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Combined restoring method of polycyclic aromatic hydrocarbon contaminated soil

A technology for polluted soil and polycyclic aromatic hydrocarbons, applied in the field of remediation, can solve problems such as polluted soil, and achieve the effects of promoting increase, improving degradation rate and improving micro-ecological environment.

Inactive Publication Date: 2011-02-16
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Technical problem: the purpose of this invention is to address the problem of polycyclic aromatic hydrocarbons polluted soil, to provide a kind of combined repair method utilizing plant-microbe-biological surfactant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The perennial leguminous plant alfalfa was planted in the polluted farmland soil with the total amount of polycyclic aromatic hydrocarbons at 13.5 mg / kg, with a row spacing of 10 cm, and mycorrhizal fungi were inoculated in a flat form at a distance of 5-10 cm from the soil surface Bacteria Glomus scoliosis, the inoculum amount accounted for 5% of the weight of the contaminated soil. After growing for 20 days, inoculate obligate degrading bacteria at a distance of 2-5 cm from the root, the inoculation amount accounts for 4% of the weight of the contaminated soil, and add biosurfactant rhamnolipid at 2-10 cm on the soil surface, The weight ratio of it to the soil is 0.03 g / Kg and mixed evenly. In the conventional field management mode, soil samples were collected for analysis after 90 days.

[0014] The results showed that after 90 days of cultivation, the content of PAHs in the soil was 4.5 mg / kg. Compared with the control, after combined repair, the degradation rate ...

Embodiment 2

[0016] In the polluted farmland soil with a total amount of PAHs of 10.29 mg / kg, the perennial leguminous plant alfalfa was planted with a row spacing of 10 cm, and mycorrhizal fungi were inoculated in a flat form at a distance of 5-10 cm from the soil surface Bacteria Glomus scoliosis, the inoculum amount accounted for 4% of the weight of the contaminated soil. After growing for 20 days, inoculate obligate degrading bacteria at a distance of 2-5 cm from the root, the inoculation amount accounts for 2% of the weight of the polluted soil, and add biosurfactant rhamnolipid at 2-10 cm on the soil surface, The weight ratio of added rhamnolipid to soil is 0.05 g / Kg and mixed evenly. In the conventional field management mode, soil samples were collected for analysis after 90 days.

[0017] The results showed that after 90 days of cultivation, the content of PAHs in the soil was 4.5 mg / kg. Compared with the control, after combined repair, the degradation rate of PAHs in the tested ...

Embodiment 3

[0020] In the polluted farmland soil with a total amount of polycyclic aromatic hydrocarbons of 8.56 mg / kg, the perennial leguminous plant alfalfa was planted with a row spacing of 10 cm, and mycorrhizal fungi were inoculated in the form of tiles at a distance of 5-10 cm from the soil surface. Scotia glaucoma, the inoculation amount accounted for 4% of the weight of the contaminated soil. After growing for 15 days, inoculate the obligate degrading bacteria agent at a distance of 2-5 cm from the root, the inoculum amount accounts for 3% of the weight of the polluted soil, and add biosurfactant rhamnolipid at the soil surface 2-10 cm at the same time, The weight ratio of added rhamnolipid to soil is 0.045g / Kg and mixed evenly. In the conventional field management mode, soil samples were collected for analysis after 90 days.

[0021] The results showed that after 90 days of cultivation, the content of PAHs in the soil was 4.5 mg / kg. Compared with the control, after combined rep...

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Abstract

The invention relates to a combined restoring method of polycyclic aromatic hydrocarbon contaminated soil, comprising the following restoring steps of: planting lucerne as perennial leguminous plants in the polycyclic aromatic hydrocarbon contaminated soil; inoculating a mixed bacterial inoculum comprising arbuscular mycorrhizal fungi, bacillus and flavobacterium; and meanwhile, adding rhamnolipid as a biosurfactant. By rhizosphere effect generated by plant root exudates, the number and the metabolic activity of soil microorganisms are increased; and by inoculating the arbuscular mycorrhizal fungi, the plant growth is promoted, and the root exudates are increased, thereby increasing the root accumulation of organic pollutants and the number of root soil microorganisms. The inoculated high-efficiency polycyclic aromatic hydrocarbon degrading bacteria use plant root secretions as a carbon source and an energy source and can quickly grow and propagate. The polycyclic aromatic hydrocarbon is stripped from soil grains by the biosurfactant, and the bioavailability and the degradability of the organic pollutants are increased.

Description

technical field [0001] The invention relates to the technical field of environmental remediation, in particular to a method for remediating soil contaminated by polycyclic aromatic hydrocarbons. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of persistent organic pollutants that widely exist in the environment and are carcinogenic, teratogenic, and mutagenic, and pose a great threat to the ecological environment, agricultural product safety, and human health. Phytoremediation technology is a green restoration technology developed in recent years, which is mainly used to remove toxic pollutants in the environment. It has the advantages of low investment, simple operation, no secondary pollution, and can be used for large-scale pollution control. However, due to the low bioavailability of polycyclic aromatic hydrocarbons in soil, the efficiency of phytoremediation is often limited in the short term, and the d...

Claims

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Application Information

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IPC IPC(8): B09C1/10B09C1/00
Inventor 尹睿张晶林先贵刘魏魏
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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