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59 results about "Pituitary gland" patented technology

In vertebrate anatomy, the pituitary gland, or hypophysis, is an endocrine gland about the size of a pea and weighing 0.5 grams (0.018 oz) in humans. It is a protrusion off the bottom of the hypothalamus at the base of the brain. The hypophysis rests upon the hypophysial fossa of the sphenoid bone in the center of the middle cranial fossa and is surrounded by a small bony cavity (sella turcica) covered by a dural fold (diaphragma sellae). The anterior pituitary (or adenohypophysis) is a lobe of the gland that regulates several physiological processes (including stress, growth, reproduction, and lactation). The intermediate lobe synthesizes and secretes melanocyte-stimulating hormone. The posterior pituitary (or neurohypophysis) is a lobe of the gland that is functionally connected to the hypothalamus by the median eminence via a small tube called the pituitary stalk (also called the infundibular stalk or the infundibulum).

A kind of breeding method of sex reversal of female carp into male carp

The invention discloses a culturing method for sex reversal of a female carp to a male carp. The culturing method comprises the following steps that A. squeezing an abdomen of a 10-11-month-old carp, and separating a male carp with out-flown semen and a carp with no out-flown semen; B. inserting a copper roe digging device in a genital pore of the carp from which semen can not be squeezed, and selecting the carp capable of obtaining roe particles to cultivate the carp in a cement pond; C. according to the weight of the carp, feeding the carp with dosing fodders containing 17 alpha- methyltestosterone every day, and mix-injecting domperidone, luteinizing hormone releasing hormone and carp pituitary gland in the thoracic cavity of the carp in each five days; and D. after continuously processing the test carp for 2 months, squeezing the abdomen of the carp, the test carp with out-flown milky white semen is the sex reversal carp which is a female on genotype and a male on physiology. The culturing method is easy and feasible and is convenient in operation, and can be used for sex reversing the female carp on genotype into a male carp on physiology. The growth speed of the filial generation cross bred by the sex reversal carp and the common carp is fast and the economic benefits are remarkable.
Owner:INST OF AQUATIC LIFE ACAD SINICA

Artificial eel propagation method

InactiveCN102511424ALower dose of steroid injectionsOptimize the selection processClimate change adaptationPisciculture and aquariaAnimal sciencePituitary gland
The invention relates to an artificial eel propagation method, which includes: a, ripening parent fishes, breeding catadromous parent reels for seven days, gradually increasing salinity of water, and subjecting the parent eels to back intramuscular injection of ripening agent-carp pituitary gland homogenate and HCG (human chorionic gonadotropin); b, performing induced spawning of the parent eels, subjecting female eels to injection of oxytocin 17 alpha-hydroxyl progesterone and HCG, subjecting sperm-producing male eels to injection of mixture of CPE (carp pituitary extract) with HCG, collecting naturally spawned fertilized eggs on the next day, and subjecting the female eels laying no eggs to semi-dry artificial insemination; and c, hatching the fertilized eggs, immediately transferring the fertilized eggs to a special-made hatching barrel for hatching, and slightly aerating, changing half of water every four hours until fry are hatched successfully. The artificial eel propagation method has the advantages that anesthetic loss and operational injury can be prevented, injection dosage of hormones is reduced, a great amount of eels can mate and lay eggs naturally under artificial induced spawning, artificial insemination can be complete securely in a planned way, level of high-quality fertilized eggs and alevin of eels artificially propagated is increased.
Owner:SHANGHAI OCEAN UNIV

