153 results about "Chemiluminescent immunoassay" patented technology

The invention relates to a chemiluminescent immunoassay analyzer. The analyzer comprises a reagent dosing device, a sample delivering device, an automatic cup neatening device, an emergency treatment device, a reaction disk device, a cleaning device, a sample diluting device, a luminescence measuring device, a collecting barrel, a manipulator and a sampling needle, and the sampling needle is used for sucking and conveying a liquid, and can directly swing among the sample delivering device, the emergency treatment device and the sample diluting device; the manipulator can grasp and convey reaction cups to make the reaction cups move to the reaction disk device from the automatic cup neatening device, move to the cleaning device from the reaction disc device, move to the luminescence measuring device from the cleaning device and move to the collecting barrel from the luminescence measuring device; and the reaction cups and the liquid are conveyed through the movement of the manipulator and the sampling needle among all above devices in order to realize the automatic operation of the whole immunoassay process and improve the full automation degree.

The invention discloses a dual-enhanced chemiluminescent immunoassay method based on metal enhanced luminescence and nano particle labelled amplification. According to the dual-enhanced chemiluminescent immunoassay method based on metal enhanced luminescence and nano particle labelled amplification, metal nano particles replace conventional polystyrene nano microspheres, and luminescence substances are lebelled on the surface of an isolation layer spaced from the surfaces of the metal nano particles by 5-20nm; chemiluminescence of luminescence substances on sensitized metal nano particles is coupled to plasma waves on the surfaces of the metal nano particles; after resonance is generated, the chemiluminescence is emitted in the form of relatively high light intensity and relatively high attenuation speed. On the basis of a conventional chemiluminescence immunoassay technology, a metal enhanced luminescence technology and a nano particle labelled amplification technology are organically integrated; therefore, the dual-enhanced chemiluminescent immunoassay method has the advantages of high sensitivity, high detection speed and the like.

The invention relates to the medical field of immunoassay, more specially, the invention provides a chemiluminescent immunoassay detection kit for des-gamma-carboxyand-prothrombin (DCP) microporosity plate and a preparation method thereof, and realizes the rapid, sensitive and high-specificity serological detection of DCP with the one step chemiluminescent immunoassay method. The kit of the invention has the advantages of simplicity, convenience, rapidness, sensitivity, stability and the like, eliminates the interference of the prothrombin analogues and the serum cellulose and the analogues thereof, and has the advantage of high specificity.

The invention discloses a toxoplasma gondii IgM antibody detection kit combined with the FITC-anti-FITC indirect coating technology and the chemiluminescent immunoassay technology, and a preparation method thereof. The kit of the invention is composed of a negative control, a positive control, solid-phase vectors for anti-FITC antibodies, anti-human Mu-chain monoclonal antibodies of FITC markers, toxoplasma gondii antigens which are marked by horse radish peroxidase, chemiluminescent substrates and concentrated washing solutions. The kit of the invention can be used as the aided detection index for prenatal prepotency diagnosis, and has vital significances for improving the birth population quality and doing the family planning and the prepotency well.

The invention relates to the medical field of immunoassay, more specially, the invention provides a chemiluminescent immunoassay detection kit for phosphatidylinositol proteoglycan-3(GPC-3) and a preparation method thereof, and realizes the simultaneous serological detection of GPC-3 N terminal and C-terminal protein with the chemiluminescent immunoassay method. The kit has the advantages of simple sampling, convenient detection and accurate and specific technical method. The invention adopts a biotin-strapavidin system to coat antibodies and improve the efficiency of antibody coating and the linear range of detection as well as sensitivity, and can be conveniently used for the tracing observation of early diagnosis or treatment effect for primary carcinoma of liver.

The invention discloses a light induced chemiluminescent immunoassay reagent kit for aflatoxin B1and a detection method thereof, which belong to the technical field of light induced chemiluminescent immunoassay. A luminescent particle enveloped with AFB1-BSA is added into a microporosity plate, a AFB1 standard or sample, a rabbit anti-AFB1 antibody, a biotin goat anti-rabbit antibody are sequentially added for a photophobic reaction, then a photosensitive particle enveloped with streptavidin is photophobically added, and the mixture is incubated and then is detected. The AFB1-BSA enveloped on the luminescent particle competes with the AFB1 in the standard or sample for connecting to the AFB1 antibody to form a complex with the biotin goat anti-rabbit antibody and the photosensitive particle enveloped with the streptavidin, the energy is transferred to the luminescent particle to produce fluorescence by producing and transferring singlet ionic oxygen under optical excitation, a light induced chemiluminescent detector is used to detect, the intensity of optical signals is inversely proportional to the concentration of the AFB1, and the content of the AFB1 in the measured sample is determined by contrasting with the standard curve. The invention is used to detect the content of the AFB1 in foodstuff, feedstuff and products of the foodstuff and the feedstuff; and the reagent kit has the advantages of simple structure, simple and convenient operation, low cost, short detection time, and high sensitivity.

