Application of fermented Lactobacillus mucilaginosus IOB802 in improving memory

Abstract

The present invention provides an application of fermented Lactobacillus mucilaginosus IOB802 in improving memory, and relates to the field of microorganisms. The bacterial powder of fermented Lactobacillus mucilaginosus IOB802 and its postbiotics significantly increase the acetylcholine level of cognitive impairment mice and reduce the acetylcholinesterase level; and the serum superoxide dismutase is significantly increased, and the malondialdehyde level is significantly reduced. Therefore, the bacterial powder of fermented Lactobacillus mucilaginosus IOB802 and its postbiotics can alleviate the oxidative damage level of mice, thereby alleviating nerve damage, and have the effect of improving memory.

Description

Technical Field

[0001] The invention relates to the field of microorganisms, and in particular to application of fermented mucus lactobacillus IOB802 in improving memory. Background Art

[0002] As the pace of life in modern society accelerates, people are facing increasing psychological pressure and cognitive load, leading to more and more common problems such as memory loss, anxiety and depression. Therefore, finding safe and effective non-drug methods to improve these conditions has become a research hotspot.

[0003] In recent years, scientific research has revealed a close connection between intestinal microbes and brain function, a connection known as the "brain-gut axis." Intestinal microbes can affect cognitive function, mood regulation, and memory by influencing the production of neurotransmitters, regulating the immune system, and communicating directly through the vagus nerve. Acetylcholine (ACh) is one of the most important neurotransmitters in the central nervous system and has a significant effect on the learning and memory process. It works by binding to specific receptors and being broken down by acetylcholinesterase (AChE). Studies have shown that the level of acetylcholine in the brain is closely related to memory ability. In the brain, acetylcholine is mainly produced in the hippocampus and basal forebrain, and plays a role in a variety of cognitive functions, including memory, attention, arousal, and motivation.

[0004] In addition, the acetylcholinergic system regulates memory and hippocampal plasticity through interactions with non-neuronal cells, such as microglia and astrocytes, which express α7 nAChRs and are able to reduce the release of inflammatory cytokines, thereby preventing memory impairment caused by inflammation.

[0005] At the same time, fermented lactobacillus has been found to regulate the composition of intestinal microorganisms, promote the growth of beneficial bacteria, and inhibit harmful bacteria, thereby maintaining intestinal health. Postbiotics, as bioactive substances produced by inactivated probiotics, can affect the nervous system through the gut-brain axis. A healthy intestinal environment helps absorb nutrients and reduce inflammatory responses, which is crucial for brain health and cognitive ability.

[0006] In summary, acetylcholine and its receptors play an important role in the nervous system and memory. The development of a probiotic and its postbiotic powder that can not only improve memory, but also regulate intestinal flora and enhance physical fitness is of great significance to society. Summary of the invention

[0007] In view of the technical problems existing in the prior art, the present invention aims to provide an application of fermented mucus lactobacillus IOB802 in improving memory. The fermented mucus lactobacillus IOB802 is classified and named as fermented mucus lactobacillus Limosilactobacillus fermentum , the deposit number is CGMCC NO.23120.

[0008] Preferably, the fermented Lactobacillus mucilaginosus IOB802 has the effects of increasing the level of acetylcholine and reducing the level of acetylcholinesterase.

[0009] Preferably, the fermented Lactobacillus mucilaginosus IOB802 can be used to prepare products for improving memory.

[0010] Preferably, the fermented Lactobacillus mucilaginosus IOB802 can be used to prepare fermented Lactobacillus mucilaginosus IOB802 bacterial powder and fermented Lactobacillus mucilaginosus IOB802 postbiotic powder.

[0011] Preferably, the raw materials of the product for improving memory include fermented Lactobacillus mucilaginosus IOB802 powder or fermented Lactobacillus mucilaginosus IOB802 postbiotic powder.

