24 results about "Acinetobacter baumannii" patented technology

This invention relates to a 1,3,4-thiadiazole peptide deformylase inhibitor and its preparation and application. The structural formula is shown in formula (1): wherein, R 1 It is n-butyl or cyclopentylmethyl; R 2 The compounds are hydrogen-containing, straight-chain alkanes, cyclic alkanes, aromatic rings, substituted biphenyls, or heterocyclic rings. The 1,3,4-thiadiazole peptide deformylase inhibitors provided by this invention can effectively inhibit bacterial protein synthesis, thereby achieving sterilization. These compounds exhibit excellent inhibitory activity against Gram-positive drug-resistant bacteria, especially the clinically challenging methicillin-resistant Staphylococcus aureus (MRSA). In particular, some preferred compounds show inhibitory activity 4-8 times that of the control standards vancomycin and linezolid. The 1,3,4-thiadiazole peptide deformylase inhibitors provided by this invention also exhibit inhibitory activity against Gram-negative drug-resistant bacteria, especially against drug-resistant Acinetobacter baumannii, known as "superbugs," with an inhibitory activity reaching 0.5 μg / mL, far superior to vancomycin and linezolid.

The invention discloses a low-temperature denitrification strain and application of the low-temperature denitrification strain. The strain is Acinetobacter Bragg FX1, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO: 36583. The strain can efficiently remove ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in water at the temperature of 5-30 DEG C, especially shows the optimal denitrification performance at the temperature of about 10 DEG C, and the removal rate of the ammonia nitrogen and the nitrite nitrogen can reach 99% or above. The strain has strong adaptability to low-temperature environment, can tolerate water quality fluctuation of actual sewage, and solves the problems of low denitrification efficiency and unstable operation caused by insufficient microbial activity of sewage treatment plants in low-temperature regions. The strain can be used for preparing a low-temperature sewage denitrification microbial agent, can be directly added into a low-temperature domestic sewage or industrial wastewater treatment system, does not need to depend on high-energy-consumption heating and heat preservation measures, and has remarkable energy-saving and consumption-reducing potential, good environmental adaptability and wide application prospects.

The invention relates to the technical field of biological detection, in particular to a nucleic acid combination product, a PCR premix, a method and a kit for identifying respiratory tract pathogenic bacteria. The nucleic acid combination product comprises a primer pair as shown in SEQ ID NO: 1 to SEQ ID NO: 21 and a probe. The invention provides a nucleic acid detection product for detecting klebsiella pneumoniae, streptococcus pneumoniae, haemophilus influenzae, pseudomonas aeruginosa, acinetobacter baumannii, staphylococcus aureus and moraxella catarrhalis, and the nucleic acid detection product can be used for performing amplification detection on conserved fragments of target pathogens in the same PCR reaction system, and is efficient and low in cost. Moreover, the anti-interference capability is strong, and the sensitivity is high.

The application discloses a chimeric endolysin Lys009-Lys009CBD and engineering and application thereof, and belongs to the field of genetic engineering and protein engineering. The application constructs a chimeric endolysin Lys009-Lys009CBD with high activity, and the peptidoglycan hydrolysis activity of the chimeric endolysin Lys009-Lys009CBD on Pseudomonas aeruginosa is 5.30 times that of natural endolysin Lys009 and 27.98 times that of hen egg white lysozyme HEWL. A transmembrane peptide P81417 is further fused to perform transmembrane modification, and an engineering endolysin P81417-Lys009-Lys009CBD is obtained. The engineering endolysin can efficiently lyse Pseudomonas aeruginosa, Acinetobacter baumannii and the like, and has excellent thermal stability. The application enriches the resource library of high-activity endolysins, provides important experimental basis and technical support for developing an anti-drug-resistant bacteria treatment strategy, and also provides a new selection for clinical treatment of multiple drug-resistant bacteria infection.