The invention relates to a sST2 detection kit based on microparticle chemiluminescence immunoassay, relating to a chemiluminescence detection kit. The sST2 detection kit is equipped with reaction buffer, magnetic microparticles coated with sST2 monoclonal antibody, sST2 monoclonal antibody labeled with acridinium ester, luminescent liquid, sST2 antigen standard solution, and concentrated washing liquid. Prepare reaction buffer; prepare magnetic particles coated with sST2 antibody; prepare acridinium ester-labeled sST2 antibody; prepare sST2 antigen standard solution; prepare luminescent liquid; prepare washing liquid. Using microparticle chemiluminescence immunoassay technology, it has higher sensitivity and better stability than ELISA, and fills the gap in the production of microparticle chemiluminescence diagnostic reagents for human serum sST2 detection in China. It has the advantages of simple operation, high sensitivity, wide linear range, stable results, good safety, and easy automation, and has broad application prospects in clinical testing and other aspects.

The invention discloses a chemiluminescent detection kit for interleukin-6 and a preparation method thereof. The kit includes: sample diluent, magnetic particles coated with interleukin-6 monoclonal antibody, interleukin-6 monoclonal antibody labeled with acridinium ester, interleukin-6 series standard solution, chemiluminescence excitation solution A, chemiluminescence excitation solution B. Cleaning solution. The kit of the invention combines the chemiluminescent technology with the immunomagnetic particle, and provides a nearly homogeneous reaction system. Compared with the prior art, the kit of the invention has the advantages of high sensitivity, strong specificity, short reaction time and the like.

The invention relates to the chemiluminescent immunoassay, in particular to a quantitative kit for detecting dog IL-6 by adopting homogeneous chemiluminescent immunoassay and a use method thereof. A detection solution adopted by the kit contains DNA1-IL-6 antibody1 conjugate, DNA2-IL-6 antibody2 conjugate, DNA3 for marking acridinium ester (AE), restriction enzyme and graphene oxide; DNA1 and DNA2have six complementary bases, the DNA3 contains two enzyme digestion sites, and has nine bases to complementarily pair with the DNA1 and the DNA2; the DNA3 is adsorbed on the surface of the grapheneoxide through the Pai-Pai stacking effect, and the chemiluminiscence of the AE marked at the tail end is quenched due to the occurring of the CRET. The method is the homogeneous immunoassay method, the operation is simple, and the analysis time is greatly shortened, the measure of the single sample can be accomplished in 5-10min, by combining the ortho-bunting effect and a chemiluminescent molecular beacon, a switch of the graphene quenching mechanism is induced through immunoreaction, thereby releasing a chemiluminiscence signal without washing, separation and purification steps.

The invention provides a thyrotropin (TSH) chemiluminescent immunoassay quantitative detection kit and a preparation method thereof. The invention has the advantages that the reaction pattern of the double antibody sandwich method is adopted, the chemiluminescent technology combining with the technology of fluorescin isothiocyanate-anti-fluorescein isothiocyanate is effectively utilized, horse radish peroxidase is adopted to be as the marker enzyme to quantitatively detect the content of TSH in the human serum sample, the detection sensitivity is guaranteed, and the raw materials are greatly saved. The kit of the invention has high sensitivity, good repeatability, short reaction time, good stability and long reagent validity, and can provide the experimental basis timely for the clinical diagnosis and the treatment of thyroid disorders.

The invention provides a chemiluminescent immunoassay kit for thyroid peroxidase autoantibodies and a preparation method thereof. The invention has the advantages that the reaction pattern of the double antigen sandwich method is adopted, the chemiluminescent technology and the biotin-avidin immune amplifying technological principle are effectively utilized, horse radish peroxidase is adopted to be as the marker enzyme, the content of the thyroid peroxidase autoantibodies in human serum samples is quantitatively detected, and the detection sensitivity can be ensured. The kit of the invention has the advantages of high sensitivity, good repeatability, good stability and high detection efficiency, and can provide a timely and reliable experimental basis for the clinical diagnosis and the treatment of thyroid disorders.

