48 results about "Fluorescein" patented technology

This invention discloses an AfBPP-based active molecular probe for thiostreptin, its preparation, and its application, belonging to the field of chemical biology. This invention introduces a bifunctional tag integrating a photocrosslinking group (bisacrididine) and a bioorthogonal reactive group (alkynyl group) into the structure of thiostreptin. While retaining the original biological activity of the parent compound, it endows the probe with highly efficient probe function, solving the technical problem of target loss during washing and purification of traditional non-covalent probes. After target labeling, the probe can specifically connect to reporter groups such as fluorescein or biotin through click-chemical reactions, achieving efficient enrichment of drug targets. Combined with mass spectrometry analysis, it enables global identification of potential targets in cells or complex biological samples at the omics level, such as chemical proteomics, providing a powerful molecular tool for in-depth revelation of the potential targets and pharmacological mechanisms of thiostreptin.

The utility model discloses quick-drying type thermal dye sublimation transfer printing body paper which comprises an antistatic layer, a thermal dye sublimation layer fixedly connected to the lower surface of the antistatic layer, a water absorption layer fixedly connected to the lower surface of the thermal dye sublimation layer, pores formed in the outer surface of the water absorption layer, a filler layer fixedly connected to the lower surface of the water absorption layer, and an anti-static layer fixedly connected to the filler layer. The lower surface of the filler layer is fixedly connected with the aluminum foil layer, the lower surface of the aluminum foil layer is fixedly connected with the body paper, moisture is absorbed through the water absorption layer, meanwhile, pores are formed in the surface of the water absorption layer, rapid drying is achieved, the drying time is shortened, the filler layer is further arranged between the water absorption layer and the body paper, and coating moisture is prevented from permeating into the body paper layer; humidity-sensitive fluorescein of the humidity-sensitive layer displays different colors under the condition of high and low humidity, so that people can directly observe the humidity of the body paper, and meanwhile, the hydrophobic layer is arranged on the surface of the humidity-sensitive layer, so that the influence of external moisture is reduced.

The invention provides a specific probe for detecting perfluorinated compounds in a water body and a detection method thereof, and belongs to the technical field of detection. The probe provided by the invention is a compound formed by cucurbituril and fluorescein; the detection method provided by the invention comprises the following steps: adding a cucurbituril and fluorescein conjugate into a to-be-detected water sample containing the perfluorinated compounds, carrying out equilibrium reaction for a period of time, and then detecting the fluorescence response intensity in the water body so as to quantitatively evaluate the concentration of the specific perfluorinated compounds in the water sample. Compared with the prior art, the probe equipment provided by the invention is small in size, convenient to carry, capable of simultaneously detecting multiple samples and short in reaction time; the whole material of the probe is green and harmless, and chemical agents such as sulfate which easily cause secondary pollution are not used; other impurity removal and enrichment small columns and complex masking agents do not need to be used. In addition, the detection method provided by the invention has a lower detection limit, the sensitivity and convenience of novel perfluorinated compound detection are remarkably improved, and the application range is wider.

The invention relates to novel biocompatible hybrid nanoparticles of very small size, useful in particular for diagnostics and / or therapy.The purpose of the invention is to offer novel nanoparticles which are useful in particular as contrast agents in imaging (e.g. MRD and / or in other diagnostic techniques and / or as therapeutic agents, which give better performance than the known nanoparticles of the same type and which combine both a small size (for example less than 20 nm) and a high loading with metals (e.g. rare earths), in particular so as to have, in imaging (e.g. MRI), strong intensification and a correct response (increased relaxivity) at high frequencies.Thus, the nanoparticles according to the invention, with diameter d1 between 1 and 20 nm, each comprise a polyorganosiloxane (POS) matrix including gadolinium cations optionally associated with doping cations; a chelating graft C1 DTPABA (diethylenetriaminepentaacetic acid bisanhydride) bound to the POS matrix by an —Si—C— covalent bond, and present in sufficient quantity to be able to complex all the gadolinium cations; and optionally another functionalizing graft Gf* bound to the POS matrix by an —Si—C— covalent bond (where Gf* can be derived from a hydrophilic compound (PEG); from a compound having an active ingredient PA1; from a targeting compound; from a luminescent compound (fluorescein).The method for the production of these nanoparticles and the applications thereof in imaging and in therapy also form part of the invention.

