19 results about "Pseudoalteromonas" patented technology

The invention discloses a composition with sensitive skin repairing and anti-aging functions as well as an emulsion and application of the composition, and belongs to the technical field of cosmetics. The composition specifically comprises a pseudoalteromonas fermentation product extract, collagen, palmitoyl tripeptide-5, acetylchitosamine, sodium hyaluronate, silanetriol, caffeine and acetyl hexapeptide-8, and can promote the generation of laminin-5 and integrin beta1, promote the vitality of keratinocytes and fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, and promote the growth of the keratinocytes and the fibroblasts. The generation of inflammatory factors (IL-6) is inhibited; the generation of collagen IV and collagen VII is promoted; the invention further provides an emulsion prepared from the composition, and the optimal formula of the emulsion is obtained through optimization. The active composition disclosed by the invention has a wide application prospect in the field of cosmetics, and particularly has important significance in sensitive skin repair and anti-aging directions.

The invention discloses bacillus velezensis Bve1910, which is preserved in the China Center for Type Culture Collection (CCTCC) and has the preservation number of CCTCC NO. M 20221965. The strain and metabolites thereof have strong antagonistic effects on more than ten types of marine culture animal pathogenic bacteria such as vibrio harveyi, vibrio erwiniae, vibrio canbayi, vibrio parahaemolyticus, vibrio alginolyticus, vibrio anguillarum, vibrio cholerae, vibrio shewanii, vibrio rotifer, pseudoalteromonas and photobacterium damarae, are strong in antibacterial activity and wide in antibacterial spectrum, and can be used for preparing marine culture animal pathogenic bacteria. According to the preparation method, the traditional Chinese medicine composition is simple in preparation method and rich and stable in metabolite, an ammonium sulfate precipitation extract, a hydrochloric acid precipitation methanol extraction extract, an ethyl acetate extraction extract and a chloroform extraction extract all have a broad-spectrum bacteriostatic effect, functional substances playing the broad-spectrum antagonistic bacteriostatic effect are compounded and diversified, and the directional selection effect on bacteria in the environment and ecology is not generated; and pathogenic bacteria are not easy to generate tolerance, so that the method has good application safety and ecological safety properties.

The present application belongs to the technical field of daily cosmetics, and particularly relates to a vitamin E moisturizing skin cream added with peony fresh flower stock solution and a preparation method thereof. The moisturizing skin cream provided by the present application comprises, in weight parts, 1-10 parts of peony fresh flower stock solution fermentation liquor, and 0.1-2 parts of vitamin E, wherein the peony fresh flower stock solution fermentation liquor is obtained by two-step fermentation of peony fresh flower stock solution with a compound microbial agent of two-split yeast, Pseudoalteromonas and galactose yeast-like bacteria in sequence. The product obtained by compounding the vitamin E cream with the fermented peony stock solution fermentation liquor has good moisturizing property, improves the side effect of skin dryness caused by long-term use of vitamin E cream, and has good anti-allergy effect, and the prepared moisturizing cream has an inhibition rate of hyaluronidase of more than 80%.

The application belongs to the technical field of skin care product preparation, and discloses a natural polysaccharide composition, a preparation method and application thereof. The natural polysaccharide composition is composed of the following components in percentage by mass: Pseudoalteromonas ferment product extract 50-70%, locust bean gum 10-15%, cassia gum 5-10%, and psyllium seed gum 15-25%. The Pseudoalteromonas ferment product extract refers to extracellular polysaccharide produced by fermentation of Pseudoalteromonas mucosa DSM15203; the locust bean gum is obtained by extraction and mannanase enzymolysis of seeds of locust trees; the cassia gum is obtained by extraction and mannanase enzymolysis of seed endosperm of cassia plants; and the psyllium seed gum is obtained by extraction and xylanase enzymolysis of seed husks of psyllium.

