465 results about "Antibacterial peptide" patented technology

The invention discloses a full D-type antibacterial peptide with high enzymolysis stability and broad-spectrum antibacterial activity and application thereof. The antibacterial peptide is obtained by sequentially and repeatedly arranging D-type tryptophan, D-type arginine and D-type lysine for four times and carrying out C-terminal amidation; the amino acid sequence of the gene is (D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-NH2, and is marked as DWRK-12. The antibacterial peptide shows broad-spectrum antibacterial activity and excellent stability, can effectively resist clinically separated multidrug resistant strains, and keeps high cell selectivity at the same time. Due to the characteristics, the antibacterial peptide has important application value in the aspect of developing novel antibacterial drugs, especially in the field of treatment of infection of multiple drug-resistant bacteria.

The invention belongs to the technical field of antibacterial peptide activity identification and evaluation, and relates to an antibacterial peptide activity and MIC value combined prediction framework based on cross-modal deep learning, and the framework uses a protein language model ESM2 to respectively carry out token-level semantic embedding coding on an antibacterial peptide sequence and a pathogen protein sequence; performing cross-modal feature extraction and fusion through a multi-branch structure comprising a Mama module, a multi-head self-attention mechanism and DASM 1D convolution; a multi-task decoding structure is adopted to realize antibacterial peptide activity classification and MIC value regression prediction at the same time; according to the method, functional characteristics in the sequence can be effectively mined, the accuracy and generalization ability of antibacterial peptide activity and MIC value prediction are remarkably improved, and a reliable calculation tool is provided for high-throughput screening and rational design of the antibacterial peptide.

The invention discloses an antibacterial peptide based on D-type amino acid and application of the antibacterial peptide, and the antibacterial peptide is characterized in that the amino acid sequence is w-r-r-w-r-r-w-w-r-k-r (dTrp-dArg-dArg-dTrp-dArg-dArg-dTrp-dTrp-dArg-dLys-dArg). The antibacterial peptide can be synthesized through an Fmoc solid-phase chemical method, the synthesis difficulty is low, and the in-vivo antibacterial activity is good. The antibacterial peptide especially has broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus, is low in hemolytic toxicity, can reach half or more of cell survival rate under medium and low concentration, almost has no toxic effect on cells, and can be used for preparing the antibacterial peptide with a broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus. The stability is good, the operability is high, and the cost is low.

The invention discloses an application of an antibacterial peptide and an application of the antibacterial peptide in preparation of antibacterial drugs for treating carbapenem-resistant pseudomonas aeruginosa, klebsiella pneumoniae (ATCC700603), escherichia coli (ATCC25922), staphylococcus aureus and methicillin-resistant staphylococcus aureus, the antibacterial peptide is characterized in that the sequence is w-r-r-w-k-r-w-w-r-r, and the sequence is shown in the description. All amino acids are D-type amino acids; the antibacterial peptide can be used as an antibacterial substance in wash supplies, food preservative additives, medical consumable antibacterial agents and cosmetics, can be synthesized through an Fmoc solid-phase chemical method, and is low in synthesis difficulty and good in in-vivo antibacterial activity. Particularly, the antibacterial peptide has broad-spectrum killing activity against carbapenem pseudomonas aeruginosa, klebsiella pneumoniae (ATCC700603), escherichia coli (ATCC25922), staphylococcus aureus and methicillin-resistant staphylococcus aureus, the cell survival rate of the antibacterial peptide within the range of medium and low concentration (2 mu g / mL-32 mu g / mL) reaches half or more, the toxicity is small, the toxicity is almost avoided, the operability is high, and the antibacterial peptide can be widely applied to the field of medical application. The cost is low.

The invention discloses lysozyme, antibacterial peptide derived from the lysozyme and application of the lysozyme in preparation of antibacterial drugs. According to the invention, 1, 640 protein sequences recorded in a ruminant rumen caterpillars genome database are systematically predicted based on a lysozyme prediction model, and finally, the lysozyme with the amino acid sequence as shown in SEQ ID No.1 is relatively strong in antibacterial activity and stable in antibacterial activity, and is slightly influenced by environmental factors. According to the invention, an antibacterial peptide region of the lysozyme is analyzed and determined, three peptide fragments with clear antibacterial activity are identified, and the amino acid sequences of the peptide fragments are respectively shown as SEQ ID No.2, SEQ ID No.3 or SEQ ID No.4. The antibacterial peptide provided by the invention has relatively strong membrane destroying ability and broad-spectrum antibacterial activity, and compared with integral protein, the antibacterial peptide is simpler in structure and more flexible in synthesis and modification, and is easier to break through a bacterial drug-resistant mechanism.

