76 results about "Malondialdehyde" patented technology

The invention discloses application of polydatin in preparation of a medicine for relieving hepatotoxicity caused by zearalenone, and belongs to the technical field of biological medicine. The invention reveals that polydatin inhibits hepatotoxicity caused by zearalenone through targeted xanthine oxidase for the first time, so that the toxicity of zearalenone to an organism is relieved. Polydatin can be directly combined with xanthine oxidase to inhibit the catalytic activity of xanthine oxidase, so that the oxidative stress injury of the liver after ZEA exposure is reduced, meanwhile, the content of malondialdehyde (MDA) in the liver can be remarkably reduced, the activity of superoxide dismutase (SOD) is improved, the liver oxidation-antioxidant balance is recovered, and liver cells are protected from oxidative injury of ZEA. The invention provides a new scientific basis and development direction for application of polydatin in the fields of mycotoxin detoxification, oxidation resistance, liver protection and the like.

The invention discloses a banana drought-enduring functional gene MaPUB21 and application of the banana drought-enduring functional gene MaPUB21 in improvement of plant drought-enduring capability. A nucleotide sequence of the gene is shown as SEQ ID NO.2. The invention further discloses a method for preparing the banana drought-enduring functional gene MaPUB21. According to the invention, a silence vector (MaPUB21-pTRV) aiming at the MaPUB21 gene is constructed, and an agrobacterium-mediated method is utilized to carry out instantaneous silence in the Musa communis. Drought tolerance analysis shows that after drought stress treatment, compared with a wild type, the content of malonaldehyde, superoxide anions and hydrogen peroxide of the MaPUB21 silent strain is remarkably reduced, and the content of proline and the activity of peroxidase are remarkably improved. The result shows that the drought tolerance of the plant can be effectively improved by silencing the MaPUB21 gene. The gene provided by the invention provides a new resource for plant stress-resistant molecular breeding, and is of great significance for cultivating drought-resistant plants and improving the utilization rate of drought land.

The invention provides a cassava low-temperature-resistant MeSR34b gene. The CDS sequence of the cassava low-temperature-resistant MeSR34b gene is as shown in SEQ ID NO: 1. The cassava low-temperature-resistant MeSR34b gene is cloned from cassava for the first time, the expression of the MeSR34b gene in the cassava is inhibited under low-temperature stress, and research shows that the gene can remarkably improve the low-temperature resistance of the cassava, increase the malondialdehyde content, the proline cumulant and the like in the cassava, reduce oxidative damage of cells, yellow newborn leaves of the cassava and improve the yield of the cassava. The expression of various protein genes is regulated and controlled. The invention provides a new candidate gene for research on improvement of low temperature resistance, oxidative damage resistance and the like of plants.

This invention belongs to the field of post-harvest quality testing technology for agricultural products, specifically relating to a method for predicting the degree of chilling injury in bitter gourd. The method uses *Bitter Gourd 'Du Ruan'* as the research object, employing segmented pre-cooling treatment to measure indicators such as chilling injury index, respiration rate, weight loss rate, firmness, chlorophyll content, malondialdehyde content, and antioxidant enzyme activity during storage. This determines suitable pre-cooling conditions, explores the impact of different pre-cooling settings on its physiological quality and chilling injury development during storage, and constructs a bitter gourd chilling injury prediction model based on key indicators. The method for predicting the degree of chilling injury in bitter gourd provided by this invention offers a theoretical basis and technical support for optimizing post-harvest cold chain processes for bitter gourd, mitigating low-temperature damage, and extending shelf life.

The application relates to the field of plant molecular breeding and low-temperature stress cultivation technology, and particularly relates to application of a CmoCK1 gene in creating a low-temperature-resistant rootstock of cucumber; through overexpression of the CmoCK1 gene and low-temperature treatment of a wild-type cucumber, the low-temperature resistance of the cucumber with overexpression of the CmoCK1 gene is significantly improved; the wild type and the overexpression plant are grafted as rootstocks to wild-type scions respectively, it is found that the cucumber with overexpression of the CmoCK1 gene as the rootstock can improve the low-temperature resistance of the scion cucumber, and the physiological indexes such as the relative conductivity and the malondialdehyde content of the above plants are determined, which shows that the new function of the CmoCK1 gene has good application potential, provides a gene resource and a new idea for reducing low-temperature stress, delaying the low-temperature response of the cucumber and further cultivating a low-temperature-resistant special rootstock of the cucumber in production, and has a wide application prospect and high use value.

