204 results about "Acinetobacter baumannii" patented technology

The invention discloses a method for deleting drug resistant genes of acinetobacter baumannii (AB) through CRISPR-Cas9. The method comprises the steps that firstly, AB is collected clinically, and drug resistance analysis and statistics are conducted after the AB is subjected to drug resistance measurement through a drug sensitive slip method; secondly, the multi-drug resistant AB is subjected to DNA extraction through a lysis-boiling method, and then amplification analysis is conducted by mean of well designed drug resistant gene primers of the AB; and thirdly, according to drug resistant gene detection in the former step, the AB containing an OXA-23 gene is selected, CRISPR/Cas9 and sgRNA plasmids are established and transferred into the AB containing the OXA-23 gene, an OXA-23 gene deletion AB mutant strain is established, and drug resistance analysis is conducted on the OXA-23 gene deletion AB mutant strain. The method is easy to operate and high in knocking-out efficiency, and a novel method and a novel concept are provided for preventing spreading of the drug resistant genes and treating drug resistant bacteria.

The invention provides a method for synergistically degrading oil producing wastewater by using petroleum degrading bacteria. The method comprises the following steps: (1) inoculating purified single pseudomonas aeruginosa, bacillus subtilis, pseudomonas mendocina and acinetobacter baumannii strains into a beef extract-peptone medium, and culturing for 18 hours so as to obtain seed bacterium liquid; (2) preparing an acclimation culture solution of the seed bacterium liquid; (3) adding the prepared acclimation culture solution to a fermentation medium according to a single strain bacterium liquid isopyknic ratio, and culturing; (4) putting fermented bacterium liquid in an aeration tank so as to treat the oil producing wastewater. The composite high-efficiency oil removal bacterial strain can be used for degrading more than 94% of hydrocarbon compounds in the oil producing wastewater; the selected strains are respectively high in crude oil degradation rate and have synergistic effect; the combined degradation efficiency of several bacteria for crude oil is considerably higher than the simple superimposed effect of the single effect of the bacteria.

The invention belongs to the field of microorganisms and discloses a biological agent for treating ammonia-nitrogen-containing wastewater. The biological agent comprises rhodococcus ruber, micrococcus luteus, enterococcus faecalis, acinetobacter baumannii, arthrobacter crystallopoietes, thiobacillus denitrificans and pseudomonas fluorescens. The biological agent comprises multiple strains, has the advantages of reasonable compatibility and strong synergic effect and can be used for effectively removing ammonia nitrogen, sulfide, aniline substances and industrial COD in wastewater.

The invention discloses bacteria capable of synchronously removing organic substances and ammonia nitrogen in water of a slightly polluted water source under low-temperature and aerobic conditions and a screening and domesticating method, which relate to bacteria and a screening and domesticating method and solve the problem that the prior bacteria are unsuitable for treating the slightly polluted water source as well as low temperature treatment. The bacteria SRA10 of the invention are acinetobacter lwoffi which belong to acinetobacter baumannii and are preserved in China General Microbiological Culture Collection Center on January 19th, 2009, with a preservation number of CGMCC No.2889. The screening method comprises the following steps: I, separating and purifying; II, preparing bacterial solution; III, screening and cultivating a bacterial solution; IV, screening and cultivating a bacterial solution with a live strain; V, repeating the step IV for 1 to 2 times, and stepwise domesticating the live strain; and VI, taking a bacterial solution of the domesticated strain, and rescreening the bacterial solution to obtain the bacteria. The bacteria obtained by screening and domesticating can process water of the slightly polluted water source under a condition of a low temperature of 2 to 10 DEG C.

The invention relates to recombinant protein of A1S_1523 as well as a preparation method and application of the recombinant protein. The recombinant protein comprises A1S_1523 mature peptide, and an amino acid sequence of the recombinant protein is shown in SEQ ID NO. 3. The recombinant protein disclosed by the invention is high in expression quantity and convenient to separate and purify, is efficient and safe, can be directly matched with adjuvants for use, and can be used for preparing acinetobacter baumannii infection resistant subunit vaccines and related detection kits; proven by animal experiments, gene engineering recombinant monovalent subunit vaccines have good immune protective effects on acinetobacter baumannii infection resistance; and the recombinant protein can be used for laying a foundation for further researching combined vaccines and multi-subunit fusion vaccines, and can also play important roles in the development and application of prevention and treatment vaccines and diagnostic kits.