The invention belongs to the technical field of polypeptides and biological medicines, and particularly relates to a WK7 series antibacterial peptide and application thereof in the aspects of resisting bacteria and preventing and treating diabetic foot, pneumonia and septicemia. According to the invention, a linear peptide WK7-line with an amino acid sequence of WKRWKRW is used as a minimum active unit, and modification is carried out to form a plurality of linear modified peptides; meanwhile, a cyclic peptide WK7-cyl is formed on the basis of the linear peptide WK7-line through cyclization, and the cyclic peptide WK7-cyl is further modified to form an annular modified peptide. The linear peptide WK7-line, the linear modified peptide, the cyclic peptide WK7-cyl and the cyclic modified peptide jointly form the WK7-series antibacterial peptide, and the WK7-series antibacterial peptide is artificially synthesized polypeptide, is small in molecular weight, easy to synthesize, free of cytotoxicity, almost free of hemolysis risk, high in sterilization speed and broad-spectrum antibacterial property and can be used for preparing the antibacterial peptide. The traditional Chinese medicine composition has a certain effect on prevention and treatment of diabetic foot ulcer, pneumonia and septicemia.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide Mh-GC21 as well as a precursor and application thereof. The amino acid sequence of the antibacterial peptide Mh-GC21 provided by the invention is as shown in SEQ ID NO.1, and two cysteines are oxidized to form a pair of intermolecular disulfide bonds; the C tail end of the antibacterial peptide Mh-GC21 is subjected to amidation modification. The antibacterial peptide Mh-GC21 provided by the invention is from microhyla microcarpa, has broad-spectrum antibacterial activity, has relatively good antibacterial and bactericidal effects on standard strains of acinetobacter baumannii, escherichia coli and staphylococcus aureus and clinically sourced drug-resistant strains, and can be used for targeted destruction of cell membranes of bacteria, removal of biological membranes and inhibition of formation of the biological membranes. Moreover, the antibacterial peptide Mh-GC21 has the advantages of good stability, no hemolytic activity and cytotoxicity, and difficulty in inducing strains to generate drug resistance.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an LL-37 derived antibacterial peptide and application thereof. According to the invention, 17-29aa of the LL-37 antibacterial peptide is used as a core fragment and is connected with an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 through an amido bond, the antibacterial activity of the obtained antibacterial peptide is obviously higher than that of the LL-37 antibacterial peptide, and the antibacterial peptide shows an obvious bactericidal effect on clinical drug-resistant bacteria such as acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, klebsiella pneumoniae and the like; meanwhile, the cytotoxicity and hemolytic activity are weak, drug resistance is not prone to being generated, bacteria can be rapidly killed in vivo through multiple mechanisms, no obvious cytotoxicity exists, the bacterium load in tissue is remarkably reduced, and the lifetime of a bacterium-infected mouse is prolonged.

The invention relates to the technical field of PA resin, and particularly discloses glass fiber modified antibacterial PA resin suitable for children products and a preparation method of the antibacterial PA resin. The invention discloses glass fiber modified antibacterial PA resin suitable for children products. The glass fiber modified antibacterial PA resin comprises PA resin, glass fibers, talcum powder, nano calcium carbonate, a composite antibacterial agent, a compatilizer, an antioxidant and calcium stearate, the composite antibacterial agent comprises chitosan, an antibacterial peptide microcapsule and a porous silver-zinc-loaded antibacterial agent; the antibacterial peptide microcapsules are sodium alginate embedded antibacterial peptides. The antibacterial PA resin obtained in the invention has the highest bacteriostasis rates of 99.97%, 99.74% and 99.95% for staphylococcus aureus, escherichia coli and candida albicans respectively, the antibacterial property of the PA resin is improved, the compressive strength and notch impact strength are 170.6 MPa and 17.3 kJ / m < 2 > respectively, and high mechanical strength is maintained on the basis of high antibacterial property.

