358 results about "Erlenmeyer flask" patented technology

The invention relates to a pretreatment method for measuring 14C abundance of an organic compound type sample. The method includes transferring carbon atoms in the organic compound into CO2 molecules through combustion, reacting CO2 with a NaOH solution to obtain a saturate NaHCO3 solution, heating the saturate NaHCO3 solution to obtain a saturate Na2CO3 solution, and adopting the saturate Na2CO3 solution for 14C abundance tests. The invention also provides a device used for the method. The device mainly comprises an oxygen cylinder, a nitrogen cylinder, a cold trap A, a three-way stopcock A, a combustion chamber, a cold trap B, a stone, a cold trap C, a three-way stopcock B, a conical flask, a cold trap D, a three-way stopcock C, a U-shaped gas sample collector, a three-way stopcock D, a vacuum gauge, a vacuometer, a piston D and a vacuum pump. On the basis of guaranteeing the measuring accuracy, the method largely simplifies the chemical treatment process, increases the time efficiency and reduced the cost.

The invention discloses a citric acid loofah sponge preparation method and an application technology. The preparation method provided by the invention is characterized by comprising the following steps of: adding 18-25 wt% of citric acid, 18-25 wt% of saponified loofah sponge and 50-60 wt% of water into a plugged Erlenmeyer flask, putting on a plug, carrying out refluxing at the temperature of 60-70 DEG C for 2-3h with stirring, heating up to the temperature of 110-120 DEG C, reacting for 2-3h, cooling, washing by using deionized water, carrying out pumping filtration until a filtrate is neutral, washing by using a few ethanol, and drying in a baking oven of 75 DEG C to obtain the citric acid loofah sponge. The citric acid loofah sponge has strong adsorption capability, can directly used for the adsorption and elution of various metal ions and organic dyes in a water body, is characterized by high adsorption efficiency, fast adsorption speed, good physical and chemical stability and excellent mechanical stability, and can be used within a wide soda acid range. Simultaneously, the citric acid loofah sponge has regeneration capability and is a natural green adsorbent.

A liquid fermentation process for the Chinese pilous spore includes such steps as getting bacterial spawn, Erlenmeyer flask culture of bacterial spawns, liquid seed culture, and liquid fermenting. Its advantags are high successful rate, low cost and no environmental pollution. The resultant mycelia can be used for medical or health-care product. The fermented liquid can be used as oral liquir or beverage.

The invention discloses a preparation method for a mushroom residue charcoal adsorbent and an application thereof. The method comprises the following steps: 1) drying mushroom residue, crushing and screening, thereby acquiring mushroom residue powder; 2) charring the mushroom residue powder into a charring furnace, and then cooling to room temperature, taking out and screening, thereby acquiring mushroom residue charcoal powder; 3) adding the mushroom residue charcoal powder into a conical flask containing deionized water, oscillating in a shaking table, removing an aqueous solution after cleaning, adding deionized water for repeatedly cleaning and drying, thereby acquiring the mushroom residue charcoal adsorbent. The mushroom residue charcoal adsorbent prepared according to the invention has average specific surface area reaching up to 200m<2> / g and average pore volume reaching about 0.15cm<3> / g, has a large amount of phi conjugated aromatic structures, has a universal adsorption capacity to heavy metal positive ions and can effectively remove lead, cadmium, zinc and copper from water at the maximal removal rate of 98.4%, 91.1%, 91.8% and 97.9%, respectively.

The invention discloses a method for producing a rhodobacter sphaeroides microbial fertilizer from citric acid waste water, and belongs to the microorganism field. The method comprises the following steps: a. diluting citric acid waste water with clear water till the COD concentration is 12000-15000 mg / L, adjusting the pH to 6.5-7.5 with a sodium hydroxide aqueous solution, sterilizing the solution at 115-126 DEG C for 10-35 min; b. inoculating rhodobacter sphaeroides preserved by a slant into an erlenmeyer flask containing the citric acid waste water, performing shaking culture at 30-37 DEG C under 120-200 rpm for 24-48 hours to obtain liquid seeds; c. inoculating the liquid seeds into a fermentor containing the citric acid waste water with a volume ratio of 2%-20%, culturing the seeds in sterile air at 25-37 DEG C for 12-48 hours to obtain fermentation broth; d. determining the cultured fermentation broth by a spectrophotometer, packaging the broth when the OD660 value is 1.0-1.5 orthe bacterial count reaches 109-1010 CFU / ml so as to obtain a finished product. Compared with the prior art, the invention produces a microbial fertilizer from citric acid waste water, and has the characteristics of low production cost, energy saving and environmental protection, good product application effect, etc.

