15 results about "Malondialdehyde" patented technology

This invention belongs to the field of post-harvest quality testing technology for agricultural products, specifically relating to a method for predicting the degree of chilling injury in bitter gourd. The method uses *Bitter Gourd 'Du Ruan'* as the research object, employing segmented pre-cooling treatment to measure indicators such as chilling injury index, respiration rate, weight loss rate, firmness, chlorophyll content, malondialdehyde content, and antioxidant enzyme activity during storage. This determines suitable pre-cooling conditions, explores the impact of different pre-cooling settings on its physiological quality and chilling injury development during storage, and constructs a bitter gourd chilling injury prediction model based on key indicators. The method for predicting the degree of chilling injury in bitter gourd provided by this invention offers a theoretical basis and technical support for optimizing post-harvest cold chain processes for bitter gourd, mitigating low-temperature damage, and extending shelf life.

This invention discloses the application of the blueberry VcERF70 gene in improving cadmium tolerance in plants, belonging to the field of plant genetic engineering technology. The amino acid sequence of the VcERF70 protein is shown in SEQ ID NO.2, and the nucleotide sequence of its encoding gene is shown in SEQ ID NO.1. This invention constructs a plant overexpression vector containing the VcERF70 gene and genetically transforms it into blueberries. The resulting transgenic plants exhibit significantly reduced malondialdehyde and hydrogen peroxide content under cadmium stress, and significantly increased superoxide dismutase, catalase, peroxidase activities, and glutathione content. Experiments demonstrate that overexpression of the VcERF70 gene can effectively enhance the antioxidant defense capacity of blueberries, reduce cadmium stress-induced oxidative damage, and thus improve the blueberry's tolerance to cadmium. This invention provides a new gene resource and technical pathway for blueberry stress-resistant breeding.

The application provides a gene and an application thereof CaCA2 The application belongs to the technical field of gene breeding and relates to application of a gene in pepper resistance to low-temperature and weak-light stress. CaCA2 The application discloses a gene and an application thereof CaCA2 The transient overexpression of the gene can slow down wilting of pepper plants, improve net photosynthetic rate of pepper leaves, expression amount of antioxidant enzyme genes, soluble sugar, free proline and mineral element content, and simultaneously reduce intercellular CO2 concentration and malondialdehyde content. CaCA2 After the VIGS technology is used to transiently silence the gene, the plant height, stem diameter, biomass and root morphological parameters of the pepper are reduced in varying degrees, and the gene causes stoma to close, and the overall fluorescence intensity, photosynthetic performance index and trace element content are reduced. CaCA2 The transient overexpression of the gene in the pepper can relieve wilting of the pepper plants and improve the resistance to low-temperature and weak-light stress, and the resistance is weakened after the gene is silenced.

The application belongs to the field of plant genetic engineering, and specifically discloses application of Anemiospermum neriiforme AnWRKY38 gene in improving plant waterlogging stress resistance. The application constructs AnWRKY38 overexpression vector, and transforms Arabidopsis thaliana to obtain a transgenic line. Experimental results show that overexpression of AnWRKY38 gene can significantly improve the survival rate of plants under waterlogging stress, enhance photosynthetic activity, cell membrane integrity, antioxidant capacity and proline accumulation, and reduce malondialdehyde and hydrogen peroxide content. The application first excavates the new function of Anemiospermum neriiforme AnWRKY38 gene in improving plant waterlogging stress resistance, provides gene resources and effective technical means for plant waterlogging tolerance breeding, and can be used for waterlogging resistance genetic improvement of forest trees and ecological restoration plants, and has important application prospect.

This invention relates to the field of plant genetic engineering, specifically to the LbMYB75 gene of *Limonium bicolor* and its applications. This invention discloses that the transcription factor LbMYB75 is specifically highly expressed in the early stages of salt gland development and is significantly induced by salt. This invention confirms that the LbMYB75 gene positively regulates salt gland development and salt secretion in *Limonium bicolor*. Salt gland density is significantly increased in *Limonium bicolor* lines overexpressing the LbMYB75 gene, while salt gland density is significantly decreased in LbMYB75 mutant lines. The transcription factor LbMYB75 positively regulates the salt tolerance of *Limonium bicolor*; compared with the wild type, the overexpressing lines under high salt conditions show higher levels of malondialdehyde (MDA) and sodium. + and Na + / K + K decreased significantly. + Increased proline; This invention elucidates the regulatory role of the LbMYB75 transcription factor in salt gland development of *Limonium bicolor*, providing genetic resources and theoretical basis for improving the salt tolerance of non-halophytes through transgenic means, and contributing to the development and utilization of saline-alkali soils.