The invention provides a gene chip for detecting pathogens of lower respiratory tract, which comprises a solid phase carrier and an oligonucleotide probe fixed on the solid phase carrier, wherein the oligonucleotide probe comprises a sequence selected from one or more DNA sequences of staphylococcus aureus, 16s-23s rDNA intergenic spacer region of Klebsiella pneumoniae or Acinetobacter baumannii, 16S gene of pseudomonas aeruginosa or haemophilus influenzae, gyrB gene of streptococcus pneumoniae or legionella pneumophila and copB gene of moraxella catarrhalis or the complementary DNA or RNA sequence. The invention further provides a reagent kit containing the gene chip. The utilization of the gene chip and the reagent kit can detect the pathogens of the lower respiratory tract; furthermore, the operation is simple, the accuracy is high and the repeatability is strong.

The present invention provides a disinfectant composition including a phage of Acinetobacter baumannii and a carrier. The present invention also provides a method for disinfecting a medical institute or a medical research institute, including the steps of applying an effective amount of a phage of Acinetobacter baumannii to the medical institute or the medical research institute for reducing amount of Acinetobacter baumannii in the medical institute or the medical research institute.

The present invention relates to gene chip for detecting several kinds of common bacterial pathogens, and the gene chip includes solid carrier and oligonucleotide probe fixed on the carrier. The chip has up to 200 low density distributed sample points. The oligonucleotide detecting probe includes DNA' s selected from nucleotide sequences corresponding to the genomes of nine kinds of common bacterial pathogens, including Staphylococcus aureus, Staphylococcus epidermidis, bacillus pyocyaneus, colibacillus, etc. The said chip together with sample treating reagent, hybridizing reagent, color reagent and the specification constitutes the detection kit. The present invention has high detection efficiency.

Disclosed are antimicrobial peptides with useful or superior properties such as antimicrobial activity, desirable levels of hemolysis, and advantageous therapeutic index against various microorganisms, especially Pseudomonas aeruginosa, Acinetobacter baumannii and Staphylococcus aureus. Also provided are methods of to control microbial growth and pharmaceutical compositions to treat or prevent microbial infections. Certain peptides are disclosed utilizing a structure-based rational modification of antimicrobial peptide D1, with single D-/L-amino acid substitutions or charged residue substitutions in or near the center of the peptide on the nonpolar or polar face, or peptides with one or more amino acids in the D configuration, and peptides with all amino acids in the D configuration. Modified peptide analogs herein can demonstrate one or more properties such as improved antimicrobial activity, specificity, and resistance to degradation. Compositions disclosed herein are useful as antibiotics, including as broad spectrum antibiotics.

The invention dislcoses acinetobacter baumannii capable of efficiently degrading imazamox, which relates to acinetobacter baumannii. The invention provides an acinetobacter baumannii strain, which is capable of efficiently degrading imazamox and provides theoretical and microbial resource foundations for the environmental modification and degradation mechanism research of an imazamox weedicide. The acinetobacter baumannii capable of efficiently degrading imazamox, disclosed by the invention, is acinetobacter baumannii IB5 which is collected in the China General Microbiological Culture Collection Center (Address: No.3, Yard 1, Beichen Road West, Chaoyang District, Beijing) with the collection number CGMCC No.4728 on April 1, 2011. The acinetobacter baumannii IB5 is capable of efficiently degrading imazamox.

The present invention discloses a strain of marine Bacillus amyloliquefaciens B01, wherein the strain is preserved in the China general microbiological culture collection center on December 11, 2014, and has the preservation number of CGMCC No.10154. According to the present invention, the fermentation broth supernatant of the Bacillus amyloliquefaciens B01 can inhibit aquatic diseases caused by a variety of pathogenic bacteria such as Acinetobacter baumannii, Vibrio parahaemolyticus and the like, and the marine Bacillus amyloliquefaciens B01 has characteristics of no toxicity on human and animals, green environmental protection, aquatic pollution disease prevention and control effect, and good biological pesticide development prospects.

The present invention provides antimicrobial peptides and analogs thereof that are cytostatic or cytotoxic toward at least one gram-negative bacterium of the genus Acinetobacter including laboratory or clinical isolates of A. baumannii with low or non-detectable cytotoxicity against mammalian cells. Optionally, the antimicrobial peptides and analogs thereof are also cytotoxic or cytostatic against other unrelated bacteria such as Staphylococcus aureus.

