24 results about "Immune complex" patented technology

This invention discloses a quantitative test strip for HBV PreS1 antibody based on the AIE molecule, its preparation method, and its applications, relating to the fields of molecular biology, immunology, and biodetection technology. The test strip includes a sample pad, a labeling pad, a nitrocellulose membrane (NC membrane), an absorbent pad, and a base plate. The NC membrane detection line is coated with HBV PreS1 antigen to capture PreS1 antibodies in the sample. The labeling pad contains an immobilized Anti-Human IgG antibody conjugated to the AIE molecule, which binds to the PreS1 antibodies in the sample to form an immune complex. The test strip of this invention enables rapid detection of PreS1 antibodies and achieves enhanced fluorescence in the detection results. Combined with a fluorescence reader or fluorescence card reader, the detection results can be digitally and quantitatively analyzed. It is simple to operate, rapid in detection, and significantly improves detection sensitivity and enables quantitative analysis.

The application discloses a kind of lipoprotein detection reagent and lipoprotein detection method, it is related to biochemical detection technical field, the lipoprotein detection reagent includes R1 reagent and R2 reagent, wherein, the R1 reagent includes anti apolipoprotein B antibody, the R2 reagent includes latex microsphere coated with lipoprotein specificity antibody. Wherein, anti apolipoprotein B antibody can preliminarily enrich target lipoprotein and form immune complex to amplify detection signal, and lipoprotein specificity antibody can specifically recognize target protein and form immune complex-lipoprotein specificity antibody-latex microsphere complex, further amplify detection signal, significantly improve the detection sensitivity of reagent.

This application discloses antibodies or antigen-binding fragments thereof that specifically bind to estradiol, immune complexes comprising the same, single-chain antibodies that specifically bind to the immune complexes, methods for their preparation, and uses. The antibody or antigen-binding fragment specifically binding to estradiol comprises a first heavy chain variable region and a first light chain variable region; wherein the first heavy chain variable region comprises first heavy chain CDR1, first heavy chain CDR2, and first heavy chain CDR3, and the first light chain variable region comprises first light chain CDR1, first light chain CDR2, and first light chain CDR3, and each of the six CDRs comprises an amino acid fragment with the sequence shown in SEQ ID NO: 1-6. Applying this antibody or antigen-binding fragment specifically binding to estradiol, along with ScFv selected based on it, to the sandwich method for detecting E2 in serum can significantly reduce background signal and improve the signal-to-noise ratio.

This invention discloses a chemiluminescent immunoassay method, comprising the following steps: labeling disulfide-bonded biotin or desulfobiotin onto affinity ligand one to form complex one; labeling a luminescent compound onto affinity ligand two to form complex two; mixing the sample with a solid-phase carrier containing complex one, complex two, and streptavidin-coupled compound to form an immunosandwich complex; processing the immunosandwich complex to release the immunosandwich complex from the solid-phase carrier; separating the immunosandwich complex from the solid-phase carrier; detecting the total luminescent signal value of the separated immunosandwich complex; and calculating the concentration of the analyte based on the total luminescent signal value. This invention releases the immunosandwich complex from the solid-phase carrier, uses a magnetic field to attract the solid-phase carrier, and measures the supernatant of the released immunosandwich complex. Solid-phase carriers containing non-specific adsorption cannot enter the measurement chamber, completely eliminating the problem of non-specific adsorption on the surface of magnetic beads.

The application belongs to the technical field of nanomaterials and biological analysis, and particularly relates to a ratio type photoelectrochemical analysis method based on multifunctional CeO2 / CdS and interface etching. First, a multifunctional CeO2 / CdS nanocomposite is synthesized by an in-situ growth method, and the nanocomposite has photoelectric properties of CdS and CeO2 peroxidase activity; second, a beacon antibody labeled with nanogold and glucose oxidase is prepared by an electrostatic adsorption method, a target protein, the beacon antibody and an antibody coated on an enzyme-labeled microplate are mixed and incubated to form an immune complex, and after glucose is added, H2O2 is generated by catalysis of the glucose oxidase on the beacon antibody; the generated H2O2 is catalyzed by CeO2 in the CeO2 / CdS nanocomposite to generate hydroxyl radicals, and in-situ etching of CdS in the electrode interface reduces the photocurrent, and a photocurrent intensity ratio (Iw / Ic) of a double-channel electrode has a linear relationship with the concentration of the target protein, so that quantitative analysis can be realized. The application has a sensitive response to the detection of proteins and has a fast detection speed.

