31 results about "ABTS" patented technology

The invention relates to the technical field of analytical chemistry and food pharmacy detection, in particular to a rutin antioxidant activity colorimetric determination method based on a composite nano enzyme, which comprises the following steps: mixing a to-be-detected sample solution with an ABTS free radical cation working solution to carry out first reaction, so that an antioxidant substance in a sample and ABTS free radical cations are subjected to scavenging reaction; adding the composite nano enzyme, a 3, 3 ', 5, 5'-tetramethyl benzidine solution and a hydrogen peroxide solution into a system after the first reaction is completed, and initiating a second reaction; and measuring the absorbance value of the system after the second reaction under a specific wavelength, comparing the absorbance value with the standard curve obtained through the treatment in the same step, and calculating the antioxidant activity expressed by the equivalent concentration of rutin in the sample to be detected. By optimizing reaction conditions, the method provided by the invention simplifies operation steps and can provide accurate measurement results in a wider concentration range.

The invention belongs to the technical field of biology, and particularly relates to a novel antioxidant peptide and application thereof, and the amino acid sequence of the novel antioxidant peptide is shown as SEQ ID NO.1. The antioxidant peptide is high in biological activity, has relatively high scavenging capacity on DPPH. Free radicals and ABTS. Free radicals, has relatively high reducing capacity on Fe < 3 + >, and has wide application in the aspects of preparing antioxidant medicines, foods or cosmetics and the like. Meanwhile, the antioxidant peptide is short in sequence, easy to synthesize and suitable for large-scale production and application.

The invention discloses an eggshell membrane source antioxidant umami peptide and application thereof, the amino acid sequence of the eggshell membrane source umami peptide is at least one of CDDDF, CWDVYR and WHDCHR, and relates to the technical field of bioactive peptides. The eggshell membrane umami peptides with antioxidant activity are screened by using a method of combining virtual enzymolysis, machine learning, molecular docking and molecular dynamics simulation, and three eggshell membrane umami peptides are screened and can be combined with umami receptor T1R1 / T1R3 subunits, and the composite conformation is stable. Sensory evaluation and electronic tongue analysis prove that the eggshell membrane source antioxidant umami peptide has good umami characteristics, the umami threshold value is 0.04-0.10 mmol / L, the eggshell membrane source antioxidant umami peptide has DPPH free radical scavenging capacity, ABTS + free radical scavenging capacity and Fe < 3 + > reduction activity, a new raw material is provided for development of functional seasonings, and the eggshell membrane source antioxidant umami peptide has important application value.

The invention discloses an extraction process for optimizing general flavone of strobilanthes cusia through a response surface method. An ultrasonic extraction method is adopted to extract total flavonoids in strobilanthes cusia, on the basis of a single factor experiment, an extraction process of the strobilanthes cusia total flavonoids is optimized through a response surface method, and comprehensive experiment conditions, operability and rationality are considered, so that the optimal extraction process is obtained as follows: the material-liquid ratio is 1: 27, the ethanol concentration is 72%, the ultrasonic extraction time is 39 min, and the extraction time is 30 min; under the condition, the total flavone extraction rate reaches 0.736 + / -0.015% and is basically consistent with a response predicted value. The extraction process is simple and stable in operation and high in extraction rate. In-vitro anti-oxidation experiments show that the strobilanthes cusia total flavonoids have certain DPPH free radical and ABTS free radical scavenging capacity, and a reference basis is provided for more fully and reasonably utilizing strobilanthes cusia resources.

