185 results about "Rhamnolipid" patented technology

The invention discloses a seed pelleting formula for improving salt tolerance of suaeda salsa in coastal saline-alkali soil and a processing method, the pelleting formula comprises 348-492 parts of a filler, 348-492 parts of a binder, 348-492 parts of a water-retaining agent and 348-492 parts of a biological stimulant, and the filler comprises attapulgite, talcum powder and vermiculite; the binder is 73-89 parts and comprises sodium carboxymethyl cellulose, polyvinyl alcohol and starch; the water-retaining agent accounts for 30 to 42 parts, and the water-retaining agent is polyacrylamide; the biological stimulant is prepared from the following components in parts by weight: 20-78 parts of yeast metabolite, potassium fulvate, seaweed essence, rhamnolipid and a microbial agent; the processing method comprises the steps of seed selection, raw material preparation, layered coating, low-temperature drying, selection and sieving, and polishing and shaping. According to the invention, a layered coating process is adopted to break through a traditional single mixed coating mode, an isolation breathable microenvironment is firstly constructed by the filling layer, then growth-promoting stress-resistant nutrient substances are injected by the active layer, and finally, the shaping layer is used for locking and shaping and strengthening protection, so that the seeding performance, salt tolerance, germination activity and seedling stress resistance of the pellets are remarkably improved.

The invention relates to microbial colorant compositions and associated processes to produce the same. Microbial colorant compositions are produced by combining microbially derived pigment molecules and microbial biosurfactants. The pigment molecules are obtained either directly from a fermentation broth or by extracting a precursor and transforming it into a pigment using chemical or enzymatic processes. A separately produced biosurfactant, such as a rhamnolipid or surfactin, is added to the obtained pigment molecule to create a pre-dispersed formulation. The biosurfactant may be added at various steps of This approach improves pigment dispersion, solubility, and substrate compatibility, enabling stable aqueous formulations without synthetic additives. The resulting compositions are suitable for wide range of applications including in textiles, food, cosmetics, and polymer materials. Processes for producing and combining the pigment and biosurfactant are also disclosed, including variations in fermentation, extraction, and formulation stages.

The invention discloses a Pseudomonas putida high-yield rhamnolipid fermentation medium based on a step-by-step optimization strategy, an optimization method and application thereof, through the step-by-step strategy of component screening-single-factor optimization-multi-factor interactive optimization, a mixed carbon source and a mixed nitrogen source are taken as cores, and a Plackett-Burman test, a steepest climbing test and Box-Behnken response surface design are combined, so that the high-yield rhamnolipid fermentation medium for the pseudomonas putida high-yield rhamnolipid is obtained. Optimizing to obtain a special fermentation culture medium; two genetic engineering strains estA-2 and estA-3 are constructed by heterologous expression of key genes, and the supply and extracellular transport efficiency of a rhamnolipid synthesis precursor is enhanced; the recombinant strain is applied to the optimized culture medium, and efficient production of a shake flask and a 5L fermentation tank is achieved. The rhamnolipid yield of the estA-3 strain in a 5L fermentation tank reaches 35.59 + / -2.18 g / L, the conversion rate is 0.282 g / g, the productivity is 0.404 g / L / h, the rhamnolipid yield, the conversion rate and the productivity are obviously superior to those in the prior art, and a key technical support is provided for industrial production of rhamnolipid.

The invention relates to pseudomonas putida engineering bacteria for producing rhamnolipid as well as a construction method and application of the pseudomonas putida engineering bacteria, the engineering bacteria take pseudomonas putida KT2440 with phaC1, phaC2, algD and fliC gene deletion as a chassis cell, and rhlAB, rmlACBD, algC and FabY genes are over-expressed in the chassis cell. Pseudomonas putida KT2440 with biological safety is used as a chassis cell for gene modification, the constructed engineering bacteria are used for fermentation production of rhamnolipid, on the basis of rhamnolipid yield increase, good physiological adaptability and fermentation stability are shown, and the rhamnolipid yield is increased. And the modified engineering strain can stably and efficiently produce rhamnolipid with a specific structure.

