691 results about "Tilapia" patented technology

The invention relates to a general-purpose composite Chinese herbal medicine immune enhancer for freshwater cultured fish, which is made of raw materials with the following weight ratios, based on the amount added per ton of feed: rhubarb 800-2000g; Sanqi 350-1000g; water Silybum 1200-2500g; Cassia 600-800g; Astragalus 600-2000g; Scutellaria baicalensis 500-900g; 500-1500g; Garlic 1000-2000g. After many tests in the laboratory, it has been proved that the general-purpose compound Chinese herbal medicine immune enhancer for freshwater aquaculture fish can improve the survival rate of a variety of freshwater aquaculture fish in the process of attacking viruses; The long-term breeding experiment of farmed fish feed proves that the general-purpose compound Chinese herbal medicine immune enhancer for freshwater farmed fish can effectively reduce the incidence of a variety of high-density farmed freshwater fish and improve the survival of the breed; the additive is safe, efficient, and has no side effects for long-term use. The breeding objects have strong vitality, rapid growth, bright body color, high feed conversion rate, and good breeding benefits.

The invention relates to a tilapia mossambica feed, in particular to an artificial compound feed for tilapia mossambica. An artificial compound feed with function of preventing tilapia mossambica streptococcicosis comprises the following components in percentage by mass: 5 to 10 percent of fish meal, 20 to 30 percent of bean pulp, 20 to 30 percent of rape pulp, 10 to 15 percent of cotton pulp, 10 to 15 percent of DDGS (maize alcohol lees and residual liquid dry substance), 5 to 10 percent of rice bran, 15 to 20 percent of flour, 5 to 10 percent of maize, 1 to 2 percent of soybean oil, 0.5 to 1.5 percent of phospholipid oil, 1 to 2 percent of calcium hydrogen phosphate, 1 to 10 percent of bentonite, 0.5 to 1 percent of multi-dimensional multi-mineral premix, 0.1 to 0.5 percent of choline chloride, 0.01 to 0.05 percent of antioxidant, 0.1 to 0.5 percent of mildew-proof agent and 1 to 5 percent of composite Chinese medicinal additive. Multiple antibacterial Chinese medicinal herb additives are added into the feed, so the feed improves the antibacterial capability of the tilapia mossambica and can prevent the tilapia mossambica streptococcicosis.

The invention relates to an ecological breeding method for mixedly breeding tilapia mossambica and litopenaeus vannamei boon, which is used for breeding fish and prawn in a pond. The invention discloses an ecological breeding mode for mixedly breeding the tilapia mossambica and the litopenaeus vannamei boon. In every spring (from late March to early April), when water temperature stably recoveries at more than 20 DEG C, the pond can be sterilized by calcium oxide in a drying way with quantity being 75-100 kilogram for each acre, after 5-7 days when toxicity is unavailable, the litopenaeus vannamei boon larvaes and the tilapia mossambica seeds are stocked by means of mixed breeding, wherein the litopenaeus vannamei boon is stocked at least for one time. Feeding quantity in each day is depended on 3-5% of the weight of the tilapia mossambica twice a day, and can be properly increased or decreased according to weather, water quality and feeding condition without special feed for the litopenaeus vannamei boon, the pond is sterilized by calcium oxide with quantity being 15-20 kilogram for each acre, once to twice in each month, and the pH value of the pond is adjusted to be alkalescence. The method can naturally obtain the best ecological benefit of cultural environment, reduces disease of fish and prawn, increases physique, improves growth speed, enlarges the individual size, and increases average yield.

The invention discloses a method for producing fermentation bean pulp rich in feeding use functional peptides. The present invention relates to a manufacturing technique taking the dehulled bean pulp as raw materials, using the microbial fermentation technology to remove various nutrilit-resistance in the bean pulp and simultaneously obtaining a great deal of polypeptide fermentation bean pulps, specifically to a microbial fermentation process using multi-strain mixed solid fermentation for preparing bean pulp rich in feeding use functional peptides. The manufacturing technique including mixing uniformly a fermentation nutrient fluid composed of three strains of expansively trained Bacillus subtilis, one strain of Saccharomyces crevisiae and one strain of Lactobacillus according to the proportion 1.8:1:1; inoculating 5% of the inoculated amount into the cottonseed pulp the water content of 50% ( sieving with a 40 mesh sieve), simultaneously adding 5% of molasses, and mixing uniformly (with initial pH value of 7.0); aerobic fermentation at temperature of 40 DEG C for 48 hours, and then performing dry sampling at 60 DEG C, and finally performing nutrition component analysis to the bean pulp fore and after fermentation. The completed product of the fermentation bean pulp can be stored in a cool dry place for 6-9 months after weighing and packaging, which can be the feeds of pig, chicken, tilapia and other animals.

