58 results about "Acetylcholine esterase" patented technology

The invention belongs to the technical field of monatomic nano-enzyme material preparation, and particularly relates to a nano-enzyme preparation method for acetylcholin esterase activity detection. The Ni-NiO heterojunction material is successfully synthesized by adopting Ni-MOF as a carrier and accurately regulating and controlling the ratio of nitrogen to air in the ultrafast Joule hot calcination process. The material has rich oxygen vacancies and metal Ni sites, the oxygen vacancies and the metal Ni sites can effectively serve as anchoring sites of Pt single atoms, the loading capacity and dispersity of the Pt single atoms are remarkably improved, and therefore the excellent nano-enzyme catalytic activity is shown. In addition, the preparation method disclosed by the invention is simple and convenient in process and has a wide industrial application prospect. An acetylcholin esterase activity colorimetric sensing method constructed on the basis of the nanometer enzyme for acetylcholin esterase activity detection prepared by the method has extremely high sensitivity and low detection limit. The detection limit of the technology can be as low as 0.037 mU / mL, the problem that the sensitivity of a traditional detection method in a low-concentration sample is insufficient is remarkably solved, and remarkable clinical application potential is shown.

The invention relates to the technical field of preparation and application of functional materials, in particular to a fullerene / metal organic framework loaded with acetylcholin esterase as well as a preparation method and application of the fullerene / metal organic framework. The preparation method comprises the following steps: firstly, preparing a fullerene / metal organic framework C60-coated PCN-222 (Fe) nano-enzyme with a space limited environment, and providing a self-cascade amplification strategy without external energy input based on the activities of oxidase-like OXD and peroxidase-like POD of the fullerene / metal organic framework C60-coated PCN-222 (Fe) nano-enzyme; then, the C60 atched PCN-222 (Fe) is combined with acetylcholin esterase AChE by means of molecular interaction, and an AChE / C60 atched PCN-222 (Fe) enzyme-nano enzyme array flow reactor is further developed. The sensor realizes parallel real-time detection and distinguishing of a plurality of organophosphorus pesticides, and has the advantages of wide linear range, high sensitivity and low detection limit. A promising strategy is provided for design of a nano-enzyme and trace pollutant detection system, and a sensing platform is stable in performance, low in price and convenient for commercialized application.

The invention discloses a preparation method of a carbon dot nano-enzyme, which comprises the following steps: dissolving oxalic acid, polyethyleneimine and ferric nitrate in ultrapure water, heating, mixing and reacting, cooling to room temperature, centrifuging, filtering, dialyzing, and taking supernate to obtain the carbon dot nano-enzyme. The prepared oxalic acid carbon dot nano-enzyme is matched with acetylcholin esterase, acetylcholine, 3, 3 ', 5, 5'-tetramethyl benzidine and H2O2 to construct a colorimetric and fluorescent dual-mode detection method for glyphosate in fruits and vegetables. The oxalic acid carbon dot nano-enzyme is prepared by doping ferric nitrate and tetraethylenepentamine into oxalic acid serving as a precursor, the oxalic acid carbon dot nano-enzyme has excellent peroxidase-like activity, a colorimetric and fluorescent dual-mode detection method of glyphosate is constructed through the oxalic acid carbon dot nano-enzyme, dual-mode detection results can be mutually verified, and the detection result is accurate. And the accuracy and the reliability of a detection result are remarkably improved.

The invention belongs to the technical field of medicines, and particularly relates to a triterpenoid extracted from edible and medicinal fungus ganoderma lucidum, a preparation method of the triterpenoid and application of the triterpenoid in the aspect of resisting Alzheimer's disease. The triterpenoid compound is shown as a structural formula I. The triterpenoid compound provided by the invention has remarkable acetylcholin esterase inhibitory activity, is an ideal candidate compound of an acetylcholin esterase inhibitor which is novel in structure and efficient in activity, and has a good application prospect in the aspect of treatment of Alzheimer's disease. # imgabs0 #