[0012] Preferably, the method for preparing the fermented Lactobacillus mucilaginosus IOB802 powder and postbiotics comprises the following steps:

[0013] (1) Strain activation: Inoculate the strain into a liquid culture medium at a rate of 1%, and culture it at 30-37°C in a closed environment for 24-36 hours to obtain an activated seed solution. Inoculate the activated seed solution into an activation culture medium at a rate of 3%, and culture it at 30-37°C in a closed environment for 24-36 hours to obtain a fermentation seed solution.

[0014] (2) Fermentation: Inoculate the cultured secondary seed liquid into the liquid culture medium at an inoculation rate of 3%. The fermentation tank is stirred at a speed of 50-70 r / min, at 30-37°C, and cultured in a closed manner for 10-12 h. During the fermentation process, food-grade sodium hydroxide is added to supplement the alkali to ensure that the pH of the fermentation liquid is maintained at 6.0-6.4.

[0015] (3) Preparation of bacterial powder: After centrifugation of the fermentation medium, the bacterial sludge was obtained and vacuum freeze-dried to obtain the fermented Lactobacillus mucilaginosus IOB802 bacterial powder. The freeze-drying conditions were pre-freezing for 5 to 8 hours, vacuum freeze drying for 36 to 40 hours, and a vacuum degree of 8.0 to 10.0 Pa;

[0016] (4) Preparation of postbiotic powder: The fermentation liquid is inactivated at 85°C for 15 min. After inactivation, the materials are mixed evenly and spray-dried. The spray-drying conditions are an inlet air temperature of 170-200°C and an outlet air temperature of 60-80°C.

[0017] Preferably, the liquid culture medium comprises: 10.0 g / L peptone, 9.0 g / L yeast powder, 10 g / L glucose, 10 g / L stachyose, 1.0 mL / L Tween 80, 2.0 g / L dipotassium hydrogen phosphate, 2.0 g / L ammonium citrate, 0.2 g / L magnesium sulfate, 0.05 g / L manganese sulfate and 0.05 g / L calcium chloride.

[0018] Preferably, the viable count of the fermented Lactobacillus mucilaginosus IOB802 powder is 2×10 10 ~4×10 10 cfu / g.

[0019] Beneficial effects of the present invention:

[0020] The present invention provides an application of fermented mucus lactobacillus IOB802 in improving memory. The bacterial powder and postbiotics prepared by the bacteria significantly increase the acetylcholine (Ach) level of cognitive impairment mice and reduce the acetylcholinesterase (AchE) level. The increase of acetylcholine can enhance the communication between neurons, making the transmission of information in the brain smoother. When the activity of acetylcholinesterase is reduced, the retention time of acetylcholine in the synaptic cleft is prolonged, which makes the transmission of nerve signals more durable and helps to strengthen the memory and learning process, which is crucial for the formation of short-term memory and the consolidation of long-term memory. In addition, the serum superoxide dismutase (SOD) level is significantly increased and the malondialdehyde (MDA) level is significantly reduced. When the serum superoxide dismutase activity is increased, free radicals can be cleared faster and more effectively, reducing their oxidative damage to neurons. When the malondialdehyde level is reduced, it indicates that the oxidative damage is reduced and the structure and function of the cell membrane are protected.

[0021] Oxidative stress can lead to decreased neuronal function and even cell apoptosis, and elevated serum superoxide dismutase helps protect neurons. Serum superoxide dismutase can also promote synaptic plasticity, helping the brain better adapt and integrate new information, which is essential for learning and memory.

[0022] Therefore, the bacterial powder of fermented Lactobacillus mucilaginosus IOB802 and its postbiotics can alleviate the level of oxidative damage in mice, thereby alleviating nerve damage and having the effect of improving memory. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a trend graph of escape latency in mice;

[0024] Figure 2 is the total distance of the second quadrant;

[0025] Figure 3 is the total time in the target quadrant;

[0026] Figure 4is the number of times the platform is crossed;

[0027] Figure 5 The movement trajectory of the mouse;

[0028] Figure 6 is the acetylcholine level in mice;

[0029] Figure 7 is the acetylcholinesterase level in mice;

[0030] Figure 8 is the malondialdehyde level;

[0031] Fig. 9 is the serum superoxide dismutase level;

[0032] Fig.10 This is a slice of the hippocampus of mouse brain tissue.

