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1 results about "Human chorionic gonadotropin" patented technology
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Human chorionic gonadotropin (hCG) is a hormone produced by the placenta after implantation. The presence of hCG is detected in some pregnancy tests (HCG pregnancy strip tests). Some cancerous tumors produce this hormone; therefore, elevated levels measured when the patient is not pregnant may lead to a cancer diagnosis and, if high enough, paraneoplastic syndromes, however, it is not known whether this production is a contributing cause, or an effect of carcinogenesis. The pituitary analog of hCG, known as luteinizing hormone (LH), is produced in the pituitary gland of males and females of all ages.
A method for extracting monophasic menotropin from human urine
This invention relates to the field of biopharmaceutical technology, and particularly to a method for extracting unimodal human chorionic gonadotropin (hCG) from human urine. The method includes: loading urine from a healthy pregnant woman onto a YKW214 resin chromatography column, treating it with a washing buffer and eluent containing sodium chloride, and collecting the eluent; ultrafiltration and desalting to obtain a first ultrafiltrate; adjusting the pH and loading it onto a monoclonal antibody chromatography column with a ligand specifically binding to an HCG β subunit antibody, washing and eluting, and collecting the eluent; ultrafiltration and desalting again to obtain a second ultrafiltrate; performing virus-free filtration; adjusting the pH and loading it onto an RPC15 chromatography column, selectively removing HCG containing only the β subunit by utilizing the difference in the number of hydrophobic amino acids between intact HCG molecules and free β subunits, collecting the eluent; adding ethanol for precipitation, centrifuging, and vacuum drying to obtain unimodal hCG. This method effectively removes impurities such as free β subunits, resulting in a product with high purity, high yield, good safety, simple operation, and suitability for large-scale production.
Owner:HENAN MEDSCIENCE PHARM CO LTD