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535results about How to "Grow fast" patented technology

Applications of cladosporium sp. Strain and synthetic flora thereof in plant growth promotion and bacterial wilt prevention and control

The invention relates to the technical field of microorganisms, in particular to application of a cladosporium spp. Strain and a synthetic flora thereof in plant growth promotion and bacterial wilt prevention and treatment, the cladosporium spp. HX2 is a new strain, has a good symbiotic relationship with tomatoes, has a good prevention effect on tomato bacterial wilt, and has a good application prospect in plant growth promotion and bacterial wilt prevention and treatment. After the bacterial strain is mixed with congeneric strains LJ1, MS2 and LC3 to prepare a cladosporium bacterial agent, the growth-promoting and disease-resisting capabilities of the bacterial agent are enhanced, and experiments show that the synthetic flora has a good growth-promoting function on tomatoes and fresh ginger, the yield of the fresh ginger is remarkably increased, and the growth vigor of tomato plants is improved; meanwhile, the synthetic flora also has a good prevention and treatment effect on bacterial wilt of tomatoes, fresh ginger and tobaccos, and can improve the bacterial wilt resistance of plants, so that the strain HX2 and the synthetic flora prepared from the strain HX2 can be used as an anti-microbial agent or a raw material for preparing a bio-organic fertilizer in the later period.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI

Application of bottle cladosporium LC3 in crop growth promotion and bacterial wilt resistance

The invention relates to the technical field of microorganisms, in particular to application of Cladosporium LC3 in crop growth promotion and bacterial wilt resistance, the strain LC3 belongs to Cladosporium and Cladosporium immonda species, the strain is separated from Guangxi sugarcane rhizosphere soil, experimental evaluation screening finds that the strain has a good growth promotion function on tomatoes and fresh ginger, and the strain can be applied to crop growth promotion and bacterial wilt resistance. The yield of ginger is remarkably increased, and the growth vigor of tomato plants is effectively improved; meanwhile, the strain has a good prevention and treatment effect on tomato and ginger bacterial wilt and has good plant bacterial wilt resistance, and screening verification of the strain provides a new fungicide and a raw material for later-period growth of an anti-microbial agent or a biological organic fertilizer.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI

Bacillus velezensis as well as separation method and application thereof

The invention relates to the technical field of agricultural microbial agents, in particular to bacillus velezensis as well as a separation method and application thereof. The bacillus velezensis is preserved in the China General Microbiological Culture Collection Center, the preservation date is May 17, 2024, the preservation number is CGMCC No.30687, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the postcode is 100101, and the bacillus velezensis is classified and named as bacillus velezensis. The bacillus velezensis separated from the soil of the Ningboo garden of the Academy of Agricultural Sciences in Yitai City of Shandong Province or the fermentation inoculant thereof can be simultaneously used as a preparation raw material of a growth promoting agent, a yield increasing agent, a soil conditioner and a bio-organic fertilizer, and has multiple application scenes.
Owner:YANTAI ACAD OF AGRI SCI SHANDONG PROVINCE (YANTAI BRANCH OF SHANDONG ACAD OF AGRI SCI)

BBM-added bamboo powder biodegradable material and application thereof in beer bottles

The invention relates to the technical field of biodegradable materials, and particularly discloses a BBM-added bamboo powder biodegradable material and application thereof in beer bottles. The BBM-added bamboo powder biodegradable material is prepared from the following raw materials in parts by mass: 10 to 20 parts of poly (butylene succinate), 10 to 30 parts of poly (butylene adipate-co-terephthalate), 10 to 20 parts of bamboo powder, 1 to 3 parts of dopamine hydrochloride, 0.1 to 0.5 part of n-butylamine, 1 to 2 parts of hexadecyl trimethyl ammonium bromide, 1 to 5 parts of tetraethyl orthosilicate and 1 to 2 parts of tetrabutyl titanate. 1-2 parts of 3-aminopropyltriethoxysilane, 1-2 parts of an antioxidant and 1-2 parts of a plasticizer. The BBM obtained by the invention has excellent mechanical properties, improves the anti-ultraviolet intensity of a degradable material to a certain extent, and has very good biodegradability.
Owner:SHANTOU LEISHI PLASTIC TECH CO LTD

