79 results about "Ovulation" patented technology

The invention provides an application of a CTH inhibitor and / or an xCT inhibitor in preparation of a medicine for inhibiting mammal embryo implantation, and relates to the technical field of medicines. The application effect of a cystathionine-gamma-lyase (CTH) inhibitor and / or a solute carrier family 7 member 11 (xCT) inhibitor in inhibition of mammalian embryo implantation is verified through research. The research result shows that the xCT inhibitor and / or the CTH inhibitor can obviously inhibit proliferation of corpus luteinized granular cells and secretion of progesterone of a model mouse; meanwhile, by injecting the xCT inhibitor and / or the CTH inhibitor into the mouse model in vivo, ovary ovulation and the maturation rate of the ovulated oocyte nucleus can be remarkably inhibited, functional corpus luteum formation and embryo implantation can be remarkably reduced, and then a new thought and method are provided for development of contraceptive drugs.

The invention discloses a dairy cow timed insemination method for controlling ovulation time and improving embryo implantation rate, and relates to the technical field of dairy cow breeding. The problems that follicles on ovaries of postpartum cows are not synchronous in development, ovulation time cannot be accurately regulated and controlled, and embryonic development regulation and control after fertilization are lacked are solved. The method mainly comprises the following steps: three times of gonadotropin releasing hormone injection, two times of cloprostenol sodium injection, gonadotropin releasing hormone injection, luteinizing hormone releasing hormone A3 injection, timed artificial semen deposition, and VADE mixed liquid injection. GnRH is used for actively regulating follicular development, 95% or more of the dairy cows are synchronized after 2-3 rounds of starting, then timing insemination is performed, the conception rate of the postpartum dairy cows can be remarkably increased, compared with the prior art, the pretreatment time is short, the synchronous ovulation rate and conception rate of the dairy cows can be increased, the calving interval is shortened, and the survival rate of the dairy cows is increased. The economic benefit of the pasture is obviously increased, and the breeding management level can be improved on the basis.

The invention discloses a traditional Chinese medicine composition for treating or improving hypoovarian reserve function and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following components in parts by weight: angelica sinensis, parched white paeony root, parasitic loranthus, dodder, roasted rhizoma atractylodis macrocephalae, ligusticum wallichii, herba lycopi, albizia flower and honey-fried licorice root. And after ovulation, morinda officinalis and glossy privet fruit are added. The formula provided by the invention complies with the natural law of human bodies, and according to the growth of qi and blood, yin and yang, nourishing blood, activating blood and tonifying kidney, yin and yang are tonified without stagnation, and blood stasis is removed without hurting healthy qi; meanwhile, the traditional Chinese medicine is used for strengthening the root of acquired days, quieting the main internal organs, combining dynamic and static, regulating qi-blood yin-yang balance and taking the balance as a period. The invention further provides application of the composition or the traditional Chinese medicine extract or the traditional Chinese medicine preparation in preparation of a medicine for treating or improving ovarian reserve hypofunction.

The invention discloses a pharmaceutical composition for delaying oocyte senescence and application thereof, and relates to the technical field of biological medicine, and the pharmaceutical composition comprises alpha-ketoglutaric acid, a bicarbonate buffer solution, an energy metabolism substrate, polyvinyl alcohol, hormone, a growth factor and insulin-transferrin-selenium. The pharmaceutical composition for delaying oocyte aging disclosed by the invention is beneficial to improving the maturation rate of oocytes, reducing the cytoplasm fragmentation rate and the cleavage fragmentation rate, and improving the cleavage rate and the blastocyst rate; according to the invention, the content of beta-galactosidase is reduced, the function of mitochondria in the aging process after ovulation of the mature oocyte is improved, the membrane potential of the mitochondria in the aging process after ovulation of the mature oocyte is stabilized, the activity of the mitochondria is improved, and the normalization of RNA methylation modification in the aging process after ovulation of the mature oocyte is promoted.