Chinese herbal medicine heightening insole

The invention discloses a Chinese herbal medicine heightening insole which is composed of an insole body and a Chinese herbal medicine acupoint plaster, wherein the Chinese herbal medicine acupoint plaster is obtained by preparing medicine bags from Chinese herbal medicine preparations and applying the medicine bags on positions on the insole corresponding to acupoints and reflecting regions of a vola. Bulk drugs for the medicine bags in the heightening insole comprise manchurian wildginger, borneol, safflower, clematis root, dahurian angelica root, dragon's blood, frankincense, myrrh, lightyellow sophora root, Sichuan lovage rhizome, peony root, common bletilla, Chinese angelica, amur cork-tree bark, argy worwood and common cnidium fruit. The Chinese herbal medicine heightening insole provided by the invention has a simple structure, can stimulate acupoints at reflecting regions of a vola, stimulates secretion of human growth hormone, accelerates division of bone cells, promotes growth of bones, is applicable to short teenagers and exerts effects on sterilization, invigoration of the circulation of blood, recovery from fatigue, deodorizing, absorption of sweat and the like. The Chinese herbal medicine bags used in the invention are easily and conveniently prepared, can stimulate reflecting regions of pituitary gland, Yongquan aperture and a sleeping aperture at both feet and can effectively promote secretion and release of growth hormone by anterior pituitary, thereby promoting growth of bones of a body. Moreover, raw materials for the insole are cheap, a preparation method for the insole is simple and can be widely applied in production, so good social values and economic benefits are obtained.
Owner:杨高林

Method for separating and culturing pituitary gland cells of rat

The invention provides a method for separating and culturing pituitary gland cells of a rat. The method comprises steps as follows: (1), an 8d male SD rat is selected and subjected to anesthesia execution; (2), the anesthetized rat is fixed on a board with the ventral side facing upwards, the pituitary gland of the rat is taken out under the sterile condition, put in a pre-cooled PBS (poly(butylene succinate)) liquid and washed repeatedly, bloodstain is removed, and pituitary gland envelope is removed; (3), the pituitary gland is cut into 1 mm*1 mm fragments by ophthalmic scissors in the pre-cooled PBS liquid under the sterile condition; (4), pre-heated III type collagenase is combined with neutral enzyme for mixed digestion; (5), the digestion is stopped, filtering is performed, a filtrate is collected and centrifuged, a supernatant is abandoned, a culture medium is added for resuspension, and the operation is repeated three times; (6), a 24-hole culture plate is inoculated with cells for culture; (7), the cells cultured for 7d are subjected to trypsin digestion and then are purified by a screen again, a supernatant is abandoned after centrifugation, the cells are resuspended, and the treated culture plate is inoculated with the cells; (8), the cells are subjected to cell immunofluorescence identification. The method for separating and culturing the pituitary gland cells of the rat is simple to operate, the obtained pituitary gland cells are high in survival rate and good in activity, can be used for establishing a cell experimental model in vitro and meet the requirement of pituitary gland cell experiments.
Owner:JIANGYIN CHI SCI

Beluga artificial propagation method and application of carp pituitary gland

The invention relates to the field of beluga culture, particularly discloses a beluga artificial propagation method and application of a carp pituitary gland, and relates to a beluga artificial propagation method. The method comprises the steps that 1, nutrient enrichment culture is performed on a beluga milter parent and a beluga raun parent; 2, the beluga milter parent and the beluga raun parent obtained after nutrient enrichment culture are subjected to low temperature stimulation; 3, the water temperature is increased to the pre-spawning induction temperature, and spawning induction medicine administration is performed on the beluga milter parent and the beluga raun parent so as to be subjected to spawning induction; 4, free eggs and seminal fluid are collected to be subjected to artificial fertilization and incubation, wherein the spawning induction medicine is the carp pituitary gland. The invention further relates to application of the carp pituitary gland for beluga artificial propagation, and application of the carp pituitary gland for preparing medicine for artificial propagation of beluga. The beluga spawning induction medicine is easy to obtain and prepare, the spawning induction rate of spawning induction propagation performed by adopting the beluga spawning induction medicine is high, the fertilization incubation effect is good, and the postpartum parent fish survival condition is good.
Owner:BEIJING FISHERMAN AQUATIC PROD TECH DEV CENT +1
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