The application belongs to the technical field of food safety rapid detection, and discloses a fluorescence and colorimetric dual-signal biosensor for acetamiprid detection, a preparation method and application thereof.The application uses aptamer (Apts) functionalized Fe3O4@Au@Pt (Fe3O4@Au@Pt@Apt) and fluorescence-labeled (FAM, 6-carboxyfluorescein) aptamer complementary strand (cDNA) functionalized SiO2 (SiO2@cDNA-FAM) as a recognition probe, uses specific recognition of the aptamer to a target, and uses enzyme catalytic activity of Fe3O4@Au@Pt and quenching effect of the fluorescence, and cooperates with a magnetic separation technology to construct a colorimetric and fluorescence dual-signal output high-sensitivity aptamer sensor for acetamiprid detection.

The present invention relates to a method for treating Gram-negative bacteria that are resistant to colistin (MIC > 50 mg / mL), an antibacterial compound, excluding those of the genera Burkholderia, Proteus, and Serratia, comprising contacting the bacteria with an aqueous pharmaceutical composition containing a rose bengal (RB) compound of formula I, dissolved or dispersed at a concentration of about 0.01 to about 15 mg / mL, and irradiating the contacted bacteria with light of a wavelength of about 500 nm to about 600 nm for a period of about 1 to about 10 minutes, at a concentration of about 16 to about 160 J / cm². 2 The aim is to create a method that involves obtaining a certain amount of light to treat and kill irradiated bacteria. [Formula 1] JPEG2026511692000028.jpg5864 (where X, R 1 , R 2 (and M+ are defined herein)

The present application relates to a kind of HER2 gene and FGFR2 gene fluorescence in situ hybridization combined detection probe and its application, belong to biological medicine technical field.The present application provides a kind of probe and probe composition for tumor diagnosis and prognosis evaluation, including the first probe for detecting HER2 gene, and the second probe and the third probe for detecting FGFR2 gene;First probe, second probe and third probe are respectively marked with the first fluorochrome, second fluorochrome and third fluorochrome that can produce different colors;By the present application, a kind of fluorescence in situ hybridization combined detection method and its kit for simultaneously detecting HER2 and FGFR2 gene variation are provided;Wide application range can be used in various tissue and cell samples needing simultaneously detecting HER2 and FGFR2 gene state, one detection, two results, meet the goal of clinical test quality improvement and efficiency improvement;It has quite extensive application prospect.

Systems for customized visual field (VF) testing use machine learning models (15) trained on retinal images (12A, 12C, 12D) including optical coherence tomography (OCT), optical coherence tomography angiography (OCTA), fundus, and / or fluorescein angiography images. In operation, when a particular VF test (13) is to be prepared for a patient, a retinal image of the patient is submitted to the current machine model, which responds by synthesizing a VF for the patient. The synthesized VF can be used to optimize the particular VF test prior to performing the particular VF test on the patient.

The present invention contemplates a topical ophthalmic system for treating a Gram-positive bacterially-infected mammalian eye. The system comprises an ophthalmic composition containing a halogenated fluorescein or a pharmaceutically acceptable salt or ester thereof dissolved or dispersed in an aqueous ophthalmic carrier and present in an anti-bacterial keratitis-treating effective concentration of about 0.2 μg / mL to about 50 μg / mL. The ophthalmic composition has a pH value of about 6.5 to about 7.6, a viscosity of about 10 to about 300 cps and an osmolality of about 270 mOsm / kg to about 340 mOsm / kg. The ophthalmic composition is present in a vessel opaque to actinic light. Also contemplated is a method of treating a mammal having a Gram-positive bacterial infection of an eye by administering the ophthalmic composition to the infected eye and maintaining the treated eye in the substantial absence of actinic light for about 3 hours to about 12 hours.

