41 results about "Rhamnolipid" patented technology

This application discloses a composite oil displacement system and its preparation method, belonging to the field of oilfield development technology. The composite oil displacement system provided in this application includes rhamnolipin and betaine surfactant, wherein the rhamnolipin is a biosurfactant obtained by culturing *Pseudomonas aeruginosa*. Both the rhamnolipin biosurfactant and the betaine surfactant are heat- and salt-resistant; therefore, the composite oil displacement system prepared using the rhamnolipin biosurfactant and the betaine surfactant has good compatibility with high-temperature, high-salinity reservoirs, meaning it is suitable for such reservoirs. Therefore, it can significantly improve the oil displacement effect in high-temperature, high-salinity reservoirs.

This invention discloses a modified rhamnolipin waterproofing agent for well workover operations in offshore oilfields and its preparation method. The method involves a one-step process where a 3-chloro-2-hydroxypropyltrimethylammonium chloride aqueous solution is added dropwise to a rhamnolipin solution, attaching a quaternary ammonium cation to the hydroxyl group of the rhamnolipin. After the reaction is complete and the solution is purified, the modified rhamnolipin waterproofing agent is obtained. The raw materials used in this invention are readily available, the preparation method is simple, and it is conducive to industrial production. The raw materials and products used have low biotoxicity and good environmental friendliness, which is beneficial for application in offshore oilfields. The solution prepared with the modified rhamnolipin waterproofing agent exhibits low surface tension, good temperature and salt resistance, and wetting improvement ability. It can significantly improve the reservoir water-locking permeability recovery rate, demonstrating not only a good waterproofing effect but also alleviating reservoir contamination problems caused by long-term production to a certain extent.

The present application relates to a kind of coal slime collector preparation method using kitchen waste oil, comprising the kitchen waste oil after being pretreated with water, emulsifier is mixed, as carbon source configuration first culture medium, Bacillus and Rhodococcus species are inoculated in it and carry out aerobic fermentation, obtain refined oil, and as carbon source configuration second culture medium, Candida species are inoculated in it and carry out aerobic fermentation, obtain mixed fatty acid, and as carbon source configuration third culture medium, Yarrowia species are inoculated in it and carry out micro-aerobic fermentation, obtain alkane mixture;Emulsified oil and / or refined oil are as carbon source configuration fourth culture medium, Pseudomonas species are inoculated in it and carry out aerobic fermentation, obtain rhamnolipid;Alkane mixture is mixed with rhamnolipid, obtain coal slime collector.The present application develops a new type of green, good coal slime collector by constructing multistage series biological transformation process.

This invention discloses a self-thickening, moisturizing, and oil-controlling gentle facial cleanser and its preparation method, belonging to the field of cosmetic technology. The raw materials are composed of the following percentages by weight: rhamnolipin 2%~8%, alkyl glycoside 5%~15%, cationic polymer 0.1%~1.5%, moisturizer 2%~6%, plant extract 0.5%~3%, pH adjuster (the amount needed to adjust the system pH to 5.0~7.0), preservative 0.1%~1.0%, and water as the balance. Through the ternary system of rhamnolipin-alkyl glycoside-cationic polymer, self-thickening and foam stability are achieved through electrostatic interaction and steric hindrance synergy, without relying on traditional thickeners such as carbomer. Simultaneously, it possesses excellent moisturizing and oil-controlling properties, with an oil removal rate ≥89%, a skin moisture content increase of ≥15% after 4 hours of use, and an oil secretion inhibition rate of ≥20% after 4 hours, with extremely low irritation. This invention solves the technical problem of low viscosity and poor foam performance in the rhamnolipin and alkyl glycoside compound system.

A sulfate-free personal care composition that includes a rhamnolipid surfactant and provides good cleaning and foaming properties. The rhamnolipid surfactant has a single rhamnose head group (i.e., mono-rhamno) and a single lipid tail group (i.e., mono lipid). The single lipid tail group has a carbon chain length of 10 to 13. The composition may include one or more additional surfactants selected from anionic surfactants, amphoteric surfactants, zwitterionic surfactants, non-ionic surfactants, cationic surfactants and combinations thereof, as long as the additional surfactant is free of sulfates. The composition also includes a dermatologically acceptable carrier.