This invention relates to the field of grain chemical analysis technology, specifically a quality identification method for distinguishing between acutely oxidized corn and aged corn. Addressing the shortcomings of existing technologies that rely solely on single fatty acid values ​​to accurately differentiate the underlying deterioration mechanisms of corn, this method, under the premise that the test samples have similar fatty acid values, performs a multi-level comparison of core lipid oxidation metabolites by measuring free fatty acids, peroxide value, anisidine value, and malondialdehyde concentration in a stepwise manner; and extracts the effective components of the sample to measure free radical scavenging rate, analyzing the limit of damage to endogenous antioxidant capacity in cells. By calculating a unique quantitative comprehensive discrimination index from the above indicators, as well as multi-dimensional data such as sensory characteristics, the reverse scissor difference of macromolecular nutrients, and the specific response differences of targeted antioxidants, this invention overcomes the blind spot of misjudgment due to "same value, different quality," enabling accurate qualitative classification of deteriorated corn and guiding feed processing enterprises in scientific grading.

This invention belongs to the field of plant genetic engineering technology, specifically involving GhCHX6A The application of its encoded GhCHX6A protein in regulating cotton salt tolerance and / or cultivating salt-tolerant cotton. This invention discovers... GhCHX6A Expression levels were significantly upregulated under salt stress, and Na+ expression was also observed. + Transport activity. Through the action of cotton... GhCHX6A After gene silencing, the salt tolerance of cotton decreased significantly, specifically manifested as increased wilting, increased accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), decreased chlorophyll content, and reduced sodium content in cotton plant tissues. + / K + The balance has been disrupted. It is evident that... GhCHX6A Or its encoded GhCHX6A protein can regulate Na + and / or K + The transport of these substances helps maintain ion homeostasis to alleviate oxidative stress, providing technical support for the cultivation of salt-tolerant cotton varieties.

The application discloses a phospholipid-epicatechin liposome and a preparation method and application thereof, and relates to the field of food preservation technology. The preparation method of the phospholipid-epicatechin liposome comprises the following steps: taking a functional phospholipid fraction as a membrane material, taking epicatechin as an active component, and preparing the phospholipid-epicatechin liposome by an ethanol injection-ultrasonic method; and the functional phospholipid fraction is prepared by taking low-value sturgeon caviar as raw material, extracting by chloroform-methanol and purifying by silica gel column chromatography. The phospholipid-epicatechin liposome provided by the application can be applied to sturgeon caviar preservation, can effectively delay product brightness attenuation, inhibit abnormal accumulation of volatile basic nitrogen and malondialdehyde, retard excessive degradation of protein, and maximally retain key functional fatty acids such as EPA and DHA in the caviar.

The application discloses a method for improving the determination accuracy of the content of malondialdehyde in a biological sample, which comprises adding a buffer solution, a protease solution and a thiobarbituric acid (TBA) into a malondialdehyde standard solution, and determining the absorbance of the malondialdehyde standard solution; subsequently, a standard curve is constructed, and the actual concentration of the malondialdehyde in the biological sample is calculated through the measured absorbance of the biological sample solution. The sample processing process of the method is simple, the Buffer A buffer solution and the protease used can release the MDA combined with the protein, can not only avoid the adsorption interference of the protein precipitation on the red complex of the MDA and the TBA and the extraction limitation of the supernatant, but also can detect the protein combined state MDA, can significantly improve the detection limit of the sample, is good in selectivity, improves the accuracy of the detection result, and can be used for the trace detection of clinical precious samples.

This invention discloses a cryopreservation diluent containing fucoxanthin and its applications. Adding fucoxant to the cryopreservation diluent enhances the antioxidant capacity of frozen-thawed semen, reduces oxidative damage to goat sperm from reactive oxygen species during freezing-thawing, significantly improves sperm viability, motility, plasma membrane integrity, and total antioxidant capacity after freezing-thawing, enhances catalase and total superoxide dismutase activity, and reduces reactive oxygen species and malondialdehyde content. The effect is best when the fucoxant concentration is 2 μM during cryopreservation. The cryopreservation diluent of this invention is simple to prepare and operate, significantly improves the cryopreservation effect of semen, and has important practical application value.

