25 results about "Artificial culture" patented technology

The invention discloses a method for cultivating trichogramma ostriniae in vitro, and belongs to the field of insect feeding. The artificial host ovum comprises a sealed hemisphere prepared from a plastic penetrating material, an oviposition attractant coated on the outer convex surface of the hemisphere and an internal artificial culture solution, the artificial culture solution is a tussah egg solution. According to the method, the tussah egg liquid is used for cultivating the trichogramma ostriniae, and the effect of reducing the breeding cost can be achieved. The body length of the trichogramma ostriniae cultivated by the method is increased by about 21.4% compared with that of trichogramma ostriniae bred by rice moth eggs; the average brood amount is increased by about 24.9%; the parasitic rate is obviously higher than that of trichogramma ostriniae bred by rice moth eggs. The biological control effect on the trichogramma ostriniae is remarkably improved.

The invention relates to the technical field of bioengineering, in particular to a method for promoting cordyceps sinensis to produce conidia. The invention provides a method for promoting cordyceps sinensis to produce conidia, which comprises the following steps: (1) carrying out dark culture on cordyceps sinensis on a primary solid culture medium to obtain mycelium; and (2) transferring the mycelium obtained in the step (1) to a secondary solid culture medium, and carrying out environmental stimulation culture until conidia are generated. The method is suitable for industrial production, has the advantages of simplicity and convenience in operation, controllable cost, high repeatability and the like, can effectively shorten the fermentation period, improves the inoculation efficiency and the yield of metabolites, and provides reliable technical support for large-scale artificial culture and deep development of cordyceps sinensis.

The invention provides an efficient artificial culture system and method for an irpex lacteus CZ-81 strain, the efficient artificial culture system for the irpex lacteus CZ-81 strain comprises a culture container, an environment control system, a nutrition supply system, a metabolite discharge system and a monitoring system, the culture container is of a multi-layer three-dimensional structure and comprises a plurality of independent culture units, and the culture units are connected with the environment control system and the nutrition supply system. The culture medium is used for culturing an irpex lacteus CZ-81 strain; the environment control system is used for controlling the culture temperature of the culture container through constant-temperature liquid flowing circularly; the culture humidity of the culture container is controlled in a mode of combining a humidifier and a dehumidifier; controlling the illumination of the culture container through a light source with adjustable brightness and spectrum; and controlling the gas components of the culture container through a gas mixing device. According to the technical scheme, the culture efficiency, the yield and the quality of the Irpex lacteus CZ-81 strain are improved.

The invention relates to the technical field of veterinary diagnosis in intelligent agriculture, in particular to a dairy cow mastitis pathogen rapid identification and medication guidance system, which comprises the following modules: an image acquisition module, which is configured in a microbial culture device and comprises a high-resolution image sensor and a multispectral illumination unit, the acquisition module is used for acquiring digital images of culture dishes containing pathogenic bacterium colonies and drug sensitive paper sheets; and the edge calculation module is in communication connection with the image acquisition module and is used for carrying out noise filtering and contrast enhancement processing on the acquired petri dish digital image and extracting key image area data including a bacterial colony area and a drug sensitive paper area. According to the invention, through multi-module cooperation and data closed loop, rapid identification and accurate medication of pathogenic bacteria are realized, so that the problems of treatment delay and drug resistance development caused by dependence on artificial culture and empirical judgment and long diagnosis period in traditional methods are improved.

The invention relates to the technical field of sea squirt culture, and particularly discloses a full-artificial culture system for ciona intestinalis, a seawater preparation unit is used for purifying tap water and adding sea salt to adjust the salinity of simulated seawater; the culture unit comprises a culture pond, a seawater supply system and a drainage system; the culture pond is made of a transparent material, the seawater supply system conveys prepared seawater to the culture pond through a pipeline, and the drainage system is used for regularly replacing and discharging wastewater in the culture pond; the seawater circulating unit is used for purifying seawater in the culture system and promoting circulating flow of water in the culture system; the temperature control unit is used for controlling constant water temperature in the culture pond; the illumination control unit is used for simulating natural light to irradiate the sea squirts. By means of the artificial seawater circulation system, dependence on natural seawater is completely eliminated, and full-artificial breeding of ciona ciona is achieved. According to the system, internal circulation operation is achieved, waste of water resources is reduced, and meanwhile the risk of environmental pollution is reduced.