A device for testing the rate of inflammable gas given off when matter meets water is characterized in that a constant voltage separating funnel with a side pipe is communicated with a conical beaker by a three way pipe; the conical beaker is arranged in a thermostatic water bath circulator which is internally provided with water bath for heating and cooling; the side pipe of the constant voltage separating funnel and the outlet of the bottom thereof are respectively provided with a switch; the outlet at the side of the three way pipe is communicated with the air inlet of a flow meter; the air outlet of the flow meter is communicated with a surge flask; the input end of a monitor is connected with the output end of the flow meter. The device can be used for judging fatalness when the matter meets wet, has the real time supervisor control function, does not need manual time-keeping, weighting and calculation; after the testing is over, the starting time of the testing is input, and then the real time monitoring data can be led out, thus avoiding the problem that the manual weighting can not exactly measure the rate of inflammable gas given off when the matter meets water, meanwhile, saving the precious manpower and time and greatly improving work efficiency.

The invention discloses an experimental method for raising gas desorption property, characterized by based on the experimental study of gas absorption, desorption and permeability by existing non-mechanical method, using an experimental device which comprises a gas bottle, a pressure-tolerant gas pipe, a valve, a gas storage tank, a drying agent, a pressure sensor, a desorption pot, a positive electrode, a constant temperature water tank, a guide wire, a high-voltage variable-frequency power source, a vacuum pump, a data collection card, a computer, a rubber pipe, a measuring tube, and an Erlenmeyer flask, putting an experimental coal sample in the desorption pot, using the high-voltage variable-frequency electric field to change the physicochemical properties of the coal and allow the moisture in the coal and different charged ions dissolved in the water molecules to directionally move, thus free and adsorbed gas is increased, and the desorption property is raised. According to the invention, compared with the gas desorption property obtained by using a natural coal sample, when the high-voltage variable-frequency power source output voltage is 2kV and the frequency is 40Hz, the desorption is averagely raised by 11.42%.

The invention discloses a formula for the culture medium of cordyceps militaris liquid strains and a method for culturing the same. The invention is characterized in that the method comprises the following steps: preparing a culture solution with the pH value being 7.5 from the following components by percentage: 0.8% of peptone, 2% of glucose, 0.1% of ammonium citrate, 0.1% of magnesium sulfate, 0.1% of monopotassium phosphate, 0.002% of vitamin B1 and 92.9% of water; filling containers, such as erlenmeyer flasks, saline bottles or preserving jars, with the culture solution; carrying out regular autoclaving on the culture solution; then, grafting the slant mother culture of cordyceps militaris onto the culture solution; and carrying out standing and culturing for 7 to 10 days in a light-blocking and airtight culture room with the temperature being 18 to 22 DEG C to obtain the cordyceps militaris liquid strains. The invention dispenses with fermentation tanks, shaking tables and other devices, therefore, the investment is small; no sterile air is introduced, therefore, the operation is convenient; and no energy is consumed, as a result, the invention is energy-saving and environment-friendly.

The invention relates to a preparation method of an aqueous phase non-toxic white light quantum with a multilayer core-shell structure. The preparation method comprises the following specific steps: (a) preparing a shell layer material Zn+mercaptopropionic acid (MPA)+thioacetamide (TAA): taking Zn(NO3)2 and adding the Zn(NO3)2 into a conical flask filled with deionized water, taking the MPA by using a miniature liquid-transferring gun; adding the TAA into the solution; fully stirring the solution and then adjusting the pH value of the mixed solution by using NaOH; and fully stirring the solution for later use; (b) adding the deionixed water, manganese chloride and the MPA in a three-necked bottle, adjusting the pH value and then introducing nitrogen gas and removing air in the three-necked bottle by using high-purity nitrogen gas; injecting sodium hydrogen selenide by using an injector and then allowing the manganese chloride and the sodium hydrogen selenide to be fully acted to form a selenide-rich MnSe quantum dot; then injecting zinc nitrate and growing for one hour; injecting the shell layer material in the step (a) and growing; then adding a copper chloride solution and growing; adding the shell layer material; and finally, regrowing to obtain the required white light quantum dot. The white light quantum dot synchronized by the preparation method is better in water solubility and stability and has broad application prospect.

