99 results about "Ovulation" patented technology

The invention provides an application of a CTH inhibitor and / or an xCT inhibitor in preparation of a medicine for inhibiting mammal embryo implantation, and relates to the technical field of medicines. The application effect of a cystathionine-gamma-lyase (CTH) inhibitor and / or a solute carrier family 7 member 11 (xCT) inhibitor in inhibition of mammalian embryo implantation is verified through research. The research result shows that the xCT inhibitor and / or the CTH inhibitor can obviously inhibit proliferation of corpus luteinized granular cells and secretion of progesterone of a model mouse; meanwhile, by injecting the xCT inhibitor and / or the CTH inhibitor into the mouse model in vivo, ovary ovulation and the maturation rate of the ovulated oocyte nucleus can be remarkably inhibited, functional corpus luteum formation and embryo implantation can be remarkably reduced, and then a new thought and method are provided for development of contraceptive drugs.

The invention discloses a dairy cow timed insemination method for controlling ovulation time and improving embryo implantation rate, and relates to the technical field of dairy cow breeding. The problems that follicles on ovaries of postpartum cows are not synchronous in development, ovulation time cannot be accurately regulated and controlled, and embryonic development regulation and control after fertilization are lacked are solved. The method mainly comprises the following steps: three times of gonadotropin releasing hormone injection, two times of cloprostenol sodium injection, gonadotropin releasing hormone injection, luteinizing hormone releasing hormone A3 injection, timed artificial semen deposition, and VADE mixed liquid injection. GnRH is used for actively regulating follicular development, 95% or more of the dairy cows are synchronized after 2-3 rounds of starting, then timing insemination is performed, the conception rate of the postpartum dairy cows can be remarkably increased, compared with the prior art, the pretreatment time is short, the synchronous ovulation rate and conception rate of the dairy cows can be increased, the calving interval is shortened, and the survival rate of the dairy cows is increased. The economic benefit of the pasture is obviously increased, and the breeding management level can be improved on the basis.

The invention discloses a traditional Chinese medicine composition for treating or improving hypoovarian reserve function and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following components in parts by weight: angelica sinensis, parched white paeony root, parasitic loranthus, dodder, roasted rhizoma atractylodis macrocephalae, ligusticum wallichii, herba lycopi, albizia flower and honey-fried licorice root. And after ovulation, morinda officinalis and glossy privet fruit are added. The formula provided by the invention complies with the natural law of human bodies, and according to the growth of qi and blood, yin and yang, nourishing blood, activating blood and tonifying kidney, yin and yang are tonified without stagnation, and blood stasis is removed without hurting healthy qi; meanwhile, the traditional Chinese medicine is used for strengthening the root of acquired days, quieting the main internal organs, combining dynamic and static, regulating qi-blood yin-yang balance and taking the balance as a period. The invention further provides application of the composition or the traditional Chinese medicine extract or the traditional Chinese medicine preparation in preparation of a medicine for treating or improving ovarian reserve hypofunction.

The invention discloses a pharmaceutical composition for delaying oocyte senescence and application thereof, and relates to the technical field of biological medicine, and the pharmaceutical composition comprises alpha-ketoglutaric acid, a bicarbonate buffer solution, an energy metabolism substrate, polyvinyl alcohol, hormone, a growth factor and insulin-transferrin-selenium. The pharmaceutical composition for delaying oocyte aging disclosed by the invention is beneficial to improving the maturation rate of oocytes, reducing the cytoplasm fragmentation rate and the cleavage fragmentation rate, and improving the cleavage rate and the blastocyst rate; according to the invention, the content of beta-galactosidase is reduced, the function of mitochondria in the aging process after ovulation of the mature oocyte is improved, the membrane potential of the mitochondria in the aging process after ovulation of the mature oocyte is stabilized, the activity of the mitochondria is improved, and the normalization of RNA methylation modification in the aging process after ovulation of the mature oocyte is promoted.

