459 results about "Rhamnolipid" patented technology

The invention discloses a preparation method and application of rhamnolipid. In the method, the rhamnolipid is produced by using waste grease as carbon source, and utilizing the fermentation of pseudomonas aeruginosa; and with the combination of the defoaming effect of ethanol, the yield of the rhamnolipid can reach 35 to 50 g/L and the cost is about 25 to 40 percent lower than that of the conventional method. When the solution containing 20 to 500 mg/L of rhamnolipid is used for processing plant disease fungus, the ratio of inhibiting disease fungal mycelium growth and spore germination can reach 40 to 90 percent and the plant fungal diseases can be effectively inhibited; the solution containing 20 to 500 mg/L of rhamnolipid has an obvious effect of killing cockroaches and aphids; and in addition, when the rhamnolipid is used as an aid of pesticide, fertilizer and feed and a dehydrating agent of oil-contained sludge by replacing a chemical surface active agent, the effect is obvious.

The invention relates to a mixture composition comprising rhamnolipids, to a process for its preparation, to its use for producing formulations and to formulations comprising this mixture composition.

The invention relates to cells and nucleic acids and also use thereof for producing rhamnolipids, and also methods for producing rhamnolipids.

The invention relates to a combined restoring method of polycyclic aromatic hydrocarbon contaminated soil, comprising the following restoring steps of: planting lucerne as perennial leguminous plants in the polycyclic aromatic hydrocarbon contaminated soil; inoculating a mixed bacterial inoculum comprising arbuscular mycorrhizal fungi, bacillus and flavobacterium; and meanwhile, adding rhamnolipid as a biosurfactant. By rhizosphere effect generated by plant root exudates, the number and the metabolic activity of soil microorganisms are increased; and by inoculating the arbuscular mycorrhizal fungi, the plant growth is promoted, and the root exudates are increased, thereby increasing the root accumulation of organic pollutants and the number of root soil microorganisms. The inoculated high-efficiency polycyclic aromatic hydrocarbon degrading bacteria use plant root secretions as a carbon source and an energy source and can quickly grow and propagate. The polycyclic aromatic hydrocarbon is stripped from soil grains by the biosurfactant, and the bioavailability and the degradability of the organic pollutants are increased.

The present invention relates to rhamnolipid-based formulations to clean, disinfect, deodorize, and act as an antimicrobial and antifungal agent for living and working environments. In addition, the present invention relates to the use of rhamnolipids to create a bio-film when applied to a surface, which prevents the growth of bacteria and fungus. This technique is especially useful to create clean surface areas for medical procedures, chemical testing, during food preparation, as well as for daycare centers and hospitals.

The invention relates to a water block damage treating agent based on a biological surface active agent and a preparation method thereof, and belongs to the technical field of oil field working fluid additives. The water block damage treating agent comprises the following components in percentage by weight: 30%-70% of microbe fermentation liquor, 1%-5% of non-ionic surface active agents, 0.5%-1% of fluorocarbon surface active agents, 5%-8% of cationic surface active agents and the balance of distilled water, wherein the microbe fermentation liquor is the microbe strain fermentation liquor of the biological surface active agent which generates one of rhamnolipid, lipopeptid, trehalose glycolipid and sophorolipid. The water block damage treating agent disclosed by the invention has the advantages of relatively broad-spectrum effect, high-efficiency water block release and easiness for large-area popularization.

The invention relates to a bacterium S2 for efficiently generating biosurfactant and a fermentation culture medium thereof, belonging to the field of biotechnology. The bacterium S2 is gram-negative bacterium which is rod-shaped, is (6-8)*(8-12) micrometers in size and has no spores and capsules. The bacterial strain belongs to the pseudomonas aeruginosa through identifying by morphological and physio-biochemical characteristics and molecular biology, and the collection code is CGMCC No.3034. The best fermentation culture medium is a novel culture medium which takes 50g/L of rapeseed oil as the only carbon source. The rhamnolipid is the only fermentation product of the bacterial strain and has high yield. The CMC (Critical Micelle Concentration) value of the fermentation liquid is 0.25 g/L; compared with the common chemical surfactant, the bacterium has more obvious emulsification and durability on hydrophobic substances, such as oils and the like.

