117 results about "Malondialdehyde" patented technology

The invention belongs to the technical field of biology, and particularly relates to application of a PdbUNE12 gene in improving the salt tolerance of populus sutchuenensis and a method for improving the salt tolerance of populus sutchuenensis. The nucleotide sequence of the PdbUNE12 gene is as shown in SEQ ID NO. 1 (Sequence Identifier Number 1). The invention finds that the overexpressed PdbUNE12 gene can reduce the electrolyte permeability of a plant under salt stress, can reduce the malondialdehyde content of the plant under salt stress, and can improve the SOD enzyme activity of the plant under salt stress, so that the invention provides the application of the PdbUNE12 gene in improving the salt tolerance of populus sutchuenensis.

The invention provides an application of an active peptide in preparation of a medicine for preventing and treating ALD (Atomic Lymphosis Disease). The amino acid sequence of the active peptide is shown as SEQ ID NO: 1, the active peptide can effectively relieve damage of ethyl alcohol to liver cells, increase of glutamic-pyruvic transaminase activity, lactic dehydrogenase activity and malondialdehyde content and decrease of glutathione content caused by ethyl alcohol can be remarkably inhibited, then the preventing and treating effect on ALD is achieved, and the active peptide is suitable for preparing drugs for preventing and treating ALD and has a good application prospect. Meanwhile, an active peptide resource library with ALD prevention and treatment effects is also enriched.

The invention discloses a ferroptosis inhibitor and application of the ferroptosis inhibitor in radiation pneumonitis drugs, and relates to the technical field of biological medicines. The specific preparation method comprises the following steps: adding the ferroptosis inhibitor and the auxiliary agent into the solvent, and dissolving to obtain the ferroptosis inhibitor composition; wherein the ferroptosis inhibitor is isoorientin; the auxiliary agent is a glucosamine derivative; the solvent is a serum-free DMEM culture medium. The ferroptosis inhibitor composition prepared by the method provided by the invention can effectively reduce the rise of active oxygen, ferrous ions and malondialdehyde levels of human pulmonary epithelial cells BEAS-2b and mouse pulmonary epithelial cells MLE-12 caused by irradiation, restore cell mitochondrial membrane potential enrichment and inhibit the expression of various inflammatory factors in irradiated cells; therefore, the process of radiation pneumonitis is remarkably improved, and a new medication strategy is provided for research on radiation pneumonitis drugs.

The invention discloses a method for evaluating and screening salt tolerance of triticale germplasm resources in a germination stage, and belongs to the technical field of evaluation and screening of plant salt tolerance, salt stress concentration screening: triticale seeds are treated by NaCl solutions with different concentrations, indexes such as survival rate, plant height and overground part fresh weight are measured, the NaCl solution with the concentration of 200 mmol. L <-1 > is determined as the salt tolerance evaluation appropriate concentration, and the salt stress concentration is determined as the salt tolerance evaluation appropriate concentration. Salt tolerance evaluation test: carrying out salt stress treatment by adopting a 200mmol. L <-1 > NaCl solution, determining phenotypic indexes such as survival rate, plant height, fresh weight of overground part, root length and chlorophyll content, and physiological and biochemical indexes such as catalase, peroxidase and superoxide dismutase activity and malondialdehyde content, and carrying out principal component analysis, membership function analysis and clustering analysis to obtain a salt tolerance evaluation result. Calculating a salt tolerance comprehensive evaluation value, and screening salt tolerance triticale germplasm resources.

The invention discloses a high-flexibility PET decorative film and a preparation method thereof, and belongs to the technical field of PET decorative films. According to the high-flexibility PET decorative film, the self-made flame retardant and 1, 3-benzene-5-boric acid are pre-polymerized to obtain a prepolymer, then the prepolymer, ethylene glycol and terephthalic acid are mixed to react, and then (4-carboxyl-2-nitrophenyl) malonaldehyde is used for blocking to obtain flame-retardant PET. The preparation method comprises the following steps: carrying out a pre-mixing reaction on flame-retardant PET and a modified nucleating agent, internally mixing the pre-mixed material with an antioxidant and a lubricant, carrying out extrusion granulation, and carrying out melt extrusion, sheet casting, two-way stretching and heat setting by using a double-screw extruder to obtain a PET base film; and finally, plating aluminum on the PET base film. The self-made flame retardant is obtained by enabling amino siloxane and 9, 10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide to react with each other; the modified nucleating agent is obtained by modifying calcium carbonate with glycine; the high-flexibility PET decorative film prepared by the invention has good flexibility, flame retardance and antistatic property.

