302 results about "Gonad" patented technology

The invention provides an ecology-imitating artificial propagation method for Dongquasichthys nobilis. The ecology-imitating artificial propagation method comprises the following steps: S1, selecting male and female parent fishes and putting the male and female parent fishes into an artificial ecology-imitating breeding pond for breeding; s2, in a non-breeding season, feeding female parent fish feed A for 2-3 times every day, and feeding male parent fish feed B for 2-3 times every day; s3, 2-3 months before breeding, female parent fish fortified feed I is fed 2-3 times every day, and male parent fish fortified feed II is fed 2-3 times every day; s4, after the gonad of the parent fish is mature, artificial spawning induction is conducted, and fertilized eggs are collected and hatched. According to the method, the ecology-imitating breeding environment is manufactured, so that the east astrostroma parent fish adapts to the artificial environment, the irritability is reduced, the east astrostroma parent fish is fed with different feeds in the non-breeding season and the breeding season, the ecological factor and nutritional requirements for gonad development of the east astrostroma can be met, the immunity of the east astrostroma parent fish is improved, gonad maturation is promoted, and the survival rate of the east astrostroma parent fish is increased. The quality of sperms and ova is improved, the fertilization probability is improved, the malformation probability of hatched larvae is reduced, and the breeding efficiency is improved.

The present disclosure relates to the technical field of marine fish cultivation and breeding, and in particular to an industrialized cultivation method for a cephalopholissonnerati fry. The method includes: a step of sequentially performing food acclimation and nutrient enrichment on a cephalopholissonnerati parent fish under conditions of 25-29° C. of water temperature and running water, to obtain the parent fish with mature gonad development, and other steps.

The invention discloses sea cucumber gonad-derived antioxidant peptide as well as a preparation method and application thereof, and belongs to the technical field of food biology. The antioxidant peptide is obtained by carrying out enzymolysis and molecular docking screening on sea cucumber gonads, and comprises at least one of peptides with amino acid sequences as shown in SEQ.ID.NO.1-4. Wherein the docking energy of the SEQ.ID.NO.1 and the receptor SOD is-7.5 kcal / mol, the docking energy of the SEQ.ID.NO.2 and the receptor SOD is-7.5 kcal / mol, the docking energy of the SEQ.ID.NO.3 and the receptor SOD is-7.4 kcal / mol, and the docking energy of the SEQ.ID.NO.4 and the receptor SOD is-7.2 kcal / mol. Tests prove that the antioxidant peptide has high antioxidant activity and has wide application space in the field of functional food.

The invention discloses a method for obtaining high-quality fertilized eggs of wild sal-family pseudosciaena crocea, and belongs to the technical field of aquatic animal breeding. The method comprises the following steps: capturing wild parent fishes in the breeding season of the sal-family pseudosciaena crocea and transporting the wild parent fishes to a farm; the method comprises the following steps: carrying out temporary culture and intensive culture on wild parent fishes, selecting male and female wild parent fishes with mature gonads, anesthetizing, and injecting artificial spawning induction mixed hormones; after artificial spawning mixed hormones are injected, ova and seminal fluid are collected, and high-quality fertilized ova are obtained through artificial insemination. According to the method, all the technical steps are associated with one another to form a whole, the high-quality fertilized eggs of the large yellow croakers of the wild sal ammoniac family can be successfully obtained, and the proportion of the obtained high-quality fertilized eggs reaches 45% or above. According to the method, the obtained high-quality fertilized eggs are adopted for fry hatching, the hatching rate of the obtained fertilized eggs reaches 62% or above, the fertilized eggs can be used for constructing and breeding a wild sal-family pseudosciaena crocea basic population, and artificial breeding of the sal-family pseudosciaena crocea is achieved.

The invention discloses a sebastes schlegeli testis cell line as well as a construction method and application thereof, and belongs to the technical field of marine fish cell culture. Primary culture and subculture are carried out on sebastes schlegeli juvenile fish testis tissues, so that the sebastes schlegeli testis cell line capable of being continuously subcultured in vitro for a long time is established. The sebastes schlegeli testis cell line established by the invention is in a fibroblast shape, has a good growth curve, and has relatively strong multiplication capacity. The lentiviral vector can significantly express the stromal cell marker gene temp2, can transfect the cell line, and can be subsequently applied to research of gonad development related functional genes. At present, the cell line has been subjected to in-vitro continuous passage for more than 60 generations, can be subjected to cryopreservation, and is high in recovery survival rate. Establishment of the cell line provides a stable and excellent experimental material for research of fish gonad development genes.

