33 results about "Indoxyl" patented technology

Compounds of formula (I)in which R1, R2, R3, R4 and R5 are hydrogen atoms or chromogenic substituents and X is hydroxyl, OR6 wherein R6 is selected from the group consisting of C1-C4 alkyl, or O-Me+ wherein Me+ is a cation derived from an organic or inorganic base; these compounds do not exhibit significant fluorescence but are capable of being cleaved by phosphatidyl-inositol-specific phospholipase C, an enzyme which is indicative of bacterial activity; the umbelliferyl moity resulting from such cleavage is a strong fluorogen thus providing effective test methods for various pathogenic bacteria, such as Listeria, Staphylococcus and Clostridium species. Also disclosed are plating media for detection of microorganisms that are capable of metabolic generation of a phosphatidyl inositol-specific phospholipase C (PI-PLC). The plating medium can be in a dry, liquid, or semi-liquid form, depending upon its water content, and comprise at least one compound capable of forming an aqueous gel when in contact with water; at least one nutrient capable of supporting growth of said microorganism; and at least one indicator compound of formula I and / or IV, notably 4-methylumbelliferyl myo-inositol-1-phosphate or salts thereof and 5-bromo-4-chloro-3-indoxyl myo-inositol-1-phosphate or salts thereof. PI-PLC generated by the microorganisms of interest leads to cleavage of the indicator compounds causing formation of fluorescence and / or color suitable for identification of type and count of such hygienically and pathologically important microorganisms as Listeria species.

Chromogenic 3-Indoxyl choline phosphate compounds of formula (I):wherein R is selected from the group consisting of hydrogen and C1-4 alkyl, such as methyl, ethyl, propyl and butyl while R<1>, R<2>, R<3>, and R<4 >are independently selected from the group consisting of hydrogen, halogen, cyano, nitro, carboxy, amino, amino substituted with one or two C1-4 alkyl groups, aminomethyl, hydroxy, C1-4 alkoxy, carboxyalkyl, and sulphonyl. These compounds are capable of being cleaved by lecithinase C leading to products which are calorimetrically detectable. The invention provides safe and sensitive detection of potentially pathogenic bacterial activity of such microbes as Clostridium perfringens, Bacillus cereus, Bacillus anthracis, Pseudomonas aeruginosa, Listeria monocytogenes, Heliobacter pylori, Legionella pneumophila, and others in material which may contain such activity typically including physiological samples, goods for consumption, such as food and beverages, and any other potentially infected objects or articles.

A method of detecting a phosphatidylinositol-specific phospholipase C enzyme by means of a substrate which is cleaved by said enzyme and yields a dye when the chromophoric portion of the substrate is dimerized and oxidized; the invention teaches using in such method, as a novel substrate, a 3-indoxyl-myo-inositol-1-phosphate compound of formula (I) wherein R is selected from the group consisting of hydrogen and C1-4 alkyl, while R1, R2, R3, and R4 are radicals selected from the group consisting of hydrogen, halogen, cyano, nitro, carboxy, amino, alkyl-substituted amino and sulphonyl; or of a salt of said formula I compound. The invention provides for a safe and sensitive method of detection of potentially pathogenic bacterial activity of such microbes as Bacillus cereus, B. Thuringiensis, Staphy lococcus aureus and various Listeria strains in potentially infected materials including physiological samples or consumable goods such as foods and beverages.

A card for fast testing residual pesticide belongs to the technical field of biological engineering, and comprises two layers of plastic plates, wherein one of the plastic plates is provided with a sampling hole and an observation hole, one face of the plastic plate is provided with water-absorbing paper and an enzyme bar for fixing drosophila melanogaster acetylcholine esterase, the water-absorbing paper opposites the sampling hole, the enzyme bar opposites the observation hole, and the water-absorbing paper is connected with the enzyme bar. A base sheet for fixing indoxyl acetic ester is bonded on the other plastic plate at the position corresponding to the enzyme bar, and a baffle is arranged between the base sheet and the enzyme bar. When the sample and the indoxyl acetic ester in theenzyme bar pre-react, the baffle is drawn out to make the enzyme bar and the base sheet contact. The card for fast testing residual pesticide has the advantages of wide application range, delicate testing and fast and simple operation.

