328 results about "Antibacterial action" patented technology

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide and application thereof. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO: 1, the number of amino acids is small, the synthesis cost is low, the antibacterial peptide has high antibacterial activity on gram-negative bacteria and gram-positive bacteria, the minimum inhibitory concentration of the antibacterial peptide on acinetobacter baumannii, escherichia coli, pseudomonas aeruginosa and staphylococcus aureus is 4.68-37.5 mu g / mL, and the antibacterial peptide has the advantages of broad-spectrum antibacterial property, good stability and good application prospect. No obvious cytotoxicity and hemolysis effect exist; moreover, end-to-end amido bond cyclization is carried out, so that the antibacterial activity is further enhanced, the antibacterial effect on acinetobacter baumannii, escherichia coli, pseudomonas aeruginosa and staphylococcus aureus is enhanced by 2-4 times, and the minimum inhibitory concentration is reduced to 2.34-9.38 mu g / mL; after the end-to-end amido bonds are cyclized, the plasma stability and safety of the antibacterial peptide are further improved, and the cytotoxicity and hemolysis are reduced.

The invention discloses a hydrogel dressing with a double-slow-release effect as well as a preparation method and application of the hydrogel dressing. The preparation method of the hydrogel dressing comprises the following steps: firstly, preparing methyl acrylate modified hyaluronic acid capable of being photo-crosslinked, then preparing carboxymethyl chitosan nanoparticles loaded with chlorogenic acid, and finally, mixing the methyl acrylate modified hyaluronic acid and the carboxymethyl chitosan nanoparticles loaded with chlorogenic acid to obtain the hydrogel dressing. The hydrogel dressing embedded with the nanoparticles is obtained in a photo-crosslinking mode. The hydrogel dressing provided by the invention not only has good biocompatibility and mechanical properties, but also plays an anti-oxidation role by removing free radicals in a body, and can effectively kill bacteria such as escherichia coli and staphylococcus aureus to play an antibacterial role. The hydrogel dressing can also reduce excessive inflammatory response in the wound healing process by influencing macrophage polarization, promote chronic wound healing and relieve pain of patients.

The invention discloses a preparation method and application of pH-responsive double-synergistic-therapy hydrogel. The hydrogel is mainly prepared from oxidized fucoidin (OFU), carboxymethyl chitosan (CMCs), zinc peroxide / copper nanoparticles (ZnO < 2-Cu >) and arginine (L-ARG). Under a gastric acid condition, the hydrogel generates H2O2 through zinc peroxide, copper ions catalyze H2O2 through a Fenton-like reaction to generate OH <->, arginine can catalyze H2O2 to generate NO, and the copper ions and the arginine act together to destroy a helicobacter pylori biological membrane and kill bacteria. The hydrogel has good injectability, self-healing property and adhesivity, is beneficial to intragastric injection application and can be adhered to the stomach, and the administration concentration of an infected part is improved; the antibacterial effect is generated only under the gastric acid condition, the helicobacter pylori biological membrane can be destroyed, and the helicobacter pylori antibacterial agent can be applied to the helicobacter pylori which is difficult to eradicate in the stomach of the human body.

The invention discloses a porous CoFe hydrotalcite nano material as well as a preparation method and application thereof, and relates to the field of nano materials. The porous CoFe hydrotalcite nano-material is of a porous layered double hydroxide nano-structure, and the aperture of the porous CoFe hydrotalcite nano-material ranges from 2 nm to 10 nm. The porous CoFe hydrotalcite nano material is prepared through a microwave hydrothermal synthesis method, the preparation method is simple, easy to operate and environmentally friendly, and industrial production can be achieved. The porous CoFe hydrotalcite nano material prepared by the method is stable in structure, has photocatalytic activity and peroxidase-like activity, can effectively catalyze and generate various reactive oxygen free radicals under the illumination condition, realizes efficient synergistic killing of bacteria, and has a good antibacterial effect.

