29 results about "Aeromonas" patented technology

The invention discloses a primer probe for detecting fish pathogenic aeromonas and application of the primer probe, the primer probe is designed on the basis of high conserved region fragments of a griB gene of various aeromonas, and nucleotide sequences of the high conserved region fragments of the griB gene are shown as SEQ ID NO: 1-2. The primer probe disclosed by the invention can be used for simultaneously detecting various aeromonas through one-time reaction, the detection limit is as low as 10 copies / reaction, the detection period is short, and the primer probe has good universality and can be widely applied to the fields of aquaculture disease monitoring, food safety detection, water area microorganism ecological research and the like.

The invention belongs to the technical field of sewage treatment, and particularly relates to a preparation method of a low-temperature composite flora, a cold-resistant microbial agent for low-temperature sewage treatment and application of the cold-resistant microbial agent. The invention provides a low-temperature complex microbial community, which is prepared from flavobacterium (Flavobacterium), aeromonas (Aeromonas), sphingomonas (Sphingobacterium), pseudomonas (Pseudomonas), Comamonas (Comamonas), Massiella (Massiella) and Delftia (Delftia), and is characterized in that the low-temperature complex microbial community is prepared from the following raw materials: the flavobacterium (Flavobacterium), the aeromonas (Aeromonas), the sphingomonas (Sphingobacterium), the pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas The low-temperature composite flora can treat domestic wastewater under a low-temperature condition, and is high in nitrogen removal rate, short in treatment time, wide in application range and better in impact resistance.

The present invention belongs to the field of bioengineering, and specifically discloses a threonine aldolase mutant with improved enzyme activity and its application in the production of L-serine. The amino acid sequence of the mutant has a mutation of T140A or T140A / I118V compared to the wild-type threonine aldolase derived from Aeromonas. Experiments show that, after analysis of enzyme activity assays of induced expression and purification, the enzyme activity of the mutant is 1.6 and 2.9 times higher than that of the wild type, respectively, and the thermal stability is significantly improved. The beneficial mutants provided by the present invention can lay a good foundation for the industrial production of L-serine and downstream derivatives such as L-cysteine.

This invention discloses a monoclonal antibody against Aeromonas hydrophila and its application. The monoclonal antibody against Aeromonas hydrophila includes monoclonal antibody 275-4 or its antigen-binding portion, and monoclonal antibody 275-5 or its antigen-binding portion. The monoclonal antibody or its antigen-binding portion prepared in this application exhibits high specificity and sensitivity in detecting Aeromonas hydrophila. A colloidal gold test strip prepared using monoclonal antibody 275-4 or its antigen-binding portion and monoclonal antibody 275-5 or its antigen-binding portion can detect Aeromonas hydrophila in biological samples or aquaculture water. This test strip has high sensitivity and specificity, thus exhibiting good detection accuracy. Furthermore, this detection method does not require expensive instruments, is simple and convenient to operate, and can obtain results rapidly in a short time (10-15 minutes), which is beneficial for early disease diagnosis and has good clinical application prospects.

This invention belongs to the field of molecular biology, specifically disclosing a primer set, kit, method, and application for detecting Aeromonas dacca. The primer set includes an upstream primer DK1F and a downstream primer DK1R. The nucleotide sequence of the upstream primer DK1F is shown in SEQ ID NO.1, and the nucleotide sequence of the downstream primer DK1R is shown in SEQ ID NO.2. This invention provides a novel genetic marker, DK1, as a new target for Aeromonas dacca, and designs a corresponding primer set. The detection method based on this primer set exhibits high specificity and sensitivity, achieving a sensitivity of 1.38 cfu / μL for Aeromonas dacca bacterial suspension detection and 9.37 × 10⁻⁶ for nucleic acid detection. ‑4 With a concentration of ng / μL, it can rapidly and accurately detect Aeromonas dacarba in a variety of biological and environmental samples.

The application discloses an Aeromonas phage recombinant system, an expression vector, a recombinant strain and application thereof, and the recombinant system comprises a recombinase operon 109TES derived from a salmonella Aeromonas phage vB_AsaM-56; the operon comprises at least one gene with exonuclease function and one single-stranded DNA annealing protein gene. Through function verification in Escherichia coli, 109TES can efficiently mediate linear circle recombination and linear linear recombination by using a short homologous arm (50 bp), and has stable and reliable recombination function in a heterologous host, indicating that the recombinase operon from a non-Escherichia coli phage can also exert efficient homologous recombination function in Escherichia coli, and can be used as a powerful candidate for constructing a new type of recombination tool, and provides a new choice for developing a multi-host available gene editing system.