The invention discloses a pyrimidine derivative containing a thiosemicarbazide structure and application of the pyrimidine derivative, and relates to the technical field of medicines, the compound has good oxidation resistance and alpha-glucosidase resistance activity, and the compound is 2-(4, 4 '-difluorophenyl)-2-(4, 4'-difluorophenyl)-2-(4, 4 '-difluorophenyl)-2-(4, 4' The IC50 values of the compound 2-(4, 6-dimethyl pyrimidine-2-yl)-N-methylhydrazine-1-methylsulfamide on DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging activity and ABTS (2, 2, 6-dimethyl pyrimidine-2-yl)-N-methylhydrazine-1-methylsulfamide are 3.96 mu g / mL and 2.01 mu g / mL respectively, the IC50 value of the compound 2-(4, 6-dimethyl pyrimidine-2-yl)-N-methylhydrazine-1-methylsulfamide on alpha-glucosidase inhibitory activity is 38.62 mu g / mL, the effect is best, and the compound 2-(4, 6-dimethyl pyrimidine-2-yl)-N-methylhydrazine-1 The pyrimidine derivative containing the thiosemicarbazide structure has a general formula shown in the specification, and is superior to control medicaments such as vitamin C, vitamin E and acarbose.

This invention discloses a method for detecting antioxidants (AA) in solution using a redox sensor. Belonging to the field of detection applications, the method is as follows: An ABTS solution, a test solution containing AA, and a silver nanoparticle solution with oxidase-like activity are mixed thoroughly. An acidic solution is added to adjust the pH of the system, and the reaction is allowed to proceed for 2-3 hours. SERS detection is then performed under 532nm laser irradiation to measure the Raman peak value of the sample. AA is qualitatively detected by comparing the changes in the Raman peak value of the blank sample with that of the sample. This invention achieves accurate and inexpensive trace detection of AA by comparing the differences in the degree to which different concentrations of AA inhibit the oxidation of ABTS by oxidase-like enzymes with the reaction system without the addition of the antioxidant AA. The reaction system for detecting antioxidants explored in this invention, utilizing SERS technology for trace detection of the antioxidant AA, has significant implications for practical applications.

A novel polypeptide with double biological activities is obtained from sea cucumber intestines, and the sequence of the polypeptide is KADANNQWVDVK through high performance liquid chromatography purification and mass spectrometry analysis and identification. The in-vitro activity evaluation of a system shows that the polypeptide not only shows remarkable antioxidant capacity and can effectively remove ABTS free radicals, but also shows relatively strong pancreatic lipase inhibitory activity, and the IC50 value of the polypeptide reaches a remarkable level. The marine active peptide with double functions of oxidation resistance and metabolism regulation shows a good application prospect in the field of functional food and medicine development. The sea cucumber intestines are treated through an enzymolysis technology, low-molecular-weight polypeptides are separated through dialysis, de novo sequencing is carried out, and high-throughput polypeptides are synthesized. The polypeptide KADANNQWVDVK (lysine-alanine-aspartic acid-alanine-asparagine-asparagine-glutamine-tryptophan-valine-asparagine-valine-lysine) with the activity of resisting oxidation and inhibiting pancreatic lipase is screened, and the polypeptide KADANNQWVDVK (lysine-alanine-aspartic acid-alanine-asparagine-asparagine-asparagine-glutamine-tryptophan-valine-asparagine-valine-lysine) with the activity of resisting oxidation and inhibiting pancreatic lipase is verified. According to the method, sea cucumber processing byproducts are fully utilized, the resource utilization rate is increased, a scientific basis is provided for high-value utilization of sea cucumber intestines, and meanwhile, a potential material basis is provided for developing natural antioxidants and peptides with lipid-lowering functions.

The present application relates to the technical field of chemical preparations, in particular to a method for improving the bioavailability of astaxanthin in phaffia rhodozyma powder and application. The present application uses composite enzymes to pretreat phaffia rhodozyma powder, so that astaxanthin is fully released from the cells, and then specially-made eutectic solvent is used to efficiently extract the pretreated phaffia rhodozyma powder, and the extraction rate can reach more than 90%. The astaxanthin extract prepared by the present application has high antioxidant activity and high bioavailability, and the DPPH free radical scavenging rate can be up to 89.14%, the ABTS free radical scavenging rate can be up to 90.09%, and the bioavailability can be up to 81.41%. In addition, the present application is simple to operate, does not need ultrasonic, microwave and other assisted methods, has low requirements on equipment, can be suitable for industrial large-scale production, provides a feasible scheme for the green and efficient extraction of astaxanthin, and has a wide application prospect in the food, cosmetic and feed industries.

