Pseudoalteromonas sp.BJ01 with antifungal activity

A technology of Pseudomonas alternata and fermentation bacteria, which is applied in the field of microbial screening and can solve problems such as loss

Active Publication Date: 2017-12-26
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially in the case of low temperature, high humidity and poor ventilation conditions in the winter warm shed, once the disease occurs, if it cannot be prevented and controlled in time, it will cause serious losses.

Method used

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  • Pseudoalteromonas sp.BJ01 with antifungal activity
  • Pseudoalteromonas sp.BJ01 with antifungal activity
  • Pseudoalteromonas sp.BJ01 with antifungal activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Screening of bacterial strains

[0021] Pseudoalteromonas sp.BJ01 was derived from sea mud samples collected during China's 6th Arctic Scientific Expedition. Zobel 2216E medium was used for streak screening, purification, and 16S rDNA species identification. The purified strains were respectively inserted into Erlenmeyer flasks equipped with 2216E liquid medium, and cultured with shaking at 25°C and 150rpm for 24h to be used as bacterial suspension.

[0022] For the screening of antibacterial strains, common plant pathogenic bacteria such as Fusarium oxysporum were selected as indicator bacteria, plant pathogenic fungi were inoculated on PDA solid medium, and after culturing at 25°C for 48 hours, a piece of mycelium was taken and added to the sterile Shake fully in the test tube of water, after filtering mycelia with two layers of sterilized gauze, dilute to obtain phytopathogenic fungal spore suspension (10 5 cell / mL). The antibacterial activity of the str...

Embodiment 4

[0027] Example 4. Biochemical characteristics of Pseudoalteromonas sp.BJ01 strain

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Abstract

The invention provides a pseudoalteromonas sp.BJ01 with antifungal activity, which has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 14505, and application of the screened pseudoalteromonas in preparation of plant pathogenic inhibitor products. According to the pseudoalteromonas sp.BJ01 provided by the invention, a bacteriostasis experiment shows that the bactericidal agent has inhibiting effect on various plant pathogenic, and the prevention effect on greenhouse cucumber gray mold under the low temperature condition of 10 to 15 DEG C can reach 66.7%. The bacterial strain, provided by the invention, has potential application prospect in the pollution-free biological prevention and control of the plant diseases.

Description

technical field [0001] The invention belongs to the technical field of microbial screening, and in particular relates to a strain of arctic pseudomonas with antifungal activity. Background technique [0002] Crop diseases caused by fungi have become an important issue threatening global food security. The increase in the variety and quantity of crops, the global trade and transportation of crops, the changes in global climate and environment, and the adaptation of pathogenic fungi to new ecosystems have all led to the increasing severity of fungal diseases. At present, artificially synthesized fungicides are still the main and most effective means to prevent and control fungal diseases of crops, but they also cause harm to humans and the environment. Microbial control of crop fungal diseases has the advantages of safety, high efficiency, and environmental friendliness, and has become an alternative to chemical fungicides that has attracted widespread attention at home and a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 李江何培青
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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