307 results about "Rhamnolipid" patented technology

The invention provides a high-temperature-resistant and high-salt-resistant nano imbibition agent as well as a preparation method and application thereof. The high-temperature-resistant and high-salt-resistant nano imbibition agent is prepared from amination modified mesoporous silica nanoparticles, nano cellulose, rhamnolipid, an anionic surfactant, citrate modified sodium zirconium phosphate, polyethylene glycol-b-polycaprolactone and a sulfydryl-carboxyl imidazoline derivative, therefore, the composite carrier formed by the aminated mesoporous silica nanoparticles and the nanocellulose has high mechanical strength and uniform dispersity, the oil-water interfacial tension is reduced through the synergistic effect of the rhamnolipid and the anionic surfactant, and the crude oil stripping efficiency is remarkably improved; the citrate modified sodium zirconium phosphate enables the salt resistance of the system to be greatly improved, and in a high-temperature and high-salt oil reservoir environment, the nano imbibition agent can remarkably improve the crude oil recovery rate and reduce the injection pressure and dosage; meanwhile, good adsorption resistance is achieved, and reservoir blockage is prevented; in addition, low-toxicity corrosion inhibition components protect equipment, and the service life of an oil well is prolonged.

The invention discloses a seed pelleting formula for improving salt tolerance of suaeda salsa in coastal saline-alkali soil and a processing method, the pelleting formula comprises 348-492 parts of a filler, 348-492 parts of a binder, 348-492 parts of a water-retaining agent and 348-492 parts of a biological stimulant, and the filler comprises attapulgite, talcum powder and vermiculite; the binder is 73-89 parts and comprises sodium carboxymethyl cellulose, polyvinyl alcohol and starch; the water-retaining agent accounts for 30 to 42 parts, and the water-retaining agent is polyacrylamide; the biological stimulant is prepared from the following components in parts by weight: 20-78 parts of yeast metabolite, potassium fulvate, seaweed essence, rhamnolipid and a microbial agent; the processing method comprises the steps of seed selection, raw material preparation, layered coating, low-temperature drying, selection and sieving, and polishing and shaping. According to the invention, a layered coating process is adopted to break through a traditional single mixed coating mode, an isolation breathable microenvironment is firstly constructed by the filling layer, then growth-promoting stress-resistant nutrient substances are injected by the active layer, and finally, the shaping layer is used for locking and shaping and strengthening protection, so that the seeding performance, salt tolerance, germination activity and seedling stress resistance of the pellets are remarkably improved.

The invention relates to microbial colorant compositions and associated processes to produce the same. Microbial colorant compositions are produced by combining microbially derived pigment molecules and microbial biosurfactants. The pigment molecules are obtained either directly from a fermentation broth or by extracting a precursor and transforming it into a pigment using chemical or enzymatic processes. A separately produced biosurfactant, such as a rhamnolipid or surfactin, is added to the obtained pigment molecule to create a pre-dispersed formulation. The biosurfactant may be added at various steps of This approach improves pigment dispersion, solubility, and substrate compatibility, enabling stable aqueous formulations without synthetic additives. The resulting compositions are suitable for wide range of applications including in textiles, food, cosmetics, and polymer materials. Processes for producing and combining the pigment and biosurfactant are also disclosed, including variations in fermentation, extraction, and formulation stages.

The invention discloses a Pseudomonas putida high-yield rhamnolipid fermentation medium based on a step-by-step optimization strategy, an optimization method and application thereof, through the step-by-step strategy of component screening-single-factor optimization-multi-factor interactive optimization, a mixed carbon source and a mixed nitrogen source are taken as cores, and a Plackett-Burman test, a steepest climbing test and Box-Behnken response surface design are combined, so that the high-yield rhamnolipid fermentation medium for the pseudomonas putida high-yield rhamnolipid is obtained. Optimizing to obtain a special fermentation culture medium; two genetic engineering strains estA-2 and estA-3 are constructed by heterologous expression of key genes, and the supply and extracellular transport efficiency of a rhamnolipid synthesis precursor is enhanced; the recombinant strain is applied to the optimized culture medium, and efficient production of a shake flask and a 5L fermentation tank is achieved. The rhamnolipid yield of the estA-3 strain in a 5L fermentation tank reaches 35.59 + / -2.18 g / L, the conversion rate is 0.282 g / g, the productivity is 0.404 g / L / h, the rhamnolipid yield, the conversion rate and the productivity are obviously superior to those in the prior art, and a key technical support is provided for industrial production of rhamnolipid.