The invention discloses a paper-based multicolor colorimetric method for detecting acetylcholin esterase activity or organophosphorus pesticide, and the method comprises the following steps: depositing a nanogold bipyramid on a nylon film, and constructing a paper-based analysis device; the method comprises the following steps: adding a reaction solution containing Au < + > into a paper-based analysis device, and inducing the biconical appearance conversion of the nanogold through a disproportionation reaction of Au < + >, so that the biconical nanogold generates color change and position change of a longitudinal localized surface plasmon resonance peak lambda LLSPR; therefore, visual semi-quantitative detection and quantitative detection of acetylcholin esterase activity or organophosphorus pesticide by the ultraviolet spectrophotometry are realized. Compared with an existing method, the Au < + >-induced nanogold biconical appearance conversion reaction can effectively avoid interference of a reducing agent in an actual sample. Meanwhile, the constructed paper-based analysis method shows better nanogold bipyramid storage stability, reaction reproducibility and storability of detection results, and also has the advantages of high etching efficiency and high detection sensitivity.

The invention discloses a neuroprotective peptide with anti-inflammatory and anti-oxidation effects and application of the neuroprotective peptide. The neuroprotective polypeptide is a polypeptide with any one of the following sequences: FPDFVYK and APDTRLF. The invention provides an antioxidant effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing antioxidant drugs. The invention provides an anti-inflammatory effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing anti-inflammatory drugs. The invention provides the influence of the neuroprotective polypeptide on the expression of pathways and neurotrophic factors in PC12 nerve cells. The neuroprotective polypeptide reduces the activity of acetylcholin esterase (AChE) and up-regulates the expression of neurotrophic factors so as to alleviate nerve cell damage and enhance memory, thereby achieving the purpose of preventing and treating neurodegenerative diseases.

The invention belongs to the technical field of food nutrition, and discloses application of an artemisia selengensis extract in preparation of a medicine for improving neurodevelopmental disorder and the medicine. The artemisia selengensis extract has the following advantages and effects: the research finds that the artemisia selengensis extract has the effect of improving neurodevelopmental disorders, which is specifically embodied in that the artemisia selengensis extract can effectively increase the activity of acetylcholin esterase, lower the content of ROS (reactive oxygen species), up-regulate antioxidant indexes (GSH (glutathione) content and sod1 and cat gene expression) and relieve oxidative stress injury of zebrafish caused by NP (natriuretic peptide); meanwhile, the artemisia selengensis extract can also up-regulate the expression of a neural development gene (alpha 1-tubulin, mbp) so as to improve the neural development disorder of the zebrafish; besides, the mechanism of improving the zebrafish neurodevelopmental disorder by the artemisia selengensis extract is mainly realized through a p38 / 5-HT / Wnt / beta-catenin signal channel, expression of p38a is down-regulated (network pharmacology finds that p38 in MAPHK is a main mechanism channel), and expression of htr1aa, maoa, wnt4a and beta-catenin is down-regulated.

The invention relates to the technical field of natural medicines, and discloses huperzine A C12 site arylation series derivatives and a preparation method thereof, and application of the derivatives in preparation of medicines for treating central nervous system degenerative diseases. The derivative has a structure as shown in a general formula I. According to the preparation method, a natural product huperzine A is taken as a raw material, aryl bromide is taken as a substrate, one-step synthesis is carried out through palladium-catalyzed heck coupling reaction, and the compound has the advantages of high chemical selectivity, no need of functional group protection, rapidness and the like. In-vitro enzyme activity and cell experiments prove that the derivative not only shows potential acetylcholin esterase inhibitory activity, but also has high efficiency, low toxicity and better neuroprotective activity, and has a prospect in clinical treatment of Alzheimer's disease. The preparation of the derivatives also provides a design thought for the development of novel central nervous system disease treatment drugs.

The invention discloses a rapid screening and detecting method for vegetable pesticide residues, a multi-dimensional judgment basis is provided for pesticide residue detection by combining a PCR amplification result with the inhibition rate of acetylcholin esterase and butyrylcholin esterase, different types of pesticides may induce different gene expression changes, and the detection result is accurate. According to the rapid screening and detecting method for the pesticide residues in the vegetables, the accuracy of a detection result is improved, meanwhile, the variety of the pesticide residues in the vegetables can be accurately determined, the defect that specific pesticide varieties are difficult to distinguish by purely relying on an enzyme inhibition method is overcome, and the detection result is more targeted and accurate.