[0033] The fermented mucus lactobacillus IOB802 used in the present invention is classified and named as fermented mucus lactobacillus Limosilactobacillus fermentum , was deposited on August 5, 2021 in the General Microbiology Center of China Microorganism Culture Collection, referred to as CGMCC, with the address at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO.23120. DETAILED DESCRIPTION

[0034] The present invention provides an application of fermented mucus lactobacillus IOB802 in improving memory. The fermented mucus lactobacillus IOB802 is classified and named as fermented mucus lactobacillus Limosilactobacillus fermentum , was deposited on August 5, 2021 in the General Microbiology Center of China Microorganism Culture Collection, referred to as CGMCC, with the address at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO.23120.

[0035] In a preferred embodiment of the present invention, the fermented Lactobacillus mucilaginosus IOB802 has the effects of increasing the level of acetylcholine and reducing the level of acetylcholinesterase.

[0036] In a preferred embodiment of the present invention, the fermented Lactobacillus mucilaginosus IOB802 can be used to prepare products for improving memory.

[0037] In a preferred embodiment of the present invention, the fermented Lactobacillus mucilaginosus IOB802 can be used to prepare fermented Lactobacillus mucilaginosus IOB802 bacterial powder and fermented Lactobacillus mucilaginosus IOB802 postbiotic powder.

[0038] In a preferred embodiment of the present invention, the raw materials of the product for improving memory include fermented Lactobacillus mucilaginosus IOB802 powder or fermented Lactobacillus mucilaginosus IOB802 postbiotic powder.

[0039] In a preferred embodiment of the present invention, the method for preparing the fermented Lactobacillus mucilaginosus IOB802 powder and postbiotics comprises the following steps:

[0040] (1) Strain activation: Inoculate the strain into a liquid culture medium at a rate of 1%, and culture it at 30-37°C in a closed environment for 24-36 hours to obtain an activated seed solution. Inoculate the activated seed solution into an activation culture medium at a rate of 3%, and culture it at 30-37°C in a closed environment for 24-36 hours to obtain a fermentation seed solution.

[0041] (2) Fermentation: Inoculate the cultured secondary seed liquid into the liquid culture medium at an inoculation rate of 3%. The fermentation tank is stirred at a speed of 50-70 r / min, at 30-37°C, and cultured in a closed manner for 10-12 h. During the fermentation process, food-grade sodium hydroxide is added to supplement the alkali to ensure that the pH of the fermentation liquid is maintained at 6.0-6.4.

[0042] (3) Preparation of bacterial powder: After centrifugation of the fermentation medium, the bacterial sludge was obtained and vacuum freeze-dried to obtain the fermented Lactobacillus mucilaginosus IOB802 bacterial powder. The freeze-drying conditions were pre-freezing for 5 to 8 hours, vacuum freeze drying for 36 to 40 hours, and a vacuum degree of 8.0 to 10.0 Pa;

[0043] (4) Preparation of postbiotic powder: The fermentation liquid is inactivated at 85°C for 15 min. After inactivation, the materials are mixed evenly and spray-dried. The spray-drying conditions are an inlet air temperature of 170-200°C and an outlet air temperature of 60-80°C.

[0044] In a preferred embodiment of the present invention, the liquid culture medium comprises: 10.0 g / L peptone; 9.0 g / L yeast powder; 10 g / L glucose; 10 g / L stachyose; 1.0 mL / L Tween 80; 2.0 g / L dipotassium hydrogen phosphate; 2.0 g / L ammonium citrate; 0.2 g / L magnesium sulfate; 0.05 g / L manganese sulfate; and 0.05 g / L calcium chloride.

[0045] In a preferred embodiment of the present invention, the liquid culture medium comprises: 10.0 g / L peptone; 9.0 g / L yeast powder; 10 g / L glucose; 10 g / L stachyose; 1.0 mL / L Tween 80; 2.0 g / L dipotassium hydrogen phosphate; 2.0 g / L ammonium citrate; 0.2 g / L magnesium sulfate; 0.05 g / L manganese sulfate; and 0.05 g / L calcium chloride.

