4 results about "ABTS" patented technology

This invention discloses a method for detecting antioxidants (AA) in solution using a redox sensor. Belonging to the field of detection applications, the method is as follows: An ABTS solution, a test solution containing AA, and a silver nanoparticle solution with oxidase-like activity are mixed thoroughly. An acidic solution is added to adjust the pH of the system, and the reaction is allowed to proceed for 2-3 hours. SERS detection is then performed under 532nm laser irradiation to measure the Raman peak value of the sample. AA is qualitatively detected by comparing the changes in the Raman peak value of the blank sample with that of the sample. This invention achieves accurate and inexpensive trace detection of AA by comparing the differences in the degree to which different concentrations of AA inhibit the oxidation of ABTS by oxidase-like enzymes with the reaction system without the addition of the antioxidant AA. The reaction system for detecting antioxidants explored in this invention, utilizing SERS technology for trace detection of the antioxidant AA, has significant implications for practical applications.

The present application relates to the technical field of chemical preparations, in particular to a method for improving the bioavailability of astaxanthin in phaffia rhodozyma powder and application. The present application uses composite enzymes to pretreat phaffia rhodozyma powder, so that astaxanthin is fully released from the cells, and then specially-made eutectic solvent is used to efficiently extract the pretreated phaffia rhodozyma powder, and the extraction rate can reach more than 90%. The astaxanthin extract prepared by the present application has high antioxidant activity and high bioavailability, and the DPPH free radical scavenging rate can be up to 89.14%, the ABTS free radical scavenging rate can be up to 90.09%, and the bioavailability can be up to 81.41%. In addition, the present application is simple to operate, does not need ultrasonic, microwave and other assisted methods, has low requirements on equipment, can be suitable for industrial large-scale production, provides a feasible scheme for the green and efficient extraction of astaxanthin, and has a wide application prospect in the food, cosmetic and feed industries.

This invention discloses a multifunctional peptide derived from bovine serum albumin, possessing antioxidant, hypoglycemic, and tyrosinase-inhibiting properties, as well as its preparation and applications, belonging to the fields of food, biopharmaceutical, and cosmetic technologies. This invention screened and verified a novel multifunctional peptide, SFLYEYSRR, with a defined sequence. SFLYEYSRR exhibits a maximum ABTS scavenging rate of 89.93%, a maximum DPPH free radical scavenging rate of 35.48%, a maximum hydroxyl free radical scavenging rate of 46.49%, an α-glucosidase inhibition rate of 64.36%, and a maximum tyrosinase inhibition activity of 48.33%, overcoming the limitations of existing single-functional peptides in application.