28 results about "Acetylcholine esterase" patented technology

The invention belongs to the technical field of monatomic nano-enzyme material preparation, and particularly relates to a nano-enzyme preparation method for acetylcholin esterase activity detection. The Ni-NiO heterojunction material is successfully synthesized by adopting Ni-MOF as a carrier and accurately regulating and controlling the ratio of nitrogen to air in the ultrafast Joule hot calcination process. The material has rich oxygen vacancies and metal Ni sites, the oxygen vacancies and the metal Ni sites can effectively serve as anchoring sites of Pt single atoms, the loading capacity and dispersity of the Pt single atoms are remarkably improved, and therefore the excellent nano-enzyme catalytic activity is shown. In addition, the preparation method disclosed by the invention is simple and convenient in process and has a wide industrial application prospect. An acetylcholin esterase activity colorimetric sensing method constructed on the basis of the nanometer enzyme for acetylcholin esterase activity detection prepared by the method has extremely high sensitivity and low detection limit. The detection limit of the technology can be as low as 0.037 mU / mL, the problem that the sensitivity of a traditional detection method in a low-concentration sample is insufficient is remarkably solved, and remarkable clinical application potential is shown.

The invention discloses a neuroprotective peptide with anti-inflammatory and anti-oxidation effects and application of the neuroprotective peptide. The neuroprotective polypeptide is a polypeptide with any one of the following sequences: FPDFVYK and APDTRLF. The invention provides an antioxidant effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing antioxidant drugs. The invention provides an anti-inflammatory effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing anti-inflammatory drugs. The invention provides the influence of the neuroprotective polypeptide on the expression of pathways and neurotrophic factors in PC12 nerve cells. The neuroprotective polypeptide reduces the activity of acetylcholin esterase (AChE) and up-regulates the expression of neurotrophic factors so as to alleviate nerve cell damage and enhance memory, thereby achieving the purpose of preventing and treating neurodegenerative diseases.

The invention discloses a rapid screening and detecting method for vegetable pesticide residues, a multi-dimensional judgment basis is provided for pesticide residue detection by combining a PCR amplification result with the inhibition rate of acetylcholin esterase and butyrylcholin esterase, different types of pesticides may induce different gene expression changes, and the detection result is accurate. According to the rapid screening and detecting method for the pesticide residues in the vegetables, the accuracy of a detection result is improved, meanwhile, the variety of the pesticide residues in the vegetables can be accurately determined, the defect that specific pesticide varieties are difficult to distinguish by purely relying on an enzyme inhibition method is overcome, and the detection result is more targeted and accurate.

In alternative embodiments, provided are polyheterocyclic uncharged bisoxime antidotes, including polyheterocyclic uncharged bisoximes, and methods for treating, preventing or ameliorating excessive acetylcholine stimulation in the brain comprising administering to an individual in need thereof a polyheterocyclic uncharged bisoxime antidote as provided herein. In alternative embodiments, provided are methods for treating, preventing or ameliorating excessive acetylcholine stimulation in the brain; or, treating, ameliorating or protecting (preventing) an organophosphate toxicity or poisoning or toxic exposure, or for treating, ameliorating or protecting (preventing) organophosphate inhibition of an acetylcholinesterase (AChE) comprising administering to an individual in need thereof a polyheterocyclic uncharged bisoxime antidote as provided herein.

The application discloses a preparation method of carbon dot nanometer enzyme, which comprises the following steps: dissolving oxalic acid, polyacetyl imine and ferric nitrate in ultrapure water, heating and mixing, cooling to room temperature, centrifuging, filtering, dialyzing and taking supernatant to obtain the carbon dot nanometer enzyme; and a double-mode detection method for colorimetric and fluorescent detection of glyphosate in fruits and vegetables is constructed by using the prepared oxalic acid carbon dot nanometer enzyme, acetylcholinesterase, acetylcholine, 3,3',5,5'-tetramethyl benzidine and H2O2. The oxalic acid carbon dot nanometer enzyme is prepared by doping ferric nitrate and tetraethylenepentamine with oxalic acid as a precursor, has excellent peroxidase-like activity, and a colorimetric and fluorescent double-mode detection method for glyphosate is constructed by using the oxalic acid carbon dot nanometer enzyme, the double-mode detection results can be verified with each other, and the accuracy and reliability of the detection results are significantly improved.