The invention discloses a polypeptide with antioxidant activity and application thereof, and belongs to the technical field of biological medicine and fine chemical engineering. The amino acid sequence of the polypeptide is selected from QY, YG, YYYY, QPYY or WHYHDYKY. HPLC and MS structure confirmation shows that the synthesized polypeptide is high in purity and accurate in structure. In-vitro chemical experiments prove that the polypeptide (especially WHYHDYKY) has extremely high ABTS and DPPH free radical scavenging capacity, and the activity of the polypeptide is superior to or equal to that of glutathione (GSH). Cell experiments show that part of polypeptides (such as QY, YG, YYYY and QPYY) can significantly improve the survival rate of HepG2 cells under an oxidative damage model, and have a significant cell protection function. The polypeptide can be applied to the fields of antioxidant drugs, functional food, cosmetics, industrial antioxidants and the like according to the characteristics of the polypeptide.

The invention discloses a fish-derived active peptide with antioxidant and hypoglycemic effects, a preparation and application, and belongs to the technical field of biological active peptides. The fish-derived active peptide with the effects of resisting oxidation and reducing blood sugar is at least one of peptide fragments with amino acid sequences as shown in SEQ ID NO: 1-SEQ ID NO: 3. According to the invention, three active peptides with antioxidant and hypoglycemic effects are extracted from salmon by-products by adopting protease enzymolysis, LC-MS / MS, a bioinformatics method and other methods, and the active peptides have high DPPH clearance rate, ABTS free radical clearance rate and alpha-glucosidase and alpha-amylase inhibition rate. Therefore, the compound has an important application prospect in the development of products with the effects of resisting oxidation and reducing blood sugar.

The invention discloses a polypeptide with antioxidant activity and application thereof, and belongs to the technical field of biological medicine and fine chemical engineering. The amino acid sequence of the polypeptide is selected from QY, YG, YYYY, QPYY or WHYHDYKY. HPLC and MS structure confirmation shows that the synthesized polypeptide is high in purity and accurate in structure. In-vitro chemical experiments prove that the polypeptide (especially WHYHDYKY) has extremely high ABTS and DPPH free radical scavenging capacity, and the activity of the polypeptide is superior to or equal to that of glutathione (GSH). Cell experiments show that part of polypeptides (such as QY, YG, YYYY and QPYY) can significantly improve the survival rate of HepG2 cells under an oxidative damage model, and have a significant cell protection function. The polypeptide can be applied to the fields of antioxidant drugs, functional food, cosmetics, industrial antioxidants and the like according to the characteristics of the polypeptide.

The invention discloses a hesperetin derivative containing a thiosemicarbazone structure and application of the hesperetin derivative, the molecular formula of the compound is C18H19N3O5S, the biological activity determination result shows that the compound has good anti-oxidation and anti-alpha-glucosidase activity, the IC50 value of DPPH scavenging activity is 6.17 mu g / mL, and the activity of the hesperetin derivative is equivalent to that of a control agent vitamin C. The hesperetin derivative containing the thiosemicarbazone structure and the application of the hesperetin derivative containing the thiosemicarbazone structure have good anti-oxidation and anti-alpha-glucosidase activity. The IC50 value of ABTS clearance activity is 0.04 mu g / mL, and the activity is improved by 200 times compared with that of a control medicament vitamin E; the IC50 value of the alpha-glucosidase inhibitory activity is 4.53 [mu] g / mL, which is improved by 51 times compared with the activity of a control agent acarbose; the structural formula of the hesperetin derivative containing the thiosemicarbazone structure is shown in the specification.

The application discloses a kind of polypeptide with antioxidant activity and application thereof, belong to biological medicine and fine chemical technology field.The amino acid sequence of the polypeptide is selected from QY, YG, YYYY, QPYY or WHYHDYKY.HPLC and MS structure confirmation, synthetic polypeptide is high in purity, accurate in structure.In vitro chemical experiment confirms that the polypeptide (especially WHYHDYKY) has very strong ABTS and DPPH free radical scavenging capacity, and the activity is better than or equivalent to glutathione (GSH).Cell experiment shows that part of polypeptide (such as QY, YG, YYYY, QPYY) can significantly improve the survival rate of HepG2 cell under oxidative damage model, and has significant cell protection function.The polypeptide of the application can be applied in the field of antioxidant drugs, functional food, cosmetics and industrial antioxidants, etc.

