36 results about "Hippuric acid" patented technology

This invention provides methods and devices for spatially separating at least first and second components in a sample which in one exemplary embodiment comprises introducing the first and second components into a first microfluidic channel of a microfluidic device in a carrier fluid comprising a spacer electrolyte solution and stacking the first and second components by isotachophoresis between a leading electrolyte solution and a trailing electrolyte solution, wherein the spacer electrolyte solution comprises ions which have an intermediate mobility in an electric field between the mobility of the ions present in the leading and trailing electrolyte solutions and wherein the spacer electrolyte solution comprises at least one of the following spacer ions MOPS, MES, Nonanoic acid, D-Glucuronic acid, Acetylsalicyclic acid, 4-Ethoxybenzoic acid, Glutaric acid, 3-Phenylpropionic acid, Phenoxyacetic acid, Cysteine, hippuric acid, p-hydroxyphenylacetic acid, isopropylmalonic acid, itaconic acid, citraconic acid, 3,5-dimethylbenzoic acid, 2,3-dimethylbenzoic acid, p-hydroxycinnamic acid, and 5-br-2,4-dihydroxybenzoic acid, and wherein the first component comprises a DNA-antibody conjugate and the second component comprises a complex of the DNA-antibody conjugate and an analyte.

The invention discloses a synthetic method for an isoquinoline formamide derivative of PET developer precursor, and belongs to the field of synthesis of stable isotope labeled precursor. The method is as follows: a) glycin and o-chlorobenzoyl chloride with proper molar ratio are subjected to acylation reaction under alkaline environment to obtain 2-chloro hippuric acid; b) the 2-chloro hippuric acid obtained in step a) and benzaldehyde are subjected to cyclization reaction under appropriate temperature, and proper amount of catalyst is added into the mixture for molecule rearrangement to obtain 1-(2-chlorpheyl)isoquinoline-3-methanoic acid; and c) the 1-(2-chlorpheyl) isoquinoline-3-methanoic acid reacts with halogeno reagent and then is ammonolyzed to obtain a compound as general formula (I). The synthetic method has the advantages that the synthetic method shortens synthetic route, reduces synthetic cost, is simple, convenient and practical, and has higher yield.

A method of using high efficiency chromatograph and mass spectrum unitedly to quickly screen inhibitor of angiotonin converzyme uses hippuric acid bialkyd as substrate and uses purified rabbit lung angiotonin converzyme as source of angiotonin converzyme for carrying out quick analysis on vitality of angiotonin converzyme in human blood plasma.

The invention discloses an organic fertilizer. The organic fertilizer comprises the following raw materials in parts by weight: 30-40 parts of emissions from flocks and herds, 20-30 parts of plant ash, 20-25 parts of Protein paste, 5-10 parts of dried pine needle, 10-14 parts of amino acid humic acid, 5-8 parts of hippuric acid, 3-7 parts of phosphate, 5-15 parts of dolomite, 11-13 parts of natural zeolite, 4-9 parts of medical stone, 12-15 parts of bean dregs, 6-8 parts of peanut shells, 4-9 parts of sawdust, 5-10 parts of urea, 3-5 parts of biological fungi, 2-3 parts of a strain powder, and 0.5-1 part of microelement. The organic fertilizer provided by the invention can increase production, improve soil, prevent diseases and restrain bacteria, and also can resist stress and remove hazards, the raw material cost is low, no residue can be left, organic nutrients are taken as the major part, and an appropriate amount of an inorganic fertilizer is reasonably proportioned, so that not only can full and reasonable nutrients be provided for crops, but also soil quality can be comprehensively improved, the soil fertility is increased, the manurial effect is quick and durable, fertilizer damage cannot be caused, and comprehensive and balanced nutrients can be provided for crops. In the formula, various trace elements and beneficial strains are provided, and the organic fertilizer provided by the invention not only can be taken as a good fertilizer source, but also can serve as a soil conditioner.

The invention belongs to the field of disease diagnostic kit, and more specifically relates to applications of a set of amino acid markers in preparation of an acute kidney injury (AKI) diagnostic agent or a kit. The amino acid marker is one or a composition of two selected from kynurenine, hippuric acid, and pidolic acid. The AUC values of the three amino acid markers in independent diagnosis ofacute kidney injury at acute renal injury early stage diagnosis are all larger than 0.7, the diagnosis distinguish effect is excellent. The diagnosis capacities of kynurenine and hippuric acid are better than that of clinically used renal function marker creatinine and early stage diagnosis marker NGAL; the diagnosis time of the above three amino acids on AKI is earlier than that of clinically used renal function marker urea nitrogen. The invention also provides a targeting amino acid metabonomic detection method capable of realizing quantitative analysis of a plurality of amino acids at the same time, the sensitivity is high, and the results are accurate and reliable.

A compound with anti-drug resistant bacteria activity having the following formula

(I) is disclosed. The methods of preparing the compound of formula (I) are also disclosed.

