14 results about "Rhodobacter sphaeroides" patented technology

The application provides a coenzyme Q10-producing genetically engineered bacterium (Rhodobacter sphaeroides), and the preservation number is CGMCC NO.19600. The application also provides application of the coenzyme Q10-producing genetically engineered bacterium CGMCC NO.19600 in fermentative production of coenzyme Q10. The coenzyme Q10-producing genetically engineered bacterium constructed by the application can greatly improve the yield of coenzyme Q10, and has a wide industrial application prospect.

The invention discloses an artificially synthesized corrinic acid operon, and the sequence of the artificially synthesized operon is obtained by integrating corrinic acid synthesis related genes and connecting and constructing the corrinic acid synthesis related genes in a reordering and end-to-end manner. According to the operon, a recombinant strain is synthesized on the basis of various chassis strains such as escherichia coli and rhodobacter sphaeroides, the corrinic acid and the derivatives thereof are synthesized through fermentation, the corrinic acid and the derivatives thereof can also be synthesized through a cell-free catalytic system, the problem that overlapping genes of a natural operon are difficult to modify is solved, and the synthesis efficiency and controllability of the corrinic acid are improved.

The invention relates to the technical field of microorganisms, in particular to a compound microbial agent as well as a preparation method and application thereof. The invention provides a compound microbial agent. The effective components of the compound microbial agent are fermentation liquor of rhodobacter sphaeroides GTW-A1 and fermentation liquor of bacillus licheniformis GTW-B2, the preservation number of the rhodobacter sphaeroides GTW-A1 is CGMCC (China General Microbiological Culture Collection Center) No.33609, and the preservation number of the bacillus licheniformis GTW-B2 is CGMCC No.33610. The invention further discloses a preparation method of the The compound microbial agent can effectively increase the carbon reserve of soil, passivates the activity of heavy metals through the synergistic effect of bacterial strain metabolites, improves the soil structure and promotes ecological restoration of microorganisms.

The application provides a preparation method of a Rhodobacter sphaeroides CsCobT and CsCobN protein complex in vitro, and steps are as follows: constructing an expression vector pET-28a(+)‑Cs CobN , pET-28a(+)‑SUMO‑Cs CobT ; expressing and purifying His-CsCobN protein; preparing a non-tagged CsCobT protein; mixing the purified His-CsCobN protein and the non-tagged CsCobT protein to form a protein complex; separating the complex through affinity chromatography; and refining the complex through gel filtration chromatography. The application adopts a strategy of double vectors, step-by-step purification, in-vitro assembly and multi-dimensional verification, can obtain a CsCobT and CsCobN protein complex with high purity and uniform conformation, and the experimental process can be accurately controlled, the result is specific, and provides high-quality protein materials for subsequent molecular mechanism research and application development of cobalt-chelating enzyme.

The application provides a promoter library, a construction method of the promoter library and application of the promoter library. The promoter library of the application comprises promoters derived from Rhodobacter sphaeroides or modified variants thereof. The application discloses nucleic acid sequences of each promoter in the promoter library, vectors containing the nucleic acid sequences of the promoters and host cells. The application also discloses a screening method of the promoter library, and a promoter library with a gradient distribution of strength is obtained, so that high expression or low expression of a target gene can be realized and / or fine adjustment of expression thereof can be realized.

The invention discloses recombinant Saccharomyces cerevisiae for producing decaisopentenol as well as a construction method and application of the recombinant Saccharomyces cerevisiae, and belongs to the technical field of microorganisms. The preparation method comprises the following steps: expressing a decaisopentenyl pyrophosphate synthase gene from Rhodobacter sphaeroides in saccharomyces cerevisiae, and carrying out fermentation on the decaisopentenyl pyrophosphate synthase gene to obtain the decaisopentenyl pyrophosphate synthase. Overexpressing the endogenous phosphatidic acid phospholipase gene of the saccharomyces cerevisiae; the recombinant saccharomyces cerevisiae is obtained in a manner of reducing the transcriptional level of a squalene synthesis gene ERG9 and strengthening an MVA endogenous way. According to the method, the production of the decyl isopentenol is realized in the saccharomyces cerevisiae for the first time, the shake flask yield reaches 112mg / L, then the recombinant saccharomyces cerevisiae is fermented to extract the decyl isopentenol, and then the coenzyme Q10 is synthesized by a chemical method, compared with the traditional method for producing the coenzyme Q10 by fermenting the rhodobacter sphaeroides, the method has the advantages of high fermentation stability, simple culture medium and the like, and the method is suitable for industrial production. The industrial application potential is realized.

