100 results about "Gonad" patented technology

The invention provides a method for overspeed preparation of gene edited goldfish. The method comprises the following steps: S1, obtaining gobiocypris rarus without primordial germ cells as receptor fish; s2, designing a target spot for the goldfish amh gene, introducing a Cas9 / gRNA complex into a goldfish fertilized egg, performing sequencing verification to obtain a mutant individual, and feeding the mutant individual as a donor fish; s3, taking gonads of donor fish, cutting into pieces, performing enzymolysis digestion, filtering, and performing density gradient centrifugation to enrich donor germline stem cells; s4, transplanting the germline stem cells of the donor fish into the receptor fish body, and feeding to obtain the gene edited goldfish sperms and ova. S5, hybridizing and feeding goldfish amh mutant sperms and ova produced by gobiocypris rarus to obtain gene edited goldfish individuals. According to the invention, gobiocypris gobiocypris is used as a receptor, the cross-subfamily gene edited goldfish is used as a donor, and an efficient, stable and easy-to-popularize method for preparing the gene edited goldfish is provided by utilizing a belly borrowing reproduction technology.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to sexual gland weight traits of fugu rubripes and application of the SNP molecular marker, and relates to the technical field of biology. According to the invention, association analysis is carried out in combination with the sexual gland heavy character of the fugu rubripes, and the SNP molecular marker significantly related to the sexual gland heavy character of the fugu rubripes is successfully excavated. The SNP molecular marker is used for breeding of improved gonad breeds of fugu rubripes, and compared with traditional phenotype-based selective breeding and cross breeding means, the SNP molecular marker has higher accuracy and detection efficiency and has a wide application prospect.

The invention relates to the technical field of animal husbandry, in particular to a synchronous oestrus-timing insemination method suitable for cattle, which comprises the following steps: firstly, performing intramuscular injection of PG (cloprostenol sodium) at different time points after calving to promote uterine recovery and luteolysis and lay a synchronous oestrus foundation; the method comprises the key steps that GnRH (gonadotropin release hormone) is given through intramuscular injection on the 43rd day, the 53rd day, the 60th day and the 69th day, pituitary is promoted to release follicle-stimulating hormone (FSH) and luteinizing hormone (LH), collection and screening of follicles are guided, follicle maturation is promoted, the oestrus uncovering rate and the oestrus conception rate are increased, a melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day, and the melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day. The synthesis of ovarian hormones is promoted by regulating the hypothalamus-pituitary-gonad axis, the secretion of follicle cell factors and maturation promoting factors is promoted, the development of granular cells is promoted, and the maturation of oocytes is promoted. According to the method, the pregnancy rate of a double-synchronization-timing insemination scheme which is most widely used in a large-scale dairy cow breeding pasture at present can be remarkably increased, and feasible technical support is provided for success of increasing the breeding efficiency of cows.

The invention relates to the technical field of pomacea canaliculata resourceful treatment and aquaculture, in particular to a macrobrachium rosenbergii breeding method based on pomacea canaliculata resourceful treatment. The method comprises the following steps: sequentially carrying out multi-stage purification on captured ampullaria gigas through temporary culture emptying, circulating micro-flowing water washing and EM bacterial liquid soaking, then integrally crushing the ampullaria gigas into ampullaria gigas meat paste, adding table salt and / or organic acid, and then mixing the ampullaria gigas meat paste with a vitamin-rich plant raw material and a vitamin mineral premix according to a ratio; and the soft pomacea canaliculata block bait which is not easy to foam and scatter is formed by extrusion or compression molding in cooperation with a hydrophilic gel binder. In a breeding pond of the macrobrachium rosenbergii, the soft block bait is matched with compound feed for the parent macrobrachium rosenbergii for precise feeding according to a limited proportion and a limited period of time, and the feeding amount and water change are dynamically adjusted in combination with water quality indexes, so that the gonad development and reproductive performance of the macrobrachium rosenbergii are improved, the water quality is stabilized, and the bait cost is reduced.

A method of treating male hypogonadism in a subject in need thereof includes administering to the subject testicular organoids generated from isolated male progenitor cells. The method can be used to slow or halt the progression of age-related diseases, and increase healthspan and longevity, in hypogonadal males. The method can be used with subjects that have not or are not receiving testosterone replacement therapy. The method does not require that the subject is pre-treated with CG. The testicular organoid includes cells expressing GFRA1, VEGF, VEGFR2, PDGF-Rα, Cyp11A1, 3β-HSD, 17β-HSD, LHCGR, α-SMA, CD11b, MHC CII, CD64, CD95, Sox9, AR, inhibin β-B, and FSHR.

