The invention discloses an
Escherichia coli strain culture method, and belongs to the field of
medicine, the
Escherichia coli strain culture method comprises the following steps: S1, preparing an
asymmetric carbon nitrogen flow regulation type compound culture medium which comprises the following components: 10-25 g / L of a
carbon source component, 4-8 g / L of a
nitrogen source component, 5-10 g / L of a slow release
carbon source component, 5-10 g / L of a
nitrogen source component, 5-10 g / L of a
nitrogen source component and the balance of water, and the
mass ratio of the
carbon source component to the slow release carbon source component is 3: 7; wherein the ratio of
inorganic nitrogen to organic nitrogen is 1: 2, and the microelement liquid is 1-3mL / L; and S2, inoculating a seed solution containing a non-model
escherichia coli strain into the culture medium, and setting initial culture parameters as follows: the temperature is 33 DEG C, the pH value is 6.8, the dissolved
oxygen is maintained at 50%, and the rotating speed is 150rpm. By introducing an
asymmetric carbon-nitrogen regulation strategy and a slow-release carbon
source system, the metabolic environment of non-model
escherichia coli is optimized, and the growth adaptability and stability of the
escherichia coli in an
artificial culture medium are remarkably improved.