The invention discloses an application of osmundone in preparation of a medicine for treating methicillin-resistant staphylococcus aureus infectious pneumonia. Experimental results of an MRSA mouse pneumonia infection model show that osmundone can significantly reduce the bacterial load in lung tissues, alleviate excessive inflammatory response caused by infection and effectively improve the survival rate of mice. In addition, the invention discloses that osmundone reduces the adhesion and invasion ability of bacteria, reduces the toxicity of the bacteria and plays an antibacterial role by inhibiting the protein activity of staphylococcus aureus Sortase A (SrtA). Different from traditional antibiotics, osmundone does not apply survival pressure to bacteria, so that generation of drug resistance is not easily induced. On the basis, osmundone shows a good application prospect in the aspect of research and development of safe and effective MRSA infectious pneumonia treatment drugs.

The invention discloses a bacteriophage lyase and application thereof, and particularly relates to a bacteriophage lyase with an amino acid sequence as shown in SEQ INNO.2. The bacteriophage lyase can inhibit the growth of escherichia coli, staphylococcus and salmonella, can be used as an antibacterial substance in splitting gram-negative bacteria and can be used for preparing drugs for resisting gram-negative bacteria. The lyase can be used independently or compounded with other substances, the use concentration of antibiotics can be reduced by combining the lyase with the antibiotics, toxic and side effects (such as renal toxicity of polymyxin) of drugs are reduced, and meanwhile, generation of bacterial drug resistance is delayed. After the lyase and the chitosan are combined for use, remarkable antibacterial activity is generated, the antibacterial effect can be achieved without pre-treatment on bacteria, and the practicability is improved.

The invention discloses multifunctional nano-enzyme composite hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: synthesizing molybdenum disulfide MoS2 by taking hexaammonium heptamolybdate tetrahydrate and thiourea as raw materials; the preparation method comprises the following steps: carrying out stirring reaction on MoS2 and cysteine to obtain MoS2-LC; the preparation method comprises the following steps: mixing galangin with MoS2-LC, carrying out ultrasonic treatment, and carrying out a stirring reaction to obtain MoS2-LC (at) Gal; the preparation method comprises the following steps: adding acetic anhydride into a carboxymethyl chitosan aqueous solution to obtain N-acetylated carboxymethyl chitosan N-CC; the preparation method comprises the following steps: adding 1-butyl-3-methylimidazole chlorine salt into N-CC to react, and carrying out alcohol precipitation to obtain N-CC-IL; a cross-linking agent, MoS2-LC (at) Gal and N-CC-IL are subjected to a blending reaction, and SNCI (at) MLG is obtained. Pharmacodynamic results show that the multifunctional nano-enzyme composite hydrogel prepared by the preparation method disclosed by the invention can play a role in inhibiting ferroptosis while having an antibacterial effect, and a new possibility is provided for periodontal local treatment.

The invention discloses composite hydrogel with a photo-thermal antibacterial effect and a preparation method of the composite hydrogel. The composite hydrogel is prepared from the following components: gold nanorods with a photo-thermal effect; silver nanoparticles grow on the surface of a gold nanorod through a reduction deposition method, and a silver-coated gold nanorod of a core-shell structure is formed; oxidized hyaluronic acid and carboxymethyl chitosan are subjected to Schiff base reaction and cross-linking to form a self-healing hydrogel matrix; the advantages of the Au-Ag-PEG nanoparticles and the hydrogel are combined, so that the Au-Ag-PEG hydrogel has an excellent antibacterial effect in vitro and in vivo; therefore, the nano composite hydrogel wound dressing has huge application potential in the aspect of treating infectious skin wounds.

The invention discloses a bacteriophage-loaded gelatin hydrogel membrane as well as a preparation method and application thereof. The hydrogel film comprises the following raw materials: gelatin, glycerol and bacteriophage, the mass ratio of the gelatin to the glycerol is 1: (0.05-0.3), and the content of the bacteriophage in the hydrogel film is 106-107 PFU / g. Through physical encapsulation of the gelatin hydrogel film, the activity of the bacteriophage is effectively protected, the survival time of the bacteriophage in the storage process is prolonged, controllable slow release of the bacteriophage is achieved, and the continuous antibacterial effect of the bacteriophage on the surface of food is ensured.

The present invention relates to a composition for a food container and a method for manufacturing a food container by using same and, more specifically, to a composition for a food container and a method for manufacturing a food container by using same, wherein the composition comprises zeolite, an antibacterial additive, and a porous adsorption powder to adsorb and remove ethylene gas, carbonic acid gas, and the like generated by respiration of vegetables and fruits and to prevent the decay caused by microorganisms through an antibacterial action, thereby keeping vegetables and fruits fresh for a long time.