The application provides application of a calcium sensing protein ShCBL1 gene and belongs to the technical field of biology.The application overexpresses or superexpresses the ShCBL1 gene in sugarcane or rice, so that the content of proline and superoxide dismutase in the sugarcane or rice is increased, the content of malondialdehyde is reduced, and the cold resistance is improved.Researches show that when the ShCBL1 is superexpressed or overexpressed in plants, the cold stress related physiological indexes such as proline, superoxide dismutase (SOD) and malondialdehyde (MDA) can be significantly affected, so that the cold resistance of the rice and the sugarcane is enhanced.ShCBL1 as a positive regulator of cold resistance regulation can be used for molecular breeding of cold resistance varieties.

This invention provides a cold-resistance gene containing a BRZL domain isolated and cloned from pear rootstock. PbbZIP44 This study also provides insights into the application of this gene in enhancing plant cold resistance. Through the construction of... PbbZIP44 Gene overexpression and silencing vectors were developed and introduced into Arabidopsis thaliana, *Pyrus pyrifolia* callus, and *Pyrus pyrifolia* seedlings using Agrobacterium-mediated genetic transformation. Stable and transient expression were achieved, resulting in stable overexpressing plants and TRV-mediated silencing lines for low-temperature resistance functional analysis. Results showed that under low-temperature stress, the overexpressing lines exhibited stronger growth, lower malondialdehyde content and relative conductivity, as well as higher photosynthetic efficiency and antioxidant capacity compared to wild-type plants; while the silencing lines showed the opposite physiological response.

The invention discloses a strain of spirillum harteri FtiPS-6 and an application of the strain of spirillum harteri FtiPS-6. The bacterial strain is classified and named as Herbaspiridium huttiense FtiPs-6, and the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No.37084. The invention further discloses a preparation method of the bacterial strain. The spirillum harteri FtiPS-6 disclosed by the invention can effectively promote the growth of buckwheat and improve the production capacity, so that the plant height, the fresh weight and the yield of a single plant are remarkably increased. The strain can also improve the tolerance of buckwheat to drought stress, so that the survival rate, the relative water content and the proline content of buckwheat are remarkably increased, the catalase activity level is improved, and the malondialdehyde content is reduced. In an in-vitro simulated drought culture environment, the proline production capability of the strain is induced to be improved. And the method has a relatively high application value.

This invention discloses the application of the blueberry VcERF70 gene in improving cadmium tolerance in plants, belonging to the field of plant genetic engineering technology. The amino acid sequence of the VcERF70 protein is shown in SEQ ID NO.2, and the nucleotide sequence of its encoding gene is shown in SEQ ID NO.1. This invention constructs a plant overexpression vector containing the VcERF70 gene and genetically transforms it into blueberries. The resulting transgenic plants exhibit significantly reduced malondialdehyde and hydrogen peroxide content under cadmium stress, and significantly increased superoxide dismutase, catalase, peroxidase activities, and glutathione content. Experiments demonstrate that overexpression of the VcERF70 gene can effectively enhance the antioxidant defense capacity of blueberries, reduce cadmium stress-induced oxidative damage, and thus improve the blueberry's tolerance to cadmium. This invention provides a new gene resource and technical pathway for blueberry stress-resistant breeding.

The application belongs to the technical field of feed additives, discloses a compound functional additive for relieving liver oxidative stress and endoplasmic reticulum stress of mandarin fish, and specifically discloses a feed additive which comprises the following components: sodium octanoate, sodium butyrate and rice husk powder. The additive provided by the application can effectively relieve liver oxidative stress and endoplasmic reticulum stress of mandarin fish, reduce the content of malondialdehyde, improve the ability of mandarin fish to resist oxidative enzyme activity, and is beneficial to the health of fish body.