The invention discloses a quinine derivative as well as application and a preparation method thereof. The quinine derivatives are a series of multi-target guiding ligands with MAO-B and acetylcholin esterase inhibition effects at the same time, and can be used for preventing or treating related diseases, especially Alzheimer's disease and Parkinson's disease. # imgabs0 #

In alternative embodiments, provided are polyheterocyclic uncharged bisoxime antidotes, including polyheterocyclic uncharged bisoximes, and methods for treating, preventing or ameliorating excessive acetylcholine stimulation in the brain comprising administering to an individual in need thereof a polyheterocyclic uncharged bisoxime antidote as provided herein. In alternative embodiments, provided are methods for treating, preventing or ameliorating excessive acetylcholine stimulation in the brain; or, treating, ameliorating or protecting (preventing) an organophosphate toxicity or poisoning or toxic exposure, or for treating, ameliorating or protecting (preventing) organophosphate inhibition of an acetylcholinesterase (AChE) comprising administering to an individual in need thereof a polyheterocyclic uncharged bisoxime antidote as provided herein.

The application discloses a preparation method of carbon dot nanometer enzyme, which comprises the following steps: dissolving oxalic acid, polyacetyl imine and ferric nitrate in ultrapure water, heating and mixing, cooling to room temperature, centrifuging, filtering, dialyzing and taking supernatant to obtain the carbon dot nanometer enzyme; and a double-mode detection method for colorimetric and fluorescent detection of glyphosate in fruits and vegetables is constructed by using the prepared oxalic acid carbon dot nanometer enzyme, acetylcholinesterase, acetylcholine, 3,3',5,5'-tetramethyl benzidine and H2O2. The oxalic acid carbon dot nanometer enzyme is prepared by doping ferric nitrate and tetraethylenepentamine with oxalic acid as a precursor, has excellent peroxidase-like activity, and a colorimetric and fluorescent double-mode detection method for glyphosate is constructed by using the oxalic acid carbon dot nanometer enzyme, the double-mode detection results can be verified with each other, and the accuracy and reliability of the detection results are significantly improved.

The present invention provides an application of fermented Lactobacillus mucilaginosus IOB802 in improving memory, and relates to the field of microorganisms. The bacterial powder of fermented Lactobacillus mucilaginosus IOB802 and its postbiotics significantly increase the acetylcholine level of cognitive impairment mice and reduce the acetylcholinesterase level; and the serum superoxide dismutase is significantly increased, and the malondialdehyde level is significantly reduced. Therefore, the bacterial powder of fermented Lactobacillus mucilaginosus IOB802 and its postbiotics can alleviate the oxidative damage level of mice, thereby alleviating nerve damage, and have the effect of improving memory.

The invention provides a phenolic compound with a hangover alleviating effect and a preparation method thereof. The structural formula of the polyphenol compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosylphenyl) ethanone is as shown in formula (I), and the polyphenol compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosylphenyl) ethanone is separated from pueraria thomsonii. In-vitro pharmacological experiments prove that the compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosyl phenyl) ethanone has good acetylcholin esterase inhibitory activity, and the EC50 value of the compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosyl phenyl) ethanone is 0.41 + / -0.07 mM, which indicates that the compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosyl The polyphenol compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosylphenyl) ethanone is expected to be used as a lead compound to develop a novel alcohol effect dispelling and liver protecting medicine.

The invention provides a detection sensor and application thereof, and belongs to the field of pesticide detection. The detection sensor provided by the invention comprises an acetylcholine solution, an acetylcholin esterase solution, a choline oxidase solution, a Cu-BTC dispersion liquid and a solution containing a color developing agent capable of generating a hydroxyl color developing group, or comprises acetylcholine, a color developing agent capable of generating a hydroxyl color developing group and a Cu-BTC-based composite material; the Cu-BTC based composite material is prepared from Cu-BTC, choline oxidase and acetylcholin esterase, wherein the choline oxidase and the acetylcholin esterase are loaded on the Cu-BTC. Acetylcholine is hydrolyzed by acetylcholin esterase to generate choline, the choline is oxidized by choline oxidase to generate H2O2, the H2O2 is oxidized by Cu-BTC to generate. OH, and the. OH can oxidize a color developing agent to generate a colored substance. The organophosphorus pesticide can inhibit the activity of acetylcholin esterase to change the color of the solution, and then quantitative analysis of the organophosphorus pesticide is realized through the absorbance and the absorbance of a standard solution.

