1028 results about "Fluorescein" patented technology

A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided.

The invention relates to fluorescence immune chromatography test paper which is formed by mutually and sequentially overlapping a sample pad, a combination pad, an antibody carrying film and water absorbing paper on a lining board with adhesive, wherein the combination pad is coated with a fluorescence material antibody 1 composite, a fluorescence-marked material is fluorescein granules or fluorescence material converted by rare earth, the antibody carrying film is a cellulose nitrate film or a nylon film and is respectively connected with an antibody 2 and the T line and the C line of a secondary antibody, the fluorescence material is fluorescein granules or the fluorescence granules converted by the rare earth, and the fluorescein granules are selected from isosulfocyanic acid fluorescein or tetramethyl isosulfocyanic acid rhodamine or tetraethyl rhodamine, and the surface of the fluorescence-marked material is aminated and combined with the antibody by cross-linking reaction. The fluorescence quantitative measuring system is used for detecting the fluorescence strength of the areas of the T line and the C line, and the quantitative detection is completed by the standard curve of the antigen concentration. The invention is suitable for the immune detection of tumor marked objects of urinal fiber-connected protein, and the like.

A handheld, ocular imaging device and system that employs the camera, processor and programming of a mobile phone, tablet or other smart device coupled to optical elements and illumination elements that can be used to image the structures of the eye in home-based, ambulatory-care, hospital-based, or emergency-care settings, is presented. The modular device provides multi-functionality (fluorescein imaging, fluorescence, brightfield, infrared (IR) imaging, near-infrared (NIR) imaging) and multi-region imaging (retinal, corneal, external, etc.) of the eye along with the added features of image processing, storage and wireless data transmission for remote storage and evaluation. Acquired ocular images can also be transmitted directly from the device to the electronic medical records of a patient without the need for an intermediate computer system.

The invention relates to an antibody chip kit for diagnosis of various tumors. The kit includes an antibody chip, a tumor marker standard substance mixture, a biotin-labeled tumor marker detection antibody mixture, and a fluorescein Cy3-labeled streptavidin, wherein the antibody chip includes a substrate, 16 kinds of tumor marker specific antibodies fixed on the surface of the substrate, and two kinds of positive controls, and the tumor marker standard substance mixture is a freeze-dried mixture obtained by mixing 16 kinds of standard tumor marker standard substances together according to a certain amount. The kit can detect 16 clinical commonly used tumor markers, overcomes the defects of complicated operation, single detection index, low sensitivity and the like in the prior art, has the advantages of being cheap, convenient, sensitive, accurate, high in throughput, and less in amount of samples, and can be popularized and large-scaled in common laboratories.

The invention belongs to the technical field of nano materials and biomedicine, in particular to multifunctional core-shell structure fluorescent coding magnetic microspheres and a preparation method thereof. On the basis of ferroferric oxide nanoparticles synthesized by a hydrothermal process, pre-prepared coupled product of fluoresceins and amino propyl trimethoxysilane and ethyl orthosilicate are subjected to cohydrolysis in ammonia water to form a multifunctional fluorescent magnetic nano composite material with fluorescent and magnetic properties and high biological stability and biological adaptability. By regulating the mixing ratio of fluoresceins, namely fluorescein isothiocyanate (FITC) and rhodamine B isothiocyanate (RBITC), various fluorescent coding magnetic microspheres can be prepared, and the particle size of obtained material can be regulated according to the different ratios of the added ethyl orthosilicate to the ferroferric oxide. In addition, the obtained multifunctional composite nano material is subjected to amino silanization modification, so the biological application range of the novel fluorescent coding magnetic microspheres is further expanded and the novel fluorescent coding magnetic microspheres have bright application prospect in fields of biomedicine technology, medicine development, suspension chip and the like.

The invention relates to a fluorescent ion probe (I) with high selectivity and high sensitivity in detection and the application of the fluorescent ion probe in identifying and detecting heavy metal ion and transition metal ion, wherein, the Y is an organic conjugate group with fluorescence transmitting function such as pyrene, naphthalene, 4-amidogen-1, 8-naphthyl imide, Dan sulfonamide, anthracene, carbazole, benzimidazoins, benzoxazoles, boron fluoride bipyrrole (BODIPY), fluorescein, 3, 4, 9, 10-perylenetetracarboxylic diimide or rhodamine B; the X is acylamino group, sulfoamino group or ester group. The fluorescent ion probe uses the fluorescence peak of fluorescence chromophore aggregate as the response signal to identify metal ion, thereby effectively avoiding the quenching effect of transition metal ion and heavy metal ion on fluorophore; moreover, the fluorescent ion probe realizes selective identification of heavy metal ion and transition metal ion in various solvents and aqueous solution in particular.

