568 results about "Artificial culture" patented technology

The invention relates to a method for high-density industrial culture of blood-sucking leeches, which belongs to the technical field of artificial culture of the blood-sucking leeches. The culture method cultures commercial leeches artificially by the steps of selecting a place, building a pond, putting leech seeds into the pond, feeding the leech seeds, preparing cocoon producing beds, producing cocoons and hatching the cocoons, culturing young leeches, living through the winter and the like, and achieves recirculation culture by the steps of producing the cocoons and hatching the cocoons. In the culture process, when the reproduction rate of the cultured leeches goes down after three generations of close breeding, the cultured leeches are mated with wild leeches to achieve the aim of the rejuvenescence of parent leeches. The culture method solves the problems that the changes of the natural environment and the increment of pollutant sources make naturally-growing leech sources decrease day by day and the gradually expanded market demand cannot be satisfied. The culture method for culturing the leeches has less investment but high production. The input of 100 kilograms of the leeches can yield 200 kilograms of the commercial leeches. The culture method can culture the leeches not only by building large vinyl tunnels and ponds, but also by using rural homesteads, yards or vacant houses for building the ponds, and has good economic benefits.

The invention discloses a set of apparatus and a method for culturing Haematococcus pluvialis and converting astaxanthin in large scale. The apparatus consists of a photo-bioreactor system set on the frisket, aeration equipment, culture fluid inoculation equipment and still cell harvesting equipment. Floating or sunken pulse equipment is set on the frisket. The apparatus don't need establishment of artificial culture pool and other institution, but uses natural water body to culture Haematococcus pluvialis in large scale. It is low cost, the culture temperature, illumination and water flow rate is stable, culture fluid flows with each other, aeration is continuous, culture conditions are precedent, which make the growth and increment of Haematococcus pluvialis almost at equal pace. The growth rate of Haematococcus pluvialis can reach to 25%, biomass increase is fast, doubles 3-5 days. The photo-bioreactor can be hoisted into required depth of the water, which solves the contradiction of difference of temperature, illumination and nutrition of Haematococcus pluvialis culture and astaxanthin conversion.

The invention provides a method of full-artificial culturing Cordyceps sinensis, including sampling, strain separating, host insect collecting, breeding and feeding, infecting and continuing feeding, on artificial condition, largely culturing Hirsutella sinensis; feeding host insects in an scale room at low altitude, completing the infection of Hirsutella sinensis to the host insects and sexuality of Cordyceps sinensis. By artificial culture, the host grubs are big and good-quality, and the success rate of infection is high, the infection time is short, the quality is stable and controllable, and the pesticide effect test fully indicates that the pesticide effect of the produced Cordyceps sinensis is equivalent to that of the natural Cordyceps sinensis and it can overcomes the disadvantages of long growing time of natural Cordyceps sinensis and low natural infection ratio, and implements the industrialized production of Cordyceps sinensis.

The present invention provides an apparatus and method of cultivating a plant using a subirrigating method. The apparatus includes a culture medium cell constructed in such a manner that artificial culture medium is packed in a packing material having excellent ventilation and moisture absorptivity to be shaped in a predetermined form, a planting pot which contains the culture medium cell and has holes through which a sufficient amount of nutritive solution and air pass, a cooling / heating device set in the lower portion of the culture medium cell to cool or heat the root of the plant, and a nutritive solution supplying device for automatically supplying the nutritive solution to a plant planted in the culture medium cell placed a distance from one or more nutritive solution containers, thereby supplying the nutritive solution and air to the root of the plant continuously to maintain the optimum growth environment, forcing the plant growth.