The application discloses a platelet antibody detection and cross matching blocking chromatography kit and a detection method thereof, wherein the kit comprises a U-shaped microwell plate, a chromatography medium for separating platelet immune complexes from non-cell particle components under the action of centrifugal force, freeze-dried platelets, a platelet diluent, a platelet fluorescent staining solution comprising fluoresceinized platelet antibodies specifically labeled for platelets, biotin-labeled anti-human globulin for specifically binding with antibodies bound to the surface of the platelets, avidinized fluorescein for coupling with the biotin-labeled anti-human globulin through a biotin-avidin specific binding system, negative control serum / plasma and positive control serum / plasma. The application combines the blocking chromatography technology with a high-sensitivity flow immunofluorescence method to construct an efficient and accurate platelet antibody detection and cross matching system, which is high in specificity and sensitivity, simple in operation and stable in detection results.

The application discloses a carbon-coated zirconium oxide composite material, a colorimetric / photothermal dual-mode signal nano probe and application thereof in rapid quantitative detection of chikungunya virus. The colorimetric / photothermal dual-mode signal nano probe is formed by coupling a carbon-coated zirconium oxide composite nano material obtained by calcining mesoporous UiO-66 in an inert atmosphere with a chikungunya virus E2 protein monoclonal antibody. In a specific application, a chromatography test strip is constructed based on the colorimetric / photothermal dual-mode signal nano probe. The chromatography test strip comprises a nitrocellulose membrane provided with a T line and a C line. During detection, a sample and the nano probe form an immune complex and generate a colorimetric signal visible to the naked eye at the T line, and meanwhile, under the irradiation of 808 nm near-infrared laser, a temperature rise Delta T related to the concentration of a target object is generated at the T line, so that on-site rapid screening and photothermal quantitative review are realized. The chromatography test strip has both simple operation and reliable quantification, and has stronger anti-interference ability in a complex biological matrix, and is suitable for instant detection of chikungunya virus.

The application belongs to the technical field of genetic engineering antibodies, and particularly relates to an antibody for non-competitive detection of aflatoxin B1 and application thereof. The antibody comprises a nanobody of an aflatoxin B1 antigen-antibody immune complex and a monoclonal antibody against aflatoxin B1. The amino acid sequence of the nanobody of the aflatoxin B1 antigen-antibody immune complex is shown in SEQ ID NO. 1. The monoclonal antibody against aflatoxin B1 comprises a heavy chain and a light chain. The amino acid of the heavy chain is shown in SEQ ID NO. 10, and the amino acid of the light chain is shown in SEQ ID NO. 11. The antibody for non-competitive detection of aflatoxin B1 has low cost, can be prepared in a large amount through a prokaryotic expression system, is simple to prepare, has strong operability, and can be applied to non-competitive immunological analysis of aflatoxin B1.

This invention provides an antitumor agent that demonstrates efficacy against cancer cells expressing CD123, which has been reported to be overexpressed in malignant cells of a wide range of hematological malignancies. [Solution] Provided are an antibody or its antigen-binding fragment, an immune complex containing the antibody, and a method for using such CD123-binding molecules to diagnose and treat diseases such as B-cell malignancies, (a) an epitope within amino acids 101-346 of the human CD123 / IL3-Rα antigen, and (b) an antibody or its antigen-binding fragment that inhibits IL3-dependent proliferation of antigen-positive TF-1 cells.

