443results about How to "High detection sensitivity" patented technology

The invention relates to an Internet of Vehicles multi-agent cooperative sensing method and system. The method comprises the following steps: constructing a collaborative sensing network, wherein the collaborative sensing network comprises a self-agent and a plurality of collaborative agents; acquiring and processing sensing data through the collaborative sensing network; performing feature extraction to obtain intermediate features; self-adaptive sparsification is carried out to obtain sparse features, and the sparse features are compressed and transmitted to a self-agent; performing time sequence feature enhancement on the features of all the agents at the self-agent end; fusing the features to obtain fused features; and constructing an Internet of Vehicles perception model, and realizing perception by the detection model according to the fused features. According to the method, the calculation complexity of traditional global attention is reduced from the square level to the linear level through an adaptive sparsification mechanism, the calculation overhead is remarkably reduced while the multi-agent feature interaction precision is kept, and the method is more suitable for real-time operation on the vehicle-mounted edge equipment with limited resources.

The invention belongs to the technical field of optical signal collection and imaging, and particularly relates to a multi-path nonlinear optical signal collection device. The adjusting table is used for bearing a to-be-detected sample and adjusting the spatial pose of the to-be-detected sample, the laser emitting module is used for emitting laser, the to-be-detected sample is excited by the laser to obtain a nonlinear optical signal, the nonlinear optical signal is incident to the overturning mirror through the reflection transmission unit or the transmission transmission unit, and the overturning mirror is used for adjusting the spatial pose of the to-be-detected sample through angle adjustment. The nonlinear optical signal is reflected to a first signal processing module or a second signal processing module, and the first signal processing module processes the nonlinear optical signal from the reflection transmission unit or the transmission transmission unit to obtain a spectral analysis processing result; and the second signal processing module processes the nonlinear optical signal from the reflection transmission unit or the transmission transmission unit to obtain an electric signal processing result. The device is used for collecting non-linear optical signals of far-end detection optical paths of reflection, transmission, oblique incidence and the like.

The invention discloses an ion trap mass spectrometry device and method capable of switching internal and external ion sources, and the device comprises an ion source cavity, the exterior of the ion source cavity is provided with a heating module, the side wall of the ion source cavity is provided with a sample introduction hole, and the sample introduction hole is internally provided with a chromatographic column I; the electron beam generation system is arranged at the front side position of the ion source cavity; the insulation connecting piece is communicated with the ion source cavity, and a lens I, a lens II and a lens III are sequentially arranged in the insulation connecting piece in the sample injection direction; a signal acquisition system; wherein a chromatographic column II is arranged on the ion trap. The two ionization modes inside and outside the trap are integrated, and different detection requirements can be flexibly met by switching the sample introduction path of the chromatographic column and regulating and controlling the voltage parameters of the lens I, the lens II and the lens III.

The invention belongs to the technical field of talc, and provides a method for detecting fluorescent substances in talc raw ore, which comprises the following steps: S1, carrying out ball milling on the talc raw ore in a ball mill, sieving to obtain sieved powder, and adding deionized water into the sieved powder to obtain slurry; s2, adding the slurry into the mixture to enable the pH value of the slurry to be 5, stirring, and standing in a water bath for 2-3 hours to obtain a first precipitate; s3, adding a thiourea-ascorbic acid solution into the first precipitate to obtain a second precipitate; and S4, carrying out secondary ball milling on the second precipitate, and spreading the second precipitate in an enamel plate to detect whether the talc raw ore contains the fluorescent substances or not. The hydrochloric acid can dissolve quartz and mica to avoid generation of background fluorescence or quenching effect, and the nonionic surfactant polyethylene glycol can be adsorbed on the surface of the mineral to reduce interfacial tension, so that the hydrochloric acid is easier to infiltrate tiny pores, the pit defect caused by local over-corrosion is reduced, the pickling uniformity is improved, and the detection sensitivity is improved.