The invention belongs to the field of biological agriculture, and particularly relates to bacillus amyloliquefaciens as well as a fungicide and application thereof. The invention discloses bacillus amyloliquefaciens B26 which is preserved in China General Microbiological Culture Collection Center on November 6, 2024, and the preservation number is CGMCC No.32519. The strain has a broad-spectrum antibacterial effect on fungal diseases and bacterial diseases, particularly has a remarkable antagonistic effect on tobacco wildfire germs, and can be used for preparing a bacillus amyloliquefaciens strain. The bacillus amyloliquefaciens B26 is used for preventing and treating the tobacco wildfire disease, it is found that the strain has an obvious preventing and treating effect on the tobacco wildfire disease, and a fungicide of the bacillus amyloliquefaciens B26 also has a good preventing and treating effect on the tobacco wildfire disease in a pot experiment. And a new scheme is provided for plant disease prevention and treatment, especially for green prevention and treatment of tobacco wildfire.

The invention belongs to the technical field of biomedicine, and particularly relates to a Chinese mouse ear-derived antibacterial peptide and application thereof. The antibacterial peptide derived from the Chinese mouse ear is MC-CATH1, MC-CATH2 or MC-CATH3, and the antibacterial peptide derived from the Chinese mouse ear is MC-CATH3; the antibacterial peptides MC-CATH1, MC-CATH2 and MC-CATH3 derived from the hepialus sinensis have relatively strong antibacterial activity on gram positive bacteria and gram negative bacteria, have broad-spectrum and efficient antibacterial action, and have the characteristics of low hemolytic activity, small cytotoxicity, salt tolerance and strong antibacterial biofilm activity, so that the antibacterial peptides MC-CATH1, MC-CATH2 and MC-CATH3 can be used for preparing the antibacterial peptides of the hepialus sinensis, and the antibacterial peptides MC-CATH1, MC-CATH2 and MC-CATH3 can be used for preparing the antibacterial peptides of the hepialus sinensis. In addition, the MC-CATH3 can kill bacteria by inducing the bacteria to generate a ferroptosis-like mechanism. The MC-CATH1, the MC-CATH2 and the MC-CATH3 can be applied to the fields of medicines, cosmetics, food preservation and the breeding industry.

The invention relates to the field of biological medicine, in particular to a polypeptide composition with bacteriostatic activity and a preparation method thereof.The polypeptide composition is prepared by wrapping polypeptide I with modified polyethylene glycol, then wrapping with nano-silver and finally modifying polypeptide II with the nano-silver. The outer layer is sequentially wrapped with spherical particles of modified polyethylene glycol, nano-silver and polypeptide II, and the polypeptide II and the nano-silver are promoted to be combined with a bacterial membrane and destroy the bacterial membrane through the non-specific electrostatic interaction of the polypeptide II and the nano-silver, so that the polypeptide II has relatively high antibacterial performance; the outer-layer polypeptide is degraded by proteolytic enzyme or plays a role with a bacterial membrane, so that the inner-layer polypeptide I wrapped by the modified polyethylene glycol is exposed, the antibacterial effect is further played, and the proteolytic stability of the polypeptide is improved; due to the spherical structure, the prepared polypeptide composition with the antibacterial activity has excellent antibacterial performance, meanwhile, the stability can be improved, and the polypeptide composition has wide application prospects.

The invention discloses a copper-based nano antibacterial preparation and a preparation method thereof, and relates to the field of nano antibacterial materials. The copper-based nano-antibacterial preparation with high activity, broad-spectrum antibacterial effect, long acting and safety is designed by using an in-situ reduction strategy, the copper-based nano-antibacterial preparation is composed of NH2-UiO-66 (Zr) as a carrier and copper oxide nano-particles formed through copper source pre-adsorption and reducing agent reduction, then the copper oxide nano-particles are coated and modified by an amphipathic drug auxiliary material, the size is in a range of 50-500nm, and the copper-based nano-antibacterial preparation is prepared by using the amphipathic drug auxiliary material to prepare the copper-based nano-antibacterial preparation. The copper content is 5%-40% wt, the compound shows a remarkable antibacterial effect on six clinical drug-resistant bacteria, and the effective antibacterial concentrations are 20 ppm and 60 ppm respectively. Compared with copper oxide and cuprous oxide with the same molar concentration, the antibacterial effects of the antibacterial preparation on escherichia coli and staphylococcus aureus are improved by 8 times and 13 times or above respectively. The antibacterial preparation provided by the invention solves the problem of high dosage of ion antibiosis, and provides an optional way for prevention and treatment of drug-resistant bacterium infection.