The invention provides an indole hemicyanine fluorescent probe, a preparation method and application to detection of cyanide ions and particularly relates to a probe, i.e., 2-(2-(2-chloro-7-diethylaminoquinoline-3-yl)vinyl-N,3,3-trimethyl-3H-indole (QIE). The probe is prepared by taking 2-chloro-7-diethylaminoquinoline-3-formaldehyde and N,2,3,3-tetramethyl-3H-indole as raw materials and carryingout condensation. After the cyanide ions react with the QIE, a solution becomes colorless from purple when being observed by naked eyes; the solution becomes bright yellow green fluorescence from darkpurple fluorescence under the action of an ultraviolet lamp with 365 nm. The lowest detection limit for detecting the cyanide ions through an ultraviolet-visible spectrophotometry is 1.05*10<-6> mol / L and the lowest detection limit for detecting the cyanide ions through a fluorescence spectrophotometry is 4.16*10<-8> mol / L. The qualitative and quantitative detection of micro / trace cyanide ions ofsamples with different sources is realized through double channels including the ultraviolet-visible spectrophotometry and the fluorescence spectrophotometry.

The invention discloses a preparation method for continuously synthesizing indigotin, which comprises the steps that: liquid sodium metal reacts with ammonia gas at a certain temperature to produce sodium amide; the sodium amide is added to molten anhydrous alkali in a certain proportion, and then the mixture of the sodium amide and the anhydrous alkali is continuously added to a mixing reactor; anilino acetate is continuously added to the mixing reactor taking the anhydrous alkali as a solvent and the sodium amide as a condensing agent; and the anilino acetate is cyclized into hydroxyindole phenolate at certain temperature and pressure, and then the hydroxyindole phenolate is continuously oxidized to produce the indigotin. The alkali is potassium hydroxide, sodium hydroxide or a mixture of the potassium hydroxide and the sodium hydroxide. The method has the advantages of improving the reaction yield and the product quality, shortening the reaction period, reducing the generation of pollutants, reducing the equipment investment and the employee labor intensity, improving the working environment, and greatly reducing the energy consumption and the production cost.

A spectrophotometric method for the determination of pyruvate dehydrogenase activity. This method uses a spectrophotometer, in the presence of substrate sodium pyruvate and cofactors magnesium chloride, thiamine pyrophosphate, using phenazine dimethyl sulfate as electron transporter, thiazole blue as electron acceptor, thiazole blue Reducted by pyruvate dehydrogenase (E1) product hydroxyethyl-pyrophosphate thiamine, the maximum absorption peak of thiazole blue changes from wavelength 400-430 nanometers to 540-640 nanometers, by measuring the wavelength 540-640 nanometers light absorption The amount of increase determines the reduction of thiazolium blue and defines the activity of the enzyme. The reagents used in this method are more economical, more stable, and more sensitive than the 2,6-dichloroindoxyl method and the potassium ferricyanide method. The influence of reagents is not conducive to the determination of E1 activity in the pyruvate dehydrogenase complex. This method is not only suitable for the determination of purified pyruvate dehydrogenation activity, but also for pyruvate dehydrogenation in the pyruvate dehydrogenase complex after mitochondria extraction. Hydrogenase activity assay.

The invention discloses a catalyst for aqueous-phase preparation of indole nitrogen arylide. The catalyst comprises oxides of copper salt or copper, ligands and surface active agents according to the molar ratio of 1:(0.5-50):(0.5-50). The invention further discloses a method utilizing the catalyst to realize aqueous-phase preparation of the indole nitrogen arylide. The method includes steps of adding the catalyst, indoles or indole derivatives, iodo-arylide and alkali into water to realize nitrogen arylation reaction; and treating after the nitrogen arylation reaction so as to obtain the indole nitrogen arylide. The catalyst for the aqueous-phase preparation of the indole nitrogen arylide is cheap and environment-friendly, is simple in composition, wide in application range and easy to use, and can be used for industrialization production. Reaction conditions required by the catalyst for preparing the indole nitrogen arylide are easy to control.