The application discloses a strain of Acinetobacter, the strain name is C16ZT1, and simultaneously proposes a bacterial agent containing the different bacillus, and further uses the bacterial agent for treating petroleum pollution wastewater. The Acinetobacter can efficiently degrade the petroleum production wastewater containing chain alkanes, can still realize the degradation effect quickly and stably under the conditions of high concentration, high salt and extreme acid and alkali, and can degrade more than 95% of the petroleum production wastewater within 144 hours. In the application in sewage treatment, the strain has strong tolerance, can tolerate high-concentration alkanes, and has a wide adaptation range to salt concentration, pH and the like, and has a good application prospect.

The application discloses a kind of anti-saline-alkali microbial inoculants and preparation method thereof, belong to the field of microbial technology.The microbial inoculants is made of composite microbial inoculants 100-150 parts, fish bone hydrolysate 10-30 parts, nano silicon sol 1-3 parts.The composite microbial inoculants is made of Trichoderma reesei, Pseudomonas plecoglossicida, Acinetobacter baumannii.The anti-saline-alkali microbial inoculants has remarkable effect on saline-alkali soil improvement, greatly reduces soil acidity, salt content and bulk density, and the yield of saline-alkali soil wheat planting is obviously improved.The soil organic matter content and soil enzyme activity are significantly improved after use, the soil quality and rhizosphere microecological environment are improved, and the enzyme activity in wheat is improved, the stress resistance is improved, the yield reduction caused by saline-alkali stress is reduced, and the use of microbial inoculants can also reduce the amount of chemical fertilizer and pesticide, reduce production cost, and further improve the economic benefit of wheat planting.

The invention belongs to the technical field of bioengineering, and particularly relates to a phage cocktail and application thereof. The bacteriophage cocktail comprises five bacteriophages PGPA20, PGPA60, PAB26, Lkpp-25 and Lkpp-36 which are separated from the natural world, and the bacteriophage cocktail has relatively strong bactericidal activity on pseudomonas aeruginosa, acinetobacter baumannii and K1 / K2 type klebsiella pneumoniae, and has specificity on the pseudomonas aeruginosa, the acinetobacter baumannii and the K1 / K2 type klebsiella pneumoniae; the bacteriophage can be compounded with other substances for use, and a safe and non-toxic bacteriophage killing product is provided for in-vivo and in-vitro infection treatment of pseudomonas aeruginosa, acinetobacter baumannii and K1 / K2 type klebsiella pneumoniae and disinfection and purification of pseudomonas aeruginosa, acinetobacter baumannii and K1 / K2 type klebsiella pneumoniae in the breeding industry and other environments.

The invention discloses a carbapenem drug resistance gene detection primer group based on multiple PCR-time-of-flight mass spectrometry, a detection kit and a kit using method, and through a large number of screening and verification experiments, a multiple primer group suitable for time-of-flight mass spectrometry detection is obtained. Therefore, the multi-PCR-time-of-flight mass spectrometry detection of the multi-drug-resistant genes of carbapenem-resistant enterobacteriaceae bacteria becomes possible. The detection kit prepared by adopting the primer group can be used for simultaneously detecting and identifying 10 common carbapenem drug-resistant genes, including klebsiella pneumoniae KPC gene, NDM gene, IMP gene, VIM gene and OXA gene, acinetobacter baumannii OXA gene, NDM gene, Escherichia coli OXA gene, IMP gene and serratia marcescens VIM gene. Meanwhile, the kit is high in sensitivity and specificity, dozens of times of detection can be carried out in the same batch, so that the detection efficiency is greatly improved, and the kit is suitable for rapid screening and genetic typing of carbapenem drug-resistant bacteria in clinical samples.

The invention belongs to the technical field of microorganisms, and in particular relates to an Acinetobacter solani BP2 strain and a related application of the Acinetobacter solani BP2 strain. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 4, 2025, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.36892. The acinetobacter soilis BP2 is found to have multiple growth promoting functions of efficiently secreting indoleacetic acid, dissolving insoluble organic phosphorus and inorganic phosphorus, fixing nitrogen and producing siderophores at the same time; when applied to the plant growth process, the seed germination and growth of crops can be remarkably promoted. According to the invention, excellent strain resources are provided for soil nutrient activation and plant growth promotion, and a foundation is laid for development of efficient microbial agents.