The invention provides a medium-low permeability oil reservoir rhamnolipid foam oil displacement system and a screening method and application, and relates to the technical field of microbial oil recovery, the medium-low permeability oil reservoir rhamnolipid foam oil displacement system comprises the following steps: S1, screening an applied oil reservoir; s2, determining a rhamnolipid foam flooding system based on the oil reservoir mineralization degree; s3, based on the porosity of the oil reservoir, the injection mode of a rhamnolipid foam oil displacement system is determined, the rhamnolipid foam oil displacement system comprises a foaming agent, a foam stabilizer, nitrogen and water, the foaming agent comprises one or more of dirhamnose difat, dirhamnose monofat, single rhamnose monofat and single rhamnose difat fermentation liquor, and the foam stabilizer comprises one or more of dirhamnose difat, dirhamnose monofat, single rhamnose monofat and single rhamnose difat fermentation liquor. The foam stabilizer is prepared from one or more of xanthan gum, welan gum, Wollan gum, Dide gum, gellan gum, rhamnose gum, diidine gum, plant cellulose and bacterial cellulose. The method has the beneficial effects that the oil reservoir application range is wide, the pertinence is high, the field test effect is good, efficient generation and long-acting migration of foam in the oil reservoir are guaranteed, green and high-quality development of old oil fields is promoted, and achievement of the national dual-carbon strategy is assisted.

The application discloses a kind of compound fertilizer with nitrogen fertilizer slow-release and biocontrol function and preparation method, it is related to microbial compound fertilizer field, including the following components: nitrogen source carrier, physical net trapping material, 3,4-dimethyl pyrazole phosphate, biocontrol component, binder.A kind of preparation method, including the following steps: after silanization modification treatment is carried out to attapulgite, with polyglutamic acid aqueous solution mixed reaction, obtain self-assembly gel;Bacillus subtilis is embedded and handled, rhamnolipid is pre-emulsified, chitosan oligosaccharide is crosslinked with boric acid treatment, and is blended;Nitrogen source carrier is heated to melt, add self-assembly gel, mix evenly;Temperature control, add 3,4-dimethyl pyrazole phosphate, and keep warm reaction;Cooling, add the blend obtained in step S200, binder, mix evenly;Extrusion granulation, after fluidized bed drying, slow-release coating is carried out, and the target compound fertilizer is obtained.The application makes disease control efficiency to improve to 89.2%, and blocks nitrosomonas AMO enzyme activity for 120 days.

The invention discloses a rhamnosyltransferase mutant and application thereof, and belongs to the technical field of genetic engineering. The catalytic activity of the rhamnosyltransferase is improved through a protein engineering modification method, so that the rhamnolipid yield of the pseudomonas aeruginosa is improved, reaches 62.87 g.L <-1 > and is 1.89 times that of a wild type, and the pseudomonas aeruginosa has a good industrial application prospect.

This application discloses a composite oil displacement system and its preparation method, belonging to the field of oilfield development technology. The composite oil displacement system provided in this application includes rhamnolipin and betaine surfactant, wherein the rhamnolipin is a biosurfactant obtained by culturing *Pseudomonas aeruginosa*. Both the rhamnolipin biosurfactant and the betaine surfactant are heat- and salt-resistant; therefore, the composite oil displacement system prepared using the rhamnolipin biosurfactant and the betaine surfactant has good compatibility with high-temperature, high-salinity reservoirs, meaning it is suitable for such reservoirs. Therefore, it can significantly improve the oil displacement effect in high-temperature, high-salinity reservoirs.

A rhamnolipid ester is useful as a cosmetic additive. A method of its production involves providing at least one rhamnolipid, reacting the at least one rhamnolipid with at least one coupling reagent, reacting the at least one rhamnolipid with a polyhydric alcohol having 1 to 32 carbon atoms, and optionally purifying the at least one rhamnolipid ester.

This invention discloses a modified rhamnolipin waterproofing agent for well workover operations in offshore oilfields and its preparation method. The method involves a one-step process where a 3-chloro-2-hydroxypropyltrimethylammonium chloride aqueous solution is added dropwise to a rhamnolipin solution, attaching a quaternary ammonium cation to the hydroxyl group of the rhamnolipin. After the reaction is complete and the solution is purified, the modified rhamnolipin waterproofing agent is obtained. The raw materials used in this invention are readily available, the preparation method is simple, and it is conducive to industrial production. The raw materials and products used have low biotoxicity and good environmental friendliness, which is beneficial for application in offshore oilfields. The solution prepared with the modified rhamnolipin waterproofing agent exhibits low surface tension, good temperature and salt resistance, and wetting improvement ability. It can significantly improve the reservoir water-locking permeability recovery rate, demonstrating not only a good waterproofing effect but also alleviating reservoir contamination problems caused by long-term production to a certain extent.