The invention discloses an enhanced colorimetric method for rapidly and selectively detecting glutathione and a kit thereof, and belongs to the technical field of biochemical detection. The method comprises the following steps: preparing a prussian blue solution, a Turnish blue solution, a ferrous chloride solution or a ferric chloride solution as a nano-enzyme catalysis solution; mixing the catalytic solution with an ABTS solution and a hydrogen peroxide solution according to a specific proportion to form a blank reaction solution; adding a glutathione-containing to-be-detected sample into the solution to carry out a chromogenic reaction; and determining the content of glutathione by determining the absorbance difference between the blank and the reaction solution to be detected in a specific wavelength range. The method is mainly used for rapid, selective and quantitative detection of glutathione in the fields of food, medicine and biological analysis.

The invention discloses a vanillic acid derivative containing a thiosemicarbazide structure and application of the vanillic acid derivative. The compound has relatively good oxidation resistance and alpha-glucosidase resistance activity; wherein the IC50 values of the compound 2-(4-(benzyloxy)-3-methoxybenzoyl)-N-isopropylhydrazine-1-methylsulfuryl on DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging activity, ABTS (2, 2 '-azinobenzoyl) scavenging activity and alpha-glucosidase inhibitory activity are respectively 5.33 mu g / mL, 5.84 mu g / mL and 34.99 mu g / mL, the effect is the best, and the IC50 values are superior to those of control medicaments such as vanillic acid, vitamin C, vitamin E and acarbose. The vanillic acid derivative containing the thiosemicarbazide structure has a general formula shown in the specification,

The invention belongs to the field of phytochemistry and medicines, and discloses an antioxidant active ingredient, namely, indole amide glycoside Portulaleramides A-U from Portulaleralea L., and a preparation method of the indole amide glycoside Portulaleramides A-U, wherein the indole amide glycoside Portulaleramides A-U is prepared from Portulalea L.. The invention also relates to a herba portulacae active component with antioxidant activity. According to the present invention, the indole amide glycoside Portulamides A-U can scavenge free radicals ABTS and DPPH, and the Portulamides N can reduce the level of intracellular reactive oxygen species (ROS) so as to provide a significant protection effect on t-BHP induced oxidative damage, such that the indole amide glycoside Portulamides A-U can provide significant protection effects for the t-BHP induced oxidative damage, and can provide significant protection effects for the t-BHP induced oxidative damage,

The invention provides a gastrodia elata grading method based on Q-marker and application of the gastrodia elata grading method, and belongs to the technical field of traditional Chinese medicine genuine medicinal material grading evaluation. The method comprises the following steps: firstly, determining six candidate compounds as gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C and parishin E; and verifying that the six candidate compounds are Q-markers with the antioxidant activity of the gastrodia elata by integrating multi-dimensional data such as contents of the six candidate compounds, in-vitro anti-free radical (ABTS) and in-vivo antioxidant (nematode H2O2 damage resistance) biological activity. According to the method, a Q-marker coefficient integrating candidate compound content, in-vitro chemical activity and in-vivo biological activity is constructed by using a radar map area, an integrated quantity-effect model for rapidly predicting the antioxidant activity of gastrodia elata through the Q-marker content is established, a result shows that a model predicted value is consistent with an experiment, and the score of high-quality gastrodia elata with good antioxidant and anti-aging activity is greater than or equal to 8.79.