The application discloses application of BMS-833923 and derivatives thereof and a medicine, and belongs to the technical field of antibiotics. The BMS-833923 and the derivatives thereof can be used as colistin adjuvants to jointly inhibit or eliminate gram-negative bacteria, and can also independently inhibit or eliminate gram-positive bacteria. The gram-negative bacteria can include at least one of Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, pan-drug-resistant strains BAA-1800, BAA-1794 and BAA-1792, and the gram-positive bacteria can include at least one of Staphylococcus aureus and Bacillus subtilis. By taking the BMS-833923 and the derivatives thereof as adjuvants of colistin, the therapeutic index of the colistin can be effectively expanded, and lower, non-toxic doses of the colistin can be used in clinical treatment of effective treatment of drug-resistant bacterial infections.

The application discloses a new Schumannella species with antibacterial activity and application thereof, and belongs to the technical field of microorganisms. A strain 10F1B-5-1 is identified as a potential new species of Schumannella (Schumannella), and is classified and named as Schumannella sp. The strain 10F1B-5-1 has been preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.1.16698. The Schumannella 10F1B-5-1 provided by the application has effects on gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and gram-negative bacteria (Acinetobacter baumannii), provides a strain resource for research and development of new antibiotics, and has good research value and application potential.

The application discloses specific primers for detecting Acinetobacter baylyi and application of the specific primers, and the specific primers comprise baylyi-F and baylyi-R. The primers are used for PCR amplification, and 370 bp, clear and single DNA bands are obtained through 1.2% agarose gel electrophoresis and Sanger sequencing. The accuracy rate of bacterial liquid PCR detection of the Acinetobacter baylyi is 100%, the time consumption is only 3 hours, and the method is successfully applied to conjugation transfer experiment of the Acinetobacter baylyi ADP1. The method for detecting the Acinetobacter baylyi by using the specific primers for detecting the Acinetobacter baylyi provided by the application is simple, efficient and high in accuracy.

The application discloses a strain with a red mud dealkalization function and application thereof, and the classification name of the BRY1 strain is Acinetobacter baumannii Acinetobacter baumannii , the preservation number of which is GDMCC No 66371, the preservation date is May 22, 2025, and the preservation unit is Guangdong Microbial Culture Collection Center. The BRY1 strain provided by the application has strong alkali resistance and high acid production capacity, and can quickly and effectively neutralize alkaline substances in red mud. The BRY1 strain is obtained by being separated and screened from a specific environment, i.e., a red mud storage yard, and has good adaptability and dealkalization specificity to the red mud environment.

The invention discloses a novel Schumann bacterium species with antibacterial activity and application thereof, and belongs to the technical field of microorganisms. The bacterial strain 10F1B-5-1 is identified as a potential new species of Schumannella, the classification name of the bacterial strain 10F1B-5-1 is Schumannella sp., the bacterial strain 10F1B-5-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacterial strain 10F1B-5-1 is CGMCC NO.1. 16698. The bacterial strain 10F1B-5-1 can be used for preparing the Schumannella. The Schumann bacterium 10F1B-5-1 provided by the invention has the effect of resisting gram-positive bacteria (staphylococcus aureus and bacillus subtilis) and gram-negative bacteria (acinetobacter baumannii), provides a strain resource for research and development of new antibiotics, and has better research value and application potential.

This invention discloses an Acinetobacter DL27 strain and its application in the degradation of acetaminophen, belonging to the field of microbial technology. This strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38039, deposited on April 3, 2026, and taxonomically named Acinetobacter. Acinetobacter sp . This invention has screened a strain of Acinetobacter DL27 that can efficiently degrade acetaminophen, and it can efficiently degrade acetaminophen in wastewater from actual sewage treatment plants and agricultural soils, which has important application value in the bioremediation of acetaminophen in complex environments.

