382 results about "Malondialdehyde" patented technology

The invention relates to a technology system applicable to identification and evaluation of cold resistance of isolated parts of different bamboo varieties, in particular to a method for identifying and evaluating the cold resistance of bamboos. The method for identifying and evaluating the cold resistance of the bamboos is characterized by comprising the following steps of: a) taking a twig of the same bamboo age and with a length of 30 centimeters as a reference; b) quick-freezing a part of processed leaves in liquid nitrogen for 30 minutes and then storing the leaves into a refrigerator at the temperature of 80 DEG C below zero and the like. The method for identifying and evaluating the cold resistance of the bamboos is characterized by: a) conductivity determination; b) determination of soluble sugar content; c) determination of soluble protein content; d) malondialdehyde (MDA) determination; e) praline determination; f) determination of activities of superoxide dismutase (SOD); and g) determination of activities of peroxidase (POD).

The invention relates to a traditional Chinese medicine preparation for promoting lactation of pregnant sows, which is composed of astragalus, angelica, Codonopsis pilosula, Rhizoma Chuanxiong, Wang Buliuxing, Passepartout, motherwort and Cyperus cyperi. Significantly increase the serum prolactin (PRL) and growth hormone (GH) levels of lactating sows, promote the lactation performance of sows; increase the protein content in colostrum, promote the growth and development of sucking piglets; increase the serum complement C3 and immunoglobulin of sucking piglets (IgG, IgM, IgA) content and superoxidase (SOD) activity, reduce malondialdehyde (MDA) content, improve immune function and antioxidant function of suckling piglets.

The invention discloses sargassum pallidum polysaccharide selenium as well as a preparation method and an application thereof. The preparation method mainly comprises steps as follows: raw material pretreatment, hot water extraction, decoloration, deproteinization, freeze drying and selenylation reaction, wherein the selenylation reaction is implemented as follows: sargassum pallidum polysaccharide and dilute nitric acid are mixed and stirred at the room temperature, sodium selenite and barium chloride are added respectively, the mixture is heated to 60-80 DEG C, stirred and mixed uniformly toreact at the microwave power of 100-500 W for 3-5 min, a sargassum pallidum polysaccharide selenium solution is obtained, cooled to the room temperature, placed in a dialysis bag for dialysis and freeze-dried, and sargassum pallidum polysaccharide selenium is obtained. The preparation method is simple, the reaction condition is mild, the sargassum pallidum polysaccharide selenium is pollution-free and high in selenylation degree, has remarkable alpha-glucosidase inhibition activity, has the activity superior to that of acarbose and can remarkably improve the antioxidase activity of cells andreduce the content of malondialdehyde, a dietary supplement with antioxidant and blood sugar reducing efficacy can be developed, and the sargassum pallidum polysaccharide selenium can be applied to the field of food or medicines.

The orally taken antioxidant and antilipemic medicines include solid orally taken preparation prepared with forsythin and solid excipient in the weight ratio of 0.01-4 through mixing and conventionaltechnological process, and liquid orally taken preparation prepared with forsythin and liquid excipient in the weight ratio of 0.003-0.01 through mixing and conventional technological process. Test shows that forsythin as the active component of the present invention has excellent effect of clearing away hydroxy radical and can inhibit the oxidizing damage in tissue and cell organ level. The medicine of the present invention has also functions of delaying senility and reducing blood fat, etc.

The invention relates to a composite antioxidant for improving reproductive capacity of a male animal, which belongs to the technical field of animal feed and comprises the following components by weight percent: 6.5-8.5% of food grade vitamin E, 11.0-15.5% of food grade vitamin C, 20.0-40.0% of food grade tea polyphenols, 4.0-6.0% of food grade thioctic acid and the balance of food grade corncobpowder which is taken as a carrier. The composite antioxidant can significantly reduce the content of nitrogen oxide free radicals and malonaldehyde in serum, improve the activity of SOD enzyme, significantly increase the number of sperms and the number of a grade sperms (the sperms with strong activity) in a male rat and improve the reproductive capacity of the male animal.

The invention provides a Chinese herbal medicine fodder additive for accelerating crucians to grow and enhancing the immunity and a fodder containing the additive. The Chinese herbal medicine fodder additive is formed by drying malts, hawthorns, green teas, lotus leaves, radix ophiopogonis and semen cassiae, and then performing crushing and uniform mixing. The Chinese herbal medicine fodder additive is added into a common crucian fodder so that the growing speed of the crucians can be obviously improved, the water content of muscles is reduced, the protein content of the muscles is improved and the fat content of the muscles is reduced; the hepatosomatic index can be reduced, and the spleen index and the head-kidney index can be improved; the blood serum T-SOD (Total-Superoxide Dismutase)can be obviously improved and the content of MDA (Malondialdehyde), TG (Triglyceride) and TC (cholesterol) is obviously reduced; the Chinese herbal medicine fodder additive has the characteristics ofgreenness, naturalness, no drug resistance, no residues, diversified functions, consideration of growth and disease resistance and the like; and the Chinese herbal medicine fodder additive can greatly reduce the breeding cost of the crucians and is suitable for industrial production.