The invention provides a pseudoalteromonas kappa-carrageenase mutant. The amino acid sequence of the mutant is as shown in SEQ ID NO: 1; compared with wild-type kappa-carrageenase (WT), the enzyme activity of the mutant is improved by 14.2%; after the G198S and the WT are treated at 60 DEG C for 30 minutes, the residual activities of the G198S and the WT are 35.1% and 7.8% respectively; at 50 DEG C, compared with WT, the half-life period of the G198S is prolonged by 27.8 min; molecular docking analysis shows that the increase of non-covalent interaction around a mutation point may be a reason for the improvement of the thermal stability of the mutant; molecular dynamics simulation analysis shows that the improvement of the thermal stability and the catalytic activity of G198S can be attributed to the flexibility increase of F6 and small structural deviation in part of rings and beta-tablets; the discovery provides a useful strategy for improving the thermal stability and enzymatic activity of the kappa-carrageenase and better industrial application of the kappa-carrageenase.

The invention relates to the technical field of raw materials of skin care products, and particularly discloses a composition YvesXSea with a slow-release repairing effect and application of the composition YvesXSea. The invention relates to a composition YvesXSea with slow-release and repair effects. The composition YvesXSea is prepared by compounding chondrus crispus, a soybean root nodule extract, hydrolyzed algal gum, a pseudoalteromonas fermentation product extract and a radix arnebiae extract. The preparation method has the advantages that the thickening effect is achieved, and meanwhile the good slow-release effect is achieved.

The invention provides a biological enhancement-catalytic oxidation treatment system for high-salinity wastewater and an operation process thereof, and relates to the technical field of high-salinity wastewater treatment.The biological enhancement-catalytic oxidation treatment system comprises a biological enhancement unit, a catalytic oxidation unit and a salt separation crystallization unit which are sequentially communicated; a composite salt-tolerant flora in the biological enhancement unit is fixed on a sodium alginate-activated carbon-SiO2 composite carrier; the composite salt-tolerant bacterial flora comprises a salt-tolerant bacterial flora which is formed by compounding Halomonas, Thauera and Pseudoalteromonas, and the salt-tolerant bacterial flora is formed by compounding Halomonas, Thauera and Pseudoalteromonas; the catalytic oxidation unit is provided with a Fe3O4 (at) TiO2 / graphene quantum dot composite catalyst and an LED light source; and the salt separation crystallization unit is used for crystallizing and producing NaCl and Na2SO4. According to the invention, the technical problems of low treatment efficiency and flora inactivation of traditional biological treatment in a high-salinity environment are solved, the technical effects of improving stability and treatment efficiency are achieved, and the purpose of near-zero discharge of high-salinity wastewater is achieved.

The present invention relates to the field of biotechnology, and in particular to a constitutive promoter P256 and its activity identification method and application. The constitutive promoter P256 is derived from the Arctic psychrophilic bacterium Pseudoalteromonas fuliginea BSW20308. After amplification, it is cloned into the upstream of the green fluorescent protein coding region of the pRU1701 plasmid after enzyme digestion using a seamless assembly kit to obtain a recombinant plasmid. The recombinant plasmid and the blank vector pRU1701 plasmid are then transformed into Escherichia coli DE3, cultured in LB medium, and samples are taken after inoculation to detect their fluorescence value and OD. 600 The activity of the constitutive promoter P256 was identified using unit fluorescence intensity. The constitutive promoter P256 of the present invention has the potential to be applied to other different types of prokaryotic bacteria, including special extreme environment microorganisms, and can be used to increase the expression of exogenous proteins.

This invention discloses a Bacillus tergenta Bte2005, with accession number M 2026736. This invention relates to Bacillus terrestris Bte2005, isolated from the hatching system of healthy Litopenaeus vannamei eggs. This strain and its metabolites exhibit strong antagonistic effects against more than ten pathogenic bacteria of marine aquaculture animals, including Vibrio harveyi, Vibrio Erwini, Vibrio campbellii, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio anguillarum, Vibrio cholerae, Vibrio shewani, Vibrio rotiferus, Pseudomonas pseudoalteromonas, and luminescent bacteria of mermaids. It demonstrates strong antibacterial activity and a broad antibacterial spectrum. Bacillus terrestris Bte2005 metabolites are abundant and stable. Its ammonium sulfate precipitate extract, hydrochloric acid precipitate methanol extract, ethyl acetate extract, and chloroform extract all exhibit broad-spectrum antibacterial activity. The functional substances that exert its broad-spectrum antagonistic antibacterial effect are complex and diverse, and it does not exert a targeted selection effect on bacteria in the environment, nor does it easily induce tolerance in pathogens. Therefore, it possesses good application safety and ecological safety properties.