This invention relates to the field of spectroscopic measurement technology, and particularly to a measurement system and method for measuring the concentration of high-temperature combustion gases in aero-engines. The measurement system includes a supercontinuum laser source, a first collimating mirror, a second collimating mirror, a spectrometer, and a computer. The measurement method includes acquiring the actual transmittance spectrum of the high-temperature combustion gases; calculating the theoretical transmittance spectrum of the high-temperature combustion gases; constructing constraints; fitting the actual transmittance spectrum and the theoretical transmittance spectrum into the constraints; and calculating the concentration of the high-temperature combustion gases. The measurement system has a lightweight structure, requiring only holes for mounting the laser emission and absorption devices on the inner wall of the aero-engine casing. The measurement method uses a supercontinuum laser source, which, compared to ordinary lasers used in active gas detection, offers better spatial coherence, a wider spectral adjustment range, and higher intensity, enabling the detection of more types of gases. The method uses a multi-band combustion gas absorption model to solve for the temperature of the high-temperature combustion gases, improving the accuracy of the concentration calculation.

The application belongs to the technical field of fluorescent probe detection, and discloses a bimetallic nanocluster fluorescent probe compound and application thereof in preparation of a product for rapidly detecting acetylene. The fluorescent probe compound is a gold-copper bimetallic nanocluster solution modified by adamantane thiol, which is used as a fluorescent probe for acetylene detection. The solution has a clear and controllable preparation process. Through specific proportioning and reaction conditions of cuprous chloride, adamantane thiol, tetrachloroauric acid, sodium borohydride and other reagents, a probe solution with stable fluorescence characteristics can be prepared. The bimetallic nanocluster fluorescent probe compound is applied in preparation of a product for rapidly detecting acetylene. Through specific combination of fluorescent molecules and acetylene gas to trigger the secondary effect of fluorescence quenching, detection is realized, and other substances cannot trigger quenching. At a wavelength of 700 nm, the linear relationship (R 2 >0.99) between the fluorescence intensity and the acetylene concentration realizes accurate quantification of the acetylene concentration, and the minimum detection limit is 0.026 muL / L.

This invention discloses a method for preparing and analyzing trans-4-aminocyclohexylacetic acid. The preparation method is as follows: 4-nitrophenylacetic acid undergoes catalytic hydrogenation in a solvent, followed by filtration. The filtrate is then treated with acid to obtain a 4-aminophenylacetic acid salt solution. The collected catalyst is activated. The 4-aminophenylacetic acid salt solution is mixed with isopropanol, barium hydroxide, tetrabutylammonium bromide, 18-crown ether-6, and dibutyl phthalate. The activated catalyst is then added to initiate a catalytic hydrogenation reaction. After the reaction, the reaction solution is cooled and filtered. The resulting solid is mixed with a preheated dilute sulfuric acid solution and stirred to form a slurry. The mixture is then filtered, and the filtrate is neutralized and filtered again. The collected filtrate is concentrated and crystallized. The crystallized precipitate is washed and dried to obtain trans-4-aminocyclohexylacetic acid. The method provided by this invention has high reaction efficiency, mild conditions, and is suitable for industrial production. It yields high product purity and reliability. Furthermore, the analytical method provided by this invention is accurate and reliable.

The application provides a die cutting and hot stamping equipment intelligent state monitoring and fault diagnosis system and method, and relates to the field of intelligent monitoring.The method comprises the following steps: collecting working parameters of multiple key parts of the die cutting and hot stamping equipment, constructing a time sequence collection window, slidingly collecting the working parameters, and obtaining characteristic information reflecting the equipment state; based on the characteristic information, constructing an anomaly detection model, performing anomaly detection on the equipment state, obtaining an anomaly score, and judging whether the equipment state is abnormal according to the anomaly score; for the characteristic information judged as abnormal, constructing a fault diagnosis model based on the fault type to which the characteristic information belongs, performing fault diagnosis on the equipment state, and generating a diagnosis result; and generating a comprehensive diagnosis and operation and maintenance decision report according to the diagnosis result.The application realizes comprehensive perception of the internal state of a closed host through multi-sensor collaborative monitoring and dynamic time sequence collection, breaks through the limitations of traditional monitoring, and provides accurate data basis for early fault warning and predictive maintenance.