The invention provides a cold-resistant gene PbbZIP44 which is separated and cloned from a pyrus betulaefolia stock and contains a BRZL structural domain, and provides application of the gene in improvement of cold resistance of plants. An over-expression vector and a silent vector of the PbbZIP44 gene are constructed, and are introduced into arabidopsis thaliana, pyrus calluses and pyrus betulaefolia seedlings by using an agrobacterium tumefaciens-mediated genetic transformation method, so that stable expression and transient expression are realized, and a stable over-expression plant and a TRV-mediated silent strain are obtained and are used for low-temperature resistance function analysis. A research result shows that under a low-temperature stress condition, compared with a wild type plant, an overexpression strain shows a stronger growth state, lower malondialdehyde content and relative conductivity, and higher photosynthetic efficiency and oxidation resistance; and the silent strain shows an opposite physiological reaction.

The invention discloses application of polydatin in preparation of a medicine for relieving hepatotoxicity caused by zearalenone, and belongs to the technical field of biological medicine. The invention reveals that polydatin inhibits hepatotoxicity caused by zearalenone through targeted xanthine oxidase for the first time, so that the toxicity of zearalenone to an organism is relieved. Polydatin can be directly combined with xanthine oxidase to inhibit the catalytic activity of xanthine oxidase, so that the oxidative stress injury of the liver after ZEA exposure is reduced, meanwhile, the content of malondialdehyde (MDA) in the liver can be remarkably reduced, the activity of superoxide dismutase (SOD) is improved, the liver oxidation-antioxidant balance is recovered, and liver cells are protected from oxidative injury of ZEA. The invention provides a new scientific basis and development direction for application of polydatin in the fields of mycotoxin detoxification, oxidation resistance, liver protection and the like.

The invention discloses a banana drought-enduring functional gene MaPUB21 and application of the banana drought-enduring functional gene MaPUB21 in improvement of plant drought-enduring capability. A nucleotide sequence of the gene is shown as SEQ ID NO.2. The invention further discloses a method for preparing the banana drought-enduring functional gene MaPUB21. According to the invention, a silence vector (MaPUB21-pTRV) aiming at the MaPUB21 gene is constructed, and an agrobacterium-mediated method is utilized to carry out instantaneous silence in the Musa communis. Drought tolerance analysis shows that after drought stress treatment, compared with a wild type, the content of malonaldehyde, superoxide anions and hydrogen peroxide of the MaPUB21 silent strain is remarkably reduced, and the content of proline and the activity of peroxidase are remarkably improved. The result shows that the drought tolerance of the plant can be effectively improved by silencing the MaPUB21 gene. The gene provided by the invention provides a new resource for plant stress-resistant molecular breeding, and is of great significance for cultivating drought-resistant plants and improving the utilization rate of drought land.

The invention provides a cassava low-temperature-resistant MeSR34b gene. The CDS sequence of the cassava low-temperature-resistant MeSR34b gene is as shown in SEQ ID NO: 1. The cassava low-temperature-resistant MeSR34b gene is cloned from cassava for the first time, the expression of the MeSR34b gene in the cassava is inhibited under low-temperature stress, and research shows that the gene can remarkably improve the low-temperature resistance of the cassava, increase the malondialdehyde content, the proline cumulant and the like in the cassava, reduce oxidative damage of cells, yellow newborn leaves of the cassava and improve the yield of the cassava. The expression of various protein genes is regulated and controlled. The invention provides a new candidate gene for research on improvement of low temperature resistance, oxidative damage resistance and the like of plants.

The invention discloses a saline-alkaline tolerant bacterium strain and application thereof. The saline-alkaline tolerant bacteria strain is selected from any one or more of the following bacteria: Pseudoclavibacter sp., the preservation number of which is CGMCC No.32646, the preservation number of which is CGMCC No.32646, the preservation number of which is CGMCC No.32646, and the preservation number of which is CGMCC No.32646. The preservation number of the providencia sp. Is CGMCC (China General Microbiological Culture Collection Center) No.32647, and the preservation number of the providencia sp. Is CGMCC No.32647. The preservation number of the planococcus sp. Is CGMCC No.32648, the planococcus sp. Is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 15, 2024, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China. The bacillus amyloliquefaciens and the bacillus amyloliquefaciens have the capabilities of fixing nitrogen The saline-alkaline tolerant growth of plants can be obviously promoted, the content of chlorophyll and proline in rice is increased, the activity of antioxidant enzyme is improved, and the content of malondialdehyde is reduced; in addition, the providencia has nitrogen fixation capacity, the pseudoochrobactrum pseudococcum has iron production capacity, and the motility coccus has iron production and phosphorus solubilizing capacity.