The application belongs to the field of admixtures, and particularly relates to a concrete thickening water-retaining agent and a preparation method and application thereof. The concrete thickening water-retaining agent is obtained by free radical polymerization reaction of an unsaturated acid monomer I, an unsaturated amide monomer, an unsaturated sulfonate monomer and a functional monomer; and the functional monomer is an esterification reaction product of rhamnolipid and an unsaturated acid monomer II. The key of the application is to introduce the esterification reaction product of rhamnolipid and the unsaturated acid monomer II as a copolymerization monomer into a copolymerization reaction system of the unsaturated acid monomer I, the unsaturated amide monomer and the unsaturated sulfonate monomer, so that the molecular structure of the concrete thickening water-retaining agent can be changed, the obtained concrete thickening water-retaining agent not only has excellent thickening and water-retaining properties, but also can improve the stability thereof in concrete.

The technology presented herein, in general, relates to the use of biosurfactants, such as rhamnolipids, for the treating wounds. More particularly, the present technology relates to a method for reducing or inhibiting the development of biofilm during to promote wound closure, by applying a composition comprising a mixture of specific rhamnolipids to the wound.

The present application is suitable for the field of bioremediation technology, and provides a bioremediation preparation for heavy oil pollution, which comprises an immobilized heavy oil degrading bacterial population preparation, an immobilized laccase preparation, a slow-release nitrogen and phosphorus nutrient preparation, and a rhamnolipid surfactant; the bioremediation preparation is a solid preparation. The present application also provides application of the bioremediation preparation in a heavy oil pollution intertidal zone. The bioremediation preparation has the advantages of not being easy to lose, fast bioremediation starting speed, being superior in competition with indigenous bacterial species, and good environmental tolerance, thereby overcoming the problems of slow starting speed and fast failure speed of traditional bioremediation preparations in remediation of heavy oil pollution intertidal zones.

The technology presented herein, in general, relates to the use of biosurfactants, such as rhamnolipids, for treating and / or accelerating the healing of wounds. More particularly, the present technology relates to a method for treating chronic and acute wounds, by applying a composition comprising a mixture of rhamnolipids to the wound, wherein application of the composition facilitates coordinated gene expression associated with wound healing.

This invention discloses a conditioner for purple soil contaminated with petroleum hydrocarbons, its preparation and application method, belonging to the field of purple soil remediation technology. The preparation method of the conditioner is as follows: urea solution is added to porous biochar or zeolite powder and stirred to allow the urea solution to enter the micropores of the porous biochar or zeolite powder, followed by drying to obtain a solid slow-release nitrogen source; the porous biochar and slow-release nitrogen source are mixed evenly, and then a mixed solution of rhamnolipin and humic acid is sprayed in, with a mass ratio of rhamnolipin to humic acid of (8-15):(2-5), so that the moisture content of the resulting material is 20-25%, stirred evenly, and finally dried until the moisture content is less than 3%, to obtain the conditioner for purple soil contaminated with petroleum hydrocarbons. Through synergistic formulation design, the physical improvement of soil structure and chemical / biological desorption of locked petroleum hydrocarbons are achieved, thereby realizing the synergistic effect of chemical desorption and microbial degradation.

The application discloses a kind of biosurfactant compound foam oil displacement agent and its preparation method and application, belong to the technical field of oil exploitation.The foam oil displacement agent is composed of the following components by mass percentage: rhamnolipid 15~35%, sophorolipid 10~25%, functional additive A 5~15%, functional additive B 3~10%, synergistic agent 2~8%, and the balance is deionized water to 100%;The mass ratio of rhamnolipid and sophorolipid is 1.3~1.5:1.The application also provides the preparation method and application of the oil displacement agent.The foam oil displacement agent of the application has the characteristics of large foaming volume, long foam half-life, excellent temperature resistance and salt resistance, high biodegradation rate, mild and simple preparation process, and is suitable for three times oil recovery in reservoir with temperature ≤120℃, salinity ≤30×10 4 mg / L, which can significantly improve the oil recovery rate and meet the requirements of green oilfield construction.