The application discloses a kind of antibacterial activity of bung flower extract and preparation method thereof, the bung flower chemical component is in-depth studied in the application, and the best complex preparation of bung flower volatile oil and bung flower total flavone with best antibacterial activity is screened out, and the emulsion with good antibacterial effect and good stability with bung flower volatile oil and bung flower total flavone as active ingredient is researched.The antibacterial screening result of the application shows that bung flower total flavone and bung flower volatile oil are compounded according to specific weight-volume ratio, and have synergistic effect on staphylococcus aureus, methicillin-resistant staphylococcus aureus, pseudomonas aeruginosa, acinetobacter baumannii and escherichia coli, and good unexpected technical effect is obtained.

The invention belongs to the technical field of biological medicines, and particularly discloses a Pi scorpion polypeptide and application thereof. Through molecular biology, site-specific mutagenesis, chemical synthesis and other methods, the obtained Pi scorpion polypeptide can effectively inhibit the growth of escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, propionibacterium acnes and related drug-resistant bacteria at low concentration. The pemai Pi scorpion polypeptide has a very good effect in treating or preventing folliculitis, furuncle, impetigo, acne and whelk caused by escherichia coli, pseudomonas aeruginosa, acinetobacter baumannii, propionibacterium acnes, staphylococcus aureus, related drug-resistant bacteria and the like. The pemai Pi scorpion polypeptide disclosed by the invention has good activity in resisting escherichia coli, pseudomonas aeruginosa, acinetobacter baumannii, propionibacterium acnes, staphylococcus aureus and related drug-resistant bacteria, and can be further developed and utilized as an antibacterial drug for treating folliculitis, furuncle, impetigo, acne, whelk and the like.

The invention belongs to the technical field of biochemical engineering, and particularly relates to a P450 fusion protein, the structure of the fusion protein is VdhM3-Linker1-AciC-Linker2-AciB, VdhM3 is Vdh-M3 (I144R / N173M / Q310R), and VdhM3 is Vdh-M3 (I144R / N173M / Q310R); the GenBank login numbers of the coding genes of the AciC and the AciB are both AB221118.1, and the GenBank login numbers of the coding genes of the AciC and the AciB The amino acid sequence of the Linker2 is GSGSGH; and the Linker 1 is a flexible connector, a rigid connector or a flexible-rigid combined connector. By means of system screening, it is determined that the AciC / AciB redox partner system derived from acinetobacter has the optimal suitability with VdhM3 P450 enzyme, the catalytic activity is high, the selectivity of the target product calcifediol reaches up to 95%, generation of by-products is reduced, and the purification efficiency and yield of the product are improved.

The invention particularly discloses a screening method of microorganisms capable of relieving odor of kitchen waste. The method comprises the following steps: acquiring a sample for enrichment culture; separating and purifying on a specific selective culture medium which respectively takes degradation of amines, hydrogen sulfide and skatole as targets to obtain a single colony; and finally, extracting genome DNA of the strain, amplifying the 16S rRNA gene of the strain, and carrying out sequence alignment, so that the strain is accurately identified. By designing and applying the selective culture medium aiming at the specific odor-causing substances, efficient and directional screening of the target functional strains is realized. According to the method disclosed by the invention, the acinetobacter wuelsii, the acinetobacter baumannii and the bacillus licheniformis are successfully screened and identified, and the strains can be used for effectively degrading main odor-causing substances in the kitchen waste and have a good application prospect.

This invention relates to the pharmaceutical field, and provides a β-lactamase inhibitor or its application. The β-lactamase inhibitor is a compound of formula (I), or an optical isomer thereof, or a pharmacodynamically acceptable salt. The β-lactamase inhibitor of this invention has good water solubility, superior pharmacokinetic properties, and good drug-likeness. It has good inhibitory activity against β-lactamases of types A, B, C, and D, and can produce strong antibacterial activity when used in combination with antibiotics, especially against carbapenem-resistant Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and other bacteria, and is used to treat diseases related to bacterial infections.