The invention relates to a method for detecting a residual free lithium content in a ternary anode material. The method comprises the following steps of in a closed conical flask, using a m1g ternaryanode material and m2g boiled distilled water to prepare a solution; using a nitrogen gas to carry out air emptying in a bottle; carrying out magnetic stirring at a room temperature and standing; using a needle filter to rapidly filter the solution into a collecting bottle so as to prepare a liquid to be tested; taking the m3g liquid to be tested to mix with the boiled distilled water for dynamicpotentiometric titration, and recording volumes V1 and V2 corresponding to a jump point; and calculating contents of Li2CO3 and LiOH through using the following formulas: WLi2CO3=CHCl*(V2-V1)*73.89 / 1000 / (m1*m3 / m2)*100%; and WLiOH=CHCl*(2V1-V2)*23.941 / 1000 / (m1*m3 / m2)*100%. By using the method for detecting the residual free lithium content in the ternary anode material, an error of a detection result is small, precision is high, and an interference condition during a testing process can be effectively determined.

Provided is a method for measuring copper content in tin-silver-copper solder through iodometry. The method comprises the following steps: a copper standard solution is prepared and the titer of the copper standard solution is measured. A sample to be measured is weighed and added into an Erlenmeyer flask. Concentrated sulfuric acid is added and the mixture is heated for dissolution. The above solution is cooled to the room temperature, perchloric acid is added, after the sample is dissolved fully, heating is carried out until white smoke is generated, concentrated hydrochloric acid is dropwise added into the above solution in batches to remove the tin element in the solution, and the solution is subjected to concentration. The Erlenmeyer flask is taken down and cooled to the room temperature, deionized water is added, the constant volume is 100mL and the above solution is shaken up. Then an ammonium hydroxide solution is added and copper ammonia complex ions are formed. Then ammonium bifluoride is added into the solution and stirred until the blue color disappears. The above solution is cooled to the room temperature by utilization of running water. The solution is placed for half a minute, then potassium iodide is added, and immediately the solution is subjected to titration by a sodium hyposulfite standard solution until a shallow yellow color appears. Then a potassium thiocyanate solution and a starch solution are added, and the solution is subjected to titration by the sodium hyposulfite standard solution until a blue color disappears. The volume of the consumed sodium hyposulfite solution is recorded, and the content of copper in the sample is calculated.

The invention belongs to the technical field of purification of drinking water source water and governance of organic pollutants, and in particular relates to a preparation method for modified zeolite adsorbent and application. The concrete method comprises the following steps: firstly, screening, cleaning, drying, crushing and sieving natural zeolite for standby; secondly, preparing alkyl halide pyridine solution with certain concentration for standby; thirdly, quantitatively weighing a plurality of portions of standby zeolite and putting the zeolite into a conical flask, transferring modifier stock solution according to different solid-to-liquid ratios, reacting the zeolite with the modifier stock solution for certain time at certain temperature and certain oscillating speed, then standing the mixture to carry out solid-liquid separation, and cleaning the product by water till a modifier cannot be detected out; and finally, drying the product at certain temperature to obtain a modified zeolite adsorbent product. The adsorbent which is applied to adsorbing and removing organic substance humic acid and the like in water has good effect. The method realizes efficient utilization of cheap natural resources; and the prepared product is suitable for removing the organic substance humic acid and the like in the water of water sources with high organic substance content, and has the advantages of high utilization rate, low cost and the like.

The invention relates to a method for testing vanadium in vanadium-nitrogen alloy. The method has the following steps: crushing and sieving the test sample through 200 mesh, fetching the sample from the erlenmeyer flask, adding H2SO4(1+4) and dissolving it by heating, dropping nitric acid to oxidizing it, adding concentrated H3PO4 and heating to the state that the sulfuric acid emits smoke, and then cooling to room temperature; adding (NH4)2Fe(SO4)2 standard solution, dropping KMnO4 solution till the test solution is red steadily and the color does not fade; adding urea solution, dropping NaNO2 solution till the red color fades, adding NaAsO2 solution, placing, dropping 2-3 drops of CrV indicator, immediately titrating with the ammonium ferrous sulphate standard solution till the color changes from cerise into light green, according to the volume of the consumed ammonium ferrous sulphate standard solution, calculating the content of the vanadium in the sample. The test result of the vanadium in vanadium-nitrogen alloy is accurate and the method is convenient.