The invention provides a breeding environment regulation and control method for promoting rapid maturation of sexual glands of lateolabrax japonicus, and relates to the technical field of lateolabrax japonicus breeding environment regulation and control, and the method specifically comprises the following steps: under different breeding environment parameter combinations, respectively carrying out lateolabrax japonicus breeding tests, constructing a lateolabrax japonicus development prediction model, and obtaining growth state parameters and sexual gland development parameters of lateolabrax japonicus; the method comprises the following steps: establishing a functional relation among body length, body height, body weight and growth character coefficients, establishing a functional relation among gonad weight, gonad index and gonad development coefficients, generating gonad maturation coefficients, performing iterative optimization on the gonad maturation coefficients by adopting a genetic algorithm, screening out individuals corresponding to the gonad development degree reaching the IV stage, and establishing a functional relation among body length, body height, body weight and growth character coefficients. And taking the individual corresponding to the maximum value of the gonad maturation coefficient as an optimal breeding environment parameter combination. According to the method, the optimal breeding environment parameter combination is found out through iterative optimization of the genetic algorithm, so that the aim of promoting rapid maturation of sexual glands of lateolabrax japonicus is achieved, and the breeding efficiency and benefits are improved.

The present disclosure relates to the technical field of marine fish cultivation and breeding, and in particular to an industrialized cultivation method for a Cephalopholissonnerati fry. The method includes: a step of sequentially performing food acclimation and nutrient enrichment on a Cephalopholissonnerati parent fish under conditions of 25-29° C. of water temperature and running water, to obtain the parent fish with mature gonad development, and other steps.

The invention discloses an artificial breeding method of epinephelus fuscoguttatus and pink pterus coerulescens hybrid species, which comprises the following steps: screening healthy epinephelus fuscoguttatus as a female parent, screening healthy pink pterus coerulescens as a male parent, and carrying out enhanced breeding on the male parent and the female parent, the gonad maturation of the parent is promoted; the method comprises the following steps: collecting egg liquid of a female parent and semen of a male parent, inseminating to obtain a fertilized egg, incubating the fertilized egg to obtain a hybrid spot, performing authenticity identification on the hybrid spot by using one or more of sequences SEQ ID NO.1-SEQ ID NO.8, and determining that the hybrid spot inherits genetic information of the female parent and the male parent. The technical blank of crossbreeding of the pink pink bass as the parent grouper is filled, so that the hybrid obtained by hybridizing the epinephelus fuscoguttatus and the pink pink bass is reliable in authenticity, and inherits genetic information of male and female parents on the molecular level.

The invention discloses a method for inducing ostrea rivularis triploid, and relates to the technical field of shellfish genetic breeding. The specific preparation method comprises the following steps: firstly, obtaining egg liquid and seminal fluid from gonads of female and male ostrea rivularis individuals, cleaning and curing the egg liquid, and adding the seminal fluid into the egg liquid after cleaning to obtain a fertilized egg solution; when the proportion of the first polar bodies in the eggs of the fertilized egg solution is 30-50%, adding an inducer into the fertilized egg solution for induction and cleaning to obtain induced fertilized eggs; and finally, hatching and screening the induced fertilized eggs to obtain D-shaped larvae, and culturing to obtain the crassostrea rivularis triploid population. The method for inducing the crassostrea rivularis triploid is high in triploid induction rate, high in final hatching rate, capable of stably surviving and good in application prospect.

The invention belongs to the technical field of food biology, and particularly relates to sea cucumber gonad-derived anti-inflammatory peptide as well as a preparation method and application thereof. The anti-inflammatory peptide is obtained by performing enzymolysis and molecular docking screening on sexual glands of sea cucumbers, and comprises at least one of peptides with amino acid sequences as shown in SEQ.ID.NO.1-3. Experiments prove that the anti-inflammatory peptide has high anti-inflammatory activity, provides a basis for preparing functional food from natural food sources, and has important significance for development of novel anti-inflammatory products.

