238 results about "Antibacterial action" patented technology

The invention discloses a hydrogel dressing with a double-slow-release effect as well as a preparation method and application of the hydrogel dressing. The preparation method of the hydrogel dressing comprises the following steps: firstly, preparing methyl acrylate modified hyaluronic acid capable of being photo-crosslinked, then preparing carboxymethyl chitosan nanoparticles loaded with chlorogenic acid, and finally, mixing the methyl acrylate modified hyaluronic acid and the carboxymethyl chitosan nanoparticles loaded with chlorogenic acid to obtain the hydrogel dressing. The hydrogel dressing embedded with the nanoparticles is obtained in a photo-crosslinking mode. The hydrogel dressing provided by the invention not only has good biocompatibility and mechanical properties, but also plays an anti-oxidation role by removing free radicals in a body, and can effectively kill bacteria such as escherichia coli and staphylococcus aureus to play an antibacterial role. The hydrogel dressing can also reduce excessive inflammatory response in the wound healing process by influencing macrophage polarization, promote chronic wound healing and relieve pain of patients.

The invention discloses a porous CoFe hydrotalcite nano material as well as a preparation method and application thereof, and relates to the field of nano materials. The porous CoFe hydrotalcite nano-material is of a porous layered double hydroxide nano-structure, and the aperture of the porous CoFe hydrotalcite nano-material ranges from 2 nm to 10 nm. The porous CoFe hydrotalcite nano material is prepared through a microwave hydrothermal synthesis method, the preparation method is simple, easy to operate and environmentally friendly, and industrial production can be achieved. The porous CoFe hydrotalcite nano material prepared by the method is stable in structure, has photocatalytic activity and peroxidase-like activity, can effectively catalyze and generate various reactive oxygen free radicals under the illumination condition, realizes efficient synergistic killing of bacteria, and has a good antibacterial effect.

The invention discloses a bacteriophage lyase and application thereof, and particularly relates to a bacteriophage lyase with an amino acid sequence as shown in SEQ INNO.2. The bacteriophage lyase can inhibit the growth of escherichia coli, staphylococcus and salmonella, can be used as an antibacterial substance in splitting gram-negative bacteria and can be used for preparing drugs for resisting gram-negative bacteria. The lyase can be used independently or compounded with other substances, the use concentration of antibiotics can be reduced by combining the lyase with the antibiotics, toxic and side effects (such as renal toxicity of polymyxin) of drugs are reduced, and meanwhile, generation of bacterial drug resistance is delayed. After the lyase and the chitosan are combined for use, remarkable antibacterial activity is generated, the antibacterial effect can be achieved without pre-treatment on bacteria, and the practicability is improved.

The invention discloses composite hydrogel with a photo-thermal antibacterial effect and a preparation method of the composite hydrogel. The composite hydrogel is prepared from the following components: gold nanorods with a photo-thermal effect; silver nanoparticles grow on the surface of a gold nanorod through a reduction deposition method, and a silver-coated gold nanorod of a core-shell structure is formed; oxidized hyaluronic acid and carboxymethyl chitosan are subjected to Schiff base reaction and cross-linking to form a self-healing hydrogel matrix; the advantages of the Au-Ag-PEG nanoparticles and the hydrogel are combined, so that the Au-Ag-PEG hydrogel has an excellent antibacterial effect in vitro and in vivo; therefore, the nano composite hydrogel wound dressing has huge application potential in the aspect of treating infectious skin wounds.

The invention discloses a bacteriophage-loaded gelatin hydrogel membrane as well as a preparation method and application thereof. The hydrogel film comprises the following raw materials: gelatin, glycerol and bacteriophage, the mass ratio of the gelatin to the glycerol is 1: (0.05-0.3), and the content of the bacteriophage in the hydrogel film is 106-107 PFU / g. Through physical encapsulation of the gelatin hydrogel film, the activity of the bacteriophage is effectively protected, the survival time of the bacteriophage in the storage process is prolonged, controllable slow release of the bacteriophage is achieved, and the continuous antibacterial effect of the bacteriophage on the surface of food is ensured.

