13 results about "Indoxyl" patented technology

The invention discloses a pharmaceutical composition containing a substituted indole-5-phenol derivative and an application of the pharmaceutical composition. Specifically, the present invention discloses a first pharmaceutical composition comprising a substituted indole-5-phenol derivative, the first pharmaceutical composition comprising (a) a therapeutically effective amount of a compound of formula (I), or a pharmaceutically acceptable salt thereof; and (b) one or more of 1, 2, 3, 4 or 5 high polymer materials. # imgabs0 #

A method for preparing a product compound of formula (II) includes purifying, from an extract, intermediate compound formula (I). The purification includes extracting mitragynine from a matrix using an extraction solvent to achieve an extract; basifying the extract, whereby the pH of the basified extract is in the range of 8-10; subjecting alkaloids precipitated in the extraction process to further extraction using an immiscible or partially miscible solvent; collecting an organic layer and the solvent from the further extraction to provide a residue concentrated in the intermediate compound formula (I); dissolving the residue in a reaction solvent to achieve an intermediate compound solution; and treating a mixture enriched with the intermediate compound solution with one or more reagents, whereby the treatment of the mixture results in the product compound of formula (II).

The invention belongs to the technical field of biomarker detection, and particularly relates to a method for synchronously and quantitatively detecting urotoxin p-cresol sulfate and indoxyl sulfate based on LC-MS / MS of an alternative analyte method and application. According to the method, stable isotope labeled pCS-d4 and INDS-d4 are creatively adopted as alternative analytes, pCS-d7 and INDS-13C6 are adopted as internal labels, a protein binding state target object is released through serum protein precipitation, a free state target object is obtained through an ultrafiltration method, a universal reversed-phase C18 chromatographic column is adopted for gradient elution and separation, and the detection result is accurate. An organic solvent containing ammonium acetate, formic acid aqueous solution-methanol and the like is used as a mobile phase, and a dual specific detection system for the total content and the free state content of the target object in the serum is established through a multi-reaction monitoring (MRM) mode of LC-MS / MS. The problem of accurate quantification of the nephropathy biomarker in a biological sample under the condition of no blank matrix is solved in a breakthrough manner, and key data support is provided for evaluating pathological toxicity risk of urotoxin.

Compounds comprising a cross-linking moiety and a protecting group are described herein along with their methods of use. The cross-linking moiety may comprise an indoxyl and the protecting group may comprise a sugar (e.g., a glucuronide or glucoside), phosphoester, or sulfoester group. The cross-linking moiety and protecting group may be attached to each other via an oxygen atom, sulfur atom, or linker. In some embodiments, the linker attaching the cross-linking moiety and protecting group is a self-immolative linker. A compound of the present invention may cross-link under physiological conditions and / or in vivo.

This invention discloses an N-N axial chiral phase-transfer catalyst, its synthesis method, and its applications, belonging to the field of asymmetric catalytic synthesis technology. Using chiral 2,4-pentanediol and N-Boc-indolephenol as starting materials, this invention efficiently constructs an N-N axial chiral biscarbazole cyclic ether skeleton through a five-step reaction involving Mitsunobu etherification, deBoc protection, PIDA oxidative cyclization, radical bromoxymethylation, and intramolecular quaternization. This method eliminates the need for complex chiral resolution and noble metal coupling, employs mild reaction conditions, uses readily available and inexpensive starting materials, achieves high overall yield, and yields a product with an axial chirality ee value ≥98%. The resulting catalyst possesses excellent conformational rigidity, chiral induction ability, and phase-transfer performance, exhibiting broad substrate applicability. It also possesses bisindole ring bioactivity, making it widely applicable in asymmetric catalytic synthesis in pharmaceuticals, fine chemicals, and other fields, and providing new directions for the development of antibacterial and antitumor biomaterials.

The present disclosure provides gene constructs comprising genes coding forenzymes involved in: converting tryptophan into indole; converting indole into indoxyl; and production of tryptophan from phosphoenolpyruvate and erythrose 4-phosphate. When such gene constructs are expressed in a genetically modified microorganism of the present disclosure, they enable the significant production and accumulation of tryptophan, indole and / or indoxyl within the cells, without the need to supply tryptophan from external sources. The genetically modified microorganism of the present disclosure includes one or more overexpressed genes alongwith one or more knocked-down genes such as tryptophan operon regulator, tyrosine aminotransferase, aspartate aminotransferase, tryptophan repressor protein, pykA, pykF, and combinations thereof. The genetically modified microorganism is capable of using glucose and glycerol as a carbon source to grow and produce indigo. The present disclosure also provides methods for preparing the genetically modified microorganism as well as method of producing indigo from said microorganism.

