Bacillus amyloliquefaciens growing in disease-preventing and growth-promoting plant and application thereof

A technology of Bacillus amyloliquefaciens and spore amyloliquefaciens, which is applied in the field of Bacillus amyloliquefaciens, can solve the problems such as bacteria prevention being easily affected by external environmental conditions, unsatisfactory disease prevention effect, affecting disease prevention effect, etc. The effect of reducing pesticide residues and being easy to implement

Active Publication Date: 2011-01-19
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the strains producing biopesticides are isolated and screened from soil microorganisms in the rhizosphere of plants. Since these soil biocontrol bacteria are easily affected by external environmental conditions, in the competition with microorganisms inhabiting the soil, plant rhizosphere or leaf periphery It is not easy to colonize and dominate for a long time, and the disease prevention effect is not ideal, which greatly affects their actual disease prevention effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0013] Specific embodiment one: In this embodiment, Bacillus amyloliquefaciens is endogenous to disease prevention and growth-promoting plants, which is Bacillus amyloliquefaciens TF28, which is Bacillus amyloliquefaciens and belongs to the genus Bacillus. It has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation number is CGMCC No.4038, and the preservation date is July 26, 2010; the endogenous Bacillus amyloliquefaciens TF28 of the disease prevention and growth promotion plant is good for Gram-positive Aerobic bacteria, rod-shaped, 1.5μm-2.5μm long, 0.5μm-1.5μm wide, endophytic spores, no flagella; milky white colonies formed on beef extract peptone medium, wrinkled, irregular edges, colony center The protrusions are ridged.

[0014] In this embodiment, the endogenous Bacillus amyloliquefaciens in plants for disease prevention and growth promotion is initially identified as Bacillus according ...

specific Embodiment approach 2

[0027] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the endogenous Bacillus amyloliquefaciens in disease prevention and growth-promoting plants has a positive glucose fermentation test, a positive starch hydrolysis test, a positive gelatin liquefaction test, a positive V.P reaction, and a contact enzyme reaction. Positive, positive methyl red reaction, positive nitrate reduction test, positive citrate utilization, good growth, growth pH value of 6.5-8.5, growth temperature of 30-37°C. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0028]Specific embodiment three: the application of Bacillus amyloliquefaciens in plants for disease prevention and growth promotion in this embodiment: 1. Take the bacterial liquid of bacterial strain TF28 frozen at -70°C, inoculate it on LB solid medium by streaking, and keep the temperature at 30°C Cultivate in the box for 18-20 hours, pick a single colony and inoculate it in 5ml of LB liquid medium, and culture it with shaking at 30°C for 18-20 hours to obtain the activated bacterial liquid; 2. Inoculate the activated bacterial liquid in the In a 500ml Erlenmeyer flask with 100ml NYD culture medium, culture at 30°C for 32-50 hours, with a rotation speed of 150-250r / min, and the concentration of the bacterial liquid reaches 2-4 billion / ml at the end of the fermentation, which is the fermentation liquid of the strain; 3. To be controlled The seeds of the method are pre-soaked in water for 5-7 days, and then soaked in the 50-fold dilution of the fermentation broth of the strai...

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Abstract

The invention relates to a bacillus amyloliquefaciens growing in a disease-preventing and growth-promoting plant and application thereof, relating to bacillus amyloliquefaciens and the application thereof. The bacillus amyloliquefaciens TF28 growing in the disease-preventing and growth-promoting plant belongs to bacillus, has the storage number of CGMCC No.4038 and the storage data of July 26, 2010. The application of the bacillus amyloliquefaciens TF28 comprises the following steps of: (1) preparing activated bacilli liquid; (2) inoculating the activated bacilli liquid in an NYD culture fluid to obtain a strain fermentation solution; and (3) soaking seeds in a dilution solution 50 times of the strain fermentation solution. The strain TF28 in the invention can be colonized, reproduced and transmitted in plants, can simultaneously generate two antibacterial substances, i.e. antimicrobial proteins and lipopeptide antibiotic and has the multiple efficacy of preventing diseases, stimulating growth, increasing the yield, improving the quality, widening an antibacterial spectrum, and the like. Rice seeds treated by the dilution solution 50 times of the fermentation solution of the strain TF28 reach a room-temperature bakana resistance rate over 84.6 percent.

Description

technical field [0001] The present invention relates to bacillus amyloliquefaciens and application thereof. Background technique [0002] Plant diseases are one of the main factors affecting high, stable and high-quality agricultural production. The annual losses caused by diseases in agriculture account for about 20% of the total output value, and the annual direct economic losses caused by diseases are as high as more than 5 billion. Chemical pesticides are used for prevention and control, but chemical pesticides have always had many insurmountable disadvantages such as drug resistance, environmental pollution, pesticide residues, biodiversity reduction, and human and animal poisoning. [0003] Biopesticides meet the requirements of modern society for agricultural production and pesticides due to their low toxicity to humans and animals, good environmental compatibility, and resistance to diseases and insect pests, and have broad development space. Most of the strains pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCY02A40/10
Inventor 张淑梅王玉霞李晶赵晓宇孟利强张先成张云湖
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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