The invention relates to an artificial culture method for Portunus trituberculatus in mariculture, and belongs to the technical field of agricultural mariculture. The artificial culture method consists of two parts of technology: 1, an integrated culture regulation method is carried out; and 2, anti-slaughter nets are arranged at the bottom of a pond, 300 nets are arranged in each mu and the slaughter on unshelled crabs by shelled crabs is reduced. The integrated culture regulation method comprises steps of: 1, performing pond regulation: dredging, sterilizing, drying sludge, fertilizing the fond, and laying pipelines at the bottom of the pond and sterilizing the water; 2, performing finite amount of water exchange; 3, regulating the water quality of the culture pond by using composite microorganism agents; 4, reasonable configuring and using aeration facilities; and 5, preparing feeds and reasonably feeding the feeds. Due to the application of the technology, the water quality of the culture pond is improved; the slaughter between the unshelled crabs and the shelled crabs is prevented; and the yield and economic benefit of the portunus trituberculatus are improved.

The invention discloses a culture medium and method for submerged fermentation of inonotus obliquus, relating to a culture medium and method for microbial submerged fermentation, and solving the problems of low yield of traditional inonotus obliquus submerged fermentation mycelia and low output of intracellular polysaccharide and extracellular polysaccharide of the inonotus obliquus as the activeingredient for the submerged fermentation. The culture medium for submerged fermentation is prepared from the following components: carboxymethyl cellulose, glucose, soybean meal, yeast extract, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 and the balance of water. The method for the submerged fermentation comprises the following steps of: (1) preparing a culture medium for the submerged fermentation of inonotus obliquus; (2) inoculating the inonotus obliquus; and (3) carrying out submerged fermentation at two stages. By means of the culture medium and the method for the submerged fermentation of the inonotus obliquus in the invention, higher mycelium yield and intracellular and extracellular polysaccharides can be obtained. The mycelia polysaccharide obtained by the invention is 1.4 times higher than that of wild fruiting bodies, and the content of other active ingredients is higher than or equal to that of the wild fruiting bodies. The culture medium and method provided by the invention can be used for providing a rapid and abundant seed liquid for the large-scale artificial culture of the inonotus obliquus.

An artificial culture method of new balm cricket Penicillium notatum and extraction and application of delivery pains compounds are disclosed. This invention involves declutching and breeding quality new balm cricket Penicillium notatum APC-20 from nature, researching production of the strain, further, defining method of extracting delivery pains compounds to be medicine. The compound is N6-(2- hydroxyethyl) adenosine. Molecular weight is 311.297. Molecular formula is C12H17N5O5. Delivery pains ratio is 99%by the method of mouse test. Principle of delivery pains is particular and cannot induce addiction.

The invention relates to a method for artificial culture and propagation of plant root-knot nematodes and for species preservation. The propagation and preservation of the root-knot nematodes in a lab depend on host plants all the time; the invention places a relatively small amount of root-knot nematode ovums or 2nd instar larvas in a particular dual culture medium rather than host roots and performs a upside-down culture under conditions of constant temperature and hermetic closure, the root-knot nematodes ingest, grow and propagate inside the culture medium and a large amount of nematomorphas can be obtained in a short term; additionally, a culture plate is timely transferred to be located under cold conditions, and dish change and subculture are implemented once within a half year so that the propagation and preservation processes of the root-knot nematodes are carried out without the host plants. The inventive advantages lie in: saving the culture program of the root-knot nematodes to host the plants without losing the infectivity of the root-knot nematodes on sensitive host plants; the employed materials have low costs and the experiment method is easy in operation; the invention provides a simple, convenient and fast propagation method for the species preservation of the root-knot nematodes, in particular for a large number of nematomorphas as required in the related researches of the root-knot nematodes.

The invention relates to a culture raft frame for scallop and mussel culture, which is suitable for artificial culture of marine products including shallow sea scallops and mussels. The culture raft frame comprises a large culture stalk, inflation and exhaust type buoys, a culture cage, pulleys, an inflation and exhaust hose and an air valve core sealing valve, wherein the large culture stalk is connected with the buoys by regulating cables, one buoy is corresponding to a regulating cable, a universal lock catch is arranged below each buoy, a water depth graduation mark is arranged on each regulating cable, the inflation and exhaust type buoys are uniformly arranged on the large culture stalk and connected with the large culture stalk by cables and cable padlocks, water charging and drainage holes are uniformly arranged below the inflation and exhaust type buoys along the circumferential direction, and double pulleys are respectively arranged at both ends of the large culture stalk. The raft frame can realize upward floating and sinking of the large culture stalk within a depth range of 2-12m under the sea level, thereby satisfying various requirements of the cultured marine products on water depth and water temperature in different sea conditions and different seasons.