The invention discloses a timed insemination method for improving the pregnancy rate and the lambing rate of sheep, and belongs to the technical field of livestock breeding. In order to solve the problem that the pregnancy rate of existing sheep is unstable during regular insemination, the method comprises the steps that a progesterone sponge suppository is embedded in the vagina of a healthy and reproductive ewe on the 0th day, and meanwhile 25 g of luteinizing hormone is injected in an intramuscular mode to release hormone A3; removing the thrombus on the 12th day, and performing intramuscular injection of 400IU of pregnant mare serum gonadotropin at the same time; and after the suppository is removed, insemination is carried out on the cervix orifice of the ewe with ram semen diluted at the ratio of 1: (5-8) in 52h and 64h respectively. According to the method, ovarian activities of the ewes are regulated and controlled, the estrus synchronism and the ovulation rate are improved, tests show that the pregnancy rate reaches 81.6%, the lambing rate is 1.14, compared with a conventional method, the pregnancy rate and the lambing rate of the sheep are remarkably improved, and development of the sheep industry is promoted.

The invention relates to application of a gene inhibitor in preparation of a medicine for treating ovulation dysfunction, and belongs to the field of biomedicine.The FST content in serum of a healthy person and an OD patient is compared, it is found that the FST content in serum of the OD patient is far higher than that in serum of the healthy person, and then it is obtained that the FST content can be used as the basis for judging that the patient suffers from ovulation dysfunction. Therefore, early intervention and treatment can be carried out on patients. According to the invention, the FST high-expression monkey model is successfully constructed, and the pathogenic effect of FST high expression on the OD pathogenesis process is obtained through research of the FST high-expression monkey model; the invention further designs specific shRNA (short hairpin ribonucleic acid) to successfully inhibit the expression of FST, and after the expression of FST is inhibited, the ovarian structure of the test monkey tends to be healthy, the follicular development is normal, and the ovulation regularity is recovered, and the results show that the inhibition of the FST gene has a remarkable promotion effect on the ovarian function.

The application relates to the field of biological medicine, and particularly discloses application of a sophora japonica extract in preparation of a product for improving the reproductive performance of ewes. The sophora japonica extract can effectively inhibit pyroptosis of ovarian granulosa cells by down-regulating expression of cysteine protease B, further reverses follicle atresia and ovulation disorders caused by pyroptosis, and improves the reproductive performance; animal experiments show that the sophora japonica extract can optimize the follicle development structure of ewes, inhibit overdevelopment of large follicles, and significantly improve the ovulation efficiency, thereby providing a new scheme for inhibiting pyroptosis of ovarian granulosa cells and improving the reproductive performance of livestock.

The application discloses a superovulation method based on synergistic effect of composite hormones, which comprises the following steps: injecting PMSG and FSH to induce estrus, and injecting hCG to promote synchronous ovulation. The application adopts synergistic regulation of PMSG (long-acting follicle-stimulating hormone) and FSH (short-acting follicle-stimulating hormone), PMSG completes follicle recruitment, and FSH finely regulates the development stage, thereby avoiding overgrowth of follicles. In the examples, the Burmese cat, the British short cat and the farm cat do not appear OHSS symptoms (such as ovarian enlargement and ascites), and the ovarian state is normal.

The present application relates to the field of traditional Chinese medicine, and specifically relates to a kind of external use traditional Chinese medicine composition for treating thin endometrium, which is composed of the following raw medicinal materials: cinnamon 250-300g, fennel 250-300g, Corydalis 250-480g, Cnidium 250-400g, safflower 250-300g, Evodia 150-200g, mugwort leaf 250-300g, and ginger juice 500-600ml.The external use combined medicine of the present application can obviously improve the symptoms of dysmenorrhea, little menstrual flow, dark red menstrual color, and more menstrual blood clots, thicken the endometrial thickness during ovulation, and improve the clinical pregnancy rate of patients with thin endometrium.The external use traditional Chinese medicine is absorbed through skin, does not burden liver and kidney functions, achieves the purposes of warming meridians, activating blood and resolving stasis through the action of acupoint meridian, has little side effects, and is convenient for promotion.

1. The name of the design product: ovulation detector. 2. The use of the design product: for detecting animal ovulation. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view 1.

