4 results about "MOPS" patented technology

The invention relates to a preserving fluid for stabilizing viruses at normal temperature and high temperature as well as a preparation method and application of the preserving fluid. The preserving fluid is an aqueous solution containing the following components in concentration: 2mM-15mM of ethylenediamine tetraacetic acid tetrasodium salt, 5mM-20mM of sodium citrate, 0.1 wt%-10wt% of tween-20, 10wt%-30wt% of a structure stabilizer, 0.5 wt%-10wt% of an amide solution, 0.5 wt%-10wt% of a hemolysis inhibitor, 0.05 wt%-10wt% of a reducing agent and 0.1 mM-2mM of 3-morpholine propanesulfonic acid. According to the preservation solution, the formula is optimized, and particularly, the amide solution and the reducing agent component are matched with MOPS for use, so that the activity of viruses in a sample under normal-temperature and high-temperature conditions can be greatly maintained, and the stability of nucleic acid is effectively improved.

The invention discloses a thromboelastogram blood coagulation activation detection reagent as well as a preparation method and application thereof. The thromboelastogram blood coagulation activation detection reagent comprises an endogenous blood coagulation activator and a composite solvent, the endogenous blood coagulation activator is selected from any one or more of ellagic acid, an ellagic acid hydrate, ellagic acid salt and a metal complex of the ellagic acid; the composite solvent comprises an organic solvent and an aqueous solvent, and the organic solvent comprises dihydrolevoglucose ketone (Cyreene) and one or more of gamma-valerolactone (GVL) and N, N-diethyl formamide (DEF); the aqueous solvent is selected from one of water, normal saline and a buffer solution, and the buffer solution is selected from any one or more of HEPES, TAPS, MOPS, BES, TES and Tris buffer solutions.

This invention discloses a reagent for determining choline in serum and its usage method, relating to the field of in vitro diagnostic reagent technology. It comprises the following components: MOPS, polyether F-68, dimethyl sulfoxide, prolin 300, potassium chloride, lithium acetate, calcium acetate, sodium tetraborate, sodium chloride, and choline. This invention provides an electrode filling solution that can be directly used to measure serum choline content using an electrode method. This method requires no sample preparation, has a short detection time, and provides stable and reliable results, avoiding the instability of choline in serum. It is compatible with appropriate equipment for quantitative detection. It solves the problem of inaccurate results due to the easy decomposition of choline during storage and also provides a low-cost method for detecting choline with results equivalent to tandem mass spectrometry.

This invention discloses a method for alleviating bioclogging through the synergistic degradation of extracellular polymers by multiple enzymes, comprising: selecting freeze-dried *Pseudomonas aeruginosa* and reviving and culturing it to obtain bacterial solution a; expanding the reviving bacterial solution a and mixing it to obtain bacterial solution b; re-inoculating bacterial solution b into a new culture medium until the logarithmic growth phase to obtain bacterial solution c; centrifuging bacterial solution c in the logarithmic growth phase and adding MOPS buffer solution to the precipitate to obtain a bacterial suspension; preparing a compound enzyme agent and adding the prepared compound enzyme agent to the bacterial suspension to react and decompose the extracellular polymers secreted by the microorganisms, thereby solving the clogging problem. This invention can alleviate the formation of bioclogging without affecting the water quality of reinjected water. Compared with single enzymes or combinations of two enzymes, it has advantages such as lower dosage, lower cost, better effect, and faster results.