The application discloses a kind of molecular markers for identifying genetic sex of blue fish and its application, belong to the field of aquatic animal genetics and breeding.The molecular marker includes nucleotide sequence such as the common band of blue fish female and male fish shown in SEQ ID NO:1 and nucleotide sequence such as the unique band of blue fish male shown in SEQ ID NO:2, and the identification method comprises: taking the DNA of the blue fish sample to be identified as template for PCR amplification, after the band of PCR product is determined by agarose electrophoresis, capillary electrophoresis is carried out, if signal appears at 152bp and 168bp simultaneously, then it is male;If only signal appears at 168bp, then it is female.The molecular marker of the application can accurately obtain the genetic sex of different blue fish populations, has the advantages such as simple, fast, efficient, not affected by early undifferentiated gonad of blue fish, etc., is used for blue fish marker assisted breeding, which greatly saves the cost of aquaculture and improves the breeding efficiency.

The utility model particularly relates to an acrossocheilus fasciatus artificial induction spawning inspection fish nest, and belongs to the technical field of fish culture, the fish nest comprises a box body, a microflow assembly and a gravel layer, the box body is provided with a containing cavity, and the box body comprises at least one inlet and outlet which is formed in the side surface of the box body; the microflow assembly is connected with the box body, the microflow assembly is provided with a microflow path at least partially extending and penetrating through the microflow assembly, and the microflow path is communicated with the external space and a water area of the containing cavity and used for forming micro water flow; the gravel layer is arranged in the containing cavity. The spawning site can be provided for acrossocheilus fasciatus, acrossocheilus fasciatus in the oestrus period is artificially induced to enter the spawning site to prepare spawning, acrossocheilus fasciatus with mature gonad can be caught for inspection, artificial insemination work can be carried out timely, operation is convenient, and the manual labor intensity can be reduced.

The invention discloses an artificial breeding method of lota lota, which comprises the following steps: S1, selecting robust lota lota as parent fish, placing the parent fish in an indoor culture tank for temporary culture, and inducing gonad to develop mature by regulating and controlling water temperature; s2, performing artificial spawning induction on the parent fish with mature gonad to obtain ova and semen, and performing in-vitro fertilization on the ova and the semen to obtain fertilized ova; s3, performing two-step soaking treatment on the fertilized eggs by using a composite biological functional liquid containing a liquid A and a liquid B, and then transferring the fertilized eggs into a hatching facility for hatching to obtain fish fries; and S4, breeding the hatched fish fries. According to the artificial breeding method of the lota lota, the survival rate of fertilized eggs and the survival rate in the fry breeding stage are increased to a high level, large-scale and controllable artificial breeding of the lota lota becomes possible, and the overall technical scheme has high stability and industrial application value.

This invention discloses the application of ethinylestradiol and drospirenone in the preparation of drugs for treating post-traumatic stress disorder (PTSD). Ethinylestradiol, as an estrogen receptor agonist, regulates the hypothalamic-pituitary-gonadal axis and is used to treat female hypogonadism, menopausal syndrome, and advanced breast cancer, often in combination with progestins as oral contraceptives. The use of drospirenone and ethinylestradiol tablets (II) after induced abortion can shorten vaginal bleeding time, promote endometrial repair, and reduce the incidence of postoperative complications and repeat miscarriages. This invention has found that ethinylestradiol and drospirenone can reverse the abnormal decrease in hormones and neurosteroids caused by PTSD after spontaneous abortion, particularly the abnormal decrease in serotonin and tetrahydroprogesterone.

This invention discloses a method for constructing a fast-growing, sterile zebrafish model, belonging to the field of fish animal model preparation technology. This invention is the first to discover the role of the CK gene in regulating fish growth rate and fertility: fish with simultaneous knockout of ck1 and ck2 genes exhibit rapid growth and gonadal abortion. Using the method provided by this invention, a fast-growing, sterile zebrafish model was successfully constructed. These zebrafish exhibited gonadal dysplasia and sterility, but also rapid growth. Compared to wild-type zebrafish, the 4-month-old double mutant zebrafish showed significantly increased body length and weight. The disclosed gene function and zebrafish model construction method can be used for breeding fast-growing, sterile fish varieties and studying related mechanisms, which is of great significance for improving the economic benefits of aquaculture and protecting ecological security.