The invention provides an antibacterial peptide generation method constructed based on a recognition model, and the method comprises the steps: updating a dynamic data set, and carrying out the adversarial optimization and repeated iteration of a generator network and a discriminator network based on the updated dynamic data set, thereby guaranteeing the learning of real diversified antibacterial peptide features with high antibacterial activity and low toxicity, and improving the recognition efficiency of the antibacterial peptide. According to the method, the capability of identifying the real antibacterial peptide from the antibacterial peptide data set is improved, the authenticity of the representative amino acid sequence generated by the generator network is synchronously verified in adversarial tuning, and the generation efficiency of the antibacterial peptide is improved.

The invention relates to a preparation method of an integrated medical fertilizer rich in antibacterial peptide, and discloses a preparation method of an integrated medical fertilizer with sodium alginate-chitosan biphasic embedding nucleation and chemical fertilizer base material low-temperature spraying shell, by applying the method, the thallus encapsulation efficiency of sodium alginate-chitosan microcapsules reaches 85-95%, and the thallus encapsulation efficiency of the sodium alginate-chitosan microcapsules reaches 85-95%. The core-shell structure is stored for 6 months at normal temperature, the survival rate of thalli is 80-90%, the activity of the antibacterial peptide is retained by 60-80%, and compared with a sodium alginate single-layer embedding technology, the particle compressive strength has remarkable advantages in the aspects of the survival rate of the thalli and the activity retention of the antibacterial peptide.

The invention belongs to the technical field of biology, and particularly relates to an antibacterial peptide for inhibiting propionibacterium acnes and application of the antibacterial peptide. The antibacterial peptide for inhibiting propionibacterium acnes is derived from marine organism hylophila, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The amino acid sequence is novel, has remarkable antibacterial activity on propionibacterium acnes, has the advantages of low hemolysis toxicity, low cytotoxicity and high safety, and can be used for preparing the antibacterial peptide for inhibiting propionibacterium acnes. Due to the characteristics, the compound has huge application potential in the aspect of treating acnes caused by propionibacterium acnes or removing acnes.

The invention discloses an insect antibacterial peptide nutrient solution as well as a preparation method and application thereof, and belongs to the technical field of biology. Insect protein (fly maggot powder / hermetia illucens) and plant fiber (bran) are subjected to enzymolysis and then subjected to a pH gradient chelation process (5.0-7.0-8.5), metal ions (Fe < 2 + > / Mn < 2 + > / Zn < 2 + >) are added in stages for chelation, the chelation degree is larger than or equal to 91.4%, lentinan and alginate oligosaccharide are added for synergistic interaction, and finally the insect antibacterial peptide nutrient solution is obtained. Experiments prove that after the insect antibacterial peptide nutrient solution prepared by the invention is used for carrying out root irrigation treatment on plants, tomato yellow leaf curl virus or tomato spotted wilt virus can be remarkably prevented and treated, and the insect antibacterial peptide nutrient solution has high efficiency and persistence and conforms to the development direction of green plant protection.

The invention relates to the technical field of water purifiers, in particular to a filter element, a preparation method of the filter element and a water purifying device.The filter element comprises a silica sand layer, an activated carbon adsorption layer and a modified nano-silver functional layer which are stacked in the water flow direction; wherein the modified nano-silver functional layer comprises nano-silver particles modified by antibacterial peptide-cyclodextrin. According to the filter element prepared through the method, a superfine and dispersed nano-silver particle system is constructed, the stability of nano-silver in the filter element system can be greatly enhanced, then the antibacterial activity and antibacterial broad-spectrum performance of the nano-silver are improved, meanwhile, efficient removal of heavy metal is achieved in an auxiliary mode, and the water purification efficiency of a water purification device is effectively and comprehensively improved.

The invention relates to three antibacterial peptides and application thereof in resisting fungi and / or inducing plant immunity. Specifically, on one hand, the invention provides three antibacterial peptides which are novel functional antibacterial peptides ZJUAMP1, ZJUAMP2 and ZJUAMP65, the sequences of the novel functional antibacterial peptides ZJUAMP1, ZJUAMP2 and ZJUAMP65 are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3, and the novel functional antibacterial peptides have good antifungal activity, can activate an MAPK signal channel in a plant body and induce the plant to generate systematic disease resistance, and are suitable for the fields of crop disease prevention and control and plant protection. The two antibacterial peptides are simple in structure and easy to synthesize. On the other hand, the invention further provides application of the two antibacterial peptides in resisting fungi, activating a plant MAPK signal channel, inducing plant immune response, enhancing plant disease resistance and / or preventing and treating plant diseases.

