138 results about "Aeromonas" patented technology

The invention relates to a method for preparing an anti-stress agent used for fishing, belonging to the technical field of aquatic product compound feed. The method is characterized in that glutamine dipeptide, rhubarb extract, vitamin C, vitamin E, potassium chloride, sodium chloride, anhydrous dextrose and a carrier are taken and fully mixed to be ground and prepared into dry powder. The raw materials of the invention has the advantages of stable source, simple preparation, wide material source and low cost; due to the stress principle of fish body, the anti-stress agent not only supplements Chinese herbal medicines, but also complements western medicine of glutamine dipeptide; the combination of Chinese herbal medicines and western medicine regulates the stress of fish body, and appropriately supplements the loss of Na<>, K<> caused by stress to control the stress of fish body. When in use, the anti-stress agent can be added into feed and directly splashed; the immunity and inhibiting ability of fish body can be improved, the capacity of resisting high temperature and high density as well as infection resistance for resisting hydrophile aeromonas can be improved, and the growth of the fish body can be accelerated.

The invention relates to Bacillus amyloliquefaciens V4. The Bacillus amyloliquefaciens V4 is preserved in the China general microbiological culture collection center and has a preservation number of CGMCC NO. 10149. The Bacillus amyloliquefaciens V4 strain and/or microbial agent can produce bacteriocin for antagonizing vibrio pathogens and pathogenic aeromonas at seedling growing and culture temperatures of common culture water, inhibit vibrio vulnificus, vibrio natriegen, vibrio harveyi, black sea vibrio, aeromonas hydrophila and aeromonas salmonicida growth and breeding, effectively control aquatic animal infectious disease generation and propagating caused by vibrio pathogens and pathogenic aeromonas, reduce aquatic animal mortality and a forage coefficient and promote aquatic animal weight gain.

The invention discloses a microbial preparation, an aquatic feed and an aquaculture method and relates to the technical field of aquaculture. The microbial preparation comprises bdellovibrio and probiotic. Optimally, the probiotic is selected from any one of lactobacillus bulgaricus, lactobacillus delbrueckii, enterococcus faecalis, lactobacillus casei, lactobacillus acidophilus, rhodopseudomonaspalustris, clostridium butyricum, bacillus laterosporus, bacillus pumilus, bacillus coagulans, bacillus licheniformis, bacillus subtilis and lactobacillus plantarum. The microbial preparation has thecharacteristics of capability of increasing food intake of aquaculture animals, capability of promoting growth rate, survival rate and immunity of organisms, and the like. Besides, the microbial preparation is capable of effectively preventing and treating the problems of diseases caused by pathogenic bacteria, such as vibrio parahaemolyticus, vibrio harveyi, luminous vibrio, vibrio alginolyticus,vibrio campbellii, vibrio fluvialis, vibrio vulnificus, aeromonas, vibrio cholerae, pseudomonas and salmonella.

The invention discloses a composite microbial inoculum used for the anaerobic fermentation of kitchen wastes, belongs to the field of micro-organisms, and solves the problem of the lack of a microbial inoculum suitable for performing anaerobic fermentation by taking kitchen wastes as a main raw material at present. The composite microbial inoculum comprises, in parts by weight, 8-15 parts of acid production bacteroides DSM 15896 fermentation liquor, 25-35 parts of long-chain fatty acid syntrophic aeromonas DSM 18709 fermentation liquor, 15-25 parts of clostridium sordellii ATCC9714 fermentation liquor, 20-28 parts of combined methanosaeta JCM10134 fermentation liquor and 22-27 parts of methanospirllum hungatei DSM864 fermentation liquor, wherein the effective viable counts of the fermentation liquors of the five bacteria aforementioned achieve more than 10<9> per millilitre respectively. The composite microbial inoculum disclosed by the invention is suitable for an anaerobic fermentation process taking the kitchen wastes as the main raw material, and capable of playing the roles of stabilizing the pH value of a fermentation system, promoting to bring forward the peak of methanogenesis, and increasing the total amount of methanogenesis after being inoculated in the fermentation system.