The application discloses a method for detecting the freshness of large yellow croaker based on a Ti3C2Tx@CeO2 electrochemical sensor, and has the characteristics that the method comprises the following steps: placing the Ti3C2Tx@CeO2 electrochemical sensor in a large yellow croaker sample solution to be detected, testing the peak current at a xanthine characteristic peak of 0.75 V and the peak current at a malondialdehyde characteristic peak of 0.95 V by using a differential pulse voltammetry method, and calculating the concentration values of xanthine and malondialdehyde in the large yellow croaker sample to be detected according to the linear relationship between the xanthine concentration and the peak current at 0.75 V and the linear relationship between the malondialdehyde concentration and the peak current at 0.95 V, respectively, so that the method has the advantages of simultaneously detecting multiple freshness indexes, high synchronous detection capability, high sensitivity, low detection limit, simplicity and quickness, high specificity and high accuracy.

The application discloses a salt-tolerant bacillus subtilis OLN-19 and application thereof, and belongs to the technical field of microorganisms. The strain OLN-19 can effectively relieve the influence of salt stress on the pepper; the strain OLN-19 has the stress resistance and growth promotion effect, and the application verifies the stress resistance and growth promotion effect of the strain OLN-19 under 150 mmol L ‑1 NaCl solution, and it is found that the strain OLN-19 can still grow and has strong stress resistance and growth promotion effect; meanwhile, the strain OLN-19 can significantly improve the root activity, proline content and peroxidase activity of the pepper and reduce the malondialdehyde activity of the root. In conclusion, the application of the strain OLN-19 can provide a high-quality salt-tolerant strain for the salt resistance strengthening and growth promotion of the pepper, and lays a foundation for the yield increase and quality improvement and green production of the pepper.

The application provides a gene and an application thereof CaCA2 The application belongs to the technical field of gene breeding and relates to application of a gene in pepper resistance to low-temperature and weak-light stress. CaCA2 The application discloses a gene and an application thereof CaCA2 The transient overexpression of the gene can slow down wilting of pepper plants, improve net photosynthetic rate of pepper leaves, expression amount of antioxidant enzyme genes, soluble sugar, free proline and mineral element content, and simultaneously reduce intercellular CO2 concentration and malondialdehyde content. CaCA2 After the VIGS technology is used to transiently silence the gene, the plant height, stem diameter, biomass and root morphological parameters of the pepper are reduced in varying degrees, and the gene causes stoma to close, and the overall fluorescence intensity, photosynthetic performance index and trace element content are reduced. CaCA2 The transient overexpression of the gene in the pepper can relieve wilting of the pepper plants and improve the resistance to low-temperature and weak-light stress, and the resistance is weakened after the gene is silenced.

The invention relates to a vase solution for preserving cut lily flowers of an OT hybrid line and application of the vase solution. The vase solution takes water as a solvent and comprises salicylic acid, citric acid, calcium chloride, 6-benayl aminopurine, aspirin, ascorbic acid, silver thiosulfate and cane sugar. The vase solution can improve the ornamental quality of cut flowers, remarkably prolong the service life of flowering branches and delay turbidity of the vase solution; in addition, the soluble sugar content and peroxidase content of the petals can be increased, and the malondialdehyde content of the petals can be reduced. The vase solution provided by the invention has the advantages of low cost, and simple and easy preparation and use.

The invention relates to application of zeaxanthine in improving cold resistance of plants, and belongs to the technical field of agriculture. The invention provides an application of zeaxanthine in improving the cold resistance of a plant. The plant is dendrobium nobile. According to the method, zeaxanthine aqueous solutions with different concentrations are sprayed to the low-temperature-stressed dendrobium nobile, and by measuring various parameters of the dendrobium nobile, results show that the yellow leaf rate, the leaf falling rate and the relative electric conductivity of plants can be reduced by spraying the zeaxanthine aqueous solution with the concentration of 50 mg / L to the low-temperature-stressed dendrobium nobile plants, the low-temperature semi-lethal temperature of the plants is reduced, and the yield of the plants is increased. The content of malonaldehyde, soluble protein, soluble sugar, proline and chlorophyll in the plants can be increased, the stress resistance of the plants is enhanced, damage caused by low temperature is relieved, and it is comprehensively known that the cold resistance of the dendrobium nobile can be enhanced by externally applying the zeaxanthine aqueous solution with the concentration of 50 mg / L.

