40 results about "Artificial culture" patented technology

The invention discloses a cordyceps sinensis factory culture method which comprises the following steps: inoculating conidia of cordyceps sinensis into hepialus armoricanus larvae, and culturing bacteria-sensitive larvae; collecting an appearance image of the cordyceps sinensis, and judging the growth stage of the cordyceps sinensis according to the collected image; according to the real-time cordyceps sinensis growth stage, a corresponding environment instruction is called to control the environment parameters of the multi-layer culture frame area. According to the method, after the conidia are inoculated into the hepialus armoricanus larvae, artificial culture is carried out, the growth stages of cordyceps sinensis are judged through image acquisition, different illumination control conditions are preset for different growth stages, self-adaptive illumination control is achieved, optimal environment control is provided for growth of cordyceps sinensis, and the method is suitable for large-scale popularization and application. The grown cordyceps sinensis is good in form and rich in nutrient substances.

The invention provides an artificial culture method of phellinus igniarius and a preparation method and application of a phellinus igniarius extract, and belongs to the technical field of fungus extracts. The invention provides an artificial culture method of phellinus igniarius, which comprises the following steps: carrying out shake culture on the phellinus igniarius by using a liquid culture medium, and preparing a phellinus igniarius extract after the phellinus igniarius is cultured for 14 days. According to the invention, artificially cultured phellinus igniarius is adopted as a raw material for preparation, compared with wild phellinus igniarius, the production cost is remarkably reduced, the phellinus igniarius extract obtained after extraction contains a plurality of active components, and compared with the previous research on a single component, the mixed components can play a better liver protection characteristic, and the liver protection effect is better. The protection effect on hepatocytes is realized through the anti-oxidation performance. Meanwhile, the phellinus igniarius extract prepared by the invention can also be used as a regulating protective agent for oxidative stress, inflammatory response, cell apoptosis and mitochondrial dysfunction, exerts the protection characteristic of liver cells, and lays a foundation for development of phellinus igniarius related liver protection products.

The invention discloses a method for cultivating trichogramma ostriniae in vitro, and belongs to the field of insect feeding. The artificial host ovum comprises a sealed hemisphere prepared from a plastic penetrating material, an oviposition attractant coated on the outer convex surface of the hemisphere and an internal artificial culture solution, the artificial culture solution is a tussah egg solution. According to the method, the tussah egg liquid is used for cultivating the trichogramma ostriniae, and the effect of reducing the breeding cost can be achieved. The body length of the trichogramma ostriniae cultivated by the method is increased by about 21.4% compared with that of trichogramma ostriniae bred by rice moth eggs; the average brood amount is increased by about 24.9%; the parasitic rate is obviously higher than that of trichogramma ostriniae bred by rice moth eggs. The biological control effect on the trichogramma ostriniae is remarkably improved.

The invention relates to the technical field of bioengineering, in particular to a method for promoting cordyceps sinensis to produce conidia. The invention provides a method for promoting cordyceps sinensis to produce conidia, which comprises the following steps: (1) carrying out dark culture on cordyceps sinensis on a primary solid culture medium to obtain mycelium; and (2) transferring the mycelium obtained in the step (1) to a secondary solid culture medium, and carrying out environmental stimulation culture until conidia are generated. The method is suitable for industrial production, has the advantages of simplicity and convenience in operation, controllable cost, high repeatability and the like, can effectively shorten the fermentation period, improves the inoculation efficiency and the yield of metabolites, and provides reliable technical support for large-scale artificial culture and deep development of cordyceps sinensis.

The invention provides an efficient artificial culture system and method for an irpex lacteus CZ-81 strain, the efficient artificial culture system for the irpex lacteus CZ-81 strain comprises a culture container, an environment control system, a nutrition supply system, a metabolite discharge system and a monitoring system, the culture container is of a multi-layer three-dimensional structure and comprises a plurality of independent culture units, and the culture units are connected with the environment control system and the nutrition supply system. The culture medium is used for culturing an irpex lacteus CZ-81 strain; the environment control system is used for controlling the culture temperature of the culture container through constant-temperature liquid flowing circularly; the culture humidity of the culture container is controlled in a mode of combining a humidifier and a dehumidifier; controlling the illumination of the culture container through a light source with adjustable brightness and spectrum; and controlling the gas components of the culture container through a gas mixing device. According to the technical scheme, the culture efficiency, the yield and the quality of the Irpex lacteus CZ-81 strain are improved.

