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Quantitative kit for detecting dog IL-6 by adopting homogeneous chemiluminescent immunoassay and use method thereof

A homogeneous chemiluminescence and IL-6 technology, which is applied in the field of quantitative kits for the detection of canine IL-6 by homogeneous chemiluminescence, can solve the problems of high equipment requirements, special labeling materials, and difficulty in obtaining, etc., and achieve simple and low cost , high specificity, strong versatility

Inactive Publication Date: 2018-12-18
南京浦光生物科技有限公司
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Problems solved by technology

This method requires high equipment, and the marking material is special and difficult to obtain

Method used

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  • Quantitative kit for detecting dog IL-6 by adopting homogeneous chemiluminescent immunoassay and use method thereof
  • Quantitative kit for detecting dog IL-6 by adopting homogeneous chemiluminescent immunoassay and use method thereof

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Embodiment Construction

[0030] The present invention is described in further detail now in conjunction with accompanying drawing. These drawings are all simplified schematic diagrams, which only illustrate the basic structure of the present invention in a schematic manner, so they only show the configurations related to the present invention.

[0031] Such as figure 1 As shown, the principle of the present invention lies in that the detection solution of the method includes DNA1-IL-6 antibody 1 conjugate, DNA2-IL-6 antibody 2 conjugate, DNA3 labeled with acridinium ester (AE), restriction internal Dicer (Nt.BbvCI) and graphene oxide (GO). The designed DNA3 contains 2 restriction sites, and 9 bases are complementary to DNA1 and DNA2 respectively. When no target protein exists, DNA3 is adsorbed on the surface of GO through π-π stacking, and the chemiluminescence of its end-labeled AE is quenched due to CRET, showing "signal off". In the presence of the target protein, antibodies on both Ab-DNA comp...

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Abstract

The invention relates to the chemiluminescent immunoassay, in particular to a quantitative kit for detecting dog IL-6 by adopting homogeneous chemiluminescent immunoassay and a use method thereof. A detection solution adopted by the kit contains DNA1-IL-6 antibody1 conjugate, DNA2-IL-6 antibody2 conjugate, DNA3 for marking acridinium ester (AE), restriction enzyme and graphene oxide; DNA1 and DNA2have six complementary bases, the DNA3 contains two enzyme digestion sites, and has nine bases to complementarily pair with the DNA1 and the DNA2; the DNA3 is adsorbed on the surface of the grapheneoxide through the Pai-Pai stacking effect, and the chemiluminiscence of the AE marked at the tail end is quenched due to the occurring of the CRET. The method is the homogeneous immunoassay method, the operation is simple, and the analysis time is greatly shortened, the measure of the single sample can be accomplished in 5-10min, by combining the ortho-bunting effect and a chemiluminescent molecular beacon, a switch of the graphene quenching mechanism is induced through immunoreaction, thereby releasing a chemiluminiscence signal without washing, separation and purification steps.

Description

technical field [0001] The invention relates to chemiluminescence immunoassay, in particular to a quantitative kit for detecting canine IL-6 by a homogeneous chemiluminescence method and a use method thereof. Background technique [0002] Chemiluminescence immunoassay (CLIA) is a combination of high-sensitivity chemiluminescence assay technology and high-specificity immune response for various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins and Drug detection and analysis technology. According to whether there is a separation and cleaning step, it can be divided into heterogeneous chemiluminescence method and homogeneous chemiluminescence method. At present, in the field of in vitro diagnostic testing, domestic and foreign testing products basically use heterogeneous chemiluminescence. Foreign manufacturers include Roche, Abbott, Beckman, Siemens, Sorin, and Hismicom, and domestic manufacturers include New Industry, Antu, Mike, Mindray, Zecheng, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/53G01N21/76
CPCG01N21/76G01N33/53G01N33/6854
Inventor 曹丹
Owner 南京浦光生物科技有限公司
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