The invention supplies a high activity wild Chinese caterpillar fungus strain and the artificial planting method. The Chinese caterpillar fungus strain is GDIM_C050423 CCTCC M 206051 that could produce fruiting body with high activity material. The method includes mother strain making, first level liquid strain making, second level liquid strain making, inoculating, artificial plating and gathering. The fruiting body has the feature of rod shaped, no branch or only has short branches at the top, and tip shaped top. It has abundant nutritive value and health protection function. It contains over 39.2% cordycepic acid.

The invention discloses an earthworm culturing material and a preparation method thereof. The earthworm culturing material is prepared through saccharifying leguminosae straws, plant tender green stems and leaves, and rice bran, drying, crushing, and mixing with turf according to the biological characteristics of earthworms, and the finally-obtained earthworm culturing material is neutral to sub-acidic. Raw materials adopted in the invention are rich in stem and leaf crude fibers, and the crude fibers are saccharified to form an absorbent useful soft material rich in nutritional components. The soft material has a loose and soft structure, integrates foods and habitats, can provide a sweet, delicious and dainty food and also provide an favorable inhabiting environment having a high permeability, can effectively urge the earthworm daily weight gain increase and enhance the disease resistance, and has the advantages of wide sources of raw materials, low cost, convenient processing and feeding, and high rate of return as a daily culturing material for the artificial culture of the earthworms.

The invention discloses a method for cultivating the sipunculus nudus in a pond. The sipunculus nudus is low in the level of the food chain, is high in stress resistance, is fast in growth, belongs to the local ecological plant formation, is stable in resources group, is high in natural distribution density, small in activities range, and is free of the phenomenon of long-distance migration, thereby being very suitable for the artificial cultivation. However, the current artificial culture is low in survival rate, growth speed and yield. According to the characteristics of the different growth stage of the sipunculus nudus, a proper growth cultivation environment is creased in a nichetargeting way, the pond intermediate cultivation technology of the sipunculus nudus is combined with the pond cultivation technology of the sipunculus nudus, the survival rate of the seeds is improved, the seedling quantity per unit area is increased, the artificial breeding quality is guaranteed, and the cultivating survival rate and the cultivating per unit yield are improved, so that the cultivating survival rate reaches at 85%, the cultivating per unit yield reaches at 215kg / mu, and the aim of the high-efficiency cultivation of the sipunculus nudus is realized.

The invention discloses a production method of artificial isaria cicadae miq. The production method has the advantages that the technical parameters, cultivation medium conditions and the like of the method are obtained after years of optimization, each parameter, cultivation medium proportion and production conditions are quite scientific and precise, and tests show that high-quality artificial isaria cicadae miq can be obtained by the method; the method is short in cultivation time, the cultivation medium is rice, wheat or mixture of wheat and yeast, the auxiliary materials are easy to get and low in cost, and massive industrial production can be performed; the artificial isaria cicadae miq produced by the method can be used as food, healthcare product, and medicine, or as the auxiliary materials of foods, healthcare products and medicines.

The invention provides a culture method for tiger frogs, and particularly relates to the field of aquaculture. The culture method includes the steps of frog pond construction, specifies selection and spawning, tadpole culture, froglet and adult frog feeding and disinfection management. By improving the quality of culture water and adjusting a feed formula, technical problems caused by large density, poor water quality and improper feeding in artificial culture of tiger frogs are solved, the immunity of tiger frogs is effectively improved, the morbidity of tiger frogs is reduced, and the survival rate of tiger frogs in artificial culture is increased.

