The invention discloses a breeding method for aseptic seeding and test tube seedling formation of orchids and the adopted broad-spectrum culture medium. In this method, a healthy orchid mother plant is selected, and artificially pollinated when it blooms, and the fruit that develops to maturity after pollination and has not cracked is used as an explant, or the orchid fruit that is basically mature and not cracked is taken from the wild as an explant. Implants undergo reproductive steps such as aseptic sowing, seed germination, strong seedling cultivation, and test-tube seedling transplantation. The specially prepared medium is used for aseptic sowing and seed germination to obtain small plants, and then the small plants are cultivated on the specially prepared strong seedling medium, and the seedlings are hardened under natural light in the greenhouse for 7-14 hours before being released from the bottle. One day, the test-tube seedlings were taken out, the root medium was washed off, and they were planted in a mixed substrate of bark, bluestone and peat, and further cultivated into seedlings. The invention has the characteristics of high seed germination rate, fast seedling growth, good seedling quality, low cost, etc., can obtain a large number of test-tube seedlings in a short period of time, and the transplanting survival rate of the seedlings can be kept above 90%. An effective way can be provided for the production of orchid seedlings.