The application discloses a curcumin photoaffinity probe molecule and a preparation method and application thereof, and the preparation method comprises the following steps: taking curcumin (compound II) as raw material, and reacting with 3-but-3-alkynyl-3-(2-iodoethyl) diazole alkene (compound III) under alkaline conditions to obtain the curcumin photoaffinity probe molecule (compound I), wherein a photo-crosslinking group and a biological orthogonal group are introduced at the 4th position of the curcumin photoaffinity probe molecule, the diazirine photo is the crosslinking group, can in-situ crosslink the adjacent binding protein under the condition of ultraviolet light, the alkynyl group is the biological orthogonal group, and the probe is coupled with fluorescein or biotin through a biological orthogonal coupling reaction, so that the probe is used for fluorescence detection on curcumin target protein in cells and identification of the curcumin target protein.

The invention relates to the technical field of wafer COP defect identification methods based on detection before polishing, particularly discloses a wafer COP defect identification method based on detection before polishing, and aims to solve the problems of high invalid processing cost and low screening accuracy caused by the fact that traditional COP defect identification depends on a polished surface. The method comprises the following steps: carrying out alkali corrosion treatment on a silicon wafer to expose crystal original particle defects; selectively marking the defect area by adopting a fluorescein aqueous solution with specific wavelength and concentration; after cleaning with high-purity water and drying with compressed air, analyzing the peak position, the peak width and the strength of a defect luminescence peak through photoluminescence spectrum, and realizing accurate identification of the defect by combining a support vector machine model. By adopting the technical scheme, the wafer COP defect can be efficiently and accurately identified before polishing, the invalid polishing cost is remarkably reduced, and the process economy and the detection flux of regenerated wafer screening are improved.

The application belongs to the field of fluorescent probes, and relates to a lysosome-targeting fluorescent probe and a preparation method and application thereof. An intermediate is prepared by mixing an aldehyde compound, 5-amino fluorescein and 7-bromoheptanoic acid, and then performing a reaction; the intermediate is mixed with morpholine, and then a reaction is performed, so that the lysosome-targeting fluorescent probe is obtained. The application belongs to the construction of a complex molecular system with flexible and modular Ugi four-component reaction, adopts a one-pot method, and is synthesized by a framework template conjugation method, and the yield of the product is between 60% and 80%. The fluorescent probe prepared in the application can target lysosomes, and can be used as a lysosome-targeting imaging agent for living cancer cells. The fluorescent probe containing ferrocene prepared in the application can target lysosomes, and can be used as a lysosome-targeting imaging agent and a therapeutic agent for living cancer cells. The fluorescent probe containing ferrocene prepared in the application can effectively inhibit the growth of Hela and other cancer cells.

The invention discloses an oil spill degradation intermediate product in-situ detection kit and a use method thereof, and belongs to the field of environmental monitoring, the kit comprises an alkane degradation indication module, an aromatic hydrocarbon degradation indication module and a total petroleum hydrocarbon residue detection module, the alkane degradation indication module utilizes fatty alcohol dehydrogenase to catalyze a reaction between fatty alcohol and NAD, and the total petroleum hydrocarbon residue detection module is used for detecting total petroleum hydrocarbon residues. NADH reduces tetrazole salt to generate a purple formazan compound for colorimetric detection, and the aromatic hydrocarbon degradation indication module utilizes catechol dioxygenase to catalyze ring opening of catechol to generate a yellow semialdehyde compound for colorimetric detection. According to the total petroleum hydrocarbon residue detection module, fluorescence quenching is caused by an inclusion compound formed by fluorescein labeled cyclodextrin and petroleum hydrocarbon, the kit is matched with a portable photometer and data processing software, detection can be completed within 15 minutes on site, an evaluation index of the oil spill degradation efficiency can be calculated, the detection sensitivity reaches 5 milligrams per liter, and the detection time is short. And a rapid screening tool is provided for oil spill bioremediation engineering.