A biosynthetic method and composition for producing indigo dyes comprising indigotin and isatin. A tryptophanase (TRP) and a flavin monooxygenase (FMO) can be produced through a transgenic organism having carrier encoded tryptophanase and flavin monooxygenase. The produced tryptophanase and flavin monooxygenase convert L-tryptophan into indigo dyes in the presence of one or more bioactive additives selected from cysteine, 2-hydroxyindole, or 2-indoxyl, and the weight ratio of indigotin to isatin in the dyes is between 1:0.1 to 1:4. The dyes have a hue of X: 0.1920 to 0.2481, Y: 0.2537 to 0.2019, Z: 0.5543 to 0.5501 in the CIE XYZ color space definition. Selectable and repeatable dyeing hues are provided.

The present invention belongs to the technical field of protein-bound toxin detection. The present invention provides a method for simultaneously detecting the contents of three protein-bound toxins in a blood sample. The method of the present invention includes the steps of preparing a test sample solution, preparing a series of mixed reference substance solutions, performing high performance liquid chromatography determination on the series of mixed reference substance solutions, plotting the content standard curves of p-cresol sulfate, indoxyl sulfate, and 3-indoleacetic acid, performing high performance liquid chromatography determination on the test sample solution, and respectively calculating the contents of p-cresol sulfate, indoxyl sulfate, and 3-indoleacetic acid in the test sample solution according to the content standard curves. The method of the present invention can simultaneously detect three protein-bound toxins in a short time, with low cost, simple method, low requirements for the conditions of the detection instrument, simple sample treatment method, and can detect a large number of samples.

The present invention concerns glycosyltransferase enzyme mutants having improved half-lives and thermal stability compared to the parent enzyme UDP-glycosyltransferase (PtUGT) from the indigo producing plant Polygonum tinctorium / Persicaria tinctoria; and further provides a composition, kit, and methods employing these mutants for glycosylation of desired compounds, such as indoxyl compounds.

A method for preparing a product compound of formula (II) includes purifying, from an extract, intermediate compound formula (I). The purification includes extracting mitragynine from a matrix using an extraction solvent to achieve an extract; basifying the extract, whereby the pH of the basified extract is in the range of 8-10; subjecting alkaloids precipitated in the extraction process to further extraction using an immiscible or partially miscible solvent; collecting an organic layer and the solvent from the further extraction to provide a residue concentrated in the intermediate compound formula (I); dissolving the residue in a reaction solvent to achieve an intermediate compound solution; and treating a mixture enriched with the intermediate compound solution with one or more reagents, whereby the treatment of the mixture results in the product compound of formula (II).

A biosynthetic method and composition for producing an Indigo dyestuff including Indigotin and Indirubin. Tryptophanase (TRP) and flavin-containing monooxygenase (FMO) are generated from a genetically-modified organism having a vector coding for tryptophanase and flavin-containing monooxygenase. The conversion of L-tryptophan to Indigo dyestuff is converted by the generated TRP and FMO in the presence of one or more bioactive additives selected from the cysteine, 2-oxindole, or 2-indoxyl to form a dyestuff having a ratio of Indigotin to Indirubin by weight between 1:0.1 to 1:4. The dyestuff has a color hue defined by CIE XYZ color space with X: 0.1920 to 0.2481, Y: 0.2537 to 0.2019, Z: 0.5543 to 0.5501. Selectable and reproduceable color hues are provided.

The invention relates to the technical field of culture media, and particularly discloses a high-specificity group B streptococcus chromogenic culture medium and a preparation method thereof. The invention provides a high-specificity group B streptococcus chromogenic culture medium. The high-specificity group B streptococcus chromogenic culture medium specifically comprises the following components: 6-10 g / L of peptone, 2-6 g / L of yeast powder, 0.02-0.08 g / L of a chromogenic substrate, 13-17 g / L of agar, 0.01-0.05 g / L of a bacteriostatic agent and 3-7 g / L of an osmotic pressure regulator, the chromogenic substrate is prepared by mixing 4-chloro-3-indolyl-beta-D-galactopyranoside and resorufin-beta-D-galactopyranoside, and the chromogenic substrate is prepared by mixing the 4-chloro-3-indolyl-beta-D-galactopyranoside The bacteriostatic agent is formed by mixing polymyxin B and nalidixic acid; the osmotic pressure regulator is formed by mixing sodium chloride, choline chloride and iso-tridecanol polyoxyethylene ether. When the chromogenic culture medium provided by the invention is used for detecting and identifying group B streptococcus, the detection efficiency is improved, and the chromogenic culture medium has relatively high accuracy, specificity and sensitivity.

The instant disclosure is in the field of biosciences, more particularly towards molecular and industrial biotechnology. The present disclosure relates to recombinant methanotrophic bacteria capable of synthesizing indigo from methane comprising a gene encoding enzyme for increasing concentration of indole and a gene encoding enzyme for converting the indole to indoxyl. The present disclosure also relates to a method of developing the recombinant methanotrophic bacteria, and a method of indigo biosynthesis by the recombinant methanotrophic bacteria in presence of a methane source.