25000results about "Skin care" patented technology

A recombinant humanized collagen and a preparation method therefor. The recombinant humanized type III collagen can be efficiently and stably expressed in three expression systems of yeast, Escherichia coli, and CHO cells, and has the biological activity superior to that of a commercially available collagen product. The recombinant humanized type III collagen has structural characteristics and functional activity similar to those of a natural collagen, and thus has wide application prospects in biomedical materials, tissue engineering products, cosmetics, foods, health care products or drugs, medical instruments, medical cosmetology and the like.

The invention belongs to the technical field of cosmetics, and discloses anti-aging recombinant collagen as well as a preparation method and application thereof. The amino acid sequence of the anti-aging recombinant collagen is as shown in SEQ ID NO.1, and the nucleotide sequence of the anti-aging recombinant collagen is as shown in SEQ ID NO.2. The recombinant collagen CaI1 is constructed and expressed by recombining the effective sequences of the I-type collagen, the III-type collagen and the XVII-type collagen, and the recombinant collagen CaI1 is free of cytotoxicity, free of hidden danger of endotoxin and good in safety. The skin care product added with the recombinant collagen CaI1 can promote skin collagen absorption, inhibit skin aging and improve skin elasticity, and has a good application prospect.

The invention discloses an extraction and purification method and application of plant-derived exosome-like nanoparticles, and relates to the technical field of plant exosome preparation. The extraction and purification method comprises the following steps: washing fresh ginseng, airing for later use, adding a proper amount of buffer solution, mixing, breaking walls, filtering, centrifuging filtrate to obtain supernate, carrying out ultracentrifugation on the supernate to obtain ginseng exosome nano-particle precipitates, resuspending the precipitates, respectively adding pure water sucrose solutions with different concentrations, and carrying out ultracentrifugation to obtain ginseng exosome nano-particles; and re-suspending the target strip, and carrying out ultracentrifugation to remove redundant cane sugar so as to obtain the purified ginseng exosome-like nano-particles. The ginseng exosome-like nano-particles disclosed by the invention have a remarkable effect when being applied to cosmetics or medicines with skin photoaging resistance, skin oxidation resistance and wrinkle resistance. In an anti-UVB (Ultraviolet B) induced cell injury experiment, the ginseng exosome-like nano-particles have an excellent anti-UVB induced cell injury effect.

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention discloses a scalp essence capable of controlling oil and removing dandruff and a preparation method thereof, and provides the scalp essence capable of controlling oil and removing dandruff. The formula consists of the following raw materials: water, saccharomycetes / rice fermentation product filtrate, butanediol, phenoxyethanol, caprylic acid / capric triglyceride, galactose yeast-like bacterium fermentation product filtrate, allantoin, a saccharomycetes / zinc fermentation product, nicotinamide, arginine, polysorbate-20, hydrogenated lecithin, acrylic acid (ester) / C10-30 and an alkanol acrylate cross-linked polymer. Hydrolyzed sodium hyaluronate and trace components. According to the oil-controlling and dandruff-removing scalp essence disclosed by the invention, through the synergistic interaction among the raw materials, the effects of replenishing water, preserving moisture, controlling oil and removing dandruff are increased, the effects of relieving, recovering oil-water balance, recovering skin elasticity and delaying senescence are also achieved, the effects of preventing hair loss and nourishing hair are also achieved, and meanwhile, the oil-controlling and dandruff-removing scalp essence has the advantage of being stable in storage.

The invention provides humanized recombinant XVII collagen for promoting hair follicle stem cell repair and resisting aging as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The humanized recombinant XVII type collagen comprises polypeptide derived from the XVII type collagen or repeated protein formed by N times of repeated series connection of the polypeptide serving as a unit, n is an integer from 2 to 4; the polypeptide derived from the XVII type collagen comprises a polypeptide A and / or a polypeptide B; the amino acid sequence of the A polypeptide is as shown in SEQ ID NO: 1; and the amino acid sequence of the B polypeptide is as shown in SEQ ID NO: 2. The humanized recombinant XVII type collagen not only can be massively and stably recombined and expressed, but also has cell adhesion activity, cell proliferation promotion activity, cell migration promotion activity and excellent hair follicle repair and regeneration promotion activity, and has no sensitization side effect. The invention provides a new way for preparing collagen products with rich functions.