The invention relates to a full-floating artificial-culture nori desiccating device and using method thereof. The full-floating artificial-culture nori desiccating device comprises four floating booms, a plurality of floating barrels, four piles and four pile cables, wherein the four floating booms are arranged in parallel, the two ends of each floating boom are connected with the piles through the pile cables, the piles are fixed in a seabed mud layer, a plurality of supporting bamboos are arranged the two middle floating booms, the floating barrels are arranged between the other two floating booms, and the floating barrels are arranged at the two ends of the supporting bamboos at the corresponding positions. When nori needs to be desiccated, the supporting bamboos are arranged on the floating barrels to achieve the desiccating purpose. By means of the full-floating artificial-culture nori desiccating device, the appropriate desiccating time is provided for the nori on a full-floating raft frame, and accordingly the effects of restraining unwanted alga, controlling diseases and rotting and the like are achieved.

The invention relates to a domesticating method for wild Spanish mackerels, which comprises the steps of the selection of the water surface for domestication, the collection of the wild Spanish mackerels, artificial domestication, and breeding selection. The method overcomes the defects that the current resources of the wild Spanish mackerels decrease gradually, and the domestication of the wild Spanish mackerels and the large-area artificial culture are not broken through. By domesticating the wild Spanish mackerels, the method can domesticate and breed the Spanish mackerel parents having theadvantages of high quality and yield, stable production, strong disease resistance as well as convenient artificial culture and transportation, and being suitable for the culture environment in China. As for the fries bred by the domesticated Spanish mackerel parents, the survival rate is increased by 15%, and the yield is increased by more than 50% so as to greatly reduce the culture cost. The method has very fundamental significance for protecting the natural resources, meeting the market requirements and increasing the economic income of fishmen.

The invention discloses a feed for portunus trituberculatus miers, belonging to the field of feed for aquatic animal culture. The feed comprises the following ingredients in percentage by weight: 35-45% of fish meal, 10-18% of soybean meal, 5-8% of spent grains liquor of corn alcohol, 3-8% of beer yeast, 3-5% of shrimp shell meal, 15-25% of high protein flour, 3-5% of squilla protein plasma, 1-3% of phospholipid oil, 1-3% of refined fish oil, 1-3% of complex binding agent, 1-2% of compound mineral substance, 0.5-1.5% of compound vitamin, 0.2-0.4% of choline and 0.1-0.4% of edible salt. Compared with the prior art, the feed provided by the invention comprises reasonable ingredients, has comprehensive nutrition, is full of nutriments, has extremely strong food calling effect on the portunus trituberculatus miers, can meet the requirements for artificial culture of the portunus trituberculatus miers in ponds, improves the food intake, effectively prevents the phenomenon that the portunus trituberculatus miers clamps the food mutually, and has obvious effect on improving the culture survival rate of the portunus trituberculatus miers.

The invention discloses a method for obtaining germination effective symbiotic fungus of Cymbidium bicolor seeds, which includes: using dead twigs, fallen leaves, bark, moss, humus and the like around an original habitant of Cymbidium bicolor as culture medium, subjecting the culture medium and the seeds of Cymbidium bicolor to ex-site symbiotic germination to induce the seeds to germinate and generate protocorms; sterilizing the surface of each obtained protocorm, culturing with thy artificial culture medium under the sterile condition, inducing endophytic fungus in the protocorms to grow, purifying the fungus to obtain a pure colony after mycelia appears; and subjecting the obtained fungus and the seeds of Cymbidium bicolor to symbiotic germination culturing, carrying out a control experiment to check the effectiveness of the obtained fungus to germination of the seeds of Cymbidium bicolor, screening germination effective symbiotic fungus for the seeds of Cymbidium bicolor, and subjecting the fungus to molecular identification and storage. The method is simple to operate, singe variety of fungi can be obtained, massive and troublesome screening is not needed, and a new way of using Cymbidium bicolor seeds and fungus to symbiotically germinate to culture seedlings is opened.

The invention discloses a device for promoting photosynthesis of photosynthetic bacteria in an artificial culture pool. The method comprises the following steps: putting at least one photosynthetic bacterium into a black mud layer of partial regions on the bottom of the artificial culture pool, conducting light energy into the black mud layer of partial regions with the photosynthetic bacteria by using an optical conducting device, and providing light energy for the photosynthetic bacteria, thereby promoting the photosynthesis and reproduction of the photosynthetic bacteria. The optical conducting part of the device is provided with an optical conductor and a light ray scattering head device, wherein the light ray scattering head device is arranged at the bottom end of the optical conductor; an air layer structure is arranged around the optical conductor and coated on the optical conductor, so that the optical conductor is isolated from the surrounding water body; and the air layer structure has air interstice into which dry air is filled, thereby forming an air layer coating on the optical conductor.