The application discloses a hair loss prevention shampoo targeting CD133 positive hair follicle dermal papilla cells and a preparation method thereof. The shampoo comprises a hair follicle targeting single-domain antibody-active peptide fusion, a liposome-encapsulated compound plant extract, a biological surfactant, a protein stability surfactant and an auxiliary material. The fusion is formed by fusing an anti-CD133 VHH single-domain antibody and a 5alpha-reductase inhibiting peptide DK-HP-7 through a flexible connecting peptide. Based on the discovery that CD133 positive dermal papilla cells are a key cell group driving hair follicle formation, the application uses single-domain antibodies to achieve precise targeting of active ingredients. The DK-HP-7 designed through molecular docking has an IC 50 of 4.28 μM. The rhamnolipid and Kolliphor HS 15 complex system can make the fusion protein activity retention rate higher than 89% after being stored at 40 ℃ for 3 months. The shampoo can significantly antagonize dihydrotestosterone-induced hair follicle growth inhibition, promote hair follicles to enter the growth phase and increase hair follicle density, and has good skin safety and stability.

This invention discloses a genetically engineered bacterium for producing rhamnolipids and its applications, belonging to the field of gene recombination and metabolic engineering technology. The genetically engineered bacterium for producing rhamnolipids disclosed in this invention uses *Yarrowia lipolyticis* ATCC MYA-2613 as the starting strain and heterologously expresses the rhamnolipid synthesis gene. rhlA, rhlB and rhlC, and dTDP-L-rhamnose synthesis gene rmlA, rmlB, rmlC and rmlD This invention discloses a genetically engineered bacterium for producing rhamnolipin, which can efficiently metabolize glucose or glucose-containing cellulose hydrolysates to produce rhamnolipin, achieving a rhamnolipin yield of 42.8 g / L. The genetically engineered bacterium constructed in this invention provides a possibility for the efficient production of rhamnolipin from glucose or glucose-containing cellulose hydrolysates.

The application provides a kind of edible composite preservative solution for low temperature refrigeration of fruit and vegetable, its preparation method and use method, the edible composite preservative solution includes phosphorylated nanocellulose solution, film forming agent, plasticizing agent, biological surfactant and curcumin / cyclodextrin inclusion compound;The film forming agent includes beeswax and / or coconut oil, the plasticizing agent includes glycerol and / or potassium sorbate, and the biological surfactant includes rhamnolipid and / or sophorolipid.The preservative solution provided by the application is composed of phosphorylated nanocellulose solution, film forming agent, plasticizing agent, biological surfactant and curcumin / cyclodextrin inclusion compound.Fruit and vegetable are directly soaked in the preservative solution or the preservative solution is sprayed on the surface of fruit and vegetable, and a complete and dense preservative coating is formed on the surface of fruit and vegetable after natural air drying, the oxygen concentration on the surface of fruit and vegetable is reduced by the respiration of fruit and vegetable, the carbon dioxide concentration is increased, and the controlled atmosphere preservation of fruit and vegetable is realized.

The present invention relates to the use of rhamnolipids to impart a feeling of mildness on the hands to consumers in surfactant systems for hand washing detergents.

The present application relates to a kind of oil displacement agent, in particular to a kind of hybrid modified multi-dimensional biological oil displacement agent and its preparation method.The hybrid polymeric biological surfactant of the present application is the product of esterification reaction of biological surfactant and polycarboxylic compound;The biological surfactant is at least one of lipopeptide, sophorolipid, rhamnolipid, sophorolipid modified derivative and rhamnolipid modified derivative;The polycarboxylic compound is at least one of oxalic acid, 1,3-propane dicarboxylic acid, butane tetracarboxylic acid, 1,3-butane dicarboxylic acid and polycarboxylic compound containing saturated alkyl chain with 18 carbon atoms or less between two carboxyl groups.The oil displacement agent provided by the present application has excellent temperature resistance, salt resistance, oil washing efficiency and imbibition oil displacement capacity.In particular, the oil displacement agent can be applied to extreme low permeability reservoir environment below 200 DEG C and below 200 g / L of salinity, and still maintain good chemical stability and interfacial activity under such harsh conditions.

