Preparation method and application of deep-sea extracellular polymeric substances

A deep-sea bacteria and extracellular polysaccharide technology, applied in microorganism-based methods, biochemical equipment and methods, preparations for skin care, etc., can solve the problems of low degree of development and utilization, achieve good free radical scavenging ability, good moisture absorption Effect

Inactive Publication Date: 2012-10-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been some studies at home and abroad on the exopolysaccharides secreted by marine microorganisms, but overall the degree of development and utilization is still very low, especially in the field of cosmetics, there is no commercial marine bacterial exopolysaccharides

Method used

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  • Preparation method and application of deep-sea extracellular polymeric substances
  • Preparation method and application of deep-sea extracellular polymeric substances
  • Preparation method and application of deep-sea extracellular polymeric substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A preparation method of deep-sea bacterial exopolysaccharide, the steps are as follows:

[0040](1) Streak inoculate the purchased deep-sea bacteria (Zunongwangia profunda) SM-A87 strain on solid medium, activate and culture at 20°C for 3 days, then inoculate in 100mL liquid seed medium, and inoculate at 20°C, 200rpm Shake culture under conditions for 2 days, then inoculate 5L of fermentation medium at an inoculum size of 5% (v / v), culture for 24 hours at 20°C and dissolved oxygen saturation of 80-100%, and then adjust the fermentation temperature At 10°C, continue to cultivate for 9 days, and add 500 mL of lactose solution with a concentration of 200 g / L prepared by lactose and artificial seawater on the third and fifth days of continued cultivation to prepare a fermentation broth;

[0041] The above-mentioned solid medium components are as follows, all in parts by weight:

[0042] 1 part of peptone, 0.5 parts of yeast powder, 1.5 parts of agar, 100 parts of artificia...

Embodiment 2

[0049] Embodiment 2: Determination of moisture absorption and moisture retention performance of deep-sea bacterial exopolysaccharide

[0050] The specific method for the determination of the hygroscopicity of deep-sea bacterial exopolysaccharides is as follows:

[0051] The saturated ammonium sulfate solution and the saturated potassium carbonate solution were placed in two desiccators (300mm in diameter) respectively, and stored in a constant temperature box at 25°C to make environments with relative humidity of 81% and 43%, respectively.

[0052] Grind deep-sea bacterial exopolysaccharide, hyaluronic acid, chitosan oligosaccharide and sodium alginate to 80 mesh, put them in a vacuum desiccator together with glycerin, and use P 2 o 5 As a desiccant, dry at 40°C to constant weight.

[0053] Accurately weigh 0.5000g (accurate to 0.0001g) of deep-sea bacterial exopolysaccharide, hyaluronic acid, chitosan oligosaccharide and sodium alginate respectively, put them into 25×25mm w...

Embodiment 3

[0067] Determination of antioxidant properties of deep-sea bacterial exopolysaccharides, the specific method is as follows:

[0068] 1. Scavenging effect on organic free radicals (DPPH·)

[0069] The deep-sea bacterial exopolysaccharide deionized water was made into 0.1, 0.25, 0.5, 1.0, 2.0, 3.0, 5.0, 7.5 and 10.0 mg / mL solutions as sample groups. Vitamin C and hyaluronic acid were made into solutions of the same concentration with deionized water as a control group.

[0070] Dissolve DPPH with a small amount of absolute ethanol, and then use 50% ethanol to make a solution with a concentration of 100 μM.

[0071] Add 2mL of DPPH solution into a 10mL centrifuge tube, then add 1mL of sample solution, mix well, react at room temperature in the dark for 40min, and measure the absorbance Ai at 525nm.

[0072] Replace the sample with 1mL deionized water as the blank control tube, replace the DPPH solution with 2mL of 50% ethanol and add 1mL of polysaccharides with different concen...

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Abstract

The invention relates to a preparation method and application of deep sea extracellular polymeric substances. The preparation method comprises the following steps of: (1) inoculating a deep-sea bacterium SM-A87 stain to a liquid seed culture medium, carrying out shake culture for 2-3 days, then inoculating the stain to a fermentation culture medium for culturing for 20-30h, adjusting the fermentation temperature, and continuously culturing for 8-10 days to prepare a fermentation liquor; (2) adding absolute alcohol to the fermentation liquor for ethanol precipitation, separating to obtain a precipitate, washing the precipitate with absolute alcohol, and drying to obtain crude polysaccharide; (3) dissolving the crude polysaccharide into water, adding alkali protease for enzymolysis for 5-6h; and adding absolute alcohol for ethanol precipitation, separating to obtain a precipitate, washing the precipitate with absolute alcohol, and drying to prepare the deep-sea extracellular polymeric substances. The deep-sea extracellular polymeric substances prepared by the method have good moisture absorption and preservation properties and oxidation resistance and have a good application potential in the field of cosmetics.

Description

technical field [0001] The invention relates to a preparation method and application of deep-sea bacterial exopolysaccharide, belonging to the technical field of biotechnology. Background technique [0002] The rapid development and wide application of modern science and technology have brought new development opportunities to the cosmetics industry. Cosmetics have developed from basic skin care products for the purpose of cleansing and moisturizing to functional cosmetics for the purpose of delaying aging and beautifying skin tone. Cosmetics The research and development has gone through chemical beauty, plant beauty, and is developing towards the goal of biological beauty and gene beauty. The application of bioactive polysaccharides as functional cosmetic additives in cosmetics is an important direction of biological cosmetology. It can produce multiple functions such as moisturizing, delaying aging, promoting the proliferation of epithelial fibroblasts, and beautifying ski...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04A61K8/73A61Q19/00A61Q19/08C12R1/01
Inventor 张玉忠刘升波陈秀兰张熙颖解彬彬石梅秦启龙苏海楠宋晓妍高翔周百成
Owner SHANDONG UNIV
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