The invention belongs to the field of biological agriculture, and particularly relates to bacillus amyloliquefaciens as well as a fungicide and application thereof. The invention discloses bacillus amyloliquefaciens B26 which is preserved in China General Microbiological Culture Collection Center on November 6, 2024, and the preservation number is CGMCC No.32519. The strain has a broad-spectrum antibacterial effect on fungal diseases and bacterial diseases, particularly has a remarkable antagonistic effect on tobacco wildfire germs, and can be used for preparing a bacillus amyloliquefaciens strain. The bacillus amyloliquefaciens B26 is used for preventing and treating the tobacco wildfire disease, it is found that the strain has an obvious preventing and treating effect on the tobacco wildfire disease, and a fungicide of the bacillus amyloliquefaciens B26 also has a good preventing and treating effect on the tobacco wildfire disease in a pot experiment. And a new scheme is provided for plant disease prevention and treatment, especially for green prevention and treatment of tobacco wildfire.

The invention discloses a copper-based nano antibacterial preparation and a preparation method thereof, and relates to the field of nano antibacterial materials. The copper-based nano-antibacterial preparation with high activity, broad-spectrum antibacterial effect, long acting and safety is designed by using an in-situ reduction strategy, the copper-based nano-antibacterial preparation is composed of NH2-UiO-66 (Zr) as a carrier and copper oxide nano-particles formed through copper source pre-adsorption and reducing agent reduction, then the copper oxide nano-particles are coated and modified by an amphipathic drug auxiliary material, the size is in a range of 50-500nm, and the copper-based nano-antibacterial preparation is prepared by using the amphipathic drug auxiliary material to prepare the copper-based nano-antibacterial preparation. The copper content is 5%-40% wt, the compound shows a remarkable antibacterial effect on six clinical drug-resistant bacteria, and the effective antibacterial concentrations are 20 ppm and 60 ppm respectively. Compared with copper oxide and cuprous oxide with the same molar concentration, the antibacterial effects of the antibacterial preparation on escherichia coli and staphylococcus aureus are improved by 8 times and 13 times or above respectively. The antibacterial preparation provided by the invention solves the problem of high dosage of ion antibiosis, and provides an optional way for prevention and treatment of drug-resistant bacterium infection.

The invention provides a sunlight-excited multi-ROS (reactive oxygen species) response oxygen sensitization type hydrogel. The sunlight-excited multi-ROS response oxygen sensitization type hydrogel is prepared from a plant-derived nano capsule-like body (NT), an AQPP liposome (AL) and pluronic F127 serving as a hydrogel matrix. The invention also provides a preparation method of the hydrogel and application of the hydrogel in preparation of medicines for treating skin ulcers. The NTALG causes bacterial membrane function damage after illumination, has a strong antibacterial effect and can promote healing of diabetic wounds.

The application relates to the packaging technical field and discloses a high-barrier antibacterial food packaging film and a preparation method.The raw materials of the packaging film of the application include 100 parts by weight of polyvinyl alcohol, 2-8 parts by weight of modified polyvinyl alcohol, 5-30 parts by weight of carboxymethyl chitosan and 5-20 parts by weight of sodium-based montmorillonite;the modified polyvinyl alcohol contains quaternary ammonium salt as an antibacterial group, cooperatively plays an antibacterial role with the carboxymethyl chitosan and significantly improves the antibacterial performance of the packaging film.The modified polyvinyl alcohol introduces a quaternary ammonium salt cation structure with smaller steric hindrance at the side chain end, facilitates ion exchange of the montmorillonite, modifies the polyvinyl alcohol molecular chain on the surface of the montmorillonite, improves the compatibility and dispersibility of the montmorillonite in the polyvinyl alcohol packaging film and is beneficial to improving the mechanical strength and gas barrier performance of the packaging film.