The invention relates to the technical field of medical detection, and in particular, relates to a primer and probe combination and a kit for detecting human pathogenic bacteria. The kit comprises theprimer and probe combination for detecting pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, staphylococcus epidermidis, staphylococcus aureus, enterococcus faecium, human staphylococcus and acinetobacter baumannii. According to the kit, the primer and probe combination is adopted, multiple digital PCR is combined, the pathogenic bacteria types and the target nucleic acid copy number in the detection range can be rapidly and accurately identified, the detection method has high sensitivity, and positive detection of low-copy nucleic acid fragments of pathogenic bacteria is guaranteed; meanwhile, the variety of the pathogenic bacteria or the change of the copy number of the target nucleic acid fragment in the body of a pathogenic bacteria infected patient can be dynamically monitored, the treatment effect is assisted and evaluated in time, and reference is provided for optimization of a doctor clinical scheme.

The invention belongs to the field of examination of microorganisms, and relates to a method for quickly identifying bloodstream infection bacteria by a high-resolution fusion curve method. The method comprises the following steps of: performing smear gram staining on a blood culture positive culture flask to distinguish negative bacteria and positive bacteria; selectively amplifying different 16S fragments for the negative bacteria and the positive bacteria; and analyzing by the high-resolution fusion curve method. Common bloodstream infection pathogenic bacteria comprise staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, enterobacter cloacae, Serratia marcescens, Klebsiella oxytoca, acinetobacter lwoffii, enterobacter aerogenes, stenotrophomonas maltophilia and burkholderia cepacia. By the method, the common bloodstream infection pathogenic bacteria can be measured quickly, efficiently and accurately, so that strong evidences are provided and precious time is striven for subsequent treatment.

The invention relates to a microbial preparation for processing industrial fermentation wastewater. The microbial preparation is prepared by mixing nitrosobacterium, Trichoderma longibrachiatum Rifai, aerobic denitrifying bacterium, acinetobacter baumannii, aspergillus niger, alcaligenes faecalis, Streptomyces lateritius, Candida albicans, Paenibacillus polymyxa and an adsorbent carrier. The microbial preparation contains multiple microbes having excellent ability of degrading non-biodegradable pollutants. Through reasonable compatibility between various strains, the microbial preparation has a good degradating effect and has a wide application prospect.

The invention discloses a biological preparation for treating high-concentration ammonia nitrogen wastewater. Preparation raw materials of the biological preparation are prepared from the following components in parts by weight: 10 to 15 parts of chlorella DH2 algal liquid, 20 to 30 parts of pseudomonas hibiscus DT1 fermented bacterial liquid, 15 to 20 parts of bacillus megaterium NCT-2 fermentedbacterial liquid, 10 to 15 parts of acinetobacter baumannii AL-6 fermented bacterial liquid, 5 to 10 parts of photosynthetic bacteria fermented bacterial liquid, 1 to 3 parts of agar and 3 to 5 partsof polyvinyl alcohol. In addition, the invention also provides a preparation method of the biological preparation for treating high-concentration ammonia nitrogen wastewater. According to the biological preparation, ammonia nitrogen in the high-concentration ammonia nitrogen wastewater is efficiently removed by utilizing a synergistic effect of heterotrophic nitrifying and aerobic denitrifying bacteria and chlorella; further, a nitrification process and a denitrification process of the biological preparation are carried out in the same device; a procedure is simple; a different procedure combination does not need to be set, and not only is the treatment efficiency of the wastewater improved, but also the running cost is saved.

The invention relates to a microbial compound fungicide for treating oil base drilling cuttings as well as a preparation method and application of the microbial compound fungicide. The compound fungicide comprises pseudomonas aeruginosa with a collection number of CGMCC NO:8983 and acinetobacter baumannii with the collection number of CGMCCNO:8984. The invention further discloses the preparation method of the microbial compound fungicide and application of the compound fungicide in treatment of oil base drilling cuttings. The preparation method includes preparation methods of liquid and solid fungicides.

The invention discloses a plant pathogenic fungi antagonistic bacterium and the application thereof in the plant disease preventing and curing. The plant pathogenic fungi antagonistic bacterium is acinetobacter baumannii BEB3 CGMCC No.2244. The acinetobacter baumannii BEB3 CGMCC No.2244 of the plant pathogenic fungi antagonistic bacterium can prevent and cure plant diseases caused by banana blight germs, banana anthracnose germs, rice blast germs, rubber dactylarin wilt disease germs, mango anthracnose germs, and bamboo fusarium germs; in particular can symbiotic to banana, can exist stably in the banana body, and can prevent and cure banana anthracnose germs and banana blight germs.