Compositions having at least one biosurfactant and at least one persicomycin. The at least one biosurfactant is selected from the group consisting of rhamnolipids, glucolipids and sophorolipids. The compositions optionally have at least one enzyme, at least one non-biosurfactant and / or at least one selected from the group consisting of an anti-redeposition polymer and a soil release polymer. The compositions clean surfaces of an article in a process for cleaning said article.

The present invention discloses a biochar-supported rhamnolipid-coated nano-zero valent iron modified material, its preparation method and application. The modified nano-zero valent iron material includes biomass carbon, rhamnolipid and nano-zero valent iron. The rhamnolipid is modified on the surface of the nano-zero valent iron and loaded on the biomass carbon. The mass ratio of nano-zero valent iron, rhamnolipid and biochar is 1:0.05-0.2:0.5-2. The preparation method is to mix the rhamnolipid solution with the ferrous sulfate solution, then mix with the biomass carbon, and dropwise add the NaHB4 solution for reaction to obtain the modified nano-zero valent iron material. The biochar-supported rhamnolipid-coated nano-zero valent iron material of the present invention has the advantages of good stability, high reaction activity, strong dispersion performance, etc. It has good removal efficiency for the chlorinated flame retardant dechlorane in the environment and will not cause secondary pollution, and has potential good application value and broad application prospects in the field of organic polluted soil and water treatment.

The invention relates to the technical field of cleaning agents, and particularly discloses a composite cleaning agent as well as a preparation method and application thereof. The composite cleaning agent comprises the following raw material components in percentage by mass: 5%-10% of inorganic particles, 5%-15% of a surfactant, 15%-30% of a cleaning aid and the balance of water, wherein the surface active agent is prepared from rhamnolipid, fatty acid methyl ester ethoxylate, cocamidopropyl betaine and sophorolipid; the inorganic particles comprise soluble sodium salt and nano silicon dioxide; the cleaning auxiliary agent comprises a geranium extracting solution, a herba portulacae extracting solution, lauric acid and glycerol. The composite cleaning agent is simple in raw material components, simple and convenient in preparation method, low in cost and free of secondary pollution to the environment. The composite cleaning agent effectively solves the problems that an existing train cleaning agent is poor in cleaning effect, complex in manufacturing process and unfriendly to the environment.

The invention relates to pseudomonas putida engineering bacteria for producing rhamnolipid as well as a construction method and application of the pseudomonas putida engineering bacteria, the engineering bacteria take pseudomonas putida KT2440 with phaC1, phaC2, algD and fliC gene deletion as a chassis cell, and rhlAB, rmlACBD, algC and FabY genes are over-expressed in the chassis cell. Pseudomonas putida KT2440 with biological safety is used as a chassis cell for gene modification, the constructed engineering bacteria are used for fermentation production of rhamnolipid, on the basis of rhamnolipid yield increase, good physiological adaptability and fermentation stability are shown, and the rhamnolipid yield is increased. And the modified engineering strain can stably and efficiently produce rhamnolipid with a specific structure.

The invention belongs to the field of bioengineering, and particularly relates to a flexible mycelium composite material as well as a preparation process and application thereof. The flexible mycelium composite material disclosed by the invention is prepared by inoculating an edible fungus seed solution into a lignocellulose matrix, mixing, filling a mold and integrally fermenting, and the preparation process is simple. According to the method, rhamnolipid is added into an edible fungus seed solution culture medium, so that the enzyme activity of cellulase in the seed solution is effectively improved, the degradation and utilization of cellulose and hemicellulose in a lignocellulose matrix are further improved, the planting capacity of mycelia on the surface of lignocellulose is improved, the biomass of the mycelia is improved, and the yield of the mycelia is increased. Finally, the toughness of the flexible mycelium composite material is improved, so that the bending property of the flexible mycelium composite material is improved. Meanwhile, the flexible mycelium composite material prepared by the preparation method disclosed by the invention is biologically converted by utilizing agricultural and forestry wastes, the material utilization rate can reach 100%, and the flexible mycelium composite material is free from environmental pollution and has a wide industrial application prospect.