An artificial culture medium for mushroom cultivation, which contains amorphous calcium aluminosilicate that contains CaO in an amount of 30 to 60% by mass, Al2O3 in an amount of 10 to 60% by mass, SiO2 in an amount of 5 to 40% by mass, and P2O5 in an amount of 100 to 5,000 ppm by mass.

The present invention discloses a method for ecological restoration of a sphagnum swamp wetland, comprising the following steps: collecting sphagnum swamp wetland plants and peat soil in the habitat to be restored, moving them to a greenhouse for cultivation, and increasing the number of sphagnum swamp wetland plants; removing invasive plant plants and propagules; cutting off the root zone of the invasive plant and the soil above and below it to construct a restoration groove; keeping the restoration groove in a flooded state to be restored; laying a matrix layer on the restoration groove, and covering the matrix layer with the sphagnum swamp wetland cultivated by the present invention. The present invention rapidly increases the number of sphagnum swamp wetland ecological restoration plants by artificially cultivating the sphagnum swamp growing in the restoration site. In addition, the present invention selects the sphagnum swamp wetland plants and peat soil to be restored, thereby avoiding the ecological differences between the restored and transplanted sphagnum swamp wetland and the sphagnum swamp wetland, and the sphagnum swamp wetland plants used for ecological restoration can adapt to the ecological environment of the restoration site more quickly, with a higher survival rate and a good restoration effect.

The invention relates to the technical field of veterinary diagnosis in intelligent agriculture, in particular to a dairy cow mastitis pathogen rapid identification and medication guidance system, which comprises the following modules: an image acquisition module, which is configured in a microbial culture device and comprises a high-resolution image sensor and a multispectral illumination unit, the acquisition module is used for acquiring digital images of culture dishes containing pathogenic bacterium colonies and drug sensitive paper sheets; and the edge calculation module is in communication connection with the image acquisition module and is used for carrying out noise filtering and contrast enhancement processing on the acquired petri dish digital image and extracting key image area data including a bacterial colony area and a drug sensitive paper area. According to the invention, through multi-module cooperation and data closed loop, rapid identification and accurate medication of pathogenic bacteria are realized, so that the problems of treatment delay and drug resistance development caused by dependence on artificial culture and empirical judgment and long diagnosis period in traditional methods are improved.

The invention belongs to the technical field of large-scale shellfish offspring seed cultivation, and discloses a method for artificial domestication, ripening promotion, spawning induction and egg bag collection of festival snails, the optimal food and bait combination of the festival snails is successfully found out through experiments, sufficient algae bait is cultured by adopting an artificial culture mode, the natural living environment of the festival snails is simulated, and the festival snails can be bred in a large scale. The artificial domestication of the snails is successfully completed. The method is similar to other shellfish ripening, and the purpose of ripening can be achieved by increasing the bait amount and enhancing the nutrition of parent snails. According to the method, the problem of spawning induction of the snails is efficiently solved through a simulated ecological breeding combination method of reducing salinity, providing attachments such as fine sand and the like. By means of the method, artificial domestication of the hedge snails is achieved for the first time in the world, the hedge snails completely adapt to the artificial breeding environment, artificial maturity promoting and spawning inducing work is completed for the first time, the egg laying amount is large, the quality is high, and solid foundation support is provided for subsequent artificial breeding of the hedge snails.