The invention provides a phenolic compound with a hangover alleviating effect and a preparation method thereof. The structural formula of the polyphenol compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosylphenyl) ethanone is as shown in formula (I), and the polyphenol compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosylphenyl) ethanone is separated from pueraria thomsonii. In-vitro pharmacological experiments prove that the compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosyl phenyl) ethanone has good acetylcholin esterase inhibitory activity, and the EC50 value of the compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosyl phenyl) ethanone is 0.41 + / -0.07 mM, which indicates that the compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosyl The polyphenol compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosylphenyl) ethanone is expected to be used as a lead compound to develop a novel alcohol effect dispelling and liver protecting medicine.

The invention discloses a three-component metal aerogel nano-enzyme for detecting the activity of acetylcholin esterase and a preparation method of the three-component metal aerogel nano-enzyme. The preparation method of the three-component metal aerogel nano-enzyme comprises the following steps: dissolving four metal salts in absolute ethyl alcohol according to a ratio, and ultrasonically mixing uniformly; preheating in a water bath, adding a freshly prepared sodium borohydride ethanol solution, quickly stirring, and standing to obtain three-component metal organic gel; and after multiple times of solvent exchange is conducted through acetone, supercritical carbon dioxide drying is conducted, and the three-component metal aerogel nano-enzyme is obtained. The preparation method of the three-component metal aerogel nano-enzyme has the characteristics of relatively simple process, mild reaction conditions and no surfactant; the three-component metal aerogel nano-enzyme prepared by the method has a self-supporting three-dimensional porous structure, has excellent peroxidase-like activity, and can be used for detecting the activity of acetylcholin esterase.

The invention provides a method for rapidly detecting pesticide residues in fruits and vegetables, and belongs to the field of pesticide analysis and detection. The method comprises the following steps: mixing a copper nanoparticle / reduced graphene oxide / chitosan composite material, acetylcholin esterase, choline oxidase and a cross-linking agent glutaraldehyde solution for reaction to obtain a double-enzyme functionalized nano composite material; dispensing a chitosan solution on the surface of a working electrode of a screen-printed electrode, drying to form a film, and covalently modifying the surface of the electrode with the bienzyme functionalized nano composite material through a cross-linking agent glutaraldehyde to obtain a sensing electrode; according to the method, acetylcholine is taken as a substrate, a sensing electrode is used, a chronoamperometry is adopted, inhibition ratios of pesticide standard substance solutions with different known concentrations to electrode response current are detected, and a standard curve between the pesticide concentration and the current inhibition ratio is established; and calculating the pesticide residue concentration in the fruit and vegetable sample to be detected according to the current inhibition ratio of the liquid to be detected and the standard curve. Therefore, the sensitivity and accuracy of pesticide residue detection in fruits and vegetables are improved.

The application provides a kind of gold nanoclusters with polypeptide as ligand and detection method of methyl parathion, the sequence of polypeptide in the gold nanoclusters with polypeptide as ligand is H (S, D) GGFFYCC;The gold nanoclusters with polypeptide as ligand can be used for double signal detection of methyl parathion and detection of methyl parathion in fruits and vegetables.Fluorescence signal detection: gold nanoclusters are added to the test solution, 37 DEG C reaction 30-60 min, detect the fluorescence change at 700 nm.Colorimetric signal: in pH=8.0 buffer solution, different concentrations of methyl parathion, acetylcholinesterase, choline oxidase and acetylcholine are added, 37 DEG C preheating 30-60 min.pH=4.0 buffer solution, gold nanoclusters and TMB are added, 37 DEG C reaction 10 min, 652 nm is measured absorbance.The detection method of the application has high detection sensitivity, strong anti-interference ability, low detection limit, high correlation coefficient of detection curve, good reproducibility in fruit and vegetable detection recovery experiment, and has good practical value in the detection of methyl parathion.