The present application relates to a kind of pseudomonas aeruginosa engineering bacteria DUEC construction method and application.The DUEC engineering strain construction method of the present application is: response to kinase coding gene retS and sheath protein coding gene tssB2, tssB3 Knockout, H1-T6SS secretory effector protein Tse1-Tse4, Tse6-Tse8 catalytic site mutation, Tse5 Coding gene knockout.DUEC engineering strain can not only constitutively express and assemble H1-T6SS, but also can respond to adjacent sister cell source T6SS attack, assemble H1-T6SS at the position of being attacked.The present application constructs a series of effector protein combination inactivation mutant by gene knockout or catalytic site mutation method.Microscope observation and statistical analysis results show that no single effector protein is necessary for T6SS attack and assembly.By bacterial competition experiment, it is verified that all effector protein inactivation mutant 8eff c Lose T6SS + Vibrio cholerae and T6SS + Aeromonas dhaka's bactericidal activity, but 8eff c Can respond to T6SS physical attack, Tse6 N -Cre is specifically delivered to T6SS + In Vibrio cholerae.

This invention relates to the field of microbial technology, and more particularly to a broad-spectrum Aeromonas hydrophila phage and its applications. The Aeromonas hydrophila phage AhP2505006 is classified as follows: Aeromonas hydrophila Phage AhP2505006, with accession number CCTCC NO: M20251307, exhibits cross-species lytic activity. It can effectively lyse *Aeromonas*, *Vibrio parahaemolyticus* and *Vibrio alginolyticus*, and *Citrobacter freundii*. It also possesses excellent targeting ability against multidrug-resistant strains and broad host spectrum characteristics, achieving a lysis rate of up to 80% against multidrug-resistant *Aeromonas hydrophila*. In the prevention and control of diseases caused by various pathogens, or in practical scenarios facing the limitations of traditional antibiotics and highly specific bacteriophages, it has become a highly promising solution.

A method of culturing an aquaculture of an aquatic animal species of interest is disclosed. The method comprises inoculating the aquaculture with bacteria of at least one genera selected from the group consisting of Pseudomonas, Limnohabitans, Janthinobacterium, Stenotrophomonas, Aeromonas, Acinetobacter and Morganella, wherein the bacteria are provided in an amount sufficient to increase the survival and / or weight of the aquatic animal, thereby culturing the aquaculture of the aquatic animal species.

The invention discloses a microbial composite flora for treating aquaculture tail water and application of the microbial composite flora, the microbial composite flora comprises aeromonas bivalve, shewanella, bacillus paralicheniformis and acinetobacter, and the ratio of the viable count of the aeromonas bivalve to the viable count of the shewanella to the viable count of the bacillus paralicheniformis to the viable count of the acinetobacter is (1-3): (2-3.5): (0.5-1.5): (1.5-2.5). Through synergistic interaction among the strains, tricaine mesylate pollutants, total phosphorus, total nitrogen and COD (Chemical Oxygen Demand) in the aquaculture tail water can be effectively degraded, and the strain has excellent properties of low temperature resistance and high salt resistance.

The invention relates to a synbiotic enzyme as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture. The synbiotic enzyme is prepared from garlic, brown sugar, radix astragali, fructus schisandrae chinensis, fructus chebulae, lactobacillus hirsuti liquid and water. The synbiotic enzyme disclosed by the invention has a broad-spectrum antibacterial effect, and has a relatively strong inhibition effect on bacterial pathogenic bacteria such as vibrio alginolyticus, listonella anguillarum, edwardsiella ictaluri, aeromonas hydrophila, vibrio parahaemolyticus, aeromonas veronii and vibrio cholerae; the composition can be used for preventing and treating hemorrhagic ascites disease, necrotic canker and other aquaculture diseases caused by bacteria and pathogenic bacteria in aquaculture, can improve the feed conversion rate in aquaculture, and improves the growth and reproduction capability, immunity and culture benefits of aquatic animals.