The present invention belongs to the technical field of adsorbents, and particularly relates to a modified porous adsorbent protective powder, a composite deodorant and their applications. The modified porous adsorbent protective powder of the present invention is prepared according to the following steps: dispersing an inorganic porous material dispersion or a β-cyclodextrin organic porous material dispersion, and spray-drying to obtain a porous material powder; dissolving hemicellulose and / or polypeptide in an ionic liquid to obtain a mixed solution with a mass concentration of 2-5%; adding the porous material powder into the mixed solution in the previous step, stirring and mixing evenly, filtering, adding water, and performing spray-drying to obtain the modified porous adsorbent protective powder. The present invention provides a modified porous adsorbent protective powder, which plays a role in protecting the effective active ingredients against antibacterial and deodorizing. The composite deodorant prepared by the method of the present invention has greatly improved stability and functional persistence of antibacterial and deodorizing effects.

The invention relates to the technical field of medical gel preparation, in particular to propolis temperature-sensitive in-situ gel as well as a preparation method and application thereof. A gel matrix is screened by adopting a single factor test, a prescription is optimized by adopting a star point design-effect surface method, and the quality of the propolis temperature-sensitive gel is evaluated. The optimal prescription of the propolis temperature-sensitive vaginal gel obtained through experimental optimization is as follows: 22.9% of poloxamer 407, 1.8% of poloxamer P188, 5.4% of polyethylene glycol 400 and 5% of propolis, the gelling temperature before dilution is 25 DEG C, and the gelling temperature after dilution is 37 DEG C. Quality evaluation results show that the antibacterial agent is stable in property, proper in gelling temperature and good in antibacterial activity. The prepared propolis temperature-sensitive vaginal gel is stable in process, uniform in appearance, controllable in quality and good in slow-release effect and antibacterial effect.

The invention discloses a targeted anti-aging microneedle drug delivery system for chronic wound repair as well as a preparation method and application of the targeted anti-aging microneedle drug delivery system. The system is composed of functionalized nanoparticles and a microneedle platform: a nano drug-loading unit which takes a polydopamine nanosphere as a carrier, entraps an anti-aging drug Bem, and modifies the surface of the nano drug-loading unit with a targeting peptide E7; according to the micro-needle delivery unit, hyaluronic acid-nitrogen carboxymethyl chitosan hydrogel is used as a matrix, a nano drug-loading unit and silver ions (Ag) are loaded, a micro-needle array is formed, and minimally invasive transdermal drug delivery is achieved. According to the invention, multiple mechanisms of anti-aging (APCs aging inhibition), antioxidation (ROS removal), antibiosis (Agaction) and immunoregulation (macrophage M2 type polarization promotion) are synergistically exerted, and the bottleneck problems of single intervention target, low drug availability and difficulty in regulating and controlling pathological microenvironment of chronic wounds (such as diabetic foot ulcer) in the traditional therapy are effectively solved.

The present invention relates to a skin improvement cosmetic composition comprising extracts of Polygala tenuifolia, Angelica dahurica, and Elsholtzia splendens. The cosmetic composition exhibits excellent effects of alleviating skin inflammation and providing antioxidants, skin antibacterial action and skin moisturization, and thus can be used as a cosmetic for improving the skin and, further, as a pharmaceutical composition for treating inflammatory skin diseases.

