The invention discloses
propolis gauze for treating
osteoarthritis as well as a preparation method and application of the
propolis gauze. The invention belongs to the technical field of
medicine. The preparation method comprises the following steps: weighing a
propolis crude extract, adding a 70wt%
ethanol solution according to a
solid-
liquid ratio of 1: 6-1: 20, carrying out extraction on the propolis crude extract in a
shaker at 37 DEG C for 1-3 times, 24 hours each time, mixing the extraction solutions, carrying out suction
filtration, carrying out rotary
evaporation on the filtrate to remove
ethanol, transferring the filtrate into a
beaker,
drying the filtrate in a
drying oven at 55 DEG C until the filtrate is pasty, and carrying out
freeze drying for 24 hours to obtain the propolis freeze-dried
powder. The method comprises the following steps: suspending propolis freeze-dried
powder by using a mixed
solvent containing 2wt%-5wt% of DMSO (dimethylsulfoxide), 10wt%-40wt% of absolute ethyl
alcohol and 10wt%-30wt% of PEG400 (
polyethylene glycol 400) to prepare a 1.25-25mg / mL propolis freeze-dried
powder solution, performing ultrasonic treatment for 30 minutes, centrifuging for 10 minutes at 4000 rpm, standing for 10 minutes at
room temperature, and taking supernate for later use;
cutting medical gauze into squares of 10cm * 10cm, folding the medical gauze into small squares of 2.5 cm * 2.5 cm, putting the small squares into a medical
plastic bottle, pouring the supernatant into the
bottle to submerge the gauze, and covering and sealing the
bottle. During application, a piece of propolis gauze is taken to be unfolded and is applied to the affected part of
arthritis for 1-4 hours. The propolis gauze provided by the invention is applied to the treatment of
osteoarthritis.