The application discloses a ROS-targeted nano-drug delivery system, which uses a biomimetic hybrid nanovesicle and montmorillonite as a composite carrier and loads ROS-responsive micelles prepared by mixing a PD-L1 antagonistic peptide complex and an RGD complex. The biomimetic hybrid nanovesicle is prepared by modifying a cell-derived nanovesicle with a liposome composed of lecithin, cholesterol and rhamnolipid. The system realizes the synergy of four functions through the EPR effect of the biomimetic hybrid nanovesicle, the active targeting of RGD, the immunomodulation of the PD-L1 antagonistic peptide and the ROS-responsive rupture of the micelles. Experiments show that the system has a significant structural response under a high ROS environment, can quickly disintegrate to release drugs, has high selectivity and high uptake rate for high-ROS tumor cells, has low toxicity to normal cells and good blood compatibility. The system provides an efficient and safe nano-drug delivery platform for the targeted treatment of ROS-related diseases such as tumors.

This invention provides a fruit and vegetable preservative solution with strong wetting and ethylene inhibition effects, its preparation method, and its application method. The fruit and vegetable preservative solution comprises a nanocellulose solution, a film-forming agent, a plasticizer, a biosurfactant, and a 1-methylcyclopropene / cyclodextrin inclusion complex. The film-forming agent includes beeswax and / or coconut oil, the plasticizer includes glycerol and / or potassium sorbate, and the biosurfactant includes rhamnolipid and / or sophorolipid. This invention prepares a fruit and vegetable preservative solution with strong wetting and ethylene inhibition effects by encapsulating 1-methylcyclopropene with cyclodextrin and compounding it with nanocellulose, a film-forming agent, a plasticizer, and a biosurfactant.

This invention belongs to the technical field of PVC wall panel materials, specifically an antibacterial and mildew-resistant PVC wall panel material and its preparation method, comprising the following components: polyvinyl chloride resin, an antibacterial and mildew-resistant slow-release complex, a calcium-magnesium composite heat stabilizer, an environmentally friendly plasticizer, an acrylate processing aid, a composite lubricant, and fillers. This invention utilizes a composite coating system constructed with a rhamnose lipase-modified nano-zinc oxide amphiphilic coating layer as the inner layer and a phytic acid-modified β-cyclodextrin and trisodium citrate synergistic adsorption layer as the outer layer. This system encapsulates nano-zinc oxide within the PVC matrix, delaying the risk of zinc ion leaching from landfills and inhibiting the activity of key extracellular enzymes in the soil. Simultaneously, the heat stabilizer, plasticizer, and processing components help maintain the matrix's density, indirectly enhancing physical barrier properties and achieving low ecological impact throughout the wall panel's life cycle.

The application discloses a composite sewage treatment agent containing polyaluminum chloride and polyferric sulfate and a preparation method thereof. The composite sewage treatment agent is prepared from the following raw materials in parts by weight: 15-25 parts of polyaluminum chloride, 10-20 parts of polyferric sulfate, 10-18 parts of a composite microbial flora, 5-12 parts of an adsorption enhancer, 0.5-1.5 parts of rhamnolipid, and 2-5 parts of poly-gamma-glutamic acid. The composite sewage treatment agent can realize rapid and efficient sewage treatment and can meet certain long-acting treatment requirements.

This invention provides a method for separating and purifying rhamnolipin, which can efficiently separate rhamnolipin on a large scale at low cost and obtain a light-colored, low (or no) odorless aqueous solution of rhamnolipin. The method includes the following steps: (1) Filtration: The rhamnolipin fermentation broth is pre-filtered to remove large particles and obtain filtrate a; (2) Centrifugation: Filtrate a is centrifuged and the supernatant b is collected; (3) Adsorption treatment: Activated carbon is added to the supernatant b, and the temperature, time, pH and other conditions are controlled to complete the preliminary adsorption treatment. After centrifugation, the supernatant c is obtained; (4) Decolorization and deodorization: Diatomaceous earth with different particle sizes is added to the supernatant c, stirred and mixed, and decolorized and deodorized to obtain a mixed solution d; (5) Filtration: The mixed solution d is clarified by filtration to obtain a light-colored, transparent, low-odor clear solution, which is the finished product. The separation and purification process of this invention does not use organic solvents, the product is light-colored and transparent, and has a faint caramel smell.