The present invention discloses a kind of frozen tilapia mossambica fish fillets containing freezing protective agent trehalose and its processing method. Said processing method includes the following steps: making the tilapia mossambica fish undergo the processes of cleaning dressing, boning, skinning and filleting treatments, then adding protective agent to treat fish fillets, taking out said fish fillets, draining to remove excess solution and freezing below-18 deg.C so as to obtain the invented frozen tilapia mossambica fish fillets. The described protective agent is an aqueous solution containing trehalose, glycine and sodium chloride.

The invention relates to a type of feed, in particular to a type of compound feed of tilapia. The compound feed of tilapia comprises the following raw materials according to mass percent: 3-8% of fish meal, 20-30% of bean pulp, 5-10% of peanut pulp, 10-15% of corn, 8-12% of cottonseed pulp, 15-20% of rape seed pulp, 12-18% of strong flour, 1-2% of monocalcium phosphate, 1-2% of composite mineral substance, 1-2% of composite vitamins and 1-2% of soybean oil. The feed of tilapia in the invention has the advantages of comprehensive nutrition, promoting growth, strengthening immunity, resisting stress, protecting the intestinal canal of the fish body, preventing occurrence of diseases and the like.

The invention provides bacillus velezensis and application of the bacillus velezensis as an aquatic pathogenic bacteria inhibitor. The bacillus velezensis is separated from the intestinal canal of healthy tilapia, and the preservation number of the bacterial strain is GDMCC No. 60344. The bacillus velezensis has a broad-spectrum antagonistic aquatic common pathogenic bacteria function, and has theadvantages that the growth of the pathogenic bacteria, such as streptococcus agalactiae, streptococcus iniae,nocardia seriolea, aeromonas hydrophila, aeromonas schubertii and edwardsiella tarda, canbe effectively inhibited, the use is safe, no residue is generated, reducing the usage amount and the residual amount of chemical drugs in aquaculture is facilitated, the production cost is reduced, and the practicality is strong.

The invention provides a collagen and a collagen polypeptide, wherein, the weight percentage of hydroxylysine is more than 1.3 percent. The invention also provides the preparation technology and the application of the collagen and the collagen polypeptide. The invention can make full use of inexpensive raw materials including the fish skin and fish scale of tilapias to produce products with high added value. The collagen polypeptide is used for treating arthritis with a definite drug effect, and the clinical dose is considerably less than other collagen polypeptides. The collagen polypeptide can solve the pollution problem of fish skin wastes, and at the same time avoid the risk of infectious diseases which can be caused by the collagen of land animals. The collagen and the collagen polypeptide have the advantages of considerable economic benefits, energy conservation and environmental protection, simple process equipment, low cost and easy industrialization.

The invention discloses a processing method of dried fish leisure food. In the method, the fish without intermuscular bones such as tilapia or Spanish mackerel with skin is used as a raw material which is subjected to slicing, rinsing, pickling, baking, frying, seasoning and the like to obtain the dried fish leisure food meeting the requirements for the modern healthy food. Compared with the traditional method, the method disclosed by the invention reduces the use of chemical agents, reduces the grease content of the product, realizes good taste and conforms to the modern food consumption concept concerning environmental protection, health and safety.