The invention relates to the field of oral cleaning products, in particular to oral antibacterial paste capable of deeply resisting bacteria and a preparation method of the oral antibacterial paste. According to the technical scheme, the incision smearing healing medicine capable of achieving rapid treatment is prepared from 15-25 parts of hydrated silica, 53-67 parts of sorbitol, 5.1-9.1 parts of water, 1.5-2.5 parts of cocamidopropyl betaine and 4-6 parts of glycerin, pathogenic bacteria are rapidly killed through triclosan, the micro-ecology is continuously adjusted through probiotics and plant extracts, the risk of drug resistance is reduced, and the healing effect is good. The plant anti-inflammatory components in the traditional Chinese medicine mixed extract are combined with the glycerol, so that red and swollen gums are relieved, wound healing is accelerated, then cocamidopropyl betaine is used for mildly cleaning oral mucosa, assisting in removing food residues and reducing bacterial attachment, and the anti-inflammatory effect is achieved. The toothpaste can deeply clean dead corners such as gingival gaps and deep mucous membranes, and can deeply play a role in the interior by virtue of an anti-bacteria and decomposition technology, so that the problem that a common oral product is limited in cleaning effect and only can clean the surface is solved.

The invention discloses a small molecule compound, a water-soluble fluorescent probe as well as a preparation method and application of the water-soluble fluorescent probe, and belongs to the technical field of medicines. According to the invention, a small molecule compound NQL containing a naphthalimide fluorophore is synthesized, and then a water-soluble fluorescent probe CS-NQL is prepared by forming Schiff base and coupling the Schiff base with chitosan oligosaccharide. The CS-NQL has environmental viscosity responsiveness and lysosome targeting specificity, so that tumor and inflammatory cell imaging can be carried out in a visible light range. The CS-NQL and DNA are subjected to electrostatic self-assembly to form nanoparticles CS-NQL coated DNA NPs, and the nanoparticles CS-NQL coated DNA NPs can generate active oxygen under an anoxic condition; under the irradiation of 660 nm laser, HeLa cell apoptosis can be induced, and an antibacterial effect is shown. An in-vivo experiment shows that CS-NQL-coated DNA NPs shows a strong anti-tumor immune response under the irradiation of 660 nm laser, so that the tumor is completely eradicated.

Modified polycaprolactone, zinc oxide and DMF are mixed to prepare a spinning solution, then the spinning solution is subjected to electrostatic spinning, drying and cutting, the tourniquet is prepared, modified polycaprolactone contains a quaternary ammonium salt structure, quaternary ammonium salt can adsorb bacteria with negative electricity through positive ions of the quaternary ammonium salt, and the antibacterial function is achieved. According to the tourniquet disclosed by the invention, zinc oxide is added into modified polycaprolactone, a hydrophobic group is inserted into a lipoid layer of a cell membrane, so that bacterial envelope dissolution and content release are caused, and further bacterial death is caused, zinc oxide can generate reactive oxygen free radicals when the tourniquet is illuminated, and further the bacterial cell membrane is damaged, so that the tourniquet has a very good antibacterial effect; according to the present invention, the cyclodextrin as the sacrificial conformation is embedded in the molecular skeleton, the conformation of the cyclodextrin is changed when the material is subjected to the external force so as to consume the energy, and the hyperbranched distribution of the polysiloxane chain segment and the polycaprolactone substantially improves the mechanical property of the material so as to improve the durability of the tourniquet;

This invention provides a long-lasting antibacterial spray, its preparation method, and its application. The antibacterial spray comprises a polyphenol adhesion component, metal ions, auxiliary polyphenols, a network stabilizer, and water. The metal ions form a dynamically reversible coordination network with the polyphenol adhesion component; the metal ions include silver ions and zinc ions. In this invention, the silver nanoparticles may undergo surface oxidation or coordination dissociation processes in the polyphenol network environment, thus serving as a potential source of supplementary silver ions, contributing to a smoother ion release behavior and achieving long-lasting antibacterial action through ion-nano synergy. This invention utilizes the synergistic effect of silver and zinc ions to improve the physical stability of the system. By introducing a small amount of silver nanoparticles as "solid-phase nodes" in the polyphenol network, the structural stability and resistance to mechanical damage of the antibacterial system are improved without significantly increasing the total silver release.