The invention provides a sunlight-excited multi-ROS (reactive oxygen species) response oxygen sensitization type hydrogel. The sunlight-excited multi-ROS response oxygen sensitization type hydrogel is prepared from a plant-derived nano capsule-like body (NT), an AQPP liposome (AL) and pluronic F127 serving as a hydrogel matrix. The invention also provides a preparation method of the hydrogel and application of the hydrogel in preparation of medicines for treating skin ulcers. The NTALG causes bacterial membrane function damage after illumination, has a strong antibacterial effect and can promote healing of diabetic wounds.

The application relates to the packaging technical field and discloses a high-barrier antibacterial food packaging film and a preparation method.The raw materials of the packaging film of the application include 100 parts by weight of polyvinyl alcohol, 2-8 parts by weight of modified polyvinyl alcohol, 5-30 parts by weight of carboxymethyl chitosan and 5-20 parts by weight of sodium-based montmorillonite;the modified polyvinyl alcohol contains quaternary ammonium salt as an antibacterial group, cooperatively plays an antibacterial role with the carboxymethyl chitosan and significantly improves the antibacterial performance of the packaging film.The modified polyvinyl alcohol introduces a quaternary ammonium salt cation structure with smaller steric hindrance at the side chain end, facilitates ion exchange of the montmorillonite, modifies the polyvinyl alcohol molecular chain on the surface of the montmorillonite, improves the compatibility and dispersibility of the montmorillonite in the polyvinyl alcohol packaging film and is beneficial to improving the mechanical strength and gas barrier performance of the packaging film.

The preparation method comprises the following steps: grinding an iodine block to obtain iodine powder, adding the iodine powder into double distilled water, and carrying out ultrasonic stripping to obtain an iodine nanosheet suspension; under the conditions of stirring and water bath heating, dissolving polyvinyl alcohol with double distilled water to obtain a polyvinyl alcohol solution, adding sodium alginate in batches, and continuously stirring in a water bath to obtain a moldable composite hydrogel precursor solution; dissolving tannic acid by adopting an iodine nanosheet suspension, then adding into the moldable composite hydrogel precursor solution, uniformly stirring at room temperature, adjusting the pH value of the solution to be neutral, finally dropwise adding a borax solution, and stirring into gel to obtain the I2 / PBST moldable antibacterial composite hydrogel. The prepared composite hydrogel not only can play an antibacterial role and reduce the bacterial infection probability of infection sites, but also has excellent properties such as plasticity, stretchability, self-healing property, adhesion and degradability, is convenient to fill defect parts with different shapes, and is firm in adhesion.

The present invention belongs to the technical field of adsorbents, and particularly relates to a modified porous adsorbent protective powder, a composite deodorant and their applications. The modified porous adsorbent protective powder of the present invention is prepared according to the following steps: dispersing an inorganic porous material dispersion or a β-cyclodextrin organic porous material dispersion, and spray-drying to obtain a porous material powder; dissolving hemicellulose and / or polypeptide in an ionic liquid to obtain a mixed solution with a mass concentration of 2-5%; adding the porous material powder into the mixed solution in the previous step, stirring and mixing evenly, filtering, adding water, and performing spray-drying to obtain the modified porous adsorbent protective powder. The present invention provides a modified porous adsorbent protective powder, which plays a role in protecting the effective active ingredients against antibacterial and deodorizing. The composite deodorant prepared by the method of the present invention has greatly improved stability and functional persistence of antibacterial and deodorizing effects.