Skin care compositions and, preferably, skin care compositions providing one or more improvements in skin appearance and, in some cases, also in skin health are disclosed herein. In accordance with an aspect of the invention, provided is a skin care composition comprising hyaluronic acid or a salt thereof; vitamin E; a peptide; an extract comprising one or more of silybum marianum extract or pseudoalteromonas ferment extract; and water in an amount of about 45 wt.% or more, based on the total weight of the skin care composition.

This invention relates to a disaccharide exoglycosidase β-agarase with both galactosidase and β-galactosidase activities and its applications, belonging to the field of biotechnology. This invention discloses for the first time a method for developing a β-agarase from *Pseudomonas alterniflora* (…). Pseudoalteromonas An enzyme, ORF0276, and its encoding gene, possessing both galactosidase and disaccharide exonuclease activities, were obtained from the genome of strain Q02 (sp.). This enzyme exhibits strong thermostability, high pH tolerance, and stable physicochemical properties, thus possessing potential for industrial applications. When degrading agar polysaccharide substrates, it can act on the β-1,4 glycosidic bonds of the sugar chains to produce a product primarily composed of neo-agarbiose, which can be used for the industrial preparation of neo-agarbiose (NA2). This research provides a molecular template and theoretical reference for the discovery of such multi-functional tool enzymes.

This invention relates to an endonuclease β-agarase, its mutants, mutation methods, and applications, belonging to the field of biotechnology. This invention discloses for the first time a mutant of *Pseudomonas alterniflora* (…). Pseudoalteromonas An endonuclease β-agarase, ORF2093, was obtained from strain A601. This endonuclease β-agarase ORF2093 exhibits certain thermal stability, wide pH tolerance, and stable physicochemical properties, thus possessing potential for industrial application. This invention employs rational design and site-directed mutagenesis technology to mutate the serine residue at position 373 in the non-catalytic region of the endonuclease β-agarase ORF2093 to asparagine. The resulting mutant showed significantly enhanced relative activity compared to the wild type, and it was found that the mutant enzyme possesses the ability to degrade novel agaroctaose substrates.

A topical cosmetic skincare composition comprising at least one prebiotic, at least one postbiotic comprising Lactobacillus, and a dermatologically acceptable solvent. A method of preparing the composition comprising combining at least one prebiotic, at least one postbiotic comprising Lactobacillus, and a dermatologically acceptable solvent, a method of improving the appearance of the skin comprising applying the composition in an effective amount, and use of the composition as a cosmetic treatment to improve skin appearance are also included. The composition may include a second postbiotic, which may be Saccharomyces, Acetobacter xylinium, black tea ferment, a different strain of Lactobacillus to the first postbiotic, or Pseudoalteromonas ferment extract. The postbiotics may have a prebiotic effect. The prebiotics may be squalene, olive oil, rice bran oil, Undaria pinnatifida (brown macroalgae) extract, Sargassum horneri, niacinamide (vitamin B3) or tocopherol (vitamin E). The Lactobacillus may be L. rhamnosus. The prebiotics may comprise 0.5-5% of the composition and the postbiotics may comprise 0.5-6% of the composition. The Lactobacillus may form 0.005-2% of the composition. The solvent may form 50-90% of the composition and may be water, pentylene glycol or propanediol. The composition may further comprise emulsifiers, preservatives, moisture increasing components, film-forming agents and pH adjusters.