The invention discloses a new cordyceps sinensis strain CHYOs104 and an artificial culture method thereof, and relates to the technical field of cordyceps sinensis strains and culture methods, the strain is separated from a 580m cordyceps sinensis producing area at an altitude of 4 in Nanqu county in Tibet, the strain is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is 2025-06-25, and the preservation number is CCTCC NO: M20251478. In a parallel test of three batches of 3-instar larvae of 1500 small gold hepialus armoricanus, the CHYOs104 + egg yolk culture medium enables the bombyx batryticatus rate to reach 62.2 + / -1.8% and the grass rate (the bombyx batryticatus successfully grows sporocarp of more than or equal to 2 cm) to reach 70.7 + / -2.1%, repeatability is achieved, the bombyx batryticatus rate and the grass rate are increased, and batch culture production can be achieved.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

The invention discloses an Escherichia coli strain culture method, and belongs to the field of medicine, the Escherichia coli strain culture method comprises the following steps: S1, preparing an asymmetric carbon nitrogen flow regulation type compound culture medium which comprises the following components: 10-25 g / L of a carbon source component, 4-8 g / L of a nitrogen source component, 5-10 g / L of a slow release carbon source component, 5-10 g / L of a nitrogen source component, 5-10 g / L of a nitrogen source component and the balance of water, and the mass ratio of the carbon source component to the slow release carbon source component is 3: 7; wherein the ratio of inorganic nitrogen to organic nitrogen is 1: 2, and the microelement liquid is 1-3mL / L; and S2, inoculating a seed solution containing a non-model escherichia coli strain into the culture medium, and setting initial culture parameters as follows: the temperature is 33 DEG C, the pH value is 6.8, the dissolved oxygen is maintained at 50%, and the rotating speed is 150rpm. By introducing an asymmetric carbon-nitrogen regulation strategy and a slow-release carbon source system, the metabolic environment of non-model escherichia coli is optimized, and the growth adaptability and stability of the escherichia coli in an artificial culture medium are remarkably improved.

The invention discloses a 3D skin model based on photocuring bio-printing and a construction method thereof, and the construction method comprises the following steps: A, printing bio-ink by adopting a 3D printing technology to obtain a corium layer; b, inoculating keratinocytes or epidermal stem cells to the surface of a corium layer, standing for a certain time, and then carrying out immersion culture; c, the dermis layer obtained after immersion culture is completed is added into a gas-liquid culture medium for gas-liquid culture, and the 3D skin model is obtained; wherein laminin is added into the gas-liquid culture medium in the step C. By adopting the construction scheme of combining 3D printing of the corium layer with artificial culture of the epidermal layer, the batch stability can be improved, the artificial culture period can be shortened, the cost can be reduced, and the quality of the model can be improved; the epidermal layer and the corium layer of the prepared 3D skin model are tightly connected, the epidermal layer is completely differentiated, and the 3D skin model can be widely applied to safety evaluation and efficacy evaluation of cosmetics or drugs.

The application discloses application of magnesium aluminum silicate in promoting production of blastospores of Ophiocordyceps sinensis, and is aimed at how to quickly obtain a large number of blastospores for infection in the process of artificial culture of Ophiocordyceps sinensis combined with Ophiocordyceps sinensis, wherein magnesium aluminum silicate is added in a culture medium of Ophiocordyceps sinensis to induce high-efficiency production of blastospores of Ophiocordyceps sinensis; Ophiocordyceps sinensis inoculation mother liquor is inoculated in the culture medium with 1.0-5.0 g / L magnesium aluminum silicate; and the spore production amount of the culture medium with added magnesium aluminum silicate is increased by 4-14 times than that of the culture medium without added magnesium aluminum silicate within a blastospore collection time (25-45 days); the most suitable addition amount is between 2.0-3.5 g / L, the spore production amount is increased by about 10 times than that of the culture medium without added magnesium aluminum silicate on average; the addition of magnesium aluminum silicate can also increase the tolerance and the living ability of blastospores, and can prolong the collection time by 45-60 days. The application can more efficiently obtain blastospores, and can shorten the artificial culture time of Ophiocordyceps sinensis and save the artificial culture cost.

The invention provides a basil hairy root extract and a preparation method and application thereof, the basil hairy root extract at least contains polyphenol and flavone, the polyphenol content is not less than 35 mg / g, and the flavone content is not less than 120 mg / g. The preparation method comprises the following steps: infecting basil callus cells with agrobacterium tumefaciens, and culturing hairy roots through an artificial culture medium; and drying the obtained basil hairy roots, and adding a solvent to extract active ingredients, thereby obtaining the basil hairy root extract. According to the method, the sweet basil callus cells are infected by agrobacterium tumefaciens, and the hairy roots are cultured by an artificial culture medium, so that efficient culture of the sweet basil hairy roots is realized, and theoretical support and technical guarantee are provided for deep development of sweet basil resources and development of novel health-care and skin-care products.