The invention discloses an intelligent ovulation detection system and method, and the method comprises the steps: taking a picture of immune layer test paper with to-be-detected urine dropped on an immune layer of a patient, and obtaining the picture of the immune layer test paper with the to-be-detected urine dropped; receiving and processing the picture of the immune layer test paper dripped with the urine to be detected to obtain the LH concentration value of the test paper of the patient; storing test paper LH concentration values, calculated according to the immune layer test paper pictures dropped with the urine to be detected and received for multiple days continuously, of the patient for multiple days continuously; according to the change trend of the LH concentration value of the test paper of the patient for multiple consecutive days, estimating that ovulation of the patient is about to occur according to the time when the LH concentration value of the test paper is close to the peak; when it is estimated that ovulation of the patient is about to occur, the patient is prompted to conduct sex hormone examination and B-ultrasonic examination, and then it is finally confirmed that ovulation is about to occur according to the sex hormone examination result and the B-ultrasonic examination result of the patient.

The invention discloses a timed insemination method for improving the pregnancy rate and the lambing rate of sheep, and belongs to the technical field of livestock breeding. In order to solve the problem that the pregnancy rate of existing sheep is unstable during regular insemination, the method comprises the steps that a progesterone sponge suppository is embedded in the vagina of a healthy and reproductive ewe on the 0th day, and meanwhile 25 g of luteinizing hormone is injected in an intramuscular mode to release hormone A3; removing the thrombus on the 12th day, and performing intramuscular injection of 400IU of pregnant mare serum gonadotropin at the same time; and after the suppository is removed, insemination is carried out on the cervix orifice of the ewe with ram semen diluted at the ratio of 1: (5-8) in 52h and 64h respectively. According to the method, ovarian activities of the ewes are regulated and controlled, the estrus synchronism and the ovulation rate are improved, tests show that the pregnancy rate reaches 81.6%, the lambing rate is 1.14, compared with a conventional method, the pregnancy rate and the lambing rate of the sheep are remarkably improved, and development of the sheep industry is promoted.

The invention relates to application of a gene inhibitor in preparation of a medicine for treating ovulation dysfunction, and belongs to the field of biomedicine.The FST content in serum of a healthy person and an OD patient is compared, it is found that the FST content in serum of the OD patient is far higher than that in serum of the healthy person, and then it is obtained that the FST content can be used as the basis for judging that the patient suffers from ovulation dysfunction. Therefore, early intervention and treatment can be carried out on patients. According to the invention, the FST high-expression monkey model is successfully constructed, and the pathogenic effect of FST high expression on the OD pathogenesis process is obtained through research of the FST high-expression monkey model; the invention further designs specific shRNA (short hairpin ribonucleic acid) to successfully inhibit the expression of FST, and after the expression of FST is inhibited, the ovarian structure of the test monkey tends to be healthy, the follicular development is normal, and the ovulation regularity is recovered, and the results show that the inhibition of the FST gene has a remarkable promotion effect on the ovarian function.

The application relates to the field of biological medicine, and particularly discloses application of a sophora japonica extract in preparation of a product for improving the reproductive performance of ewes. The sophora japonica extract can effectively inhibit pyroptosis of ovarian granulosa cells by down-regulating expression of cysteine protease B, further reverses follicle atresia and ovulation disorders caused by pyroptosis, and improves the reproductive performance; animal experiments show that the sophora japonica extract can optimize the follicle development structure of ewes, inhibit overdevelopment of large follicles, and significantly improve the ovulation efficiency, thereby providing a new scheme for inhibiting pyroptosis of ovarian granulosa cells and improving the reproductive performance of livestock.

The invention discloses a feed and method for promoting ovaries of scatophagus argus to be mature, the feed comprises a feed 1, a feed 2 and a feed 3, after parent fish is temporarily bred in net cages, first-stage domestication is started in the net cages, the feed 1 is fed during the period, dysplastic individuals are removed after the period is finished, second-stage domestication is started in the net cages, the feed fed during the period is a mixture of the feed 1 and the feed 2, and the feed 3 is a mixture of the feed 1 and the feed 2. And after finishing feeding, rejecting the individuals with dysplasia, starting domestication at the third stage in the net cage, and feeding the feed 3 during the domestication. According to the method, the maturation of the ovary of the female fish can be remarkably promoted, the maturation of the ovary of the female fish is advanced by 2 months, synchronous sexual maturation with the male fish of the same age is achieved, the ovulation amount of the parent fish is excellent, the fertilization rate is high, and the development of the scatophagus argus artificial breeding technology can be effectively promoted.

