Whole-blood labeled immunoassay method and instant detection system

An immunoassay and detection system technology, which is applied in the direction of analyzing materials, analyzing materials through chemical reactions, measuring devices, etc., can solve the problems of low sensitivity, stability and repeatability, and achieve repeatability and stability Low, high sensitivity, good repeatability

Active Publication Date: 2014-02-12
SUZHOU HUAMAI XINGWEI MEDICAL TECH +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0008] The technical problem to be solved by the present invention is that the current real-time retrieval method has low sensitivity, stability and repeatability. In order to overcome the above deficiencies, a rapid immune analysis method and real-time detection system for whole blood are provided

Method used

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  • Whole-blood labeled immunoassay method and instant detection system
  • Whole-blood labeled immunoassay method and instant detection system
  • Whole-blood labeled immunoassay method and instant detection system

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Embodiment Construction

[0038] The present invention is described in detail below in conjunction with accompanying drawing and embodiment:

[0039] The source of sample, reaction reagent and detection instrument in the embodiment is as follows:

[0040] Whole blood samples: 30 blood samples with prostate-specific antigen (PSA), the antigen concentrations are 0pg / ml, 5pg / ml, 10pg / ml, 50pg / ml, 150pg / ml, 500pg / ml, 1.5ng / ml , 5ng / ml, 50ng / ml and 150ng / ml, 3 samples for each concentration, whole blood samples were added with different amounts of prostate-specific antigen (PSA) standard (R&D Systems, USA) (EDTA) obtained from healthy human blood after anticoagulant treatment. Available anticoagulants also include citric acid, heparin, and the like.

[0041] The magnetic beads loaded with the capture antibody used in the method or system are NanoLink from the US solulink company. TM Streptavidin Magnetic Beads (about 0.8μm in diameter) and MagnaLink TM Streptavidin Magnetic Beads (about 2.8 μm in diam...

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Abstract

The invention in particular relates to a whole-blood labeled immunoassay method for quickly collecting a target object from whole-blood through specificity binding reaction and labeling and monitoring the target object by a labeling technology, and an instant detection system. The method comprises the following steps: 1) capturing magnetic beads; 2) eluting blood; 3) labeling a detection antibody; 4) eluting the detection antibody; 5) detecting. The system comprises a microfluidic chip, a magnetic field generation device arranged above or below the microfluidic chip and a detection device, wherein the microfluidic chip comprises a microchannel, the magnetic beads arranged in the microchannel, a waste liquid outlet and an eluent storage pond; the microchannel is separated into a first reaction region, a second reaction region and a detection region in stages by a separation mechanism. The technical scheme provided by the invention has the characteristics of high sensitivity, high repeatability, simple system structure, low cost and the like, and can detect the target object from trace blood samples quickly and quantitatively.

Description

technical field [0001] The invention belongs to rapid labeling immunoassay technology, in particular to a method for rapidly collecting a target object from whole blood through a specific binding reaction, marking and monitoring the target object through a labeling technique, and a real-time detection system. Background technique [0002] Labeled immunology technology has the advantages of high selectivity, high sensitivity, and good universality, mainly including radioimmunotechnology, enzyme immunology technology, fluorescence immunology technology, chemiluminescence immunology technology, using tracer substances such as fluorescein, isotope or enzyme to label antibodies (or antigen) for antigen-antibody reaction, and through the determination of the markers in the immune complex, the purpose of monitoring the immune response is achieved. [0003] Point-of-care testing (POCT) refers to a type of method that analyzes immediately at the sampling site, saves the complicated p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N21/76
CPCG01N33/537G01N33/54333G01N33/54366
Inventor 施奇惠司珂
Owner SUZHOU HUAMAI XINGWEI MEDICAL TECH
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