The invention provides a preparation method of a culture solution for promoting gonad development of pinctada maxima. According to the preparation method of the culture solution for promoting gonad development of the pinctada maxima, Chinese herbal medicine components such as the radix isatidis and the honeysuckle flowers are blended and the proportion is optimized, so that gonad maturation of the pinctada maxima can be directionally stimulated, harmful bacteria in a culture environment can be inhibited, the culture survival rate is remarkably increased, and the double benefits of promoting development and preventing diseases are achieved; the selected Chinese herbal medicines are free of toxic and side effects, free of hormone residues, free of environmental pollution during use, free of drug accumulation in pinctada maxima bodies, easy and convenient to operate, low in cost and extremely high in popularization value. A traditional culture solution formula system is further optimized, efficient application of Chinese herbal medicine ingredients in the field is achieved for the first time, the disease outbreak risk in the breeding process is effectively avoided, and a solid theoretical support and practical reference are provided for the gonad development promoting technology in large-scale breeding of the pinctada maxima.

The invention belongs to the technical field of aquaculture, and particularly relates to a decapterus maruadsi breeding feed and application thereof in improving the quality of decapterus maruadsi fertilized eggs. The invention provides a decapterus maruadsi breeding feed. The feed comprises a first feed and a second feed which are mutually independent, the first feed comprises a basal feed, fish cream and a nutrition enhancer; the second feed comprises the first feed and oyster meat; the nutrition enhancer contains calcium pantothenate, vitamin B1, vitamin B2, vitamin B6, vitamin C and vitamin F. When the culture feed for the decapterus maruadsi is used for performing nutrient enrichment on parent fishes of the decapterus maruadsi, synchronous development of sexual glands of male and female parent fishes of the decapterus maruadsi can be promoted, the quality of fertilized eggs of the decapterus maruadsi is improved, the good egg rate of the fertilized eggs of the decapterus maruadsi reaches 88.50% and is 39.15% higher than that of a control group, and the fertilization rate of the fertilized eggs reaches 79.58% and is 19.46% higher than that of the control group.

The invention discloses an artificial maturity promoting and fry breeding method for Peltella corii, which comprises the steps of cooling temporary rearing, maturity promoting, mating, spawning, hatching, high-density larva breeding, metamorphosis and the like, male snails and female snails are separately bred during maturity promoting, and a breeding scheme is optimized. According to the technical scheme, batch gonad maturation of parent snails according to plans is achieved, and the maturity is good; high-density breeding technologies such as fertilized egg hatching and larva breeding are overcome, a stable and efficient fry production process is established, a large number of high-quality, healthy and regular-specification young snails are provided for the breeding industry, and the provenance basis of the industry is fundamentally guaranteed.

The invention relates to the technical field of aquaculture feed, in particular to a feed additive for improving ovary color of scylla paramamosain and application of the feed additive. The feed additive is prepared from the following raw material components in parts by mass: 30-60 parts of Chinese wolfberry fruit powder, 10-30 parts of a marigold extract, 2-8 parts of chenodeoxycholic acid and 5-15 parts of vitamin E powder. The additive systematically solves the technical bottlenecks of low absorptivity and unstable deposition of natural pigments in crab bodies through the synergistic effect of all the components. When the feed additive is added into feed for fattening female scylla paramamosain, the ovary redness value can be remarkably increased, the color is rich and natural, and meanwhile, the growth of parent crabs and the development of gonad are promoted. The feed is completely prepared from natural raw materials, is safe, free of residues and controllable in cost, and provides an effective scheme for realizing high-valued and green cultivation of scylla paramamosain'red cream crab '.

The invention discloses a method for identifying germ cells of grass carps and carps by using dazl genes. The method comprises the following steps: cloning dazl cDNA sequences of the grass carps and the carps, and performing comparative analysis by using DNAMAN software; designing a universal non-specific primer, a grass carp specific primer and a carp specific primer according to sequence differences; extracting total RNA (Ribonucleic Acid) of testis of 12-month-old grass carp and carp, and reversely transcribing into cDNA Respectively carrying out PCR (Polymerase Chain Reaction) amplification by using three pairs of primers by taking the cDNA as a template; carrying out 1% agarose gel electrophoresis and imaging on the PCR product, and identifying and distinguishing germ cells according to the size of a strip; the method is simple in structure and reasonable in design, high-specificity primers are designed based on small difference of dazl gene sequences for the first time, precise amplification is achieved by combining with optimized annealing temperature (60 DEG C / 65 DEG C), special instruments are not needed, operation is easy and convenient, sensitivity is high, grass carp germ cells in carp gonads after transplantation can be rapidly identified, development of the'belly borrowing reproduction 'technology is supported, and the method is suitable for large-scale popularization and application. The grass carp breeding period is remarkably shortened, and the important industrialization prospect is achieved.

