104 results about "Chorionic gonadotrophin" patented technology

Use of a growth hormone protein and polynucleotides encoding same comprising an amino-terminal carboxy-terminal peptide (CTP) of chorionic gonadotrophin and two carboxy-terminal chorionic gonadotrophin CTPs attached to the growth hormone in methods of inducing growth or weight gain, method of increasing insulin-like growth factor (IGF-1) levels, and methods of reducing the dosing frequency of a growth hormone in a human subject are disclosed. Pharmaceutical compositions comprising the growth hormone and polynucleotides encoding the growth hormone of the invention and methods of using same are also disclosed.

Polypeptides and polynucleotides encoding same comprising at least one carboxy-terminal peptide (CTP) of chorionic gonadotrophin attached to a carboxy terminus of a coagulation factor and not to an amino terminus are disclosed. Pharmaceutical compositions comprising the polypeptides and polynucleotides of the invention and methods of using same are also disclosed.

A polypeptide and polynucleotides comprising at least two carboxy-terminal peptides (CTP) of chorionic gonadotrophin attached to a non-human peptide-of-interest are disclosed. Pharmaceutical compositions comprising the non-human polypeptides and polynucleotides of the invention and methods of using both human and non-human polypeptides and polynucleotides are also disclosed.

A polypeptide and polynucleotides encoding same comprising one carboxy-terminal peptide (CTP) of chorionic gonadotrophin attached to an amino terminus of a growth hormone and two carboxy-terminal peptides (CTP) of chorionic gonadotrophin attached to a carboxy terminus of a growth hormone are disclosed. Pharmaceutical compositions comprising the polypeptide and polynucleotides of the invention and methods of using same are also disclosed.

A polypeptide and polynucleotides encoding same comprising one carboxy-terminal peptide (CTP) of chorionic gonadotrophin attached to an amino terminus of a cytokine and two carboxy-terminal peptides (CTP) of chorionic gonadotrophin attached to a carboxy terminus of a cytokine are disclosed. Pharmaceutical compositions comprising the polypeptide and polynucleotides of the invention and methods of using same are also disclosed.

A polypeptide and polynucleotides comprising at least two carboxy-terminal peptides (CTP) of chorionic gonadotrophin attached to a non-human peptide-of-interest are disclosed. Pharmaceutical compositions comprising the non-human polypeptides and polynucleotides of the invention and methods of using both human and non-human polypeptides and polynucleotides are also disclosed.

A polypeptide and polynucleotides comprising at least two carboxy-terminal peptides (CTP) of chorionic gonadotrophin attached to a non-human peptide-of-interest are disclosed. Pharmaceutical compositions comprising the non-human polypeptides and polynucleotides of the invention and methods of using both human and non-human polypeptides and polynucleotides are also disclosed.

The invention relates to the medical field of immunoassay, and concretely provides a dissociate human chorionic gonadotrophin beta-subunit magnetic particle chemiluminescence quantitative assay kit having the advantages of simplicity, rapidness, high sensitivity, wide linear range and good stability, and its preparation method. The method combines a magnetic particle immune separating technology to apply an enzymatic chemiluminescence substrate on the basis of enzyme-linked immunosorbent assay, and allows an optical signal generated by the detection of the luminescence substrate to substitute a chromogenic substrate in enzyme immune assay, so the method has the advantages of substantially improved sensitivity, simple operation and wide practicality, can be applied to open semi-automatic chemiluminescence detectors, can also be applied to full-automatic measure systems, and can realize batch and fast detection, low use cost, and easy popularization and application.

The invention belongs to the field of animal husbandry, and particularly relates to a method for promoting female and male parent giant salamanders to synchronously mature. The method is suitable for artificial rearing of giant salamanders. The method is characterized by being realized through the following mode: firstly, selecting sexually-mature parent giant salamanders, and artificially domesticating the parent giant salamanders to enable the same to be capable of taking in food initiatively; and secondly, feeding spawning-inducing medicine to the artificially-domesticated parent giant salamanders from the middle of August to the beginning of September in each year, wherein the spawning-inducing medicine is prepared by mixing luteinizing hormone releasing hormone analogs (LHR-A), human chorionic gonadotrophin (HCG) for fishes and carp pituitary glands. The method of feeding the spawning-inducing medicine is adopted to promote sexual glands of the parent giant salamanders to mature, so that sperm producing amount of male parent giant salamanders is increased by at least 35%, sperm activity is improved by more than 40%, egg laying amount of female parent giant salamanders is increased, and success rate of artificial fertilization is increased by more than 85%.