The invention discloses an anti-oxidation active extract of citrus peels as well as an extraction process and application thereof. The active extract of the citrus peels mainly contains a citrus flavonoid substance and pectic polysaccharide. The extraction process of the active extract of the citrus peels comprises the steps of preprocessing raw materials, refluxing and extracting by a dipping method, decompressing and concentrating and drying in vacuum. The invention takes the in-vitro oxidation resistance of the extract to combine with the extraction yield as indexes, adopts a response surface method to optimize the extraction process, has reasonable technological conditions and good effect stability and is suitable for industrialized production. The active extract of the citrus peels, which is obtained by extraction, has an obvious effect to a model mouse with senility caused by D-galactose and can lower the content of the MDA (malondialdehyde) of the plasma, the liver and the brain obviously; the activity of the SOD (superoxide dismutase) of the plasma, the liver and the brain is obviously enhanced; the activity of the GSH-Px (glutathione peroxidase) of the plasma and the liver is obviously enhanced; and the invention can be used for preparing an anti-oxidation medicine, an anti-oxidation food or an anti-oxidation health-care product.

A do-it yourself test kit as a diagnostic agent for the detection of malonyldiadeyde and other aldehydes in the urine, which are formed in the course of the lipid peroxidation process caused by the free radicals. Also disclosed is a process for the preparation of the test, including suggested formulations of antioxidants as body's protectors against oxidative stress to the cells and help to reduce the risk of developing diseases due to the attack of free radicals.

The invention provides a film-coating fresh-keeping technology which is used for soaking the fresh pleurotus nebrodensis in 0.5 - 1.5 percent chitosan solution for 30 seconds. Compared with a technology without any treatment, the shelf life of the pleurotus nebrodensis which is treated by the film-coating fresh-keeping technology by using the 0.5 - 1.5 percent of chitosan solution can be prolonged from 17 days to 19 days and the weight-loss ratio in a preserving process and the contents of malondialdehyde (MDA) are both very low, and difference is significant (P<0.05). In an enzyme activity which takes part in an aging process, the pleurotus nebrodensis which is film-coated by the 0.5 - 1.5 percent of chitosan maintains higher anti-oxidation enzyme activity, and meanwhile, the activities of polyphenol oxidase (PPO) and prolease are the lowest, which illustrates that the speeds of browning and protein degradation are the slowest. Furthermore, the pleurotus nebrodensis which is film-coated by the 0.5 - 1.5 percent of chitosan also acts well in the aspect of nutrient contents, thereof, we think that the pleurotus nebrodensis which is film-coated by the 0.5 - 1.5 percent of chitosan can effectively prolong the fresh-keeping time of the pleurotus nebrodensis and is a reasonable and effective fresh-keeping technology.

Oxidative stress, resulting from the generation of reactive oxygen species, contributes to the development of a multitude of age-related diseases. Current methods of assessing oxidative stress levels range from the detection of lipid peroxidation products, such as F₂-isoprostanes and malondialdehyde, to monitoring the redox status of glutathione. While useful, traditional biomarkers of oxidative stress are not without their drawbacks, including low in vitro concentrations and possible artifact formation. Disclosed herein are new marker compounds, including ascorbylated 4-hydroxy-2-nonenal, that are useful as biomarkers of oxidative stress.

The present invention relates to a composition comprising Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof as an effective ingredient.

Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the present invention is highly effective for the improvement of liver functions since it can lower the level of GOT, GPT, ALP, BUN and total bilirubin, which are major liver function indices. ALP and BUN are also used as kidney function indices, so the decrease of the level of ALP and BUN by the extract of the present invention indicates that Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the invention can improve kidney functions as well.

Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the present invention also can lower the amount of hydroxyproline in liver but increase the amount of hydroxyproline in kidney, suggesting that the extract above has excellent anti-fibrosis and kidney protecting effects.

In addition, Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the present invention can lower the level of malondialdehyde, an index of lipid peroxidation in liver and kidney tissues, suggesting that the extract has excellent anti-oxidative effect.

Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the present invention promotes cell viability in liver and kidney cell lines, indicating that the extract has excellent liver and kidney cell protective effects. Therefore, the composition of the present invention can be effectively used not only for anti-oxidation and anti-fibrosis but also for the protection and the improvement of liver and kidney functions.