The invention discloses a recirculating water breeding system for simulating natural ocean current and a production promoting method, and belongs to the technical field of aquaculture engineering. The invention discloses a circulating water breeding system and a production promoting method for simulating natural ocean current. The system comprises a culture pond body structure, a bionic water flow stimulation subsystem, a circulating water treatment subsystem and an intelligent control subsystem, the method comprises the following steps of: preparing the system in a pond entering stage of parent fish of the Dongquasichthys nobilis, a gonad development promoting stage, an oviposition inducing stage and a postpartum management stage. The problems that in the prior art, a water flow mode is single and fixed, a gonad development stimulation mode is extensive, artificial insemination is relied on, the automation degree is low, and the overall breeding efficiency is low are solved. According to the method, an integrated circulating water culture environment is constructed, and complex tide and ocean current conditions of a natural spawning site of the Dongcao are accurately simulated, so that gonad development synchronization of parent fishes is effectively stimulated, the natural breeding behavior of the parent fishes is triggered, and the spawning amount and the fertilization rate are remarkably increased.

The invention discloses an oryzias latipe spermatogonial stem cell strain capable of stably expressing Cas9 protein as well as a construction method and application of the oryzias latipe spermatogonial stem cell strain. The oryzias latipe spermatogonial stem cell strain is designed and constructed based on a Tol2 transposon system, wherein the transposon vector comprises a coding sequence of a Cas9 gene, a hygromycin B resistance gene and left and right arm sequences of a Tol2 transposon; constructing a Tol2 transposase expression vector; co-transfecting the transposon vector and the expression vector to the spermatogonial stem cells SG3 of the oryzias latipe; screening in an ESM4 culture medium containing hygromycin B to obtain a drug-resistant cell population capable of stably expressing Cas9; selecting monoclonal colonies under a microscope, and carrying out continuous subculture to obtain an oryzias latipe spermatogonial stem cell strain which stably expresses Cas9 protein and is named as oryzias latipe gonad cells SG3-Cas9. The method has the advantages of short construction period, high efficiency, good expression stability and the like, and provides a reliable cell platform for fish cell level gene editing research.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to sexual gland weight traits of fugu rubripes and application of the SNP molecular marker, and relates to the technical field of biology. According to the invention, association analysis is carried out in combination with the sexual gland heavy character of the fugu rubripes, and the SNP molecular marker significantly related to the sexual gland heavy character of the fugu rubripes is successfully excavated. The SNP molecular marker is used for breeding of improved gonad breeds of fugu rubripes, and compared with traditional phenotype-based selective breeding and cross breeding means, the SNP molecular marker has higher accuracy and detection efficiency and has a wide application prospect.

The invention relates to the technical field of aquaculture, in particular to a method for improving the growth performance and nutritional quality of female eriocheir sinensis. The method comprises the following steps: pretreating phaeodactylum tricornutum to obtain pretreated phaeodactylum tricornutum; in the feeding process of female eriocheir sinensis, basic feed is fed into a feeding water body, and the pretreated phaeodactylum tricornutum is additionally fed; wherein the feeding amount of the pretreated phaeodactylum tricornutum is dynamically adjusted according to the change of the feeding water temperature. By adding the phaeodactylum tricornutum, the food source of the eriocheir sinensis is directly changed, and the final weight, gonad index, meat percentage, total edible rate and fatness of female crabs are remarkably improved; protein, amino acid and vitamins which are rich in phaeodactylum tricornutum can directly optimize the growth and quality of the eriocheir sinensis, and especially under the condition of high-density culture, the quality of the eriocheir sinensis is correspondingly improved while the growth performance of the eriocheir sinensis can be remarkably improved.

