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Method for producing rhodobacter sphaeroides microbial fertilizer from citric acid waste water

A technology of citric acid wastewater and Rhodobacter sphaeroides, which is applied in the field of microorganisms to achieve the effects of improving farmland environmental quality, huge social and economic benefits, and reducing production costs

Inactive Publication Date: 2011-09-14
无锡中科活力生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And take citric acid waste water as main medium raw material, the method for cultivating Rhodobacter sphaeroids has no bibliographical information

Method used

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  • Method for producing rhodobacter sphaeroides microbial fertilizer from citric acid waste water
  • Method for producing rhodobacter sphaeroides microbial fertilizer from citric acid waste water
  • Method for producing rhodobacter sphaeroides microbial fertilizer from citric acid waste water

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0027] The citric acid wastewater from the 3# pipeline of Jiangsu Yixing Xielian Biochemical Co., Ltd. was used as raw material. Wastewater COD is 18000mg / L.

[0028] Production steps:

[0029] a. Pretreatment of citric acid wastewater:

[0030] First dilute the citric acid wastewater with tap water to a COD concentration of 15000mg / L, then adjust the pH of the wastewater to 6.5-7.5 with a 12N aqueous sodium hydroxide solution, and sterilize at 121°C for 20 minutes.

[0031] b. Liquid seed preparation:

[0032] Inoculate the Rhodobacter sphaeroides preserved on the slant into the Erlenmeyer flask containing the above-mentioned pretreated citric acid wastewater, and culture it at 32° C. with shaking at 150 rpm for 30 hours to obtain liquid seeds.

[0033] c. Liquid fermentation:

[0034] Inoculate 10% (volume ratio) liquid seeds in a fermenter equipped with pretreated citric acid waste water, and cultivate in sterile air at 32°C for 30 hours.

[0035] d. Post-processing: ...

preparation Embodiment 2

[0038] The citric acid wastewater from the 1# pipeline of Yixing Xielian Biochemical Co., Ltd. was used as raw material, and the COD of the wastewater was 21000mg / L.

[0039] Production steps:

[0040] a. Pretreatment of citric acid wastewater:

[0041] First dilute citric acid wastewater with well water to a COD concentration of 14000mg / L, then adjust the pH of the wastewater to 6.5-7.5 with 18N sodium hydroxide aqueous solution, and sterilize at 116°C for 30 minutes.

[0042] b. Liquid seed preparation:

[0043] Inoculate the Rhodobacter sphaeroides preserved on the slant into the Erlenmeyer flask containing the above-mentioned pretreated citric acid wastewater, and culture at 35° C. with shaking at 180 rpm for 40 hours to obtain liquid seeds.

[0044] c. Liquid fermentation:

[0045] Inoculate 15% (volume ratio) liquid seeds in a fermenter equipped with pretreated citric acid waste water, and cultivate in sterile air at 35°C for 36 hours.

[0046] d. Post-processing:

...

experiment example 1

[0049] The comparison experiment was carried out by using the rhodobacter sphaeroides microbial fertilizer and urea obtained in Preparation Example 1 as fertilizers.

[0050] Using the vegetable greenhouse plot experiment, set up 12 experimental plots with exactly the same conditions, each plot 60m 2, planting rapeseed, in the rapeseed seedling stage, keep 3 non-fertilizing plots; 3 plots use 50L / mu of fertilizer after the rape is completely germinated and after 3 weeks, respectively apply Rhodobacter sphaeroides microbial fertilizer once; 3 plots use 15kg The amount of fertilization per mu was applied urea once after the complete germination of rapeseed and 3 weeks later; urea was applied once respectively after the complete germination of rapeseed and 3 weeks later according to the fertilization amount of 15kg / mu, and 1 time after each application of urea. The microbial fertilizer was applied once a day according to the fertilization rate of 50L / mu, and the same irrigation a...

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Abstract

The invention discloses a method for producing a rhodobacter sphaeroides microbial fertilizer from citric acid waste water, and belongs to the microorganism field. The method comprises the following steps: a. diluting citric acid waste water with clear water till the COD concentration is 12000-15000 mg / L, adjusting the pH to 6.5-7.5 with a sodium hydroxide aqueous solution, sterilizing the solution at 115-126 DEG C for 10-35 min; b. inoculating rhodobacter sphaeroides preserved by a slant into an erlenmeyer flask containing the citric acid waste water, performing shaking culture at 30-37 DEG C under 120-200 rpm for 24-48 hours to obtain liquid seeds; c. inoculating the liquid seeds into a fermentor containing the citric acid waste water with a volume ratio of 2%-20%, culturing the seeds in sterile air at 25-37 DEG C for 12-48 hours to obtain fermentation broth; d. determining the cultured fermentation broth by a spectrophotometer, packaging the broth when the OD660 value is 1.0-1.5 orthe bacterial count reaches 109-1010 CFU / ml so as to obtain a finished product. Compared with the prior art, the invention produces a microbial fertilizer from citric acid waste water, and has the characteristics of low production cost, energy saving and environmental protection, good product application effect, etc.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a method for producing rhodobacter sphaeroides microbial fertilizer by utilizing citric acid wastewater. Background technique [0002] Citric acid is widely used as an important chemical raw material and food additive. Its production is based on dried potato or corn as raw material, followed by raw material treatment, fermentation, extraction, refining and other processes to obtain products. Citric acid wastewater mainly comes from the neutralization solution, sugar washing water, tank washing water and filter cloth washing water produced in the fermentation and extraction process. The COD concentration is mostly 15000-25000mg / L, which belongs to high-concentration organic wastewater. According to statistics, for every ton of citric acid produced, 7.5 to 15 tons of high-concentration wastewater will be produced. Direct discharge will cause serious pollution to the environment, and the co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F7/00C05F11/08
CPCY02A40/20
Inventor 王栋白志辉吴美君李磊张艳曹荧凌德霞王诚
Owner 无锡中科活力生物技术有限公司
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