The invention relates to the technical field of crop cultivation. In particular to a rice leaf fertilizer and a fertilizing method and application thereof. The rice leaf fertilizer is prepared from the following components in percentage by weight: 10 to 20 percent of chitin derivative, 5 to 15 percent of compound amino acid, 5 to 10 percent of composted livestock and poultry manure, 5 to 15 percent of fulvic acid, 1 to 3 percent of zinc sulfate, 0.5 to 2 percent of borax, 0.2 to 0.5 percent of potassium silicate, 0.1 to 0.5 percent of polyether modified siloxane, 0.05 to 0.1 percent of rhamnolipid, 0.1 to 0.15 percent of betaine, 1 to 2 percent of sodium alginate and the balance of water. By applying the foliar fertilizer in drought and salt stress environments, the content of beta-glucan in rice is increased, the rice foliar fertilizer disclosed by the invention not only can be popularized for planting rice in northern drought regions in China, but also the planted rice has higher beta-glucan content, and has a health assisting effect on diabetics with limited starch consumption.

The invention provides a method for promoting co-fermentation of seaweed sludge to produce a carbon source by utilizing microbial pretreatment. The method comprises the following steps: adding a microbial agent with protein degradation capability into the sludge; adding free nitrous acid and rhamnolipid into the sludge obtained in the step 1, and performing pretreatment in an anaerobic environment; mixing the pretreated sludge with seaweed, and carrying out seaweed and sludge co-fermentation to obtain supernate of a fermentation mixture; the method for promoting co-fermentation of the seaweed sludge by utilizing microbial pretreatment can be widely applied to treatment and recycling of the sludge, the treatment efficiency of the sludge can be improved, and the treatment cost of the sludge can be reduced. The prepared seaweed sludge fermentation liquor can be used as an external carbon source for sewage treatment, can provide sufficient nutrient substances for microorganisms, and effectively promotes denitrification and phosphorus-accumulating bacteria metabolism in the sewage treatment process, so that the sewage nitrogen and phosphorus removal effect is improved.

The invention provides a medium-low permeability oil reservoir rhamnolipid foam oil displacement system and a screening method and application, and relates to the technical field of microbial oil recovery, the medium-low permeability oil reservoir rhamnolipid foam oil displacement system comprises the following steps: S1, screening an applied oil reservoir; s2, determining a rhamnolipid foam flooding system based on the oil reservoir mineralization degree; s3, based on the porosity of the oil reservoir, the injection mode of a rhamnolipid foam oil displacement system is determined, the rhamnolipid foam oil displacement system comprises a foaming agent, a foam stabilizer, nitrogen and water, the foaming agent comprises one or more of dirhamnose difat, dirhamnose monofat, single rhamnose monofat and single rhamnose difat fermentation liquor, and the foam stabilizer comprises one or more of dirhamnose difat, dirhamnose monofat, single rhamnose monofat and single rhamnose difat fermentation liquor. The foam stabilizer is prepared from one or more of xanthan gum, welan gum, Wollan gum, Dide gum, gellan gum, rhamnose gum, diidine gum, plant cellulose and bacterial cellulose. The method has the beneficial effects that the oil reservoir application range is wide, the pertinence is high, the field test effect is good, efficient generation and long-acting migration of foam in the oil reservoir are guaranteed, green and high-quality development of old oil fields is promoted, and achievement of the national dual-carbon strategy is assisted.

The invention discloses a rhamnose continuous production system and a rhamnose continuous production method. A rhamnolipid continuous fermentation system and a rhamnolipid continuous hydrolysis system are coupled. The system comprises a fermentation tank, an overflow tank, a ceramic membrane, a rhamnolipid continuous hydrolysis reactor and the like. By adopting the method to produce rhamnose, the problem that the fermentation temperature of rhamnolipid is inconsistent with the hydrolysis temperature of rhamnolipid is solved, the hydrolysis reaction efficiency of rhamnolipid is improved, meanwhile, the influence of rhamnolipid bubbles on a fermentation system is also solved, the production efficiency of rhamnose is improved, the production cost of rhamnose is reduced, and good economic benefits are achieved.