This invention relates to a laccase, its mutants, and their applications, belonging to the field of biotechnology. The amino acid sequence of the laccase PnLac1 is shown in SEQ ID NO.2, and the nucleotide sequence is shown in SEQ ID NO.1. The mutants are site-directed mutations at positions 166 (aspartic acid), 348 (histidine), 403 (methionine), or 467 (leucine) of laccase PnLac1, with amino acid sequences shown in SEQ ID NO.4, 6, 8, and 10, and nucleotide sequences shown in SEQ ID NO.3, 5, 7, and 9. The laccase PnLac1 of this invention achieves a decolorization efficiency of 75% for crystal violet within 5 hours. When ABTS was used as a substrate, the enzyme activities of laccase mutants H348F and L467M increased by 1.89 times and 1.65 times, respectively, showing potential for degrading stubborn substances; when guaiacol was used as a substrate, the enzyme activities of laccase mutants D166A and M403F increased by 2.07 times and 3.72 times, respectively, showing high application prospects in the field of lignin.

The invention belongs to the technical field of food science and bioengineering, and provides a method for improving antioxidant and hypoglycemic activity of bovine bone enzymatic hydrolysate. The method comprises the following steps: performing ultrasonic pretreatment on cattle bone powder (500W, 30min), performing alkaline protease hydrolysis (50 DEG C, 5h), mixing the inactivated cattle bone powder with D-xylose (8: 1), and performing Maillard reaction (100 DEG C, 120min). The DPPH free radical scavenging rate and the ABTS free radical scavenging rate of the obtained product respectively reach 91% and 88.13%, and the antioxidant activity is remarkably improved; the synthetic peptide, such as FGFDGDFYR, has both the hydroxyl radical scavenging rate of 74.52% and the alpha-glucamylase inhibition rate of 70.2%, so that the double-function activity of resisting oxidation and reducing blood sugar is realized; meanwhile, the product is enhanced in palatable taste and reduced in bitter taste, is rich in meat flavor compounds (such as 2-methyl-3-furanmercaptan), has flavor and functional advantages, and promotes high-value utilization of bovine bones.

The present application relates to a kind of soybean polypeptide preparation method with antioxidant and antagonistic lead damage effect.The soybean polypeptide prepared in the present application has good DPPH free radical clearance rate, ABTS free radical clearance rate and Fe3+ reduction capacity in vitro;It can also alleviate the case that the body weight of mouse is slowly increased due to lead exposure, improve the learning and memory ability of lead exposure mouse, alleviate the organ damage of mouse caused by lead exposure, enhance the level of reduced glutathione, superoxide dismutase and catalase of organism, and alleviate the abnormal increase of malondialdehyde caused by lead exposure.The soybean polypeptide prepared in the present application has good in vitro antioxidant capacity, and has antagonistic effect on oxidative stress caused by lead exposure mouse, and can be used as non-toxic and harmless natural antioxidant.For the development of natural antioxidant food, it has far-reaching application prospect, and also provides a new feasible idea for the combined application of bioinformatics technology and traditional enzymatic technology.

The invention provides a preparation and screening method of royal jelly source antioxidant peptide based on in-vitro simulated digestion. The sequence of the antioxidant peptide is as shown in SEQ. ID. NO.1: FNYDPRY (recorded as P1). The method comprises the following steps: carrying out static in-vitro simulated digestion on MRJPs to obtain products at different digestion stages, carrying out polypeptide sequence identification by combining liquid chromatography-tandem mass spectrometry (LC-MS / MS), and further carrying out antioxidant activity prediction and safety evaluation on the identified polypeptide by utilizing bioinformatics, so as to obtain a plurality of candidate polypeptides; the candidate peptides are subjected to in-vitro antioxidant activity detection and comprehensive screening by adopting a DPPH method, an ABTS method and an FRAP method, and P1 in the screened candidate peptides is optimal under three in-vitro evaluation systems of the DPPH method, the ABTS method and the FRAP method. The antioxidant peptide can be used for preparing antioxidant functional food, health food, medicine or cosmetic compositions.