The invention discloses a chemiluminescent detection kit for procalcitonin and a preparation method thereof. The kit includes: sample diluent, magnetic particles coated with procalcitonin monoclonal antibody, procalcitonin monoclonal antibody labeled with acridinium ester, procalcitonin series standard solution, chemiluminescence excitation solution A, Chemiluminescence excitation solution B, cleaning solution. The kit of the invention combines the chemiluminescent technology with the immunomagnetic particle, and provides a nearly homogeneous reaction system. Compared with the prior art, the kit of the invention has the advantages of high sensitivity, strong specificity, short reaction time and the like.

The invention discloses an unmarked chemiluminescent immunosensor and an immunoassay method thereof. The immunoassay method comprises the following steps: (1) after fixing the immunosensor on an immune microreactor, injecting antigen samples into a flowing cell at the speed of 0.5 milliliter per minute, and carrying out on-line incubation to generate immune complexes; (2) flushing the immune complexes by virtue of a buffer solution PBST at the speed of 1 milliliter per minute, so as to remove unreacted immune reagents; and (3) introducing a chemiluminescent substrate solution into the immunosensor at the speed of 0.5 milliliter per minute, and recording generated chemiluminescent signals by virtue of a photomultiplier. The immunosensor is produced by jointly fixing a chemiluminescent probe and unmarked antibodies at a high-biocompatibility solid phase interface and is combined with flow injection, so as to construct an unmarked chemiluminescent immunoassay method having the advantages of low cost, rapidness and convenience.

The invention provides a chemiluminescent immunoassay kit for detecting clenbuterol, and the kit belongs to the field of immunoassay. The kit is composed of a non-transparent white enzyme-labeled plate which is coated by a clenbuterol-carrier protein cross-linked complex, a clenbuterol standard product, a clenbuterol-peroxidase-labeled antibody, luminescent substrate solution and concentrated buffer solution. The clenbuterol-carrier protein cross-linked complex is obtained by coupling the clenbuterol and a carrier protein through the mixed-anhydride method or the direct protein activation method, and the concentrated buffer solution contains Tween-20 buffer solution. The kit has the advantages of rapidness, simpleness, high sensitivity, low detection cost, good repeatability, high throughput, and the like, and the kit can be used in the detection of residual clenbuterol in animal urine, blood, tissues, visceral organs and other samples.

The invention belongs to the immunoassay technical field and relates to a hepatitis B e antibody magnetic particle chemiluminescent immunoassay kit. The kit of the invention comprises a mixed liquor of hepatitis B e antibody coated with magnetic particle and horse radish peroxidase-labeled hepatitis B e antibody, hepatitis B e antigen, hepatitis B e antibody calibrator, chemiluminescence substrate solution, concentrated cleaning solution and reaction tubes. The invention can be used to test the content of hepatitis B e antibody in human serum and has the advantages of simple and fast operation, good repetitiveness and the like.

The invention discloses a method for detecting diethylstilbestrol and a special chemiluminescent immunoassay kit thereof. The chemiluminescent immunoassay kit for detecting diethylstilbestrol comprises a diethylstilbestrol specific antibody, a coating antigen and a standard solution, wherein the coating antigen is a conjugate of diethylstilbestrol hapten and carrier protein, and the constitutional formula of the coating antigen is as shown in a formula I. The detection method has the characteristics of simple preprocessing of samples, easy operation, low cost, high specificity, high sensitivity, high accuracy, and the like, and is capable for on-site monitoring and suitable for screening a large amount of samples.

The invention provides a chemiluminescent immunoassay kit for detecting ractopamine, and the kit belongs to the field of immunoassay. The kit is composed of a non-transparent white enzyme-labeled plate which is coated by a ractopamine-carrier protein cross-linked complex, a ractopamine standard product, a ractopamine-peroxidase-labeled antibody, luminescent substrate solution and concentrated buffer solution. The ractopamine-carrier protein cross-linked complex is obtained by coupling the ractopamine and a carrier protein through the mixed-anhydride method or the direct protein activation method, and the concentrated buffer solution contains Tween-20 buffer solution. The kit has the advantages of rapidness, simpleness, high sensitivity, low detection cost, good repeatability, high throughput and the like, and the kit can be used for detecting the residual ractopamine in animal urine, blood, tissues, visceral organs and other samples.

The present invention relates to assays and kits for detecting or quantifying iron metalloprotein in test samples.