The invention relates to the technical field of medicine, and particularly relates to a medical radiation protection spray and a preparation method and a use method thereof. The medical radiation protection spray is mainly prepared from hippophae rhamnoides, rosa roxburghii tratt, folium mori, buckwheat, radix angelicae pubescentis, aloes, ulva, lavender, chamomile, flores aurantii, strawflower, rose bud, menthol, olive oil and trihytdroxy methyl-aminomethane and by the steps of material cleaning, high temperature extraction, petroleum ether ultrasonic extraction, ethanol-chloroform microwaveextraction, reduced pressure concentration, blending and filtering to remove bacteria. The medical radiation protection spray can quickly penetrate into the skin, can clear away superoxide radicals with stronger force, repair damaged cell tissues, promote tissue regeneration and epithelial tissue healing, and moisturize and increase skin elasticity lastingly, not only has good anti-wrinkle and moisturizing as well as regeneration and repair functions, but also has multiple efficacies of invigorating the circulation of blood and dispersing blood stasis, resisting bacteria and eliminating inflammation, so as to remarkably reduce harm of rays to the skin, is high in cure rate and rapid in effect, and is free of side effect and high in safety.