The invention relates to a high-throughput screening method for gadusol yield and application of the high-throughput screening method, and belongs to the technical field of detection. According to the method, firstly, the oxidation resistance of the gadusol is systematically and comprehensively represented through FRPI, ABTS and DPPH methods, the linear relation between the gadusol free radical scavenging rate or the ferric iron scavenging rate and the concentration is established through regression linear analysis and data fitting, and therefore the high-throughput detection method of the gadusol is established, then the method is applied to screening of whole genome target genes, and the detection accuracy of the gadusol is improved. And screening to obtain genes Zwf, Gnd, tkta, Gmha, Pyka, Fba, Pgk, TAL and Pfka which obviously influence the yield of the gadusol. According to the method, high-throughput screening of the yield of the gadusol is realized, and the method has a good practical application value.

The invention relates to the technical field of enzyme engineering, and provides an engineering bacterium for producing fungal laccase as well as a construction method and application of the engineering bacterium, and the engineering bacterium is a pichia pastoris engineering bacterium into which a fusarium fujikuroi laccase gene Lac-1 is introduced. The construction method comprises the following steps: cloning the laccase gene, and connecting the laccase gene to an expression vector pPICZ alpha A to obtain a recombinant vector pPICZ alpha A-Lac; and after being linearized, the recombinant vector is electrically transformed into pichia pastoris GS115 for heterologous expression. The invention also provides a separation and purification method of the laccase produced by the engineering bacterium, which comprises the steps of ammonium sulfate precipitation, ion exchange chromatography and gel filtration, and immobilization of the purified recombinant laccase by a covalent method. The method has the advantages that under the acidic condition, under the condition that ABTS (2, 2 '-nitrogen-bis (3-ethylbenzothiazole-6-sulfonic acid)) is used as a mediator, the efficient decolorization capacity is shown for various dyes (such as malachite green and crystal violet) with complex structures, the degradation rate within 24 hours reaches 90% or above, and the method has wide application prospects in the aspect of treating refractory acid dye wastewater.

The present application relates to the field of analytical chemistry and food and pharmaceutical testing technology, and particularly relates to a rutin antioxidant activity colorimetric determination method based on composite nano-enzyme, comprising: mixing a to-be-detected sample solution and an ABTS free radical cation working solution to perform a first reaction, so that antioxidant substances in the sample and ABTS free radical cations perform a scavenging reaction; adding the composite nano-enzyme, 3,3',5,5'-tetramethylbenzidine solution and hydrogen peroxide solution to the system after the first reaction is completed, to initiate a second reaction; measuring the absorbance value of the system at a specific wavelength after the second reaction, and comparing the absorbance value with a standard curve obtained through the same steps, to calculate the antioxidant activity in the to-be-detected sample in terms of the equivalent concentration of rutin; through optimization of the reaction conditions, the method of the present application simplifies the operation steps, and can provide accurate measurement results in a wider concentration range.

The invention discloses a green modification preparation method of AX with high viscosity and antioxidant activity, which comprises the following steps: adding EDC and NHS into an AX solution for esterification reaction, then adding cysteine for grafting, dialyzing and freeze-drying to obtain modified AX. According to the invention, Cys is successfully grafted to carboxyl of a side chain of AX through amidation reaction, so that thiolation modification of AX is successfully completed. Only 15.71%-18.75% of sulfydryl in the TAX subjected to thiolation modification is oxidized to generate a disulfide bond, and the biological activity of the sulfydryl is well reserved. Sulfydryl introduced through thiolation modification endows TAX with excellent oxidation resistance and adhesion property, and compared with TAX before modification, DPPH (1, 1-diphenyl-2-picrylhydrazyl) and ABTS (2, 2 '-azinobis (2, 3-azinobis (3, 4-azinobis (3, 4-azinobis (3, 4-azinobis (3, 4-azinobis (3,

This invention discloses a multifunctional peptide derived from bovine serum albumin, possessing antioxidant, hypoglycemic, and tyrosinase-inhibiting properties, as well as its preparation and applications, belonging to the fields of food, biopharmaceutical, and cosmetic technologies. This invention screened and verified a novel multifunctional peptide, SFLYEYSRR, with a defined sequence. SFLYEYSRR exhibits a maximum ABTS scavenging rate of 89.93%, a maximum DPPH free radical scavenging rate of 35.48%, a maximum hydroxyl free radical scavenging rate of 46.49%, an α-glucosidase inhibition rate of 64.36%, and a maximum tyrosinase inhibition activity of 48.33%, overcoming the limitations of existing single-functional peptides in application.