The invention discloses a three-component metal aerogel nano-enzyme for detecting the activity of acetylcholin esterase and a preparation method of the three-component metal aerogel nano-enzyme. The preparation method of the three-component metal aerogel nano-enzyme comprises the following steps: dissolving four metal salts in absolute ethyl alcohol according to a ratio, and ultrasonically mixing uniformly; preheating in a water bath, adding a freshly prepared sodium borohydride ethanol solution, quickly stirring, and standing to obtain three-component metal organic gel; and after multiple times of solvent exchange is conducted through acetone, supercritical carbon dioxide drying is conducted, and the three-component metal aerogel nano-enzyme is obtained. The preparation method of the three-component metal aerogel nano-enzyme has the characteristics of relatively simple process, mild reaction conditions and no surfactant; the three-component metal aerogel nano-enzyme prepared by the method has a self-supporting three-dimensional porous structure, has excellent peroxidase-like activity, and can be used for detecting the activity of acetylcholin esterase.

The invention provides a method for rapidly detecting pesticide residues in fruits and vegetables, and belongs to the field of pesticide analysis and detection. The method comprises the following steps: mixing a copper nanoparticle / reduced graphene oxide / chitosan composite material, acetylcholin esterase, choline oxidase and a cross-linking agent glutaraldehyde solution for reaction to obtain a double-enzyme functionalized nano composite material; dispensing a chitosan solution on the surface of a working electrode of a screen-printed electrode, drying to form a film, and covalently modifying the surface of the electrode with the bienzyme functionalized nano composite material through a cross-linking agent glutaraldehyde to obtain a sensing electrode; according to the method, acetylcholine is taken as a substrate, a sensing electrode is used, a chronoamperometry is adopted, inhibition ratios of pesticide standard substance solutions with different known concentrations to electrode response current are detected, and a standard curve between the pesticide concentration and the current inhibition ratio is established; and calculating the pesticide residue concentration in the fruit and vegetable sample to be detected according to the current inhibition ratio of the liquid to be detected and the standard curve. Therefore, the sensitivity and accuracy of pesticide residue detection in fruits and vegetables are improved.

The invention discloses a preparation method of an electrochemical sensor for organophosphorus pesticide residues in traditional Chinese medicinal materials based on AuNPs / BiVO4, and relates to the field of electrochemical sensors, the method comprises preparation of a BiVO4 / GCE modified electrode, preparation of an AuNPs / BiVO4 / GCE modified electrode, and preparation of an AChE / PANI / AuNPs / GCE electrochemical sensor. According to the invention, BiVO4 and AuNPs are used as electrode substrate materials to load acetylcholin esterase (AChE), so that the electrochemical enzyme biosensor which takes AChE as a recognition element and has high sensitivity, high selectivity, recognition and fast detection is constructed, and the electrochemical enzyme biosensor is used for detecting organophosphorus pesticide residues in traditional Chinese medicinal materials. The sensor detects Ops by using an enzyme inhibition principle, shows higher detection sensitivity and wider detection range, shows high stability and reproducibility in detection of organic phosphorus pesticide residues in traditional Chinese medicinal materials, and provides a more effective technical means for safety monitoring of the traditional Chinese medicinal materials.

The application provides a kind of gold nanoclusters with polypeptide as ligand and detection method of methyl parathion, the sequence of polypeptide in the gold nanoclusters with polypeptide as ligand is H (S, D) GGFFYCC;The gold nanoclusters with polypeptide as ligand can be used for double signal detection of methyl parathion and detection of methyl parathion in fruits and vegetables.Fluorescence signal detection: gold nanoclusters are added to the test solution, 37 DEG C reaction 30-60 min, detect the fluorescence change at 700 nm.Colorimetric signal: in pH=8.0 buffer solution, different concentrations of methyl parathion, acetylcholinesterase, choline oxidase and acetylcholine are added, 37 DEG C preheating 30-60 min.pH=4.0 buffer solution, gold nanoclusters and TMB are added, 37 DEG C reaction 10 min, 652 nm is measured absorbance.The detection method of the application has high detection sensitivity, strong anti-interference ability, low detection limit, high correlation coefficient of detection curve, good reproducibility in fruit and vegetable detection recovery experiment, and has good practical value in the detection of methyl parathion.