The invention discloses a fluorescence probe used for malonaldehyde imaging inside living cells and a preparation method thereof and belongs to the technical field of biological fluorescence analysis. The fluorescence probe is obtained in the following way: benzoyl hydrazine (containing substituted benzoyl hydrazine) as the effective part of the probe and malonaldehyde and 1,8-naphthalimide as a fluorescence reporter group are connected through a certain linking group (such as imino group). The fluorescence probe can effectively detect malonaldehyde in a physiological environment, and endogenous and exogenous malonaldehyde bioimaging in living cells is successfully realized. Malonaldehyde bioimaging in living cells is realized for the first time in the invention. The optimal probe MDAP-1 has high sensitivity (the lowest detection limit reaches 0.6micron), strong fluorescence response and short response time, has excellent selectivity to malonaldehyde, but has no fluorescence response to metal ions, oxidation active products and active carbonyl compounds, etc.

The invention discloses preparation of a soybean anti-fatigue biological active peptide, belonging to an extraction processing technology of plant protein. The method comprises the following steps: adding water in high-denaturation soybean meal serving as a raw material to prepare a mixed solution; performing ultrasonic preprocessing on the mixed solution; adding neutral protease into the processed mixed solution to perform enzymolysis so as to obtain enzymatic hydrolysate; performing ultrafiltration treatment on the enzymatic hydrolysate by using a membrane separation technology after the enzymolysis to obtain biological active peptide with high peptide content and molecular weight of less than 5 kDa; then performing separation and purification by using anion exchange chromatography and reversed-phase high-performance liquid chromatography; and finally performing freeze drying to obtain the soybean anti-fatigue biological active peptide. The soybean anti-fatigue biological active peptide prepared by the preparation method disclosed by the invention has higher autioxidant enzyme activities and lower malondialdehyde (MDA) content after movement of a mouse; and results show that the soybean peptide has the functions of reducing cell injury caused by lipid oxidation to have an anti-fatigue effect by removing free radicals produced in the body and inhibiting the generation of lipid peroxides.

The invention relates to functional oligosaccharide having the growth promoting function of disease-resistant elicitor in cultivation of plant seedlings, in particular to application of chitosan oligosaccharide as the disease-resistant elicitor to cultivation of the plant seedlings. Chitosan oligosaccharide has the function of promoting seed germination, can enhance quality of the seedlings and promotes formation of strong seedlings. By measuring the content of malondialdehyde related to disease resistance, it is proved that chitosan oligosaccharide is an excellent disease-resistant inducing factor and can be prepared into chitosan oligosaccharide biopesticide to be used. Chitosan oligosaccharide serving as the main component can be combined with other components to be prepared into non-polluted pesticide, and further chitosan oligosaccharide derivative products are developed.

The invention relates to a method for preserving fresh walnut fruit, aiming at solving the problems that oxidation, rancidity, molding, sprouting, dehydration and the like of fresh walnut fruit are easy to occur when the fresh walnut fruit are preserved. The method mainly comprises the following steps: selecting complete fruits, soaking in calcium chloride in a refrigeration house, fumigating with thiabendazole, leaving the fruit to stand, sealing, packaging in vacuum, storing, delivering, and the like, thereby achieving preservation of the fresh walnut fruit. As the walnut fruit is sealed and packaged under an anaerobic condition, the green peel of walnuts can be prevented from being softened, the fresh walnuts are prevented from rot or metamorphism, the water content of the fresh walnut fruit is kept, the change velocities of grease acid values and peroxidation values are reduced, the generation of malondialdehyde is inhibited, the iodine value can be relatively well maintained, the good quality is maintained, the brown stain index of the green peel of the fresh walnut fruit after being preserved for 120 days is 0.13, the complete fruit rate is more than 95%, and the preserved fresh walnut fruit is all good in color, fragrance, taste and shape.

The invention relates to a gene for improving the stress resistance of a plant the nucleotide sequence of which is expressed in the SEQ ID NO: 1. The nucleotide sequence expressed by the SEQ ID NO:1 is inserted into a vector to obtain the recombinant plasmid of the invention; the vector can select a known eukaryotic expression in the field. The recombinant plasmid is converted into tobaccos to obtain a transgenic plant. Test shows that based on an MS culture medium additionally provided with NaCl, mannitol and ABA, the transgenic plant grows better than a wild plant. After drying the transgenic plant and the wild plant for 18 days, the transgenic plant grows better than the wild plant. The detecting data for the content of malondialdehyde shows that the damage degree of the transgenic plant under stress conditions is lower than that of the wild plant. Therefore, the gene for improving the stress resistance of a plant can be applied in the fields of anti-drought of the plant and improving the salt improvement.