This invention belongs to the field of fluorescent detection materials technology, specifically disclosing an open-type colorimetric fluorescent covalent organic polymer and its preparation and application. The preparation includes the following steps: dissolving 4,4',4''-(1,3,5-triazine-2,4,6-triyl)tris(2,6-dimethylaniline) and trialdehyde-resorcinol in 1,4-dioxane; uniformly adding acetic acid via ultrasonication; degassing three times using a refrigerated pump followed by heating reaction; sequentially washing with tetrahydrofuran and dichloromethane using a Soxhlet washing method; drying; passing through a 200-mesh sieve; and dry grinding. This invention utilizes the aforementioned open-type colorimetric fluorescent covalent organic polymer and its preparation and application. This open-type colorimetric fluorescent covalent organic polymer reacts sensitively with aminourea and 3,5-dinitro-2-hydroxyphenylenehydrazine, and can be used in open-type fluorescent chemical sensors to reduce the influence of other impurities on the detection results, thereby improving the detection sensitivity.

This invention discloses a humanoid dexterous fingertip tactile sensor, belonging to the field of tactile sensor technology. It includes a fingertip shell, a flexible contact layer, a signal acquisition unit, an encapsulation layer, and three-dimensional force-sensitive units. The fingertip shell is connected to the flexible contact layer, forming a receiving cavity between them. The signal acquisition unit, the encapsulation layer, and multiple sets of three-dimensional force-sensitive units are disposed within the receiving cavity. The signal acquisition unit includes a mainboard, an FPCB board, and Hall sensors. The number of sets of three-dimensional force-sensitive units is the same as the number of Hall sensors. Each three-dimensional force-sensitive unit includes a carrier plate, a magnet, and an elastic column. The advantages of this invention are that, in the application scenario of humanoid dexterous fingertips, this humanoid dexterous fingertip tactile sensor combines the comprehensive advantages of high three-dimensional force sensitivity, real-time feedback, high spatial resolution, miniaturized integration, and suitability for mass production.

This invention relates to the field of electroplating solution analysis technology, and in particular to a rapid analytical method for polyethylene glycol (PEG) additives in copper electrodeposition solutions. The method involves protonated PEG forming a macromolecular complex colloid with phosphomolybdate under acidic conditions of pH 1.0–2.0. This colloid exhibits maximum absorption at 372 nm, and the absorbance value is directly proportional to the PEG concentration in the formed complex colloid. This allows for quantitative analysis of PEG in the copper electrodeposition solution. Because the phosphomolybdate-PEG complex colloid selected in this invention has excellent selectivity, and no sample pretreatment is required, this invention offers advantages such as ease of operation, speed, high sensitivity, and good selectivity. It is particularly suitable for intermediate control analysis in production environments, such as for electroplated copper interconnects in chips and printed circuit boards, as well as electrolytic copper foil for negative electrode current collectors and copper-clad laminates in energy storage batteries.

The application discloses a kind of multi-element fusion carotid vascular monitoring method, system, electronic equipment and storage medium, it is related to medical monitoring field.The method comprises: obtaining the time-domain acoustic signal of carotid artery of the individual to be measured, and the time-domain acoustic signal is carried out frequency domain transformation, and frequency-domain acoustic signal is obtained;Nonlinear flow state feature and topological form feature are extracted based on the frequency-domain acoustic signal;The nonlinear flow state feature and the topological form feature are compared with the corresponding baseline feature in the historical acoustic baseline of the individual to be measured, and feature deviation result is obtained;Risk assessment is carried out based on the feature deviation result, when the feature deviation result exceeds preset range, risk early warning is triggered.The application suppresses the interference such as power frequency, baseline drift, respiratory body movement and environmental noise mixed in time-domain waveform, thereby reducing the false positive rate of carotid vascular acoustic monitoring, and realizing early risk early warning of vascular lesion.

