Acinetobacter baumannii capable of efficiently degrading imazamox
A technology of Acinetobacter baumannii and imazamox, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of long residual time, screening of degrading strains, less application, and influence on normal growth of subsequent crops. , to achieve the effect of efficient degradation
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specific Embodiment approach 1
[0012] Embodiment 1: The Acinetobacter baumannii that efficiently degrades the imazethapyr strain in this embodiment is Acinetobacter baumannii IB5 (Acinetobacter baumannii IB5), which is preserved in the General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee , the deposit address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, the deposit date is April 1, 2011, and the deposit number is CGMCC No.4728.
[0013] In this embodiment, Acinetobacter baumannii IB5 (Acinetobacter baumannii IB5) is a Gram-negative bacterium, rod-shaped, with a cell size of (0.3 μm-0.5 μm)×(0.8 μm-2.2 μm), no flagella, and produces a bacterial film. Cultivated on inorganic salt solid medium, the colony is round and milky white in color.
[0014] Physiological and biochemical identification of Acinetobacter baumannii IB5 (Acinetobacter baumannii IB5) was carried out referring to the eighth edition of "Berger's Bacterial Identificati...
specific Embodiment approach 2
[0017] Embodiment 2: In this embodiment, Acinetobacter baumannii IB5 (Acinetobacter baumannii IB5) is screened from soil polluted by herbicides in Nehe City, Heilongjiang Province, China. The screening was carried out according to the following steps: Take 5g of farmland soil damaged by long-term application of imazamox in Nehe City, Heilongjiang Province, into a sterilized jar, add 50mL of normal saline, shake for 30s, filter with 8 layers of gauze, and take the filtrate 1mL was added to the inorganic salt medium, and cultured at 30°C and 180r / min for 7-10 days. During this period, imazethapyr was continuously supplemented to increase the concentration of imazamox to 800mg / L, and the enriched Culture fluid; Inorganic salt solid medium (1000mL inorganic salt solid medium is made of 1.0g of NaCl, 1.0g of NH 4 NO 3 , 1.5g of K 2 HPO 4 , 0.5g of KH 2 PO 4 , 0.1g of MgSO 4 ·7H 2 (0, 20g of agar and the remaining amount of distilled water) are sterilized and cooled to 50-60°...
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