The application discloses a kind of compound fertilizer with nitrogen fertilizer slow-release and biocontrol function and preparation method, it is related to microbial compound fertilizer field, including the following components: nitrogen source carrier, physical net trapping material, 3,4-dimethyl pyrazole phosphate, biocontrol component, binder.A kind of preparation method, including the following steps: after silanization modification treatment is carried out to attapulgite, with polyglutamic acid aqueous solution mixed reaction, obtain self-assembly gel;Bacillus subtilis is embedded and handled, rhamnolipid is pre-emulsified, chitosan oligosaccharide is crosslinked with boric acid treatment, and is blended;Nitrogen source carrier is heated to melt, add self-assembly gel, mix evenly;Temperature control, add 3,4-dimethyl pyrazole phosphate, and keep warm reaction;Cooling, add the blend obtained in step S200, binder, mix evenly;Extrusion granulation, after fluidized bed drying, slow-release coating is carried out, and the target compound fertilizer is obtained.The application makes disease control efficiency to improve to 89.2%, and blocks nitrosomonas AMO enzyme activity for 120 days.

The invention discloses a rhamnosyltransferase mutant and application thereof, and belongs to the technical field of genetic engineering. The catalytic activity of the rhamnosyltransferase is improved through a protein engineering modification method, so that the rhamnolipid yield of the pseudomonas aeruginosa is improved, reaches 62.87 g.L <-1 > and is 1.89 times that of a wild type, and the pseudomonas aeruginosa has a good industrial application prospect.

This application discloses a composite oil displacement system and its preparation method, belonging to the field of oilfield development technology. The composite oil displacement system provided in this application includes rhamnolipin and betaine surfactant, wherein the rhamnolipin is a biosurfactant obtained by culturing *Pseudomonas aeruginosa*. Both the rhamnolipin biosurfactant and the betaine surfactant are heat- and salt-resistant; therefore, the composite oil displacement system prepared using the rhamnolipin biosurfactant and the betaine surfactant has good compatibility with high-temperature, high-salinity reservoirs, meaning it is suitable for such reservoirs. Therefore, it can significantly improve the oil displacement effect in high-temperature, high-salinity reservoirs.

The invention relates to the technical field of toothpaste preparation, and particularly discloses a Chinese herbal medicine toothpaste formula and a preparation method thereof, the Chinese herbal medicine toothpaste formula comprises the following raw materials by weight: 17-18 parts of a functional component, 2.4-2.8 parts of a slow release synergist, 20-22 parts of a friction agent, 28-32 parts of a humectant, 2.6-3.4 parts of a surfactant, 0.16-0.18 part of a preservative, 1.8-2 parts of a thickener, 1.4-1.6 parts of essence, 5.8-6.2 parts of a flavoring agent and 16-18 parts of deionized water; taxillus sutchuenensis, herba cepbalanoplosis segeti, plantain herb, herba pyrolae and fructus forsythiae are used as core materials of functional components, gelatin and modified sodium alginate are used as wall materials, the functional components are prepared, and a slow-release synergist containing anthocyanin, bromelain and rhamnolipid is added, so that the effects of removing stains and whitening skin are achieved; moreover, release of quercetin, quercitrin and other components in the traditional Chinese medicine extract is promoted, and the anti-inflammatory, bleeding-stopping, halitosis-removing, stain-removing and whitening effects of the toothpaste are improved.

A rhamnolipid ester is useful as a cosmetic additive. A method of its production involves providing at least one rhamnolipid, reacting the at least one rhamnolipid with at least one coupling reagent, reacting the at least one rhamnolipid with a polyhydric alcohol having 1 to 32 carbon atoms, and optionally purifying the at least one rhamnolipid ester.

