, cobra, king cobra and snake venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus The method is high in detection sensitivity, high in correct detection rate, free of cross reaction and simple in operation process, and has higher specificity compared with a traditional immunoassay method.

This invention discloses an immunoassay method for detecting HNE-producing fragments of club cell secreted protein 16 (CC16) in patient samples, which can be used to detect and / or monitor chronic obstructive pulmonary disease (COPD) or idiopathic pulmonary fibrosis (IPF) or a specific level of severity of these diseases in patients. Also disclosed are a monoclonal antibody and an assay kit for use with this immunoassay method.

To provide a method for improving cross-reactivity, that is, improving specificity in an immunoassay method using anti-immunocomplex antibody.SOLUTION: A method of immunoassay includes the following steps (i) to (iii): (i) reacting an anti-hapten rabbit monoclonal antibody indirectly immobilized on a water-insoluble carrier with a hapten (for example, digoxin) in a solution to be measured; (ii) reacting a labeled anti-immunocomplex mouse monoclonal antibody with a hapten-anti-hapten antibody immune complex; and (iii) detecting a signal derived from the label.SELECTED DRAWING: Figure 2

Disclosed herein are immunoassays for detecting anti-thyroid peroxidase antibodies in a biological sample from a subject and / or diagnosing a thyroid disease in a subject. The disclosed immunoassays employ recombinant thyroid peroxidase (rTPO) and anti-TPO monoclonal antibodies to assess the level of anti-thyroid peroxidase antibody-induced formation or disruption of complexes comprising a solid support and the rTPO. Also disclosed are methods and kits thereof.

The application discloses a DNA tetrahedron space-mediated immunoassay method and application thereof in detection of imidacloprid residues, relates to the field of pesticide detection, and realizes spatial ordered arrangement of target antibodies by regulating the spatial density of target antibodies through coupling target antibodies on magnetic beads in the form of DNA tetrahedrons, so that a detection probe of MB@TDN@Ab is formed, trace detection of a to-be-detected substance can be realized, and high sensitivity and accuracy are achieved. Compared with a traditional magnetic separation immunoassay method, the detection sensitivity of the application is improved by about 2 times, and the linear range is improved by 1 order of magnitude. When the application is applied to detection of pesticides such as imidacloprid, it has important significance for guaranteeing food safety.

To provide a monoclonal antibody, an adenovirus immunoassay method using same, and an immunoassay device capable of detecting and measuring adenovirus contained in a test sample quickly, simply, and with high sensitivity.SOLUTION: The present invention provides a monoclonal antibody or an antigen-binding fragment thereof that undergoes an antigen-antibody reaction with a polypeptide containing the amino acid sequence from position 21 to 944 of the amino acid sequence indicated in SEQ ID NO: 1, an immunoassay method and an immunoassay device using same.SELECTED DRAWING: Figure 1