The application discloses a superovulation method based on synergistic effect of composite hormones, which comprises the following steps: injecting PMSG and FSH to induce estrus, and injecting hCG to promote synchronous ovulation. The application adopts synergistic regulation of PMSG (long-acting follicle-stimulating hormone) and FSH (short-acting follicle-stimulating hormone), PMSG completes follicle recruitment, and FSH finely regulates the development stage, thereby avoiding overgrowth of follicles. In the examples, the Burmese cat, the British short cat and the farm cat do not appear OHSS symptoms (such as ovarian enlargement and ascites), and the ovarian state is normal.

The present application relates to the field of traditional Chinese medicine, and specifically relates to a kind of external use traditional Chinese medicine composition for treating thin endometrium, which is composed of the following raw medicinal materials: cinnamon 250-300g, fennel 250-300g, Corydalis 250-480g, Cnidium 250-400g, safflower 250-300g, Evodia 150-200g, mugwort leaf 250-300g, and ginger juice 500-600ml.The external use combined medicine of the present application can obviously improve the symptoms of dysmenorrhea, little menstrual flow, dark red menstrual color, and more menstrual blood clots, thicken the endometrial thickness during ovulation, and improve the clinical pregnancy rate of patients with thin endometrium.The external use traditional Chinese medicine is absorbed through skin, does not burden liver and kidney functions, achieves the purposes of warming meridians, activating blood and resolving stasis through the action of acupoint meridian, has little side effects, and is convenient for promotion.

1. The name of the design product: ovulation detector. 2. The use of the design product: for detecting animal ovulation. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view 1.

The invention relates to the field of animal husbandry molecular breeding and precise breeding, in particular to an animal husbandry improved variety breeding twin-fetal cattle system based on gene screening. The system comprises an ovulation potential typing module, a uterine bearing capacity quantification module, a sex compatibility verification module and a decision module. Constructing a combined evaluation model of the double-ovulation basis and the uterus metabolism capability by systematically integrating site specific amplification and gene expression quantification technologies; the core of the method is that a precise reproduction instruction is generated according to full-link data of genotypes and phenotypes; according to the method, a single ovulation rate screening mode is changed, the problem of abortion and weak abortion caused by insufficient intrauterine environment is effectively solved, and the success rate of double-fetal pregnancy is remarkably increased.

The present invention discloses a method for estimating ovulation and menstrual period based on body temperature, comprising: obtaining the user's basal body temperature just before waking up every day through a wearable device; when the number of monitoring days reaches a preset number of days, obtaining the basal body temperature of each day within the monitoring days, and traversing the user's daily basal body temperature in chronological order of the dates, recording the dates that meet the preset requirements and recording them as suspected ovulation days, and then judging whether the corresponding suspected ovulation day is an ovulation day based on the basal body temperature of each suspected ovulation day and the basal body temperature of the previous day and the basal body temperature of the following day; and determining the first day of the user's current menstrual period based on the determined ovulation day. The present invention can automatically obtain the user's body temperature and predict the date of ovulation and menstrual period, solving the problems of the prior art that require manual measurement and recording of body temperature and manual calculation of ovulation date and menstrual period, resulting in operational difficulties. The present invention also discloses an ovulation and menstrual period estimation device and storage medium based on body temperature.

The invention provides a breeding method for improving the breeding rate of replacement gilts, and the breeding method comprises the following steps: (1) adding a first functional nutritional composition into feed from 130-150 days old to the early estrus of the replacement gilts; (2) adding a second functional nutrient composition into the feed from the initial estrus period of the replacement gilts to the time before mating; and (3) within 25-35 days after mating of the replacement gilts, adding a second functional nutrient composition into the feed. According to the breeding method, oxidative stress of the replacement gilts can be relieved, development and maturation of follicles can be adjusted, oestrus and ovulation of the gilts can be promoted, the initial estrus period of the replacement gilts can be started in time, embryonic development can be promoted, the conception rate of the replacement gilts can be increased, and the replacement gilts are prevented from being eliminated due to delayed oestrus or non-oestrus or repeated breeding and infertility after oestrus; further, the breeding rate of the replacement gilts is improved.