The invention relates to a young pigeon feed, which is obtained by mixing the following raw materials by weight: 20-45% of broiler feed material, 5-20% of corn, 5-10% of pea, 5-10% of bean cake, 1-3% of hemp seed, 6-12% of red soil, 2-4% of bentonite, 8-15% of yellow sand, 2-4% of salt, 2-8% of milk powder, 5-20% of bean meal, 1-3% of bone meal, 2-6% of edible oil, 1-2% of baking powder, 2-5% of phagostimulant, 2-6% of composite trace element, 1-5% of composite vitamin and 1-3% of additive, wherein the additive contains one or two of mannosylerythritol lipid or rhamnolipid. By addition of nutrient components of the young pigeon feed such as the additive, phagostimulant and the like, the feed disclosed by the invention is improved in taste, has a comprehensive nutrition, stimulates appetite of young pigeons and is beneficial to the growth of young pigeons.

The invention discloses a rhamnolipid high-yielding bacterial strain, and applications thereof. The rhamnolipid high-yielding bacterial strain is named as pseudomonas aeruginosa KT1115, is preserved at China Center for Type Culture Collection, and the preservation number is CCTCC M 2016686. The rhamnolipid high-yielding bacterial strain is stable in hereditary features and high in rhamnolipid yield, and is obtained via ARTP induced mutation, blue gel plate screening, and secondary screening. According to fermentation broth of pseudomonas aeruginosa KT1115, liquid surface tension is reduced to 24.8mN/m<2>, and emulsification value is as high as 80%. Optimization of fermentation conditions including carbon source and nitrogen source single factors, cheap crude glycerine and swill-cooked dirty oil are taken as a composite carbon source, so that production cost is reduced greatly. The rhamnolipid yield of pseudomonas aeruginosa KT1115 is 50.7g/L, is increased by 18.1 times compared with that of a starting strain, conversion rate is 0.75g/g, and is relatively high. Application prospect of pseudomonas aeruginosa KT1115 in further industrialized application is promising.

The invention provides a method for cleaning aged oil sludge. The method comprises the steps of firstly collecting aged oil sludge from an oil field, compounding a biological surfactant (rhamnolipid), a chemical surfactant (TX-100) and an aid (NaSiO39H2O) in a certain mass ratio under conditions of certain temperature, stirring speed, stirring time, liquid-solid ratio and mass concentration, cleaning the aged oil sludge, standing, and separating after an oil phase, a liquid phase and a solid phase are layered. Due to large proportion of petroleum heavy components in the aged oil sludge, the aged oil sludge is difficult to clean. According to the method, the biological surfactant, the chemical surfactant and the aid are compounded to clean the aged oil sludge, so that a relatively good elution effect is achieved, and the biological surfactant, the chemical surfactant and the aid have a synergistic effect during the compounding and cleaning.

The invention relates to a composite printing and dyeing auxiliary as well as a preparation method and an application of the auxiliary. The composite printing and dyeing auxiliary is prepared by compounding polyethylene glycol diamine, N-methylol acrylamide, sodium dodecyl benzene sulfonate, ammonium persulfate, di-rhamnolipid, a plant extract, tannic acid, sodium taurate, 1-carboxyl-N,N,N-trimethyl polycrylactone, tetradecyl dimethyl ammonium chilride, 1-phenoxy-2-propyl alcohol, 2-p-toluene sulfonic acid phenylamino-4-chloro-6-beta-naphthoxy triazine, silicon dioxide, europium chloride, ethanol and polyethylene glycol 600. As specific polymer components, the plant extract, quaternary ammonium salt, natural amino acid and natural esters are introduced, a rare earth complex is formed and other suitable components are added under compounding and synergistic effects, the composite printing and dyeing auxiliary has excellent technical effects of burnishing, protecting the color and improving the color fastness, and has the certain antibacterial and antistatic functions, and therefore, the composite printing and dyeing auxiliary has a wide industrial application prospect in the textile printing and dyeing industry.

The invention discloses application of rhamnolipid as a biological cleaning agent. The rhamnolipid is mainly applied to cleaning membrane modules, vegetables and fruits, and has a cleaning efficiency higher than the present ordinary membrane cleaning agents as well as fruit and vegetable cleaning agents. In the cleaning agent disclosed by the invention, the rhamnolipid can be used only or is compounded with other chemical cleaning agents, and an obvious cleaning effect is achieved; in addition, as cleaning conditions are moderate, a cleaned object cannot be damaged; and the cleaning agent has a very good characteristic of environment friendliness.