The invention provides a homogeneous luminescence immunoassay method for quantitatively analyzing multiple components simultaneously and a kit used for the method. Receptor microspheres containing various different fluoresceins are adopted, and antibody molecules for capturing different biological markers to be measured are enveloped in the method; in a measuring process, the multiple biological markers in a sample to be measured are combined with the corresponding antibody molecules on the surfaces of the receptor microspheres and biotinylated antibodies in a detection system respectively to form double-antibody sandwich compositions which are respectively connected with donor microspheres labeled with streptavidin; when the donor microspheres are irradiated by exciting light, all types of the receptor microspheres send out optical signals with different wavelengths; the intensities of the light with different wavelengths are respectively detected, so that the biological markers to be measured can be accurately quantified. The kit comprises the receptor microspheres containing chemiluminescence reagents and the fluoresceins, the biotinylated antibodies and the donor microspheres containing photosensitive substances. The homogeneous luminescence immunoassay method and the kit have the beneficial effects that simultaneous and quantitative measurement on multiple components is realized, and the detection cost is reduced.

The invention discloses a reversible terminal and synthesis and use in DNA synthesis sequencing thereof. The structural formula of the reversible terminal is described according to formula (I), wherein R1 is fluorescein and R2 is a connection unit. The cracking reversible terminal of the invention is available for DNA synthesis sequencing. At the same time, as synthesis required material is easily available and synthesis processes are all conventional chemical reactions, the reversible terminal disclosed by the invention is applicable for large-scale promotion and has good practical prospect due to a biological evaluation result showing that the reversible terminal is capable of totally satisfying biochemical reaction requirements of high-energy sequencing.

The invention discloses a four-color fluorescence labeling reversible terminal and a use thereof in DNA (Deoxyribonucleic Acid) sequencing. The structural formula of the reversible terminal is as shown in a formula (I) in the specification, wherein R1 is triphosphate; R2 is H or OH; the basic group is U, C, A, G or derivatives thereof; the connecting unit is a bifunctional compound which is breakable under a mild condition; the fluorescence group is one selected from a combination of BODIPY, fluorescein, rhodamine, coumarin, xanthene, cyanin, pyrene, phthalocyanine, alexa, squarene dye and an energy transferring dye, and derivatives thereof. The reversible terminal provided by the invention can be used for DNA single-molecule sequencing; simultaneously, raw materials required by the synthesis of the reversible terminal provided by the invention are simple and easy to get and the synthesis process of the reversible terminal is completely involved with conventional chemical reactions, so that the four-color fluorescence labeling reversible terminal can be popularized and utilized to a large scale; biological evaluation results indicate that the reversible terminal is capable of completely meeting the biochemical reaction requirements of high-flux sequencing and has a good practical prospect.

In lithography, a composition comprising a novolak resin comprising recurring units of fluorescein is used to form a photoresist underlayer film. The underlayer film is strippable in alkaline water, without causing damage to ion-implanted Si substrates or SiO₂ substrates.

The invention relates to a chemical illumination immunity analysis method for checking human cTnT, which uses acridiniumester and/or alkaline phosphatase as label. The immunity reaction uses two-site immunoassay and/or competition law. The immunity reaction can use streptavidin-biotin two-site immunoassay to improve sensitivity. The invention uses NaOH and H2O2 as acridiniumester illumination initiating agent, uses 1, 2-dioxo cyclohexane derivative (adamantine derivative) as the illumination substrate of alkaline phosphatase, and uses fluorescein derivative and surface activator as illumination renforcing agent. The solid carrier is orifice plate, macromolecule polymer tube (ball) and ferriferrous oxide magnetic particles or the like.

The invention provides a method for single-tube multiplex fluorescent polymerase chain reaction (PCR) detection of human coronavirus OC43, 229E, NL63, HKU1 and SARS. The method adopts primers having sequences of SEQ ID NO: 1-10, and probes having sequences of SEQ ID NO: 11-15. The invention also provides a kit for single-tube multiplex fluorescent PCR detection of human coronavirus OC43, 229E, NL63, HKU1 and SARS. The kit contains the primers and the probes. The method provided by the invention adopts the primers which are specific primers of OC43, 229E, NL63, HKU1 and SARS, and the probes which are Taqman probes, utilizes TAMRA/CY5/FAM/ROX/JOX multiple fluorescein labeling, realizes single-tube multiplex detection of human coronavirus OC43, 229E, NL63, HKU1 and SARS, and has the advantages of strong specificity, high sensitivity, fast detection speed, simple and convenient operation and low cost. The primers and the probes can be used as detection reagents for a scientific research and clinical application.