The invention discloses an antibacterial peptide prediction method based on dual-channel sparse attention. The method comprises the following specific steps: extracting initial embedding by using a protein language model; adaptively weighting the channel by using the channel attention enhanced convolutional neural network; capturing short-range interactions between amino acid residues using local sparse attention based on a sliding window; global sparse attention containing a scoring function is used for obtaining long-range interaction between key amino acid residues and the sequence, and local and global information is integrated so as to realize complete characterization of the peptide sequence; and predicting the final feature matrix by using a full connection layer. According to the method, the sequence information of the peptide is comprehensively modeled by using a simple and efficient training process, and a dual-channel sparse attention mechanism is introduced to effectively relieve the problems of representation redundancy and calculation complexity.

The invention discloses an antibacterial peptide PA targeting multi-drug-resistant gram-negative bacteria and application of the antibacterial peptide PA, and the amino acid sequence of the antibacterial peptide PA is shown as SEQ ID NO.1. The antibacterial peptide PA provided by the invention has a remarkable antibacterial effect of the multi-drug-resistant gram-negative bacteria, is quick in bactericidal effect, does not have hemolysis and cytotoxicity, does not cause renal toxicity after treatment, and can be used for preparing the antibacterial peptide PA for treating the multi-drug-resistant gram-negative bacteria. The biological safety is good, and the compound can be used for treating bacterial infection and can also be applied to other scenes needing sterilization or bacterial growth inhibition.

The invention belongs to the technical field of gene engineering, and particularly relates to ALFPm3 modified antibacterial peptide, a molecular design and construction method and application of the ALFPm3 modified antibacterial peptide, and the amino acid sequence of the ALFPm3 modified antibacterial peptide is selected from any one of SEQ ID NO: 1-SEQ ID NO: 7. Analysis and design are carried out by combining the structure and action site of vibrio parahaemolyticus and the molecular structure and function relationship of the antibacterial peptide ALFPm3 as a basic template, the antibacterial activity of the antibacterial peptide ALFPm3 is improved, and other biological activities such as hemolysis are reduced.

The invention discloses an application of Micropterus salmoides Piscidin polypeptide in preparation of an anti-fish virus drug. The amino acid sequence of the polypeptide is as shown in SEQ ID NO. 2 or SEQ ID NO. 3. The research finds that the micropterus salmoides Piscidin is an antibacterial peptide with an antiviral function, the polypeptide synthesized by the artificially synthesized micropterus salmoides Piscidin has the characteristic of inhibiting fish DNA virus replication, has a remarkable inhibiting effect on fish iridovirus replication at low concentration, and can be applied to preparation, research and development of fish antiviral drugs. Research of the antibacterial peptide has important scientific value and application value for further research and development of disease-resistant functional gene products.

The invention discloses an antibacterial peptide generating and screening method based on a conditional potential diffusion model, which comprises the following steps: modeling antibacterial peptide sequence distribution in a continuous submerged space, realizing controllable generation of antibacterial peptide sequences through conditional constraints, and improving the efficiency and reliability of an antibacterial peptide discovery process by combining a multi-stage calculation screening and experimental verification strategy; therefore, the problem that in an existing antibacterial peptide design method, due to the factors that the sequence space scale is huge, the generation process is difficult to control, the candidate sequence redundancy is high, and the screening cost is high, the antibacterial peptide discovery efficiency is limited is solved. According to the method, a conditional diffusion generation mechanism is introduced into a continuous submerged space, and a multi-stage screening process formed by calculation screening and experimental verification is combined, so that systematic design and optimization of an antibacterial peptide sequence from data driven generation to performance evaluation are realized.