A clamp for an Erlenmeyer flask or other laboratory containers or racks uses nickel-coated, rare earth magnets to secure the clamp to a platform for a laboratory shaker. The base of the clamp has downwardly extending positioning bosses that seat in holes or indentations on the shaker platform to prevent horizontal sliding of the clamp when the shaker is in use. A removable and replaceable, elastomeric cover for the base of the flask clamp provides cushioning and prevents spinning of the flask when the shaker is in use.

The invention discloses a method for regulating the adsorption of heavy metals in a single system by using carbon nanomaterials. It is to weigh 50 mg of carbon nanomaterials into a 150 ml Erlenmeyer flask, add 100 ml, 100 mg·L ‑1 Cu 2+ , Pb 2+ 、Cd 2 Single ion solution, adjust the pH to 6, shake at room temperature for 1 min, 3 min, 5 min, 10 min, 15 min, 30 min, 1 h, 2 h, 4 h, 8 h, 24 h, and filter the solution quickly , The residual concentration of metal ions in the filtrate was determined by atomic absorption spectrophotometry. The invention further discloses the application of the method for regulating the adsorption of heavy metals in a single system by carbon nanomaterials in improving the adsorption speed of heavy metals. Among them, the adsorption affinity is strong for carbon nanotubes and Cd, strong for graphene oxide and Cu, and strong for graphene and Pb.

The invention discloses a method for preparing a gamma-poly glutamic acid bioflocculant by bacillus licheniformis, relates to a bioflocculant, and provides a method for preparing the gamma-poly glutamic acid bioflocculant by the bacillus licheniformis. The method comprises the steps of transferring the bacillus licheniformis to a slant culture medium to cultivate; choosing an activated strain by an inoculating loop; inoculating into a conical flask with seed culture medium to cultivate; inoculating into the conical flask with seed culture medium to cultivate; taking a secondary seed, inoculating into a fermentation culture medium to cultivate by 3% of inoculating quantity; centrifuging bioflocculant fermentation liquor, removing the sediment, collecting upper clear liquid; adding ethanol to the upper clear liquid and then stewing, centrifuging and removing the upper clear liquid; dissolving the sediment into deionized water; dialyzing by a dialysis bag; adding absolute ethyl alcohol to the dialyzed solution, evenly agitating, centrifuging and removing the upper clear liquid; freezing and drying the sediment in vacuum to obtain pure gamma-poly glutamic acid bioflocculant. The method is short in fermentation cycle, high in activity of synthetic flocculant, good in thermal stability, and large in industrial application potential.

The invention discloses a water purifying agent with quick result, which comprises the following bacterial strains by weight: 1-4 of bacillus subtilis; 1-4 of nitrifying bacteria; 1-4 of lactobacillus plantarum; 1-4 of rhodopseudomonas palustris. The bacterial strains of bacillus subtilis, nitrifying bacteria, lactobacillus plantarum, rhodopseudomonas palustris and the like are cultured by the following processes of: 1. bacterial strain preservation; 2. slant culture in test tubes; 3. liquid culture in erlenmeyer flasks; 4. liquid culture in seed tanks; 5. solid inoculation and fermentation; 6. low-temperature drying; 7. pulverization; 8. examination for qualification; 9. packaging to keep in reserve. The microbial water purifying agent of the invention is mainly used to decompose excreta of aquatic animals, unconsumed feed, remains of animals and plants, organic dirt in pond sediments, and harmful substances of ammonia nitrogen, hydrogen sulfide, nitrite and the like in water, and synthesize beneficial substances which can be absorbed by beneficial algae so as to ensure excellent water quality in the middle and late period of culturation.

A clamp for an Erlenmeyer flask or other laboratory containers or racks uses nickel-coated, rare earth magnets to secure the clamp to a platform for a laboratory shaker. The base of the clamp has downwardly extending positioning bosses that seat in holes or indentations on the shaker platform to prevent horizontal sliding of the clamp when the shaker is in use. A removable and replaceable, elastomeric cover for the base of the flask clamp provides cushioning and prevents spinning of the flask when the shaker is in use.