The invention belongs to the technical field of natural product extraction and fermentation engineering, and particularly relates to a tobacco fermentation liquor extract with acetylcholin esterase inhibition and antioxidant activity as well as a preparation method and application of the tobacco fermentation liquor extract. Specifically, research finds that by taking tobacco leaves or tobacco waste as a substrate and screening a nicotine-resistant microbial strain with high fermentability for co-culture fermentation, acetylcholin esterase inhibition and antioxidant activity of metabolite from a single plant or microorganism source can be effectively improved; therefore, the tobacco fermentation liquor extract with acetylcholin esterase inhibition and antioxidant activity is obtained. Tobacco leaves or tobacco wastes are used as raw materials to prepare products with biomedical potential, and a new way is provided for promoting multipurpose utilization of tobaccos.

The invention discloses a composition for assisting in improving memory and application of the composition, and particularly relates to the technical field of health care products. The composition comprises broccoli leaf polypeptide, pleurotus nebrodensis polypeptide, gastrodia elata protein peptide and N-acetylneuraminic acid in a mass ratio of 1: (0.5-1.5): (2-3): (0.1-0.3). According to the invention, the four components cooperate to promote secretion of acetylcholine and reduce the content of acetylcholin esterase, so that the composition has the efficacy of assisting in improving memory and cognitive ability, and can be applied to health care products.

The application discloses a kind of compositions for assisting to improve memory and application thereof, and specifically relates to health product technical field.The composition includes broccoli leaf polypeptide, white mushroom polypeptide, gastrodin protein peptide and N-acetylneuraminic acid, and the mass ratio is 1:(0.5-1.5):(2-3):(0.1-0.3).The application promotes acetylcholine secretion by four components synergistically, reduces acetylcholinesterase content, so that the composition has the efficacy of assisting to improve memory and cognitive ability, and can be applied to health product.

The application relates to the technical field of material science, analytical chemistry and food safety detection technology, in particular to a ratio fluorescent carbon dot microneedle patch for detecting pesticide residues of fruits and vegetables, and a preparation method thereof. The preparation method comprises the following steps: Fe-CDs and MnO2 are prepared into Fe-CDs / MnO2 nanosheet hybrid materials; the Fe-CDs / MnO2 nanosheet hybrid materials, acetylcholine, acetylcholine esterase and o-phenylenediamine are added into a PVA solution under vacuum conditions; a solvent and a crosslinking agent are further added; after the reaction is completed, the Fe-CDs / MnO2 / PVA microneedle patch is obtained by pouring into a microneedle patch mold and drying. The microneedle patch can realize rapid, accurate, on-site operable and low-cost detection of pesticide residues of fruits and vegetables in combination with RGB color recognition software.

The invention belongs to the technical field of natural medicines and pharmacology, and particularly relates to a raspberry extract as well as a preparation method and application thereof. Aiming at the problems of brain fatigue and functional hypomnesia caused by high-intensity brainwork of middle-aged and young people below 50 years old in the modern society, the invention provides a preparation process of a targeted enriched active monomer, which comprises the following steps: by taking dried raspberry fruits as a raw material, firstly, carrying out reflux degreasing with n-hexane to remove fat-soluble impurities; and sequentially extracting the degreased filter residues step by step by using ethanol with different concentrations, combining extracting solutions, and concentrating and drying to obtain the traditional Chinese medicine composition. The extract obtained by the preparation process can remarkably improve dysmnesia induced by scopolamine and inhibit the activity of acetylcholin esterase, is suitable for preparing products for relieving mental fatigue and improving learning efficiency, and does not relate to treatment of degenerative diseases such as senile dementia.