The invention provides an artificial breeding method of wild sal-family large yellow croaker, and belongs to the technical field of aquatic animal breeding. The invention provides an artificial breeding method of wild sal-family pseudosciaena crocea, which comprises the following steps: domesticating the wild sal-family pseudosciaena crocea, then carrying out intensive cultivation, and after the wild sal-family pseudosciaena crocea is cultivated until gonad is mature, carrying out artificial spawning induction and natural fertilization on the obtained parent fish of the sal-family pseudosciaena crocea to obtain fertilized eggs; the fertilized eggs are subjected to incubation and fry cultivation, and sal-family large yellow croaker fries are obtained; the artificial spawning method comprises the step of injecting hormone into parent fish in two steps. According to the method, favorable conditions are created for large-scale breeding of the sal-family pseudosciaena crocea, and a large number of artificially bred sal-family pseudosciaena crocea fry groups can be obtained by adopting the method.

The invention discloses a traditional Chinese medicine compound oral preparation for treating male oligoasthenospermia, and belongs to the technical field of traditional Chinese medicine preparations. The rhizoma polygonati processed with wine, the prepared rehmannia root and the like are used for nourishing the liver and kidney to consolidate congenital, the codonopsis pilosula, the astragalus membranaceus and the like are used for invigorating spleen and replenishing qi to complement postnatal, the fried cowherb seed is used for activating blood circulation, and the salted semen plantaginis is used for promoting diuresis, so that the problems of insufficient spermatogenesis and low vitality caused by deficiency of the spleen and the kidney are fundamentally solved. Experiments and clinical researches show that the preparation can significantly improve the sperm quantity, activity and activity rate of oligoasthenospermia model mice and patients, the clinical total effective rate reaches 90%, the pregnancy rate reaches 53.3%, and the curative effect is superior to that of Wuzi Yanzong pills in a control group. The action mechanism covers regulation of blood lipid metabolism, improvement of hypothalamus-pituitary-gonad axis functions, repair of testicular seminiferous tubule structural damage and reduction of lipid deposition, and meanwhile, oxidative stress and inflammatory injury are relieved and spermatogenic cell functions are protected by regulating and controlling a PPAR signal channel and inhibiting molecular pathways such as Cga / Sting mediated cell apoptosis.

The invention belongs to the technical field of modern agriculture, and particularly relates to a method for creating pseudo male trachinotus ovatus. According to the method for creating the pseudo male trachinotus ovatus, alpha-naphthoflavone and 17 alpha-methyltestosterone are used in a combined mode, feeding induction is started from the 2-month-old period, and finally sperms can be observed in sexual glands of the genetic female trachinotus ovatus in the 8-month-old period. According to the method, the sexual gland of the genetic female trachinotus ovatus (the sexual gland normally develops into an ovary) can be reversed into the testis, in the 8-month-old period, the observed reversal efficiency is 63.6%, compared with single use of alpha-naphthoflavone, the sexual reversal proportion is increased by more than one time, the number and maturity of sperms of the pseudo male trachinotus ovatus are remarkably increased, and the sexual efficiency of the trachinotus ovatus is improved. And the success rate of subsequent all-female golden pomfret creation is greatly improved.

The invention provides a method for overspeed preparation of gene edited goldfish. The method comprises the following steps: S1, obtaining gobiocypris rarus without primordial germ cells as receptor fish; s2, designing a target spot for the goldfish amh gene, introducing a Cas9 / gRNA complex into a goldfish fertilized egg, performing sequencing verification to obtain a mutant individual, and feeding the mutant individual as a donor fish; s3, taking gonads of donor fish, cutting into pieces, performing enzymolysis digestion, filtering, and performing density gradient centrifugation to enrich donor germline stem cells; s4, transplanting the germline stem cells of the donor fish into the receptor fish body, and feeding to obtain the gene edited goldfish sperms and ova. S5, hybridizing and feeding goldfish amh mutant sperms and ova produced by gobiocypris rarus to obtain gene edited goldfish individuals. According to the invention, gobiocypris gobiocypris is used as a receptor, the cross-subfamily gene edited goldfish is used as a donor, and an efficient, stable and easy-to-popularize method for preparing the gene edited goldfish is provided by utilizing a belly borrowing reproduction technology.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention discloses a zebra fish spermatogonial stem cell separation and transplantation method, and belongs to the technical field of fish germline stem cell separation. The method comprises the following steps: washing complete sexual glands of zebra fish testis by adopting penicillin / streptomycin double-antibody-containing PBS (Phosphate Buffer Solution), physically shearing, digesting and dissociating, and filtering by a screen, so as to obtain zebra fish testis cell suspension; performing density gradient centrifugation to obtain zebra fish spermatogonial stem cells; dyeing, marking, centrifuging, washing, re-suspending, and carrying out micro-injection transplantation to enter the back of the abdominal cavity of the acceptor zebrafish juvenile fish. According to different physical properties of different cells, spermatogonial stem cells are separated, purified and transplanted from zebra fish testis cell suspension by adopting a density gradient centrifugation method, and migration and proliferation processes of donor germ cells can be observed in receptor fish after fluorescence labeling. And a new technical path is provided for fish germplasm resource preservation, endangered fish protection, economic cultured fish yield increase and new variety cultivation.