The invention relates to the technical field of sea squirt culture, and particularly discloses a full-artificial culture system for ciona intestinalis, a seawater preparation unit is used for purifying tap water and adding sea salt to adjust the salinity of simulated seawater; the culture unit comprises a culture pond, a seawater supply system and a drainage system; the culture pond is made of a transparent material, the seawater supply system conveys prepared seawater to the culture pond through a pipeline, and the drainage system is used for regularly replacing and discharging wastewater in the culture pond; the seawater circulating unit is used for purifying seawater in the culture system and promoting circulating flow of water in the culture system; the temperature control unit is used for controlling constant water temperature in the culture pond; the illumination control unit is used for simulating natural light to irradiate the sea squirts. By means of the artificial seawater circulation system, dependence on natural seawater is completely eliminated, and full-artificial breeding of ciona ciona is achieved. According to the system, internal circulation operation is achieved, waste of water resources is reduced, and meanwhile the risk of environmental pollution is reduced.

The invention discloses a low-energy-consumption microalgae cell separating and harvesting device and method based on plant fibers, and particularly relates to the field of microalgae cell concentration and separation. Comprising a separation box, the separation box communicates with a liquid inlet pipe and an air inlet pipe, the liquid inlet pipe is provided with a first valve, the air inlet pipe is connected with an inflation pump, the separation box communicates with a liquid discharging pipe, the liquid discharging pipe is provided with a second valve, the two sides of the separation box communicate with feeding channels, and the space between the two feeding channels is filled with a plant fiber filtering layer. A fixed plate and a movable plate are arranged in the separation box, a first water passing hole is formed in the fixed plate, a driving assembly used for driving the movable plate to slide in the separation box is arranged on the movable plate, and a second water passing hole is formed in the movable plate. By the adoption of the technical scheme, the problem that an existing device cannot achieve efficient collection and separation of a large number of algae cells in an artificial culture scene or an algae bloom scene of unicellular algae is solved, and resource utilization of natural plant fiber materials and efficient harvesting of the algae cells are achieved.

The invention discloses a new cordyceps sinensis strain CHYOs104 and an artificial culture method thereof, and relates to the technical field of cordyceps sinensis strains and culture methods, the strain is separated from a 580m cordyceps sinensis producing area at an altitude of 4 in Nanqu county in Tibet, the strain is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is 2025-06-25, and the preservation number is CCTCC NO: M20251478. In a parallel test of three batches of 3-instar larvae of 1500 small gold hepialus armoricanus, the CHYOs104 + egg yolk culture medium enables the bombyx batryticatus rate to reach 62.2 + / -1.8% and the grass rate (the bombyx batryticatus successfully grows sporocarp of more than or equal to 2 cm) to reach 70.7 + / -2.1%, repeatability is achieved, the bombyx batryticatus rate and the grass rate are increased, and batch culture production can be achieved.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

The invention discloses an Escherichia coli strain culture method, and belongs to the field of medicine, the Escherichia coli strain culture method comprises the following steps: S1, preparing an asymmetric carbon nitrogen flow regulation type compound culture medium which comprises the following components: 10-25 g / L of a carbon source component, 4-8 g / L of a nitrogen source component, 5-10 g / L of a slow release carbon source component, 5-10 g / L of a nitrogen source component, 5-10 g / L of a nitrogen source component and the balance of water, and the mass ratio of the carbon source component to the slow release carbon source component is 3: 7; wherein the ratio of inorganic nitrogen to organic nitrogen is 1: 2, and the microelement liquid is 1-3mL / L; and S2, inoculating a seed solution containing a non-model escherichia coli strain into the culture medium, and setting initial culture parameters as follows: the temperature is 33 DEG C, the pH value is 6.8, the dissolved oxygen is maintained at 50%, and the rotating speed is 150rpm. By introducing an asymmetric carbon-nitrogen regulation strategy and a slow-release carbon source system, the metabolic environment of non-model escherichia coli is optimized, and the growth adaptability and stability of the escherichia coli in an artificial culture medium are remarkably improved.