The invention relates to a method for separating Aeromonas molluscorum producing tetrodotoxin from Takifugu fasciatus tissues and a fermentation culture method of the Aeromonas molluscorum as well as a detection method of the produced tetrodotoxin. The method for separating the Aeromonas molluscorum producing the tetrodotoxin from the Takifugu fasciatus tissues comprises a bacteria separation method, a bacteria identification method, a bacteria fermentation culture method, a competitiveness ELISA (Enzyme Linked Immunosorbent Assay) detection method and an LC-MS (Liquid Chromatograph-mass Spectrometry) detection method of the tetrodotoxin produced by the Aeromonas molluscorum, and the like. In the invention, by separating bacteria from Takifugu fasciatus ovaries and adopting a TCBS (Thiosulfate Citrate Bile (salt) Sucrose (agar)) culture medium for screening, the Aeromonas molluscorum capable of producing the tetrodotoxin is separated from blowfish bodies for the first time; the separated bacteria is identified by adopting a 16 SrDNA method; the competitiveness ELISA detection method and the LC-MS detection method are both firstly adopted for the tetrodotoxin produced by the separated Aeromonas molluscorum; and the produced tetrodotoxin and tetrodotoxin extracted from the blowfish bodies are determined to be the same substance; in addition, the separated Aeromonas molluscorum can massively separate the tetrodotoxin from the bacteria after being cultured.

The invention relates to a gene chip and an application, which belongs to the biological detection field. The invention designs a set of detection probes by aiming at 11 types of common infectious diarrheal disease pathogen microorganism in clinic, and the gene chip containing the set of the probe. The detection probes comprises a vibrio parahaemolyticus probe, a vibrio vulnificus, a vibrio cholera probe, a vibrio alginolyticus probe, a vibrio furnissii probe, a shigella probe, an escherichia coli probe, an aeromonas probe, a salmonella probe, a campylobacteria probe, and a proteusbacillus vulgaris probe; the above mentioned probe sequence is shown as SEQ ID No: 1-117. The gene chip and probe can detect 11 types of common infectious diarrheal disease pathogen, the detection flux is high, the specialty is strong, the sensitivity is high, and the detection is rapid and effective.

The invention relates to the field of fish medicine preparations, in particular to Sanhuang supper micropowder as well as a preparing method and applications thereof. The supper micropowder comprises in percentage by weight as follows: 25-35% of baical skullcap roots, 25-35% of amur cork tree bark, 25-35% of rhubarb and 5-10% of indigowoad leaves. The Sanhuang supper micropowder has effects of clearing heat, cooling blood, resisting viruses and detoxifying, plays powerful antibacterial, disinfection and antiviral roles in water bodies and pathogenic bacteria of aqueous animal (gills, body surfaces and the like) tissue and is particularly used for mainly treating bacterial enteritis caused by pseudomonas, vibrio, aeromonas hydrophila and Edwardsiella in the aqueous animals. Tests prove that the Sanhuang wall-breaking supper micropowder can improve and treat white excrement enteritis of penaeus vannamei boone and septicemia of Micropterus salmoides to a certain extent, can enhance the aeromonas hydrophlia infection resistance of the Micropterus salmoides and is a novel fish medicine for effectively protecting intestinal tracts and preventing bacterial enteritis in the aqueous animals, and the use amount can be significantly decreased by 2-3 times.

The invention relates to a pathogenic aeromonas hydrophila assay kit and belongs to the field of the detection of pathogenic microorganisms. A primer which is designed on the basis of a highly conserved interval of a standard strain J-1 strain 16S rDNA genome of the pathogenic aeromonas hydrophila is used for distinguishing and identifying strain characteristics of the pathogenic aeromonas hydrophila, and the amplified fragment is 685bp; and a second pair of primers which is designed according to specific proteinase aerolysin secreted by the strain is used for judging the pathogenicity of the aeromonas, and the fragment size is 301bp. The assay kit can rapidly and accurately judge the strain characteristics and pathogenicity of the strains to be tested. Comparison tests of the conventional biochemical identification method and pathogenicity identification method for experiment strains and clinical stains show that the coincidence rate of the kit is 100 percent. Evaluation tests for specificity, repeatability, sensitivity and retention period of the kit acquire ideal results.

An Aeromonas salmonicida subspecies (Aeromonas almonicidasubsp. masoucida) is isolated from Cynoglossus semilaevis Gunther cultured in Shandong Province, China, the preservation number of the Aeromonas almonicidasubsp. masoucida is CCTCCM2018085. A pathogenic bacteria strain of ascites disease is isolated from the Cynoglossus semilaevis Gunther for the first time. Antigens of the strain contain protective antigens against Aeromonas salmonicida, the strain is suitable for preparing various vaccines against the Aeromonas salmonicida, and is capable of reducinge losses caused by diseases in aquaculture, reducing or eliminating abuse of chemical drugs such as disinfectants and antibiotics caused by the Aeromonas salmonicida, and significantly improving the safety of aquaculture animals and products, and reducing bio-safety risks caused by increase of drug resistance of pathogenic bacteria.