The invention discloses an efficient artificial breeding and intermediate cultivation method for holothuria leucospilota. The holothuria leucospilota with mature gonad is firstly dried in the shade and then transferred into seawater, sodium sulfite is added to make water oxygen deficit, and spawning induction is stimulated. According to the physiological habits of the holothuria leucospilota, hypoxia suffocation stimulation is carried out on the holothuria leucospilota by using sodium sulfite, and a very good spawning induction effect is achieved. Compared with an existing traditional tropical sea cucumber artificial spawning induction method, such as a running water stimulation method and a shade drying method, with the highest spawning induction rate of 13.3-18.0%, the effect of sea cucumber spawning induction is more obvious (the spawning induction rate reaches up to 75-81%) by means of the method. The sodium sulfite has no obvious toxicity to the parent sea cucumber and is low in price; a traditional spawning induction method must be operated at night, but the method has no requirement for spawning induction time, has the advantages of being high in operability, easy to popularize and capable of reducing parent parameter quantity and providing stable fertilized egg supply for large-scale sea cucumber breeding.

The present disclosure provides a one-piece garment for protection of a wearer from radiation. The garment comprises an upper body section, a collar section and a front flap section to shield a region of thyroid, a region of torso and a region of gonad of the wearer.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to sexual gland weight traits of fugu rubripes and application of the SNP molecular marker, and relates to the technical field of biology. According to the invention, association analysis is carried out in combination with the sexual gland heavy character of the fugu rubripes, and the SNP molecular marker significantly related to the sexual gland heavy character of the fugu rubripes is successfully excavated. The SNP molecular marker is used for breeding of improved gonad breeds of fugu rubripes, and compared with traditional phenotype-based selective breeding and cross breeding means, the SNP molecular marker has higher accuracy and detection efficiency and has a wide application prospect.

The invention discloses a feed additive for promoting muscle and gonad development of eriocheir sinensis and application of the feed additive, and relates to the technical field of aquatic feed. The feed additive comprises cholesterol and omega-3 high unsaturated fatty acid. Each kilogram of the feed comprises the following feed additives by weight: 0.4 to 4.5 g of cholesterol and 3.5 to 4.5 g of omega-3 high unsaturated fatty acid. According to the feed additive, 4.022 g / kg omega-3 high unsaturated fatty acid (omega-3 HUFA) is supplemented into feed containing 4.114 g / kg cholesterol, so that the weight gain rate, the gonad index and the meat percentage of female crabs in the fattening period can be remarkably increased; the protein nutrition deposition capability of gonads and muscles is obviously improved; when the feed added with the feed additive is used in the fattening period of the eriocheir sinensis, the feed additive has importance on nutrient deposition and quality improvement of female crabs; the production cost of farmers can be reduced, the product value is improved, and technical support is provided for healthy development of the hairy crab industry.

The invention discloses a functional gel feed for purple sea urchins and a preparation method of the functional gel feed. Wherein the mass fraction of the 5-HT in the functional gel feed is 0.05%-0.05%, and the energy of the functional gel feed is 1800-2000 kcal / kg; after the functional gel feed is used for feeding the purple sea urchins for 20-40 days, the egg yolk protein content of the purple sea urchins is 400-497 mg / g; and the gonad index is 9-13%. On one hand, the functional gel feed disclosed by the invention is balanced in nutrition, rich in various nutrient substances required by the growth of the purple sea urchins, capable of meeting the growth and development requirements of the purple sea urchins, high in feed digestibility and capable of reducing excrement discharge and reducing pollution of breeding to the environment; and on the other hand, the feed is gel-shaped, is good in palatability and is easy to eat by the purple sea urchins. In addition, the functional gel feed is good in stability in water and not prone to loss, and the utilization rate of the feed can be increased.