This invention belongs to the field of post-harvest quality testing technology for agricultural products, specifically relating to a method for predicting the degree of chilling injury in bitter gourd. The method uses *Bitter Gourd 'Du Ruan'* as the research object, employing segmented pre-cooling treatment to measure indicators such as chilling injury index, respiration rate, weight loss rate, firmness, chlorophyll content, malondialdehyde content, and antioxidant enzyme activity during storage. This determines suitable pre-cooling conditions, explores the impact of different pre-cooling settings on its physiological quality and chilling injury development during storage, and constructs a bitter gourd chilling injury prediction model based on key indicators. The method for predicting the degree of chilling injury in bitter gourd provided by this invention offers a theoretical basis and technical support for optimizing post-harvest cold chain processes for bitter gourd, mitigating low-temperature damage, and extending shelf life.

The present invention belongs to the field of genetic engineering and specifically provides a protein PeuENH1 for improving plant salt tolerance. The amino acid sequence of the protein PeuENH1 is shown in SEQ ID NO.2. The protein PeuENH1 can improve plant tolerance to salt stress, reduce plant sensitivity to salt stress, participate in plant response to salt stress, reduce reactive oxygen content, H2O2 content and malondialdehyde content in transgenic plants under salt stress, increase proline content in transgenic plants, enhance the antioxidant enzyme system, reduce oxidative damage to the membrane system by reactive oxygen, thereby improving plant tolerance to salt stress. The protein has important theoretical and practical significance for improving saline-alkali land environment, increasing saline-alkali land utilization efficiency and expanding saline-alkali land vegetation coverage area.

The present invention belongs to the field of microbial technology, and particularly relates to a strain of Bifidobacterium animalis subsp. lactis, a product and its application in protecting the liver. The Bifidobacterium animalis subsp. lactis ( Bifidobacterium animalis subsp. lactis ) BB-35 provided by the present invention has a preservation number of CGMCC No. 27836. This strain can be used to reduce the expression of the senescence-related marker p21 in mouse hepatocytes, reduce the levels of senescence-related secretory phenotype factors, cystatin C levels and liver function-related biochemical indicators, thereby improving the liver senescence condition of mice and reducing the impact on other organs. In addition, this strain can also improve the oxidative stress indicators malondialdehyde and superoxide dismutase contents, and reduce the levels of inflammatory factors TNF-α and IL-6, providing technical support for the preparation of products for preventing, alleviating and / or treating liver senescence and liver injury.

The invention discloses application of metformin to improvement of crop seed germination and stress resistance, the metformin is used as a widely used hypoglycemic drug, has a cross-species conservative metabolism regulation function, can activate an antioxidant defense system of plants, maintain K < + > / Na < + > balance in the plants, and can improve the survivability of crops in saline-alkali soil. After the metformin is adopted to treat the seeds, the germination rate and the germination potential of the seeds are obviously improved, the chlorophyll content, the peroxidase content, the soluble protein content and the like of seedlings are obviously increased, the malondialdehyde content is obviously reduced, and the stress resistance is obviously enhanced. The technology is low in treatment cost, simple to operate and obvious in effect.

The application relates to the field of plant molecular breeding and low-temperature stress cultivation technology, and particularly relates to application of a CmoCK1 gene in creating a low-temperature-resistant rootstock of cucumber; through overexpression of the CmoCK1 gene and low-temperature treatment of a wild-type cucumber, the low-temperature resistance of the cucumber with overexpression of the CmoCK1 gene is significantly improved; the wild type and the overexpression plant are grafted as rootstocks to wild-type scions respectively, it is found that the cucumber with overexpression of the CmoCK1 gene as the rootstock can improve the low-temperature resistance of the scion cucumber, and the physiological indexes such as the relative conductivity and the malondialdehyde content of the above plants are determined, which shows that the new function of the CmoCK1 gene has good application potential, provides a gene resource and a new idea for reducing low-temperature stress, delaying the low-temperature response of the cucumber and further cultivating a low-temperature-resistant special rootstock of the cucumber in production, and has a wide application prospect and high use value.

