The invention provides a method for surface-enhanced
Raman scattering spectrum detection by using a silver-surface
molecularly imprinted polymer, and relates to the method for the surface-enhanced
Raman scattering spectrum detection by using a
molecularly imprinted polymer. The invention aims at solving intricate process and poor detection effect problems caused by poor combination between the
molecularly imprinted polymer and a
metal film in an existing method which needs to mix the molecularly imprinted
polymer and other substances such as
silver colloid or coat the molecularly imprinted
polymer on other
metal films such as a
silver film. The method provided by the invention comprises the following steps of: I preparing
silver particles of 500 nanometers to 5 microns; II, modifying the surface of the
silver particles; III, preparing pre-assembled solution of target molecules and functional monomers; IV, preparing pre-polymerized solution of the molecularly imprinted
polymer of the silver; V, preparing the particles of silver-core molecularly imprinted membrane shells; VI, preparing the silver-surface molecularly imprinted polymer; and VII, performing the Raman detection. The method provided by the invention is applied to the field such as clinical
medicine analysis, quantitative analysis,
trace analysis and single-molecule level.