The invention relates to a recombinant A type humanized XVII type collagen as well as construction and application thereof, the protein is the humanized XVII type collagen which is composed of an intracellular region, a transmembrane region and an extracellular region, and the amino acid sequence is as shown in SEQ No. 1. The hair follicle repairing agent has the advantages of hair follicle repairing, hair regeneration, good biological activities of cell proliferation, cell adhesion and the like, and can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like. The gene sequence of the recombinant A type XVII collagen is 100% identical to the corresponding part of the gene sequence of the recombinant A type XVII collagen, so that the recombinant A type XVII collagen has good hydrophilicity and high activity, and has application potential in the fields of biomedical materials, beauty cosmetics, skin care products and the like.

The present application relates to the field of biotechnology. Provided are a PDRN, and a preparation method therefor and the use thereof. The PDRN has a GC content of 39%-42%. In the PDRN prepared by means of the method recited in the present application, the GC% in the gDNA thereof has a similarity of 98% or more to the GC% of human and is closer to the GC% in a human body than that of a salmon-derived PDRN. The PDRN of the present application has higher safety and efficacy, and compared with the extracting of PDRN from salmon semen in the prior art, the method for preparing the PDRN disclosed in the present application is simpler and more convenient.

To provide a mitochondria function-improving agent or the like which improves a mitochondria function in a skin cell.SOLUTION: A mitochondria function-improving agent that improves a mitochondria function in a cell contains components included in a swallow's nest as an active ingredient. According to each point of view of the present invention, a mitochondria function-improving agent or the like which improves mitochondria function in a skin cell can be provided.SELECTED DRAWING: Figure 3

The invention discloses a ginsenoside liposome. The ginsenoside liposome comprises ginsenoside, phospholipid and a surfactant, and the ginsenoside comprises protopanoxadiol or / and protopanaxatriol. The invention also discloses a preparation method of the ginsenoside liposome and application of the ginsenoside liposome in preparation of medicines, cosmetics or skin care products. According to the invention, the final desugared product of ginsenoside with the effect of treating skin toxicity reaction is prepared into the liposome, so that the membrane permeation and absorption capabilities of ginsenoside are greatly improved, the skin toxicity caused by tumor treatment can be quickly and effectively relieved, and the drug effect can be played for a long time; the invention provides a new method for treating skin toxic reaction caused by tumor treatment, and makes up for the requirements which cannot be met by the existing treatment means.

The invention discloses a calendula double-bacterium fermentation product as well as a preparation method and application thereof, and relates to the technical field of plant fermentation. The preparation method of the calendula double-bacterium fermentation product disclosed by the invention comprises the following steps: carrying out primary fermentation by using lactobacillus plantarum, then carrying out secondary fermentation by using saccharomyces cerevisiae, and carrying out sterilization and filtration treatment on the calendula fermentation liquid subjected to secondary fermentation, so as to obtain the calendula fermentation liquid. The calendula fermentation liquor is subjected to combined fermentation through lactobacillus plantarum and saccharomyces cerevisiae, and the content of polyphenol and flavone in the calendula double-bacterium fermentation product is remarkably increased. Meanwhile, the calendula double-bacterium fermentation product has the effects of preserving moisture, resisting oxidation, relieving and controlling oil, and has a wide application prospect in the field of cosmetics.

The invention provides an antibacterial peptide cAMP048 sourced from microorganisms in a deep sea environment and application of the antibacterial peptide cAMP048, and belongs to the technical field of peptide inhibitors. The antibacterial peptide cAMP048 has an amino acid sequence as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The antibacterial peptide can inhibit the growth of acinetobacter baumannii at 64 mu M, inhibit the growth of multi-drug-resistant acinetobacter baumannii and multi-drug-resistant enterococcus faecium at 128 mu M and inhibit the growth of escherichia coli, staphylococcus aureus and multi-drug-resistant klebsiella pneumoniae at 256 mu M. The antibacterial peptide can be used for preparing products with antibacterial and / or bactericidal performance, and can be applied to preparation of antibacterial and / or bactericidal products. Such as food, skin care products or medical supplies.