The invention relates to application of a reagent for promoting or up-regulating the expression quantity of FOXO4 and a related medicine. The invention relates to the field of reproductive medicine, and provides application of a reagent for promoting or up-regulating the expression quantity of FOXO4 in preparation of a medicine for treating ovulation disorder of polycystic ovarian syndrome and related medicines. Aiming at an important signal channel of PCOS ovulation disorder attack, an important molecular target FOXO4 in the PCOS pathogenesis is found, it is found that feedback regulation loop imbalance between FOXO4 and AR possibly plays an important role in occurrence and development of PCOS ovulation disorder, and the FOXO4-AR can be used as a treatment target of PCOS ovulation disorder in the future, and has a good application prospect in treatment of PCOS ovulation disorder. And a new thought is provided for research and treatment methods of PCOS pathogenesis molecular mechanisms.

The invention belongs to the field of medical detection, and particularly relates to a memristor for non-invasive dynamic ovulation monitoring and application. The memristor comprises a substrate, a bottom electrode, a functional layer and a top electrode which are sequentially arranged from top to bottom, wherein the functional layer is a hafnium oxide film. The memristor provided by the invention has the characteristic of ultrahigh sensitivity to electrical property changes of ion concentration and viscosity in biological fluids such as saliva, urine and the like, and a complex physiological state under regulation and control of hormones such as estrogen, LH and the like is converted into a quantifiable electric signal to be output, so that dynamic and accurate capture of a growth window is realized. The low power consumption and stable cycle characteristics of the device lay a solid foundation for developing wearable integrated equipment, and the device is expected to promote the development of reproduction management towards the family, real-time and intelligent directions, and finally the success rate and safety of assisted reproduction treatment are improved.

The invention relates to the field of fishery medicines, and discloses a megalobrama amblycephala gonadotropin releasing hormone analogue, a composite oxytocic and application. The hormone analogue MaGnRH3a provided by the invention has a remarkable effect of promoting megalobrama amblycephala egg cell maturation. The effect time of the female parent fish to be parturified by brood on the MaGnRH3a oxytocic is short, and the parturition induction efficiency is high. The modified and designed MaGnRH3a is an efficient active peptide specifically aiming at artificial spawning induction of megalobrama amblycephala. The ovulation time can be shortened to 10-13 hours and is increased by 41% compared with that of a traditional method, and the death rate of parent fishes is reduced to 0% from 16%; and through specific design, the endocrine interference risk of non-target organisms is remarkably reduced. When the active peptide and domperidone are compounded into an oxytocic, the compound oxytocic has obvious economic and ecological benefits when being applied to megalobrama amblycephala artificial breeding production.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in preparation of a sheep semen cryopreservation diluent. The invention discloses the application of alpha-ketoglutaric acid or salt thereof in the cryopreservation of the sheep semen for the first time, and the alpha-ketoglutaric acid or salt thereof can improve the semen quality of the frozen and unfrozen sheep semen and improve the fertilization ability of the frozen semen; such as sperm motility, sperm movement performance, sperm viability, sperm plasma membrane integrity rate, sperm acrosome integrity rate, sperm antioxidant capacity, sperm ATP level, sperm early apoptosis incidence rate, sperm in-vitro fertilized ovulation rate and / or artificial fertilization pregnancy rate, can be used for preparing the sheep sperm cryopreservation diluent.