Ecological seed-carrying slurry for slope of mine dump, preparation method and application thereof

The present invention relates to the technical field of ecological concrete slurry, and specifically to an ecological seed-carrying slurry for the slope of a mine dump, a preparation method and its application. Aiming at the special environmental characteristics of the Inner Mongolia mining area, the present invention prepares a new type of artificial aggregate containing growth domains and seeds, which is composed of a three-layer structure, each layer having a specific function, so as to achieve the purpose of promoting the germination of multi-source species, plant growth, providing nutrition and improving the soil environment. The slurry can not only effectively utilize solid waste and reduce costs, but also adopts in-situ point-type layered hole sowing to shorten the vegetation restoration cycle in the ecological restoration of the slope, greatly improve the survival rate of tree and shrub seed sources, reduce the number of watering times, save restoration costs, and reduce the workload of management. While effectively promoting vegetation restoration, it strengthens the stability of the slope, prevents soil and water loss, slows down runoff and consolidates the soil, providing an efficient and sustainable solution for the ecological restoration of green mines in Inner Mongolia.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY

Method for afforestation in arid region

The invention provides a method for afforestation in arid regions, and relates to the field of afforestation. The method comprises the following specific steps: S1, digging holes; s2, fertilizer is put into one side in the pit; s3, a barrel-shaped first thin film is laid in the pit; s4, the pit is backfilled with soil for the first time; s5, adding water-retaining agent dry particles into the pits, and watering the pits until the water-retaining agent dry particles absorb water and expand into gel solids; s6, the center position of the gel solid in the pit is backfilled with soil for the second time; S7, the saplings are planted in the soil pile stand columns backfilled for the second time at the same time; in the planting process, the soil backfilled for the second time is tamped downwards and makes full contact with the soil backfilled for the first time, so that the gel solid overflows upwards from the gaps and wraps the soil backfilled for the second time and the planted saplings, and the surface of the gel solid is covered with the remaining soil backfilled for the second time; s8, the surface of the soil backfilled for the second time is covered with a thin film for the second time; and S9, the remaining soil is backfilled for the third time and covered with the film for the second time, and the soil is tamped. The method can improve sapling survival rate, reduce cost and promote sapling growth.
Owner:SICHUAN SIX WALNUT SEEDLINGS TECHNOLOGY CO LTD

Lifting type heating mantle

A lifting type heating mantle comprises a heating mantle body, a supporting frame, a lifting mechanism and an electric control cabinet, the heating mantle body is horizontally arranged over a foaming furnace outlet guide roller set in the front-back direction, and the supporting frame is fixedly installed on a rack of the foaming furnace outlet guide roller set and stretches across the upper portion of the heating mantle body. The lifting mechanism is installed on the supporting frame and drives the heating cover body to move up and down, smoke exhaust ducts are arranged on the inner sides of a left side plate and a right side plate of the heating cover body in the front-back direction, smoke exhaust ports are formed in the left front side and the right front side of the heating cover body, and the smoke exhaust port in the left side communicates with the smoke exhaust duct in the left side. The smoke exhaust port on the right side is communicated with the smoke exhaust flue on the right side, the two smoke exhaust ports are connected with a main smoke exhaust pipe of the foaming furnace through hoses, a PLC is arranged in the electric control cabinet, and the PLC is in signal connection with the lifting mechanism. The smoke exhaust device is scientific in design, smoke escape can be avoided, dependence on a power source is reduced, and fire accidents are avoided.
Owner:HENAN YILONG IND

Marine invertebrate cell culture medium

The invention relates to the technical field of cell culture media, in particular to a marine invertebrate cell culture medium. Comprising the following components in parts by weight: 0.5 to 1 part of a compound supplement, 1 to 4 parts of glucose, 0.3 to 0.5 part of L-glutamine, 0.01 to 0.05 part of vitamin, 10 to 15 parts of fetal calf serum, 0.02 to 0.04 part of penicillin, 0.01 to 0.03 part of trehalose, 0.3 to 0.7 part of a sodium chloride osmotic pressure regulator, 0.5 to 1 part of inorganic salt and 10 to 25 parts of a sodium bicarbonate buffer agent, wherein the compound supplement is prepared by mixing meson, diatom and disodium cyclohexane diamine tetraacetate according to the mass ratio of 1: (0.2-0.6): (0.1-0.5), the application range of the culture medium is widened by adopting the compound supplement, the meson and the diatom are added into the cell culture medium after enzymolysis, and compared with a chemically synthesized culture medium, the culture medium better meets the requirements of marine invertebrate cells; therefore, cell growth is effectively promoted, and the purposes of rapid growth and stable passage are achieved.
Owner:青岛海科生物技术有限公司