The invention provides a medical functional coating, a preparation method and a implantation intervention medical device. The preparation method of the medical functional coating comprises the following steps: obtaining meso-porous silicon nanoparticles loaded with curcumin; adding the meso-porous silicon nanoparticles loaded with the curcumin into a solution of carboxymethyl chitosan and sodium alginate to form a gel solution of the meso-porous silicon nanoparticles loaded with the curcumin; providing a base material, and adsorbing the gel solution on the surface of the base material to form a coating capable of releasing curcumin; tissue regeneration after prostatic hyperplasia resection can be promoted, the antibacterial effect is achieved, the pain of a patient is relieved, and the treatment recovery period is shortened.

The invention discloses an alpha-aminophosphoryl derivative as well as a preparation method and application thereof. The method comprises the following steps: by taking a quinoline derivative as a raw material and tetrahydrofuran (THF) as a solvent, adding boron trifluoride diethyl etherate in a nitrogen system at the reaction temperature of 0 DEG C, stirring for 15 minutes, reducing the temperature of the reaction system to T1 (-60 DEG C to-30 DEG C), adding a Grignard reagent into the reaction system, and reacting for 30 minutes to obtain a solution A; dissolving a phosphorus reagent in the organic solvent at room temperature in a nitrogen atmosphere to obtain a solution B; and adding the solution A into the solution B, reacting for 6-15 hours at the reaction temperature T2 (0-50 DEG C), and after the reaction is finished, extracting, concentrating and carrying out column chromatography to obtain the target product alpha-aminophosphoryl derivative. The invention provides a novel synthetic route for preparing the alpha-aminophosphonyl derivative, and various phosphorus reagents such as diaryl phosphorus oxide and phosphite ester can be compatible with the reaction. The alpha-aminophosphoryl derivative synthesized by the invention has a good antibacterial effect on gram-positive bacteria, especially staphylococcus aureus.

The invention belongs to the technical field of medical chemistry, and discloses a 5-O-carbon mould amine tylosin lactone derivative with a double-target combined antibacterial effect as well as a preparation method and application of the 5-O-carbon mould amine tylosin lactone derivative. The derivative is a compound as shown in a formula I, and pharmaceutically acceptable salt, eutectic, tautomer, polymorphic substance, solvate, prodrug or isotope labeled compound thereof. The compound can act on a bacterial ribosome 50S subunit and DNA topoisomerase IV at the same time, and shows an excellent antibacterial effect in an antibacterial activity test, and the minimum inhibitory concentration (MIC) reaches 0.0625 mu g / mL. The compound has the advantages of being high in antibacterial activity, wide in antibacterial spectrum and capable of achieving rapid sterilization, and a new choice is provided for developing drugs with antibacterial activity.

The invention relates to an application of a combined composition nanoparticle in preparation of a medicine for resisting fungal and bacterial infection. The active components of the combined composition nanoparticle are antibiotics and antibiotic adjuvants, the antibiotic adjuvant is tripterine or a pharmaceutically acceptable salt thereof. Compared with the prior art, the tripterine or the medicinal salt thereof and the antibiotics are prepared into the nanoparticles by utilizing a nanotechnology, so that the solubility of the fat-soluble antibiotics is effectively improved, the biocompatibility of the tripterine or the medicinal salt thereof is improved, and the tripterine or the medicinal salt thereof has an excellent effect in resisting fungal and bacterial infection. The tripterine or the medicinal salt thereof can enhance the antibacterial effect, especially the antifungal effect, of the antibiotics, increase the antibacterial sensitivity of the antibiotics and inhibit the occurrence of fungal or bacterial drug resistance.

