The invention discloses a nanometer silver colloid solution and a preparation method thereof. The nanometer silver colloid solution is characterized in that the 100 ml of nanometer silver colloid solution comprises the following raw materials of 0.1 to 10 g of complexing agents, 0.1 to 2 g of reducing agents, 0 to 5 g of dispersing agents, 0 to 8 g of protective agents, PH (Potential Of Hydrogen) conditioning agents for performing conditioning on the PH value within the range of 4 to 10, 0.002 to 0.4 g of silver salts and the allowance purified water. The complexing agents, the reducing agents, the dispersing agents, the protective agents, the PH conditioning agents and the silver salts are dissolved in water according to the sequential order, the aging reaction is performed under a certain condition, and the nanometer silver colloid solution is obtained eventually. According to the preparation method, the severe reduction reaction is not required, the mild polyol weak reducing agents are utilized, the reaction rate is controlled by the complexing agents, and the nanometer silver colloid solution is obtained at the low temperature. According to the nanometer silver colloid solution which is prepared from the preparation method, the controllable range of the PH value is 4 to 10 and the nanometer silver colloid solution is colorless, transparent, discoloration-resistant, strong in stability and the like. The preparation method has the advantages of being simple in operation, wide in parameter adjustable range, low in energy consumption, short in cycle, suitable for mass production and the like.