The invention discloses a 3D skin model based on photocuring bio-printing and a construction method thereof, and the construction method comprises the following steps: A, printing bio-ink by adopting a 3D printing technology to obtain a corium layer; b, inoculating keratinocytes or epidermal stem cells to the surface of a corium layer, standing for a certain time, and then carrying out immersion culture; c, the dermis layer obtained after immersion culture is completed is added into a gas-liquid culture medium for gas-liquid culture, and the 3D skin model is obtained; wherein laminin is added into the gas-liquid culture medium in the step C. By adopting the construction scheme of combining 3D printing of the corium layer with artificial culture of the epidermal layer, the batch stability can be improved, the artificial culture period can be shortened, the cost can be reduced, and the quality of the model can be improved; the epidermal layer and the corium layer of the prepared 3D skin model are tightly connected, the epidermal layer is completely differentiated, and the 3D skin model can be widely applied to safety evaluation and efficacy evaluation of cosmetics or drugs.

The application discloses application of magnesium aluminum silicate in promoting production of blastospores of Ophiocordyceps sinensis, and is aimed at how to quickly obtain a large number of blastospores for infection in the process of artificial culture of Ophiocordyceps sinensis combined with Ophiocordyceps sinensis, wherein magnesium aluminum silicate is added in a culture medium of Ophiocordyceps sinensis to induce high-efficiency production of blastospores of Ophiocordyceps sinensis; Ophiocordyceps sinensis inoculation mother liquor is inoculated in the culture medium with 1.0-5.0 g / L magnesium aluminum silicate; and the spore production amount of the culture medium with added magnesium aluminum silicate is increased by 4-14 times than that of the culture medium without added magnesium aluminum silicate within a blastospore collection time (25-45 days); the most suitable addition amount is between 2.0-3.5 g / L, the spore production amount is increased by about 10 times than that of the culture medium without added magnesium aluminum silicate on average; the addition of magnesium aluminum silicate can also increase the tolerance and the living ability of blastospores, and can prolong the collection time by 45-60 days. The application can more efficiently obtain blastospores, and can shorten the artificial culture time of Ophiocordyceps sinensis and save the artificial culture cost.

The experimental animal survival condition control and excrement automatic collection instrument comprises a bottom plate, a first motor is fixedly installed on one side of the bottom plate, a first threaded rod is fixedly installed at the output end of the first motor, and a first sliding table is installed on the front surface of the first threaded rod in a threaded mode; and a second motor is fixedly installed at the top of the first sliding table through a support, a second threaded rod is fixedly installed at the output end of the second motor, and a third sliding table is installed on the front surface of the second threaded rod in a threaded mode. According to the technical scheme, the problems that at present, experimental animals are mainly bred in an artificial culture mode, animal waste is manually collected to collect intestinal flora, the workload is large, animal survival conditions are inaccurate to control and the like, and the problem that a large number of flora dies due to the fact that waste is not collected in time easily occurs are solved; the device solves the problems, and achieves the advantages of automatic collection and improvement of the working efficiency.

The invention discloses an indoor algal-shellfish symbiosis artificial culture system and method. The system comprises a culture box with a box cover, an adhesion matrix device and a water environment control system, a partition plate is vertically arranged in the incubator, so that a water flow channel is formed between the partition plate and the inner wall of the incubator, and two functional areas, namely a first area and a second area, are further formed on the two sides of the partition plate; the substrate attaching device is arranged in the second area; the water environment control system comprises a flow speed regulation and control unit, an illumination unit, a heating unit, an oxygen dissolving unit and a control unit, and the flow speed regulation and control unit, the illumination unit, the heating unit and the oxygen dissolving unit are all distributed in the first area and controlled by the control unit. The method comprises the following steps: adding shellfish onto an adhesion substrate device; adding algae into the second area; the water environment control system is used for regulating and controlling the water body flow velocity, illumination intensity, water body temperature and water body dissolved oxygen. The method is applied to indoor artificial propagation of the limnoperna fortunei, and the artificial breeding survival rate of the limnoperna fortunei can be increased.