This invention discloses a modified rhamnolipin waterproofing agent for well workover operations in offshore oilfields and its preparation method. The method involves a one-step process where a 3-chloro-2-hydroxypropyltrimethylammonium chloride aqueous solution is added dropwise to a rhamnolipin solution, attaching a quaternary ammonium cation to the hydroxyl group of the rhamnolipin. After the reaction is complete and the solution is purified, the modified rhamnolipin waterproofing agent is obtained. The raw materials used in this invention are readily available, the preparation method is simple, and it is conducive to industrial production. The raw materials and products used have low biotoxicity and good environmental friendliness, which is beneficial for application in offshore oilfields. The solution prepared with the modified rhamnolipin waterproofing agent exhibits low surface tension, good temperature and salt resistance, and wetting improvement ability. It can significantly improve the reservoir water-locking permeability recovery rate, demonstrating not only a good waterproofing effect but also alleviating reservoir contamination problems caused by long-term production to a certain extent.

The present invention relates to the use of rhamnolipids and / or sophorolipids in coating compositions for improving the wetting rate of powdery formulation components in such compositions, which results in a reduction of dust nuisance during the addition of the powdery formulation components and thus in an increase in occupational safety during the production of the coating compositions, and in obtaining an improved uniform color appearance of the dry paint film with respect to color tone differences, which are determined by ΔE and visual evaluation.

The invention provides a method for separating endotoxin from rhamnolipid fermentation liquor, which comprises the following steps: 1) removing thalli containing the endotoxin from the rhamnolipid fermentation liquor through centrifugal separation to obtain clear liquor; (2) adding a surfactant into the clear liquid obtained in the step (1), adding calcium oxide at the same time, adjusting the pH value to be more than 10, separating endotoxin-containing cell debris through flocculation, and then centrifuging to obtain clear liquid; (3) adding acid into the clear liquid obtained in the step (2) to adjust the pH value to 2-3, and then performing centrifugal separation to obtain rhamnolipid precipitate; (4) adding alcohol into the rhamnolipid precipitate obtained in the step (3), and dissolving to form a solution; (5) adding an organic solvent into the solution obtained in the step (4), and extracting to obtain an extracting solution; and 6) removing the alcohol and the organic solvent from the extract obtained in the step 5), and then adjusting the pH value to 6-7 to obtain the rhamnolipid aqueous solution. The method is high in biological safety and easy for large-scale production. The endotoxin content of the product is lt; 0.1 EU / mg, which meets the medical standard.

According to the environment-friendly cleaning agent additive and the preparation method thereof, an environment-friendly water-based cleaning agent is prepared from waste peel resources such as orange peel and pericarpium citri reticulatae by means of microbial fermentation of the waste peel resources, the waste peel resources are fully utilized, effective components are extracted through a green and environment-friendly process, the production cost is reduced, and the environment-friendly cleaning agent additive is suitable for industrial production. And the environmental pollution is also reduced. The combined use of limonene, rhamnolipid, bio-enzyme and alkaline secretions significantly enhances the decontamination effect, fully utilizes natural resources, and reduces the dependence on chemical synthesis raw materials. The prepared cleaning agent additive has efficient degreasing capacity, is environment-friendly, and meets the requirements of sustainable development.

The invention relates to the technical field of foliar fertilizers, in particular to a foliar fertilizer for prolonging the leaf color viewing period of a color-leafed plant. The foliar fertilizer is prepared from the following components in 100 parts by weight: 0.5 to 1.5 parts by weight of vitamin B compounds, 0.01 to 0.05 part by weight of benzylaminopurine compounds, 0.02 to 0.05 part by weight of ethylenediamine tetraacetic acid chelated titanium, 0.3 to 0.5 part by weight of rhamnolipid, 0.4 to 0.8 part by weight of malic acid, 0.5 to 1.5 parts by weight of modified polyethylene glycol and 0.5 to 1.5 parts by weight of modified cellulose nanocrystals.

The invention relates to the technical field of colored ribbon spraying agents, in particular to a low-temperature-resistant environment-friendly colored ribbon spraying agent and a preparation method thereof. The invention relates to a low-temperature-resistant environment-friendly colored ribbon spray, which is prepared from 20 to 30 parts of deionized water, 0.2 to 0.4 part of preservative, 0 to 0.3 part of water-soluble pigment, 0.1 to 0.2 part of coconut oil diethanolamide, 0.1 to 0.2 part of perfluorooctyl quaternary ammonium iodide, 0.1 to 0.2 part of rhamnolipid, 0.1 to 0.2 part of sodium vinylsulfonate, 4 to 6 parts of ethanol, 15 to 25 parts of filling powder, 5 to 8 parts of acrylic resin, 5 to 7 parts of ethylene glycol monobutyl ether and 20 to 30 parts of propellant. The colored ribbon spraying agent successfully solves the problem that an existing colored ribbon spraying agent is instable in spraying under the low-temperature condition, and the excellent spraying effect is still kept in the cold environment.