[0046] In a preferred embodiment of the present invention, the viable count of the fermented Lactobacillus mucilaginosus IOB802 powder is 2×10 10 ~4×10 10 cfu / g.

[0047] Example 1

[0048] Preparation of fermented Lactobacillus mucilaginosus IOB802 powder and postbiotic powder

[0049] The preparation method is as follows:

[0050] (1) Strain activation: Inoculate the strain into a liquid culture medium at a rate of 1%, and culture it at 37±2℃ in a closed state. Observe the colony morphology and bacterial characteristics at different culture times. The culture requires that the seeds be uniform, consistent in morphology, and strong. The seeds that have been cultured for 24 hours are more in line with the conditions, so the optimal culture time for seeds is determined to be 24 hours. The activated seed liquid is then inoculated into the activation culture medium at a rate of 3%, and the culture conditions are 37±2℃. Culture it in a closed state for 24 hours to obtain the fermented seed liquid.

[0051] (2) Fermentation: The cultured secondary seed liquid was inoculated into the liquid culture medium at an inoculation rate of 3%. The fermentation tank was stirred at a speed of 50-70 r / min and cultured in a closed environment at 37°C for 12 h. During the fermentation process, food-grade sodium hydroxide was added to supplement the alkali to ensure that the pH of the fermentation liquid was maintained at 6.2±0.2.

[0052] (3) Preparation of bacterial powder: After centrifugation of the fermentation medium, the bacterial sludge was obtained and vacuum freeze-dried to obtain the fermented mucus Lactobacillus IOB802 bacterial powder. The freeze-drying conditions were pre-freezing at -40°C for 6 hours, freeze-drying for 36±2 hours, and freeze-drying was completed when the vacuum degree dropped to 8.0Pa;

[0053] (4) Preparation of postbiotic powder: The fermentation broth was inactivated at 85°C for 15 min. After inactivation, the materials were mixed evenly and then spray-dried. The spray-drying conditions were an inlet air temperature of 175°C and an outlet air temperature of 70°C.

[0054] The liquid culture medium components are: peptone 10.0 g / L; yeast powder 9.0 g / L; glucose 10 g / L; stachyose 10 g / L; Tween 80 1.0 mL / L; dipotassium hydrogen phosphate 2.0 g / L; ammonium citrate 2.0 g / L; magnesium sulfate 0.2 g / L; manganese sulfate 0.05 g / L; calcium chloride 0.05 g / L.

[0055] Example 2

[0056] The memory-improving effects of fermented Lactobacillus mucilaginosus IOB802 powder and fermented Lactobacillus mucilaginosus IOB802 postbiotic powder on cognitively impaired mice.

[0057] (a) Experimental materials:

[0058] Fermented Lactobacillus mucilaginosus IOB802 fermentation postbiotic powder, fermented Lactobacillus mucilaginosus IOB802 powder.

[0059] Experimental mice: 4-week-old SPF male KM mice, weighing 20±2g.

[0060] (b) Mouse modeling and grouping

[0061] Fifty SPF male KM mice aged 4 weeks and weighing 20±2 g were randomly divided into 5 groups (10 mice in each group): blank group, model group, IOB802 group, IOB802 epigenetic group, and positive group, with 10 mice in each group. This experiment was approved by the Animal Ethics Committee with the license number SY×K (Tianjin) 2021-0003.

[0062] The mice in the blank group were gavaged with 200 μL PBS solution at the same time every day for 30 days. The mice in the model group were gavaged with 200 μL PBS solution at the same time every day for 30 days. The mice in the IOB802 group were gavaged with 200 μL 3×10 9 CFU / ml of fermented Lactobacillus mucilaginosus IOB802 powder was continued for 30 days. Mice in the IOB802 postbiotic group were gavaged with 200 μL of 3×10 9 The mice in the positive drug group were intragastrically administered with 200 μL of piracetam at a dose of 100 mg / kg at the same time period every day.