The invention relates to a maca moisture mask with the oxidation-resistant effect and a preparation method. The maca moisture mask comprises konjac refined flour, a purple maca fresh root extract, a pyracantha fortuneana fruit extract, a ginkgo leaf extract, a white fungus extract and a luffa extract. According to the maca mask, the pure natural plant extracts are main components and can supplement rich oxidation-resistant factors for skin. The mask has the effects of delaying senescence and increasing skin elasticity, is soft in texture and good in skin attachment and can absorb and release a large number of nutritional ingredients. As known from mouse anti-aging experiments, the maca moisture mask can remarkably increase the content of hydroxyproline and superoxide dismutase and reduce the content of malondialdehyde and lipofuscin. By means of water retention and skin irritation experiments on skin of healthy females, it is known that the mask has good moisture retention, is free of irritation and has an ideal face beautifying effect, and anaphylaxis is not likely to happen.

The invention discloses a malondialdehyde fluorescence probe, and a preparation method and an application thereof. The structural formula of the probe is shown in the description. The synthesis method of the above probe molecule is simple, the probe realizes the detection of malondialdehyde in an aqueous solution, and the probe also can realize the detection of malondialdehyde in mammal cells or plant tissue environment through carrying out fluorescence imaging on human cervical cancer cells or onion tissues respectively co-incubated with a fluorescence probe FDP and malondialdehyde by a confocal microscope and observing the change of a green channel fluorescence signal.

The invention relates to a compound Chinese medicinal preparation with antifatigue and antioxidation effects, and a preparation method thereof. The compound Chinese medicinal preparation is characterized by comprising the following components in part by weight: 2 to 10 parts of manyflower solomonseal rhizome, 3 to 10 parts of astragalus, 1 to 5 parts of American ginseng, 1 to 3 parts of lucid ganoderma, 0.5 to 1.5 parts of cordyceps sinensis, and 0.5 to 1.5 parts of cornu cervi pantotrichum. According to a formula, the medicines can be prepared into various formulations. Through the administration experiment of aging mice and crowd, compound Chinese medicinal preparation oral liquid can obviously improve the content of superoxide dismutase (SOD) and glutathione (GSH-Px) of an organism, has an effect of resisting and removing free radicals of the organism and delays the aging process; and by observing time of swimming with a load attached to the body of mice, SOD and the content of malondialdehyde (MDA), large and middle doses of the Chinese medicinal preparation can prolong the time of swimming with the load attached to the body of mice, improve the SOD activity of serum, and reduce MDA value, so that the compound Chinese medicinal preparation can enhance the antifatigue and antioxidation effects of mice by resisting free radicals.

The invention discloses a rice chilling damage early identification and handling method. The rice chilling damage early identification and handling method comprises the steps that level assessment is conducted on rice chilling damage according to different malondialdehyde content, handling is carried out according to different chilling damage levels, and ATP aqueous solution is sprayed on blades of mild damage; ATP and alkaloid aqueous solution are sprayed on blades of moderate damage; ATP, alkaloid and sodium chloride solution are sprayed on blades of heavy damage, wherein ATP mainly supplements the nutrients needed for plant growth, and alkaloid and sodium chloride play the role of inhibiting malondialdehyde and reducing damage to plant organism.

The invention relates to traditional Chinese medicine composition with functions of relieving alcoholism and protecting the liver. The traditional Chinese medicine composition comprises raw medicinalmaterials as follows: flos puerariae, semen hoveniae, medicated leaven, fructus amomi rotundus, white poria cocos, radix ginseng, raw fructus crataegi, fructus schisandrae chinensis and raw oysters, and the raw medicinal materials are compatible and have the functions of eliminating dampness caused by alcohol and regulating qi-flowing for strengthening spleen. The pharmacological experiment indicates that with the adoption of the traditional Chinese medicine composition, drunkenness latency time of a mouse can be prolonged, drunkenness time of the mouse is shortened, content of alcohol in blood is reduced, level of ALT (alanine aminotransferase), AST (aspartate aminotransferase) and MDA (malondialdehyde) in the liver is reduced, activity of GSH-PX (glutathione peroxidase) and ADH (alcoholdehydrogenase) is improved, and the traditional Chinese medicine composition has excellent effects of relieving alcoholism and protecting the liver.

A biochemical marker aided culture technique for culturing the short-period cotton varieties 'Zhongmiansuo No.16, 24, 27 and 36' with prematurity but no presenility includes such steps as hybridization between premature but non-presenile parents, using SOD, POD and CAT as selection markers to choose the crossed descendants beginning from F2 generation, and conventional breeding.