20 results about "Human chorionic gonadotropin" patented technology

The present application discloses a gene point mutation mouse model construction method, comprising: by taking T274M mutation on Kcnq2 Exon6 as a target, designing sgRNA and ssODN; selecting a female mouse of a set week age as an ovum donor, injecting a certain dose of serum gonadotropin and human chorionic gonadotropin into the abdominal cavity of the female mouse, then mating the female mouse with a mating male mouse, and collecting zygotes of the mated female mouse; injecting Cas9 protein, ssODN and sgRNA into the pronuclei of the zygotes by means of a microinjection technique, transplanting the zygotes obtained after injection into the ampulla of the fallopian tube of a surrogate female mouse, and obtaining a neonatal mouse after the pregnant female mouse gives birth; respectively designing a pair of identification primers F1, R1 at two ends of a mutation site on the basis of a Kcnq2 gene point mutation site, identifying the neonatal mouse, and screening out a Kcnq2T274M / + point mutant mouse in which T274M mutation is successfully knocked.

The invention relates to a gene point mutation mouse model construction method. The method comprises the following steps: designing sgRNA and ssODN by taking T274M mutation on Kcnq2Exon6 as a target spot; the method comprises the following steps: selecting a female mouse at a set week age as an ovum donor, injecting a certain dose of serum gonadotropin and human chorionic gonadotropin into the abdominal cavity of the female mouse, mating with a breeding male mouse, and collecting fertilized ova of the mated female mouse; injecting Cas9 protein, ssODN and sgRNA into the pronucleus of the fertilized ovum through a micro-injection technology, transplanting the fertilized ovum after injection into the oviduct pot belly of the female rat to be pregnant, and obtaining a newborn rat after the female rat to be pregnant is delivered; according to a Kcnq2 gene point mutation site, a pair of identification primers F1 and R1 are designed at the two ends of the mutation site respectively, and a newborn mouse is identified, so that a Kcnq2T274M / + point mutation mouse with T274M mutation successfully knocked in is screened out. The Kcnq2T274M / + point mutation mouse editing method is simple in preparation and high in repeatability, and a larger number of Kcnq2T274M / + point mutation mice can be obtained while the editing efficiency is improved.

The application provides a lateral flow chromatography test paper and application thereof. The lateral flow chromatography test paper comprises a quality control line and an absorption pad. The lateral flow chromatography test paper further comprises an intercept line arranged between the quality control line and the absorption pad, and the material of the intercept line is one or a mixture of two or more of gelatin, carrageenan, polyacrylamide hydrogel, polyacrylic acid hydrogel, polyvinyl alcohol hydrogel or polyvinyl alcohol-sodium alginate hydrogel solution. The lateral flow chromatography test paper can improve the sensitivity of commercial test paper strips, and can be applied to new crown antigen detection, human chorionic gonadotropin detection (HCG) and cardiac troponin detection. The lateral flow chromatography test paper provided by the application can significantly improve the sensitivity by adding a cheap hydrogel intercept line after the quality control line, has good universality, and can be widely applied to various colloidal gold method test paper detection and immunofluorescence test paper detection.

The present application relates to an antibody, kit, detection method, and sample analyzer for detecting thyroid stimulating hormone. Specifically, the present application relates to a monoclonal antibody or antigen-binding antibody fragment thereof that specifically binds to thyroid stimulating hormone (TSH), wherein the antibody can recognize TSH variant R55G and does not cross-react with an α-subunit shared by human luteinizing hormone (LH), follicle stimulating hormone (FSH), human chorionic gonadotropin (hCG), and free glycoprotein hormones, and is particularly suitable for developing TSH detection kit with a functional sensitivity of ≤0.0015 μIU / ml. The present application also relates to a thyroid stimulating hormone detection kit, detection method, and sample analyzer.

The present application relates to an antibody, kit, detection method, and sample analyzer for detecting thyroid stimulating hormone. Specifically, the present application relates to a monoclonal antibody or antigen-binding antibody fragment thereof that specifically binds to thyroid stimulating hormone (TSH), wherein the antibody can recognize TSH variant R55G and does not cross-react with an α-subunit shared by human luteinizing hormone (LH), follicle stimulating hormone (FSH), human chorionic gonadotropin (hCG), and free glycoprotein hormones, and is particularly suitable for developing TSH detection kit with a functional sensitivity of ≤0.0015 µIU / ml. The present application also relates to a thyroid stimulating hormone detection kit, detection method, and sample analyzer.

The utility model discloses human chorionic gonadotropin detection test paper which comprises a detector main body, main detection paper is arranged in the detector main body, an integral liquid guide sheet is arranged at the end part of the main detection paper, a liquid dropping opening is formed in the top of the liquid guide sheet, integral auxiliary detection paper is arranged on two sides of the main detection paper, and the auxiliary detection paper is arranged on the detector main body. A first display opening and a second display opening are respectively formed in the middle parts of the top ends of the auxiliary detection paper and the main detection paper. The detector is provided with the detector main body, the detection opening is formed in the front surface of the detector main body, and the sealing patch capable of being opened is arranged on the detection opening, so that the second display opening and the liquid dropping opening can be sealed in an anti-infection manner, and the safety is improved; the auxiliary detection paper is arranged on the two sides of the main detection paper, the first display opening is formed in the auxiliary detection paper, multiple times of detection can be conducted in cooperation with the main detection paper, the detection effect can be improved, and detection is more accurate.

The utility model discloses a human chorionic gonadotropin dual determination kit, which comprises a sealing cover, a kit main body, reagent paper and a urine buffer tank, the top of the kit main body is provided with the sealing cover through a hinge lug, the sealing cover can be laterally opened and controlled, the middle part of the kit main body is provided with a detection panel, and the urine buffer tank is provided with a urine buffer tank. A plurality of pieces of reagent paper are arranged on the detection panel, the reagent paper is of a replaceable structure, a urine temporary storage groove is formed in one side of the top of the kit body, and a liquid adding hopper is arranged in the urine temporary storage groove. The outer side of the kit main body is sealed with the sealing cover, the kit main body can be sealed, fixed and safely protected through the sealing cover, the handle frame is arranged in the middle of the top end of the sealing cover, and the kit main body can be conveniently carried by hand through the handle frame; a detection panel is arranged in the middle of the kit main body, a plurality of pieces of reagent paper are arranged on the detection panel, and display ports are formed in the reagent paper.

The invention provides application of HCG18 in preparation of a medicine for treating liver cancer by regulating hepatoma ferroptosis. The HCG18 silently promotes accumulation of lipid ROS and Fe < 2 + > in cells, reduces the content of GSH in the cells and enhances cell death induced by erastin at the same time, overexpression of the HCG18 causes remarkable reduction of lipid ROS and Fe < 2 + >, increases the concentration of GSH in the cells and reduces cell death induced by erastin at the same time, and the HCG18 is a competitive binding agent of miR-30a-5p and promotes expression of RRM2 through competitive binding of miR-30a-5p.

1. Name of the Design Product: Saliva Detection Pen (HCG). 2. Use of the Design Product: A detection device for collecting saliva to detect the value of human chorionic gonadotropin, thereby determining whether a person is pregnant. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective view.