The invention relates to a preparation method of a microbial bactericide, which belongs to the field of microbial fermentation industry. The invention discloses a method for preparing Vibrio anguillaris bactericide by using Pseudoalteromonas sp., which consists of (1) slant activation and culture of strains, (2) liquid fermentation culture, (3) crude fermented liquid Mention, (4) steps such as the preparation of bacteriostatic agent are formed. The method of the invention is simple, the culture medium is cheap and easy to prepare, and the liquid culture technology is adopted, which is beneficial to enlargement and industrialized production, and has stable properties and good application value.

The invention discloses a sturgeon-source aeromonas hydrophila and an application thereof. The collection number of the sturgeon-source aeromonas hydrophila is CCTCC NO:M 2016555. Inactivated vaccine prepared with the sturgeon-source aeromonas hydrophila can prevent sturgeon bacterial septicemia. The quantity of the aeromonas hydrophila HZ8 (Aeromonas HydrophilaHZ8) in the inactivated vaccine is larger than or equal to 1.0*10<8> CFU/ml. The screened sturgeon-source aeromonas hydrophila has the high pathogenicity, the inactivated vaccine prepared with the sturgeon-source aeromonas hydrophila can prevent sturgeon bacterial septicemia, and the sturgeon-source aeromonas hydrophila has good market popularization prospects.

The invention provides the DNA sequence of a lycopene cyclase gene (crtY) in sphingomonas sp., a recombinant strain lacking lycopene cyclase and use thereof. The nucleotide sequence of the crtY gene is represented by SEQ ID NO.1. The DNA sequence of the lycopene cyclase gene (crtY) in sphingomonas sp., which is provided by the invention, lays a foundation for the genetic modification of a sphingomonas sp. carotenoid biological synthesis means. In addition, compared with a wild strain, the sphingomonas sp. recombinant strain lacking the lycopene cyclase gene, which is constructed by a gene knockout method, has the advantages that: the gellengum yield is basically unchanged; and lycopene can be produced in the production of gellengum by fermentation. And the strain can be used for further gene knockout or metabolic engineering modification.

The invention provides the DNA sequences of a beta-carotene hydroxylase gene (crtZ) and a 2,2'-betahydroxylase gene (crtG) in sphingomonas sp., recombinant strains lacking beta-carotene hydroxylase and 2,2'-betahydroxylase and the use of the two strains. The nucleotide sequence of the crtZ gene is represented by SEQ ID NO.1; and the nucleotide sequence of the crtG gene is represented by SEQ ID NO.2. The DNA sequences of the beta-carotene hydroxylase gene and the 2,2'-betahydroxylase gene in the sphingomonas sp., which are provided by the invention, lay a foundation for the genetic modification of a sphingomonas sp. carotenoid biological synthesis means. In addition, compared with wild strains, the recombinant strains of the sphingomonas sp., which are constructed by a gene knockout method, have the advantages that: gellengum yield is basically unchanged; and beta-carotene or zeaxanthin can be produced in the production of gellengum by fermentation. And the strains can be used in further gene knockout or metabolic engineering modification.

The invention relates to application of Chinese herbal medicament to delaying of bacterial drug resistance. The Chinese herbal medicament comprises isatis roots, blackberry lily, radix sophorae flavescentis, folium isatidis, plantain herbs, fructus forsythiae, scutellaria baicalensis or wormwood. The Chinese herbal medicament has the advantage that the traditional Chinese medicine which is selected out has the effect of delaying aeromonas drug resistance.

The invention discloses a medicine for preventing and treating crucian carp enteritis. A medicine composition is prepared from the following ingredients in parts by weight: 20 to 30 parts of fructus chebulae, 15 to 30 parts of cortex phellodendri, 15 to 30 parts of rhizoma atractylodis, 20 to 30 parts of radix scutellariae, 5 to 10 parts of rhizoma coptidis, 7 to 8 parts of herba portulacae, 8 to 14 parts of radix aucklandiae, 10 to 15 parts of radix arnebiae seu lithospermi, 3 to 5 parts of radix rubiae, 5 to 8 parts of pomegranate bark, 20 to 30 parts of manyflower glorybower roots, 5 to 7 parts of cortex lycii, 20 to 30 parts of honeysuckle flowers, 17 to 28 parts of fructus forsythiae, 15 to 35 parts of wild chrysanthemum flowers, 18 to 32 parts of radix sanguisorbae and 8 to 15 parts of plant soot. By using the medicine in compatibility; the activity of aeromonas punctata f. intestinalis can be effectively inhibited; pathogenic bacteria and bacteria in the body can be effectively killed after the medicine is taken for many times; after the medicine composition is used, both the prevention effect and the treatment effect are achieved; the curative effect taking speed is high; the medicine is harmless to fish bodies; no medicine residue exists; no harm is caused on the human bodies after the medicine is taken; and meanwhile, the immunity of the fish bodies can be enhanced.