The application provides application of a calcium sensing protein ShCBL1 gene and belongs to the technical field of biology.The application overexpresses or superexpresses the ShCBL1 gene in sugarcane or rice, so that the content of proline and superoxide dismutase in the sugarcane or rice is increased, the content of malondialdehyde is reduced, and the cold resistance is improved.Researches show that when the ShCBL1 is superexpressed or overexpressed in plants, the cold stress related physiological indexes such as proline, superoxide dismutase (SOD) and malondialdehyde (MDA) can be significantly affected, so that the cold resistance of the rice and the sugarcane is enhanced.ShCBL1 as a positive regulator of cold resistance regulation can be used for molecular breeding of cold resistance varieties.

This invention provides a cold-resistance gene containing a BRZL domain isolated and cloned from pear rootstock. PbbZIP44 This study also provides insights into the application of this gene in enhancing plant cold resistance. Through the construction of... PbbZIP44 Gene overexpression and silencing vectors were developed and introduced into Arabidopsis thaliana, *Pyrus pyrifolia* callus, and *Pyrus pyrifolia* seedlings using Agrobacterium-mediated genetic transformation. Stable and transient expression were achieved, resulting in stable overexpressing plants and TRV-mediated silencing lines for low-temperature resistance functional analysis. Results showed that under low-temperature stress, the overexpressing lines exhibited stronger growth, lower malondialdehyde content and relative conductivity, as well as higher photosynthetic efficiency and antioxidant capacity compared to wild-type plants; while the silencing lines showed the opposite physiological response.

The invention discloses a strain of spirillum harteri FtiPS-6 and an application of the strain of spirillum harteri FtiPS-6. The bacterial strain is classified and named as Herbaspiridium huttiense FtiPs-6, and the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No.37084. The invention further discloses a preparation method of the bacterial strain. The spirillum harteri FtiPS-6 disclosed by the invention can effectively promote the growth of buckwheat and improve the production capacity, so that the plant height, the fresh weight and the yield of a single plant are remarkably increased. The strain can also improve the tolerance of buckwheat to drought stress, so that the survival rate, the relative water content and the proline content of buckwheat are remarkably increased, the catalase activity level is improved, and the malondialdehyde content is reduced. In an in-vitro simulated drought culture environment, the proline production capability of the strain is induced to be improved. And the method has a relatively high application value.

This invention discloses the application of the blueberry VcERF70 gene in improving cadmium tolerance in plants, belonging to the field of plant genetic engineering technology. The amino acid sequence of the VcERF70 protein is shown in SEQ ID NO.2, and the nucleotide sequence of its encoding gene is shown in SEQ ID NO.1. This invention constructs a plant overexpression vector containing the VcERF70 gene and genetically transforms it into blueberries. The resulting transgenic plants exhibit significantly reduced malondialdehyde and hydrogen peroxide content under cadmium stress, and significantly increased superoxide dismutase, catalase, peroxidase activities, and glutathione content. Experiments demonstrate that overexpression of the VcERF70 gene can effectively enhance the antioxidant defense capacity of blueberries, reduce cadmium stress-induced oxidative damage, and thus improve the blueberry's tolerance to cadmium. This invention provides a new gene resource and technical pathway for blueberry stress-resistant breeding.

The application belongs to the technical field of feed additives, discloses a compound functional additive for relieving liver oxidative stress and endoplasmic reticulum stress of mandarin fish, and specifically discloses a feed additive which comprises the following components: sodium octanoate, sodium butyrate and rice husk powder. The additive provided by the application can effectively relieve liver oxidative stress and endoplasmic reticulum stress of mandarin fish, reduce the content of malondialdehyde, improve the ability of mandarin fish to resist oxidative enzyme activity, and is beneficial to the health of fish body.