The invention relates to the technical field of foliar fertilizers, in particular to a foliar fertilizer for prolonging the leaf color viewing period of a color-leafed plant. The foliar fertilizer is prepared from the following components in 100 parts by weight: 0.5 to 1.5 parts by weight of vitamin B compounds, 0.01 to 0.05 part by weight of benzylaminopurine compounds, 0.02 to 0.05 part by weight of ethylenediamine tetraacetic acid chelated titanium, 0.3 to 0.5 part by weight of rhamnolipid, 0.4 to 0.8 part by weight of malic acid, 0.5 to 1.5 parts by weight of modified polyethylene glycol and 0.5 to 1.5 parts by weight of modified cellulose nanocrystals.

The present invention relates to an anti-odor composition. This is achieved by a composition comprising a rhamnolipid surfactant in addition to a primary anionic surfactant and a secondary amphoteric surfactant along with the inclusion of two types of organic acids.

The utility model relates to the technical field of chromatography column, concretely to a kind of chromatography column device for rhamnolipid extraction, including upper end chromatography column main part and lower end chromatography column main part, upper end chromatography column main part includes material pipe, the upside of material pipe is provided with mixing head, the side of mixing head is provided with feed pipe, the upper end of feed pipe is provided with feeding hopper, the other side of mixing head is provided with air inlet pipe, the upside of mixing head is provided with sealing plug, the downside of material pipe is provided with butt joint plug ring, lower end chromatography column main part includes filter tube.The utility model is dismantled by upper end chromatography column main part and lower end chromatography column main part split and carry out the disassembly of filter plate, it is convenient to clean operation after chromatography operation, feeding is added by feed pipe and feeding hopper, inflation pressurization is added by air inlet pipe, increase feeding passage, reduce the frequency of pull plug during chromatography, improve chromatography speed, be favorable to chromatography efficiency.

The present application relates to a kind of coal slime collector preparation method using kitchen waste oil, comprising the kitchen waste oil after being pretreated with water, emulsifier is mixed, as carbon source configuration first culture medium, Bacillus and Rhodococcus species are inoculated in it and carry out aerobic fermentation, obtain refined oil, and as carbon source configuration second culture medium, Candida species are inoculated in it and carry out aerobic fermentation, obtain mixed fatty acid, and as carbon source configuration third culture medium, Yarrowia species are inoculated in it and carry out micro-aerobic fermentation, obtain alkane mixture;Emulsified oil and / or refined oil are as carbon source configuration fourth culture medium, Pseudomonas species are inoculated in it and carry out aerobic fermentation, obtain rhamnolipid;Alkane mixture is mixed with rhamnolipid, obtain coal slime collector.The present application develops a new type of green, good coal slime collector by constructing multistage series biological transformation process.

The utility model relates to the technical field of rhamnolipid purification, in particular to a biosurfactant rhamnolipid purification device which comprises an extraction tank, a fixed pipe and a movable pipe are arranged in the extraction tank, a closed transparent cup is fixedly arranged on the outer side of the extraction tank, a discharge port controlled by a valve is formed in the lower portion of the transparent cup, and a discharge port is formed in the lower portion of the transparent cup. The top face of the transparent cup is communicated with one end of an air outlet pipe, the other end of the air outlet pipe is communicated with an air pump, the fixed pipe is sealed through a valve device, and the movable pipe is driven by a lifting mechanism to move up and down. According to the device, during primary extraction, the fixed pipe and the movable block are used for synchronous suction, so that supernate in the extraction tank can be quickly sucked out, during secondary extraction, the movable pipe is used for movement and suction, so that liquid below the supernate can be effectively prevented from being sucked in, and the accuracy of the sucked supernate is guaranteed.

The present invention relates to cosmetic cleansing preparations containing rhamnolipid and dehydroxanthan gum.