The present application belongs to the field of plant conservation, more particularly, relates to a composition for relieving waterlogging stress of Allium victorialis L. and application thereof. The composition of the present application is composed of matured cow dung and spermidine solution, the concentration of the spermidine solution is 0.1-1 mmol / L. Before planting Allium victorialis L., the matured cow dung is applied in the field, when the flood season comes and the plant height of Allium victorialis L. reaches about 60-80 cm, by using the conservation method of the composition, the harm of waterlogging stress on Allium victorialis L. can be continuously relieved, the content of proline in the leaf of Allium victorialis L. is increased, the content of malondialdehyde in the leaf of Allium victorialis L. is reduced, and the oxidative damage caused by waterlogging stress is reduced by increasing the activity of antioxidant enzyme in the leaf of the plant.

This invention provides a composition for alleviating intestinal oxidative stress damage and its application, the composition comprising 4'-galactosyllactose (4'-GL) and glycomacropeptide (GMP). This invention found that, compared with the individual concentrations of 4'-GL and GMP, the composition of 4'-GL and GMP more significantly promotes the release of superoxide dismutase and catalase, while also more significantly reducing the release of malondialdehyde, a marker of oxidative stress. The synergistic effect between the two allows 4'-GL and GMP to better alleviate intestinal damage in Drosophila. Simultaneously, the composition can improve the survival rate and climbing index of H2O2-induced Drosophila, prolong the intestinal length of diseased Drosophila, improve the integrity of the intestinal barrier, and induce a recovery trend in the Drosophila. This demonstrates that the composition has a mitigating effect on intestinal oxidative stress damage.

The application belongs to the field of plant genetic engineering, and specifically discloses application of Anemiospermum neriiforme AnWRKY38 gene in improving plant waterlogging stress resistance. The application constructs AnWRKY38 overexpression vector, and transforms Arabidopsis thaliana to obtain a transgenic line. Experimental results show that overexpression of AnWRKY38 gene can significantly improve the survival rate of plants under waterlogging stress, enhance photosynthetic activity, cell membrane integrity, antioxidant capacity and proline accumulation, and reduce malondialdehyde and hydrogen peroxide content. The application first excavates the new function of Anemiospermum neriiforme AnWRKY38 gene in improving plant waterlogging stress resistance, provides gene resources and effective technical means for plant waterlogging tolerance breeding, and can be used for waterlogging resistance genetic improvement of forest trees and ecological restoration plants, and has important application prospect.

The invention designs a new application of grape seed procyanidine in prevention and treatment of cataract. The method comprises the following steps: constructing a cataract injury model by inducing rabbit crystalline lens oxidative injury with hydrogen peroxide, culturing by adopting a domestic rabbit crystalline lens, detecting the activity of total superoxide dismutase, glutathione peroxidase and catalase and the content of malondialdehyde by observing the opacity and structural form of the crystalline lens, and detecting the expression of apoptosis regulation proteins BCL-2 and BAX in combination with immunohistochemistry. And the protective effect and molecular mechanism of the grape seed procyanidine on crystalline lens injury are researched. Researches show that the grape seed procyanidine has a protective effect on hydrogen peroxide induced rabbit crystalline lens injury, and the mechanism of the grape seed procyanidine is related to an antioxidant effect and adjustment of BCL-2 / BAX apoptotic protein; rabbit eye stimulation experiments prove that the grape seed procyanidine has no stimulation or only slight stimulation to rabbit eyes. Therefore, the grape seed procyanidine can prevent cataract, is a potential cataract treatment medicine, provides a new way for development of related medical products such as eye drops, and has good clinical application value.

The invention provides application of inhibiting expression of split protein in uranium-induced renal injury repair and treatment targets, the split protein comprises Drp1 and Fis1, and by inhibiting expression of the split protein Drp1 and Fis1, the concentration of glutathione and SOD enzyme activity after uranium-induced renal injury are improved, the concentration of malondialdehyde after uranium-induced renal injury is reduced, and the uranium-induced renal injury repair effect is improved. And the oxidative stress reaction caused by uranium can be recovered.

This invention relates to the field of plant genetic engineering, specifically to the LbMYB75 gene of *Limonium bicolor* and its applications. This invention discloses that the transcription factor LbMYB75 is specifically highly expressed in the early stages of salt gland development and is significantly induced by salt. This invention confirms that the LbMYB75 gene positively regulates salt gland development and salt secretion in *Limonium bicolor*. Salt gland density is significantly increased in *Limonium bicolor* lines overexpressing the LbMYB75 gene, while salt gland density is significantly decreased in LbMYB75 mutant lines. The transcription factor LbMYB75 positively regulates the salt tolerance of *Limonium bicolor*; compared with the wild type, the overexpressing lines under high salt conditions show higher levels of malondialdehyde (MDA) and sodium. + and Na + / K + K decreased significantly. + Increased proline; This invention elucidates the regulatory role of the LbMYB75 transcription factor in salt gland development of *Limonium bicolor*, providing genetic resources and theoretical basis for improving the salt tolerance of non-halophytes through transgenic means, and contributing to the development and utilization of saline-alkali soils.