The invention relates to the technical field of condoms, in particular to a preparation method and application of a condom with an ovulation detection function, and the preparation method comprises the following steps: (1) preparing a luteinizing hormone antibody solution and an antibody carrier solution, and mixing the luteinizing hormone antibody solution and the antibody carrier solution to prepare an antibody coating solution; (2) coating the outer surface of the condom with the antibody coating solution, standing and drying to obtain an antibody coating; and (3) preparing a catalyst solution and an indicator solution, coating the antibody coating with the catalyst solution and the indicator solution, and standing and drying to obtain the condom with the ovulation detection function. According to the condom, contraception and ovulation period detection can be effectively combined, the antibody coating coated on the outer surface of the condom is combined with luteinizing hormone in female secretion, color change is generated, the ovulation period is reflected in real time, the detection sensitivity is high, and a user can make a fertility plan conveniently.

The invention provides a method for promoting gonad development of mytilus coruscus, which is characterized in that estradiol is used for promoting gonad development of mytilus coruscus, and the treatment concentration of estradiol is 10-1000 [mu] g / L. Sampling analysis is carried out on the mytilus coruscus in various seasons in the Shengsi squids, the development conditions of different seasonal glands are revealed, the influence of the exogenous estradiol on the development of the gonads of the mytilus coruscus is explored, the spermiation and ovulation of parent mytilus coruscus induced by the estradiol and the fertilization and incubation conditions of larvae are observed, and the deformity rate of D-type larvae is counted. The invention not only provides a theoretical basis for biological characteristics of the mytilus coruscus in the Shengsi squids, but also reveals the important influence of the exogenous estradiol on breeding of the breeding mussels, and provides important theoretical support and practical guidance for sustainable development of the breeding industry of the mytilus coruscus.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating polycystic ovarian syndrome as well as a preparation method and application thereof. The traditional Chinese medicine composition is prepared from Chinese yam, semen cuscutae, pulp of dogwood fruit, radix glehniae, radix scrophulariae, lily, rhizoma polygonati, tortoise shell, cistanche deserticola, lotus seed and raspberry, has the effects of tonifying kidney and replenishing essence, nourishing yin and blood, replenishing qi and tonifying spleen, and benefiting uterus and accommodating fetus, and is mainly used for treating the polycystic ovarian syndrome. Research results show that the traditional Chinese medicine composition can significantly improve the sex hormone level of a patient with the polycystic ovarian syndrome, increase the endometrial thickness, improve the ovarian function and improve the endometrial receptivity, the ovulation rate and the pregnancy rate. Therefore, the traditional Chinese medicine composition has important clinical significance and wide market application prospects.

The invention discloses a composition and method for improving the ovulation concentration ratio of sows in batches and application, and belongs to the technical field of livestock breeding. The method provided by the invention comprises the following steps of: applying a composition containing taurine or a derivative thereof and lactic acid or a derivative thereof to the sow in a period from 7 to 21 days before injecting an ovulation promoting medicine to the end of hybridization, and implementing single-time timed insemination after injecting the ovulation promoting medicine buserelin. The composition can remarkably improve the ovulation concentration ratio of sows in batches, guarantee time synchronism of sperm-egg combination, further reduce the number of oestrus identification times during regular insemination, successfully realize single-time regular insemination, reduce labor, consumables, semen and boar feeding cost, and improve the breeding efficiency of the sows. And synchronous delivery is promoted, and the executability and efficiency of all-in and all-out batch production are improved.

The invention relates to the technical field of antibody preparation, particularly discloses a statin antibody and application thereof, and researches that a synthetic swine statin antigen is used for immunizing a Japanese big-ear rabbit to prepare a polyclonal antibody, and the polyclonal antibody is injected into the abdominal cavity of a mouse to promote the ovulation number of the female mouse to be obviously increased, so that the efficiency of biological purification through IVF (in-vitro fertilization) is improved. On one hand, the statin level in mice can be directly reduced; on the other hand, the ovary local microenvironment is influenced, and the superovulation level is more effectively improved. The purpose of higher superovulation is achieved by inhibiting the effect of the statin through intraperitoneal injection of the statin antibody, the efficiency is effectively improved by 50% or above, and an unexpected effect is achieved.