The invention discloses a preparation method of a biomass-based slow-release agent for repairing polycyclic aromatic hydrocarbon polluted underground water, which comprises the following steps: 1, preparation of soybean milk fermentation liquor: weighing soybeans, soaking the soybeans in ultrapure water according to a solid-to-liquid ratio of 1: 20-1: 50 for 12-48 hours, pulping, and adding ultrapure water until the volume is 500mL to obtain a soybean milk matrix; inoculating 10mL of polycyclic aromatic hydrocarbon polluted underground water indigenous microorganism bacterial liquid into the soybean milk matrix, and carrying out closed fermentation under a constant-temperature oscillation condition, 2, preparation of a multifunctional composite slow-release agent: weighing 35-60 parts by weight of FeSO4. 7H2O, 1-5 parts by weight of rhamnolipid, 5-10 parts by weight of humic acid, 1-5 parts by weight of methionine, 5-10 parts by weight of peanut peptide, 1-10 parts by weight of soybean lecithin, 3-10 parts by weight of a yeast extract and 40-60 parts by weight of the soybean milk fermentation broth prepared in the step 1; the method has the beneficial effects that the degradation efficiency on organic pollutants such as polycyclic aromatic hydrocarbon is remarkably enhanced, and a reliable material basis and theoretical support are provided for engineering application of an underground water bioremediation technology.

This invention discloses a rhamnosyltransferase mutant and its applications, belonging to the field of genetic engineering technology. This invention improves the catalytic activity of rhamnosyltransferase through protein engineering, thereby increasing the rhamnolipid yield of *Pseudomonas aeruginosa* to 62.87 g·L⁻¹. ‑1 It yields 1.89 times that of the wild type, and has good prospects for industrial application.

The invention belongs to the technical field of microorganisms, and particularly relates to pseudomonas aeruginosa and application thereof. According to the specific technical scheme, the pseudomonas aeruginosa HQ-2 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on October 24, 2024, and the preservation number of the pseudomonas aeruginosa HQ-2 is GDMCC NO.65338. The pseudomonas aeruginosa HQ-2 is named as Pseudomonas aeruginosa HQ-2. The pseudomonas aeruginosa HQ-2 is named as Pseudomonas aeruginosa HQ-2. The pseudomonas aeruginosa HQ-2 is low in toxicity and sensitive to four indicating antibiotics, the yield of rhamnolipid is not reduced, and the pseudomonas aeruginosa HQ-2 is wide in application prospect.

The present invention relates to cleaning compositions for reducing off-flavors on surfaces, in particular to compositions for cleaning soft surfaces, such as those of fabrics. The composition ensures that the fabric remains free of off-odor for a long period of time after the surface is cleaned with the composition. This is accomplished by the combination of rhamnolipids and bacillus bacterial spores in a composition containing a surfactant.

The invention relates to a microbial agent and a preparation method thereof, and belongs to the technical field of microbial agent preparation, and the preparation method specifically comprises the following steps: S1, preparing a pseudomonas aeruginosa fermentation broth, adding a flocculant and biochar, carrying out shaking table oscillation treatment, filtering, collecting a filtered product, and drying to obtain rhamnolipid modified biochar; s2, carrying out multiplication culture on the composite strain, and after the multiplication culture is finished, collecting to obtain a multiplication strain solution; mixing the proliferation bacteria solution with a phosphate buffer solution to obtain a compound bacteria solution; and mixing the rhamnolipid modified biochar with a composite bacterial liquid, and drying to obtain the rhamnolipid-containing microbial agent. According to the technical scheme, an innovative process of rhamnolipid-biochar synergistic loading and composite strain function strengthening is provided, and the prepared microbial inoculum has the effects of improving soil and promoting fertilizer.

This invention discloses a self-thickening, moisturizing, and oil-controlling gentle facial cleanser and its preparation method, belonging to the field of cosmetic technology. The raw materials are composed of the following percentages by weight: rhamnolipin 2%~8%, alkyl glycoside 5%~15%, cationic polymer 0.1%~1.5%, moisturizer 2%~6%, plant extract 0.5%~3%, pH adjuster (the amount needed to adjust the system pH to 5.0~7.0), preservative 0.1%~1.0%, and water as the balance. Through the ternary system of rhamnolipin-alkyl glycoside-cationic polymer, self-thickening and foam stability are achieved through electrostatic interaction and steric hindrance synergy, without relying on traditional thickeners such as carbomer. Simultaneously, it possesses excellent moisturizing and oil-controlling properties, with an oil removal rate ≥89%, a skin moisture content increase of ≥15% after 4 hours of use, and an oil secretion inhibition rate of ≥20% after 4 hours, with extremely low irritation. This invention solves the technical problem of low viscosity and poor foam performance in the rhamnolipin and alkyl glycoside compound system.