The application discloses an indoor algae-be symbiotic artificial culture system and method, and belongs to the field of artificial culture of algae-be symbiotic system. The system comprises a culture box with a box cover, an attachment substrate device and a water environment control system. A partition plate is vertically arranged in the culture box to form a water flow channel between the partition plate and the inner wall of the culture box, and two functional areas, i.e. a first area and a second area, are formed on the two sides of the partition plate. The attachment substrate device is arranged in the second area. The water environment control system comprises a flow rate regulating unit, a light unit, a heating unit, a dissolved oxygen unit and a control unit. The flow rate regulating unit, the light unit, the heating unit and the dissolved oxygen unit are arranged in the first area and are controlled by the control unit. The method comprises the following steps: adding shellfish to the attachment substrate device; adding algae to the second area; and regulating the water flow rate, the light intensity, the water temperature and the water dissolved oxygen by the water environment control system. The application is applied to the indoor artificial breeding of freshwater zebra mussels and can improve the survival rate of the artificial breeding of the freshwater zebra mussels.

The invention relates to the technical field of microorganisms, in particular to a preparation method and application of a fungus with a growth promoting function and an extract of the fungus, the classification name of the fungus is Fusarium Fraxinea (PK01), the preservation mechanism of the fungus is China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.42053, and the preservation date is July 9, 2025. Macrofungus sporocarp collected from Changbai Mountain wild is separated to obtain the fungus with a growth promoting function, the hypha extract under artificial culture conditions, and active ingredients of the hypha extract for promoting growth of main crops such as corn, improving drought tolerance and waterlogging tolerance, promoting growth of rhizosphere microorganisms, promoting flowering and improving yield.

The invention discloses application of cationic polymer compounded bentonite flocculation for harvesting wide-salinity microalgae and a harvesting method, and belongs to the technical field of environmental protection. The method comprises the following steps: adding a polydimethyldiallylammonium chloride solution and a sodium bentonite suspension into an algae solution according to the cell biomass of microalgae to be harvested, stirring, settling and collecting to finish harvesting. According to the method disclosed by the invention, the microalgae in the wide-salt environment are harvested by combining PDDAC and BE for flocculation, the microalgae can be efficiently flocculated and harvested within the range of 0-40% of water salinity, and the harvesting rate can reach 90% or above; the flocculating efficiency is high, the dosage of a flocculating agent is small, the floc is large in size and compact, and the salt-resistant characteristic is remarkable; the method is suitable for efficient harvesting of microalgae in artificial culture or natural water under a wide salinity condition, provides a key technology for the field of microalgae harvesting, and has a wide application prospect; the problems of poor salinity tolerance of a flocculating agent, large dosage of the flocculating agent and poor harvesting effect in microalgae harvesting through flocculation at present are solved.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

This invention relates to a rooting culture medium and tissue culture method for Rhizoma Cypripedium, belonging to the field of plant culture technology. The tissue culture method includes the following steps: obtaining explants, sterilizing them, transplanting them to a seedling culture medium for primary culture, subculturing, and then transplanting the subcultured explants to a rooting culture medium for rooting culture to obtain Rhizoma Cypripedium seedlings; the seedling culture medium and rooting culture medium are sugar-free media. This method enables the artificial culture of Rhizoma Cypripedium, with high rooting rate, high seedling establishment rate, and high survival rate of transplanted Rhizoma Cypripedium seedlings.

The invention relates to the technical field of biology, and discloses a bifunctional bacillus strain YJ211 and application thereof, the bifunctional bacillus strain YJ211 is bacillus sp. YJ211, the preservation number of the bifunctional bacillus strain YJ211 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M20251511, and the preservation date is July 2, 2025. The bacillus sp. YJ211 strain provided by the invention can be smoothly cultured on an artificial culture medium and is easy to obtain and breed, which is crucial for being used as a strain for degrading microplastics and preventing and treating cotton verticillium wilt, and is convenient to apply and popularize in industrial production. The YJ211 strain shows remarkable degradation capacity on PET (polyethylene terephthalate) micro-plastics, and the degradation efficiency of the YJ211 strain can reach 32.71%.