A method for artificially culturing the Hepu triploid nacre includes culturing parents, gathering spermia and ova, fertilization, inducing, collecting larvae and culturing them, breeding and culturing in sea. A method for artificial culturing pearl with said Hepu triploid nacre includes pretreating female nacre, making small mentallum sheet, inserting nuclear, recovery culture, culturing pearl and picking up. Its advantages are high radio of triploid (more than 90%), high survical rate of larvae, and high granularity and quality of pearl.

The invention discloses a wild rhinogobio ventralis continental rise pond net cage domestication method. The method includes four key technical links of the link 1, domestication pond water quality cultivation and regulation and control, specifically, applying liquid state organic fertilizer, composite probiotics and bottom material improvers at regular intervals to carry out water quality cultivation and the regulation and control, the link 2, net case establishment, and specifically a net case is mainly composed of knotless netting and a stainless steel framework and meanwhile is provided with an aeration flow generating device, the link 3, polyculture of wild rhinogobio ventralis and megalobrama pellegrini, specifically, adopting the method that artificial culture breeds are cultured in a matched mode outside the net cage to carry out domestication of wild fishes, and the link 4, ingestion domestication, specifically, adopting the method of food calling first and domestication feeding second, namely, feeding biological baits first then gradually feeding artificial mixed feed. According to the method, domestication of the wild fishes is carried out in a manner that the flow generating type net case is erected in a continental rise pond, meanwhile, the technologies of the water quality regulation and control, polyculture and live bait food calling are utilized comprehensively, and therefore the adaptability of the wild fishes to the culture environment can be promoted, the survival rate of domestication can be improved, and the method has wide application prospect.

A tissue culture technique for quickly reproducing peony seedlings includes inoculating the explant in a special culture medium, artifically regulating the action intensity of environment factor, and exponentially reproducing the seedlings in artificial culture room by the successive transfer periods (28 days). For one foliage bud, 1.65 millions seedlings can be reproduced in one year.

The invention discloses an artificial culture method of Zn-enriched cordyceps militaris mycelia and a culture medium thereof; according to weight percentage, the formulation components of the culture medium comprise: 1.0 to 3.0 percent of glucose, 0.5 to 1.0 percent of peptone, 0.1 to 1.0 percent of yeast extract, 1.0 to 2.0 percent of agar powder and the rest of water, and the pH value of the culture medium is 5.0 to 7.5. The artificial culture method comprises the steps of test tube slant surface strain culture (activation), test tube liquid strain culture, triangular flask liquid seed culture, seeding tank culture, fermenter culture, plate-frame filtration, crashing, drying in vacuum, crashing and leading the mycelia into powder. The operation is simple, the culture period is short, the yield of mycelia is high, no pollution and waste are produced, and the method has the function of supplementing zinc, thus being capable of carrying out industrial production.

An artificial culture method for north cordyceps sporophores includes such steps as selecting excellent strains (cordyceps body), separating, screening, purifying to obtain high-yield spawns, adding them to culture medium in pp bottles, culturing sporophores under light radiation and temp contron condition, inoculating into living silkworm chrysalis, and rejuvenescent culture to obtain fresh living cordyceps product. Its advantages include big body (3-4 mm X 4.5-8.5cm), higher contents of cordyceptic extract (polyose) and SOD, and low cost.

The invention discloses a solid fermentation medium for phellinus igniarius mycelium and a solid fermentation cultural method for the phellinus igniarius mycelium, belonging to the technical field of phellinus igniarius artificial culture. Corn flour, wheat bran and the like are used as materials for preparing culture medium which can be used for the solid fermentation culture medium for the phellinus igniarius mycelium. Compared with the current phellinus igniarius mycelium liquid fermentation and phellinus igniarius solid cultural method, the cultural method can acquire much phellinus igniarius mycelium while economical and convenient to operate; moreover, the content of polysaccharide and flavone is higher than that of crude polysaccharides and flavone in each gram of sporophore.