The invention provides a rhodopseudomonas palustris antibacterial peptide, an antibacterial product and application, and belongs to the technical field of biological prevention and treatment medicine manufacturing. According to the application disclosed by the invention, two functional short peptides, namely KKKYG and VVGRR, with remarkable fungal antagonism activity are identified from extracellular polypeptide components of rhodopseudomonas palustris for the first time. Experiments prove that the two types of oligopeptides not only can effectively inhibit the formation of magnaporthe oryzae appressorium, but also can obviously weaken the pathogenicity of the oligopeptides on rice hosts and inhibit the pathogenicity of magnaporthe oryzae on rice, and have potential broad-spectrum antagonistic fungal activity and excellent biological control potential. The functional oligopeptide liquid preparation or freeze-dried powder prepared on the basis of the method is non-toxic to people and livestock, has no phytotoxicity to crops, does not pollute the environment, and meets the core requirements of green agriculture and sustainable development; and the oligopeptide preparation shows good heat-resistant stability, is convenient to store and transport, and adapts to complex and changeable field environments.

The invention relates to the technical field of agricultural fertilizers. More specifically, the invention relates to an extraction method of tenebrio molitor antibacterial peptide and application of the tenebrio molitor antibacterial peptide in preparation of tea tree anthracnose resistant fertilizer. According to the preparation method, the colletotrichum camelliae liquid is taken as an induction factor, the ultrasonic induction technology is utilized to strengthen the absorption of tenebrio molitor to the induction factor, meanwhile, a supercritical / ethanol cosolvent extraction process and a bionic affinity chromatography purification process are combined, the efficient extraction of the antibacterial peptide is realized, and the obtained antibacterial peptide has a relatively good inhibition effect on the colletotrichum camelliae. A tea tree anthracnose resisting leaf fertilizer is further prepared through the tenebrio molitor antibacterial peptide, the leaf fertilizer has a good prevention and treatment effect on tea tree anthracnose caused by camellia colletotrichum spinosus, meanwhile, tea growth can be promoted, and the yield reduction caused by anthracnose and nutrition deficiency of tea tree crops can be effectively reduced.

This invention provides an antimicrobial peptide spray, its preparation method, and its application in the prevention and treatment of peristomal skin infections. The spray comprises, by weight percentage, 0.05%-0.5% of a complex antimicrobial peptide, 0.1%-5% of a moisturizing and repairing film-forming matrix, 0.05%-0.2% of a buffer, 0.8%-1.2% of an isotonic regulator, 0.01%-0.5% of a stabilizer, and 0.005%-0.05% of a metal ion chelating agent, with the balance being water for injection. The complex antimicrobial peptide is composed of bovine lactoferrin antimicrobial peptide, sericin oligopeptide, and buckwheat-derived anti-inflammatory active peptide in a weight ratio of (3-5):(2-3):1. This spray possesses four core functions: broad-spectrum antibacterial activity, inhibition of bacterial biofilm, anti-inflammatory and soothing effects, and skin barrier repair. It is less likely to induce bacterial resistance, does not irritate damaged skin around the stoma, and can effectively prevent and treat peristomal skin infections.

The invention provides micropterus salmoides Hepcidin-1 (Ms-Hepcidin-1) and an application of a synthetic polypeptide of the micropterus salmoides Hepcidin-1 in preparation of an anti-fish virus drug. The research finds that the Ms-Hepcidin-1 synthetic polypeptide has a remarkable antiviral effect and is a novel antibacterial peptide with a function of inhibiting fish viruses. The Ms-Hepcidin-1 synthetic polypeptide and the recombinant eukaryotic expression vector of the Ms-Hepcidin-1 synthetic polypeptide can obviously reduce the transcription level of MCP, MMP and DNMT genes and the MCP protein level of LMBV, inhibit the replication of the LMBV and reduce the virus titer, and have an obvious fish virus resisting effect. Moreover, the polypeptide synthesized by Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use and convenient to preserve, can be used as a novel antiviral functional gene product to prepare antiviral drugs for fishes, and has important significance on prevention and control of fish iridovirus diseases.

This invention discloses an antimicrobial peptide PA targeting multidrug-resistant Gram-negative bacteria and its application. The amino acid sequence of the antimicrobial peptide PA is shown in SEQ ID NO.1. The antimicrobial peptide PA provided by this invention has a significant antibacterial effect against multidrug-resistant Gram-negative bacteria, and has a rapid bactericidal onset, no hemolysis or cytotoxicity, does not cause nephrotoxicity after treatment, and has good biosafety. It can be used for the treatment of bacterial infections and can also be applied to other scenarios that require bactericidal or antibacterial growth inhibition.