The invention especially relates to a method for extraction and content determination of loganin, belonging to the technical field of determination methods. The method provided by the invention has the advantages of simple operation and accurate determination. According to a technical scheme in the invention, the method comprises the following steps: weighing loganin and adding methanol to prepare a loganin solution with a concentration of 60 mu g / ml; weighing to-be-determined powder, sieving the powder with a sieve No. 3, placing the sieved powder into a cone flask with a plug, adding 80% methanol, carrying out ultrasonic treatment for 30 min, then carrying out centrifugation for 20 min, taking a supernatant and subjecting the supernatant to microporous membrane filtration; taking a loganin reference solution and carrying out sample introduction 3 to 10 times, wherein a peak area RSD is 0.84%; weighing medicinal powder and calculating loganin content, wherein RSD of the medicinal powder is 1.07%; and weighing 6 parts of the medicinal powder with known content, putting the medicinal powder into a round bottom flask, adding the loganin reference solution and carrying out determination.

The invention belongs to the technical field of radioactive substance impact assessment, and relates to a method for polonium purification and source preparation in aerosol. The method sequentially comprises: (1) transferring a pre-treated polonium sample to be determined into an erlenmeyer flask, and adding <208>Po(IV) or <209>Po(IV) with a certain activity as a tracer to obtain a polonium sampleto be subjected to purification and source preparation; (2) placing the polonium sample obtained in the step (1) in a water bath, evaporating to remove more than 90% of the liquid, and cooling to a room temperature; (3) adding a hydrochloric acid solution, a hydroxylamine hydrochloride solution and ascorbic acid to the sample solution obtained in the step (2), mixing, and uniformly shaking; (4) adding the sample solution obtained in the step (3), and a polished silver sheet or copper sheet to a device capable of providing a certain sealing effect and a certain condensation recovery effect; (5) after installing the device, placing into an oscillator, and carrying out constant temperature water bath oscillation; and (6) after carrying out oscillation for a certain time, taking the poloniumplated silver sheet or copper sheet out of the device, rinsing with distilled water, and carrying out air drying. With the method of the present invention, the recovery rate of the purification and source preparation can be increased and stabilized so as to improve the accuracy of the <210>Po determination result.

The invention provides a method for improving output of an anticancer anthraquinone compound 1403C generated by Fusarium proliferatum (No.1403) via utilizing different vaccination ways. The method comprises the following steps: a conical flask with a baffle is adopted to cultivate seeds for grinding inoculation, the seeds cultivated by the conical flask with the baffle are directly inoculated, and seeds cultivated by glass beads are added into the conical flask, therefore, the 1403C yield obtained by fermentation is improved to different degrees compared with the yield obtained by fermentation for cultivating seeds (control group) in a traditional inoculation way, wherein the maximum 1403C yield is 1.13g / L, which is improved by 289.7% compared with 0.29g / L yield of the control group. The invention has vital significance for further performing 1403C anti-cancer pharmacological effect experiments.

The invention provides an analysis method for polyether polyol hydroxyl value, which comprises the following steps: A, weighing a polyether polyol test sample m into a conical flask, and taking another empty conical flask; B, transferring a phthalic anhydride pyridine solution into the test sample accommodated conical flask and the empty conical flask respectively, placing the two conical flasks in an oscillation oil bath pan, and connecting the two conical flasks with an air condensation pipe for reaction; C, after reaction, taking the two conical flasks out of the oil bath pan for cooling; D, flushing the two conical flasks with deionized water and acetone in sequence, and oscillating the two conical flasks to mix the solutions completely; E, adding a sodium hydroxide aqueous solution into the two conical flasks respectively, and adding a few drops of phenolphthalein indicator; F, titrating the solutions in the two conical flasks with potassium hydroxide standard solution until the solutions in the two conical flasks changes into pink; and G, calculating out the polyether polyol hydroxyl value as per a formula. The method is simple in analysis procedure, has high precision and accuracy, can efficiently shorten detection period, lower experimental cost, and bring certain economical benefits to an enterprise.