A sulfate-free personal care composition that includes a rhamnolipid surfactant and provides good cleaning and foaming properties. The rhamnolipid surfactant has a single rhamnose head group (i.e., mono-rhamno) and a single lipid tail group (i.e., mono lipid). The single lipid tail group has a carbon chain length of 10 to 13. The composition may include one or more additional surfactants selected from anionic surfactants, amphoteric surfactants, zwitterionic surfactants, non-ionic surfactants, cationic surfactants and combinations thereof, as long as the additional surfactant is free of sulfates. The composition also includes a dermatologically acceptable carrier.

The application discloses a surfactant composition, a surfactant system and application thereof, and belongs to the technical field of cosmetics, and comprises the following components in parts by weight: 5-11 parts of sodium C14-16 olefin sulfonate, 1-6 parts of an amino acid surfactant, 0.5-5 parts of an alkyl polyglycoside surfactant, 0.1-2 parts of a bio-based surfactant and 1-5 parts of an amphoteric surfactant; the bio-based surfactant comprises at least one of sophorolipid, rhamnolipid, soapberry extract and oil tea dregs extract. The composition disclosed by the application has very low surface tension and cmc value, the molecules in a surface adsorption layer are closely arranged to promote surface adsorption, the foaming performance and foaming stability are effectively improved, good stability is achieved in a wide pH (5-10) range, excellent high and low temperature stability is achieved, the surfactant composition disclosed by the application has low irritation, is safe to use, and has a wide application prospect.

To increase occupational safety and provide improved homogeneous colour appearance of a dry coating film in the production of a coating composition.SOLUTION: The present invention discloses the use of rhamnolipids and / or sophorolipids in coating compositions, for improved speed of wetting of pulverulent formulation constituents in the coating compositions, which leads to a reduction in dust nuisance during incorporation of the pulverulent formulation constituents, resulting in increased occupational safety in the production of the coating compositions, providing the improved homogeneous colour appearance of a dry coating film with regard to difference in hue, ascertained from the ΔE and visual assessment.SELECTED DRAWING: Figure 1

The invention relates to application of rhamnolipid in preparation of a functional product for preventing and controlling outbreak of aurelia. The invention particularly relates to the application of the rhamnolipid in preparation of an aurelia aurita killing agent. The aurelia comprises at least one of aurelia scyphistoma, discoid and jellyfish. The rhamnolipid has high killing activity on aurelia scyphistoma, ephyra and jellyfish, the aurelia scyphistoma and ephyra can die completely when the concentration of the rhamnolipid is larger than 12 mg / L and the rhamnolipid is exposed for 48 h, and the aurelia jellyfish can die completely when the concentration of the rhamnolipid is larger than 18 mg / L and the rhamnolipid is exposed for 48 h. Meanwhile, compared with a botanical pesticide, the rhamnolipid has higher safety to other aquatic organisms.

The application belongs to the field of admixtures, and particularly relates to a concrete thickening water-retaining agent and a preparation method and application thereof. The concrete thickening water-retaining agent is obtained by free radical polymerization reaction of an unsaturated acid monomer I, an unsaturated amide monomer, an unsaturated sulfonate monomer and a functional monomer; and the functional monomer is an esterification reaction product of rhamnolipid and an unsaturated acid monomer II. The key of the application is to introduce the esterification reaction product of rhamnolipid and the unsaturated acid monomer II as a copolymerization monomer into a copolymerization reaction system of the unsaturated acid monomer I, the unsaturated amide monomer and the unsaturated sulfonate monomer, so that the molecular structure of the concrete thickening water-retaining agent can be changed, the obtained concrete thickening water-retaining agent not only has excellent thickening and water-retaining properties, but also can improve the stability thereof in concrete.

The invention discloses a rhamnosyltransferase mutant and application thereof, and belongs to the technical field of genetic engineering. The catalytic activity of the rhamnosyltransferase is improved through a protein engineering modification method, so that the rhamnolipid yield of the pseudomonas aeruginosa is improved, reaches 62.87 g.L <-1 > and is 1.89 times that of a wild type, and the pseudomonas aeruginosa has a good industrial application prospect.