The invention discloses a synthesis method of glucoside based on an indoxyl derivative and a 2-(benzothiazol-2'-yl)phenol derivative. The general formula of a synthetic equation of the synthesis method is as shown in the description; the Ar-OH represents aromatic phenol or a ketonic compound thereof, and is used as a glycosyl receptor in a glycosylation reaction; the G-X represents halogenated sugar, and is used as a glycosyl donor in the glycosylation reaction. According to the synthesis method of the glucoside based on the indoxyl derivative and the 2-(benzothiazol-2'-yl)phenol derivative, a 1-acetylindolin-3-one derivative is selected and used as an intermediate; a novel synthesis method is researched and developed to prepare the glucoside based on the indoxyl derivative; a novel glycosidase fluorescence probe based on a BTP ((2-benzothiazol-2'-yl)phenyl) derivative is synthesized by applying the method; meanwhile, the application effects of the glucoside based on the indoxyl derivative and the glycosidase fluorescence probe are investigated. The glucoside based on the indoxyl derivative and the 2-(benzothiazol-2'-yl)phenol derivative can be used as glycosidase substrates, and is used for the positioning analytic detection and screening on the corresponding glycosidase, a microorganism using the corresponding glycosidase as a characteristic target, and the like.

The invention relates to a 3-indoxyl salt that can be oxidized to indigo directly to dye textile materials instead of indigo and indigo white in situ, the preparation method of the indoxyl salt, and the indoxyl salt to be oxidized in situ to form The use of indigo to directly dye textile materials. The invention can simplify the repeated reduction-oxidation process of converting indoxyl salt into indigo in the traditional process into one step. This simplifies the process, saves the alkali and hydrosulfite consumed in multiple reduction-oxidation processes, saves manpower and material resources, thereby improving the utilization rate of equipment, reducing costs, and is conducive to environmental protection and market competition.

The invention relates to a synthetic method for marine natural product mycoleptodiscin A, and belongs to the field of chemical synthesis. The method comprises the following steps: ketene sesquiterpene2 and 7-methoxyindole 3 are used as starting raw materials, the ketone sesquiterpene 2 and the 7-methoxyindole 3 are subjected to coupling to form sesquiterpene indole 4, the sesquiterpene indole 4 is methylated to form sesquiterpene indolol 5, the sesquiterpene indolol 5 reacts with an electron-withdrawing protecting group, protection is performed on -NH to form N-EWG protected sesquiterpene indole 6, the N-EWG protected sesquiterpene indole 6 is subjected to a cyclization reaction to form pentacyclic sesquiterpene indole 7, the pentacyclic sesquiterpene indole 7 is subjected to removal of N-EWG protection to form -NH pentacyclic sesquiterpene indole 8, the -NH pentacyclic sesquiterpene indole 8 is subjected to demethylation to form -NH pentacyclic sesquiterpene indoxyl 9, and finally the -NH pentacyclic sesquiterpene indoxyl 9 is subjected to an oxidation reaction to form the final natural product mycoleptodiscin A. The method provided by the invention has the characteristics of having few reaction steps, simple operation and good product selectivity and being suitable for industrialized production.

The present invention relates to a new technology for direct oxidation of a 3-indoxyl salt into indigo blue in a novel efficient ultrasonic circulation type reactor by using air or oxygen-containing gas. According to the present invention, the new technology and the new equipment are combined, such that concentration of the existing oxide material can be substantially increased, yield and purity of the reaction can be ensured well, significant effects of energy saving and consumption reduction are provided, and indigo production with characteristics of large scale, continuality and automation can be achieved.

It is an object of this invention to provide a simple measurement method capable of detecting indoxyl sulfuric acid in a sample rapidly and at high sensitivity. By causing sulfatase and tetrazolium salt to act on indoxyl sulfuric acid in a sample to generate a formazan dye, and then calculating the generation amount of the formazan dye, indoxyl sulfuric acid in the sample can be measured more simply, more rapidly, and at higher sensitivity as compared with former methods.

The invention discloses an electrochemical method for simultaneously detecting indol sulfate and indole. According to the method, an unmodified screen-printed carbon electrode is taken as an electrode material, the advantages of background current reduction and detection sensitivity improvement of differential pulse voltammetry are combined, and simple, convenient and rapid simultaneous quantitative detection of indoxyl sulfate and indole is realized by adopting an electrochemical analysis technology according to a linear relationship between an electrochemical response signal and the concentration of a to-be-detected substance. The method does not depend on large-scale instruments and equipment, the unmodified silk-screen printing carbon electrode has the advantages of being easy to prepare, low in price, disposable and the like, the detection method has good repeatability and stability, and a new method is provided for studying metabolism and effects of indol sulfate and indole.