This invention proposes a microfluidic chip and its application based on dual-color and dual-layer technology to increase detection sites. The chip includes a substrate and a cover plate laminated onto the substrate. The substrate and cover plate enclose a microchannel. The microchannel is provided with a labeling region and a detection region. The labeling region includes a first labeling region on the lower surface of the cover plate and a second labeling region on the upper surface of the substrate. The detection region includes multiple detection sites on the lower surface of the cover plate and the upper surface of the substrate. The first labeling region is coated with a first labeled antigen / antibody, and the second labeling region is coated with a second labeled antigen / antibody. The first and second labeled antigens / antibodies are fluorescent microspheres labeled with two fluorophores that have the same excitation spectrum but different emission spectra. The multiple detection sites are respectively coated with capture antigens / antibodies that bind to different target analytes. By using a layered design combined with dual-color labeling, the number of simultaneously detectable indicators within the microchannel is increased.

The invention relates to the technical field of luminescent materials, in particular to an organic-phosphate hybrid afterglow luminescent material with visible light excitation performance as well as a preparation method and application of the organic-phosphate hybrid afterglow luminescent material. The organic-phosphate hybrid afterglow luminescent material is prepared by mixing phosphate and fluorescein or a derivative thereof, heating, and cooling to room temperature. The preparation method is simple to operate, and the obtained afterglow composite material is excellent in luminescence property. The luminescent material can be used as a temperature sensing label, a characteristic luminescent material in latent fingerprint imaging identification or biological tissue (such as muscular tissue or gastric mucosa) imaging identification process.

The invention discloses a method for rapidly detecting seed activity and application, FDA has no fluorescence and is hydrolyzed by seed esterase to generate fluorescein, so that a fluorescence signal is specifically generated. The seed vigor is detected through the FDA probe, the detection time is remarkably shortened, the detection sensitivity and accuracy are improved, high-throughput detection is achieved, and a set of efficient, accurate and large-scale technical scheme is provided for large-scale seed vigor screening in the agricultural field.

Methods and systems are provided to enable multiple imaging modalities with a single camera head. The camera head may include a two chip or a three chip system capable of measuring different wavelengths of light to generate different images. The light source in the system can adjust the output spectra, by using a plurality of illuminants and filters, and measurements from different light channels on one or more images sensors can be used to generate white light images, fluorescence image, perfusion images, fluorescein images, and the like.

This specification describes a dental imaging and / or curing system and methods of using it, optionally in combination with a fluorescent imaging aid applied to a tooth. In some examples, a system or kit described in this specification combines a fluoresecent compound with an intra-oral device. The intra-oral device includes a light source to excite the fluorescent compound. The intra-oral device further includes a sensor to produce an image of fluorescent light emitted from the fluorescent compound. Optionally, the fluorescent compound can include positively charged nanoparticles including a fluorophore, for example fluorescein. Optionally, the intra-oral device can include a blue LED, a bandpass emission filter and a digital camera sensor. This specification also describes an intra-oral device and a method of producing an image of plaque, calculus or active carious lesions in the mouth of a person or other animal.

The method for self-sensitization detection of 2-chlorophenol based on eosin Y-carbon nitride system relates to the application technology field of eosin Y-carbon nitride system in visual detection of 2-chlorophenol. The purpose of the present application is to solve the problems of how to improve the selective adsorption capacity of g-C3N4 to trace concentration 2-chlorophenol and realize visual super-sensitive detection of colorless 2-chlorophenol. Method: tetra bromo fluorescein disodium aqueous solution, silver modified carbon nitride nanosheet suspension and 2-chlorophenol aqueous solution are mixed, and the pH is adjusted to 3-9; the mixed solution is placed in a photocatalytic reactor, stirred and adsorbed for 1-30 min in the dark, 525 nm visible light is turned on, and after light irradiation, the filtrate is detected by ultraviolet visible spectrophotometer, and the visual detection of 2-chlorophenol in water based on single-atom silver modified carbon nitride nanosheet is completed. The method for self-sensitization detection of 2-chlorophenol based on eosin Y-carbon nitride system can be obtained.