The invention belongs to the technical field of microorganisms, and particularly relates to a new strain of microbacterium and application thereof. The bacterial strain is separated from rapana venosa, is a new species of Microbacterium sp. M-28, is registered and preserved in the China Center for Type Culture Collection (CCTCC), and has a preservation number of CCTCC NO: M 20241861. The invention also discloses a method for preparing the bacterial strain. The microbacterium sp. M-28 strain disclosed by the invention has obvious bacteriostatic activity on bacillus subtilis, shewanella, staphylococcus aureus and proteusbacillus vulgaris, has obvious acetylcholin esterase inhibitory activity, has obvious antioxidant activity, has alpha-glucosidase inhibitory activity, can generate casein enzyme and amylase, and can be used for preparing a food additive. The strain can be applied to preparation of a bacteriostatic agent, an acetylcholin esterase inhibitor, an antioxidant, an alpha-glucosidase inhibitor, casein and amylase, can also replace the bacteriostatic agent, the antioxidant, the casein and the amylase in a viable form to be applied to industrial production, and has a very good development and application prospect.

The invention relates to the technical field of agricultural green prevention and control, in particular to a gradient prevention and control system for corn diseases and insect pests based on Chenopodium amansii-Artemisia desertorum synergetic allelopathy. Through a pi-pi conjugate synergetic mechanism (the synergetic index is larger than or equal to 1.83) of Chenopodium amansii alkaloid and Artemisia desertorum flavone, the inhibition activity IC50 of corn borer acetylcholin esterase is reduced to 9.3 mu M from 28 mu M of a single allelopathic substance, and the inhibition activity IC50 of corn borer acetylcholin esterase is reduced to 9.3 mu M; four layers of sustained-release granules (the lasting period is longer than or equal to 60 days and is prolonged by 300% compared with that of a conventional biopesticide) and Beidou intelligent application (the CV value is smaller than or equal to 8.2%) are combined, the corn borer control effect is 95.3%, the stem boring rate is only 4.7%, and zero chemical pesticide input is achieved in the whole process; meanwhile, soil residues can be avoided through the full-degradable corncob carrier (degradation for 182 + / -5 days) and the polylactic acid outer layer, the diversity of soil fungi after application is improved by 28.4%, the survival rate of earthworms is 100%, the technical contradiction between high prevention effect and high pollution and long-term effect and eco-friendliness is solved, and the requirements of green agriculture are met.

The invention relates to a piperidine alkoxy pyridine compound as well as a preparation method and application thereof. The general formula of the chemical structure of the piperidine alkoxy pyridine compound is shown as a formula (I), wherein R1 is selected from one of C1-6 alkyl, benzyl, substituted benzyl and picolyl, the substituted benzyl is benzyl of which the benzene ring is substituted by 1-4 substituents, and the substituents are independently selected from one of halogen, C1-4 alkyl, C1-4 alkoxy, N (CH3) 2, NO2, NHCOCH3 and CN respectively; r2 is selected from H or halogen; and n is an integer selected from 1-4. The piperidine alkoxy pyridine compound or the pharmaceutically acceptable salt of the piperidine alkoxy pyridine compound has relatively strong inhibitory activity on acetylcholin esterase and A beta 1-42 oligomer, and also has anti-neuroinflammation activity at the same time.

The invention discloses a hypericum perforatum extract insecticide for preventing and treating plutella xylostella and application of the hypericum perforatum extract insecticide, and belongs to the technical field of agriculture. Firstly, a hypericum perforatum ethanol crude extract is prepared and extracted with ethyl acetate to obtain an ethyl acetate extraction fraction with the effect of preventing and treating plutella xylostella; furthermore, on the basis of the biological affinity effect of the acetylcholin esterase, the trypsin and small molecule ligands in the extraction fractions of the ethyl acetate, insecticidal active compounds are screened, and the screened multiple active components have potential acetylcholin esterase and trypsin multi-target inhibitory activity. Animal experiment results show that the botanical insecticide prepared by taking luteolin-7-O-glucoside, quercetin or ethyl acetate extraction fractions as main functional components has an excellent plutella xylostella prevention and treatment effect, is environment-friendly and does not generate secondary pollution. The invention provides an important strategy for the development of biopesticides taking acetylcholin esterase and trypsin as multiple targets.