The application provides a biomarker combination for non-invasive diagnosis of IgA nephropathy, comprising IgA-IgG immune complexes, IgG-IgA1 immune complexes, and IgA immune complexes combined with CD89. The application also provides an enzyme-linked immunoassay kit for auxiliary diagnosis of IgA nephropathy, which can jointly detect the levels of IgA-IgG immune complexes, IgG-IgA1 immune complexes, and IgA immune complexes combined with CD89. The application further provides an evaluation method for case damage of kidney tissue, which is based on the joint detection of the levels of IgA-IgG immune complexes, IgG-IgA1 immune complexes, and IgA immune complexes combined with CD89 in a sample, calculates a multi-index prediction score, and sets an evaluation threshold to determine whether short glomeruli have pathological damage. When the biomarkers are used for auxiliary diagnosis of IgA nephropathy, the biomarkers not only have the characteristics of non-invasiveness, but also have higher sensitivity, higher specificity and higher stability, and have clinical application value.

This invention discloses Astragalus polysaccharide, extracted from Astragalus membranaceus. The average molecular weight of this Astragalus polysaccharide is 8-12 kDa, and its monosaccharides are mainly composed of galacturonic acid, glucose, galactose, and arabinose. This Astragalus polysaccharide is a GalA-type acidic polysaccharide that effectively reduces the deposition of immune complexes and the release of pro-inflammatory factors in renal tissue by regulating signaling pathways such as cGAS-STING. Simultaneously, it improves glomerular filtration function by stabilizing the podocyte cytoskeleton structure, exhibiting a synergistic protective therapeutic advantage. This invention also discloses a method for preparing this Astragalus polysaccharide.

Disclosed in the present invention is the use of an anti-Gelsolin3 antibody related to demyelinating Guillain-Barré syndrome. The use mainly comprises the following steps: collecting the serum from a GBS patient and a control individual, performing co-immunoprecipitation with a sciatic nerve protein to obtain an immune complex, separating and identifying the immune complex by using a mass spectrometer to obtain a candidate protein sequence, inserting a candidate protein coding gene into a vector, transfecting cells followed by immunofluorescence verification to determine the localization and expression of the candidate protein, constructing a candidate protein expression plasmid, expressing and purifying a recombinant protein in Escherichia coli, performing co-immunoprecipitation verification on the purified recombinant protein by using the serum from the GBS patient to determine the presence of an anti-Gelsolin3 IgG antibody, further verifying the expression of the anti-Gelsolin3 IgG antibody in the serum from the GBS patient by using a Western blot method, and verifying the binding of the serum from an anti-Gelsolin3 IgG antibody-positive patient to the sciatic nerve by using a single-fiber immunofluorescence method.

The present application belongs to the field of medicine and detection, and relates to an antibody and a kit for anti-estradiol immune complex. The antibody of the present application can specifically bind to the estradiol immune complex, but cannot bind to the estradiol alone, nor can bind to the estradiol-specific antibody alone. The kit prepared by the antibody of the present application can effectively determine the estradiol, and has extremely high sensitivity and specificity.

The application relates to a separation method of cholesterol in an immune complex and a detection method of a cholesterol empty package rate, and belongs to the technical field of vaccines. The separation method of cholesterol in the immune complex comprises the following steps: (1) adding an excess of positively charged substances to a solution containing the immune complex for mixed adsorption, and then adding an excess of negatively charged substances to the solution for mixed adsorption; wherein the immune complex comprises quillaja saponins and cholesterol, and the quillaja saponins are adsorbed on the cholesterol; (2) separating the precipitate after the mixed adsorption, and the cholesterol in the supernatant is cholesterol that is not adsorbed with the quillaja saponins. The cholesterol adsorbed with the quillaja saponins and the cholesterol not adsorbed with the quillaja saponins can be completely separated, so that the specific distribution of the quillaja saponins on the cholesterol in the liposome can be more accurately detected.

This invention discloses a competitive homogeneous immunoassay method based on upconversion nanoparticles. The method involves conjugating activated upconversion nanoparticles to an antigen; enriching and purifying the upconversion nanoparticle-antigen probe; mixing the donor with specific antibodies and free antigens at different concentration gradients, followed by an immunoassay; receptor labeling; and detecting the fluorescence intensity of the reaction solution. The concentration of the antigen in the sample to be tested is obtained from the curve of the change in free antigen concentration versus fluorescence intensity, or the concentration of the target analyte is obtained at the single-particle level by counting the quantitative changes in the immune complex. This invention effectively solves the problems of high background noise and low signal-to-background ratio in resonance energy transfer (RET). By labeling the formed semi-sandwich structure immune complexes using a receptor deposition method, receptors accumulate around the immune complexes, increasing the number of receptors and shortening the distance between the donor and receptor, significantly improving RET efficiency and signal strength, thereby enhancing the sensitivity of the competitive homogeneous immunoassay.