The present application relates to a kind of C-MET gene and EGFR gene fluorescence in situ hybridization combined detection probe and its application, belong to biological medicine technical field.The present application provides a kind of probe and probe composition for tumor diagnosis and prognosis evaluation, including the first probe for detecting C-MET gene, and the second probe for detecting EGFR gene, the first probe and the second probe are labeled with the first fluorescein and the second fluorescein that can produce different colors respectively;By the present application, a kind of fluorescence in situ hybridization combined detection method and its kit for simultaneously detecting C-MET gene and EGFR gene gene variation are provided;Wide application range can be used in various tissue and cell samples needing simultaneously detecting C-MET gene and EGFR gene state, multiple results are detected once, meet the goal of clinical test quality improvement and efficiency improvement;It has quite extensive application prospect in the prognosis of various cancers.

A multi-functional background illuminator for use with slit lamps is disclosed. The illuminator includes a housing with non-filtered green, blue, and white light-emitting diodes, a rechargeable power supply, an electronic control board, and a rheostat for intensity control. A multi-directional mounting clip attaches the illuminator to a slit lamp tower and permits horizontal and vertical rotation. The green LED enhances visualization of meibomian glands without infrared imaging, the blue LED provides an expanded excitation field for fluorescein dye to improve assessment of tear film and corneal staining, and the white LED supplies wide-field diffused illumination with reduced glare for examination of eyelids, lashes, and conjunctival surfaces. The device extends the diagnostic capabilities of conventional slit lamps by providing wider field illumination and multiple selectable lighting modes in a compact, portable unit.

Methods and systems are provided to enable multiple imaging modalities with a single camera head. The camera head may include a single chip system capable of measuring different wavelengths of light on different regions of the same image sensor to generate different images. The light source in the system can adjust the output spectra by using a plurality of illuminants and filters, and measurements from different light channels from different regions of the image sensor can be used to generate white light images, fluorescence image, perfusion images, fluorescein images, and the like.

The application discloses application of a fluorescein substance in latent blood trace development. The fluorescein substance is a compound shown in formula I or formula II. Based on the fluorescein substance, the application provides a latent blood trace developing agent which is a solution formed by the fluorescein substance and any one of the following solvents: water, methanol, ethanol, ethyl acetate, DMSO and DMF. The pH value of the solution is 3-5, preferably 4.3-4.9. The development method of the application is suitable for development of latent blood traces on various non-penetrating objects, has a wide range of applicable objects, has a good development effect on latent blood traces on dark objects and rough objects in particular, has strong fluorescence brightness of the lines, the lines are relatively clear and coherent, and the lines have a large contrast with the background.

The application discloses a method for improving the stability of freeze-dried fluorescent microsphere-labeled antibody, which comprises the following steps: S1, mixing polystyrene microsphere solution containing fluorescent dye, EDC solution and NHS solution to perform activation reaction, and obtaining activated microsphere solution; S2, adding antibody solution into the activated microsphere solution to perform coupling reaction, and obtaining coupling reaction liquid; S3, adding microsphere blocking solution into the coupling reaction liquid to perform blocking reaction, then performing centrifugation to remove supernatant, then adding microsphere washing solution to perform cleaning, then performing centrifugation to remove supernatant, and obtaining blocked microspheres; and S4, adding microsphere storage solution into the blocked microspheres, and uniformly mixing by ultrasonic to obtain microsphere storage solution, which is stored at 2-8 DEG C in dark for standby use. The microsphere storage solution composite formula developed by the application can effectively prevent the destruction of antibody structure in the freeze-drying process, meanwhile, the stability of fluorescein is maintained, the activity retention rate of the antibody and the fluorescence intensity are significantly improved, and the shelf life of the product is prolonged.