The present application relates to the technical field of microbial pesticides, in particular to a deep-sea streptomyces with antifeedant function and antibacterial effect and application thereof. The streptomyces from the South China Sea deep sea is Streptomyces sp. NA13, which was preserved in China Center for Type Culture Collection on August 29, 2022, and the preservation number is CCTCC M 20221343. The strain has the ability to prepare antibiotics such as Antimycin, Candicidin, Naringenin, Surugamide, Fredericamycin, SAL-2242, Albaflavenone, Hopene, SGR PTMs, etc. In particular, the fermentation is used to prepare antimycin or cyclic octapeptide surugamide compounds, including novel antimycin compounds antimycin Q (1). The prepared antimycin compounds are used as insect pest antifeedants or antifungal drug lead compounds, and have application prospects of developing into natural source antibacterial and insecticidal pesticides.

The invention discloses a chitosan / lysozyme amyloid protein fiber composite microneedle based on ethephon controlled release and application of the composite microneedle in on-demand ripening acceleration of harvested fruits, and belongs to the technical field of agricultural product after-harvest treatment. The composite microneedle is composed of chitosan, lysozyme amyloid protein fibers and ethephon, the mass ratio of the chitosan to the lysozyme amyloid protein fibers is 5: 5, and the ethephon loading capacity is that 131.13 [mu] L of a 40% ethephon solution is added to every 17.5 mL of a 1% chitosan solution. The microneedle can be inserted into the epidermis of picked fruits to realize on-demand slow release of ethephon and promote ripening of the fruits, meanwhile, the lysozyme amyloid protein fiber provides an antibacterial effect, the aging process of the fruits after ripening is effectively delayed, the infection rate of colletotrichum gloeosporioides is reduced, and the ethephon residue in the fruits is remarkably lower than the national limited standard. The method solves the problems of non-uniform fruit ripening, excessive softening and potential toxicity caused by a traditional ethephon ripening method, and realizes integration of batch on-demand ripening and preservation of picked fruits.

The invention discloses ethanol-promoted bacteriostatic hydrogel which is prepared by the following steps: (1) taking sodium alginate, N-isopropylacrylamide and methacrylated hyaluronic acid as substrate materials, adding glycerol and distilled water, uniformly mixing, adding acetic acid to adjust the pH value to be neutral, adding a photoinitiator, uniformly mixing, and performing ultraviolet curing to obtain primary gel; and (2) soaking the primary gel in an ethanol solution containing plant antibacterial components for secondary crosslinking, and cleaning with absolute ethyl alcohol to obtain the ethanol-promoted antibacterial hydrogel. The preparation method is simple and easy to implement, and the hydrogel is long in bacteriostatic action time and remarkable in bacteriostatic effect.

This invention discloses a multi-target synergistic preservation method for fruits and vegetables based on the active ingredients of Torreya grandis aril, belonging to the field of fruit and vegetable preservation technology. The method includes the following steps: Step 1: Using Torreya grandis aril as raw material, Torreya grandis aril essential oil is extracted by steam distillation. This multi-target synergistic preservation method for fruits and vegetables based on the active ingredients of Torreya grandis aril combines four active ingredients—3-carene, D-limonene, δ-juniperol, and δ-juniperene—from the Torreya grandis aril essential oil with chitosan to construct an integrated synergistic preservation system of "antioxidant—enzyme inhibition—film protection—antibacterial action." This system protects the integrity of fruit cell membranes and delays softening and water loss, thereby overcoming the shortcomings of existing technologies that are single-function and unstable in effect. It achieves precise, multi-target intervention across the entire chain of postharvest physiological decline in perishable fruits and vegetables.

The invention relates to an anti-inflammatory plasma matrix as well as a preparation method and application thereof. The anti-inflammatory plasma matrix comprises a plasma matrix and aspirin chitosan nanoparticles, the preparation method of the anti-inflammatory plasma matrix comprises the step of pressing a mixture of the plasma matrix and the aspirin chitosan nanoparticles to obtain the anti-inflammatory plasma matrix. Leukocyte contained in the plasma matrix in the anti-inflammatory plasma matrix can play an antibacterial role, aspirin can be slowly released by the aspirin chitosan nano-particles, the function of regulating and controlling bone regenerative cycle in an inflammatory state is achieved, and a chitosan shell also has certain antibacterial and anti-inflammatory effects. The prepared anti-inflammatory plasma matrix can be applied to preparation of drugs for treating periodontitis and peri-implantitis.