The invention belongs to the field of medicines, and relates to a scutellarin aglycone derivative as well as a preparation method and application thereof. The scutellarin aglycone derivative has a structure as shown in a formula I. The preparation method comprises the following steps: reacting scutellarin (a compound II) with methanol to obtain a compound III, then reacting with bornyl chloroacetate (a compound IV) to obtain a compound V, and finally carrying out an alkaline hydrolysis reaction to obtain the compound I, the scutellarin aglycone derivative has the effect of preventing or treating cardiovascular and cerebrovascular related diseases, can improve the activity of superoxide dismutase (SOD), inhibit the production of lactic dehydrogenase (LDH) and reduce the malondialdehyde (MDA) level superior to that of scutellarin, has the inhibiting effect on inflammatory factors TNF-alpha, IL-6 and IL-1beta in cell supernatant superior to that of scutellarin, and can be used for preparing a medicine for treating cardiovascular and cerebrovascular related diseases, such as cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases and cardiovascular and cerebrovascular related diseases. The invention has the potential of preparing or preventing cardiovascular and cerebrovascular related diseases.

The invention relates to application of gastrodia elata fermentation liquor in preparation of a product for relieving chronic fatigue syndrome. Fresh gastrodia elata is extracted through a microbial fermentation technology to obtain gastrodia elata fermentation liquor, and the gastrodia elata fermentation liquor or the gastrodia elata fermentation liquor serves as a main raw material to form the product capable of relieving the chronic fatigue syndrome. Through a mouse chronic fatigue syndrome model, it can be known that the gastrodia elata fermentation liquor can reduce the content of lactic acid and urea nitrogen in mouse serum, enhance the activity of superoxide dismutase and glutathione peroxidase in liver tissue and reduce the content of malondialdehyde (MDA), and has a protection effect on brain tissue and gastrocnemius muscle tissue. The gastrodia elata fermentation liquor can improve the chronic fatigue syndrome by enhancing the oxidation resistance of the body and relieving oxidative stress injury, and can be applied to preparation of products for improving or treating the chronic fatigue syndrome.

This invention relates to the field of grain chemical analysis technology, specifically a quality identification method for distinguishing between acutely oxidized corn and aged corn. Addressing the shortcomings of existing technologies that rely solely on single fatty acid values ​​to accurately differentiate the underlying deterioration mechanisms of corn, this method, under the premise that the test samples have similar fatty acid values, performs a multi-level comparison of core lipid oxidation metabolites by measuring free fatty acids, peroxide value, anisidine value, and malondialdehyde concentration in a stepwise manner; and extracts the effective components of the sample to measure free radical scavenging rate, analyzing the limit of damage to endogenous antioxidant capacity in cells. By calculating a unique quantitative comprehensive discrimination index from the above indicators, as well as multi-dimensional data such as sensory characteristics, the reverse scissor difference of macromolecular nutrients, and the specific response differences of targeted antioxidants, this invention overcomes the blind spot of misjudgment due to "same value, different quality," enabling accurate qualitative classification of deteriorated corn and guiding feed processing enterprises in scientific grading.

The invention belongs to the technical field of plant peptide extraction, and particularly relates to a preparation method and application of chayote polypeptide. According to the preparation method of the chayote polypeptide provided by the invention, chayote in Yunnan, Zhejiang and Guizhou is taken as a raw material, an ultrasonic-assisted enzymolysis process is optimized, and the chayote polypeptide is prepared through ultrafiltration separation. The prepared chayote polypeptide is subjected to amino acid content detection, polypeptide sequence identification and cell activity detection. Experiments discover that the chayote polypeptide prepared by the invention has a remarkable protective effect on H2O2-induced oxidative damage, specifically, by activating an Nrf2 / NQO1 pathway, up-regulating the activity of antioxidant enzyme, reducing the levels of active oxygen and malondialdehyde and inhibiting apoptosis and inflammatory factor release, an anti-oxidation-anti-apoptosis-anti-inflammatory cell protection mechanism is shown, and the chayote polypeptide can be used for treating H2O2-induced oxidative damage. A scientific basis is provided for developing functional foods or medicines of the chayote polypeptide.