The invention discloses a method for improving the quality of male sperms. Belongs to the technical field of biological medicine. It is found that the sperm quality of male mice can be remarkably improved by long-term in-vivo application of D-mannose. The method is mainly embodied in that kinematics parameters of total sperm motility rate and forward motility rate are remarkably increased, and the effect is independent of gonad development improvement; if the sperm mitochondrial membrane potential rises, the energy metabolism function is improved; the sperm acrosome integrity is improved, and the sperm quality is integrally optimized. The results show that D-mannose, as a safe natural monosaccharide, can significantly improve the quality of male sperms in an in-vivo intervention manner, and provides a new method and approach for treatment and intervention of male infertility, especially asthenozoospermia.

This invention discloses an artificial breeding method for the Oriental blackfish, belonging to the field of fish artificial breeding technology. It includes six core steps: broodstock selection and domestication, enhanced maturation cultivation, broodstock selection and anesthesia, spawning induction, artificial insemination, fertilized egg hatching, and fry rearing. One year in advance, suitable-aged wild Oriental blackfish are selected and domesticated in a flowing water pond. Before breeding, tocopherol is mixed into their feed to enhance gonadal development. High-quality broodstock are selected at a female-to-male ratio of 1:2 and anesthetized. A specific ratio of spawning inducing agent is injected using a two-injection method. Artificial insemination is performed in a standardized fiberglass circular fishpond under controlled water flow conditions. Fertilized eggs are incubated at 26±1℃ for 22 hours, and after hatching, they are transferred to a sterilized micro-flow water pond for rearing. This invention achieves a 100% spawning efficiency, a stable fertilization rate of over 95%, and a hatching rate exceeding 82%. It is simple to operate, cost-effective, and allows for large-scale breeding, providing key technical support for the conservation and population expansion of the Oriental blackfish.

The invention belongs to the technical field of biology, and particularly relates to a pharmaceutical composition for inducing gonad exhaustion of paralichthys olivaceus and application of the pharmaceutical composition. The pharmaceutical composition comprises docetaxel and cis-platinum, the mass ratio of docetaxel to cis-platinum is 1: 1, and the total concentration of docetaxel and cis-platinum is 100-200 mu g / mL. By optimizing the medicine proportion and the administration scheme, the problems of low survival rate and large injury of fish bodies in the existing gonad exhaustion method are solved, and efficient and stable preparation of the paralichthys olivaceus germline stem cell transplantation receptor can be realized. By controlling the ratio of docetaxel to cis-platinum, the administration concentration, the injection frequency, the action period and the like, the invention aims to solve the problems of insufficient exhaustion effect, low fish survival rate or serious gonad injury and the like in the existing receptor preparation, and provides reliable technical support for establishing a stable and efficient paralichthys olivaceus germline stem cell transplantation receptor system.

The present invention provides an endogenous germ cell-free fish, crustacean or mollusk having a chimeric gonad wherein the chimeric gonad comprises at least one transplanted germ cell, the transplanted germ cells have mutations that are present in the germ cell line and disrupt the development and / or function of somatic gonad cells that can be used as parent fish. The present invention also provides a method of preparing a parent fish, crustacean or mollusk for the production of sterilized fish, crustacean or mollusk that is sterilized or sex-determined, and the parent fish themselves.

The invention discloses a crossbreeding method for breeding pseudosciaena crocea in spring and summer and breeding pseudosciaena crocea in autumn. The crossbreeding method comprises the following steps: 1) screening high-quality parents of a spring and summer breeding group and an autumn breeding group; (2) adjusting the temperature and the illumination period, simultaneously carrying out nutrient enrichment, and carrying out reproduction regulation on the parent fish so as to synchronize gonad development of the parent fish; 3) performing artificial spawning induction by utilizing exogenous hormone, and performing natural hybrid fertilization or artificial dry-method fertilization according to a hybrid combination of breeding female fish in spring and summer, breeding male fish in autumn and breeding female fish in autumn and breeding male fish in spring and summer; 4, the obtained fertilized eggs are hatched and subjected to fry rearing, and finally the hybrid F1 new germplasm is obtained.The directional controllable hybridization of the two breeding populations is achieved, the genetic diversity of the large yellow croaker is increased, the germplasm degeneration problem is solved, and the filial generation has remarkable advantages in the aspects of growth speed, gonad development and the like.