The invention relates to the field of animal husbandry molecular breeding and precise breeding, in particular to an animal husbandry improved variety breeding twin-fetal cattle system based on gene screening. The system comprises an ovulation potential typing module, a uterine bearing capacity quantification module, a sex compatibility verification module and a decision module. Constructing a combined evaluation model of the double-ovulation basis and the uterus metabolism capability by systematically integrating site specific amplification and gene expression quantification technologies; the core of the method is that a precise reproduction instruction is generated according to full-link data of genotypes and phenotypes; according to the method, a single ovulation rate screening mode is changed, the problem of abortion and weak abortion caused by insufficient intrauterine environment is effectively solved, and the success rate of double-fetal pregnancy is remarkably increased.

The present invention discloses a method for estimating ovulation and menstrual period based on body temperature, comprising: obtaining the user's basal body temperature just before waking up every day through a wearable device; when the number of monitoring days reaches a preset number of days, obtaining the basal body temperature of each day within the monitoring days, and traversing the user's daily basal body temperature in chronological order of the dates, recording the dates that meet the preset requirements and recording them as suspected ovulation days, and then judging whether the corresponding suspected ovulation day is an ovulation day based on the basal body temperature of each suspected ovulation day and the basal body temperature of the previous day and the basal body temperature of the following day; and determining the first day of the user's current menstrual period based on the determined ovulation day. The present invention can automatically obtain the user's body temperature and predict the date of ovulation and menstrual period, solving the problems of the prior art that require manual measurement and recording of body temperature and manual calculation of ovulation date and menstrual period, resulting in operational difficulties. The present invention also discloses an ovulation and menstrual period estimation device and storage medium based on body temperature.

The invention provides a breeding method for improving the breeding rate of replacement gilts, and the breeding method comprises the following steps: (1) adding a first functional nutritional composition into feed from 130-150 days old to the early estrus of the replacement gilts; (2) adding a second functional nutrient composition into the feed from the initial estrus period of the replacement gilts to the time before mating; and (3) within 25-35 days after mating of the replacement gilts, adding a second functional nutrient composition into the feed. According to the breeding method, oxidative stress of the replacement gilts can be relieved, development and maturation of follicles can be adjusted, oestrus and ovulation of the gilts can be promoted, the initial estrus period of the replacement gilts can be started in time, embryonic development can be promoted, the conception rate of the replacement gilts can be increased, and the replacement gilts are prevented from being eliminated due to delayed oestrus or non-oestrus or repeated breeding and infertility after oestrus; further, the breeding rate of the replacement gilts is improved.

The invention belongs to the field of medical detection, and particularly relates to a memristor for non-invasive dynamic ovulation monitoring and application. The memristor comprises a substrate, a bottom electrode, a functional layer and a top electrode which are sequentially arranged from top to bottom, wherein the functional layer is a hafnium oxide film. The memristor provided by the invention has the characteristic of ultrahigh sensitivity to electrical property changes of ion concentration and viscosity in biological fluids such as saliva, urine and the like, and a complex physiological state under regulation and control of hormones such as estrogen, LH and the like is converted into a quantifiable electric signal to be output, so that dynamic and accurate capture of a growth window is realized. The low power consumption and stable cycle characteristics of the device lay a solid foundation for developing wearable integrated equipment, and the device is expected to promote the development of reproduction management towards the family, real-time and intelligent directions, and finally the success rate and safety of assisted reproduction treatment are improved.

The invention relates to the field of fishery medicines, and discloses a megalobrama amblycephala gonadotropin releasing hormone analogue, a composite oxytocic and application. The hormone analogue MaGnRH3a provided by the invention has a remarkable effect of promoting megalobrama amblycephala egg cell maturation. The effect time of the female parent fish to be parturified by brood on the MaGnRH3a oxytocic is short, and the parturition induction efficiency is high. The modified and designed MaGnRH3a is an efficient active peptide specifically aiming at artificial spawning induction of megalobrama amblycephala. The ovulation time can be shortened to 10-13 hours and is increased by 41% compared with that of a traditional method, and the death rate of parent fishes is reduced to 0% from 16%; and through specific design, the endocrine interference risk of non-target organisms is remarkably reduced. When the active peptide and domperidone are compounded into an oxytocic, the compound oxytocic has obvious economic and ecological benefits when being applied to megalobrama amblycephala artificial breeding production.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in preparation of a sheep semen cryopreservation diluent. The invention discloses the application of alpha-ketoglutaric acid or salt thereof in the cryopreservation of the sheep semen for the first time, and the alpha-ketoglutaric acid or salt thereof can improve the semen quality of the frozen and unfrozen sheep semen and improve the fertilization ability of the frozen semen; such as sperm motility, sperm movement performance, sperm viability, sperm plasma membrane integrity rate, sperm acrosome integrity rate, sperm antioxidant capacity, sperm ATP level, sperm early apoptosis incidence rate, sperm in-vitro fertilized ovulation rate and / or artificial fertilization pregnancy rate, can be used for preparing the sheep sperm cryopreservation diluent.