[0063] The experiment was started after 7 days of adaptation. The mice in each group were gavaged at the same time every day according to the gavage method of each group for 30 days. After gavage, the model group, IOB802 group, IOB802 postbiotic group, and positive group were intraperitoneally injected with scopolamine (2 mg / kg according to the body weight of the mouse) on the second day to establish the mouse cognitive impairment model. The blank control group was injected with an equal volume of normal saline, and the modeling and behavioral experiments were performed 20 minutes later.

[0064] (c) Mouse water maze experiment

[0065] On the second day after the last oral gavage, each group was injected with scopolamine or saline for 20 minutes before a water maze test. The experiment was divided into two parts: ① Positioning navigation test: training was conducted for 5 consecutive days, with four different starting points (different quadrants) trained every day. The time to find the platform was recorded, and the time it took the rat to find the platform was recorded as the latency (if the platform was not found for more than 60 s, the latency was recorded as 60 s). ② Spatial exploration test: 24 hours after the end of the positioning navigation test, the underwater platform was removed, and the rat was placed in the water at the same entry point. The swimming path of the rat within 60 s, the number of times the rat crossed the platform, and the time it stayed in the target quadrant were recorded to judge the rat's memory ability. Figure 1 This is the trend chart of the escape latency of mice. Figure 2 is the total distance in the second quadrant, Figure 3 is the total time in the target quadrant, Figure 4 is the number of times the platform is crossed.

[0066] like Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, the escape latency of the IOB802 group and the IOB802 epigenetic group decreased, and the total distance in the second quadrant, the total time in the target quadrant, and the number of times the platform was crossed increased compared with the model group. The difference between the IOB802 epigenetic group and the blank control group was small, but there was a significant difference compared with the model group. This shows that the mice in the treatment group have a memory of the original platform position, increased the exploration time and distance in the target quadrant, and also increased the number of crossings in the target area, which has a significant improvement on the memory ability of the mice.

[0067] The movement trajectory of the mouse is as follows Figure 5 As shown in the figure, the trajectory images show that the mice in the model group spend most of their time circling the water maze, without purposefully searching for the hidden platform area, and their spatial cognitive ability is impaired. The blank group has significantly more exploration behaviors for the hidden platform location area and location than the model group, indicating that the modeling has caused cognitive impairment. The IOB802 group and the IOB802 epigenetic group have significantly more exploration behaviors for the hidden platform location area and location than the model group, and the effect of the IOB802 epigenetic group is more obvious. This shows that IOB802 and its epigenetic have a significant improvement effect on cognitive ability.

[0068] Example 3

[0069] Fermented Lactobacillus mucilaginosus IOB802 powder and fermented Lactobacillus mucilaginosus IOB802 postbiotic powder can improve the antioxidant capacity of neurons and reduce oxidative stress damage.

[0070] Table 1 Experimental materials

[0071]

[0072] Blood was collected from the eyeballs of mice. The mice were killed by dislocating the neck, and the brain, liver, colon contents and cecum contents were removed on ice and placed in a -80 degree refrigerator for quick freezing. The brain of the mouse was divided into two halves, one half was frozen in liquid nitrogen, and the other half was placed in paraformaldehyde tissue fixative for subsequent tissue sectioning and HE staining observation.

[0073] According to the instructions of the kit, the levels of acetylcholine (Ach) and acetylcholinesterase (AchE) in mouse brain tissue and superoxide dismutase (SOD) and malondialdehyde (MDA) in serum were measured. The test data are shown in Tables 2, 3, 4 and 5. The hippocampal slices of mouse brain tissue were also analyzed. Figure 6 is the acetylcholine level in mice, Figure 7 is the acetylcholinesterase level in mice, Figure 8 is the malondialdehyde level, Fig. 9 is the serum superoxide dismutase level, Fig.10 This is a slice of the hippocampus of mouse brain tissue.