The invention relates to the field of medicinal chemistry, and particularly discloses a piperidine derivative, a pharmaceutical composition, application of the piperidine derivative and a preparation method of the piperidine derivative. The piperidine derivative has a structure shown in a general formula (I). The piperidine derivative can significantly improve the learning and memory ability of A beta-induced Alzheimer's disease model rats and effectively reduce the activity of acetylcholin esterase in brain tissues, and provides a potential candidate for developing novel Alzheimer's disease treatment drugs.

The invention relates to a paper-based sensor, a preparation method of the paper-based sensor and application of the paper-based sensor in detection of alternariol, and belongs to the technical field of food detection. The paper-based sensor comprises a first protective layer, an immobilization layer, a substrate layer, a color development layer and a second protective layer, the surfaces of the first protective layer and the second protective layer are coated with hydrophobic materials; central areas of the immobilization layer, the substrate layer and the color development layer are coated with acetylcholin esterase, acetylthiocholine and nano-enzyme respectively to form hydrophilic reaction areas, and non-hydrophilic reaction areas are coated with hydrophobic materials to form hydrophobic isolation areas; the first protection layer, the immobilization layer, the substrate layer, the color development layer and the second protection layer are folded according to a preset sequence, and the hydrophilic reaction areas of the immobilization layer, the substrate layer and the color development layer are mutually overlapped after folding. The paper-based sensor is remarkably improved in the aspects of detection sensitivity, stability and convenience.

The invention discloses a construction method and application of a smartphone-assisted organophosphorus pesticide detection sensor, and belongs to the technical field of analysis and detection. The method comprises the following steps: firstly, synthesizing UIO-66 (at) MnO2 nano-enzyme which has relatively high oxide-like enzyme activity so as to catalytically oxidize 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) to develop color; acetylcholin esterase is utilized to catalyze hydrolysis of acetylthiocholine to generate reducing substances, the chromogenic reaction of TMB is inhibited, and the organophosphorus pesticide can specifically inhibit the activity of acetylcholin esterase, so that the chromogenic reaction is recovered; the reaction solution is shot through Color Grab software of the smart phone, an RGB value is output, a gray value is calculated, a linear relation between the gray value and the pesticide concentration is established, and quantitative detection of the organophosphorus pesticide is achieved. The sensor constructed by the invention is high in sensitivity, good in selectivity, simple and convenient to operate and low in cost, can be successfully applied to rapid detection of organophosphorus pesticides in food and environmental samples, and has a good practical application prospect.

The invention discloses a substituted coumarin-eugenol derivative as well as a preparation method and application thereof. The derivative is prepared by connecting a coumarin mother nucleus and eugenol through a flexible chain by virtue of a two-step nucleophilic substitution reaction. The derivative disclosed by the invention is novel in structure, can simultaneously and effectively inhibit monoamine oxidase-B and acetylcholin esterase and inhibit A beta protein aggregation, has excellent oxidation resistance and metal ion chelation capability, and realizes a multi-target synergistic effect. In-vitro and in-vivo experiments show that the compound has a remarkable improvement effect on a plurality of pathological links such as Alzheimer's disease, and is low in neurotoxicity and high in safety. Meanwhile, the preparation method has the advantages of easily available raw materials and simple steps, is suitable for industrial production, and has a wide application prospect in the aspect of preparing the anti-neurodegenerative disease medicine.

The present invention relates to a hybrid pyrano[2,3-c]pyrazole-pyrazolones and analogous compounds of Formula 3; and a process for the preparation thereof. The invention further relates to identification of bioactivities of synthesized novel hybrid pyrano[2,3-c]pyrazole- pyrazolones. The novel hybrid pyrano[2,3-c]pyrazole-pyrazolones of Formula 3 displayed useful neuroprotective property and inhibited acetylcholine esterase enzyme at the lowest concentration. These compounds were also found to cross the Blood-Brain Barrier.