The invention discloses a visual prompting method and system oriented to interaction of an image and a real scene, and relates to the technical field of image interaction. The visual prompting method and system for interaction between the image and the real scene comprises the following steps: S1, preprocessing real-time sensing data and task interaction historical data; s2, constructing a spatial structure expression system, and partitioning complex image information; s3, performing multi-modal image feature fusion extraction analysis on the real-time sensing data, improving the image quality, and entering an obstacle recognition and feedback process; s4, based on priority optimization and feedback adjustment evaluation, according to a grading evaluation result, identifying and marking obstacles and feeding back intensity; and S5, carrying out space reachability and risk detection on task interaction historical data and real-time perception data, and carrying out visual linkage. The technical problem that an image recognition system in the prior art is difficult to effectively recognize invisible obstacles and provide accurate real-time feedback in a deep structure and a complex environment is solved.

The invention discloses a three-dimensional pulmonary nodule target detection method and system based on a neural network. The method comprises the steps of obtaining a pulmonary nodule three-dimensional CT image and preprocessing the image to obtain a training set and a test set; a target detection model eFATE-Net is constructed, and training and evaluation are carried out; and preprocessing a to-be-detected three-dimensional CT image, and inputting the preprocessed to-be-detected three-dimensional CT image into the trained eFATE-Net to realize target detection. A false positive reduction module is introduced into the eFATE-Net, so that false positive misinformation of pulmonary nodules is reduced on the premise of keeping high detection sensitivity. On the basis of the fine-grained spatial features and the coarse-grained semantic features of the pulmonary nodules, global dependency modeling is carried out through a self-attention mechanism to reconstruct and enhance the deep semantic features of the pulmonary nodules so as to further reduce false positive and false alarm of the pulmonary nodules. A gating attention mechanism is introduced to improve key feature selectivity, the response of the model to key features of the pulmonary nodule is enhanced, and the detection performance of the pulmonary nodule is improved.

The application discloses a kind of vehicle biological invasion intelligent monitoring methods based on multi-sensor fusion, it is related to vehicle-mounted intelligent safety monitoring and response technical field, including the multi-modal data of vehicle key area is synchronously collected by sensor, and pretreatment is carried out, obtain the multi-modal data after pretreatment;Feature extraction is carried out to the multi-modal data after pretreatment, extract frequency domain feature vector, voiceprint matching degree and vibration energy increment, and the confidence index of biological invasion is comprehensively calculated;According to confidence index stratified trigger response strategy, complete the vehicle biological invasion intelligent monitoring and response based on multi-modal perception.The application significantly improves the detection sensitivity and identification accuracy of small biological invasion in vehicle key area;Give consideration to low-power on-line operation and real-time protection needs, and reduce false alarm and miss risk by stratified response.

The application discloses a two-dimensional acceleration sensor based on a ring-shaped weak coupling structure and relates to the technical field of weak vibration signal detection. The two-dimensional acceleration sensor comprises a center support structure, a ring-shaped weak coupling structure, a cantilever beam plus mass sensitive detection structure and a piezoelectric excitation and detection unit. The center support structure supports the ring-shaped weak coupling structure through elastic spokes, the ring-shaped weak coupling structure is composed of an inner ring, an outer ring and a coupling beam connecting the two, the piezoelectric excitation unit is arranged on the inner ring for generating in-plane traveling wave vibration, and a plurality of cantilever beam plus mass sensitive units are symmetrically distributed along the outer ring in the circumferential direction. The application constructs a ring-shaped weak coupling vibration energy network, realizes the propagation and redistribution of vibration energy among the plurality of sensitive units, separates the excitation and detection regions in space, effectively improves the weak acceleration detection sensitivity, reduces excitation crosstalk and realizes the decoupling detection of two-dimensional acceleration, and is suitable for the fields of high-precision inertial measurement and weak vibration monitoring.