[0074] like Figure 6 and Figure 7 As shown, compared with the blank group, the acetylcholine level in the model group was downregulated, indicating that the injection of scopolamine caused an imbalance in the acetylcholine function of mice; compared with the model group, the acetylcholine levels in the IOB802 group and the IOB802 postbiotic group were significantly increased, by 23.15% and 72.08%, respectively, and the acetylcholinesterase activity was decreased (P<0.05), by 60.01% and 29.66%, respectively. The increase in acetylcholine content can improve memory impairment, among which the effect of the IOB802 postbiotic group was more obvious.

[0075] Acetylcholine level increase rate = (experimental group - model group) / model group × 100%

[0076] The reduction rate of acetylcholinesterase level = (model group - experimental group) / model group × 100%, where the experimental group included the IOB802 group or the IOB802 postbiotic group.

[0077] Table 2 Acetylcholine levels in mouse brain tissue (μg / mgprot)

[0078]

[0079] Table 3 Acetylcholinesterase levels in mouse brain tissue (U / mg)

[0080]

[0081] like Figure 8 and Fig. 9 As shown in the figure, compared with the blank group, the serum superoxide dismutase activity in the model group was significantly reduced, indicating that the injection of scopolamine caused a strong impairment of antioxidant capacity. Oxidative damage in the brain may cause neuronal dysfunction and death, which is related to memory loss. Acetylcholine, as an important neurotransmitter in the central nervous system, is not only involved in the learning and memory process, but may also be related to oxidative damage.

[0082] The serum superoxide dismutase activity increased by 44.45% and 29.43% in the IOB802 group and IOB802 postbiotic group, respectively;

[0083] The malondialdehyde activity in the IOB802 group and IOB802 postbiotic group decreased by 44.19% and 32.53%, respectively.

[0084] Serum superoxide dismutase, as an important antioxidant enzyme, can effectively remove superoxide anions from cells, thereby reducing the damage of oxidative damage to cells. Malondialdehyde, as a product of oxidative stress, is usually used as a marker of oxidative damage. Increased malondialdehyde levels may cause oxidative damage to cell membranes, thereby affecting the function of neurons.

[0085] The formula for increasing superoxide dismutase activity in serum is: (experimental group - model group) / model group × 100%

[0086] The formula for reducing the MDA level in mouse serum was as follows: (model group - experimental group) / model group × 100%, where the experimental group included the IOB802 group or the IOB802 postbiotic group.

[0087] Table 4 Superoxide dismutase levels in mouse brain tissue serum (U / mL)

[0088]

[0089] Table 5 Malondialdehyde levels in mouse brain tissue (nmol / mL)

[0090]

[0091] The serum superoxide dismutase activity of the IOB802 group and the IOB802 postbiotic group was higher than that of the model group, indicating that taking probiotics and postbiotics would improve the antioxidant capacity of mice to a certain extent. The data results of the oxidative stress product malondialdehyde showed that the malondialdehyde content of the blank group was significantly lower than that of the model group, indicating that the model group had a higher level of peroxides and severe oxidative damage. The malondialdehyde levels of the IOB802 group and the IOB802 postbiotic group were significantly reduced, indicating that probiotics and postbiotics can alleviate the oxidative damage level of mice. This may be because acetylcholine can affect neuroimmune function through its anti-inflammatory effect, which may in turn affect memory problems caused by oxidative damage.

[0092] The hippocampus is a key area in the brain that is closely related to learning and memory functions, and the interaction between nerve cells and the acetylcholine system is crucial to the memory process. Fig.10 As shown in the figure, compared with the control group, the neurons in the hippocampus of mice were closely arranged and the cell morphology was relatively complete. The neurons in the hippocampus of the model group were scattered, the number of neurons was reduced, the nucleus was partially damaged, the color became lighter, and the cell morphology was also missing. After intervention with probiotics and postbiotics, the cell morphology and arrangement were well improved, and the damage to neurons was effectively inhibited. Acetylcholine can improve neuronal damage, regulate the activity pattern of the neural network in the hippocampus, and affect memory encoding and consolidation by activating specific receptors, such as α7 nicotinic acetylcholine receptors (α7 nAChRs). It further improved the neuronal damage caused by scopolamine.