Application of OsATS3 gene in regulation and control of rice flooding stress tolerance

ActiveCN119220591Agrow fastImprove seedling ratePlant peptidesFermentationBiotechnologyRadicle
The invention discloses an application of an OsATS3 gene in regulation and control of rice flooding stress tolerance. According to the invention, an OsATS3 gene is constructed to edit a knockout vector, an agrobacterium tumefaciens-mediated method is adopted to transform rice calluses, and a knockout strain of the OsATS3 gene is obtained through screening, and the knockout strain of the OsATS3 gene shows a phenotype that after seeds germinate under a waterflooding condition, seedling root systems are developed, seedling growth of overground parts of seedlings is faster, the growth speed of first leaves is faster, and the seedling rate is high; the method has great significance in solving the problems of slow seedling growth, inhibited radicle development, low seedling rate and the like in the rice direct seeding process, and has a wide application prospect in plant breeding.
Owner:AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI

Recombinant thallus, construction method thereof and application of recombinant thallus in production of xanthoxylin WGX-50

The invention relates to a recombinant thallus, a construction method of the recombinant thallus and application of the recombinant thallus in production of xanthoxylin WGX-50, and relates to the technical field of bioengineering. A recombinant plasmid containing a phenylalanine ammonialyase gene PAL with a nucleotide sequence as shown in SEQ ID NO.2 and a cinnamic acid coenzyme A ligase gene CNL with a nucleotide sequence as shown in SEQ ID NO.1 and a recombinant plasmid containing a tyramine N-hydroxycinnamoyl transferase gene THT with a nucleotide sequence as shown in SEQ ID NO.3 are introduced into the recombinant plasmid. A phenylalanine ammonialyase gene PAL, a cinnamic acid coenzyme A ligase gene CNL and a tyramine N-hydroxycinnamoyl transferase gene THT are overexpressed in escherichia coli, so that a biological synthesis path of xanthoxylin WGX-50 is formed by taking 3, 4-dioxomethyl phenylethylamine as a substrate for the first time, a foundation is laid for large-scale industrial production, and the method has a wide application prospect. The method has important economic value and social benefit.
Owner:ANHUI UNIV

Large-scale breeding method for fry of heterophysogobio boulengeri

The invention provides a large-scale breeding method of heterophyllobotia tapeinosoma fry, which comprises the following steps: temperature-controlled incubation of a breeding pond, still water heating breeding and ecological cycle breeding, establishes an intensive breeding mode of fry with high density, fast growth and healthy fish body, solves the problem of low survival rate in the breeding process of heterophyllobotia tapeinosoma, and improves the breeding efficiency of heterophyllobotia tapeinosoma. The breeding scale and the breeding efficiency are practically improved, and a key technical support is provided for large-scale enhancement and release.
Owner:CHINESE STURGEON RES INST OF CTG +1

Shanghage seaweed culture bed and culture system

The utility model discloses a Shanghaeria seaweed culture bed and culture system, wherein the Shanghaeria seaweed culture bed comprises a support and a final singling structure, the support comprises a frame and a plurality of support tubes, the plurality of support tubes are connected with the bottom of the support, and the frame is provided with a plurality of openings; the final singling structure is arranged in the frame and comprises a first mesh and a second mesh, and the first mesh and the second mesh are detachably connected with the frame; and a gap is formed between the first mesh and the second mesh. According to the technical scheme, the supporting pipes are arranged, so that the solieria seaweed seedlings are separated from the bottom of the pond and can grow in a suspension mode, and the yield and the quality are improved; meanwhile, the first mesh and the second mesh are detachably connected with the frame, so that the first mesh and the second mesh can be conveniently connected and detached; an opening is formed in the frame and used for allowing a water body to enter the frame, so that the solieria seaweed culture bed can stably sink to the bottom of the water body; the solieria seaweed culture bed is simple in structure, low in price and convenient to use.
Owner:SOUTHERN MARINE SCIENCE & ENGINEERING GUANGDONG LABORATORY (ZHANJIANG)