The invention discloses a compound feed for preventing fatty liver of tilapia and a preparation method of the feed. The compound feed is prepared from the following raw materials according to a certain proportion: fish meal, fermented soybean meal, meat and bone meal, rapeseed meal, cottonseed meal, wheat bran, wheat flour, calcium dihydrogen phosphate, multi-vitamin premix, multi-mineral premix, soybean oil, soybean lecithin, chitosan oligosaccharide, astragalus polysaccharide and choline chloride. The preparation comprises the following steps of: respectively crushing and screening all the raw materials (except the soybean oil) by a crushing machine, weighing the flour, the calcium dihydrogen phosphate, the multi-vitamin premix, the multi-mineral premix, the soybean lecithin, the chitosan oligosaccharide, the astragalus polysaccharide, the choline chloride and other raw materials according to the mass fraction in the formula and mixing uniformly, further weighing the remaining raw materials, uniformly stirring and mixing, fully uniformly mixing with water, performing extrusion and pelletization by a feed machine, and drying at the temperature of 55-60 DEG C to prepare the feed for the tilapia. The compound feed has balanced nutrition, is suitable for requirements of healthy growth of the tilapia and can further reduce the accumulation of fat in livers of the tilapia and improve disease resistance of the tilapia.

The invention discloses a fermented low-salt semi-dried tilapia mossambica, a processing method thereof and application thereof. The processing method comprises the steps of: removing the internal organs and the scales of the tilapia mossambica; cutting along backside or abdomen; overturning; flatly pressing to form a symmetrical tilapia mossambica piece except the tail of the tilapia mossambica;washing with cleaning water; washing with salt water with 1%-4% of concentration to remove fishy smell and obtain the tilapia mossambica piece without the fishy smell; paving salt on the surface of the tilapia mossambica piece without the fishy smell, wherein the weight of the salt is equal to 3-8% of that of the tilapia mossambica; sousing for 8-24 hours; drying; or soaking for 8-24 hours with 5-10% of salt water to obtain the low-salt tilapia mossambica piece; spraying zymophyte on the surface of the tilapia mossambica piece, wherein the inoculum size of the zymophyte is 105-107 CFU / g fish meat; fermenting for 10-48 hours in a sealing way under the temperature of 12-20 DEG C; and drying the fermented tilapia mossambica piece to obtain the fermented low-salt semi-dried tilapia mossambicawith 30-60% of water content. The product has lubricating meat quality and typical flavor of dried meat. The processing method can be adapted to processing freshwater fishes.

The invention provides a preparation method of crisp Tilapia middle vertebrae leisure food. The Tilapia middle vertebrae is adopted as the raw material, the raw material is undertaken the high-temperature acidizing cooking treatment after the pretreatment and then is undertaken the seasoning composite treatment to obtain the fishbone leisure food. The efficiency for adopting the high-temperature acidizing cooking treatment to soften the tilapia fishbone is high, and the time can be reduced by 50 to 83 percent compared with the pure high-temperature cooking treatment. The fishbone leftovers in the aquatic processing is adequately used; the bone mass of the product is uniformly crisp, so the product is easy to chew up, the flavor is good, and no bone residue exists. The calcium-phosphorus ration of the product is about 2.5:1, thereby favoring the absorption and utilization of the calcium in the human body.

In accordance with the present invention, there are provided humanized fish insulin genes. Humanized insulin the present invention encode human insulin alpha and / or beta chains while using fish-preferred codons and regulatory sequences. These humanized genes are thus expressible in fish islet cells. Also provided are transgenic fish having islet cells containing and capable of expressing humanized insulin genes. These islet cells (Brockmann Bodies) can be xenotransplanted into subjects having diabetes. In this manner normoglycemia can be achieved in the recipient of the islets.

The invention discloses a pond poly-culture method for tilapia mossambica and litopenaeus vannamei. The pond poly-culture method comprises the following steps of: adding quick lime into a dry pond in spring every year, injecting water to carry out water sterilization, adding 50 kg-100 kg of fermented chicken manure into each mu of the pond when the water temperature is stabilized to over 20 DEG C, injecting water until water depth is 40cm-60cm, waiting for 1-2 weeks, adding 8000-12000 litopenaeus vannamei shrimp seeds into each mu of the pond, and feeding special poly-culture feed; injecting water after 15-20 days until water depth is 100cm-120cm, adding 1500-2500 tilapia mossambica fries into each mu of the pond, feeding the poly-culture feed, injecting water after 15-20 days until water depth is 200cm-250cm, oxygenating for 3-7 hours every day, and mixing 20kg-30kg of the quick lime with water to spray for each mu of the pond once to twice every month; catching the tilapia mossambica and litopenaeus vannamei after 120-150 days. According to the pond poly-culture method disclosed by the invention, the special poly-culture feed is fed, prawn quality is high, water pollution is less, management is easy, cost is low and benefits are high.