This invention discloses a non-competitive lateral flow immunoassay method based on dual-signal mode of AIE nanoparticles, belonging to the field of agricultural product detection technology. This invention uses an organic solvent swelling method to prepare PSNPs@AIE. 650 Hydrophilic AIE nanoparticles were used to label anti-immune complex peptides (AICPs) with streptavidin-biotin system via an active esterification method to obtain PSNPs@AIE. 650 @AICP immunorecognition probe; Construct a dual-signal non-competitive lateral flow immunochromatographic system for the detection of thiamethoxam residues in agricultural products. This invention achieves colorimetric + fluorescence dual-signal readout, with a colorimetric visualization detection limit of 1.0 ng / mL and a quantitative detection limit of 0.45 ng / mL; a fluorescence visualization detection limit of 0.25 ng / mL and a quantitative detection limit of 0.23 ng / mL. It has the advantages of high sensitivity, strong specificity, rapid portability, and resistance to matrix interference, effectively solving the problem of insufficient sensitivity in traditional colloidal gold lateral flow immunoassay, and is suitable for rapid on-site detection of small molecule pesticide residues in agricultural products.

This invention discloses a PSA detection method based on a black phosphorus / bismuth vanadate composite material, comprising: S1, coating a microplate with a PSA monoclonal antibody, blocking it, adding the PSA sample to be tested and incubating it, then incubating it with a PSA polyclonal antibody, a thiolized oligonucleotide sequence 1, and a gold nanoparticle conjugate to form a sandwich immune complex; S2, adding a circular DNA template, DNA ligase, and other reagents, and incubating to complete RCA amplification; S3, adding a thiolized oligonucleotide sequence 2 and a conjugate with alkaline phosphatase, and adding a substrate for hydrolysis to generate ascorbic acid; S4, using a biosensor to perform photoelectrochemical testing under simulated sunlight and voltage, recording the oxidation current signal to quantify the PSA concentration. The PSA detection method disclosed in this invention, using a biosensor modified with this composite material and employing a signal amplification strategy, achieves highly sensitive and stable detection of PSA, which has significant clinical implications for the early diagnosis of prostate cancer.

The application discloses a kind of NiFe-LDH synergistic electrocatalysis enhanced Zr-MOF coordination aggregation induced electrochemiluminescence immunosensor and its preparation method and application, belong to electrochemiluminescence biosensor technical field.The sensor electrode is used as substrate, first NiFe-LDH catalytic layer is constructed on the surface of electrode, then Zr-MOF / BSA / Ab1 recognition layer is modified, then H-FABP is introduced by antigen antibody specific recognition, finally ZIF-8@CCM@AuNPs / BSA / Ab2 quenching probe is added to form sandwich type immune complex structure, and the electrochemiluminescence detection of H-FABP is realized in K2S2O8 co-reaction system.NiFe-LDH can synergistically catalyze activation of persulfate, enhance the electrochemiluminescence signal of Zr-MOF;Zr-MOF is limited by coordination effect Molecular movement improves the stability and luminous efficiency of luminescence.The immunosensor constructed by the application has the advantages of high sensitivity, wide linear range, good repeatability, strong stability and good selectivity, and can be used for quantitative detection of H-FABP in serum sample.

This invention belongs to the technical field of in vitro detection and discloses a long-persistent homogeneous detection method. Through an immune reaction or binding in a homogeneous solution, a long-persistent immune complex is formed and immobilized on the bottom plate of the test container. First, interference or influence caused by non-immune binding is effectively removed by injecting a separation solution. Based on this, long-persistent luminescent surface imaging signal acquisition technology is used to achieve high-quality detection without background, and to simultaneously and conveniently perform rapid detection on multiple samples. The long-persistent homogeneous detection method described in this paper provides a new approach for high signal-to-noise ratio and high-throughput long-persistent homogeneous detection.