Sucrose isomerase mutant and application thereof in production of isomaltulose

ActiveCN119432953AIncrease enzyme activityIncreased specific enzyme activityBacteriaTransferasesIsomeraseMicrobiology
The invention discloses a mutant with improved enzyme activity of sucrose isomerase and application of the mutant, and belongs to the technical field of genetic engineering and enzyme engineering in molecular biology. A known sucrose isomerase parent is subjected to molecular modification by utilizing a site-directed mutagenesis technology to obtain mutant enzymes T522S / K340D, D222H / K340D, G467N / K340D, T522S, K340D, D222H and G467N, and the catalytic activity of the series of mutants provided by the invention is increased by 10%-25% compared with that of original bacteria; the isomaltulose conversion rates of the combined mutants T522S / K340D, D222H / K340D and G467N / K340D are respectively increased by 2.3%, 1.5% and 1.3% compared with those of original bacteria, and the strain has good stability, can be used for biological preparation of isomaltulose and has potential application prospects.
Owner:JIANGNAN UNIV

Anti-scattering water-guided laser cutting fluid as well as preparation method and application thereof

The invention provides anti-scattering water-guided laser cutting fluid as well as a preparation method and application thereof. The anti-scattering water-guided laser cutting fluid comprises the following components in parts by weight: 0.1-1 part of an anti-scattering agent; 0.01 to 0.1 part of a foam discharging agent; 1-5 parts of a refractive index regulator; and 80-99 parts of ultrapure water. The anti-scattering agent is a polymer; the foam removing agent is one or more of hydroxymethyl cellulose, hydroxy propyl cellulose, sodium lignin sulfonate, potassium lignin sulfonate and naphthalene sulfonic acid formaldehyde condensate sodium salt; the refractive index regulator is an iodine-containing compound and / or a fluorine-containing compound. According to the anti-scattering water-guided laser cutting fluid, light beam scattering and energy loss in the water-guided laser cutting process can be remarkably reduced, and the cutting precision and efficiency are improved. The anti-scattering water-guided laser cutting fluid has very good application prospects and large-scale industrial popularization potential in the field of water-guided laser cutting.
Owner:DALIAN AOSHOU TECH CO LTD

Method for promoting tillering of zizania latifolia by using sulfopeptide

The invention provides a method for promoting tillering of zizania aquatica by using sulfopeptide, and solves the technical problem that the tillering number of zizania aquatica is small in the prior art. The invention provides a method for promoting tillering of zizania aquatica by utilizing sulfopeptide and application of the sulfopeptide. The method comprises the step of smearing zizania aquatica seedlings with the sulfopeptide. The Zizania aquatica seedlings are coated with the sulfokine, division and proliferation of Zizania aquatica cells can be promoted, more branches can be generated in the tillering stage of the Zizania aquatica, and therefore the tillering and yield of plants are increased. Meanwhile, by smearing the sulfokine on the zizania aquatica seedlings, growth of the zizania aquatica can be promoted by influencing development and functions of plant root systems and improving nutrient absorption of plants and transfer efficiency of the plants in the plants, leaves in the early stage can grow rapidly, the leaf area is increased, and therefore photosynthesis is improved, photosynthesis efficiency is improved, and the yield of the zizania aquatica is increased.
Owner:ZHEJIANG NORMAL UNIV

Cultivation method for shortening cultivation period of trachinotus ovatus

The invention provides a culture method for shortening the culture cycle of trachinotus ovatus, which specifically comprises the following steps: (1) setting and dividing different culture areas, and spraying compound bacteria liquid in each culture area before fry casting; (2) fry putting: healthy trachinotus ovatus fries are screened and put into each culture area; (3) feed feeding: feeding different nutrient materials in different culture areas; (4) cultivation management; (5) daily management: arranging a light source lamp above the breeding area. By means of the breeding method, the breeding period of the trachinotus ovatus is obviously shortened, the situation that the breeding environment is converted in the breeding process of the trachinotus ovatus is avoided, and the situation that the trachinotus ovatus generates stress reaction or is infected with various diseases, and consequently the breeding cost is increased is avoided. The golden pomfret obtained through the breeding method is high in growth speed, the immunity is improved, the morbidity and the mortality rate are reduced in the breeding process, and the flavor quality of fish products is improved.
Owner:HAINAN CHENHAI AQUATIC CO LTD