The invention discloses a manufacture method for rhamnolipid that includes the following steps: filtering fungus, inoculating into culture medium after taking dipping preprocess to the sample getting from oil field sewage or grease, inoculating on blood agar plate for culturing, selecting single colony, inoculating on blue agar plate for culturing, selecting the colony having blue halo to the bevel for microbial conserving; fermenting the primary screening strain in fermenting culture medium, testing the oil drainage activity, emulsibility and fermenting liquid surface tension, selecting target strain; taking ferment to the selected strain, and optimizing the fermenting culture medium, fermenting condition and strain to gain rhamnolipid fermenting liquid; distilling rhanmolipid raw product from the rhamnolipid fermenting liquid. The invention has high yield, good heavy hydrocarbon degradation effect, and improves the selecting accuracy.

The invention belongs to the technical field of biological immobilization and environmental protection and particularly relates to an immobilized microorganism particle for degrading petroleum in low-concentration oily water. The immobilized microorganism particle consists of embedding materials and activated sludge, wherein the embedding materials and the activated sludge are mixed according to a mass ratio being 0.5-2:1, the embedding materials consist of carriers, SiO2, surface active agents and cross-linking agents, the carriers are polyvinyl alcohol and sodium alginate, and the surface active agents are Tween 80 and/or rhamnolipid, wherein the carrier concentration is 8 percent to 12 percent (W/V), the SiO2 concentration is 1.5 percent to 3 percent (W/V), the final concentration of the Tween 80 in the surface active agents is 100 to 200mg/L, and the final concentration of the rhamnolipid is 100 to 180mg/L. The immobilized microorganism particle prepared by the method has the advantages that the interface tension can be effectively reduced, the oil/water interface area is increased, the biological degradation processes of oil pollutants are obviously promoted, and the deoiling effect of microorganisms is greatly improved.

The invention relates to a method for remediation of contaminated soil, in particular to a method for combined remediation of DDTs-PAHs combined contaminated soil. Biosurfactant rhamnolipid is combined with efficient mixed degrading bacteria to strengthen and promote organic chloride pesticide DDTs and PAHs left in facility agriculture soil for decomposition and conversion; and meanwhile low-accumulation vegetable crops are planted, and microbial growth and degradation of the organic contaminant DDTs and PAHs are further strengthened through the plant root system metabolism and the chemical stimulation function of root exudates. The method has the beneficial effects that damage to the environment is small, no secondary contamination is generated, cost is low, large-scale operation is easy, and the production and economic income are not influenced; the method is a safe and effective in-situ remediation technology capable of achieving remediation and production at the same time; and the method is suitable for remediation of the intermediate and low contamination facility agriculture soil where crops are still planted.

The invention relates to a method for increasing the rhamnolipid with pseudomonas aeruginosa; the method comprises steps of the activation and enrichment of the pseudomonas aeruginosa, aerobic fermentation culture, high-speed centrifugal sterilization, extraction and air drying of the fermentation liquid. The invention, with added vector, enlarges the accretion of the pseudomonas aeruginosa to increase the output of the rhamnolipid, which is beneficial to the management of environmental pollution.

Provided is a host cell comprising a rhlA gene or an ortholog thereof, under the control of a heterologous promoter and a rhlB gene or an ortholog thereof, under the control of a heterologous promoter. The host cell is capable of achieving a carbon yield of more than 0.18 Cmol rhamnolipid/Cmol substrate. Provided is also a method of producing rhamnolipids, employing such a host cell.

To provide safe and easy means for preventing or treating diseases of birds and mammals, in particular, livestock. In particular, to provide means for preventing or treating an infectious disease caused by a Gram-positive bacterium. In addition, to improve fermentation in the rumen of a ruminant animal, to contribute to suppression of the generation of greenhouse gas, and to increase the feed efficiency. Mannosylerythritol lipids (MEL) and/or rhamnolipids are given to birds or mammals.

A process for producing rhamnolipids involving culturing Pseudomonas chlororaphis strain NRRL B-30761 in a first aqueous culture medium containing about 0.3% NH₄H₂PO₄, about 0.2% K₂HPO₄, about 0.2% carbon source, about 0.5 mg/L FeSO₄, and about 0.1% MgSO₄ for about 24–about 48 hours at about 25°–about 30° C. with orbital shaking, and then culturing Pseudomonas chlororaphis strain NRRL B-30761 in a static second aqueous culture medium containing per liter about 2% carbon source, about 0.7 g KH₂PO₄, about 0.9 g Na₂HPO₄, about 2 g NaNO₃, about 0.4 g MgSO₄.7H₂O, and about 0.1 g CaCl₂.2H₂O for at least about 72 hours at about 20°–about 23° C., wherein the first and second aqueous culture medium contains only one source of carbon.