The invention relates to the technical field of medical gel preparation, in particular to propolis temperature-sensitive in-situ gel as well as a preparation method and application thereof. A gel matrix is screened by adopting a single factor test, a prescription is optimized by adopting a star point design-effect surface method, and the quality of the propolis temperature-sensitive gel is evaluated. The optimal prescription of the propolis temperature-sensitive vaginal gel obtained through experimental optimization is as follows: 22.9% of poloxamer 407, 1.8% of poloxamer P188, 5.4% of polyethylene glycol 400 and 5% of propolis, the gelling temperature before dilution is 25 DEG C, and the gelling temperature after dilution is 37 DEG C. Quality evaluation results show that the antibacterial agent is stable in property, proper in gelling temperature and good in antibacterial activity. The prepared propolis temperature-sensitive vaginal gel is stable in process, uniform in appearance, controllable in quality and good in slow-release effect and antibacterial effect.

The invention discloses a targeted anti-aging microneedle drug delivery system for chronic wound repair as well as a preparation method and application of the targeted anti-aging microneedle drug delivery system. The system is composed of functionalized nanoparticles and a microneedle platform: a nano drug-loading unit which takes a polydopamine nanosphere as a carrier, entraps an anti-aging drug Bem, and modifies the surface of the nano drug-loading unit with a targeting peptide E7; according to the micro-needle delivery unit, hyaluronic acid-nitrogen carboxymethyl chitosan hydrogel is used as a matrix, a nano drug-loading unit and silver ions (Ag) are loaded, a micro-needle array is formed, and minimally invasive transdermal drug delivery is achieved. According to the invention, multiple mechanisms of anti-aging (APCs aging inhibition), antioxidation (ROS removal), antibiosis (Agaction) and immunoregulation (macrophage M2 type polarization promotion) are synergistically exerted, and the bottleneck problems of single intervention target, low drug availability and difficulty in regulating and controlling pathological microenvironment of chronic wounds (such as diabetic foot ulcer) in the traditional therapy are effectively solved.

The present invention relates to a skin improvement cosmetic composition comprising extracts of Polygala tenuifolia, Angelica dahurica, and Elsholtzia splendens. The cosmetic composition exhibits excellent effects of alleviating skin inflammation and providing antioxidants, skin antibacterial action and skin moisturization, and thus can be used as a cosmetic for improving the skin and, further, as a pharmaceutical composition for treating inflammatory skin diseases.

The invention relates to the technical field of artificial lenses, in particular to a modified artificial lens and a preparation method and application thereof. The modified intraocular lens is characterized in that a PHEMA-copolymer film-serine polymer intraocular lens is used as a carrier, and adriamycin is loaded on the surface of the carrier. The modified intraocular lens has good physical barrier and biological inhibition effects, can avoid accumulation of proteins and lipids in tears, can avoid generation of bacterial infection complications, can prevent adhesion of lens epithelial cells, can block DNA replication and transcription processes of the lens epithelial cells, can inhibit proliferation of the lens epithelial cells, and can improve the lens epithelial cell proliferation rate. The prepared nano-material can induce apoptosis of lens epithelial cells, has an excellent antibacterial effect and good biocompatibility, and can prevent the occurrence of posterior cataract.

The invention relates to the field of oral cleaning products, in particular to oral antibacterial paste capable of deeply resisting bacteria and a preparation method of the oral antibacterial paste. According to the technical scheme, the incision smearing healing medicine capable of achieving rapid treatment is prepared from 15-25 parts of hydrated silica, 53-67 parts of sorbitol, 5.1-9.1 parts of water, 1.5-2.5 parts of cocamidopropyl betaine and 4-6 parts of glycerin, pathogenic bacteria are rapidly killed through triclosan, the micro-ecology is continuously adjusted through probiotics and plant extracts, the risk of drug resistance is reduced, and the healing effect is good. The plant anti-inflammatory components in the traditional Chinese medicine mixed extract are combined with the glycerol, so that red and swollen gums are relieved, wound healing is accelerated, then cocamidopropyl betaine is used for mildly cleaning oral mucosa, assisting in removing food residues and reducing bacterial attachment, and the anti-inflammatory effect is achieved. The toothpaste can deeply clean dead corners such as gingival gaps and deep mucous membranes, and can deeply play a role in the interior by virtue of an anti-bacteria and decomposition technology, so that the problem that a common oral product is limited in cleaning effect and only can clean the surface is solved.