Floating vapor phase growth method and device of single-walled carbon nanotube and application of floating vapor phase growth method and device

The invention discloses a floating vapor phase growth method and device for a single-walled carbon nanotube, and application of the floating vapor phase growth method and device. The floating vapor phase growth method comprises the following steps: forming a nickel-titanium alloy catalytic core in a first channel, and forming carbon atom gas in a second channel; the nickel-titanium alloy catalyst core and the nickel-titanium alloy catalyst core meet each other and grow to form a single-walled carbon nanotube, and the nickel-titanium alloy catalyst core has a close-packed hexagonal lattice structure. The nickel-titanium alloy with a close-packed hexagonal structure is used as a catalytic core and corresponds to a six-membered ring carbon atom structure in the single-walled carbon nanotube, rapid growth of the single-walled carbon nanotube can be selectively initiated, formation of a double-walled carbon nanotube or even a multi-walled carbon nanotube is inhibited, and the catalytic performance of the single-walled carbon nanotube is improved. And finally, a single-walled carbon nanotube product with high yield, high purity and high crystallinity is obtained. Besides, carbon nanotubes with different characteristics can be formed when different carbon sources are matched, so that the proportion of metal and semiconductor in the product can be adjusted by adjusting the category of the carbon sources, and carbon nanotube products with different conductive characteristics can be obtained in a targeted manner.
Owner:SUZHOU NAPLES NANOMATERIALS CO LTD

Pseudomonas and application thereof in deodorization of kitchen waste

The invention discloses a pseudomonas strain and application thereof in kitchen waste deodorization, and belongs to the technical field of environmental microorganisms, the strain is classified and named as Pseudomonas sp., is preserved in China General Microbiological Culture Collection Center on April 15, 2024, has a preservation number of CGMCC No.30345, is preserved in No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, and has a preservation number of CGMCC No.30345. According to the present invention, the Chinese Academy of Sciences Institute of Microbiology, the postcode is 100101, after the application, the strain can efficiently remove hydrogen sulfide, ammonia gas, VOCs and other components in the malodorous gas, and the new efficient and environmentally-friendly strain resource is provided for solving the air pollution problem caused during the garbage waste treatment process;
Owner:JIANGSU ECOLOGICAL ENVIRONMENT TECHNOLOGY RESEARCH INSTITUTE CO LTD +2

Laboratory plant hydroponic device for collecting root system secreted ions and collecting method

The invention provides a laboratory plant hydroponic device for collecting root system secreted ions and a collecting method, the laboratory plant hydroponic device for collecting root system secreted ions comprises a box body, the box body is provided with an incubator, the incubator comprises an inner box and an outer box, and the bottom of the inner box is provided with a root system secreted ion collecting device; the invention provides a collecting method for collecting root system secreted ions, which comprises the following steps: on the basis of a laboratory plant hydroponic device for collecting the root system secreted ions, part of plant root systems enter a root system secreted ion collecting device, and plant root system secretions are collected from the root system secreted ion collecting device; adding a buffer solution and a methanol solvent into a root exudate collecting tank, eluting to obtain a plant root exudate ion solution, and freeze-drying to obtain root exudate ions. By utilizing the device and the method provided by the invention, the ions secreted by the root system can be efficiently collected, and the culture box arranged in the box body can realize a repeated control group and a treatment group required by an experiment.
Owner:NANTONG UNIV

Lactobacillus crispatus capable of efficiently inhibiting alpha-glucosidase and application of lactobacillus crispatus