To solve the problem that when the outer shell of a sea urchin main body is broken for conventional collection in the collection of the gonad of the sea urchin main body, the gonad is broken by an inserted conventional sea urchin breaker and the intestine connected to the mouth is broken. In addition, the sea urchin shell after collection has to be disposed of as industrial wastes for a fee.SOLUTION: When the gonad of the main body of the sea urchin is collected, the upper surface of the outer shell on the mouth side of the main body of the sea urchin is cut in a circular lid shape together with the mouth by the lowering and swinging operation of the cutting bit, so that the gonad and the internal organs of the main body of the sea urchin can be collected without being broken. Since the outer shell of the sea urchin shell is cut off in a lid shape and is not damaged, the sea urchin shell can be provided as a vessel.SELECTED DRAWING: Figure 4

The application discloses an artificial breeding method of Epinephelus fuscoguttatus and Plectropomus leopardus hybrids, which comprises the following steps: screening healthy Epinephelus fuscoguttatus as female parents, screening healthy Plectropomus leopardus as male parents, intensively breeding the male and female parents, the intensive breeding comprising nutrition intensification and / or temperature induction to promote gonadal maturation of the parents; collecting egg liquid of the female parents and sperm of the male parents, fertilizing to obtain fertilized eggs, incubating the fertilized eggs to obtain hybrids, and using one or more sequences in sequences SEQ ID NO. 1-8 to identify the authenticity of the hybrids, so as to determine that the hybrids inherit the genetic information of the female and male parents. The application fills the technical blank of using Plectropomus leopardus as a parent Epinephelus to carry out hybrid breeding, and makes the authenticity of the hybrids obtained by hybridization of Epinephelus fuscoguttatus and Plectropomus leopardus reliable, and the hybrids inherit the genetic information of the parents at the molecular level.

The invention relates to the technical field of feed for purple sea urchin, and discloses a preparation method of a feed formula for promoting gonad development of purple sea urchin. The method comprises the steps of performing formatting standard processing on a multi-source feed basic data set, unifying a field structure of each data source, removing structural conflict fields, and performing duplicate removal operation based on a data unique identifier to obtain an integrated feed data set; then, according to the integrated feed data set, configuring a directional optimization target including gonad development promotion optimization and nutritional ingredient balance optimization; then, according to a directional optimization target, a feed component relation map is constructed, nodes represent feed component entries, and edges represent nutrition interaction relations among the components; and finally, based on the feed component relation map and the directional optimization target, executing component optimization analysis, and generating an optimized feed formula. According to the method, the problems of multi-source data confusion, single optimization target and unclear component relationship can be solved, and a more adaptive gonad development promoting feed is provided for the purple sea urchin.

This invention discloses a method for the isolation, culture, and identification of chicken sexual gonadal cells, belonging to the field of biotechnology. The invention selects chicken embryos hatched to 18.5 days old, isolates the gonads after PCR sex identification, and uses 0.25% trypsin-EDTA digestion followed by filtration through a 70μm filter to obtain a single-cell suspension with a survival rate >90% and free from PGC contamination. A sex-specific culture system is established, allowing cells to be stably passaged and maintain a sex-specific phenotype. Through morphological, qRT-PCR, immunofluorescence, and Western blot multidimensional identification, accurate identification of male testicular Sertoli cells and female ovarian granulosa cells is achieved. This method is simple to operate, has good reproducibility, and produces high cell viability and purity. It can provide a stable gonadal microenvironment for the in vitro directed differentiation of chicken primordial germ cells, significantly improving gamete induction efficiency. It is suitable for research on avian reproductive and developmental mechanisms, transgenic breeding, and germplasm resource preservation.

The invention discloses a coreius coreius intensive cultivation method based on interspecific competition. The method comprises the following steps: S1, setting an industrial running water cultivation environment; s2, selection and stocking of coreius gigas parents; S3, domestication of the coreius gigas parents; s4, artificial feed selection and adaptation; s5, introducing bottom exogenous species such as myxocyprinus asiaticus to remove residual feed; s6, cold water slow ingestion type fishes such as acipenser schrenckii are introduced; and S7, water temperature rising control. By accurately controlling the culture environment, introducing bottom-layer species such as myxocyprinus asiaticus to remove residual feed and introducing cold water fishes such as acipenser schrenckii, the inter-species competition pressure is increased, the food snatching desire of coreius guichenoti parents is promoted, nutrition intake is increased, gonad development is promoted, meanwhile, a good ecological system is formed, the sustainability of the whole system is enhanced, and the survival rate of coreius guichenoti is increased. According to the comprehensive advantages in coreius guichenoti parent cultivation, the cultivation efficiency and economic benefits are improved, ecological balance and sustainable development can be achieved, and powerful support is provided for future aquaculture.