The invention discloses a traditional Chinese medicine composition for treating ischemic diseases of lower limbs and also provides a preparation method of the traditional Chinese medicine composition, the synergistic combination effect of the two medicines is exerted, the curative effect of a single medicine is obviously improved, and the anticoagulant effect is far greater than that of the independent administration of caltrop; the sum of the action intensity of two independent administration is achieved, and the in-vitro anticoagulation effect and the effect of improving the ischemic blood flow of the lower limbs can be remarkably improved; blood vessel growth factors are remarkably increased, the activity of creatine kinase and lactic dehydrogenase is reduced, and tissue damage is relieved; glutathione peroxidase is promoted, malondialdehyde and active oxygen are inhibited, and the anti-oxidation effect is achieved; hypoxia and mitochondrial functions can be improved by inhibiting hypoxia-inducible factors (hypoxia-inducible factors)-1 alpha and mitochondrial compounds 3; the anti-inflammatory effect can be achieved by inhibiting CRP, IL-1beta and TNF-alpha; the rat lower limb ischemia can be obviously improved and treated.

The application discloses a method for detecting the freshness of large yellow croaker based on a Ti3C2Tx@CeO2 electrochemical sensor, and has the characteristics that the method comprises the following steps: placing the Ti3C2Tx@CeO2 electrochemical sensor in a large yellow croaker sample solution to be detected, testing the peak current at a xanthine characteristic peak of 0.75 V and the peak current at a malondialdehyde characteristic peak of 0.95 V by using a differential pulse voltammetry method, and calculating the concentration values of xanthine and malondialdehyde in the large yellow croaker sample to be detected according to the linear relationship between the xanthine concentration and the peak current at 0.75 V and the linear relationship between the malondialdehyde concentration and the peak current at 0.95 V, respectively, so that the method has the advantages of simultaneously detecting multiple freshness indexes, high synchronous detection capability, high sensitivity, low detection limit, simplicity and quickness, high specificity and high accuracy.

The application discloses a salt-tolerant bacillus subtilis OLN-19 and application thereof, and belongs to the technical field of microorganisms. The strain OLN-19 can effectively relieve the influence of salt stress on the pepper; the strain OLN-19 has the stress resistance and growth promotion effect, and the application verifies the stress resistance and growth promotion effect of the strain OLN-19 under 150 mmol L ‑1 NaCl solution, and it is found that the strain OLN-19 can still grow and has strong stress resistance and growth promotion effect; meanwhile, the strain OLN-19 can significantly improve the root activity, proline content and peroxidase activity of the pepper and reduce the malondialdehyde activity of the root. In conclusion, the application of the strain OLN-19 can provide a high-quality salt-tolerant strain for the salt resistance strengthening and growth promotion of the pepper, and lays a foundation for the yield increase and quality improvement and green production of the pepper.

The application provides a gene and an application thereof CaCA2 The application belongs to the technical field of gene breeding and relates to application of a gene in pepper resistance to low-temperature and weak-light stress. CaCA2 The application discloses a gene and an application thereof CaCA2 The transient overexpression of the gene can slow down wilting of pepper plants, improve net photosynthetic rate of pepper leaves, expression amount of antioxidant enzyme genes, soluble sugar, free proline and mineral element content, and simultaneously reduce intercellular CO2 concentration and malondialdehyde content. CaCA2 After the VIGS technology is used to transiently silence the gene, the plant height, stem diameter, biomass and root morphological parameters of the pepper are reduced in varying degrees, and the gene causes stoma to close, and the overall fluorescence intensity, photosynthetic performance index and trace element content are reduced. CaCA2 The transient overexpression of the gene in the pepper can relieve wilting of the pepper plants and improve the resistance to low-temperature and weak-light stress, and the resistance is weakened after the gene is silenced.

The invention relates to a vase solution for preserving cut lily flowers of an OT hybrid line and application of the vase solution. The vase solution takes water as a solvent and comprises salicylic acid, citric acid, calcium chloride, 6-benayl aminopurine, aspirin, ascorbic acid, silver thiosulfate and cane sugar. The vase solution can improve the ornamental quality of cut flowers, remarkably prolong the service life of flowering branches and delay turbidity of the vase solution; in addition, the soluble sugar content and peroxidase content of the petals can be increased, and the malondialdehyde content of the petals can be reduced. The vase solution provided by the invention has the advantages of low cost, and simple and easy preparation and use.