The idea of the patent includes a new principle, which is the detection of immune complexes using ELISA technology, which is a new and modern principle that is added to the four principles of ELISA, the new principle (the fifth ELISA) depends on detection of immune complexes, which consist of two different parts of proteins (an antigen - an antigen). This complex can be detected through this new principle, and it was applied to the immune complex (thyroid-stimulating hormone receptors and antibodies formed against it) Hashimoto's patients. It was considered an example of applying this theory, the immune complex was actually detected, its concentration determined, and compared to other techniques. A standard solution specially designed for this purpose was used to prove this theory. In this patent, a new kit is specifically designed to detect these immune complexes. Immune complex, the group is considered successful. In terms of use.

The application discloses a traditional Chinese medicine composition for reducing urinary protein and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine. The traditional Chinese medicine composition is composed of radix astragali, caulis sinomenii, caulis sargentodoxae, dioscorea nipponica, abelmoschus manihot flower, gordon euryale, and ligusticum sinense in specific proportions. Multiple components are synergized. Through the synergistic anti-inflammatory effect of the double-cane-one-dragon wind-removing structure, the kidney collateral penetration is enhanced, and the antibody clearance rate is improved. The radix astragali and gordon euryale are synergistically used to protect the podocytes. The three-dimensional synergy of "dispelling wind and dredging collaterals, consolidating essence and qi, and removing dampness and detoxifying" is formed. The closed loop of "reducing leakage, enhancing resistance, and promoting repair" is formed. Clinical and animal experiments show that the 24-hour urinary protein reduction rate is more than 60%, the effective rate is 80%, the deposition of immune complexes can be reduced synchronously, the podocytes can be repaired, and the function of the renal tubules can be improved. The traditional Chinese medicine composition provides an efficient and safe solution for the increase of urinary protein in multiple types of kidney diseases.

This invention relates to a droplet microfluidic-based PSA detection method, which solves the technical problems of high detection limit and low sensitivity in existing ELISA methods for detecting the prostate cancer biomarker PSA. The method first prepares an oil phase, then mixes an immune complex suspension and FDG substrate to prepare an aqueous phase, then generates and captures droplets in a flow-focusing microfluidic chip to form a monolayer droplet array, and finally incubates and performs fluorescence immunoassay to achieve quantitative detection of PSA.

This invention relates to the field of biochemical analysis technology, specifically providing a rapid quantitative point-of-care detection method and system based on artificial intelligence signal prediction. The method includes: homogeneously reacting non-magnetic microspheres and magnetic nanoparticles, with surfaces modified with different biorecognition molecules, with a test solution to form an immune complex; injecting this complex into a self-driven microfluidic chip, performing magnetophoretic separation in a magnetic field, and anchoring the complex to a linear microtube array; acquiring chip images within 20 seconds of signal transduction initiation; determining the weight of each microtube through physical field simulation, and calculating the early measured total pixel intensity by combining machine vision pixel intensity recognition; finally, inputting this intensity into a trained AI prediction model to output the predicted endpoint signal value, and combining it with a calibration curve to quantify the analyte. This method reduces detection time from hours to minutes, combining high precision, portability, and versatility, and is suitable for rapid on-site detection of pathogens, biomarkers, and small molecule pollutants.

The application discloses an AIE molecule-based HBV PreS1 antibody quantitative detection test strip as well as a preparation method and application thereof, and relates to the fields of molecular biology, immunology and biological detection technology.The test strip comprises a sample pad, a mark pad, a nitrocellulose membrane (NC membrane), a water absorption pad and a bottom plate, the NC membrane detection line is coated with an HBV PreS1 antigen and is used for capturing PreS1 antibodies in a sample, and the mark pad is fixed with Anti-Human IgG antibodies coupled with AIE molecules and is used for combining with the PreS1 antibodies in the sample to form an immune complex.The test strip can realize rapid detection of the PreS1 antibodies, realize a fluorescence enhancement effect of a detection result, and, in combination with a fluorescence reading instrument or a fluorescence reading card equipment, the detection result can be subjected to digital quantitative analysis, is simple and convenient to operate, rapid to detect, significantly improves detection sensitivity and realizes quantitative analysis.