This application discloses the application of the BnaCT3 gene and its SNP molecular markers in regulating the cold resistance of rapeseed. The nucleotide sequence of the BnaCT3 gene is shown in SEQ ID NO.1. Overexpression of the BnaCT3 gene in rapeseed significantly improves the survival rate of plants under low-temperature stress, reduces electrolyte leakage rate and malondialdehyde and hydrogen peroxide content, increases proline content and antioxidant enzyme activity, and enhances the cold resistance of rapeseed. Furthermore, this application identifies two SNP sites located in the promoter region of the BnaCT3 gene, whose specific base combinations constitute H1 and H2 allele combinations. Rapeseed materials carrying the H2 allele exhibit significantly better cold resistance than those carrying the H1 allele, and all currently promoted varieties lack the H2 allele. This application provides effective gene resources and molecular markers for the genetic improvement of rapeseed cold resistance, which can be used for overexpression transgenic breeding and marker-assisted selection breeding.

The application relates to the technical field of biological detection, in particular to a CNV marker related to oxidation resistance of Tibetan sheep PAK1 The CNV marker is located in a copy number variation of a candidate region chr21: 17939991~17941379 of a sheep reference genome Oar_v4.0 version PAK1 According to 2x2 ‑ΔΔCt Quantitative results, three types of copy number variations are divided: 2x2 ‑ΔΔCt =2 is normal type, 2x2 ‑ΔΔCt >2 is insertion type, 2x2 ‑ΔΔCt <2 is deletion type. The GSH-PX, SOD and NO levels of normal type and insertion type individuals are significantly higher than those of deletion type individuals; the level of malondialdehyde of the insertion type individuals is significantly lower than that of the deletion type individuals. The CNV marker has important application value for auxiliary breeding of Tibetan sheep.

This invention relates to the field of biomedical technology, providing the application of TrxR1 as a target in the prevention and treatment of PLTX toxicity damage. Experimental studies of this invention show that PLTX specifically targets TrxR1, inhibiting or weakening its biological activity. Simultaneously, through the cystine / GSH / GPx4 axis, it leads to a decrease in downstream GSH (reduced glutathione) synthesis, a reduction in glutathione peroxidase 4 (GPX4) activity, and promotes the accumulation of intracellular reactive oxygen species (ROS), Fe... 2+ Elevated levels of PLTX further accelerate lipid peroxidation (LPO), leading to the production of complex compounds such as malondialdehyde (MDA) that mediate ferroptosis. Overexpression of TrxR1, however, can inhibit ferroptosis and reverse PLTX-induced damage. This invention clarifies for the first time the specific molecular damage mechanism of PLTX, contributing to the development of treatment strategies targeting the toxins themselves at both the cellular and molecular levels, and unlocking their potential clinical application value.

This invention belongs to the field of genetic engineering technology, specifically relating to a CtVOZ1 protein, gene, and applications related to plant drought resistance. This invention provides a CtVOZ1 protein related to plant drought resistance, the amino acid sequence of which is shown in SEQ ID NO.1. The CtVOZ1 protein of this invention can improve plant drought resistance. Examples show that overexpression... CtVOZ1 Genes can increase plant biomass, seed germination rate, chlorophyll content, and maintain photosynthetic efficiency. Compared to wild-type plants, overexpression of these genes... CtVOZ1 Following gene modification, the accumulation of malondialdehyde, superoxide anion, and hydrogen peroxide can be reduced, and the activity of antioxidant enzymes can be significantly increased, thereby improving plant drought resistance. This invention... CtVOZ1 Proteins can yield new germplasm with drought-resistant properties, which is of great significance for breeding drought-resistant crops.

The present application relates to non-invasive physiological indicator detection and health risk assessment technical field, disclose a kind of based on malondialdehyde grading detection and interpretation method of urine sample.The method comprises: obtaining urine sample, make it and the preset malondialdehyde color reaction system occur color reaction, form color result in preset reaction time;After color reaction is stable, the color information of the color result is collected, and the corresponding color feature parameter is extracted;Based on the color feature parameter, call grading interpretation model to determine the level of malondialdehyde in urine, output corresponding grading detection result.The grading result is presented in the form of intervalization grade, for reflecting the physiological environment state related to oxidative stress.