The present disclosure provides a reagent and a method for detecting paraquat in herbicide preparation, the reagent comprising reagent A, reagent B and reagent C, the reagent A being a solution prepared from dipotassium hydrogen phosphate and an alkaline buffer, the reagent B being methanol, and the reagent C being a solution of acetylphenylhydrazine. The method comprises mixing the herbicide preparation to be tested with the reagent A to form a uniform solution A, then adding the reagent B to the uniform solution A, shaking and waiting for phase separation, taking the supernatant B and adding it to the reagent C, immediately visually comparing the color or using a spectrophotometer to compare the color, and determining whether the herbicide preparation to be tested contains paraquat according to the color change. The present disclosure uses acetylphenylhydrazine as a reducing agent combined with solvent to control the redox potential of the reducing agent, develops a detection system specific for reducing paraquat, and applies it to the detection of latent ingredients in herbicide preparations, which has the characteristics of high detection sensitivity, convenient operation and obvious phenomenon, and is suitable for on-site detection.

This invention belongs to the field of biosensor technology, specifically relating to an aflatoxin B1 sensor based on exchange catalysis and photocurrent polarity reversal, its preparation method, and its application. The sensor includes a reference electrode, a photosensitive substrate electrode, an AFB1-specific aptamer-trigger chain conjugate, a basement exchange catalytic nucleic acid amplification system, and an h-Zn1Cd5S-hairpin H conjugate. The sensor constructed by this invention is easy to operate, has a fast response speed, good repeatability, high detection sensitivity, and specificity. It can avoid interference from signal fluctuations, and its preparation method is simple, showing broad application prospects in aflatoxin B1 detection.

The invention discloses a plasma electrospray fusion ionization source and mass spectrum combined system and a detection method. The plasma electrospray fusion ionization source and mass spectrum combined system comprises a coaxially nested electrospray needle, a sample injector, an ultrahigh voltage generator, a grounded metal grid mesh, a mass spectrometer and a detector thereof. A grounded metal grid is installed between a spray needle and a mass spectrum inlet, voltage is applied to the spray needle, carrier gas is excited to generate plasmas, the macroscopic plasmas at the front end of the spray needle are fused with electrospray, and the plasmas and micro-droplets at the tip of the spray needle are fused. According to the unique ionization source constructed by the plasma-micro-droplet fusion system, on one hand, the ionization efficiency is improved, mass spectrum signals are enhanced, the application range of analytes is widened, excellent sensitivity to high-polarity compounds is kept, and effective ionization and detection of low-polarity compounds are achieved; on the other hand, higher energy and active species are provided for the micro-droplet reaction, the energy barrier required by traditional synthesis is broken, and efficient spontaneous product generation of the micro-droplet chemical reaction is achieved.

This invention relates to the field of biomedical technology, specifically to HCBP6 monoclonal antibodies and their applications. This invention utilizes bioinformatics databases and online tools to analyze the structure of the HCBP6 protein, screening for highly immunogenic HCBP6 antigen peptides. Antibody 1 and Antibody 2 are obtained through immunization. The combination of Antibody 1 and Antibody 2 is used for the detection of HCBP6, exhibiting high sensitivity and good detection results. It has significant development and application value and long-term implications for the auxiliary diagnosis of fatty liver.

The invention discloses a multi-label smell description prediction method, and relates to the field of compound smell prediction, and the method comprises the steps: obtaining compound identification information, molecular structure descriptors and smell label data, and constructing a multi-label smell data set; generating a molecular structure feature vector through a molecular fingerprint coding technology, and extracting a multi-dimensional descriptor reflecting the physicochemical properties of molecules; compressing the molecular fingerprint features to a low-dimensional space through a dimension reduction algorithm; performing unbalanced data processing on the training set, fusing the dimension-reduced molecular fingerprints with the molecular descriptors to form a joint feature matrix, and configuring a class weight balance mechanism and overfitting suppression parameters by adopting a multi-label classification architecture; independently optimizing a probability threshold for each odor label based on the verification set; and outputting a multi-odor label combination prediction result according to the target molecule identification information. According to the scheme, the multi-odor characteristics of the compound can be accurately depicted, and the combined recognition accuracy of the compound odor is remarkably improved.

