12 results about "Artificial culture" patented technology

The invention relates to the technical field of bioengineering, in particular to a method for promoting cordyceps sinensis to produce conidia. The invention provides a method for promoting cordyceps sinensis to produce conidia, which comprises the following steps: (1) carrying out dark culture on cordyceps sinensis on a primary solid culture medium to obtain mycelium; and (2) transferring the mycelium obtained in the step (1) to a secondary solid culture medium, and carrying out environmental stimulation culture until conidia are generated. The method is suitable for industrial production, has the advantages of simplicity and convenience in operation, controllable cost, high repeatability and the like, can effectively shorten the fermentation period, improves the inoculation efficiency and the yield of metabolites, and provides reliable technical support for large-scale artificial culture and deep development of cordyceps sinensis.

The invention relates to the technical field of veterinary diagnosis in intelligent agriculture, in particular to a dairy cow mastitis pathogen rapid identification and medication guidance system, which comprises the following modules: an image acquisition module, which is configured in a microbial culture device and comprises a high-resolution image sensor and a multispectral illumination unit, the acquisition module is used for acquiring digital images of culture dishes containing pathogenic bacterium colonies and drug sensitive paper sheets; and the edge calculation module is in communication connection with the image acquisition module and is used for carrying out noise filtering and contrast enhancement processing on the acquired petri dish digital image and extracting key image area data including a bacterial colony area and a drug sensitive paper area. According to the invention, through multi-module cooperation and data closed loop, rapid identification and accurate medication of pathogenic bacteria are realized, so that the problems of treatment delay and drug resistance development caused by dependence on artificial culture and empirical judgment and long diagnosis period in traditional methods are improved.

The invention discloses a new cordyceps sinensis strain CHYOs104 and an artificial culture method thereof, and relates to the technical field of cordyceps sinensis strains and culture methods, the strain is separated from a 580m cordyceps sinensis producing area at an altitude of 4 in Nanqu county in Tibet, the strain is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is 2025-06-25, and the preservation number is CCTCC NO: M20251478. In a parallel test of three batches of 3-instar larvae of 1500 small gold hepialus armoricanus, the CHYOs104 + egg yolk culture medium enables the bombyx batryticatus rate to reach 62.2 + / -1.8% and the grass rate (the bombyx batryticatus successfully grows sporocarp of more than or equal to 2 cm) to reach 70.7 + / -2.1%, repeatability is achieved, the bombyx batryticatus rate and the grass rate are increased, and batch culture production can be achieved.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

The application discloses an indoor algae-be symbiotic artificial culture system and method, and belongs to the field of artificial culture of algae-be symbiotic system. The system comprises a culture box with a box cover, an attachment substrate device and a water environment control system. A partition plate is vertically arranged in the culture box to form a water flow channel between the partition plate and the inner wall of the culture box, and two functional areas, i.e. a first area and a second area, are formed on the two sides of the partition plate. The attachment substrate device is arranged in the second area. The water environment control system comprises a flow rate regulating unit, a light unit, a heating unit, a dissolved oxygen unit and a control unit. The flow rate regulating unit, the light unit, the heating unit and the dissolved oxygen unit are arranged in the first area and are controlled by the control unit. The method comprises the following steps: adding shellfish to the attachment substrate device; adding algae to the second area; and regulating the water flow rate, the light intensity, the water temperature and the water dissolved oxygen by the water environment control system. The application is applied to the indoor artificial breeding of freshwater zebra mussels and can improve the survival rate of the artificial breeding of the freshwater zebra mussels.

The invention relates to the technical field of microorganisms, in particular to a preparation method and application of a fungus with a growth promoting function and an extract of the fungus, the classification name of the fungus is Fusarium Fraxinea (PK01), the preservation mechanism of the fungus is China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.42053, and the preservation date is July 9, 2025. Macrofungus sporocarp collected from Changbai Mountain wild is separated to obtain the fungus with a growth promoting function, the hypha extract under artificial culture conditions, and active ingredients of the hypha extract for promoting growth of main crops such as corn, improving drought tolerance and waterlogging tolerance, promoting growth of rhizosphere microorganisms, promoting flowering and improving yield.