The invention discloses a cosmetic composition and a preparation method thereof, and belongs to the technical field of cosmetics. The invention discloses a chamomile essential oil toner which is mainly prepared from the following raw material components in parts by mass: 10 to 20 parts of modified toner, 50 to 60 parts of water, 4 to 6 parts of glycerol, 2 to 4 parts of propylene glycol, 0.8 to 1.2 parts of sodium chloride, 6.5625 to 7.875 parts of chamomile essential oil polymer micelle, 2.1875 to 2.625 parts of adhesive, 10.5 to 11.5 parts of capric triglyceride and 0.8 to 1.2 parts of cetyl polyethylene glycol. Through the synergistic effect of the modified toner and chamomile essential oil polymer micelles in a specific ratio, the dispersion stability of the toner is improved, meanwhile, the slow release effect of active ingredients is enhanced, and the chamomile essential oil toner has the advantages that the makeup holding property, the storage stability and the mildness are improved, meanwhile, the toner dispersity is improved, and the sensitive skin stimulation risk is reduced.

The invention relates to a barrier repair composition for activating cell energy and application thereof, the composition comprises the following components: nicotinamide, a wheat germ extract, oat beta-glucan and a golden camellia flower extract, the four components synergistically form a full-link barrier repair system of energy supply (nicotinamide)-autophagy cleaning (wheat germ extract)-structure strengthening (beta-glucan)-oxidation defense (camellia nitidissima extract), the limitation that a traditional skin care product singly supplements lipid or resists oxidation is broken through, lipid dynamic balance is rebuilt from the root of cell metabolism, and the skin care product has the advantages that the skin care effect is good; and certain skin smoothing and anti-aging effects are achieved.

The invention relates to a stable silk fibroin liquid condensation body, gel and a preparation method and application thereof, and belongs to the technical field of silk fibroin. The preparation method comprises the following steps: S1, dissolving silk fibroin in a lithium bromide solution, and then adding trypsin for incubation to obtain an enzyme-digested silk fibroin solution; and S2, performing low-temperature programmed centrifugal treatment including an induction stage, a nucleation stage and a liquid-liquid phase separation stage on the enzyme-digested silk fibroin solution, and cleaning to obtain the stable silk fibroin liquid-state aggregate, the method can reduce aggregate liquid drop aggregation and improve the particle size uniformity of the liquid drops.

According to the preparation method of the Huangshan pine needle polyphenol and the acne-removing and inflammation-diminishing essence, the Huangshan pine needle polyphenol is prepared by adopting a microbial solid fermentation method, release of soluble polyphenol substances in Huangshan pine needles is remarkably promoted, and compared with an unfermented group, the polyphenol content of pine needles reaches up to 146.32 + / -2.53 mg / g and is increased by 1.89 times. The pine needle polyphenol component is prepared by adopting ultrasonic-assisted deep eutectic solvent extraction, membrane filtration and separation and purification technologies, the method has the advantages of short extraction time, low extraction temperature, environmental friendliness and the like, the structural integrity of pine needle polyphenol is maintained to the greatest extent, and the antibacterial activity of polyphenol is remarkably improved. The acne-removing and inflammation-diminishing essence containing the Huangshan pine needle polyphenol is soft in formula and high in safety, has remarkable dual effects of moisturizing and acne removing, can effectively inhibit skin inflammation, promote repair of acne skin, maintain the health state of the skin and meet the requirements of people, and can be widely applied to the field of skin care products.

The invention relates to the technical field of cosmetics, in particular to an acne-removing composition, lipidosome and a preparation method and application thereof. The acne-removing composition disclosed by the invention is prepared from the following components in parts by weight: 2 to 8 parts of nicotinamide, 1 to 5 parts of cortex magnoliae officinalis extract, 0.15 to 2.01 parts of glycyrrhizic acid, 0.1 to 2 parts of totarol and 0.01 to 0.15 part of hyssopus officinalis extract. The nano-carrier is adopted to wrap and deliver the acne removal composition, so that the solubility of the functional components is increased, the bioavailability of the functional components is improved, meanwhile, the irritation of the functional components is also improved, multi-target co-delivery and multi-mechanism acne removal are realized, and the acne removal composition has a remarkable acne removal effect.

The invention relates to a composition containing camellia nitidissima extract and capable of brightening skin color and application of the composition to preparation of cosmetics suitable for dry sensitive skin, and the composition comprises the following components: camellia nitidissima flower extract, elm bark extract, oat kernel protein and laver polysaccharide in front of water temple, the weight ratio of the camellia nitidissima flower extract to the elm barks extract to the oat kernel protein to the laver polysaccharide in front of water temple is (1-10): (1-8): (0.1-1): (0.01-0.1). The composition can achieve the optimal long-acting brightening, barrier repairing and stimulation relieving effects on the aspect of efficacy.