The invention relates to a test method of material characteristic, in particular to a test method for expansion ratio of a cement emulsified asphalt mortar, which solves the problem of poor repeatability and high variation coefficient of a measuring result of the measuring cylinder height variation method and the like. The method comprises the following steps: erecting a measuring cylinder on a horizontal plane; calibrating a volume V0 of the measuring cylinder and the volume V of an erlenmeyer flask; injecting the cement asphalt mortar into the erlenmeyer flask, making the mortar surface be flush with the flask mouth, and recording the mass m of the mortar; obtaining an apparent density rho from the volume V of the erlenmeyer flask and the mass m of the mortar; putting the measuring cylinder on a balance to deduct the mass of the measuring cylinder, injecting part of the mortar in the erlenmeyer flask into the measuring cylinder, and recording the mass m0 of the mortar; calculating an initial volume V1 of the mortar injected into the measuring cylinder; putting the measuring cylinder on the balance to deduct the mass of the measuring cylinder after 24 hours, filling the measuring cylinder with water, recording the mass m1 of the water added, and calculating a volume V2 of the water added; and the expansion ratio (percent) can be obtained through a formula of ((V-V1-V2) / V1)*100%. The repeatability of the measuring result is high, the range, the standard deviation and the variation coefficient of the measurement data are less than that of the measuring cylinder height method, and the operation is simple.

The invention relates to a method for fast and effectively improving the component measurement precision of twelve elements in stainless steel. The method is characterized in that plasma gas is connected with a plasma emission spectrometer by a pipeline; auxiliary gas is accessed into a middle layer of a plasma torch tube of the plasma emission spectrometer; an atomizer is arranged in the plasma emission spectrometer; the working parameters of the plasma emission spectrometer such as inductance coupling whole spectrum direct reading and the like are confirmed; the analysis line of each element is confirmed; 5 to 10 standard steel samples or mixed standard working solution similar as an analysis sample are selected as a standard working curve; the sample is put into an Erlenmeyer flask and is put into the plasma emission spectrometer after nitric acid and hydrochloric acid are added, and then is connected with the atomizer; liquid sample is transformed into mist state to be sprayed into an atomizer chamber and reach a plasma torch; and the contents of elements manganese, silicon, phosphor, chrome, nickel, molybdenum, copper, cobalt, columbium, vanadium, aluminium and titanium in the sample are mensurated at the same time. The invention has the advantage that all components of every measured element reach high precision.

The invention discloses a method for determining available trace elements in soil. The method comprises the following steps: (1) picking out the animal and plant residues, stone and the like after air drying the soil sample, pouring the sample into a clean wooden tray, and porphyrizing the sample by a stick to make the sample all pass the 2mm nylon mesh; (2) weighing a certain amount of soil sample to place in a conical flask with cover, adding a certain amount of extracting agent, stirring the mixture, and performing graded ultrasonic extraction for 5-30 min; and (3) filtering or centrifugating the content in the conical flask, taking the supernatant to determine the content of available iron, manganese, copper, and zinc by using inductively coupled plasma mass spectrometry. The method is based on the supersonic wave having rapid and high-efficiency extraction technology, combines the characteristics of the inductively coupled plasma mass spectrometry such as high sensitivity, wide linearity range, and multielement simultaneous determination, and has very important meaning of researching available trace elements in agricultural soil. The invention invents the method for analyzing available trace elements in soil efficiently and rapidly.

The invention relates to an analytical determining method for a chemical phase of a vallerite in a copper sulfide ore. The analytical determining method comprises the following steps: weighting two ore samples same in weight; placing a first ore sample into a conical flask, adding 1%-2% silver nitrate and 4-6mol / L ammonia solution into the reaction vessel, performing leaching treatment in boiling water bath, and then determining a content of a mixture of copper oxide and secondary copper sulfide and recording the content as Cu I; placing a second ore sample into a beaker, adding 8%-12% hydrochloric acid and 120-200g / L thiourea solution, continuously stirring and leaching, determining at constant volume after leaching, and marking the content of the mixture of copper oxide, secondary copper sulfide and vallerite in the sample as Cu II; and obtaining a result according to a formula: Cu vallerite equal to Cu II minus Cu I. According to the analytical determining method disclosed by the invention, two samples instead of one sample are adopted, so that the influence of silver in leached residues in subsequent leaching after the copper oxide and the secondary copper sulfide are leached by using ammonia and silver nitrate on vallerite leaching is eliminated, the accuracy of the result is increased, and the problems of bigger error, long flow and low efficiency in traditional separating determination are solved.