The invention relates to the technical field of bioengineering, in particular to a preparation method and application of an antibacterial peptide, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID No: 1 and is named as L8. The preparation method of the antibacterial peptide L8 comprises the following steps: S1, obtaining an L8 target gene segment; connecting an L8 target gene segment with a pLC vector to obtain a connection product; transforming the connection product into an escherichia coli DH5-alpha competent cell, and screening positive clones to obtain a pLC-L8 recombinant plasmid; s2, taking the pLC-L8 recombinant plasmid as a substrate, and carrying out cell-free protein synthesis reaction to obtain the antibacterial peptide L8, the cell-free protein synthesis system comprises 175 mM of potassium glutamate, 10 mM of ammonium glutamate, 2.7 mM of potassium oxalate, 33 mM of PEP, 38 mM of compound amino acid, 10 mM of magnesium glutamate, 2% (w / v) PEG-8000, 5 nM of pLC-L8 recombinant plasmid, 11.25 mM of lactose, 33.33% (v / v) of crude extract, 100 mg / mL of escherichia coli tRNA and 2.5 nM of T7 RNA polymerase. The antibacterial peptide L8 is obtained by optimizing the antibacterial peptide LRGG, and compared with the LRGG, the antibacterial peptide L8 subjected to cell-free protein synthesis reaction expression has better antibacterial activity and biological safety.

The invention discloses a milk-derived broad-spectrum antibacterial peptide W-2 and application thereof, and relates to the technical field of biology. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID NO. 4. Symmetrical structure design and amino acid mutation are carried out on the basis of the template peptide to obtain the novel antibacterial peptide, and tests show that the novel antibacterial peptide has broad-spectrum antibacterial activity which is obviously higher than that of the template peptide, and basically has no cytotoxicity. The antibacterial peptide prepared by the invention still keeps good antibacterial activity in physiological salt concentration and protease environment, has certain serum stability, and has higher application value; in addition, the antibacterial peptide has a good inhibition effect on the formation of a bacterial biofilm, and has a synergistic effect with traditional antibiotics.

The invention discloses bacteriostatic gel for gynecology and a preparation method thereof, and relates to the technical field of bacteriostatic gel. The antibacterial gel is prepared from a gel matrix, a plant extracting solution, antibacterial peptide, a humectant, astaxanthin microcapsules and a specific buffer solution. The buffer solution contains sodium citrate, hyaluronic acid and citric acid, and intelligent regulation and control of the drug release behavior of the astaxanthin microcapsule are realized by utilizing the synergistic effect of a hyaluronic acid and citric acid buffer system and preferably adopting hyaluronic acid compounded or modified with different molecular weights. The gel can maintain stable drug release in a normal vagina and pathological fluctuation pH environment, and trigger accelerated release in a high active oxygen inflammation environment, so that the problems that the release behavior is greatly influenced by environmental fluctuation and cannot adapt to different disease stages in the prior art are effectively solved, accurate and personalized drug delivery is realized, and the drug delivery efficiency is improved. The antibacterial agent has the advantages of good antibacterial effect and high environmental adaptability.

The application belongs to the field of polypeptides, and particularly relates to a marine-derived antibacterial peptide, a mutant thereof, an antibacterial composition and application. The application screens a marine-derived antibacterial peptide, and optimizes net charge, hydrophobicity and amphiphilicity of the antibacterial peptide through mutation. Through experimental testing, the four antibacterial peptides have differential antibacterial effects on different gram-negative bacteria, wherein the antibacterial peptide mutant shown in SEQ ID NO. 2 has the best antibacterial effect and low hemolysis, and has a positive application prospect.

The application provides a compound microbial agent for preventing and treating bacterial diseases of fruits and vegetables and a preparation method thereof, wherein the compound microbial agent comprises Brevibacillus brevis, Pseudomonas chlororaphis and Streptomyces; wherein the ratio of the viable bacterial count of the Brevibacillus brevis, the Pseudomonas chlororaphis and the Streptomyces is (1-3):(2-4):(1-2). The Brevibacillus brevis directly inhibits bacteria by secreting antibacterial peptides, the Pseudomonas chlororaphis competes for ecological niches through iron carriers, and the Streptomyces produces antibiotics, so that multiple strains form a "bacteriostasis-competition-immune activation" multiple mechanism, thereby achieving efficient prevention and treatment of bacterial diseases of fruits and vegetables.