The invention provides application of H151 in preparation of a product for improving an ovulation function and improving oocyte quality, and relates to the technical field of biomedicine.The H151 is supplemented to an old female mouse, it is found that the H151 can make the mood cycle of the old female mouse tend to be regularized, meanwhile, the ovulation number of the old female mouse can be remarkably increased, the number of abnormal oocytes can be reduced, and the ovulation function and the oocyte quality of the old female mouse can be improved. The ovulation function of female animals is obviously improved; meanwhile, H151 can reduce the number of spindle body morphological abnormalities of oocytes of the aged female rats, and H151 is beneficial to maintaining normal separation of chromosomes during subtraction second division of the oocytes, so that the phenomenon of chromosome dislocation during subtraction second division of the oocytes of the aged female rats is improved, and the probability of aneuploidy in the oocytes in the aging process is reduced. The invention provides powerful evidence for the H151 in preparation of products for improving ovulation function of aging female animals and improving oocyte quality.

The invention belongs to the technical field of biological medicine, and particularly relates to application of irisin in treatment of polycystic ovarian syndrome, and the specific application of irisin in treatment of polycystic ovarian syndrome is as follows: improving the ovarian morphology and ovarian dysfunction of a patient suffering from PCOS (polycystic ovarian syndrome); the ovarian ferroptosis and iron autophagy of the PCOS patient are improved; ferroptosis of primary ovarian granular cells and KGN cells induced by high androgen is improved; iris is used for regulating and controlling an NCOA4-FTH pathway to reverse iron autophagy of ovarian granulosa cells, and through a PCOS mouse model induced by hyperandrogen, specific targets of irisin for inhibiting ferroptosis of the ovarian granulosa cells are disclosed, abnormal iron metabolism of PCOS patients is reversed, and the normal ovulation function of the PCOS patients is recovered; a safe alternative is provided: irisin can be generated endogenously by movement, avoiding the risk of metabolic disorder caused by exogenous hormone therapy.

The purpose of the present invention is to provide an absorbent article and a test paper for the absorbent article in which a period suitable for natural pregnancy can be grasped at an earlier timing than before. An absorbent article according to the present disclosure has a test strip using immunochromatography for detecting luteinizing hormone (LH) from leucorrhea, the test strip being configured to estimate that a wearer of the absorbent article is in a period from five days before an ovulation day to the day of the ovulation day.

The present invention provides an absorbent article with which the day of ovulation can be predicted in a more convenient manner than conventionally, and relates to an absorbent article, comprising a test strip for detecting luteinizing hormone from vaginal discharge using immunochromatography, wherein the test strip detects 0.05 mIU / mL to 2.5 mIU / mL of luteinizing hormone as positive.

The application discloses a culture medium for improving in-vitro aging of oocytes after ovulation and application thereof. The culture medium is prepared by adding 0.1-30 muM of astragaloside IV into an oocyte culture solution. The culture medium is safe, non-toxic and free of side effects, can reduce the oocyte fragmentation rate, active oxygen level and abnormal spindle rate of the oocytes after 24 hours of in-vitro culture, can significantly improve the mitochondrial membrane potential, in-vitro sperm binding capacity, in-vitro fertilization pronucleus rate and cleavage rate of the mature oocytes after 24 hours of in-vitro culture, and can improve the quality of the late embryos. The application of the culture medium provides favorable support for assisted reproductive technology, and has a good application prospect.

The present application relates to the technical field of antibodies, in particular to a preparation method and application of a progesterone recombinant monoclonal antibody, wherein an excellent progesterone recombinant rabbit monoclonal antibody is screened, the heavy chain variable region of the antibody has an amino acid sequence as shown in SEQ ID NO:1, and the light chain variable region of the antibody has an amino acid sequence as shown in SEQ ID NO:2. Experimental results show that the progesterone recombinant rabbit monoclonal antibody protein has a large expression amount, high specificity and high sensitivity, and can be applied to early spontaneous abortion risk assessment, prediction of adverse pregnancy, determination of ovulation, evaluation of luteal function loss and other clinical diagnoses, meanwhile, the antibody is applied to detection of progesterone content in serum, and has high accuracy, good repeatability, strong stability and good clinical application value.