The present invention provides a class of compounds that can be used for textile antibacterial treatment, as well as their preparation methods and uses, and belongs to the field of medical material technology. The present invention develops a compound with high photosensitivity, the structure of which is shown in Formula I. The compound has advantages such as good modifiability, good biocompatibility, strong bioadhesion and adsorption capacity, and easy binding to cell membranes. It can effectively adsorb nucleic acids or proteins of bacteria or viruses, and can effectively fight bacteria without light treatment. After light treatment, it has a better antibacterial effect and shows excellent antibacterial effect against both Gram-positive and Gram-negative bacteria. Textiles treated with the compound of the present invention have a certain hydrophobicity, can inhibit bacterial adhesion, exert excellent antibacterial effects, are simple to operate, low cost, and are suitable for antibacterial treatment of various textiles, with broad application prospects.

The invention discloses a pesticide composition containing emamectin benzoate and application of the pesticide composition in prevention and treatment of rice blast, and belongs to the technical field of pesticides. The pesticide composition comprises 1-5 parts of emamectin benzoate, 1-3 parts of cinnamon essential oil and 0.5-1.5 parts of orange peel essential oil. Adding polyvinyl alcohol into the heated deionized water, adding glycerol, and uniformly stirring to obtain a water phase; adding the pesticide composition into soybean oil, and then adding alkyl glycoside and phenolic ether phosphate to obtain an oil phase; under high-speed shearing, adding the oil phase into the water phase, continuously shearing, finally adding a defoaming agent, stirring and filtering to obtain the pesticide for preventing and treating the rice blast. The emamectin benzoate is compounded with the cinnamon essential oil and the orange peel essential oil, and the orange peel essential oil not only has an antibacterial effect, but also has a rapid permeation effect, so that permeation of the cinnamon essential oil can be accelerated, and volatilization of the cinnamon essential oil is reduced; components such as a film-forming agent are added into the auxiliary agent, so that a film is quickly formed after the pesticide is sprayed, and the volatilization of the cinnamon essential oil is further prevented; the control effect on the rice blast is obviously improved.

The invention relates to the technical field of natural product chemistry and cosmetics, and particularly discloses application of a composition containing a radix salviae miltiorrhizae extract in preparation of a product with an acne removing effect. The composition containing the radix salviae miltiorrhizae extract comprises the radix salviae miltiorrhizae extract and a calendula officinalis extract, the radix salviae miltiorrhizae extract is obtained by extracting with an ethanol water solution; the calendula officinalis extract is extracted by a carbon dioxide supercritical extraction method. Researches show that the composition containing the salvia miltiorrhiza extract, which is obtained by combining the salvia miltiorrhiza extract prepared by the method and the calendula officinalis extract, has a certain antibacterial effect on propionibacterium acnes. Therefore, the composition has important application value when being used as an active ingredient for preparing the medicine or the cosmetic with the acne removing effect.

The present invention relates to a method for treating Gram-negative bacteria that are resistant to colistin (MIC > 50 mg / mL), an antibacterial compound, excluding those of the genera Burkholderia, Proteus, and Serratia, comprising contacting the bacteria with an aqueous pharmaceutical composition containing a rose bengal (RB) compound of formula I, dissolved or dispersed at a concentration of about 0.01 to about 15 mg / mL, and irradiating the contacted bacteria with light of a wavelength of about 500 nm to about 600 nm for a period of about 1 to about 10 minutes, at a concentration of about 16 to about 160 J / cm². 2 The aim is to create a method that involves obtaining a certain amount of light to treat and kill irradiated bacteria. [Formula 1] JPEG2026511692000028.jpg5864 (where X, R 1 , R 2 (and M+ are defined herein)