The application discloses a method for producing hydrogen through Clostridium pasteurianum-Rhodobacter sphaeroides coupling fermentation based on nitrogen limitation and application thereof. In the method, activated Clostridium pasteurianum is subjected to dark fermentation for producing hydrogen in a nitrogen-free culture medium and in an argon environment; then, the pH value of the obtained culture solution is adjusted to 7.0-8.0, the activated Rhodobacter sphaeroides is inoculated after selective sterilization, and the light fermentation for producing hydrogen is carried out in the argon environment. In the application, the fermentation solution of Clostridium pasteurianum does not need to be subjected to centrifugal removal of bacterial bodies and the like, and only needs to be simply adjusted in pH value, so that the light fermentation for producing hydrogen of Rhodobacter sphaeroides can be directly carried out. In the application, the nitrogen limitation is used to inhibit excessive proliferation of bacterial bodies, save carbon source flow, induce high expression of nitrogen fixation enzyme, and open a second high-efficiency hydrogen production path in addition to hydrogenase, so that the hydrogen production yield is further improved while the cost of nitrogen source is reduced. The hydrogen production yield of the application reaches 9.81 mol H2 / mol glucose, which is the highest level at present.

The application discloses a mutant strain with high coenzyme Q10 yield, and has a preservation number of CGMCC No.22200.The mutant strain is obtained by enrichment, separation, mutagenesis and screening of Rhodobacter sphaeroides with high coenzyme Q10 content.The application also discloses application of the mutant strain in production of coenzyme Q10 and in medicines, health products, cosmetics and food.

The application discloses a method for improving acid aluminum tolerance of sugarcane by high-efficiency combined nitrogen fixation, and relates to the technical field of sugarcane cultivation. The method comprises the following steps: controlling the nitrogen fertilizer amount, that is, only the bottom fertilizer applied in the whole growth period of the sugarcane contains the nitrogen fertilizer, and the fertilizer applied in other periods does not contain the nitrogen fertilizer; constructing a high-efficiency combined nitrogen fixation system, that is, applying the bacteria agent of Beijerinckia and the enhancer when the sugarcane is newly planted; applying the bacteria agent of Rhodobacter sphaeroides and the enhancer in the sugarcane seedling stage; applying the bacteria agent of Lipomyces and the enhancer in the sugarcane tillering stage; and the enhancer comprises indole acetic acid, eugenol and vitamin E. The method can make the sugarcane grow normally in the high-acid aluminum soil by controlling the nitrogen fertilizer amount, selecting suitable nitrogen fixation bacteria in different growth periods of the sugarcane and adopting a series of means such as the auxiliary use of the enhancer to construct the high-efficiency combined nitrogen fixation system.

The invention relates to an antibiotic-label-free inducible salt-tolerant rhodobacter sphaeroides engineering bacterium and application thereof, and belongs to the technical field of microorganisms. Wild rhodobacter sphaeroides is a dominant strain for biological sewage treatment, but natural strains grow very slowly when the salt concentration exceeds 3%, and the pollutant degradation capacity is obviously inhibited. Compared with wild strains and constitutive overexpression engineering bacteria, the inducible salt-tolerant rhodobacter sphaeroides engineering bacteria constructed by the invention have the advantages that the treatment capacity on ammonia nitrogen, phosphate and acetate in 3-5% high-salt sewage after lactose induction is obviously improved; technical support is provided for application of the rhodobacter sphaeroides in the fields of industrial wastewater purification in coastal areas, ecological restoration of saline-alkali soil, seawater recycling and the like, and the competitiveness of the rhodobacter sphaeroides in low-cost seawater culture, high-salinity wastewater treatment and special environment biological manufacturing is remarkably improved.