The invention belongs to the technical field of protein engineering and biology, and discloses a recombinant III-type humanized collagen, a coding gene thereof, a protein preparation, a preparation method of the protein preparation, application of the protein preparation and a skin care product. The amino acid sequence of the recombinant III type humanized collagen is as shown in SEQ ID NO. 1. According to the recombinant III type humanized collagen, the transdermal absorption efficiency is greatly improved, and meanwhile, the proliferation and migration capabilities of keratinocytes are also remarkably improved. The collagen is widely applied to the fields of medical cosmetic plastic surgery, cosmetics and the like, and has a good application prospect.

The invention belongs to the technical field of genetic engineering, and particularly relates to an antibacterial peptide with antibacterial and antiviral effects and application and a product thereof. The amino acid sequence of the antibacterial peptide provided by the invention has the following general formula: X1GX2FX3FX4KKFX5LFX6X7SKVLK, x1 is M or has no amino acid; x2 is one of R, G and H; x3 is one of KS, KR, HR and HH; x4 is R or L; x5 is one of KK, HH and HK; x6 is RR or KK; and X7 is I or L. The antibacterial peptide disclosed by the invention has a relatively good antibacterial effect, and shows antiviral activity on HPV16 (Human Papilloma Virus 16) and HPV18 (Human Papilloma Virus 18). Good application prospects are shown in the preparation of antibacterial or antiviral products.

Disclosed are a pentapeptide compound and a composition and use thereof, relating to the technical field of polypeptides, the peptide or a salt thereof having a structure as shown in formula (I): R1-Lys-Lys-Leu-Ala-R2 (I). The invention also relates to a cyclic peptide, and the structure of the cyclic peptide is Cyclo-[Lys-Lys-Lys-Leu-Ala]. The compound disclosed by the invention has the effects of controlling oil, repairing, resisting oxidation and the like, and can be used for nursing skin or mucosa.

The invention discloses an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide with an inhibition effect on various clinically common pathogenic microorganisms is obtained, and the antibacterial peptide comprises gram-negative bacteria such as escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, vibrio parahaemolyticus, salmonella gallinarum, salmonella typhimurium and enterobacter sakazakii, and gram-positive bacteria. The antibacterial peptide provided by the invention can be used as an antibacterial peptide, such as staphylococcus aureus, listeria monocytogenes and bacillus cereus, and the antibacterial peptide provided by the invention is novel in structure and easy to prepare.

The invention discloses a ginseng exosome, an extraction method thereof and application of the ginseng exosome in preparation of anti-photoaging skin care products, and belongs to the technical field of biological medicines. In order to solve the technical blank of ginseng exosomes in the aspect of light aging resistance in the prior art and solve the technical problems of complicated steps, long time consumption and small treatment capacity of exosome extraction by an ultracentrifugation method in the prior art, the invention provides an extraction method of ginseng exosomes. Therefore, the extraction rate of the ginseng exosome is improved, the treatment steps are simplified, the time is saved, and the purpose of producing the ginseng exosome in a large scale is achieved. The ginseng exosome obtained through the extraction method can be applied to preparation of anti-photoaging skin care products, and clinical application of the exosome is achieved.

The invention discloses an extraction preparation method and application of algae micromolecule PDRN, and relates to the technical field of biology. The invention establishes a process for extracting PDRN from microalgae, which comprises the following steps: carrying out cell disruption on microalgae liquid, and removing cell debris to obtain filtrate; the filtrate is subjected to DNA dissolution, DNA separation and DNA purification, and a PDRN solution is obtained; wherein the dry weight of the microalgae in the algae liquid is 0.1 to 0.333 g / mL. Through process optimization, the extraction efficiency and purity of the microalgae PDRN are improved, and the extracted PDRN has excellent anti-aging and anti-inflammatory effects.

The invention discloses a peptide with a bone strengthening effect as well as a preparation method and application thereof, and relates to the field of biology. The casein is used as a raw material, the high-activity calcium ion chelating peptide and the directional preparation technology thereof are provided, the technology has the advantages of being high in efficiency, low in cost, easy to operate, good in stability and the like, the high-activity calcium ion chelating peptide can be prepared on a large scale, the utilization value of the casein is improved, and a new way is provided for development of functional natural calcium supplement products.