The invention discloses a biologic emulsifier for livestock feed. The biologic emulsifier is characterized by being prepared by two or more than two of sophorolipid, rhamnolipid and trehalose. The sophorolipid, the rhamnolipid and the trehalose and the like in the emulsifier are all secondary metabolites generated by microbial fermentation, can be naturally metabolized and degraded, do not cause residues in animal bodies, and do not influence quality of animal products. The biologic emulsifier can remarkably improve livestock fat digestion utilizing rate, improves feed reward, reduces livestock blood cholesterol and triglyceride, remarkably improves livestock production performances, improves carcass quality, improves body immune function, reduces morbidity and mortality, and remarkably improves economic returns.

The invention discloses an oxidized graphene-rhamnolipid composite material which is black gray powder and is of a laminar multi-hole nano structure. The invention further discloses a preparation method of the composite material. The preparation method includes the following steps that oxidized graphene is added into a dispersing agent, and oxidized graphene mixed liquor is formed after ultrasonic dispersion; rhamnolipid, 1-ethyl-(3-dimethyl amino propyl) carbodiimide hydrochloride and 4-dimethylamino pyridine are added into the mixed liquor, then the ultrasonic reaction is performed, and a certain amount of absolute methanol is added and mixed for generating sediments; the sediments are washed and centrifuged, and finally the oxidized graphene-rhamnolipid composite material is prepared through vacuum freeze drying. The invention further relates to application, in aspect of cationic dye absorption, of the composite material prepared through the method. The prepared composite material is low in biological toxicity and not prone to agglomeration, the preparation method is simple and easy to carry out, and in the process of the cationic dye absorption, the prepared composite material has the advantages of being free of secondary pollution and enabling solids and liquids to be separated easily.

The present invention provides a safe and easy means for preventing or treating diseases of birds and mammals, in particular, livestock. In particular, the present invention provides a means for preventing or treating an infectious disease caused by a Gram-positive bacterium. In addition, the invention aims to improve fermentation in the rumen of a ruminant animal, to contribute to suppression of the generation of greenhouse gas, and to increase the feed efficiency. Mannosylerythritol lipids (MEL) and/or rhamnolipids are given to birds or mammals.

The invention discloses a leaching agent and a leaching method for repairing heavy metal contaminated soil. The leaching agent comprises oxalic acid solutions and rhamnolipid solutions. The leaching method includes the steps: preparing the leaching agent for repairing heavy metal contaminated soil; adding the oxalic acid solutions into the heavy metal contaminated soil to uniformly mix, and filtering leaching liquid after the oxalic acid solutions and the heavy metal contaminated soil are reacted; adding the rhamnolipid solutions into the soil treated by the oxalic acid solutions to uniformly mix, filtering leaching liquid after the rhamnolipid solutions and the soil treated by the oxalic acid solutions are reacted, and repairing the heavy metal contaminated soil. The leaching agent and the leaching method are low in cost, safe, efficient and high in applicability, soil characteristics can not be intensively broken, the step-by-step leached soil can be directly backfilled, and the risk of secondary pollution is avoided.

The invention discloses a heavy metal biological absorbent, a preparation method of the absorbent and an application in treating cadmium-containing wastewater. The heavy metal biological absorbent mainly consists of penicillium simplicissimum bacteria powder after being modified by rhamnolipid. The method for preparing the biological absorbent comprises the following main steps: culturing penicillium simplicissimum in a liquid culture medium, then continuously enlarging and culturing the cultured penicillium simplicissimum, adding the rhamnolipid taken as biosurfactants during the process, collecting fungus balls after the culture, cleaning the fungus balls with inorganic salt solution, freezing and drying the fungus balls to the constant weight and grinding the fungus balls into powder to obtain the heavy metal biological absorbent. In the application in treating the cadmium-containing wastewater, the heavy metal biological absorbent is mainly utilized to remove the cadmium in the wastewater, and the method mainly comprises the following steps: adding the heavy metal biological absorbent into the cadmium-containing wastewater, carrying out oscillatory reaction and completely removing after the filtration. The heavy metal biological absorbent has good absorption effect when being used for treating the cadmium-containing wastewater. The method for removing the cadmium contained in the wastewater has the advantages of simple operation conditions, low cost and high efficiency and is easy to implement.