The invention discloses a high-sugar and low-protein tilapia feed. Based on the total mass fraction of 100%, it consists of the following components: fish meal: 0-3%, soybean meal: 16-20%, rapeseed meal: 18-21%, cotton meal: 12-16%, secondary meal: 39- 42%, soybean oil: 2-3%, choline chloride: 0.2-0.5%, sodium chloride: 0.1-0.5%, calcium dihydrogen phosphate: 1-2%, vitamin B1: 0.004-0.006%, vitamin B6: 0.002-0.004%, zinc sulfate: 0.02-0.04%, chromium chloride: 0.0001-0.0003%, vitamin premix: 0.1-0.2% and mineral premix: 0.5-1%. The invention maximizes the amount of secondary powder in the feed, adds several plant protein sources to overcome the problem of amino acid imbalance of a single protein source, and at the same time significantly reduces the amount of fish meal. By optimizing and adjusting the feed formula, supplementing suitable vitamin and mineral premixes to adjust the sugar utilization ability of tilapia, so that the tilapia can make good use of the sugar compound of the present invention, avoiding the damage of fish liver caused by high sugar, Fatty liver is formed, and even the fish body becomes obese, and the symptoms of "big belly" appear.

The invention discloses a multi-species diversification healthy ecological breeding method. The method comprises the step of pond reconstruction. According to the pond reconstruction, a pond of 100 mu is built up into a land area, a shallow area and a deep water area, basically, one third of the pond is the land area, a half of the pond is the shallow area, and the rest is the deep water area. According to the variety throwing amount, one hundred carps, two hundred crucians, fifty spotted silver carps, twenty grass carps, five thousand pacific white shrimps, sixty turtles, three hundred river crabs and five hundred loaches are thrown. Alfalfa is planted in the land area for feeding the grass carps, lotus roots and wild rice shoots are planted in a part of the shallow area, and aquatic commercial crops such as water hyacinth and water spinach are planted in a part of the shallow area. In the deep water area, a cage is used for culturing five hundred tilapias to regulate and control the water quality. According to the planting density, three hundred and fifty kilos of the lotus roots are planted in one mu, three hundred and fifty wild rice shoots are planted in one mu, and the ratio of the medicago plant area to the culture area is larger than one to five.

The invention provides a fish meal and fish oil based on leftover bits and pieces of Tilapia mossambica and making method in preparing field of animal fodder raw material. The making method of fish meal comprises the following steps: choosing leftover bits and pieces of Tilapia mossambica as raw material; producing fish meal after boiling, pressing, drying, disintegrating, sizing and packaging. The making method of fish oil comprises the following steps: doing solid-liquid separation of pressing liquid through pressing step; centrifuging the liquid at high-speed to get upper layer oily substance being fish oil; thickening sub-layer fish dissolving pulp to return fish meal to improve yield rate. The invention processes leftover bits and pieces of Tilapia mossambica to be high grade fish meal and fish oil in changing waste into treasure way with totally-enclosed during the whole process and improves yield rate and prevents polluting environment through waste gas retracting device at the same time, which achieves one level domestic fish meal request in every index of quality belonging an environment-friendly product with fine quality that substitutes domestic fish meal absolutely.

The invention discloses a method for preparing fermented virus-free cottonseed meal feed protein. The invention relates to a production process which uses cottonseed meal as raw material and produces the virus-free cottonseed meal feed protein by microbial technology in a transformation way, in particular to a microbial fermenting process of using multi-strains mixed solid to ferment and prepare virus-free cottonseed meal. Fermentation cultures of one strain Saccharomyces cerevisiae which is cultured in an amplification way, one strain Candida tropicalis, one strain Bacillus ubtilis and one strain Lactobacillus are evenly mixed in the proportion of 2 to 1 to 1 to 1 and are inoculated in cottonseed meal (through 40 mesh sieve) with 5 percent of the inoculation amount, wherein, the water content of the cottonseed meal is 50 percent; simultaneously, substrate culture media (5 percent of molasses, 5 percent of bran, 0.5 percent of ammonium sulfate, and the initial pH value is 6.0) are added, evenly mixed, fermented for 72hours at 30 DEG C in aerobic way, dried and sampled at 60 DEG C; finally, the nutrient analysis is carried out on the cottonseed meal before and after fermentation; when weighed and packaged, finished products of the fermented cottonseed meal can be stored for 6 months at a cool and dry place. The fermented cottonseed meal can be used as feeds for beef cattle, chicken, tilapia, etc.

