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Novel antimicrobial compound and use thereof

Inactive Publication Date: 2017-07-20
KOREA RES INST OF BIOSCI & BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent describes a new type of microorganism that produces two new compounds, coralmycins A and B, which have strong antimicrobial properties against a wide range of microorganisms, including those that are resistant to other antibiotics. This makes it useful for preventing, treating, and alleviating various microbial infections in areas like medicine, food, and feed industries.

Problems solved by technology

As such, to cope with essential life-threatening problems, it has been a major issue to discover and develop a novel antibiotic material.
Since the first global report of the vancomycin-resistant Staphylococcus aureus (VRSA), which shows a high level of resistance to vancomycin that had been referred to as the last therapeutic agent for Staphylococcus aureus (the most frequent pathogen of human infections), by the US Centers for Disease Control (CDC) in 2002, there is a growing risk of diffusion of so-called superbacteria.
In particular, no specific treatments have been developed for multidrug-resistant gram-negative bacteria and thus there is an urgent need for the development of novel anti-gram-negative antibiotics.

Method used

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  • Novel antimicrobial compound and use thereof
  • Novel antimicrobial compound and use thereof
  • Novel antimicrobial compound and use thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

Isolation and Identification of Microorganisms

[0107]A Corallococcus coralloides M23 microorganism with Accession No. of KCTC18279P was isolated from a soil sample collected from the brook located at Imhakdong-ro, Incheon, Korea according to the method reported by Park et al. (S. Park, B. Lee, J. Kim, C. Lee, E. Jang and K. Cho, Kor. J. Microbiol. Biotechnol. 32: 218, 2004).

[0108]For the identification of the Corallococcus coralloides M23 microorganism, physiological and morphological tests were performed. As a result, it was confirmed that the M23 microorganism is a gram-negative Bacillus with a length of about 4 (FIG. 1a), which showed vegetative growth feeding on E. coli, and migrated with gliding motility. Additionally, the microorganism formed its unique fruiting bodies in WC medium (10 mM 3-[N-morpholino]propanesulfonic acid (pH 7.6), 0.1% CaCl2.2H2O, 1.5% agar) (Fig. 1b).

[0109]In order to perform the identification of microorganisms based on the nucleotide sequence of 16S rDNA...

example 2

Cultivation of Microorganisms

[0110]The surface of a piece of a filter paper, on which the spores of the Corallococcus coralloides M23 microorganism were present, was spread on the entire surface of VY / 3 medium (0.5% Baker's yeast, 0.1% CaCl2.2H2O, 10 mM MOPS (pH 7.6), 1.5% bacto-agar, 0.5 ppm cyanocobalamine). After wrapping, the medium was cultured for about 10 days until the formation of fruiting bodies was confirmed at 28° C.

[0111]CYS medium (0.5% casitone, 0.1% yeast extract, 0.3% soluble starch, 0.1% MgSO4.7H2O, 0.05% CaCl2, 50 mM HEPES (pH 7.6)) in an amount of 100 mL was added to a 500 mL Erlenmeyer flask, sterilized, and then an ST-trace element solution (0.01% MnCl2.4H2O, 0.004 CoCl2.6H2O, 0.0016% CuSO4.5H2O, 0.001% Na2MoO4.2H2O, 0.002% ZnCl2, 0.0005% LiCl, 0.0005% SnCl2.2H2O, 0.001% H3BO3, 0.002% KBr, 0.002% KI, 0.08% EDTA Na—Fe3+ salt (trihydrate)) and a vitamin B12 solution (0.05% cyanocobalamine) in an amount of 100 mL, respectively, were added to the sterilized CYS med...

example 3

Isolation and Purification of Coralmycins

[0113]After the cultivation in Example 2, the amberlite XAD16 in the culture solution was recovered. Acetone was added to the amberlite XAD16 and the mixture was stirred to extract active ingredients from the amberlite XAD16. After evaporating acetone, the extract was extracted 3 times by solvent extraction using ethyl acetate. The thus-obtained ethyl acetate solvent layer containing active ingredients was concentrated under reduced pressure to remove ethyl acetate and the resulting residue was subjected to Sephadex LH-20 column chromatography using methanol as a solvent. The thus-obtained active fraction was concentrated under reduced pressure and then subjected to HPLC, in a condition where methanol:water is 50:50, to obtain compounds 1 and 2 of the active fraction.

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Abstract

The present invention relates to: coralmycin A and B, which are novel compounds exhibiting antimicrobial activity, an isomer thereof, a derivative thereof or a pharmaceutically acceptable salt thereof; a microorganism of the genus Corallococcus producing the same; and an antimicrobial use thereof.The coralmycin A and B have very strong antimicrobial activity against antibiotic-resistant bacteria such as MRSA, QRSA, VRE, VISA, etc.; Acinetobacter baumannii, which is a multidrug-resistant microorganism; and also against gram-positive microorganisms and gram-negative microorganisms. Therefore, the present invention can be very useful for prevention, treatment and alleviation of various microbial infections, and thus can be widely applied to the medical supply, quasi-drug, food, and feed industries.

Description

TECHNICAL FIELD[0001]The present invention relates to an antimicrobial compound and a microorganism having an antimicrobial activity, and more specifically, to a novel compound having an antimicrobial activity, an isomer thereof, a derivative, or pharmaceutically acceptable salt thereof; a microorganism of the genus Corallococcus producing the same; an antimicrobial composition containing the same; a pharmaceutical composition for preventing or treating microbial infections containing the composition; an antimicrobial quasi-drug composition; an antimicrobial food composition; an antimicrobial feed composition for livestock or fish; and a method for preparing the compound.BACKGROUND ART[0002]With the frequent use of antibiotics, the incidence of resistance to antibiotics has also increased. As such, to cope with essential life-threatening problems, it has been a major issue to discover and develop a novel antibiotic material. Since the first global report of the vancomycin-resistant ...

Claims

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Application Information

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IPC IPC(8): C07C233/75A61K45/06A61K31/135A23L33/00
CPCC07C233/75A61K45/06A61K31/135C07C235/56C07C235/64A61K31/166A61K35/66C07C237/42Y02A50/30
Inventor KIM, WON GONKIM, HYUN JUKIM, YU JINCHO, KYUNG YUN
Owner KOREA RES INST OF BIOSCI & BIOTECH
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