The invention discloses a preparation method of a silver nanoparticle with a controllable particle size. The preparation method comprises the following steps that (1) silver nitrate and a surface active agent are dissolved in glycol to be prepared into a solution A, and sodium chloride is dissolved in deionized water to be prepared into a solution B; (2) the solution A and the solution B are mixed to be used as a reaction solution and transferred to a conical flask for microwave irradiation reaction, so as to obtain the silver nanoparticle, and the diameter of the silver nanoparticle can be controlled between 100-250 nm by controlling reaction conditions. The preparation method has the characteristics of easiness in obtaining raw materials, simple equipment, low cost, simplicity in operation, high efficiency, rapidity and the like, the prepared silver nanoparticle has the advantages of regular shape, uniformity in particle size distribution, good dispersibility, controllability in size, high yield and good stability and is suitable for large-scale industrial production. The silver nanoparticle prepared by using the preparation method increasingly shows an important application value in the fields of microelectronics, photoelectron, catalysis, magnetic devices, information storage, medicine and the like, and the preparation and the performance research become hot spots.

The invention discloses a preprocessing method for testing abundance of carbonates sample 14 C. The preprocessing method comprises the steps of transferring carbon atoms in carbonate sample to CO2 molecule by enabling carbonate to react with an acid solution, enabling CO2 to react with a NaOH solution to obtain a saturated NaHCO3 solution, heating the saturated NaHCO3 solution to obtain a saturated Na2CO3 solution, and using the saturated Na2CO3 solution to test the abundance of 14 C. The invention further discloses a device used for the method. The device mainly comprises a nitrogen cylinder, an absorption cold trap A, a piston A, a conical flask A, a conical flask B, a moisture absorption cold trap B, a three-way piston, a vacuum gauge, a vacuometer, a piston B, a vacuum pump and a U-shaped gas sample collector. The method is capable of greatly simplifying chemical processing process, improving time efficiency and reducing cost on the basis of ensuring the testing precision.

The invention relates to a biocontrol strain KMXU1 capable of preventing and treating blueberry blight and antibiological inoculant thereof and belongs to the technical field of biopesticide. Preservation number of the strain KMXU1 is CGMCC NO. 6597. The antibiological inoculant is obtained by the following steps of: transplanting the strain KMXU1 into a test tube, carrying out one-cycle activation, and culturing for 48 hours at the temperature of 28 DEG C; transferring an activated bacterial lawn into 600ml of LB culture solution filled in a 1000ml conical flask, and culturing for 72 hours under the conditions that the rotating speed is 120r / min and the temperature is 28 DEG C, so that zymogeneous bacteria solution is obtained; inoculating the zymogeneous bacteria solution into the LB culture solution in a seed tank in the ratio of 1: 200 by inoculation amount, carrying out fermental cultivation under the condition that temperature of the seed tank is 29-31 DEG C, and fermenting for 16-18 hours, so that strains used for fermentation is obtained; inoculating the strains used for fermentation into a fermentation tank in the ratio of 1: 20 by the inoculation amount, carrying out the fermental cultivation under the condition that the temperature of the fermentation tank is 29-31 DEG C, fermenting for 72-96 hours, and storing the fermented strains as the antibiological inoculant; and centrifuging the antibiological inoculant at 5000rpm for 6 min, and preparing active inoculant with preservative fluid in the ratio of 1: 50 (g / ml). The invention has the advantages that cost is low, no position or pollution is produced, and biocontrol effect is good; and successive cropping obstacle of blueberry soil-borne disease can be effectively prevented, and fruit yield of blueberry is increased.

This invention relates to a method for producing L-lactic acid from Rhizopus oryzae by solid-state fermentation. The method comprises: (1) culturing the seeds in an inclined culture medium at 30-36 deg.C for 3-5 days, inoculating into an erlenmeyer flask or eggplant flask of sporulation culture medium, culturing at 28-36 deg.C for 70-90 h, and expanding culture according to the inoculation amount of the seeds to obtain Rhizopus oryzae spores with corresponding amount; (2) inoculating Rhizopus oryzae seeds into a fermentation tank loaded with 15-60 vol.% of solid-state fermentation culture medium, and culturing at 28-36 deg.C for 48-84 h. The himidity is 65-90%, and the aeration rate of the fermentation tank is 10-20 L / h. Compared with liquid-state fermentation, the solid-state fermentation has such advantages as no need for stirring, low energy consumption, low production cost, and high L-lactic acid quality.