The invention belongs to the technical field of microcapsule preparation, which particularly relates to a powdered tilapia oil and a production method thereof. A base material is formed by tilapia oil and powder. The base material, water, a compound emulsifying agent, an antioxidant and organic acids are mixed, homogenized, sprayed and dried to form the product. The powdered forming base material is carbohydrate or protein or the mixture of carbohydrate and protein. The invention has simple process and wide source of raw materials, effectively decreases the oxidization rate of the powdered oil by adding the antioxidant, and further improves the storage stability of the powdered oil by using the organic acids. The powdered oil has the advantages of good dispersivity, good odor, high stability, and the like.

A method of using an electronic nose for detecting freshness of tilapia includes the steps: (1) respectively placing back and belly samples of tilapia to be detected into a sample container, and standing for 20-40min at the room temperature; (2) inserting a sample injection pinhead of the electronic nose into the sample container at the normal temperature, inserting an air filter filled with activated carbon into the sample container to balance air pressure, starting the electronic nose, and enabling the electronic nose to suck gas given out by the standing samples inside; (3) detecting the gas given out by the samples by the aid of a gas sensor array inside an electronic nose gas chamber, wherein detecting time is 40-60s, and cleaning time is 3-8min; (4) setting an experimental period interval as 10-12 hours; and (5) collecting data acquired by the gas sensor array, performing data processing for the acquired data, and computing so that a freshness value of the tilapia is obtained. The method is simple, convenient, rapid and effective.

The invention discloses a method for preparing fish flavor nutritional condiment. According to the method, the residual wastes fish fillet and ground meat generated in the Tilapia fillet producing process are used as the raw materials, then the raw materials are subjected to processes of pre-processing, protease composite enzyme solution, Maillard reaction, mixing, homogenizing, and spray drying, so as to obtain the fish flavor nutritional condiment. The protein in Tilapia fillet and ground meat are subjected to the protease composite enzyme solution, in order to generate small-molecular peptide and amino acid which are likely to digested and absorbed by human body; the Maillard reaction sweet process is used in order to remove fishy smell and bitter of enzyme resolution solution, so as to generate special fragrance and taste; the product is nutritional and delicious, can be directly used as a food condiment, and further can be used as the base material of other condiments.

Disclosed is a puffed feed for Tilapia and process for preparation, wherein the constituents include (by weight percent) fish flour 8-12%, soybean 20-30%, meat and bones meal 0-10%, rapeseed waste 20-30%, malt 5-10%, liquid phosphatide 0.5-1%, short flour 3-5%, maize 15-30%, calcium hydrogen phosphate 0.5-1%, composite premixed material 1-2%, soybean oil 0-0.5%, fish oil 0.5-1%.

The invention relates to a complete feed of tilapia. The technical scheme of the complete feed disclosed by the invention is as follows: the complete feed comprises the following components in percentage by weight: 0.5-4% of fish meal, 16-23.5 of soybean meal, 22-31% of rapeseed meal, 5-6% of peanut meal, 5-7% of rice bran, 5-14% of wheat bran, 14.61-20.63% of high-protein flour, 5-6% of DDGS (Distillers Dried Grains with Soluble) and 1.3-2.2% of soybean oil. The complete feed of the tilapia, disclosed by the invention, has the advantages of simple formula and easy production; in addition, through the complete feed of the tilapia, disclosed by the invention, the contents of DHA (Docose Hexaenoie Acid) and EPA (Eicosapntemacnioc Acid) in the fish flesh can be remarkably increased so that the selling price of the tilapia is increased and the economic benefit of a tilapia raiser is remarkably improved.

Certain farmed and / or breeding fish such as tilapia are filleted after the fish have been beheaded, slaughtered and had their ventral cavity opened. Cuts are made in the dorsal region and on the ventral side in the caudal region for exposing the dorsal spokes and ventral spokes, respectively. Then, separating cuts are made over the vertebral column connecting the dorsal and ventral cuts. Before peeling separation in the region of the ventral cavity, the ventral lobes are trimmed by separation of the ends of their ventral lobe portions projecting beyond the ribs. Peeling separation is completed by peeling cuts adapted to the rib contour and made with at least two degrees of freedom.