The present invention relates to a brown rice fermentation product and its uses, more specifically, to the use of the brown rice fermentation product to moisten the intestines, improve bowel movements, and contribute to the regulation of the intestinal microbiota. The present invention has found that the brown rice fermentation product can improve constipation, have NO removal effects, and down-regulate the relative expression levels of the nos1, nos2a, and nos2b genes. Treatment with the brown rice fermentation product can regulate the species richness of the intestinal microbiota of constipated zebrafish, with certain effects on species uniformity and diversity, increasing the abundance of beneficial bacteria (Bacteroidetes) and decreasing the abundance of harmful bacteria (Firmicutes, Aeromonas, Enterobacteriaceae). The brown rice fermentation product of the present invention can be widely used in the food industry as a functional ingredient.

This invention relates to the field of microbial technology, specifically to a thermoresistant Aeromonas guinea pig bacteriophage, its composition, and its applications. The bacteriophage was deposited on June 14, 2024, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 45946, and named RKP-AE24003. The composition comprises the aforementioned bacteriophage. The bacteriophage can be used to prepare products that kill or inhibit Aeromonas guinea pig; and products for the prevention and / or treatment of aquatic diseases caused by Aeromonas guinea pig. This bacteriophage RKP-AE24003 exhibits good thermoresistance and strong genetic stability, allowing for stable storage in high-temperature environments, significantly improving the stability of industrial production and its applicability to high-temperature scenarios.

The present application relates to the field of natural pharmaceutical chemistry and microbial medicine technology, and particularly relates to a novel polyketide compound extracted from a fermentation product of a fungus Fusarium asiaticum, a method for separating and purifying the polyketide compound, and application of the polyketide compound in anti-Gram-negative bacteria activity. 22 H 30 O3 (compound 1) and C 24 H 34 O2 (compound 2), as shown in 1 and 2 in formula I. The compound 1 and the compound 2 exhibit strong anti-Gram-negative bacteria activity. Among them, the compound 1 can significantly inhibit the growth of Gram-negative bacteria Aeromonas hydrophilia, Vibrio alginolyticus and V. harveyi, and the minimum inhibitory concentration (MIC) is 1.0, 2.0 and 2.0 μg / mL respectively; the compound 2 can significantly inhibit the growth of Gram-negative bacteria V. harveyi, and the MIC is 2.0 μg / mL. The compound 1 and the compound 2 have the potential to become new Gram-negative bacteria prevention and treatment drugs, and are expected to provide a new direction for solving the problems in the field.

The invention discloses recombinant aeromonas as well as a construction method and application thereof, and relates to the technical field of synthetic biology. According to the recombinant aeromonas, one or more of ExoA, GCAT, MpA, AspA, AhpB and MsbB genes are down-regulated or knocked out. According to the invention, a plurality of toxic factors (ExoA, GCAT, MpA, AspA, AhpB and MsbB) aiming at eukaryotic hosts are knocked out from the aeromonas, so that the improved recombinant aeromonas is constructed. Test results show that the recombinant aeromonas disclosed by the invention can efficiently express recombinant protein and has excellent biological safety in eukaryotic cells and animal models.

The invention relates to an exhaled air condensate microorganism combination for predicting benign and malignant pulmonary nodules, a kit and application, and the exhaled air condensate microorganism combination for predicting benign and malignant pulmonary nodules comprises pneumococcus bartgooensis, twin coccus, streptococcus, Rosella, methylobacterium and marine colibacillus. Aeromonas kirschenii and yellow aureomonas sp. The invention discloses a kit for predicting benign and malignant pulmonary nodules. The kit comprises internal standard substances which are used for detecting the contents of pneumococcus bartgoeni, twin coccus, streptococcus, Rosella, methylobacterium, marine colibacillus, aeromonas kirschenii and yellow aureomonas in an exhaled gas condensate sample extraction solution. The combined marker provided by the invention has the characteristics of high sensitivity and high specificity in diagnosis of benign and malignant pulmonary nodules.