This invention relates to a rooting culture medium and tissue culture method for Rhizoma Cypripedium, belonging to the field of plant culture technology. The tissue culture method includes the following steps: obtaining explants, sterilizing them, transplanting them to a seedling culture medium for primary culture, subculturing, and then transplanting the subcultured explants to a rooting culture medium for rooting culture to obtain Rhizoma Cypripedium seedlings; the seedling culture medium and rooting culture medium are sugar-free media. This method enables the artificial culture of Rhizoma Cypripedium, with high rooting rate, high seedling establishment rate, and high survival rate of transplanted Rhizoma Cypripedium seedlings.

The invention relates to the technical field of biology, and discloses a bifunctional bacillus strain YJ211 and application thereof, the bifunctional bacillus strain YJ211 is bacillus sp. YJ211, the preservation number of the bifunctional bacillus strain YJ211 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M20251511, and the preservation date is July 2, 2025. The bacillus sp. YJ211 strain provided by the invention can be smoothly cultured on an artificial culture medium and is easy to obtain and breed, which is crucial for being used as a strain for degrading microplastics and preventing and treating cotton verticillium wilt, and is convenient to apply and popularize in industrial production. The YJ211 strain shows remarkable degradation capacity on PET (polyethylene terephthalate) micro-plastics, and the degradation efficiency of the YJ211 strain can reach 32.71%.

The present application belongs to the technical field of fish culture, and provides a large-specification Esxoleterus notatus fry culture method. The culture method comprises the following steps: pond pretreatment, fry release, feed feeding, water quality management, and disease prevention and treatment. The culture method is reasonable and simple, overcomes the low survival rate of artificial culture of Esxoleterus notatus in the prior art, is suitable for industrialized production, and has important significance for improving the comprehensive culture benefit of Esxoleterus notatus.

The invention provides a Fuscoporia lilacinus strain as well as a culture method and application thereof, and relates to the technical field of microorganisms. According to the method, biological characteristics and culture conditions of the Fuscoporia lilacinus are defined, the technical problem of artificial culture of the Fuscoporia lilacinus is solved, artificial culture of the Fuscoporia lilacinus is realized for the first time, and a new variety and a new culture way are provided for new resource utilization of rare edible and medicinal fungi in China. The invention provides a Fuscoporia lilacinus strain culture formula and a solid-liquid coupled liquid strain culture method for strain propagation, and mycelia grow fast and are strong in vitality. According to the invention, functional evaluation is carried out on the active ingredients of the sporocarp of the Fuscoporia lilacinus, the extract with the blood sugar reducing activity effect is obtained, and a new raw material source is provided for research and development of medicines and health-care products.

Provided herein are artificially cultured somites comprising mature muscle progenitor cells and / or mature muscle cells, methods of obtaining same and methods for fast, large scale production of cultured meat comprising mature muscle cells.

The invention provides an artificial culture method of stiff pupae, and belongs to the technical field of traditional Chinese medicinal material culture. The invention provides an artificial culture method of stiff pupae, which comprises the following steps: spraying a beauveria bassiana solution on the body surface of a silkworm body, and culturing the silkworm body to obtain the stiff pupae. According to the method, the beauveria bassiana liquid is sprayed on the surfaces of silkworm bodies for inoculation, the death rate in the silkworm chrysalis stiffening process can be remarkably reduced, the culture yield of stiff pupae is increased, and then the yield of the stiff pupae is increased. Furthermore, a matured silkworm precursor spray inoculation method is adopted, and compared with a live pupa puncture inoculation method, the matured silkworm precursor spray inoculation method has the advantages that the death rate in the silkworm pupa stiffening process can be more remarkably reduced, the culture yield of stiff pupae is increased, and the stiff pupa yield is increased.