The application discloses a kind of live detection devices of pole-mounted transformer internal insulation defects, including acquisition terminal, telescopic insulating rod, intelligent analysis terminal;The first side of acquisition terminal is provided with acoustic sensor and is used to adsorb to the surface of pole-mounted transformer adsorption component, and acoustic sensor is used to collect voiceprint partial discharge signal;The top of insulating rod is provided with connecting component for disassembling acquisition terminal;Intelligent analysis terminal is connected with acquisition terminal, for whether there is insulation defect in pole-mounted transformer according to voiceprint partial discharge signal analysis inside.The above-mentioned live detection device of pole-mounted transformer internal insulation defect can quickly and conveniently contact live detection to pole-mounted transformer, avoid the interface effect of box and air and air absorption attenuation, improve the anti-electromagnetic interference ability, accurately determine the internal insulation fault of transformer, improve detection sensitivity and accuracy, eliminate security risks as soon as possible, avoid transformer explosion.

The utility model is suitable for the technical field of methane telemeters, and provides a novel off-axis handheld laser methane telemeter which comprises a laser telemetering module, a shell assembly and a handheld assembly. The laser telemetering module comprises a photoelectric shell, a receiving lens and a main control board; a detection laser emission module and an indication laser emission module are arranged in the photoelectric shell, and the detection laser emission module and the receiving lens are installed at an included angle of 10 degrees; the shell assembly comprises a shell body, a sighting telescope and a display screen. The handheld assembly comprises a handle body, a key and a battery; the handle body is connected with the shell body; the button is arranged on the handle body, and a battery is further arranged in the handle body. The device is high in precision detection and strong in portability and durability; the whole machine is light in weight and can be operated by a single hand; and maintenance can be facilitated through a modular structure.

The application relates to a surface enhanced Raman scattering (SERS) substrate, a preparation method and application thereof, and belongs to the technical field of Raman spectrum detection. The substrate comprises, from bottom to top, a substrate layer, a distributed Bragg reflector, a chalcogen phase change material functional layer and a nanoscale metal structure layer. The distributed Bragg reflector is composed of a periodic structure stack of medium materials with different refractive indexes. The distributed Bragg reflector is coupled with the nanoscale metal structure layer to excite a Tamm state. The chalcogen phase change material functional layer can realize reversible regulation of amorphous state and crystalline state under external light excitation, changes the Tamm state resonance wavelength to regulate the SERS enhancement effect. The application realizes high-sensitivity detection through double enhancement of the Tamm state and localized surface plasmon resonance, has the characteristics of non-volatility, reconfigurability, high reproducibility and design flexibility, is convenient to operate, stable in performance, and is suitable for fields of chemical biological sensing, laboratory chip systems and optical encryption.

The application belongs to the technical field of biomedical signal processing and computer vision, and specifically discloses a child emotion analysis method and system based on eye movement and micro-expression association, which comprises the following steps: synchronously acquiring an eye movement data stream and a facial video stream, triggering a dynamic analysis process by identifying a mode conflict between high physiological arousal and low emotional expression intensity after generating a physiological arousal and emotion hypothesis through preliminary analysis, generating a guide strategy according to a conflict degree, and enhancing original data in a time domain or a space domain to facilitate deep analysis, capturing suppressed micro-expression signals in the enhanced data to correct the preliminary emotion hypothesis, and finally fusing a physiological arousal level, a corrected emotion category and a quantified expression inhibition degree to output a multi-dimensional psychological state analysis result containing an emotion category, a physiological arousal level and an expression inhibition degree, so as to realize quantitative and multi-dimensional evaluation of real emotions of children.

This invention relates to a miniature photoacoustic probe and monitoring system for in-situ monitoring of dissolved gases in oil. The miniature photoacoustic probe includes a pump fiber, a pump fiber ferrule, a probe fiber, an acoustic transducer film, and an oil-gas separation membrane. The pump fiber ferrule has an inner hole, and the light-emitting end of the pump fiber is positioned at a predetermined distance from the first side of the acoustic transducer film, forming a gas photoacoustic cavity in the local space between the inner hole and the ferrule. The pump fiber ferrule also has a gas guiding channel for introducing the gas to be measured, separated by the oil-gas separation membrane, into the gas photoacoustic cavity. The end face of the probe fiber and the second side of the acoustic transducer film form a Photonic Optical Interference Cavity (FP). The pump light excites the gas to be measured within the gas photoacoustic cavity, generating photoacoustic pressure. The acoustic transducer film vibrates under pressure, causing changes in the FP optical interference cavity. This probe can shorten the gas diffusion path and the sound pressure transmission path, improve the response speed, and facilitate in-situ miniaturized gas detection.