A method of determining the estimated date of implantation of an embryo may include testing a sample of a body fluid from a pregnant human female subject so as to obtain data indicative of the concentration of human chorionic gonadotrophin (hCG) in the sample, and calculating an estimated date of implantation from the hCG concentration data.

The invention relates to hyperglycosylated-human chorionic gonadotrophin (H-HCG) rapid immune diagnosis chromatography test paper, characterized by comprising a sample pad, a nitrocellulose membrane enveloped with a detection line T and a quality control line C, and a sample absorption pad which are sequentially connected, wherein the sample pad, the nitrocellulose membrane and the sample absorption pad are stuck on a support plate. The H-HCG rapid immune diagnosis chromatography test paper has the advantages that (1) due to the design that a conjugate pad and the nitrocellulose membrane are separately enveloped with an H-HCG specific antibody B207 or B152, an antibody for resisting alpha-HCG or beta-HCG, or an antibody for resisting intact HCG, by detecting the embodiments such as blood, urine, saliva and other samples, of H-HCG content of a human body, whether a person to be tested is pregnant or not and whether the placental function of a pregnant woman is good or not can be known, abortion can be predicted, and the like; (2) the instant diagnostic reagent use an immune chromatography test paper method for rapid detectiong, which is suitable for diagnosis and differential diagnosis of normal first trimester pregnancy and abnormal first trimester pregnancy, and has the advantages of being high in specificity, high in sensibility, safe, easy to store, good in stability, convenient in application, and the like.

Methods are provided for the establishment and maintenance in long term culture of hormone secreting cells. Cells are derived from tumorous or non-tumorous animal or human tissues, including ovary, endometrium, trophoblast, pituitary, thyroid, and pancreas. The cells secrete into the culture medium hormones such as estrogens, progestins, follicle-stimulating hormone, luteinizing hormone, human chorionic gonadotrophin, thyroxin, glucagon, and insulin, depending on the tissue of origin of individual cell cultures. Contact with an appropriate secretogogue causes the cells to respond with increased hormone secretion. For instance, ovarian follicular cells respond to follicle-stimulating hormone with increased estrogen and progesterone secretion. Pancreatic cells respond to elevated glucose with increased insulin secretion. The cells proliferate in in vitro for up to one year or longer, during which time they retain their hormone-secretion profile. The cells may be frozen for storage, and retain their hormone-secretion profile after thawing. The cell cultures are useful for the production of human hormones, for the bio-assay of drugs such as therapeutic gonadotrophin, for the testing of drug efficacy and design, and for toxicity testing of drugs and chemicals. The cells may also be implanted in an individual to replace deficient hormone secretion. For instance, insulin secreting pancreatic cells may be implanted in a diabetic individual as an adjunct or replacement therapy for exogenously administered insulin.

The invention provides an artificial breeding method for silurus meridionalis. The method includes the steps of selecting parent fish, cultivating the parent fish and performing artificial spawning induction, fertilization and hatch, wherein the weight of the selected parent fish ranges from 8 kg to 10 kg, the selected parent fish is more than three years old and is healthy and free of disease, the parent fish is introduced into a pond with the area being 120 square meter and water depth being 1.5-2.0 m to be raised, oxytocin is chorionic gonadotrophin and carp pituitary gland, water temperature for spawning induction is 25.5-29.8 DEG C, the effect time lasts for 10-12 hours, artificial dry fertilization is performed with the female-male ratio being 2:1, a hatching pond is 3.8 m long, 3.1 m wide and 1.2 m high, and water depth is 0.4 m; strict disinfection is performed in the mode that 0.3 kg of quicklime is spread on each square meter of the hatching pond; after toxicity disappears, water for hatching is placed in the hatching pond, and polyethylene net sheets with fertilized eggs are directly placed into the hatching pond for lentic hatching. The method is convenient to use, and the hatching rate can be increased by 15% when the silurus meridionalis is bred.