The present application belongs to the technical field of fish culture, and provides a large-specification Esxoleterus notatus fry culture method. The culture method comprises the following steps: pond pretreatment, fry release, feed feeding, water quality management, and disease prevention and treatment. The culture method is reasonable and simple, overcomes the low survival rate of artificial culture of Esxoleterus notatus in the prior art, is suitable for industrialized production, and has important significance for improving the comprehensive culture benefit of Esxoleterus notatus.

The invention provides a Fuscoporia lilacinus strain as well as a culture method and application thereof, and relates to the technical field of microorganisms. According to the method, biological characteristics and culture conditions of the Fuscoporia lilacinus are defined, the technical problem of artificial culture of the Fuscoporia lilacinus is solved, artificial culture of the Fuscoporia lilacinus is realized for the first time, and a new variety and a new culture way are provided for new resource utilization of rare edible and medicinal fungi in China. The invention provides a Fuscoporia lilacinus strain culture formula and a solid-liquid coupled liquid strain culture method for strain propagation, and mycelia grow fast and are strong in vitality. According to the invention, functional evaluation is carried out on the active ingredients of the sporocarp of the Fuscoporia lilacinus, the extract with the blood sugar reducing activity effect is obtained, and a new raw material source is provided for research and development of medicines and health-care products.

Provided herein are artificially cultured somites comprising mature muscle progenitor cells and / or mature muscle cells, methods of obtaining same and methods for fast, large scale production of cultured meat comprising mature muscle cells.

The utility model belongs to the technical field of ganoderma lucidum planting, and particularly relates to an artificial cultivation frame for ganoderma lucidum planting, which comprises a frame body, a support column is fixedly connected in the frame body, placing frames are fixedly connected between the two sides of the outer part of the support column and the inner wall of the frame body at equal intervals, and limiting plates are symmetrically and fixedly connected on the two sides of the outer part of the support column at equal intervals; the utility model provides an artificial cultivation frame for ganoderma lucidum planting, and solves the problems that in the prior art, when ganoderma lucidum is cultivated, a humidifier is generally installed in a cultivation room for humidity control, the growth environment of the ganoderma lucidum is guaranteed, but the humidifier cannot directly spray water mist to the cultivation frame, the spraying uniformity of the ganoderma lucidum is reduced, and the growth environment of the ganoderma lucidum is affected. The problems that the growth quality of ganoderma lucidum is reduced due to the fact that indoor humidity rises for a long time, and meanwhile water resources are wasted due to the fact that spraying needs to be conducted on the whole room, the ventilation effect is common, and the optimal growth environment cannot be guaranteed are solved.

Provided herein are artificially cultured somites comprising mature muscle progenitor cells and / or mature muscle cells, methods of obtaining same and methods for fast, large scale production of cultured meat comprising mature muscle cells.

The invention provides a method for obtaining a large number of metarhizium anisopliae conidia, and belongs to the technical field of microorganisms. The method comprises the following steps: inoculating 4-instar living larvae of spodoptera frugiperda noctuid with metarhizium anisopliae spore powder to cause infection, carrying out amplification culture on the infected spodoptera frugiperda noctuid, and collecting spores in the 4th day after the spodoptera frugiperda body is dead, so as to obtain a large amount of metarhizium anisopliae conidia. According to the method provided by the invention, the operation process is greatly simplified, the spore production time is shortened, the spore yield is improved, the production cost is reduced, and meanwhile, the problem of strain degeneration caused by continuous culture of metarhizium ledebusii on an artificial culture medium is solved.

The invention provides an artificial culture method of stiff pupae, and belongs to the technical field of traditional Chinese medicinal material culture. The invention provides an artificial culture method of stiff pupae, which comprises the following steps: spraying a beauveria bassiana solution on the body surface of a silkworm body, and culturing the silkworm body to obtain the stiff pupae. According to the method, the beauveria bassiana liquid is sprayed on the surfaces of silkworm bodies for inoculation, the death rate in the silkworm chrysalis stiffening process can be remarkably reduced, the culture yield of stiff pupae is increased, and then the yield of the stiff pupae is increased. Furthermore, a matured silkworm precursor spray inoculation method is adopted, and compared with a live pupa puncture inoculation method, the matured silkworm precursor spray inoculation method has the advantages that the death rate in the silkworm pupa stiffening process can be more remarkably reduced, the culture yield of stiff pupae is increased, and the stiff pupa yield is increased.