The invention discloses a medicine for treating white spot disease of turtle. The medicine consists of gentian violet, fructus forsythiae, blackberry lily, sodium chloride, houttuynia cordata, honeysuckle, amur cork tree bark and common cnidium fruit. When resisting fungi, the medicine disclosed by the invention has a certain capacity of inhibiting aeromonas, so that the medicine can be used for treating white spot disease of turtle. Through reasonable combination, dried orange peel, hawthorn, houttuynia cordata, honeysuckle, wild chrysanthemum, amur cork tree bark and the like can eliminate the heavy flavor of garlic when resisting the fungi. Liquorice root can be used for protecting liver so as to protect liver cells from being hurt by tannin of gallnut and the like. Through a preparation method, an active ingredient extraction ratio is improved and the loss of beneficial materials is reduced. The garlic is mixed with the dried orange peel, so that the flavor of the garlic is eliminated in advance.

The invention provides a carbon source used for regulation and control of microbial community in culture pond water body and an application, the carbon source comprises the following components by weight part: 60-100 parts of soluble starch, 5-20 parts of vegetable protein and 5-20 parts of micromolecule organic acidity sodium salt. The experiment proves that the compound carbon source is capable of reducing ammonia nitrogen concentration of the pond water body, increasing algae abundance and controlling the aeromonas quantity, accordingly, the carbon source has large application potential on regulation and control of microbial community in culture pond water body.

An antibacterial medicinef or aquatic animals, such as mandarinfish, is prepared from uritrate, VC and starch.

A light green balloon bacteriophage AVP is brought forward, deposited in China typical culture preservation center with that deposit number of CCTCC NO:M 2018155, deposited on 25 March 2018. The new light green balloon bacteriophage AVP can lyse the bacteriophage AVP lysate of Aeromonas aeruginosa, the bacteriophage has a wide lysis spectrum, not only can it lyse the pale green balloon bacteria, It can also lyse Staphylococcus aureus and Staphylococcus epidermidis. It can lyse standard strains or clinical strains isolated from environment or diseased animal feces. It can be used alone or in combination with other substances to provide a safe and non-toxic bacteriophage sterilization product for disinfection and purification of environment.

The invention discloses a medicine for treating white-spot disease caused by aeromonas. The medicine comprises fructus forsythiae, licorice root, garlic, Chinese gall, radix isatidis, rheum officinale, lonicera japonica, chrysanthemum indicum, ash bark, herba houttuyniae, Japanese buttercup herb, coptis chinensis, radix scutellariae, cortex phellodendri, pericarpium citri reticulatae, medicine terminalia fruit, sweet wormwood, rhizoma belamcandae, alisma plantago-aquatica linn, angelica sinensis, asparagus fern, fructus mume, pangolin, flos paulowniae, Japanese cayratia herb, solanum lyratum thunb and radices trichosanthis. The traditional Chinese medicine can be used for treating white-spot disease with a remarkable curative effect, and the curative rate of 30 days of the traditional Chinese medicine can be 90 percent. Garlic and other materials having spicy flavor are used, but other medicinal materials are reasonably combined, so that the aim of removing smell without removing curative effects can be achieved, and the curative effect can be further improved when the medicine is especially combined with western medicine antibiotics and added with calcined gypsum and other components.

The invention discloses an egg yolk antibody microcapsule feed additive for resisting carassius auratus gibelio bacterial septicemia. The additive consists of three kinds of raw materials, namely, egg yolk antibody freeze-dried powder for resisting the carassius auratus gibelio bacterial septicemia, astragalus powder and beta-cyclodextrin, wherein the egg yolk antibody freeze-dried powder for resisting the carassius auratus gibelio bacterial septicemia is mainly used for lowering the infection rate of carassius auratus gibelio by three kinds of mesophilic aeromonas or preventing carassius auratus gibelio from being infected by the three kinds of mesophilic aeromonas; the astragalus powder is used for enhancing the immunity of the carassius auratus gibelio; the beta-cyclodextrin is mainly used for realizing embedding and slow release of the egg yolk antibody freeze-dried powder. When the microcapsule feed additive and a commercially available carassius auratus gibelio compound feed are fed together according to a certain proportion, diseases caused by three kinds of pathogenic mesophilic aeromonas can be prevented and treated during cultivation of the carassius auratus gibelio, the yield of the carassius auratus gibelio is increased, and the economical benefit of carassius auratus gibelio cultivation is increased remarkably. The problem of bacterial septicemia in current carassius auratus gibelio production is solved.