The invention discloses lactobacillus crispatus capable of efficiently inhibiting alpha-glucosidase and application of the lactobacillus crispatus. The lactobacillus crispatus JQH7 strain provided by the invention is separated and identified from human excrement, has relatively strong self-aggregation capability which can reach about 96% in 24 hours, can be better adhered to biological or non-biological surfaces, and is beneficial to effective colonization in an intestinal environment. Meanwhile, the JQH7 strain has certain antioxidant capacity, and the scavenging rate of a bacterial suspension of the JQH7 strain on DPPH free radicals is 49.18%; the JQH7 strain has the capability of efficiently inhibiting alpha-glucosidase, the effect of the JQH7 strain is equivalent to that of a clinical medicine acarbose, and compared with the acarbose, the JQH7 strain is safe in source, free of side effects, low in price, easy to obtain and easy to prepare. The invention provides more sources and choices for preparing preparations for efficiently inhibiting alpha-glucosidase and hypoglycemic products, provides a new thought for adjuvant therapy of diabetes, and is beneficial to improving and controlling the blood sugar of diabetics.
Owner:HUNAN CARE-U BIOTECHNOLOGY CO LTD

Bacillus subtilis and application thereof

The invention discloses bacillus subtilis and application thereof, and relates to the technical field of microorganisms, the bacillus subtilis S1-17L provided by the invention is preserved in the China General Microbiological Culture Collection Center, the strain preservation number is GGMCC NO: 31323, the preservation date is July 16, 2024, and the 16S rDNA sequence of the bacillus subtilis S1-17L is as shown in SEQ ID NO.1; the strain can secrete a plurality of hydrolases including protease, phosphatase and cellulase, has relatively high capacity of dissolving insoluble phosphorus in soil and a plurality of biological functions, is a growth-promoting and environment-friendly strain, and lays a foundation for research and development of efficient and green microbial fertilizers.
Owner:ZHEJIANG FORESTRY UNIVERSITY +1

Tissue culture and rapid propagation method for bamboo reed

The invention provides a method for tissue culture and rapid propagation of bamboo reed. The method comprises the following steps: (1) taking, disinfecting and sterilizing an explant; (2) induction culture: an induction culture medium is MS + 4.0 mg / L 6-BA + (1-1.5) mg / L IBA; (3) proliferation culture: a proliferation culture medium is MS + 7mg / L 6-BA + (0.5-1) mg / L IBA, and robust tissue culture rootless seedlings are obtained; (4) rooting culture, wherein a rooting culture medium is 1 / 2MS + 0.2 mg / LNAA; (5) seedling hardening: directly cleaning a rooting culture medium, transplanting the rooting culture medium into a nutrition cup for seedling raising, using a 50-hole deep hole tray container as the nutrition cup, and hardening the seedling without covering a sunshade net by using a cultivation nutrition medium of perlite, vermiculite and turf in a weight ratio of 3: 1: 1 to obtain seedlings. The tissue culture and rapid propagation method is particularly suitable for the space bamboo reed, the axillary buds are high in germination rate and good in growth vigor after induction culture, the multiplication coefficient of multiplication culture is high, the rooting time is early, the number of roots is large, the rooting rate is high, the roots grow fast, and obtained tissue culture seedlings are robust, neat in growth vigor, fast in seedling recovering and forming and high in transplanting survival rate.
Owner:AEROSPACE LVPENG BIOTECHNOLOGY (BEIJING) CO LTD

Quickly separated microneedle patch as well as preparation method and application thereof

The invention discloses a rapidly-separated microneedle patch as well as a preparation method and application thereof. The microneedle patch comprises a needle tip prepared from a needle tip liquid and a separation backing prepared from a separation substrate liquid, the needle tip liquid is composed of a drug-containing clathrate compound solution, a photoinitiator and a hypotonic agent; the drug-containing clathrate compound is prepared by covalently connecting a beta-cyclodextrin conjugated polymer material with an angiogenesis promoting drug and then clathrating a 5alpha-reductase inhibitor, the separation substrate liquid is composed of a polymer material, a gas initiator and a solvent; the gas initiator is composed of an acid source and an alkali source; the rapid separation microneedle is high in separation and permeation promotion performance, the hair follicle microenvironment is improved from multiple aspects, hair regeneration is promoted, and the rapid separation microneedle has wide application prospects in treatment of androgen alopecia.
Owner:JINAN UNIVERSITY

Rapidly separated microneedle patch and preparation method and application thereof