[0093] In summary, the fermented Lactobacillus muciniphila IOB802 postbiotic powder of the present invention has a positive effect on cognitive ability, effectively improves the dysfunction of the cholinergic system caused by scopolamine, alleviates the level of oxidative damage in mice, and provides ideas for the development of new products of fermented Lactobacillus muciniphila IOB802 postbiotic powder.

[0094] No matter from which aspect the present invention is viewed, the above-mentioned embodiments of the present invention can only be regarded as an explanation of the present invention and cannot limit the present invention. The claims indicate the scope of the present invention, while the above-mentioned description does not indicate the scope of the present invention. Therefore, any changes within the meaning and scope equivalent to the claims of the present invention should be deemed to be included in the scope of the claims of the present invention.

Claims

1. An application of fermented Lactobacillus mucilaginosus IOB802 in the preparation of a product for improving memory by increasing acetylcholine levels and reducing acetylcholinesterase levels, characterized in that: The fermented mucus lactobacillus IOB802 is classified and named as fermented mucus lactobacillus Limosilactobacillus fermentum , the deposit number is CGMCC NO.23120.

2. The use according to claim 1, characterized in that The fermented Lactobacillus mucilaginosus IOB802 can be used to prepare fermented Lactobacillus mucilaginosus IOB802 bacterial powder and fermented Lactobacillus mucilaginosus IOB802 postbiotic powder.

3. The use according to claim 1, characterized in that The product raw materials include fermented mucus lactobacillus IOB802 bacterial powder or fermented mucus lactobacillus IOB802 postbiotic powder.

4. The use according to claim 3, characterized in that The preparation method of the fermented mucus lactobacillus IOB802 powder and postbiotic powder comprises the following steps: (1) Strain activation: Inoculate the strain into a liquid culture medium at a rate of 1%, and culture it at 30-37°C in a closed environment for 24-36 hours to obtain an activated seed solution. Inoculate the activated seed solution into an activation culture medium at a rate of 3%, and culture it at 30-37°C in a closed environment for 24-36 hours to obtain a fermentation seed solution. (2) Fermentation: Inoculate the cultured secondary seed liquid into the liquid culture medium at an inoculation rate of 3%. The fermentation tank is stirred at a speed of 50-70 r / min, at 30-37°C, and cultured in a closed manner for 10-12 h. During the fermentation process, food-grade sodium hydroxide is added to supplement the alkali to ensure that the pH of the fermentation liquid is maintained at 6.0-6.

4. (3) Preparation of bacterial powder: After centrifugation of the fermentation medium, bacterial sludge was obtained, and the fermented mucus Lactobacillus IOB802 bacterial powder was obtained after vacuum freeze drying; the freeze drying conditions were pre-freezing for 5 to 8 hours, vacuum freeze drying for 36 to 40 hours, and the vacuum degree was 8.0 to 10.0 Pa; (4) Preparation of postbiotic powder: The fermentation liquid is inactivated at 85°C for 15 min. After inactivation, the materials are mixed evenly and spray-dried to obtain fermented Lactobacillus mucilaginosus IOB802 postbiotic powder; the spray-drying conditions are an inlet air temperature of 170-200°C and an outlet air temperature of 60-80°C.

5. The use according to claim 4, characterized in that The liquid culture medium comprises: 10.0 g / L peptone, 9.0 g / L yeast powder, 10 g / L glucose, 10 g / L stachyose, 1.0 mL / L Tween 80, 2.0 g / L dipotassium hydrogen phosphate, 2.0 g / L ammonium citrate, 0.2 g / L magnesium sulfate, 0.05 g / L manganese sulfate and 0.05 g / L calcium chloride.

6. The use according to claim 3, characterized in that The viable count of the fermented Lactobacillus mucilaginosus IOB802 powder is 2×10 10 ~4×10 10 cfu / g.

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