The present invention relates to: coralmycin A and B, which are novel compounds exhibiting antimicrobial activity, an isomer thereof, a derivative thereof or a pharmaceutically acceptable salt thereof; a microorganism of the genus Corallococcus producing the same; and an antimicrobial use thereof.

The coralmycin A and B have very strong antimicrobial activity against antibiotic-resistant bacteria such as MRSA, QRSA, VRE, VISA, etc.; Acinetobacter baumannii, which is a multidrug-resistant microorganism; and also against gram-positive microorganisms and gram-negative microorganisms. Therefore, the present invention can be very useful for prevention, treatment and alleviation of various microbial infections, and thus can be widely applied to the medical supply, quasi-drug, food, and feed industries.

The present invention is directed to the field of phage therapy for the treatment and control of bacterial infections, in particular diabetic foot infections. More specifically, the present invention is directed to novel cocktails of bacteriophage strains F44/10, F1 25/10, F770/05, F510/08, F1 245/05, and/or variants thereof; and methods of using same in the treatment and prevention of bacterial infections, including cutaneous ulcers associated with diabetic foot infections, caused by, e.g., Staphylococcus aureus, Pseudomonas aeruginosa, and/or Acinetobacter baumannii. The cocktails are used as pharmaceutical compositions either alone or in further combination with other therapies, e.g., antibiotics, growth factors, or other standard, as well as non-standard, therapies for diabetic foot infections.

The invention aims to provide a multiple PCR detection kit and a method for identifying susceptible bacteria species for intracranial post-operation to eliminate endogenous nucleic acid contamination. The detected bacteria species are the following common bacteria: staphylococcus aureus, enterococcus faecalis, streptococcus pneumoniae, escherichia coli, bacillus pyocyaneus, klebsiella pneumoniae and acinetobacter baumannii. Meanwhile, the invention provides the method to eliminate the endogenous nucleic acid contamination in a raw material of a PCR system and exclude a false positive experimental result of a PCR caused by the endogenous nucleic acid contamination. The detecting method using the kit has the advantages of that the operation is simple and fast, the detection cost is lowered, the detection result is accurate, and the false positive result is reduced. A negative control and a positive control are added in the amplification step, so that the judgment of false positive and false negative is effectively improved, and the detection accuracy is higher.

The invention discloses acinetobacter baumannii R9 CGMCC No.3947 and application thereof to the adsorption of heavy metal ions. The acinetobacter baumannii is tolerant to five types of ions, namely, Hg<2+>, Cu<2+>, Co<2+>, Pb<2+> and Zn<2+>, wherein the tolerance concentrations to Pb<2+> and Zn<2+> are highest and are up to 2,200 mg/L and 1,500 mg/L respectively; the tolerances to Cu<2+> and Hg<2+> are up to 800 mg/L and 400 mg/L respectively; and the tolerance to Co<2+> is 300 mg/L. Through the method, lead ions are adsorbed by the growth of thalli and can be directly adsorbed by using wet thalli of which the maximum adsorption capacity is up to 39.56 mg/g. The acinetobacter has good adsorption effect in the application of the heavy metal ions and good application prospect.

The invention discloses an LAMP (Loop-mediated isothermal amplification) kit for detecting acinetobacter baumannii, special primers of the LAMP kit and an application of the LAMP kit to detection of the acinetobacter baumannii. The nucleotide sequence of the five special primers for performing LAMP detection for the acinetobacter baumannii in a sequence table is SEQ ID NO: 2(F3), SEQ ID NO: 3(B3), SEQ ID NO: 4(FIP), SEQ ID NO: 5(BIP) and SEQ ID NO: 6(LF). The acinetobacter baumannii can be rapidly, conveniently, efficiently, high-specifically and high-sensitively detected at the equal temperature, complicated instruments are omitted, and a novel technical platform for detecting the acinetobacter baumannii is provided. The LAMP kit can be used for screening and detecting the acinetobacter baumannii in livestock production units, basic medical and health units and disease prevention and control centers, has wide market prospects and high economic and social benefits and is suitable for wide-range popularization and application.