The present application belongs to the technical field of traditional Chinese medicine quality detection, and particularly relates to a method for detecting the antioxidant capacity of Radix Astragali broken wall decoction pieces based on HPLC-ABTS + The method is based on HPLC-ABTS + The analysis system is established, the regression equation of the Trolox concentration and the inverted peak area is established according to the inverted peak formed by the change of the ABTS response value after the reaction, the antioxidant capacity of the Radix Astragali broken wall decoction pieces is obtained through conversion, and the inverted peak area of each active index component is substituted into the regression equation to obtain the antioxidant capacity of each index component; the method can simply and intuitively reflect the strength of the antioxidant capacity of each active component and the total antioxidant capacity of the Radix Astragali broken wall decoction pieces, and then reflect the product quality from the antioxidant activity, and the automatic detection can be realized through the instrument setting, the operation is simple, safe and economical, and the method is suitable for mass quality detection of the Radix Astragali broken wall decoction pieces.

The application provides a total antioxidant status determination kit, comprising reagent R1 and reagent R2; the reagent R1 comprises: phosphate buffer 50-150 mmol / L, ABTS 1-100 mg / L, peroxidase 0.05-0.2 %, stabilizer 1-20 g / L, surfactant 0.01-1 %, preservative 0.01-0.1 %; the reagent R2 comprises: phosphate buffer 50-150 mmol / L, hydrogen peroxide 1-20 mmol / L, stabilizer 1-20 g / L, surfactant 0.01-1 %, preservative 0.01-0.1 %. The application further provides a preparation and use method of the kit. The kit in liquid two-reagent form can be widely applied to the analysis and detection of biochemical instruments, has high accuracy, good stability, safety and effectiveness, and meets the clinical detection and diagnosis requirements.

The invention discloses a carbon dot-coated phosphorus-aluminum zeolite room-temperature phosphorescent material as well as a preparation method and application thereof, and the preparation method comprises the following steps: taking a mixed aqueous solution of boric acid and ethylenediamine as a carbon dot precursor, taking aluminum-based zeolite as a confinement matrix, and synthesizing the carbon dot-coated phosphorus-aluminum zeolite through a blending heating method. Based on the long-life phosphorescent property of the material, when the material is used for time-resolved detection of 3-NT in urine of a diabetic patient, interference of short-life background fluorescence in a biological sample can be effectively avoided. Based on room-temperature phosphorescent induced TMB catalytic discoloration, colorimetric sensing of TAC in urine is realized, and compared with existing TAC detection technologies (such as DPPH, FRAP and ABTS methods), the TAC content in urine can be rapidly, simply, conveniently and visually detected. According to the dual-mode sensing strategy of 3-NT and TAC, simultaneous verification of multi-dimensional biomarkers in urine is realized, and a new way is provided for diabetes screening based on rapid and non-invasive urine analysis.

The invention discloses a rapid grinding preparation method of benzoic acid-nicotinamide eutectic and a new application of the benzoic acid-nicotinamide eutectic in the fields of antibiosis and antioxidation. The eutectic is prepared by adopting a liquid-assisted mechanical grinding method, specifically, benzoic acid and nicotinamide in a molar ratio of 1: 1 are firstly subjected to dry grinding, then acetonitrile in a specific proportion is added for wet grinding to form paste, and then the eutectic is obtained by drying. The method is green, rapid and efficient in process. The prepared co-crystal shows unexpected synergistic biological activity; the antibacterial effect of the co-crystal on escherichia coli and staphylococcus aureus is obviously superior to that of each component monomer and a physical mixture; meanwhile, in DPPH and ABTS free radical scavenging experiments, a synergistic anti-oxidation effect is also shown. The eutectic crystal can be used as a high-efficiency antibacterial agent and an antioxidant, and has a wide application prospect in the fields of food preservation and medicine.