The present invention provides a culture medium for in vitro culturing Exorista sorbillans Wiedemann and a culturing method thereof. The culture medium includes a liquid culture medium and sterile absorbent cotton. The liquid culture medium is a mixed solution of skim milk, sucrose, yeast extract, egg yolk, silkworm hemolymph, and gentamicin sulfate. The culturing method of the present invention mainly includes attracting Exorista sorbillans Wiedemann to lay eggs with live insects at a certain temperature, and culturing Exorista sorbillans Wiedemann larvae in vitro with an optimized artificial culture medium. The present invention uses live insects to collect a large number of Exorista sorbillans Wiedemann eggs, overcoming the problem of difficult acquisition of fly eggs under the condition of no host. At the same time, adding silkworm hemolymph at a specific period to the in vitro culture medium significantly reduces the development time of maggots and improves the adult fly rate of Exorista sorbillans Wiedemann. The parasitic effect of the in vitro cultured tachinid fly obtained by the present invention has no obvious difference from that of the adult fly parasitized in the host. The above materials are widely available, with low costs, and the operation is simple and feasible, and the whole-year culturing and subculture of Exorista sorbillans Wiedemann can be completely realized.

The invention provides a basil hairy root extract and a preparation method and application thereof, the basil hairy root extract at least contains polyphenol and flavone, the polyphenol content is not less than 35 mg / g, and the flavone content is not less than 120 mg / g. The preparation method comprises the following steps: infecting basil callus cells with agrobacterium tumefaciens, and culturing hairy roots through an artificial culture medium; and drying the obtained basil hairy roots, and adding a solvent to extract active ingredients, thereby obtaining the basil hairy root extract. According to the method, the sweet basil callus cells are infected by agrobacterium tumefaciens, and the hairy roots are cultured by an artificial culture medium, so that efficient culture of the sweet basil hairy roots is realized, and theoretical support and technical guarantee are provided for deep development of sweet basil resources and development of novel health-care and skin-care products.

The application discloses an indoor algae-be symbiotic artificial culture system and method, and belongs to the field of artificial culture of algae-be symbiotic system. The system comprises a culture box with a box cover, an attachment substrate device and a water environment control system. A partition plate is vertically arranged in the culture box to form a water flow channel between the partition plate and the inner wall of the culture box, and two functional areas, i.e. a first area and a second area, are formed on the two sides of the partition plate. The attachment substrate device is arranged in the second area. The water environment control system comprises a flow rate regulating unit, a light unit, a heating unit, a dissolved oxygen unit and a control unit. The flow rate regulating unit, the light unit, the heating unit and the dissolved oxygen unit are arranged in the first area and are controlled by the control unit. The method comprises the following steps: adding shellfish to the attachment substrate device; adding algae to the second area; and regulating the water flow rate, the light intensity, the water temperature and the water dissolved oxygen by the water environment control system. The application is applied to the indoor artificial breeding of freshwater zebra mussels and can improve the survival rate of the artificial breeding of the freshwater zebra mussels.

The invention relates to the technical field of microorganisms, in particular to a preparation method and application of a fungus with a growth promoting function and an extract of the fungus, the classification name of the fungus is Fusarium Fraxinea (PK01), the preservation mechanism of the fungus is China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.42053, and the preservation date is July 9, 2025. Macrofungus sporocarp collected from Changbai Mountain wild is separated to obtain the fungus with a growth promoting function, the hypha extract under artificial culture conditions, and active ingredients of the hypha extract for promoting growth of main crops such as corn, improving drought tolerance and waterlogging tolerance, promoting growth of rhizosphere microorganisms, promoting flowering and improving yield.

The invention relates to the technical field of microorganisms, and discloses acinetobacter W107 and application thereof in degradation of polyethylene glycol terephthalate microplastics, the bacteria are acinetobacter soli W107 bacteria, and the preservation number of the acinetobacter soli W107 bacteria in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M20242613. The Acinetobacter soli W107 strain disclosed by the invention can be smoothly cultured on an artificial culture medium, is easy to obtain and breed, and shows remarkable degradation capacity on PET (Polyethylene Terephthalate) micro-plastics, and the degradation efficiency of the Acinetobacter soli W107 strain can reach 11.4 percent.

