Acinetobacter baumannii capable of efficiently degrading imazamox

A technology of Acinetobacter baumannii and imazamox, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of long residual time, screening of degrading strains, less application, and influence on normal growth of subsequent crops. , to achieve the effect of efficient degradation

Active Publication Date: 2011-11-23
海林市中农国泰生物科技有限公司
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Problems solved by technology

Because this product has a longer duration than other herbicides, it can control weeds for 4 to 6 months, so it has been widely used in North America, Europe, Southeast Asia and other countries in the early 1990s. A herbicide that is used in large quantities at home and abroad, but this t

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  • Acinetobacter baumannii capable of efficiently degrading imazamox
  • Acinetobacter baumannii capable of efficiently degrading imazamox
  • Acinetobacter baumannii capable of efficiently degrading imazamox

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specific Embodiment approach 1

[0012] Embodiment 1: The Acinetobacter baumannii that efficiently degrades the imazethapyr strain in this embodiment is Acinetobacter baumannii IB5 (Acinetobacter baumannii IB5), which is preserved in the General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee , the deposit address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, the deposit date is April 1, 2011, and the deposit number is CGMCC No.4728.

[0013] In this embodiment, Acinetobacter baumannii IB5 (Acinetobacter baumannii IB5) is a Gram-negative bacterium, rod-shaped, with a cell size of (0.3 μm-0.5 μm)×(0.8 μm-2.2 μm), no flagella, and produces a bacterial film. Cultivated on inorganic salt solid medium, the colony is round and milky white in color.

[0014] Physiological and biochemical identification of Acinetobacter baumannii IB5 (Acinetobacter baumannii IB5) was carried out referring to the eighth edition of "Berger's Bacterial Identificati...

specific Embodiment approach 2

[0017] Embodiment 2: In this embodiment, Acinetobacter baumannii IB5 (Acinetobacter baumannii IB5) is screened from soil polluted by herbicides in Nehe City, Heilongjiang Province, China. The screening was carried out according to the following steps: Take 5g of farmland soil damaged by long-term application of imazamox in Nehe City, Heilongjiang Province, into a sterilized jar, add 50mL of normal saline, shake for 30s, filter with 8 layers of gauze, and take the filtrate 1mL was added to the inorganic salt medium, and cultured at 30°C and 180r / min for 7-10 days. During this period, imazethapyr was continuously supplemented to increase the concentration of imazamox to 800mg / L, and the enriched Culture fluid; Inorganic salt solid medium (1000mL inorganic salt solid medium is made of 1.0g of NaCl, 1.0g of NH 4 NO 3 , 1.5g of K 2 HPO 4 , 0.5g of KH 2 PO 4 , 0.1g of MgSO 4 ·7H 2 (0, 20g of agar and the remaining amount of distilled water) are sterilized and cooled to 50-60°...

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Abstract

The invention dislcoses acinetobacter baumannii capable of efficiently degrading imazamox, which relates to acinetobacter baumannii. The invention provides an acinetobacter baumannii strain, which is capable of efficiently degrading imazamox and provides theoretical and microbial resource foundations for the environmental modification and degradation mechanism research of an imazamox weedicide. The acinetobacter baumannii capable of efficiently degrading imazamox, disclosed by the invention, is acinetobacter baumannii IB5 which is collected in the China General Microbiological Culture Collection Center (Address: No.3, Yard 1, Beichen Road West, Chaoyang District, Beijing) with the collection number CGMCC No.4728 on April 1, 2011. The acinetobacter baumannii IB5 is capable of efficiently degrading imazamox.

Description

technical field [0001] The invention relates to a strain of Acinetobacter baumannii. Background technique [0002] Imazamox (Imazamox) is a special herbicide of imidazolinone soybean field developed by German BASF company (formerly American cyanamide company) in the early 1980s. Grass weeds and a variety of shrubs and deciduous trees are active. Because this product has a longer duration than other herbicides, it can control weeds for 4 to 6 months, so it has been widely used in North America, Europe, Southeast Asia and other countries in the early 1990s. It is a type of herbicide that is used in a large amount at home and abroad, but this kind of herbicide remains in the soil for a long time, and it is easy to affect the normal growth of subsequent crops. [0003] At present, the research reports on imazethapyr at home and abroad mainly focus on the use and injury, but less about the screening and application of degrading strains. Contents of the invention [0004] The...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/28
Inventor 杨峰山杨鑫刘春光刘丽萍张爽黄英泽
Owner 海林市中农国泰生物科技有限公司
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