The invention discloses an aeromonas bacteriophage recombination system, an expression vector, a recombination strain and application of the aeromonas bacteriophage recombination system. The recombination system comprises a recombinase operon 109TES derived from aeromonas salmonicida bacteriophage vBAsaM-56; the operon comprises at least one gene with exonuclease function and a single-stranded DNA annealing protein gene. Through functional verification in Escherichia coli, 109TES can utilize a short homologous arm (50bp) to efficiently mediate wire loop recombination and wire-line recombination, and has a stable and dependable recombination function in a heterologous host, which indicates that a recombinase operon from a non-Escherichia coli bacteriophage can also exert an efficient homologous recombination function in the Escherichia coli, so that the recombinant enzyme operon can be used for preparing a recombinant vector. The method can be used as a powerful candidate for constructing a novel recombination tool, and a new choice is provided for developing a multi-host available gene editing system.

The application belongs to the technical field of microorganisms, and provides a daka aeromonas bacteriophage vB_AdhP_L12 and an application thereof. The taxonomic name of a daka aeromonas bacteriophage vB_AdhP_L12 is daka aeromonas bacteriophage vB_AdhP_L12 Aeromonas dhakensis The daka aeromonas bacteriophage vB_AdhP_L12 has a preservation number of CCTCC NO: M 2026249, and was preserved in the China Center for Type Culture Collection (CCTCC) on January 26, 2026. The application enriches the treatment strategy for daka aeromonas, and lays a foundation for inhibiting daka aeromonas disease infection in crocodile breeding.

This invention provides a nanopore detection device, as well as a method and application for detecting biological samples using this device. The nanopore detection device includes an insulator with openings, a phospholipid membrane or polymer membrane located within the openings, nanopores formed by mutant aeromonas lysin passing through the phospholipid membrane or polymer membrane, chambers located on opposite sides of the insulator, an electrolyte contained within the chambers, and a detector disposed within the chambers. The nanopore technology developed in this application enables efficient qualitative or rapid quantitative detection of one or more samples—such as one or more angiotensin peptides—and achieves significant improvements in resolution, sensitivity, capture efficiency, and matrix compatibility. The nanopore technology developed in this application also enables real-time quantitative monitoring of the dynamic changes of one or more samples—such as one or more angiotensin peptides.

The invention relates to an aeromonas rapid detection kit in the technical field of aeromonas, the aeromonas rapid detection kit comprises a top seat and a base, an inner shell is fixed between the top seat and the base, the outer wall of the top seat is rotatably connected with an annular cover through a bearing, and an outer shell attached to the outer wall of the inner shell is fixed at the bottom of the annular cover; the multi-station assembly is arranged in the inner shell, and a driving mechanism used for driving the multi-station assembly to rotate in a stepping mode is arranged on the top base. And the pressure applying assembly is partially arranged on the top seat, and partially extends into the inner shell. Multiple samples can be processed at a time, compared with a single-channel detection mode, the detection efficiency is fully improved, meanwhile, the to-be-detected sample is rapidly diffused and permeates aeromonas test paper under the action of airflow blowing and pressure intensity, the problem that the sample is slowly diffused in traditional test paper detection is solved, and the requirement for rapid detection is met.

The present invention discloses a strain of Bacillus velezensis D34 with both biocontrol and water regulation functions and its applications. The D34 strain has a deposit number of CCTCC NO: M 2019457, and is deposited with the China Center for Type Culture Collection at Wuhan University in Wuhan, Hubei Province, China, on June 17, 2019. The strain has an inhibitory effect on the fish pathogen Gill mold, protects fish against infection by the pathogen Aeromonas, and effectively degrades ammonia nitrogen and nitrite, toxic substances in aquaculture water.

The application relates to an Aeromonas rapid detection kit in the technical field of Aeromonas, which comprises a top base and a bottom base, an inner shell is fixed between the top base and the bottom base, an annular cover is rotationally connected to the outer wall of the top base through a bearing, the bottom of the annular cover is fixed with an outer shell which is attached to the outer wall of the inner shell, a multi-station assembly is arranged in the inner shell, a driving mechanism for driving the multi-station assembly to step-rotate is arranged on the top base, and a pressure applying assembly is partially arranged on the top base and partially extends into the inner shell. The application can process multiple samples at a time, the detection efficiency is greatly improved compared with a single-channel detection mode, the to-be-detected sample is rapidly diffused and penetrates the Aeromonas test paper under the action of airflow blowing and pressure, the problem of slow sample diffusion in traditional test paper detection is solved, and the requirement of rapid detection is met.