The present application relates to the technical field of microfluidics, and particularly relates to a kit for rapidly detecting 15 respiratory pathogens and a use method thereof.The kit comprises a plurality of reaction cavities, each of which has a primer group corresponding to 15 respiratory pathogens; the primer group comprises inner primers, outer primers and loop primers for PCR amplification of Mycoplasma pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Stenotrophomonas maltophilia, Haemophilus influenzae, Streptococcus pneumoniae, Escherichia coli, Legionella pneumophila, Burkholderia, Staphylococcus aureus, Corynebacterium striatum, Aspergillus, Pneumocystis jirovecii and Candida albicans.The present application can simultaneously detect 15 respiratory pathogens by adding samples once, avoids adding liquid reagents to multiple detection tubes, reduces the intensity of manual operation and the risk of mismatch.

The present application aims to provide a eucheuma extract, a preparation method and application thereof, and the preparation method comprises the following steps: cutting eucheuma whole grass, using distilled water and 75% ethanol as extraction solvents respectively, hot reflux extraction twice, combining the filtrates, concentrating under reduced pressure, and evaporating the solvent to dryness to prepare a dry paste, and obtaining eucheuma water extract and eucheuma alcohol extract respectively. The eucheuma water extract and alcohol extract provided by the present application have significant inhibitory effects on staphylococcus aureus, escherichia coli, staphylococcus epidermidis, methicillin-resistant staphylococcus epidermidis, sarcina, bacillus, pseudomonas aeruginosa, enterococcus faecium, micrococcus luteus, acinetobacter baumannii, streptococcus pneumoniae and klebsiella pneumoniae, and have great development potential in the future research on bacteriostatic drugs, bacteriostatic cleaning products and other bacteriostatic products.

The application discloses a lactobacillus rhamnosus A21174 for inhibiting infection of multi-drug resistant strains and clostridium difficile, and application thereof. The lactobacillus rhamnosus A21174 has obvious inhibiting effect on drug-resistant staphylococcus aureus, pseudomonas aeruginosa, drug-resistant escherichia coli, drug-resistant acinetobacter baumannii and a model strain of clostridium difficile. Compared with a commercial strain of lactobacillus rhamnosus, the lactobacillus rhamnosus A21174 has better bacteriostatic effect on clostridium difficile. The lactobacillus rhamnosus A21174 also shows good effect in in-vivo and in-vitro experiments such as resistance to artificial gastric and intestinal fluid, resistance to bile salt, anti-aging, anti-oxidation, improvement of cognition and the like, and therefore, the strain can be used as a probiotic agent to prepare a product for regulating intestinal flora, and has important significance for preventing and / or treating diseases caused by infection of multi-drug resistant bacteria, clostridium difficile and pseudomonas aeruginosa.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide and application thereof. The antibacterial peptide provided by the invention has a remarkable inhibition effect on gram-positive bacteria such as staphylococcus aureus and enterococcus faecalis and gram-negative bacteria such as escherichia coli and acinetobacter baumannii, and the MIC value of the antibacterial peptide is 4-32 mu g / mL. Safety experiments show that under the concentration of 300 g / mL, the hemolysis rate of the antibacterial peptide is smaller than 5%; the concentration of AMP1-AMP3 is sequentially lower than 100 g / mL, 800 g / mL and 100 g / mL, the survival rate of human corneal epithelial cells is larger than 85%, toxic and side effects are small, and biocompatibility is good. Stability experiments show that the antibacterial peptide has good pH stability, thermal stability, ultraviolet irradiation stability and protease hydrolysis resistance. The antibacterial peptide provided by the invention is short in amino acid sequence, relatively low in synthesis cost, large in yield, relatively good in repeatability, high in stability and low in toxicity, and shows broad-spectrum antibacterial activity.