The present disclosure provides an ultrasonic fingerprint readout circuit and an ultrasonic fingerprint chip, and relates to the technical field of semiconductors. The ultrasonic fingerprint readout circuit comprises: a piezoelectric transducer configured to convert a return wave into a return wave voltage, the piezoelectric transducer comprising a pixel plate; a pixel integration module comprising a first switch assembly and a pixel integration operational amplifier, the first switch assembly being configured to reset the pixel plate by the pixel integration operational amplifier after the piezoelectric transducer sends an ultrasonic wave; a bidirectional integration module comprising a second switch assembly and a gain differential operational amplifier, the gain differential operational amplifier being configured to perform bidirectional integration based on a return wave integration voltage; and a sampling and quantization module configured to perform sampling and quantization processing on positive-phase differential signals and inverted-phase differential signals before and after bidirectional integration, so as to generate a target signal. According to the technical scheme of the present disclosure, the pixel integration operational amplifier can have dual functions of resetting the pixel plate and outputting the return wave integration voltage.

The present application relates to the technical field of gene mutation detection, and particularly relates to a system, method and application for detecting exon skipping mutation.The present application constructs a self-catalytic system based on specific double recognition induction of CRISPR-Cas12a, which is used for sensitive and reliable detection of MET 14 exon skipping mutation of lung cancer patients.The system can effectively release the fluorescence signal through the Cas12a auxiliary cutting, and further realize signal amplification through the self-catalytic mechanism.The present application can solve the technical problems of the existing exon skipping mutation detection system, such as complex detection process, long time consumption, and unsatisfactory detection accuracy.The detection system of the present application has high sensitivity, stability and good reproducibility, and the method has low cost, simple operation, short detection period, good specificity, and low false positive rate and false negative rate.The present application is suitable for clinical sample determination and has an ideal application prospect.

The invention relates to a tumor-related DNA methylation marker combination, which is selected from at least one of the following: a target sequence as shown in SEQ ID NO: 1-SEQ ID NO: 10, a nucleic acid fragment with the length of at least 15 oligonucleotides or a complementary nucleic acid fragment thereof, and the nucleic acid fragment comprises at least one methylation site indicated by CG. The invention also relates to a detection kit for the methylation marker combination. According to the invention, the combination of the co-methylation states of a plurality of specific methylation regions is used for distinguishing and analyzing the occurrence of tumors, especially kidney cancer, the specific methylation combination has high sensitivity for distinguishing the occurrence of kidney cancer, and the detection method is simple, convenient and feasible. The primer pair combination of the kit overcomes the defect of false positive caused by detection mismatch of a single methylation site in the aspect of primer sequence design, and the interaction between a plurality of methylation biomarker primers and probe pair combinations is considered.

The application discloses a particulate matter sensor and an air treatment device. The particulate matter sensor comprises a shell, a gas flow passage for a to-be-detected gas containing particulate matter is formed in the shell; a gas flow generating piece is in communication with the gas flow passage, the gas flow generating piece can drive the to-be-detected gas to pass through the gas flow passage, and a preset wind speed V of the gas flow generating piece satisfies 0.5 m / s≤V≤3 m / s; and an optical detection assembly is arranged in the shell, the optical detection assembly is used for generating a detection light beam, the detection light beam forms a light detection area at an intersection with the gas flow passage, the light detection area has a preset area S, S satisfies 16 mm 2 ≤S≤100 mm 2 , and the optical detection assembly outputs an electrical signal about a particulate matter concentration value according to the detection light beam scattered by the particulate matter.