The invention discloses a preparation method of a microbial inoculum of
mycorrhizal fungi wax mushroom bicolor. The preparation method of the microbial inoculum mainly comprises preparation of a culture medium and preparation of the microbial inoculum. The formula of the
liquid culture medium is as follows: 10g of glucose, 2g of
maltose, 1g of
yeast extract (
yeast extract), 0.5 g of
monopotassium phosphate, 0.25 g of diammonium
hydrogen phosphate, 0.15 g of
magnesium sulfate, 5mL of
calcium chloride dihydrate with the concentration of 1%, 2.5 mL of
sodium chloride with the concentration of 1% and 1.2 mL of
ferric chloride with the concentration of 1% are dissolved in each
liter of
distilled water. The formula for preparing the
solid culture medium is consistent with that of the
liquid culture medium, and 15 g of
agar needs to be added per
liter. The culture medium needs high-temperature and high-pressure sterilization. The method comprises the following steps:
cutting a
solid microbial inoculum into small blocks of about 1cm < 3 >, putting one small block in the center of a culture dish, pasting one side with hyphae on a culture medium, sealing with a sealing film, and culturing in a dark place to obtain the
solid microbial inoculum. In the culture of the liquid
fungicide, small solid
fungicide blocks with hyphae need to be put into a sterilized
liquid culture medium, 15-20 small blocks are put into each conical flask (250mL conical flask, and 200mL liquid culture medium is put into each conical flask), and the conical flasks in which the
fungicide blocks are put are put into a shaking table to be cultured in a dark place at the temperature of 25 DEG C and the rotating speed of 160 revolutions. The method provided by the invention can be used for rapidly and massively preparing the bicolor
wax mushroom microbial inoculum, and is convenient for experiment and production practice of the bicolor
wax mushroom microbial inoculum. The culture medium is low in cost, and the microbial inoculum is stable in quality.