The invention discloses a walnut source DPP-IV
inhibitory peptide as well as a preparation and
screening method and application thereof. The walnut source DPP-IV
inhibitory peptide comprises FPAG and / or LPSYQPTP, the preparation and
screening method comprises the following steps: (1) adding walnut
protein powder into deionized water, and fully dissolving to obtain a walnut
protein solution; (2) adding hydrolyzed compound
protease into the walnut
protein solution for enzymolysis to obtain walnut protein
hydrolysate freeze-dried
powder; (3) re-suspending the walnut protein
hydrolysate freeze-dried
powder in deionized water, and performing
ultrafiltration separation by using an
ultrafiltration membrane with the molecular weight cutoff of 1kDa to obtain a component F1; step (4), performing
peptide fragment sequence identification on the obtained F1 component through peptidomics; and step (5), screening from
peptide fragment sequences obtained by identification through a
bioinformatics technology. The walnut source DPP-IV
inhibitory peptide screened by the invention can be used for preparing a
medicine or food with a DPP-IV inhibition function, so that the problems that the existing walnut source DPP-IV inhibitor has many side effects and the like are solved.