6 results about "Pseudoalteromonas" patented technology

The present application belongs to the technical field of daily cosmetics, and particularly relates to a vitamin E moisturizing skin cream added with peony fresh flower stock solution and a preparation method thereof. The moisturizing skin cream provided by the present application comprises, in weight parts, 1-10 parts of peony fresh flower stock solution fermentation liquor, and 0.1-2 parts of vitamin E, wherein the peony fresh flower stock solution fermentation liquor is obtained by two-step fermentation of peony fresh flower stock solution with a compound microbial agent of two-split yeast, Pseudoalteromonas and galactose yeast-like bacteria in sequence. The product obtained by compounding the vitamin E cream with the fermented peony stock solution fermentation liquor has good moisturizing property, improves the side effect of skin dryness caused by long-term use of vitamin E cream, and has good anti-allergy effect, and the prepared moisturizing cream has an inhibition rate of hyaluronidase of more than 80%.

The invention provides a biological enhancement-catalytic oxidation treatment system for high-salinity wastewater and an operation process thereof, and relates to the technical field of high-salinity wastewater treatment.The biological enhancement-catalytic oxidation treatment system comprises a biological enhancement unit, a catalytic oxidation unit and a salt separation crystallization unit which are sequentially communicated; a composite salt-tolerant flora in the biological enhancement unit is fixed on a sodium alginate-activated carbon-SiO2 composite carrier; the composite salt-tolerant bacterial flora comprises a salt-tolerant bacterial flora which is formed by compounding Halomonas, Thauera and Pseudoalteromonas, and the salt-tolerant bacterial flora is formed by compounding Halomonas, Thauera and Pseudoalteromonas; the catalytic oxidation unit is provided with a Fe3O4 (at) TiO2 / graphene quantum dot composite catalyst and an LED light source; and the salt separation crystallization unit is used for crystallizing and producing NaCl and Na2SO4. According to the invention, the technical problems of low treatment efficiency and flora inactivation of traditional biological treatment in a high-salinity environment are solved, the technical effects of improving stability and treatment efficiency are achieved, and the purpose of near-zero discharge of high-salinity wastewater is achieved.

This invention discloses a Bacillus tergenta Bte2005, with accession number M 2026736. This invention relates to Bacillus terrestris Bte2005, isolated from the hatching system of healthy Litopenaeus vannamei eggs. This strain and its metabolites exhibit strong antagonistic effects against more than ten pathogenic bacteria of marine aquaculture animals, including Vibrio harveyi, Vibrio Erwini, Vibrio campbellii, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio anguillarum, Vibrio cholerae, Vibrio shewani, Vibrio rotiferus, Pseudomonas pseudoalteromonas, and luminescent bacteria of mermaids. It demonstrates strong antibacterial activity and a broad antibacterial spectrum. Bacillus terrestris Bte2005 metabolites are abundant and stable. Its ammonium sulfate precipitate extract, hydrochloric acid precipitate methanol extract, ethyl acetate extract, and chloroform extract all exhibit broad-spectrum antibacterial activity. The functional substances that exert its broad-spectrum antagonistic antibacterial effect are complex and diverse, and it does not exert a targeted selection effect on bacteria in the environment, nor does it easily induce tolerance in pathogens. Therefore, it possesses good application safety and ecological safety properties.

This invention relates to a disaccharide exoglycosidase β-agarase with both galactosidase and β-galactosidase activities and its applications, belonging to the field of biotechnology. This invention discloses for the first time a method for developing a β-agarase from *Pseudomonas alterniflora* (…). Pseudoalteromonas An enzyme, ORF0276, and its encoding gene, possessing both galactosidase and disaccharide exonuclease activities, were obtained from the genome of strain Q02 (sp.). This enzyme exhibits strong thermostability, high pH tolerance, and stable physicochemical properties, thus possessing potential for industrial applications. When degrading agar polysaccharide substrates, it can act on the β-1,4 glycosidic bonds of the sugar chains to produce a product primarily composed of neo-agarbiose, which can be used for the industrial preparation of neo-agarbiose (NA2). This research provides a molecular template and theoretical reference for the discovery of such multi-functional tool enzymes.

This invention relates to an endonuclease β-agarase, its mutants, mutation methods, and applications, belonging to the field of biotechnology. This invention discloses for the first time a mutant of *Pseudomonas alterniflora* (…). Pseudoalteromonas An endonuclease β-agarase, ORF2093, was obtained from strain A601. This endonuclease β-agarase ORF2093 exhibits certain thermal stability, wide pH tolerance, and stable physicochemical properties, thus possessing potential for industrial application. This invention employs rational design and site-directed mutagenesis technology to mutate the serine residue at position 373 in the non-catalytic region of the endonuclease β-agarase ORF2093 to asparagine. The resulting mutant showed significantly enhanced relative activity compared to the wild type, and it was found that the mutant enzyme possesses the ability to degrade novel agaroctaose substrates.

The invention relates to the field of biotechnology and microbiology, in particular to Pseudoalteromonas wwarhai M044 for producing alteromide A and application of the Pseudoalteromonas wwarhai M044, and the provided strain can be used for stably synthesizing secondary metabolites with biological activity, such as alteromide A and the like. Functional verification shows that the strain and a purified product thereof have remarkable inhibitory activity on staphylococcus aureus and bacillus subtilis, and the minimum inhibitory concentration of the strain and the purified product thereof is measured; meanwhile, a biosynthetic gene cluster of the strain is successfully positioned through genome analysis, and it is indicated that the strain has a definite synthetic route and stable product synthesis capacity. Compared with a traditional antibacterial compound, the strain and the product of the strain are natural in source, safe, environment-friendly and clear in biosynthesis route, an efficient and controllable production process can be realized, and a new microbial resource and a technical route are provided for developing a novel marine-derived antibacterial agent.