The invention provides a method for promoting gonad development of mytilus coruscus, which is characterized in that estradiol is used for promoting gonad development of mytilus coruscus, and the treatment concentration of estradiol is 10-1000 [mu] g / L. Sampling analysis is carried out on the mytilus coruscus in various seasons in the Shengsi squids, the development conditions of different seasonal glands are revealed, the influence of the exogenous estradiol on the development of the gonads of the mytilus coruscus is explored, the spermiation and ovulation of parent mytilus coruscus induced by the estradiol and the fertilization and incubation conditions of larvae are observed, and the deformity rate of D-type larvae is counted. The invention not only provides a theoretical basis for biological characteristics of the mytilus coruscus in the Shengsi squids, but also reveals the important influence of the exogenous estradiol on breeding of the breeding mussels, and provides important theoretical support and practical guidance for sustainable development of the breeding industry of the mytilus coruscus.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating polycystic ovarian syndrome as well as a preparation method and application thereof. The traditional Chinese medicine composition is prepared from Chinese yam, semen cuscutae, pulp of dogwood fruit, radix glehniae, radix scrophulariae, lily, rhizoma polygonati, tortoise shell, cistanche deserticola, lotus seed and raspberry, has the effects of tonifying kidney and replenishing essence, nourishing yin and blood, replenishing qi and tonifying spleen, and benefiting uterus and accommodating fetus, and is mainly used for treating the polycystic ovarian syndrome. Research results show that the traditional Chinese medicine composition can significantly improve the sex hormone level of a patient with the polycystic ovarian syndrome, increase the endometrial thickness, improve the ovarian function and improve the endometrial receptivity, the ovulation rate and the pregnancy rate. Therefore, the traditional Chinese medicine composition has important clinical significance and wide market application prospects.

The invention discloses a composition and method for improving the ovulation concentration ratio of sows in batches and application, and belongs to the technical field of livestock breeding. The method provided by the invention comprises the following steps of: applying a composition containing taurine or a derivative thereof and lactic acid or a derivative thereof to the sow in a period from 7 to 21 days before injecting an ovulation promoting medicine to the end of hybridization, and implementing single-time timed insemination after injecting the ovulation promoting medicine buserelin. The composition can remarkably improve the ovulation concentration ratio of sows in batches, guarantee time synchronism of sperm-egg combination, further reduce the number of oestrus identification times during regular insemination, successfully realize single-time regular insemination, reduce labor, consumables, semen and boar feeding cost, and improve the breeding efficiency of the sows. And synchronous delivery is promoted, and the executability and efficiency of all-in and all-out batch production are improved.

The invention relates to the technical field of antibody preparation, particularly discloses a statin antibody and application thereof, and researches that a synthetic swine statin antigen is used for immunizing a Japanese big-ear rabbit to prepare a polyclonal antibody, and the polyclonal antibody is injected into the abdominal cavity of a mouse to promote the ovulation number of the female mouse to be obviously increased, so that the efficiency of biological purification through IVF (in-vitro fertilization) is improved. On one hand, the statin level in mice can be directly reduced; on the other hand, the ovary local microenvironment is influenced, and the superovulation level is more effectively improved. The purpose of higher superovulation is achieved by inhibiting the effect of the statin through intraperitoneal injection of the statin antibody, the efficiency is effectively improved by 50% or above, and an unexpected effect is achieved.