The invention relates to the technical field of polymer degradation, and discloses a PLA degradation degree determination method and application. The determination method comprises the following steps: (1) preparing a PLA-FDL film, wherein the PLA-FDL film is a PLA film embedded with fluorescein dilaurate; (2) carrying out mixed reaction on PLA hydrolase and the PLA-FDL membrane; and measuring the fluorescence value of the product in the reaction process. According to the determination method, real-time and nondestructive monitoring of the PLA degradation process can be realized, degradation kinetics of enzymes or microorganisms can be dynamically reflected, the process is simple, operation is simple and convenient, and the determination method has a remarkable application prospect.

The application discloses a method for detecting aortic dissection based on peripheral blood BRD4 by using flow cytometry and application thereof. The application belongs to the technical field of biology and particularly relates to a method for detecting aortic dissection based on peripheral blood BRD4 by using flow cytometry and application thereof. The method for detecting the content or expression level of bromodomain-containing protein 4 in a to-be-detected sample established by the application comprises the following steps: 1) processing a to-be-detected sample to obtain single nucleus cells; 2) determining a fluorescein-coupled antibody composition of a detection index according to a flow cytometry color matching scheme; 3) mixing the single nucleus cells in step 1) and the fluorescein-coupled antibody in step 2) uniformly, and performing staining by incubation in the dark; 4) performing sample testing on a spectrum flow cytometer; and 5) performing data result analysis. The detection index in step 2) is CD45, CD11B, CD66B, CD14, CC16 and BRD4.

The invention discloses a pollutant on-line monitoring method, and belongs to the technical field of on-line monitoring. According to the method, fluorescent dyes including but not limited to coumarins, fluorescein, naphthalimides and rhodamine are subjected to silanization and prepared on a substrate including but not limited to quartz, high borosilicate glass, ITO glass, polystyrene (PS), polymethyl methacrylate (PMMA) and the like, a flowing water phase passes through the substrate, the fluorescent dyes are excited through an excitation light source, and the fluorescent dyes are subjected to fluorescence detection, so that the fluorescent dyes are obtained. And the fluorescence intensity and wavelength are detected by using an optical sensing detection technology, so that high-sensitivity online monitoring of pollutants is realized.

Systems and methods for detecting and classifying retinal microaneurysms. The method including: receiving a time sequence of fluorescein angiography input images; generating a binary map of hyperfluorescent elements in the input images; determining which hyperfluorescent elements in the binary map are microaneurysms, by grading each against a combination of morphological metrics; classifying each of the detected microaneurysms as leaky or not leaky, the classification having: identifying an outer ring mask surrounding the detected microaneurysm in the binary map; identifying parenchyma in the outer ring mask using a fluorescence intensity determination; determining a rate of change of fluorescence intensity of the identified parenchyma over time; and classifying the detected microaneurysm as leaky where the rate of change is positive and not leaky where the rate of change is negative or zero; and outputting the classifications of the detected microaneurysms.

This invention discloses a multimodal facial blood perfusion image acquisition device and intelligent analysis system for combined infrared thermal imaging and fundus microcirculation imaging, comprising the following steps: S1, filling out a data acquisition form and collecting relevant data; S2, conducting a personalized breath-holding test; S3, acquiring infrared images of the user's face and acquiring color fundus images at different times after pupil dilation of the patient's left and right eyes; S4, converting the acquired color fundus images into fluorescein angiography (FFA) images using an improved Real-ESRGAN model; S5, extracting features from the facial infrared images and the generated FFA images respectively; S6, fusing the obtained infrared features and FFA features; S7, outputting the feature results. This invention acquires hemodynamic information from FFA non-invasively and combines it with facial infrared features for fusion analysis, enabling efficient and accurate acquisition and analysis of facial blood perfusion features, providing data support for related physiological research.