The invention provides large-scale fly larvae breeding equipment and an application method thereof. The set of large-scale fly larvae breeding equipment comprises a boiler room, a Delia platura breeding room, a young larvae breeding room and an operating platform, a fermenting tank and a manure dried yard arranged below a steel frame shed; and the application method of the set of large-scale fly larvae breeding equipment comprises the following steps of: (1) heating the Delia platura breeding room and the young larvae breeding room by using a dual-use (steam and hot water) atmospheric boiler, and maintaining the indoor temperature at 20-30 DEG C; (2) breeding of Delia platura; (3) processing of a culture medium; and (4) breeding of young larvae. The method provided by the invention uses a novel thermal insulation material to build the breeding houses and further realize heat preservation in the winter and thermal insulation in the summer with low heating cost, thus realizing ceaseless breeding of fly larvae in all the year round; the method can improve one-step manure-drying and humidifying processes, ensure constant dryness and humidity conditions of a pig manure culture medium for young larvae breeding, and ensure the breeding quality; in addition, an automatic larvae manure separation manner is used for reducing the artificial culture and improving the breeding efficiency.

The invention discloses a culturing method of artificially cicada fungus, which comprises the following steps: (1) select original bacterial strains with fast growth speed, compact and chubby bacterial colonies and obvious angle distortion and separation in later culturing phase from the paecilomyces cicada bacterial strains in the nature reserves; the bacterial strains with the typical conidial production structure when observing the angle distortion using a microscope is the paecilomyces cicada heterokaryotic bacterial strains; (2) after culturing the paecilomyces cicada of the paecilomyces cicada heterokaryotic bacterial strains in culture media for a week, use the 0.05% tweenum 80 water to separate the conidia from the paecilomyces cicada spores in the inclined plane of the test tubes or culture dishes, and then dilute the conidia into the paecilomyces cicada spore liquid with the concentration being 1*10<6>-1*10<7> conidia / ml; put the infection bodies in the paecilomyces cicada spore liquid for infection and culture for two to three weeks with normal temperature of 20 to 28 DEG C to get cicada fungus. The culturing method of artificially cicada fungus has the advantages that: the method is simple and practical; infection bodies the same as the cicada fungus collected in natural mountain forests can be gotten; the method is endowed with market popularization value.

The invention relates to an artificial culture method of cordyceps longissima sporocarp. A strain is cordyceps longissima agamotype-hirsutella longissima. The artificial culture method of the cordyceps longissima sporocarp includes A, cant tube strain activation culture; B, liquid shake flask seed culture; C, hypha stage culture; and D, sporocarp culture. The cordyceps longissima sporocarp cultured artificially has rodlike stroma and is solitary or branching and cinnamon, length is 1-7cm, the diameter is 1-3mm, a large amount of white coremium is grown at the top end or the side face of the stroma at later stage of sporocarp culture and is compact. The artificial culture method of the cordyceps longissima sporocarp is initiated, can perform large-scale culture of the cordyceps longissima sporocarp, solves the problem that wild cordyceps longissima sources are deficient, establishes a foundation for comprehensive development of cordyceps longissima edible value and industrialized production, provides technical support, and is simple in process, low in cost, wide in raw material source, non-toxic, free of pollution and capable of performing large-scale production.

An artificial culture and passage method for the Ectropis oblique hypulin W. includes such steps as after the pupa of Ectropis oblique hypulin W. becomes larva for 2 days it is buried in sandy soil, eclosion to become adult, pairing male adult with female adult, putting them in a culture container containing nutritive liquid, mating, spawning, conventional disinfecting of ova, inoculating to obtain larvae, and artificial culturing while regulating feed 1-5 times. Said feed for culturing the larvae of tea looper is proportionally prepared from wheat's germinal bud, casein, Wei's salt, sorbic acid, nipagin, VC, VB, agar, inositol and water.