To provide an artificial cultured skin with excellent skin tissue (for example, properly formed horny layers and appropriately expressed genes).SOLUTION: An artificial cultured skin comprises: a basal tissue layer, comprising adipose-derived stem cells and a gel; a dermal tissue layer, disposed on the basal tissue layer and comprising fibroblasts and a gel; and an epidermal layer, disposed on the dermal tissue layer and comprising epidermal cells. Preferably, the gel is a collagen gel or a fibrin gel. Preferably, the adipose-derived stem cells are human adipose-derived stem cells. Preferably, the fibroblasts are normal human dermal fibroblasts. Preferably, the epidermal cells are normal human epidermal keratinocytes.SELECTED DRAWING: Figure 1

The invention discloses application of cationic polymer compounded bentonite flocculation for harvesting wide-salinity microalgae and a harvesting method, and belongs to the technical field of environmental protection. The method comprises the following steps: adding a polydimethyldiallylammonium chloride solution and a sodium bentonite suspension into an algae solution according to the cell biomass of microalgae to be harvested, stirring, settling and collecting to finish harvesting. According to the method disclosed by the invention, the microalgae in the wide-salt environment are harvested by combining PDDAC and BE for flocculation, the microalgae can be efficiently flocculated and harvested within the range of 0-40% of water salinity, and the harvesting rate can reach 90% or above; the flocculating efficiency is high, the dosage of a flocculating agent is small, the floc is large in size and compact, and the salt-resistant characteristic is remarkable; the method is suitable for efficient harvesting of microalgae in artificial culture or natural water under a wide salinity condition, provides a key technology for the field of microalgae harvesting, and has a wide application prospect; the problems of poor salinity tolerance of a flocculating agent, large dosage of the flocculating agent and poor harvesting effect in microalgae harvesting through flocculation at present are solved.

The invention discloses a wild bamboos fungus and a culture method thereof. The Gymnopus sp.lhf1475 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 11, 2024, the address is the 5th floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou, the postal code is 510070, and the preservation number is GDMCC No: 64861. According to the invention, the new species, namely the fungus Gymnopus sp.lhf1475, of the gymnopus is separated from a Guizhou gymnopus specimen (HGASMF01-23515), hyphae can be rapidly produced under an artificial culture condition, and the fungus has certain active substances and bacteriostatic action and has a relatively good application prospect.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

This invention relates to a rooting culture medium and tissue culture method for Rhizoma Cypripedium, belonging to the field of plant culture technology. The tissue culture method includes the following steps: obtaining explants, sterilizing them, transplanting them to a seedling culture medium for primary culture, subculturing, and then transplanting the subcultured explants to a rooting culture medium for rooting culture to obtain Rhizoma Cypripedium seedlings; the seedling culture medium and rooting culture medium are sugar-free media. This method enables the artificial culture of Rhizoma Cypripedium, with high rooting rate, high seedling establishment rate, and high survival rate of transplanted Rhizoma Cypripedium seedlings.

The invention relates to the technical field of biology, and discloses a bifunctional bacillus strain YJ211 and application thereof, the bifunctional bacillus strain YJ211 is bacillus sp. YJ211, the preservation number of the bifunctional bacillus strain YJ211 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M20251511, and the preservation date is July 2, 2025. The bacillus sp. YJ211 strain provided by the invention can be smoothly cultured on an artificial culture medium and is easy to obtain and breed, which is crucial for being used as a strain for degrading microplastics and preventing and treating cotton verticillium wilt, and is convenient to apply and popularize in industrial production. The YJ211 strain shows remarkable degradation capacity on PET (polyethylene terephthalate) micro-plastics, and the degradation efficiency of the YJ211 strain can reach 32.71%.

The present application belongs to the technical field of fish culture, and provides a large-specification Esxoleterus notatus fry culture method. The culture method comprises the following steps: pond pretreatment, fry release, feed feeding, water quality management, and disease prevention and treatment. The culture method is reasonable and simple, overcomes the low survival rate of artificial culture of Esxoleterus notatus in the prior art, is suitable for industrialized production, and has important significance for improving the comprehensive culture benefit of Esxoleterus notatus.