The invention provides a detection sensor and application thereof, and belongs to the field of pesticide detection. The detection sensor provided by the invention comprises acetylcholine, acetylcholin esterase, choline oxidase, two-dimensional Cu-TCPP (Fe) NSs and a color developing agent capable of generating a hydroxyl color developing group. The acetylcholin esterase can hydrolyze acetylcholine to generate choline, the choline is oxidized by the choline oxidase to generate H2O2, the H2O2 is oxidized by the two-dimensional Cu-TCPP (Fe) NSs to generate hydroxyl free radicals (. OH), and the. OH can oxidize a color developing agent capable of generating hydroxyl color developing groups to generate colored substances. When the organophosphorus pesticide exists in the to-be-detected liquid, the color of the solution is light blue or colorless; otherwise, the color of the solution is dark blue. The organophosphorus pesticide can inhibit the activity of acetylcholin esterase, so that the color of the solution is changed in the whole cascade catalytic amplification reaction, and then quantitative analysis of the organophosphorus pesticide is realized through a concentration-absorbance curve of the solution absorbance and the standard solution.

The present invention relates to a composition for preventing, alleviating, or treating cognitive dysfunction, containing a Zophobas atratus extract as an active ingredient. Specifically, when the Zophobas atratus extract of the present invention is administered to a scopolamine-induced cognitive dysfunction animal model, the cognitive function of learning ability and memory is enhanced, the expression of postsynaptic density protein-95 (PSD95), neuronal nuclear antigen (NeuN), and choline acetyltransferase (ChAT) proteins is increased, and the expression of acetylcholine esterase (AChE) proteins is reduced. Therefore, the Zophobas atratus extract of the present invention can be useful as a material for functional health foods, medicines, feed additives for companion animals, and the like for preventing, alleviating, or treating cognitive dysfunction.

The invention belongs to the technical field of natural product extraction and fermentation engineering, and particularly relates to a tobacco fermentation liquor extract with acetylcholin esterase inhibition and antioxidant activity as well as a preparation method and application of the tobacco fermentation liquor extract. Specifically, research finds that by taking tobacco leaves or tobacco waste as a substrate and screening a nicotine-resistant microbial strain with high fermentability for co-culture fermentation, acetylcholin esterase inhibition and antioxidant activity of metabolite from a single plant or microorganism source can be effectively improved; therefore, the tobacco fermentation liquor extract with acetylcholin esterase inhibition and antioxidant activity is obtained. Tobacco leaves or tobacco wastes are used as raw materials to prepare products with biomedical potential, and a new way is provided for promoting multipurpose utilization of tobaccos.

The invention discloses a composition for assisting in improving memory and application of the composition, and particularly relates to the technical field of health care products. The composition comprises broccoli leaf polypeptide, pleurotus nebrodensis polypeptide, gastrodia elata protein peptide and N-acetylneuraminic acid in a mass ratio of 1: (0.5-1.5): (2-3): (0.1-0.3). According to the invention, the four components cooperate to promote secretion of acetylcholine and reduce the content of acetylcholin esterase, so that the composition has the efficacy of assisting in improving memory and cognitive ability, and can be applied to health care products.

The application discloses a kind of compositions for assisting to improve memory and application thereof, and specifically relates to health product technical field.The composition includes broccoli leaf polypeptide, white mushroom polypeptide, gastrodin protein peptide and N-acetylneuraminic acid, and the mass ratio is 1:(0.5-1.5):(2-3):(0.1-0.3).The application promotes acetylcholine secretion by four components synergistically, reduces acetylcholinesterase content, so that the composition has the efficacy of assisting to improve memory and cognitive ability, and can be applied to health product.

The invention discloses a multi-target anti-senile dementia activity holothuria scabra-derived lipid compound as well as a separation component and application thereof, and belongs to the field of medicines and functional foods. The preparation method of the holothuria scabra lipid compound comprises the following steps: extracting holothuria scabra total lipid by using a mixed solvent of chloroform and methanol, deoiling by using cold acetone, and then separating and collecting precipitate to obtain the holothuria scabra lipid compound. The holothuria scabra separation component is obtained by carrying out silica gel column chromatography on the holothuria scabra lipid compound, and eluting by taking a mixed solution of dichloromethane, methanol and / or water as an eluent, so as to obtain a primary component with activity after elution; respectively carrying out normal-phase column chromatography on the two components with the best activity, and eluting by using a mixed solvent of ethyl acetate and normal hexane and / or a mixed solvent of dichloromethane and methanol to obtain a secondary component; the holothuria scabra lipid complex and the separated component thereof have remarkable Abeta neurotoxicity resistance, neuroinflammation resistance, intracellular oxidative stress resistance, free radical scavenging and acetylcholin esterase inhibition activity.