The application belongs to the field of molecular biology and genetic engineering technology, and particularly relates to application of Vitis amurensis VaWRKY72 gene in improving plant cold resistance. The application first finds that overexpression of the Vitis amurensis VaWRKY72 gene can regulate cold resistance of Arabidopsis and grape. Through overexpression of the Vitis amurensis VaWRKY72 gene in Arabidopsis and grape, it is found that the Vitis amurensis VaWRKY72 gene can reduce malondialdehyde content, reduce electrolyte permeability, increase proline content and activities of peroxidase, catalase and superoxide dismutase, remove excess ROS, and promote expression of cold stress related genes, thereby improving the tolerance of plants to low temperature stress.

The invention relates to the technical field of feed microorganisms, and particularly discloses bacillus licheniformis and application of the bacillus licheniformis in degradation of malondialdehyde in grease. The bacillus licheniformis PL-O-1-5 provided by the invention is classified and named as bacillus licheniformis, the bacillus licheniformis is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 26, 2025, and the preservation number is CGMCC No.36782. The bacillus licheniformis PL-O-1-5 can be used for preparing the bacillus licheniformis. The bacillus licheniformis PL-O-1-5 strain provided by the invention has excellent properties of acid resistance, salt resistance, sugar resistance and oil resistance, has very strong tolerance, and can be applied to various feed raw materials and feeds; through fermentation treatment, a certain effect on preventing rancidity of feed and raw material grease is achieved, meanwhile, the malondialdehyde value in rancidified feed can be reduced, and certain safety is achieved.

The invention relates to application of 6 '-O-caffeoyl arbutin in preparation of a medicine for treating dementia, and belongs to the technical field of biological medicine. The 6 '-O-caffeoyl arbutin disclosed by the invention has a remarkable dementia treatment value, the action mechanism accurately fits the core pathological process of dementia, a neuroprotective effect is played by relieving oxidative stress and inhibiting inflammatory reaction, oxidative stress related indexes such as superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) can be effectively adjusted, and the dementia treatment effect is improved. The release of lactic dehydrogenase (LDH) and malondialdehyde (MDA) and the level of damage-induced nitric oxide synthase (I NOS) are reduced, so that the NMDA-induced PC12 cell damage is protected.

This invention belongs to the field of plant genetic engineering technology, specifically involving GhCHX6A The application of its encoded GhCHX6A protein in regulating cotton salt tolerance and / or cultivating salt-tolerant cotton. This invention discovers... GhCHX6A Expression levels were significantly upregulated under salt stress, and Na+ expression was also observed. + Transport activity. Through the action of cotton... GhCHX6A After gene silencing, the salt tolerance of cotton decreased significantly, specifically manifested as increased wilting, increased accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), decreased chlorophyll content, and reduced sodium content in cotton plant tissues. + / K + The balance has been disrupted. It is evident that... GhCHX6A Or its encoded GhCHX6A protein can regulate Na + and / or K + The transport of these substances helps maintain ion homeostasis to alleviate oxidative stress, providing technical support for the cultivation of salt-tolerant cotton varieties.

The invention discloses application of a medicago sativa MsBAM41 gene (SEQ.ID.NO.1) in improvement of saline-alkali resistance and low-temperature composite stress resistance of plants, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the gene is shown as SEQ. ID. NO.1 (the full length is 1722 bp). The amino acid sequence of the protein coded by the medicago sativa MsBAM41 gene is as shown in SEQ.ID.NO. 2. According to the invention, MsBAM41-RNAi alfalfa is created, and the MsBAM41 expression of the MsBAM41-RNAi alfalfa is obviously reduced. Under composite stress, compared with a wild type plant and a transgenic plant, the wilting damage is heavier, the beta-amylase activity and the soluble sugar content are remarkably reduced, the electrolyte permeability and the malondialdehyde content are remarkably improved, and the membrane damage is aggravated. It is proved that MsBAM41 is crucial to resistance to composite stress, a key gene is provided for creating stress-tolerant medicago sativa by means of a genetic engineering technology, and the application prospect is wide.