The invention relates to a method for editing target genes of prawn filial generations by exopalaemon carinicauda or vannabine and application of the method. Based on the gonad development and reproduction characteristics of shrimps and crabs, Cas9 RNP (a mixture formed by mixing sgRNA and Cas9 ribonucleoprotein) is directly injected into bodies of exopalaemon carinicauda or litopenaeus vannamei with developed ovaries in an intramuscular injection manner. The used sgRNA is a guide RNA (Ribonucleic Acid), namely EcIAG-gRNA or LvIAG-gRNA, which is designed on the basis of an IAG gene (EcIAG or LvIAG) of exopalaemon carinicauda or litopenaeus vannamei. Different gRNAs and Cas9 proteins are respectively mixed and then injected into an exopalaemon carinicauda or litopenaeus vannamei parent body through intramuscular injection, Cas9 RNP can enter an ovary along with blood circulation and enter an oocyte along with development of the ovary, editing of a target gene on a genome is realized, and after the edited oocyte develops into an ovum, a progeny edited mutant is formed through fertilization. The method can also be used for delivering other biological macromolecular substances to oocytes of decapod crustacean such as exopalaemon carinicauda or litopenaeus vannamei.

The present invention relates to a method for obtaining a genetically modified progeny of an avian animal by packaging a gene edited or genetically modified vector in a recombinant adenovirus and injecting into the dorsal aorta of an embryo when PGCs migrate to a developing gonad; compared with the existing disclosed method for transforming the PGCs in vitro, the operation method disclosed by the invention not only can stably and effectively infect the PGCs in vivo, but also has the advantages that more importantly, the probability of generating gene editing offspring is higher, and particularly, the breeding efficiency of cultivating the transgenic chicken offspring with KRT75L4 gene and MSTN gene knockout is higher; and on the other hand, the reporter gene or crispr-cas protein gene does not need to be integrated into the genome of the bird and the non-native protein does not need to be expressed, so that the operability is higher from the commercial level.

The invention relates to the technical field of animal husbandry, in particular to a synchronous oestrus-timing insemination method suitable for cattle, which comprises the following steps: firstly, performing intramuscular injection of PG (cloprostenol sodium) at different time points after calving to promote uterine recovery and luteolysis and lay a synchronous oestrus foundation; the method comprises the key steps that GnRH (gonadotropin release hormone) is given through intramuscular injection on the 43rd day, the 53rd day, the 60th day and the 69th day, pituitary is promoted to release follicle-stimulating hormone (FSH) and luteinizing hormone (LH), collection and screening of follicles are guided, follicle maturation is promoted, the oestrus uncovering rate and the oestrus conception rate are increased, a melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day, and the melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day. The synthesis of ovarian hormones is promoted by regulating the hypothalamus-pituitary-gonad axis, the secretion of follicle cell factors and maturation promoting factors is promoted, the development of granular cells is promoted, and the maturation of oocytes is promoted. According to the method, the pregnancy rate of a double-synchronization-timing insemination scheme which is most widely used in a large-scale dairy cow breeding pasture at present can be remarkably increased, and feasible technical support is provided for success of increasing the breeding efficiency of cows.

The present invention discloses a feed additive for secondary spawning of red claw crayfish, as well as its preparation method and application. The present invention comprises the following components: 29.5-30.5% bait powder, 24.5-25.5% methyl farnesate, 9.5-10.5% schizochytrium powder, 9.5-10.5% Porphyridium powder, 7-8% 5-hydroxytryptamine creatinine sulfate, 9.5-10.5% linseed oil, and 7-8% alanine hydroxamic acid; the bait powder is a mixture of lugworm powder and squid viscera powder in a weight ratio of 4-6:5. The present invention also provides a preparation method for the above-mentioned feed additive. The feed additive of the present invention is added to conventional shrimp feed to prepare a compound shrimp feed for feeding the first batch of spawning shrimp of red claw crayfish. The present invention can significantly promote the gonadal development of spawning shrimp of red claw crayfish, rapidly mature their ovaries in a short period of time, improve the spawning rate of spawning shrimp obtained therefrom, and thereby increase the supply of shrimp fry.