The invention relates to a preparation method and application of an artificially synthesized exosome with an anti-aging effect, and belongs to the technical field of cosmetics and biology. The artificially synthesized exosome is prepared from hydrogenated lecithin, phytosphingosine, cholesterol, ceramide, squalane, sodium stearoyl glutamate, a surface modification structure, polyol, an active component and water. The preparation method comprises the steps of preparation of an alcohol phase, preparation of a water phase, preparation of a mixed solution and high-pressure homogenization treatment. The artificially synthesized exosome prepared by the preparation method provided by the invention is good in stability and high in anti-aging activity.

The invention discloses a ginseng extract rich in rare saponins and preparation and anti-aging application thereof, and belongs to the technical field of oral beauty and skin care products, the ginseng extract is obtained by fermentation treatment of ginseng powder through phytobacterium plantarum, and the preservation number of the phytobacterium plantarum is GDMCC No.64374. The ginseng extract rich in rare saponin is fermented by specific plant lactobacillus, ginsenoside is efficiently converted into CK and F1, various natural plant components such as ginseng polysaccharide are reserved, and a specific synergistic effect is generated. Through verification, the prepared ginseng extract is high in bioavailability, can more effectively inhibit cell inflammation, and can more effectively recover the mitochondrial function of senescent cells and inhibit cell senescence. The preparation method provided by the invention is high in operability and easy to standardize and intellectualize. Compared with a traditional extraction and purification method, the adopted fermentation method is more environmentally friendly and efficient, and large-scale production can be achieved in industrial production.

The invention discloses a moisturizing and antioxidant composition containing a Campanumoea maculata extract and PDNR, and belongs to the technical field of cosmetics. The composition is prepared from the following components: an extract of barks of the calyx ampelopsis, an extract of fruits of annona squamosa, an extract of leaf cells of desert rose, an extract of fruits of fructus corni and polydeoxyribonucleotide (PDRN), and the composition has good moisturizing and anti-oxidation effects through the synergistic effect of the five components of the extract of the barks of the calyx ampelopsis, the extract of the fruits of the fructus corni and the polydeoxyribonucleotide (PDRN).

The invention relates to the technical field of cosmetics, and provides a composition containing a dendrobium officinale enzymolysis extract, which is prepared from the following components in percentage by mass: 18 to 22 percent of dendrobium officinale enzymolysis extract, 9 to 11 percent of chitosan oligosaccharide, 6.5 to 7.5 percent of bionic moisturizing enhancer, 0.1 to 0.2 percent of sodium alginate, 0.05 to 0.15 percent of citric acid / sodium citrate buffer pair and 60.4 to 65.4 percent of solvent matrix. By combining low-temperature enzymolysis with a biological purification process, efficient extraction of dendrobium candidum polysaccharides and phenolic substances is realized, dendrophenol oxidation inactivation caused by high temperature can be effectively avoided, hydrochloric acid residues in chitosan oligosaccharide preparation are effectively removed, formula irritation is remarkably reduced, and the dendrobium candidum extract is more suitable for sensitive skin care; the components in the composition have a synergistic effect, the dendrobium officinale enzymolysis extract and the enzymolysis chitosan oligosaccharide form a composite moisturizing network, the moisturizing rate is remarkably increased, the oxidation resistance is remarkably improved, and the composition can be applied to products such as essence, masks and freeze-dried powder.

The invention provides a recombinant XVII type collagen and application thereof, and relates to the technical field of protein engineering. The novel recombinant human XVII type collagen is obtained by optimizing and screening collagen structural domain sequences of the human XVII type collagen and then combining the sequences. The recombinant human XVII type collagen is consistent with the corresponding part of the amino acid sequence of the human XVII type collagen, does not generate immunological rejection when being applied to a human body, and can be widely applied to the industries of surgical medical treatment and medical beauty. In addition, the recombinant human XVII type collagen is high in stability, and the problem of protein degradation in the process of expressing recombinant protein by pichia pastoris can be avoided. In addition, the recombinant human XVII type collagen also has the effects of high biological activity, high biological safety, promotion of cell proliferation and differentiation and promotion of hair growth, and can be applied to the field of cosmetics or medicines as a functional component.