The invention relates to the field of cosmetics, in particular to deep sea fish skin collagen peptide, a preparation method thereof and cosmetics. Drying the fat-removed deep sea fish skin to obtain dried deep sea fish skin; mixing the first fermentation seed solution and the second fermentation seed solution to obtain a mixed fermentation seed solution; inoculating the mixed fermentation seed solution into a fermentation culture medium for fermentation; the first fermentation seed solution is a pseudoalteromonas gelatinolyticum seed solution; the second fermentation seed solution is a lactobacillus plantarum seed solution; based on the total volume of the fermentation culture medium, the fermentation culture medium is prepared from 20.0-100.0 g / L of dried deep sea fish skin and 0.5-1.5 g / L of sodium carbonate; 0.5 to 1.5 g / L of disodium hydrogen phosphate; 0.1 to 0.5 g / L of monopotassium phosphate; a solvent of the fermentation culture medium is artificial seawater. The deep sea fish skin collagen peptide has a stable collagen structure, is rich in unique collagen functional peptide fragments, and can be applied to the field of cosmetics.

The invention relates to the field of biotechnology and microbiology, in particular to Pseudoalteromonas wwarhai M044 for producing alteromide A and application of the Pseudoalteromonas wwarhai M044, and the provided strain can be used for stably synthesizing secondary metabolites with biological activity, such as alteromide A and the like. Functional verification shows that the strain and a purified product thereof have remarkable inhibitory activity on staphylococcus aureus and bacillus subtilis, and the minimum inhibitory concentration of the strain and the purified product thereof is measured; meanwhile, a biosynthetic gene cluster of the strain is successfully positioned through genome analysis, and it is indicated that the strain has a definite synthetic route and stable product synthesis capacity. Compared with a traditional antibacterial compound, the strain and the product of the strain are natural in source, safe, environment-friendly and clear in biosynthesis route, an efficient and controllable production process can be realized, and a new microbial resource and a technical route are provided for developing a novel marine-derived antibacterial agent.

The present application relates to the field of cosmetic technology, especially to the field of IPC A61K8, further, it relates to a skin care composition for balancing skin water and oil and anti-inflammatory and application thereof. The raw materials at least include at least two of Nymphaeaceae extract, Bombaxaceae extract and Pseudoalteromonas ferment extract. The composition prepared by the present application not only can improve the oil control effect of the composition, but also has good moisturizing and anti-inflammatory performance, so that the skin achieves water and oil balance; in addition, it also has good stability.

SKIN CARE COMPOSITIONS Skin care compositions and, preferably, skin care compositions providing one or more improvements in the appearance of the skin and, in some cases, in the health of the skin are disclosed herein. According to one aspect of the invention, there is provided a skin care composition comprising hyaluronic acid or a salt thereof, vitamin E, a peptide, an extract comprising one or more Silybum marianum extracts or a Pseudoalteromonas ferment extract, and water in an amount of about 45% by weight or more, based on the total weight of the skin care composition.

The invention belongs to the technical field of skin care product preparation, and discloses a natural-source polysaccharide composition as well as a preparation method and application thereof. The natural source polysaccharide composition is prepared from the following components in percentage by mass: 50 to 70 percent of pseudoalteromonas fermentation product extract, 10 to 15 percent of locust bean gum, 5 to 10 percent of cassia gum and 15 to 25 percent of plantain seed gum. The Pseudoalteromonas sp. Fermentation product extract is an extracellular polysaccharide generated by fermentation of Pseudoalteromonas sp. DSM15203; the locust bean gum is obtained by performing extraction and mannase enzymolysis on locust tree seeds; the semen cassiae gelatin is obtained by extracting seed endosperm of a semen cassiae plant and performing enzymolysis with mannase; the plantain seed gum is obtained by extracting seed husks of plantain herbs and performing enzymolysis with xylanase.

In alternative embodiments, provided are compositions, including products of manufacture and kits, and methods, for producing enhanced marine bacterial strains with the ability to produce stimulatory products that amplify their probiotic effects for reef restoration and biotechnology applications. In alternative embodiments, genetically engineered marine bacteria (such as Nereida, Vibrio, Pseudoalteromonas and / or Roseobacter bacterium) as provided herein, and compositions, products of manufacture, kits and methods as provided herein, are used in biomaterial production, probiotic use in aquaculture and / or for environmental restoration purposes.