This invention discloses a bud-promoting humic acid compound liquid fertilizer formulation and its preparation method suitable for cotton, relating to the field of agricultural fertilizer technology. The formulation, based on the preparation of 1000 parts by weight of the finished product, consists of the following raw material components in parts by weight: 700-750 parts by weight of water, 40-50 parts by weight of potassium hydroxide, 140-160 parts by weight of potassium dihydrogen phosphate, 80-100 parts by weight of urea, 65-75 parts by weight of mineral-derived potassium humate, 8-10 parts by weight of alkyl glycoside-rhamnolipid composite bio-penetrating agent solution, and polyaspartic acid- The fertilizer formula comprises 10-14 parts by weight of γ-aminobutyric acid (GABA) compound functional liquid, 15-25 parts by weight of mineral-derived potassium humate, 5-10 parts by weight of plant-derived compound amino acids, 3-6 parts by weight of EDTA chelated micro-fertilizer, 2-5 parts by weight of seaweed extract, and 1-3 parts by weight of polyethylene glycol-based wetting and spreading agent. This invention designs a fertilizer formula that meets the needs of cotton during the budding stage, achieving a dual function of nutrient supply and physiological regulation. A refined preparation process ensures the fertilizer is uniform and stable, suitable for large-scale production, and retains active ingredients, ensuring stable application effects.

This invention provides an ultrafiltration membrane for rhamnolipid separation and its preparation method. The method includes the following steps: preparing a core solution, an inner layer casting solution, an intermediate layer casting solution, and an outer layer casting solution; sequentially injecting the core solution, inner layer, intermediate layer, and outer layer casting solution into the corresponding channels of a multi-channel spinneret; curing the formed ultrafiltration membrane and then winding it into fibers; immersing the ultrafiltration membrane in a solution containing a hydrophilic additive to undergo chemical cross-linking and purification, thereby obtaining a hollow fiber ultrafiltration membrane. The cross-linking agent within the ultrafiltration membrane undergoes a cross-linking reaction with the hydrophilic additive, grafting hydrophilic compounds onto the surface of the ultrafiltration membrane, thereby improving the membrane's antifouling ability during concentration and its recovery performance after cleaning.

The application discloses a separation process of high-viscosity rhamnolipid under abnormal working conditions, which comprises the following steps: 1) adding soluble inorganic alkali into the high-viscosity rhamnolipid fermentation liquor, adjusting the pH to above 10, and standing for treatment; 2) adding normal primary alcohol into the rhamnolipid fermentation liquor after standing, and stirring for treatment; 3) centrifuging the fermentation liquor, and collecting supernatant; 4) filtering the supernatant by using an organic microfiltration membrane, and collecting filtrate; and 5) performing vacuum rectification on the filtrate, and obtaining rhamnolipid aqueous solution in a tower kettle. The separation process provided by the application is simple, the separation equipment used is all conventional equipment, and the separation of rhamnolipid and high-viscosity PHA can be well realized.

The invention discloses a high calcium and magnesium resistant bio-based nanofluid oil displacement agent. The oil displacement agent is prepared from TiO2 nanoparticles, a biological surfactant and an anionic-nonionic surfactant, the invention also discloses a preparation method of the high calcium and magnesium resistant bio-based nanofluid oil displacement agent. The preparation method comprises the following steps: adding TiO2 nanoparticles into deionized water, and stirring to obtain a TiO2 nanoparticle suspension; and sequentially adding the rhamnolipid solution and the anionic-nonionic surfactant solution into the TiO2 nanoparticle suspension, and simultaneously stirring and ultrasonically dispersing to obtain the nanofluid oil-displacing agent. According to the invention, the chemical surfactant and the bio-based macromolecules are coupled and co-connected to the surfaces of the nanoparticles, so that not only are the problems that a traditional nanoflow system is not resistant to high calcium and magnesium ions, is easy to settle and is relatively low in oil displacement efficiency solved, but also the problems that the chemical surfactant damages a stratum structure, is easy to form secondary environmental pollution and is complex in production process can be solved.