The invention discloses an artificial-inducing mixing agent and artificial breeding method for largemouth bass, and belongs to the technical field of aquatic animal breeding. The provided mixing agentincludes chorionic gonadotropin, luteinizing hormone releasing hormone analogue No.2 and DOM. Through the adoption of the mixing agent to artificially breed largemouth bass, bred females can have high brood amount, high egg maturity can be achieved, fertilization rate and hatchability can be significantly increased, the breeding efficiency of the largemouth bass can be effectively enhanced, and time can be saved.

The invention discloses a beta-human chorionic gonadotrophin test kit (time-resolved fluoroimmunoassay) applicable for early-pregnancy and mid-pregnancy prenatal screening and a preparation method thereof. The kit comprises the following main components of an experimental buffer solution, a concentrated washing solution, an enhancement solution, a reaction plate, a standard product and a quality control product of filter paper dried blood spots and a europium marker. The method for preparing the kit according to the invention comprises the following steps of: 1. preparing the experimental buffer solution, the concentrated washing solution and the enhancement solution; 2. coating the reaction plate; 3. preparing the standard product and the quality control product; 4. preparing the europium marker; 5. separately loading; 6. sticking labels; and 7. assembling into a finished product. The invention has the characteristics that the accuracy and the sensitivity of a detected result are high, the stability is good, and a detecting method is economical, convenient and safe and has no traumatic property and the like. A filter paper dried blood spot technology is applied to prenatal screening work, which is beneficial to blood specimen collection, storage and transportation and ensures the experimental reliability.

The invention relates to a detection kit for hyperglycosylated modification of an hCG (human chorionic gonadotropin) tumor marker. According to the detection kit, magnetic beads are combined with an antibody (MCA-A) to serve as a capture antibody to capture total hCG in a to-be-detected sample, wherein the affinity of the antibody (MCA-A) is least influenced by glycosylation change; an antibody (MCA-B) most influenced by glycosylation change and another antibody (MCA-C) less influenced by glycosylation are used for detecting the total protein content and the N-glycosylation modification degree of hCG in the sample simultaneously, so that a tumor biomarker is identified. The detection kit improves the accuracy, the sensitivity and the specificity of an immunodiagnosis method greatly and has great practical value for detection of hyperglycosylated modification of the tumor marker in the actual sample.

The invention discloses a pig oocyte in-vitro maturation culture solution and a preparation method and application thereof, and belongs to the technical field of oocyte in-vitro maturation culture. The problems of low in-vitro maturation rate and development rate of porcine oocytes currently are solved. The pig oocyte in-vitro maturation culture solution is prepared from a basal culture solution TCM-199, penicillin, streptomycin, NaHCO3, 4-hydroxyethylpiperazine ethane sulfonic acid, polyvinyl alcohol, sodium pyruvate, insulin, cysteine, epidermal growth factor, porcine follicular fluid, pregnant mare serum gonadotropin, human chorionic gonadotropin and tannic acid. According to the pig oocyte in vitro maturation culture solution, the oocyte in-vitro maturation quality, the cumulus cell diffusion index, the parthenogenetic embryo blastocyst rate and blastocyst total cell number, and the in-vitro fertilization embryo cleavage rate, blastocyst rate and blastocyst total cell number can beimproved.

The invention relates to a kit for the mass spectrometry antibody of human chorionic gonadotropin isomers and a preparation method thereof, which pertain to the technical field of protein detection. The kit comprises a tubelet of mass spectrometry standard quality control serum containing a U9 buffer solution, a tubelet of magnetic beads containing equivalent 1Mug of anti hCG, anti hCGBetacf, anti hCG-Alpha and anti hCG-Beta, a tubelet of buffer solution, a tubelet of eluent and a tubelet of energy absorption molecular saturated solution and all the tubelets are put into a cold storage box at the temperature of 4 DGE C. The invention can be applied to mass spectrometry antibody kits of in vitro sample detection and prognosis judgement. The method is accurate, convenient and fast.