The invention discloses a fast-separation microneedle patch and a preparation method and application thereof. The microneedle patch comprises a needle tip prepared from a needle tip liquid and a separation backing prepared from a separation base liquid; the needle tip liquid consists of a drug-containing inclusion compound solution, a photoinitiator and a hypotonic agent; the drug-containing inclusion compound is prepared by first covalently linking a β-cyclodextrin conjugated polymer material to an angiogenesis-promoting drug and then encapsulating a 5α-reductase inhibitor; the separation base liquid consists of a polymer material, a gas initiator and a solvent; the gas initiator consists of an acid source and an alkali source; the fast-separation microneedle of the invention has strong separation and penetration-promoting performance, improves the hair follicle microenvironment from multiple aspects to promote hair regeneration, and has broad application prospects in the treatment of androgenic alopecia.
Owner:JINAN UNIVERSITY

Establishment method and application of soybean NLRs disease-resistant gene rapid identification system

The invention relates to an establishment method and application of a soybean NLRs disease-resistant gene rapid identification system. The establishment method comprises the following steps: constructing an expression vector with pathogenic microorganism effect protein and an expression vector carrying soybean candidate NLRs disease-resistant genes; respectively adding into an agrobacterium GV3101 strain, and carrying out electrotransformation to obtain two carrier bacterial liquids; a pathogen effector and an NLRs anti-disease gene from soybean are simultaneously expressed in two-week-old cowpea pair cotyledons through an agrobacterium-mediated transient expression system, and after the cowpea pair cotyledons are placed at 22 DEG C for 5 days, the allergic necrosis phenotype on the leaves is observed to judge whether the NLRs are key anti-disease genes for identifying corresponding pathogenic microorganism effector proteins or not. According to the invention, cowpea is compatible with more soybean NLRs disease-resistant genes capable of identifying pathogenic microorganisms through indirect regulatory factors, so that the cowpea is more suitable for high-throughput screening of the soybean NLRs disease-resistant genes, and the cloning time of the traditional soybean NLRs disease-resistant genes is greatly shortened; and an important technical system is provided for large-scale excavation of the soybean NLRs disease-resistant gene and subsequent soybean disease-resistant breeding.
Owner:NANJING AGRICULTURAL UNIVERSITY

Multi-stage treatment device for industrial wastewater

The invention belongs to wastewater treatment equipment, and particularly relates to a multi-stage treatment device for industrial wastewater, which comprises a treatment pond, a rotating motor connected to a bracket at the upper end of the treatment pond, a rotating shaft at the lower end of the rotating motor extending to the lower end of the treatment pond, stirring blades arranged on the rotating shaft, a drain pipe arranged on one side of the treatment pond, and a vertical pipe arranged at the lower end of the treatment pond, a switch valve is arranged on the vertical pipe, a straight ladder is arranged on one side of the treatment tank, and the rotating shaft is sequentially provided with a stirring blade, a bubble type aerating assembly, a rotating blade assembly, a feeding mechanism, a drawing type aerating mechanism and a foam scraping assembly from bottom to top; according to the device, the stirring effect is improved, activated sludge is added through the feeding mechanism to be better combined with internal wastewater for reaction, the arranged bubble type air-entrapping assembly, a drawing type aeration mechanism and stirring blades are matched with an external air pump for aeration treatment, and a plurality of oxygen-entrapping devices are arranged, so that the wastewater is better contacted with oxygen, the wastewater treatment effect is improved, and the reaction time is shortened.
Owner:ZHONGZHI MASCH (LINYI) CO LTD

Multi-cylinder single-action reciprocating fertilization pump

The invention discloses a multi-cylinder single-action reciprocating fertilization pump, and belongs to the technical field of agricultural mechanical equipment. The modularized pump body is formed by combining multi-cylinder pump bodies, the modularized pump body is installed on the modularized pump head body, a piston element is arranged in each working cavity corresponding to the multi-cylinder pump body, the stroke volumes of the multiple piston elements in the corresponding working cavities are equal, and phase angles are staggered by 0-180 degrees; a liquid inlet and a liquid outlet which are communicated with the corresponding working cavities of the multi-cylinder pump body are formed in the modularized pump head body; the bidirectional offset crank connecting rod mechanism is provided with an optimal transmission angle, and the bidirectional offset crank connecting rod mechanism serves as a driving power end and is in driving connection with a piston element of the three-cylinder pump body so as to drive the piston element to do reciprocating rectilinear motion in the corresponding working cavity; and the valves are mounted at the liquid inlet and the liquid outlet. The multi-cylinder single-action reciprocating type fertilization pump has the advantages of being excellent in force transmission performance, flexible in combination, high in transmission efficiency and the like.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Electric heat tracing straight flow type throttle valve

