This invention relates to a real-time monitoring method and
system for
drug testing of brain organoids, comprising the following steps: S1, preparation and pre-culture of brain organoids; S2, preparation of
microelectrode array chips and reaction microchambers: the
chip is installed in the reaction microchamber, the reaction microchamber having a
petal-shaped fluid channel structure in the center, with the center being the sample cavity, and the outer
petal channels connected to the inlet and outlet channels; S3, loading and fixing of brain organoids: after placing the brain
organoid at the center of the lower
microelectrode array electrodes, the upper
microelectrode array
chip is placed, so that the brain
organoid is clamped between the upper and lower electrodes, ensuring sufficient contact without causing pressure damage; S4,
drug perfusion and real-time electrophysiological monitoring: the
drug is introduced into the
petal-shaped fluid channel along the flow channel and diffused into the reaction cavity, and the changes in the electrical signals of the brain
organoid are recorded in real time; S5,
system cleaning and reuse.