The invention belongs to the technical field of bioengineering, and discloses an expression strain of high-yield coenzyme Q10 and a production method of the coenzyme Q10. The expression strain of the high-yield coenzyme Q10, provided by the invention, is obtained by taking rhodobacter sphaeroides as an original strain and simultaneously carrying out gene modification construction of'crtI gene and crtB gene knockout 'and'GAP1 gene and / or UbiF gene overexpression'; through the combined action of multiple factors such as complete blocking of synthesis of carotenoid in cells, improvement of glycolysis efficiency, enhancement of reducing substance supply and enhancement of benzoquinone ring modification, directional reconstruction of metabolic flux for biosynthesis of the coenzyme Q10 in the rhodobacter sphaeroides cells is realized, so that the obtained expression strain is endowed with excellent coenzyme Q10 synthesis capability, and the expression efficiency of the rhodobacter sphaeroides is improved. Compared with an original strain, the yield of the coenzyme Q10 is increased, and the coenzyme Q10 has excellent application prospects in high-efficiency and low-cost industrial production of the coenzyme Q10.

The invention discloses application of rhodobryum harzianum in colorectal cancer diagnosis or prognosis, and belongs to the technical field of biomedicine.16SrRNA genome sequencing analysis finds that the content of rhodobryum harzianum in colorectal cancer tissue of a colorectal cancer patient is remarkably increased, the cancer promoting effect of the rhodobryum harzianum is further proved through a mouse AOM / DSS spontaneous tumor formation model, and the application of the rhodobryum harzianum in colorectal cancer diagnosis or prognosis is achieved. Compared with the prior art, the method provided by the invention has the advantages that the rhodobryum harzicola in a clinical sample is detected and a corresponding diagnosis model is established, so that the method has relatively high diagnosis efficiency and shows that the rhodobryum harzicola has important potential in clinical auxiliary diagnosis, treatment or prognosis evaluation of the colorectal cancer, a new way is provided for diagnosis, treatment or prognosis of the colorectal cancer, and the clinical application prospect is wide.

The invention provides a strain of bacillius pink and application thereof in promoting formation of sporangia branches of porphyra haitanensis. The preservation number of the provided bacillius pink is CCTCC (China Center for Type Culture Collection) NO: M 20242610. According to the method, the pink rod-shaped strain is added into a porphyra haitanensis protonema culture system, so that the development of the sporangium branches can be effectively promoted, the formation proportion of the sporangium branches can be remarkably increased to 48.8% after culture is performed for 4 weeks, directional ripening under manual intervention is realized, the defects of long period and poor stability of a traditional environment regulation and control means are overcome, and the method is suitable for large-scale popularization and application. The conchospore diffusion consistency and quality can be improved, and a new technical approach is provided for porphyra haitanensis seedling production.

The application discloses a recombinant saccharomyces cerevisiae for producing decaprenylphenol and a construction method and application thereof, and belongs to the technical field of microorganisms. Rhodobacter sphaeroides The recombinant saccharomyces cerevisiae is obtained by expressing a decaprenyl pyrophosphate synthase gene from Rhodobacter sphaeroides in the saccharomyces cerevisiae, overexpressing a phospholipase gene of endogenous phosphatidic acid of the saccharomyces cerevisiae, and down-regulating a transcription level of a squalene synthesis gene ERG9 and strengthening an endogenous MVA pathway. 10 The application realizes the production of decaprenylphenol in the saccharomyces cerevisiae for the first time, and the yield reaches 112 mg / L in a flask; decaprenylphenol is extracted from the recombinant saccharomyces cerevisiae through fermentation, and coenzyme Q 10 is synthesized through a chemical method, compared with traditional fermentation of Rhodobacter sphaeroides for producing coenzyme Q 10 , the method has the advantages of high fermentation stability, simple culture medium and the like, and has industrial application potential.