This invention relates to a method for dyeing polymer microspheres to obtain fluorescent polymer microspheres. The method comprises: uniformly mixing fluorescein 0.01-80%, polymer microspheres 0.1-80%, emulsifier 0-10%, tackifier 0-10%, and solvent (one or more of good solvents and poor solvents) 18.9-99.89%, dyeing under 1-101 kPa in dark for 1-720 h, taking out the dyed polymer microspheres, and washing repeatedly. The obtained fluorescent polymer microspheres have such advantages as simple process, high fluorescent brightness, wide fluorescent spectrum range, uniform particle size distribution and low relative standard deviation, and can be used as absolute counting microspheres of flow cytometry, fluoroimmunoassay microspheres, biosensor, microfluidic chip, and calibrator of fluorescence microscope.

The invention discloses a test strip for detecting aflatoxin B1 or M1 by utilizing an aptamer. The test strip for detecting the aflatoxin B1 or M1 by utilizing the aptamer, provided by the invention, comprises a sample absorption pad, a marker pad, a reaction film and a water absorption pad, wherein the marker pad is coated with a detection probe; the detection probe is an aflatoxin B1 aptamer marked by fluorescein; the nucleotide sequence of the aflatoxin B1 aptamer is sequence 1; the reaction film comprises a detection region and a quality control region; the detection region is coated with a conjugate formed by an aflatoxin B1 hapten and a carrier protein; and the quality control region is coated with a quality control probe, and the quality control probe is a conjugate formed by avidin conjugation probe marked aflatoxin B1 complementary single strand DNA (Deoxyribose Nucleic Acid)) molecules. The test strip for detecting the aflatoxin B1 or M1, provided by the invention, has the advantages of high sensitivity, accuracy in quantifying, strong specificity, simplicity and convenience, and short detection time.

The invention relates to a preparation method of magnetic fluorescence dual-function silicon oxide hollow microspheres, comprising the steps of: preparing magnetic nanometer particles by a coprecipitation method; diffusing the magnetic nanometer particles in long-chain alkane after the surfaces of the magnetic nanometer particles are modified by oleic acid; mixing the oil phase composed of alkanedispersion consisting of a styrene monomer, a superhydrophobic agent and magnetic nanometer particles, and the orthosilicic acid alkyl ester with water phase in which a surface active agent is dissolved; pre-emulsifying and finely emulsifying the mixture to obtain a fine emulsion drop system; when the drops are in free radical polymerization, adding an alkali catalyst to control the generation ofsilicon oxide and the phase separation of the organic and inorganic components of the system; in the reaction process, adding proper ammonia water and a silane coupling agent which is marked by fluorescein to obtain the hollow compound microspheres which are different in sizes, inorganic shell thicknesses and magnetic particle solid content and have stable fluorescence signals. The preparation method disclosed by the invention is simple, the raw materials are low in cost and easy to obtain; and the obtained fluorescence dual-functional hollow silicon oxide microspheres are narrow in size distribution, high in magnetic substance content and stable in fluorescence performance.

The invention provides a fluorescence probe, the synthetic method and the use which is to test the cell hydroxyl free radical. The process is: a. heat and chloroauric acid of 150.0 weight concentration and back flow, with mixing, the sodium citrate of 5.0-7.0 is added into the solution to back flow for 10-20min, then to cool in room temperature to get the golden nanometer particle solution; b. mix the particle with the DNA single chain with 5'end modified fluorescein and 3'end modified sulfydryl according to 1:200-500, then to set in room temperature for 1224h, last to adjust the pH by the phosphoric buffer; next to mix the mixture with the 0.5-5mol/L NaCl according to 1:0.1-0.3(V/V) which is set in room temperature for 35-45h; last to centrifugate to remove the upper solution and the deposition is solved in the 0.1-0.3mol/L phosphoric buffer which is the fluorescence probe solution.

The present invention is directed, in part, to fluorescein-based ligands for detection of metal ions, and methods of making and using the same.

The invention discloses a fluorochrome taking fluorescein as matrix, as well as a preparation method and application thereof. The fluorochrome taking the fluorescein as the matrix has the structure shown in a general formula I (figure 1); and the compound has a certain level of water-solubility, and has certain good membrane permeability. The compound disclosed by the invention has novel spectral characteristics; and dye of fluorescein derivative with good property is applied to the fluorescence imaging aspect.