The invention relates to application of zeaxanthine in improving cold resistance of plants, and belongs to the technical field of agriculture. The invention provides an application of zeaxanthine in improving the cold resistance of a plant. The plant is dendrobium nobile. According to the method, zeaxanthine aqueous solutions with different concentrations are sprayed to the low-temperature-stressed dendrobium nobile, and by measuring various parameters of the dendrobium nobile, results show that the yellow leaf rate, the leaf falling rate and the relative electric conductivity of plants can be reduced by spraying the zeaxanthine aqueous solution with the concentration of 50 mg / L to the low-temperature-stressed dendrobium nobile plants, the low-temperature semi-lethal temperature of the plants is reduced, and the yield of the plants is increased. The content of malonaldehyde, soluble protein, soluble sugar, proline and chlorophyll in the plants can be increased, the stress resistance of the plants is enhanced, damage caused by low temperature is relieved, and it is comprehensively known that the cold resistance of the dendrobium nobile can be enhanced by externally applying the zeaxanthine aqueous solution with the concentration of 50 mg / L.

The present application belongs to the field of plant conservation, more particularly, relates to a composition for relieving waterlogging stress of Allium victorialis L. and application thereof. The composition of the present application is composed of matured cow dung and spermidine solution, the concentration of the spermidine solution is 0.1-1 mmol / L. Before planting Allium victorialis L., the matured cow dung is applied in the field, when the flood season comes and the plant height of Allium victorialis L. reaches about 60-80 cm, by using the conservation method of the composition, the harm of waterlogging stress on Allium victorialis L. can be continuously relieved, the content of proline in the leaf of Allium victorialis L. is increased, the content of malondialdehyde in the leaf of Allium victorialis L. is reduced, and the oxidative damage caused by waterlogging stress is reduced by increasing the activity of antioxidant enzyme in the leaf of the plant.

This invention provides a composition for alleviating intestinal oxidative stress damage and its application, the composition comprising 4'-galactosyllactose (4'-GL) and glycomacropeptide (GMP). This invention found that, compared with the individual concentrations of 4'-GL and GMP, the composition of 4'-GL and GMP more significantly promotes the release of superoxide dismutase and catalase, while also more significantly reducing the release of malondialdehyde, a marker of oxidative stress. The synergistic effect between the two allows 4'-GL and GMP to better alleviate intestinal damage in Drosophila. Simultaneously, the composition can improve the survival rate and climbing index of H2O2-induced Drosophila, prolong the intestinal length of diseased Drosophila, improve the integrity of the intestinal barrier, and induce a recovery trend in the Drosophila. This demonstrates that the composition has a mitigating effect on intestinal oxidative stress damage.

The application belongs to the field of plant genetic engineering, and specifically discloses application of Anemiospermum neriiforme AnWRKY38 gene in improving plant waterlogging stress resistance. The application constructs AnWRKY38 overexpression vector, and transforms Arabidopsis thaliana to obtain a transgenic line. Experimental results show that overexpression of AnWRKY38 gene can significantly improve the survival rate of plants under waterlogging stress, enhance photosynthetic activity, cell membrane integrity, antioxidant capacity and proline accumulation, and reduce malondialdehyde and hydrogen peroxide content. The application first excavates the new function of Anemiospermum neriiforme AnWRKY38 gene in improving plant waterlogging stress resistance, provides gene resources and effective technical means for plant waterlogging tolerance breeding, and can be used for waterlogging resistance genetic improvement of forest trees and ecological restoration plants, and has important application prospect.

The invention designs a new application of grape seed procyanidine in prevention and treatment of cataract. The method comprises the following steps: constructing a cataract injury model by inducing rabbit crystalline lens oxidative injury with hydrogen peroxide, culturing by adopting a domestic rabbit crystalline lens, detecting the activity of total superoxide dismutase, glutathione peroxidase and catalase and the content of malondialdehyde by observing the opacity and structural form of the crystalline lens, and detecting the expression of apoptosis regulation proteins BCL-2 and BAX in combination with immunohistochemistry. And the protective effect and molecular mechanism of the grape seed procyanidine on crystalline lens injury are researched. Researches show that the grape seed procyanidine has a protective effect on hydrogen peroxide induced rabbit crystalline lens injury, and the mechanism of the grape seed procyanidine is related to an antioxidant effect and adjustment of BCL-2 / BAX apoptotic protein; rabbit eye stimulation experiments prove that the grape seed procyanidine has no stimulation or only slight stimulation to rabbit eyes. Therefore, the grape seed procyanidine can prevent cataract, is a potential cataract treatment medicine, provides a new way for development of related medical products such as eye drops, and has good clinical application value.