The invention provides application of boxthorn leaf total alkaloids in preparation of an acetylcholin esterase inhibitor, and belongs to the technical field of traditional Chinese medicines. The invention proposes that the boxthorn leaf total alkaloid has an acetylcholin esterase inhibiting effect for the first time, and the boxthorn leaf total alkaloid can be developed into an acetylcholin esterase inhibitor for preventing and treating diseases related to abnormal rise of acetylcholin esterase. Meanwhile, compared with an existing boxthorn leaf total alkaloid preparation method in the field, the boxthorn leaf total alkaloid preparation method provided by the invention is simpler, and the boxthorn leaf total alkaloid can be obtained without extracting by using organic solvents such as ethyl acetate and the like which are mutually soluble with water.

The application relates to the technical field of material science, analytical chemistry and food safety detection technology, in particular to a ratio fluorescent carbon dot microneedle patch for detecting pesticide residues of fruits and vegetables, and a preparation method thereof. The preparation method comprises the following steps: Fe-CDs and MnO2 are prepared into Fe-CDs / MnO2 nanosheet hybrid materials; the Fe-CDs / MnO2 nanosheet hybrid materials, acetylcholine, acetylcholine esterase and o-phenylenediamine are added into a PVA solution under vacuum conditions; a solvent and a crosslinking agent are further added; after the reaction is completed, the Fe-CDs / MnO2 / PVA microneedle patch is obtained by pouring into a microneedle patch mold and drying. The microneedle patch can realize rapid, accurate, on-site operable and low-cost detection of pesticide residues of fruits and vegetables in combination with RGB color recognition software.

The invention belongs to the technical field of natural medicines and pharmacology, and particularly relates to a raspberry extract as well as a preparation method and application thereof. Aiming at the problems of brain fatigue and functional hypomnesia caused by high-intensity brainwork of middle-aged and young people below 50 years old in the modern society, the invention provides a preparation process of a targeted enriched active monomer, which comprises the following steps: by taking dried raspberry fruits as a raw material, firstly, carrying out reflux degreasing with n-hexane to remove fat-soluble impurities; and sequentially extracting the degreased filter residues step by step by using ethanol with different concentrations, combining extracting solutions, and concentrating and drying to obtain the traditional Chinese medicine composition. The extract obtained by the preparation process can remarkably improve dysmnesia induced by scopolamine and inhibit the activity of acetylcholin esterase, is suitable for preparing products for relieving mental fatigue and improving learning efficiency, and does not relate to treatment of degenerative diseases such as senile dementia.

The invention provides a novel application of a Wuzi Yanzong pill, and particularly discloses a novel application of the Wuzi Yanzong pill in preparation of medicines for reducing blood sugar, improving diabetic neuropathy or preventing and treating Alzheimer's disease. Experiments prove that the Wuzi Yanzong pill has the effects of reducing blood sugar, protecting peripheral nerves, eliminating peripheral neuroinflammation, improving reaction capacity, recovering dyskinesia and inhibiting acetylcholin esterase activity, and application of the Wuzi Yanzong pill in medicines for reducing blood sugar and improving diabetic neuropathy and medicines for preventing and treating Alzheimer's disease is proved.

The invention belongs to the technical field of medicines, and relates to 19 amide alkaloids, including 5 pairs of amide alkaloid enantiomers, extracted and separated from roots of Piper nigrum L. of Piperraceae plant. A crude pepper root extract is separated through silica gel column chromatography, HP20 column chromatography, ODS (octadecylsilyl) column chromatography and HPLC (high performance liquid chromatography) separation in sequence, and finally 19 amide alkaloids including 5 pairs of amide alkaloid enantiomers are obtained. The invention also relates to investigation on the biological activity of the compounds. The preparation method is simple, the reproducibility is good, the purity of the obtained compound is high, and the obtained compound has acetylcholin esterase inhibitory activity, butyrylcholin esterase inhibitory activity and beta-amyloid protein aggregation inhibitory activity.