The invention discloses a method for detecting acetylcholin esterase by using a multi-hot-spot active targeting type SERS (Surface Enhanced Raman Scattering) substrate, which comprises the following steps: soaking the multi-hot-spot active targeting type SERS substrate in a solution to be detected, taking out after sufficient time, and carrying out SERS detection; the preparation method of the multi-hotspot active targeting type SERS substrate comprises the following steps: preparing a gold nanosphere superlattice two-dimensional membrane, synthesizing gold and silver double-component hollow nanoparticles, attaching the gold nanosphere superlattice two-dimensional membrane to a silicon wafer, modifying a beta-cyclodextrin-methylene blue inclusion compound, and adding the gold and silver double-component hollow nanoparticles. According to the method, the beta-cyclodextrin-methylene blue inclusion compound-gold nanosphere superlattice two-dimensional film-silicon wafer can be prepared firstly, after the beta-cyclodextrin-methylene blue inclusion compound-gold nanosphere superlattice two-dimensional film-silicon wafer is combined with an object to be detected, the gold-silver double-component hollow nanoparticles are added, and SERS detection is conducted. When the method is used for detecting the acetylcholin esterase, the sensitivity, the accuracy and the repeatability of a detection result can be improved.

The invention discloses a preparation method and application of a novel acetylcholin esterase activated curcumin-based fluorescent probe for detecting diazinon. The fluorescent probe is 4, 6-bis [(1E)-2-(4-(((dimethylamino) carbonyl) oxy)-2-methoxyphenyl) vinyl]-2, 2-difluoro-2H-1, 3, 2-dioxo boron heterocyclic hexene tetrafluoroborate, which is called YPC for short. The probe YPC can specifically recognize acetylcholin esterase, after acetylcholin esterase is added, orange fluorescence of a solution is remarkably enhanced, meanwhile, due to the inhibition effect of diazinon on the activity of acetylcholin esterase, the orange fluorescence of the probe is quenched, and therefore detection of diazinon is achieved. The detection limit of the probe YPC to acetylcholin esterase is as low as 5.2114 mU / mL, the detection limit of the probe YPC to diazinon is as low as 1.0463 ng / mL, and the probe YPC shows a good linear relationship in the range of 0-12 ng / mL.

The invention discloses an application of a scopolamine model in an acetylcholin esterase inhibitor, and relates to the technical field of medicines.The application comprises the steps that S1, the learning memory of the scopolamine model is improved through a compound; s2, the inhibition effect of the compound on acetylcholin esterase is verified, and the compound is specifically (5S)-1-(3, 4-dihydroxyphenyl)-5-hydroxy-7-(4-hydroxy-3-methoxyphenyl) heptyl-3-ketone. A plurality of groups of experiments are designed to prove that the compound can remarkably improve the learning and memory dysfunction of AD model mice induced by scopolamine and has dose dependence, an enzyme activity inhibition experiment shows that the compound has strong inhibitory activity on AChE, the half inhibitory concentration (IC50) of the compound reaches 5.5 nM and is more remarkable than that of a clinical first-line anti-AD drug donepezil (IC50 = 12.2 nM), and the compound can be applied to preparation of anti-AD drugs. As the AChE activity increase is a key factor of cholinergic system injury in the AD pathological process, the compound is speculated to reduce hydrolysis of acetylcholine by inhibiting the AChE activity, and further improve the learning and memory ability of model animals.

The present invention relates to pesticide mixtures comprising as active compound I a carboxamide derivative active as an insecticide and at least one active compound II selected from group M comprising acetylcholine esterase inhibitors, GABA-regulated chloride channel antagonists, sodium channel modulators, nicotinic acetylcholine receptor agonists / antagonists, allosteric nicotinic acetylcholine receptor activators, chloride channel activators, juvenile hormone mimics, homopteran feeding blockers, mite growth inhibitors, mitochondrial ATP synthase inhibitors, oxidative phosphorylation uncouplers, chitin biosynthesis inhibitors, molting disruptors, ecdyson receptor agonists, octamine receptor agonists, MET inhibitors, voltage-gated sodium channel blockers, lipid synthesis inhibitors,ryanodine receptor modulators and other compounds defined in the description, in synergistically effective amounts. The invention also relates to methods and the use of these mixtures to combat and control insects, arachnids, or nematodes in plants, and to protect these plants against pest infestation, in particular also to protect plant propagation material, such as seeds.