The present invention relates to an anti-odor composition. This is achieved by a composition comprising a rhamnolipid surfactant in addition to a primary anionic surfactant and a secondary amphoteric surfactant along with the inclusion of two types of organic acids.

Rhamnolipids and / or sophorolipids can be used in coating compositions for improvement of the speed of wetting of pulverulent formulation constituents in such compositions, which leads to a reduction in dust nuisance during incorporation thereof and to the increase in occupational safety in the production of coating compositions. Rhamnolipids and / or sophorolipids can also be used in coating compositions for obtaining improved homogeneous colour appearance of the dry paint film with regard to difference in hue, ascertained from the delta E and visual assessment.

The utility model relates to the technical field of chromatography column, concretely to a kind of chromatography column device for rhamnolipid extraction, including upper end chromatography column main part and lower end chromatography column main part, upper end chromatography column main part includes material pipe, the upside of material pipe is provided with mixing head, the side of mixing head is provided with feed pipe, the upper end of feed pipe is provided with feeding hopper, the other side of mixing head is provided with air inlet pipe, the upside of mixing head is provided with sealing plug, the downside of material pipe is provided with butt joint plug ring, lower end chromatography column main part includes filter tube.The utility model is dismantled by upper end chromatography column main part and lower end chromatography column main part split and carry out the disassembly of filter plate, it is convenient to clean operation after chromatography operation, feeding is added by feed pipe and feeding hopper, inflation pressurization is added by air inlet pipe, increase feeding passage, reduce the frequency of pull plug during chromatography, improve chromatography speed, be favorable to chromatography efficiency.

The invention discloses Pseudomonas aeruginosa NJUXR-CC-3 and application of the Pseudomonas aeruginosa NJUXR-CC-3. The Pseudomonas aeruginosa NJUXR-CC-3 is obtained by taking Pseudomonas aeruginosa ATCC 9027 as a starting strain and performing ARTP (Attenuated Region Transfer Protocol) mutagenesis, and the Pseudomonas aeruginosa NJUXR-CC-3 is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M2025218. The pseudomonas aeruginosa NJUXR-CC-3 can be used for efficiently synthesizing an ampholytic surfactant rhamnolipid by taking vegetable oil as a carbon source; the paraffin inhibition rate of the paraffin inhibitor based on the Pseudomonas aeruginosa is 79.88%, which is 81.59% higher than that of the original strain Pseudomonas aeruginosa ATCC 9027, and the Pseudomonas aeruginosa can be used for preventing pipeline blockage during high-wax crude oil transportation or wax deposition during storage, and has high industrial application value.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a fermentation process for efficiently preparing rhamnolipid. The fermentation process comprises the following steps: inoculating pseudomonas aeruginosa into an enrichment culture medium, activating and culturing for 18-24 hours to obtain a seed bacteria solution, inoculating the seed bacteria solution into a sterilized fermentation culture medium, fermenting for 90-180 hours in an aerobic environment at 25-37 DEG C, and regulating the pH in the fermentation process to obtain the pseudomonas aeruginosa strain. At the same time, adding a fermentation accelerator within 20-40th h after fermentation, and starting to supplement a carbon source and a nitrogen source at 40th h to adjust the carbon-nitrogen ratio in the fermentation medium to obtain a fermentation broth; and separating and purifying the obtained fermentation liquor to obtain the rhamnolipid. According to the method, the specific fermentation strain, the fermentation culture medium and parameter conditions in the fermentation process are selected, and the defoaming agent and the fermentation accelerant are added in the fermentation process, so that the pseudomonas aeruginosa can secrete high-yield rhamnolipid, and the production cost of the rhamnolipid is reduced to a certain extent.