A technique for preparing the smoked tilapia slices includes such steps as washing, immersing in fumigating liquid for 5-10 min, uniformly arranging the fumigated tilapia slices on a grate of fumigating furnace, freeze-drying at 15-25 deg.C, liquid fumigating for 20-40 min, and drying at 20-30 deg.C for 30-60 min.

The invention provides Chinese herbal medicine additive for improving meat quality of java tilapia, containing by weight of: 22-30% of spatholobus stem, 30-40% of black bean, 10-15% of glycyrrhiza, 5-10% of honeysuckle, 5-10% of radix isatidis and 10-15% of hawthorn. The advantages of the invention comprise: (1) having good phagostimulant effect, (2) substantially reducing fat content and cholesterol level in muscles of java tilapia, substantially increasing flavor substance content of inosinic acid in muscles, improving muscles quality of java tilapia, (3), adding and using conveniently, and directly adding in commercial feedstuff, wherein the components can generates synergistic effect with other additive components in commercial feedstuff, and raise weighting rate and disease resistance of java tilapia, and (4) having stabile raw material resource, low price, simple production process and easy for industrialization production.

The invention discloses a kind of method for American hilsa herring ecological type cultivation, which mainly comprises controlling the main element of water environment, the oxygen sag is more than 3mg / L, the temperature is between 10-30DEG C; regularly add the probiotics compound and feed into water to improve water quality, restrain the growing and breeding of harmful bacterium; reasonably adjust the cultivation density to ensure the eating intensity of hilsa herring collectivity and abundant oxygen sag; decrease the irritated response that cause by noise,light and color; mixed cultivate tilapia and jade perch, proceed biological control for flush ulotrichineae and conch; adjust the nutrition ingood time for the special need of hilsa herring growth.

The invention discloses a feed for improving streptococcal disease resistance of tilapia, and the feed is characterized in that: the feed is tilapia base daily ration particles containing 1% and 2% (by weight) of a compound Chinese herbal medicine water extraction solution; the compound Chinese herbal medicine water extraction solution comprises: by weight, 15% of licorice, 5% of perilla leaf, 20% of cordate houttuynia, 10% of honeysuckle flower, 15% of isatis root, 15% of hawthorn, 10% of eucommia leaf and and 10% of astragalus. Before the high temperature of the summer comes, the feed containing 2% of the compound Chinese herbal medicine water extraction solution is first fed to the tilapia for strengthening cultivation, the feed containing 1% of the compound Chinese herbal medicine water extraction solution is fed in high temperature season, the streptococcal disease infection resistant capability of the tilapia can be effectively improved, the survival rate after infection of pathogen can be increased. The Chinese herbal medicine materials in the feed are easy to obtain, are low in price, and are simple in production technology. The feed added with the compound Chinese herbal medicine has better palatability, the tilapia taking the feed may have less drug residue in the body, and the feed is in accord with requirements of green healthy aquatic products.

The invention discloses a drunken tilapia snack food and a processing method thereof. Tilapia fillets are prepared into semi-dried drunken fillets through the steps of picking, inebriating, vacuum microwave drying and the like. By controlling the drying conditions, the flavoring material and original chewiness of the drunken fish are well maintained while the water content of the drunken fish is improved, and thus the drunken fish is good in flavor and taste. The process is capable of shortening the production period, reducing the production cost, and facilitating production. The drunken tilapia snack food processed by the method disclosed by the invention is natural in flesh color, compact, fresh and tender in meat, proper in salt taste, hardness and moisture, free from intermuscular fishbone, and suitable for people of all ages. The method is further suitable for small and medium freshwater fish, such as colussoma brachypomum, without or with less intermuscular fishbone.

The invention discloses a construction method of a tilapia fillet quality evaluation model based on proteins and enzymes in muscle. According to the method, indexes of quality evaluation are determined according to factors causing quality changes of tilapia; the weight of each quality evaluation index is acquired by utilizing a fuzzy analytic hierarchy process, so that a relationship between subjective evaluation standard and objective evaluation standard can be established; and a quality evaluation model is established according to the weight, and an unknown sample can be subjected to quality evaluation. The method can effectively and subjectively evaluate the quality of tilapia fillet. The system can be used by manufactures and import and export companies in the industry to complete quality evaluation of the tilapia fillet.