The present invention relates to the technical field of genetic breeding of marine economic shellfish, and in particular to a breeding method for a new Vibrio-resistant variety of scorpion clams. Specifically, the method comprises the following steps: using a semi-lethal concentration of Vibrio parahaemolyticus to stress adult scorpion clams, recovering 15% of the surviving individuals, and selecting scorpion clams with an 8% seed retention rate to obtain Vibrio-resistant scorpion clams, fattening the scorpion clams in an indoor workshop to mature the gonads, and inducing the scorpion clams to produce heritable immune factors by inactivating Vibrio parahaemolyticus before inducing spawning; then inducing the scorpion clams to spawn, obtaining the Vibrio-resistant strain of the F1 generation of scorpion clams, and then rapidly cultivating the scorpion clams to sexual maturity by adopting the breeding and culture technology of "breeding in the north and raising in the south" for the F1 generation; thereafter, selecting scorpion clams by stress, enhancing the immunity of the offspring, and continuously breeding by the method of "breeding in the north and raising in the south" every year to obtain a new Vibrio-resistant variety of scorpion clams with stable genetics and excellent traits. The method of the invention can significantly improve the anti-vibrio ability and the aquaculture survival rate of razor clams, and improve the good variety preservation and supply capacity of the razor clams aquaculture industry.

The invention relates to the technical field of biological medicines, and particularly discloses application of ebbeselenium in preparation of a medicine for treating male delayed gonadal hypofunction. After the ebbeselenium is adopted for intervention, the autophagy activity of testicular interstitial cells of old mice is increased, lipid autophagy is enhanced, testosterone synthesis is promoted, the serum testosterone level is remarkably improved, and the symptoms of male delayed hypogonadism (LOH) are effectively relieved; ebbeselenium simultaneously shows a relatively good delaying effect on senescence of a plurality of organs. The ebbeselenium can improve the reproductive capacity, the behavior memory capacity, the athletic capacity and the metabolic function of old mice at the same time, and synergistic and effective treatment of low serum testosterone and LOH complications is achieved. Besides, by enhancing the molecular reduction effect of PRDX1 targeting ATG4B, the ebbeselenium regulates and controls the autophagy pathway of testicular interstitial cells and enhances the functions of the testicular interstitial cells so as to promote synthesis of testosterone, and a new treatment strategy is provided for treatment of LOH.

The invention relates to the technical field of pomacea canaliculata resourceful treatment and aquaculture, in particular to a macrobrachium rosenbergii breeding method based on pomacea canaliculata resourceful treatment. The method comprises the following steps: sequentially carrying out multi-stage purification on captured ampullaria gigas through temporary culture emptying, circulating micro-flowing water washing and EM bacterial liquid soaking, then integrally crushing the ampullaria gigas into ampullaria gigas meat paste, adding table salt and / or organic acid, and then mixing the ampullaria gigas meat paste with a vitamin-rich plant raw material and a vitamin mineral premix according to a ratio; and the soft pomacea canaliculata block bait which is not easy to foam and scatter is formed by extrusion or compression molding in cooperation with a hydrophilic gel binder. In a breeding pond of the macrobrachium rosenbergii, the soft block bait is matched with compound feed for the parent macrobrachium rosenbergii for precise feeding according to a limited proportion and a limited period of time, and the feeding amount and water change are dynamically adjusted in combination with water quality indexes, so that the gonad development and reproductive performance of the macrobrachium rosenbergii are improved, the water quality is stabilized, and the bait cost is reduced.

A method of treating male hypogonadism in a subject in need thereof includes administering to the subject testicular organoids generated from isolated male progenitor cells. The method can be used to slow or halt the progression of age-related diseases, and increase healthspan and longevity, in hypogonadal males. The method can be used with subjects that have not or are not receiving testosterone replacement therapy. The method does not require that the subject is pre-treated with CG. The testicular organoid includes cells expressing GFRA1, VEGF, VEGFR2, PDGF-Rα, Cyp11A1, 3β-HSD, 17β-HSD, LHCGR, α-SMA, CD11b, MHC CII, CD64, CD95, Sox9, AR, inhibin β-B, and FSHR.