The invention belongs to the technical field of biological medicine, and particularly relates to application of irisin in treatment of polycystic ovarian syndrome, and the specific application of irisin in treatment of polycystic ovarian syndrome is as follows: improving the ovarian morphology and ovarian dysfunction of a patient suffering from PCOS (polycystic ovarian syndrome); the ovarian ferroptosis and iron autophagy of the PCOS patient are improved; ferroptosis of primary ovarian granular cells and KGN cells induced by high androgen is improved; iris is used for regulating and controlling an NCOA4-FTH pathway to reverse iron autophagy of ovarian granulosa cells, and through a PCOS mouse model induced by hyperandrogen, specific targets of irisin for inhibiting ferroptosis of the ovarian granulosa cells are disclosed, abnormal iron metabolism of PCOS patients is reversed, and the normal ovulation function of the PCOS patients is recovered; a safe alternative is provided: irisin can be generated endogenously by movement, avoiding the risk of metabolic disorder caused by exogenous hormone therapy.

The present invention provides an absorbent article with which the day of ovulation can be predicted in a more convenient manner than conventionally, and relates to an absorbent article, comprising a test strip for detecting luteinizing hormone from vaginal discharge using immunochromatography, wherein the test strip detects 0.05 mIU / mL to 2.5 mIU / mL of luteinizing hormone as positive.

The application discloses a culture medium for improving in-vitro aging of oocytes after ovulation and application thereof. The culture medium is prepared by adding 0.1-30 muM of astragaloside IV into an oocyte culture solution. The culture medium is safe, non-toxic and free of side effects, can reduce the oocyte fragmentation rate, active oxygen level and abnormal spindle rate of the oocytes after 24 hours of in-vitro culture, can significantly improve the mitochondrial membrane potential, in-vitro sperm binding capacity, in-vitro fertilization pronucleus rate and cleavage rate of the mature oocytes after 24 hours of in-vitro culture, and can improve the quality of the late embryos. The application of the culture medium provides favorable support for assisted reproductive technology, and has a good application prospect.

The present application relates to the technical field of antibodies, in particular to a preparation method and application of a progesterone recombinant monoclonal antibody, wherein an excellent progesterone recombinant rabbit monoclonal antibody is screened, the heavy chain variable region of the antibody has an amino acid sequence as shown in SEQ ID NO:1, and the light chain variable region of the antibody has an amino acid sequence as shown in SEQ ID NO:2. Experimental results show that the progesterone recombinant rabbit monoclonal antibody protein has a large expression amount, high specificity and high sensitivity, and can be applied to early spontaneous abortion risk assessment, prediction of adverse pregnancy, determination of ovulation, evaluation of luteal function loss and other clinical diagnoses, meanwhile, the antibody is applied to detection of progesterone content in serum, and has high accuracy, good repeatability, strong stability and good clinical application value.

The present invention discloses a system for dynamically detecting the fertile and infertile periods of women. By recording menstrual information, the system automatically marks and generates the number of days in the menstrual cycle, records and collects the basal body temperature data of the user, uses a temperature sensor to accurately measure the user's basal body temperature, and the measurement results are directly automatically entered by the system to construct a comparison database of the menstrual period and the basal body temperature. Subsequently, based on the fact that after a woman ovulates, the progesterone secreted by the ovary can cause the basal body temperature to rise. The most important thing is to determine which day is the first high-temperature period after ovulation. The method is that the system compares the basal body temperature data recorded on the current day with the highest value of the most recent 6 recorded body temperature data in the front to determine whether the body temperature today has an upward trend. Based on this theory, the detection of the infertile period after a woman ovulates is realized. The advantages of the present invention are that based on the accurate comparison of basal body temperature data, and the detection method is realized based on a temperature sensor, with fewer interference parameters, making the detection accuracy higher.

To provide a toilet device capable of easily estimating and reporting a menstruation start date or the like of a woman.SOLUTION: A toilet device 1 comprises: a toilet seat 12; a body temperature measurement sensor 14 that is disposed on a surface of the toilet seat 12 and that measures the body temperature of a person seated on the toilet seat 12; an estimation unit 182 that estimates, on the basis of the body temperature of the person measured by the body temperature measurement sensor 14, menstruation ovulation information related to menstruation and ovulation of a woman; and a transmission unit 183 that transmits, to the outside, estimated information on the menstruation ovulation information estimated by the estimation unit 182.SELECTED DRAWING: Figure 2

