34 results about "Indoxyl" patented technology

The patent discloses novel 2,2-disubstituted 1,2-dihydro-3H-indol-3-one derivatives and their preparation thereof.

A spectrophotometric method for the determination of pyruvate dehydrogenase activity. This method uses a spectrophotometer, in the presence of substrate sodium pyruvate and cofactors magnesium chloride, thiamine pyrophosphate, using phenazine dimethyl sulfate as electron transporter, thiazole blue as electron acceptor, thiazole blue Reducted by pyruvate dehydrogenase (E1) product hydroxyethyl-pyrophosphate thiamine, the maximum absorption peak of thiazole blue changes from wavelength 400-430 nanometers to 540-640 nanometers, by measuring the wavelength 540-640 nanometers light absorption The amount of increase determines the reduction of thiazolium blue and defines the activity of the enzyme. The reagents used in this method are more economical, more stable, and more sensitive than the 2,6-dichloroindoxyl method and the potassium ferricyanide method. The influence of reagents is not conducive to the determination of E1 activity in the pyruvate dehydrogenase complex. This method is not only suitable for the determination of purified pyruvate dehydrogenation activity, but also for pyruvate dehydrogenation in the pyruvate dehydrogenase complex after mitochondria extraction. Hydrogenase activity assay.

The invention discloses a catalyst for aqueous-phase preparation of indole nitrogen arylide. The catalyst comprises oxides of copper salt or copper, ligands and surface active agents according to the molar ratio of 1:(0.5-50):(0.5-50). The invention further discloses a method utilizing the catalyst to realize aqueous-phase preparation of the indole nitrogen arylide. The method includes steps of adding the catalyst, indoles or indole derivatives, iodo-arylide and alkali into water to realize nitrogen arylation reaction; and treating after the nitrogen arylation reaction so as to obtain the indole nitrogen arylide. The catalyst for the aqueous-phase preparation of the indole nitrogen arylide is cheap and environment-friendly, is simple in composition, wide in application range and easy to use, and can be used for industrialization production. Reaction conditions required by the catalyst for preparing the indole nitrogen arylide are easy to control.

The invention discloses a synthesis method of glucoside based on an indoxyl derivative and a 2-(benzothiazol-2'-yl)phenol derivative. The general formula of a synthetic equation of the synthesis method is as shown in the description; the Ar-OH represents aromatic phenol or a ketonic compound thereof, and is used as a glycosyl receptor in a glycosylation reaction; the G-X represents halogenated sugar, and is used as a glycosyl donor in the glycosylation reaction. According to the synthesis method of the glucoside based on the indoxyl derivative and the 2-(benzothiazol-2'-yl)phenol derivative, a 1-acetylindolin-3-one derivative is selected and used as an intermediate; a novel synthesis method is researched and developed to prepare the glucoside based on the indoxyl derivative; a novel glycosidase fluorescence probe based on a BTP ((2-benzothiazol-2'-yl)phenyl) derivative is synthesized by applying the method; meanwhile, the application effects of the glucoside based on the indoxyl derivative and the glycosidase fluorescence probe are investigated. The glucoside based on the indoxyl derivative and the 2-(benzothiazol-2'-yl)phenol derivative can be used as glycosidase substrates, and is used for the positioning analytic detection and screening on the corresponding glycosidase, a microorganism using the corresponding glycosidase as a characteristic target, and the like.

The invention relates to a synthetic method for marine natural product mycoleptodiscin A, and belongs to the field of chemical synthesis. The method comprises the following steps: ketene sesquiterpene2 and 7-methoxyindole 3 are used as starting raw materials, the ketone sesquiterpene 2 and the 7-methoxyindole 3 are subjected to coupling to form sesquiterpene indole 4, the sesquiterpene indole 4 is methylated to form sesquiterpene indolol 5, the sesquiterpene indolol 5 reacts with an electron-withdrawing protecting group, protection is performed on -NH to form N-EWG protected sesquiterpene indole 6, the N-EWG protected sesquiterpene indole 6 is subjected to a cyclization reaction to form pentacyclic sesquiterpene indole 7, the pentacyclic sesquiterpene indole 7 is subjected to removal of N-EWG protection to form -NH pentacyclic sesquiterpene indole 8, the -NH pentacyclic sesquiterpene indole 8 is subjected to demethylation to form -NH pentacyclic sesquiterpene indoxyl 9, and finally the -NH pentacyclic sesquiterpene indoxyl 9 is subjected to an oxidation reaction to form the final natural product mycoleptodiscin A. The method provided by the invention has the characteristics of having few reaction steps, simple operation and good product selectivity and being suitable for industrialized production.

It is an object of this invention to provide a simple measurement method capable of detecting indoxyl sulfuric acid in a sample rapidly and at high sensitivity. By causing sulfatase and tetrazolium salt to act on indoxyl sulfuric acid in a sample to generate a formazan dye, and then calculating the generation amount of the formazan dye, indoxyl sulfuric acid in the sample can be measured more simply, more rapidly, and at higher sensitivity as compared with former methods.