The utility model discloses an electric heat tracing straight flow type throttling valve. An inlet channel and an outlet channel are formed in the two side walls of the valve body correspondingly, and a first mounting hole is formed in the top of the valve body. A second mounting hole corresponding to the first mounting hole is formed in the valve cover; the mounting assembly is arranged on the side, away from the valve body, of the valve deck and provided with a third mounting hole corresponding to the second mounting hole. A first input end and a second input end of the temperature controller are electrically connected to the power supply and the temperature sensor respectively; the end, away from the temperature controller, of the first electric tracing band is wound around the outer wall of the valve body and then connected to the input end of the anti-explosion three-way junction box, the two output ends of the anti-explosion three-way junction box are connected to the second electric tracing bands corresponding to the output ends of the anti-explosion three-way junction box respectively, and the two second electric tracing bands are wound around the outer wall of the valve body and then connected to the anti-explosion tail end junction boxes corresponding to the second electric tracing bands. Medium solidification or unsmooth flowing can be effectively prevented, and abrasion and damage of a sealing face formed by the opening and closing assembly and the valve seat are reduced.
Owner:XIAN PUMP & VALVE GENERAL FACTORY CO LTD

Duplex stainless steel containing rare earth elements and preparation process of duplex stainless steel

The invention discloses duplex stainless steel containing rare earth elements and a preparation process of the duplex stainless steel. The duplex stainless steel comprises the following components in percentage by weight: less than 0.04% of C, 0.2-0.8% of Si, 0.8-1.5% of Mn, less than or equal to 0.005% of S, less than or equal to 0.02% of P, 22.0-27.0% of Cr, 5.0-7.0% of Ni, 3.0-4.0% of Mo, 0.24-0.32% of N, 0.002-0.005% of B, 0.05-0.10% of mischmetal and the balance of iron and other inevitable impurities. The preparation method comprises the steps of metal smelting; adding alloy for refining; mixed rare earth with the oxygen content smaller than 1000 ppm is added in batches; pouring under the protection of argon; rolling is conducted; and carrying out solution treatment. The method has the characteristics that the purity, the corrosion resistance, the hot working performance and the yield of the duplex stainless steel are improved.
Owner:ZHEJIANG FUGANG METAL PROD CO LTD +1

Culture method for promoting seed germination and seedling growth of cercis chinensis

The invention discloses a culture method for promoting germination of cercis chinensis seeds and growth of seedlings, which comprises the following steps: accelerating germination: soaking the cercis chinensis seeds in a seed soaking solution at normal temperature to treat for 3-4 hours, accelerating germination of the soaked cercis chinensis seeds for 5-9 days, and taking the germinated cercis chinensis seeds; sowing: dibbling the germinated cercis chinensis seeds in a sowing matrix, covering the seeds with 1-2cm of the sowing matrix, keeping the moisture of the sowing matrix at 50-65%, and separating cercis chinensis seedlings with 1-2 true leaves from the sowing matrix when the cercis chinensis seeds grow 1-2 true leaves; seedling cultivation: transplanting the 1-2 true-leaf cercis seedlings into a cultivation substrate, placing the cultivation substrate in a sunshade for cultivation, and applying a root inducing agent and a leaf fertilizer in the cultivation process to induce lateral roots of the cercis seedlings to grow until the height of the cercis seedlings is 50-60cm. Compared with the prior art, the method has the advantages that germination time is shortened by soaking seeds, lateral roots of the Chinese redbud seedlings are promoted to grow and extend by applying the culture medium, the root inducing agent and the leaf fertilizer, root weight and root system quantity are increased, and growth speed of the Chinese redbud seedlings is increased.
Owner:GUANGXI FORESTRY RES INST +1