A method (400) and system (100) for predicting a fertility cycle of a user is disclosed A processor (302) acquires physiological biomarker data comprising at least one of a heart rate, a heart-rate variability, a skin temperature, and a skin perfusion. A rolling baseline is computed by processing the physiological biomarker data using a rolling-average technique. A stress-rhythm score that decays from a predefined value in response to non-fitness-related heart-rate elevations detected as deviations from the rolling baseline. Lifestyle-biomarker data of the user comprising at least one of a body-mass index (BMI), and an age is received. Historical fertility cycle data of the user is retrieved. A fertility cycle comprising an ovulation day and a fertile window of the user is predicted based on a weighted probabilistic analysis of the physiological biomarker data, the stress-rhythm score, the lifestyle-biomarker data, and the historical fertility cycle data of the user.

The invention relates to the technical field of traditional Chinese medicine, and discloses a traditional Chinese medicine decoction for treating polycystic ovarian syndrome and application, the traditional Chinese medicine decoction for treating polycystic ovarian syndrome is prepared by decocting a traditional Chinese medicine composition; the traditional Chinese medicine composition is prepared from radix rehmanniae preparata, cortex cinnamomi, semen brassicae, baked ginger, rhizoma curcumae longae, deer-horn glue, raw licorice roots, fried hawthorn fruits, herba leonuri, Chinese rose flowers, rhizoma chuanxiong and radix angelicae sinensis. The traditional Chinese medicine decoction for treating the polycystic ovarian syndrome has a remarkable curative effect on the symptoms of PCOS or PCOS infertility patients, and can improve the thickness and form of endometrium, regulate the phase hormone level and improve the follicle quality and the periodic ovulation rate at the same time, so that the effects of improving the clinical pregnancy rate and the total effective rate and reducing the biochemical pregnancy and fetal parturition rate are achieved; the traditional Chinese medicine decoction for treating the polycystic ovarian syndrome can improve BBT and acne of PCOS infertility patients, has no toxic or side effect, is safe and reliable, and has good clinical application value.

The invention discloses an in-vitro maturation culture medium for oocytes as well as application and a preparation method of the in-vitro maturation culture medium, and belongs to the technical field of oocyte treatment in animal breeding biotechnology. The in-vitro culture medium can delay aging of oocytes after ovulation, and the in-vitro culture medium contains ursodesoxycholic acid. Compared with a fresh group, the aging group has the advantages that the maturation rate of oocytes and the fluorescence intensity of cortical particles are remarkably reduced, the fragmentation rate, the apoptosis rate and the abnormal distribution rate of mitochondria of the oocytes are remarkably increased, and the two-cell rate, the four-cell rate and the blastocyst rate of the oocytes are remarkably reduced; the ursodesoxycholic acid group significantly improves the maturation rate and the cortical particle fluorescence intensity of the oocytes of the aged group, significantly reduces the fragmentation rate, the apoptosis rate and the mitochondrial abnormal distribution rate of the oocytes of the aged group, significantly improves the ursodesoxycholic acid of the oocytes of the aged group, and significantly improves the two-cell rate, the four-cell rate and the blastocyst rate of the aged group.

The invention discloses an acupoint application for treating polycystic ovarian syndrome and a preparation and use method thereof. The acupoint application comprises a radix salviae miltiorrhizae acupoint application, a semen cuscutae-radix salviae miltiorrhizae acupoint application, a radix morindae officinalis-radix salviae miltiorrhizae acupoint application and a semen cuscutae-radix morindae officinalis acupoint application. The traditional Chinese medicine composition is prepared from salvia miltiorrhiza, semen cuscutae and salvia miltiorrhiza, morinda officinalis and salvia miltiorrhiza, and semen cuscutae and morinda officinalis. The invention further provides a preparation method of the acupoint application and an artificial cycle therapy using method. The acupoint application medicine cake has the advantages that the acupoint application medicine cake is mainly used for treating the polycystic ovarian syndrome, has the effects of tonifying the kidney, activating blood, removing stasis and promoting follicular maturation and ovulation, is convenient and safe to apply externally, can enhance the compliance of a patient, can be absorbed through peritoneal transdermal absorption, can avoid the first-pass effect of oral administration, is closer to a disease site in the administration position, and takes effect faster.