The invention provides a method for detecting methyl hippuric acid in urine. The method comprises the following steps: taking a urine sample, adding parachlorobenzoic-acid, hydroquinone and 3,4-dihydroxyphenylanaline into the urine sample, and oscillating, mixing and centrifuging; then adding a hydrochlric solution, sodium chloride and ethyl acetate into a supernatant obtained through centrifugation, and oscillating, mixing and centrifuging; then conducting rotary evaporation on a supernatant obtained through centrifugation; finally, adding residues obtained in the step 3 into distilled water, concentrating by nanofiltration, and conducting efficient liquid phase analysis on the concentrated solution. The method provided by the invention is high in sensitivity, precision and recovery rate, and excellent in stability; all methodological indexes conform to related requirements of Guide for Establishing Occupational Health Standards Part 5-Method for Detecting Chemical Substances in Biomaterials; the method provided by the invention can be used for detecting 3 isomers of methyl hippuric acid in urine of both occupational workers exposed to dimethylbenzene and non-occupational people exposed to dimethylbenzene.

The invention relates to a biomarker and a diagnostic kit for distinguishing prostatitis and prostatic cancer. The biomarker is selected from at least one of citric acid, L-pyroglutamic acid, DL-3-phenyl lactic acid, ethyl malonic acid, 3-hydroxy-3-methyl glutamic acid and hippuric acid. The kit comprises a reagent for quantitatively detecting the biomarker. The biomarker has differential expression in bodies of patients with prostatitis and prostatic cancer, and the difference has statistical significance. ROC curve analysis is further carried out, the result shows that the biomarker can serve as a specific marker for distinguishing prostatitis and prostatic cancer patients, the accuracy is high (AUC ranges from 0.7 to 0.9), especially citric acid, the AUC reaches up to 0.926, and it is indicated that the diagnosis accuracy is high. The invention provides a new biomarker for identifying prostatitis and prostatic cancer patients, and has the advantages of convenience in sampling, rapidness and high efficiency in detection and the like when being used for clinical auxiliary diagnosis.

Provided is a monoclonal antibody specific for hippuric acid which is one of the representative harmful hallucinogenic substances and is the major metabolite of toluene. In the present invention, a hippuric acid-carrier protein conjugate is prepared from hippuric acid and BSA or OVA as a carrier protein, using a coupling reagent and a cross-linker, mice are immunized by injection of the resulting conjugate, splenocytes are collected from animals and fused with myeloma cells, fused cells are cultured in HAT medium, a cell line producing a hippuric acid-directed monoclonal antibody is screened using a detection conjugate, a gene coding for variable regions of the screened monoclonal antibody is amplified by RT-PCR and then amplified by SOE-PCR using a linker DNA to thereby prepare a single chain variable fragment (scFV) which is cloned into a vector, thereby sequencing the base sequence and deducing amino acid sequences of the variable regions. The monoclonal antibody screened according to the present invention has a titer having a standard curve in the concentration range of mg/mL meeting requirements for the permissible exposure limit (PEL) of toluene, exhibits no cross-reactivity with carrier proteins, exhibits higher competitive inhibition in response to an increasing concentration of hippuric acid and exhibits no cross-reactivity with other proteins contained in the urine, and therefore can be usefully employed in a diagnostic kit for detection of hippuric acid which is capable of diagnosing toluene exposure.

The invention discloses a healthy aging diagnosis marker and application thereof. The marker is prepared from one or more of alpha-N-phenylacetyl-L-glutamine, 4-methylphenol, N6-methyladenosine, N1-methyl-2-pyridone-5-carboxamide, 3-phenyllactic acid, 5-hydroxyindoleacetic acid, dopamine, 4-hydroxyphenylpyruvic acid, propionylglycine, 4-aminohippuric acid, indoxyl sulfate, 3,4-dihydroxybutyric acid, O-acetylserine, N2,N2-dimethyl guanosine, N-ticagrelor glycine, threitol, methoxyacetic acid, indole-3-pyruvic acid, L-histidine, homoarginine and arachidonic acid. The marker has the characteristics of high accuracy and high sensitivity, and can be directly used as an index for healthy aging.

The invention belongs to the technical field of plant fertilizer processing, and provides an organic fertilizer for increasing the cotton yield and a preparation method thereof. The organic fertilizer is prepared from the following raw materials in parts by weight: 10-18 parts of natural potassium-magnesium sulfate, 2-10 parts of hippuric acid, 11-20 parts of dolomite, 10-12 parts of phosphate, 2-5 parts of sweet potato seedlings, 0.1-5 parts of a trace element composition, 2-6 parts of natural attapulgite, 13-14 parts of chicken manure, 10-20 parts of sawdust, 11-14 parts of bran hulls, 0.5-1 part of ferrous sulfate and 1.1-1.5 parts of an organic fertilizer synergist. The organic fertilizer for increasing the cotton yield provided by the invention can improve the cotton yield and quality, and has the advantages of simple components, easily available raw materials, low cost, simple operation, economical and practical properties, effectiveness and stability of iron, and the like.

The invention discloses synthesis methods of deuterine, hippuric acid-L-menthol ester (2,2-D2) and intermediates thereof, wherein the synthesis method includes the steps: (1) carrying out a reaction of glycine with a deuterium aqueous solution of alkali metal deuterium oxide under the action of pyridoxal or salts thereof, to obtain a deuterated reaction solution; and (2) recycling a part of deuterium water from the deuterated reaction solution obtained in the step (1) through reduced pressure distillation, then adding water and benzoyl chloride, carrying out an amidation reaction, and after the end of the reaction, posttreating to obtain hippuric acid (2,2-D2). The raw materials and reagents used in the synthesis method are cheap and easy to obtain, and the operation is simple; the purityand the deuterium abundance of the obtained deuterated reagents are high.