The application discloses application of a composition containing cold land berries and cold land medicinal and edible Chinese herbs in improving intestinal flora function of Alzheimer's disease. The cold land berries contain blueberries, wild cranberries and blue honeysuckle fruits; the cold land medicinal and edible Chinese herbs include acanthopanax powder, schisandra extract and astragalus extract. Preferably, the composition further contains grape seed extract and vitamin C powder. Experiments prove that the composition containing the cold land berries and the cold land medicinal and edible Chinese herbs can improve richness and uniformity of intestinal flora, and can significantly down-regulate six genera of Staphylococcus (Staphylococcus), Vibrio (Vibrio), Collinsella (Collinsella), Actinomycetales (Actinomycetales), Aeromonas (Aeromonas) and Holdemanella (Holdemanella) which are closely related to Alzheimer's disease, so as to achieve the purpose of improving intestinal flora function of Alzheimer's disease.

The invention belongs to the technical field of wastewater treatment, and particularly relates to a bacterial agent for treating benzalkonium chloride-containing wastewater and an application thereof. The bacterial agent is obtained by regulating and controlling experimental environmental parameters and domesticating the bacterial agent, wherein the mass concentration percentage of each bacterial species is 32.37% of Pseudomonas, 21.94% of Rhizobium, 14.69% of Achromobacter, 4.65% of Stenotrophomonas, 3.79% of T. mobilis, 2.41% of Flavobacterium, 2.26% of Alcaligenes, 1.88% of Acetogenic Proteinophilus, 1.59% of Bacillus and 0.53% of Aeromonas. The bacterial agent can improve the degradation rate of COD in the benzalkonium chloride-containing wastewater.

The invention belongs to the technical field of monad detection, and particularly relates to a kit for detecting aeromonas in aquatic products, which comprises an operation plate for uniformly and quantitatively adding samples to a plurality of PCR tubes in a PCR plate; the operation plate comprises a plurality of quantitative latex containers made of elastic materials, injection ports of the quantitative latex containers are fixed relative to the upper plate, the upper plate does not seal the injection ports of the quantitative latex containers, the sealed ends of the quantitative latex containers are fixed relative to the lower frame, and the upper plate and the lower frame move relatively linearly to be away from or close to each other. The volume of the quantitative latex container can be changed after the quantitative latex container is stretched, the distance between the upper plate and the lower frame corresponds to the fixed volume of the quantitative latex container, and when the quantitative latex container is used, the volume of the quantitative latex container is adjusted according to the volume of a solution needing to be filled into each PCR tube, so that the maximum volume of the quantitative latex container at the moment is the volume of a target solution filled into the PCR tube.

The invention belongs to the technical field of microorganisms, and provides an aeromonas dakara phage vBAdhPL12 and application thereof, the taxonomic name of the aeromonas dakara phage vBAdhPL12 is aeromonas dakara phage vBAdhPL12, the preservation number of the aeromonas dakara phage vBAdhPL12 is CCTCC NO: M 2026249, and the aeromonas dakara phage vBAdhPL12 is preserved in the China Center for Type Culture Collection (CCTCC) on January 26, 2026. According to the invention, the treatment strategy of the Aeromonas dakara is enriched, and a foundation is laid for inhibiting the infection of the Aeromonas dakara disease in crocodile culture.

This invention provides applications of the pfaR and pfaK genes of *Aeromonas dacca*, belonging to the field of microbial technology. This invention is the first to identify a previously uncharacterized OmpR / PhoB family response regulator, pfaR, and hypothesizes that pfaR and pfaK constitute a functionally paired two-component system. Experiments show that pfaR can promote the virulence of *Aeromonas dacca* and directly regulate argF1 to promote its biofilm formation. This invention provides new molecular evidence for understanding the pathogenic regulatory network of *Aeromonas dacca* and lays a theoretical foundation for the development of antiviral drug targets.

The invention relates to the technical field of microorganisms and application thereof, in particular to aeromonas eel, a microbial agent containing the aeromonas eel and application of the aeromonas eel to promotion of nitrogen fertilizer absorption of plants. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34412. The aeromonas eel provided by the invention is separated and screened by the inventor for the first time, and has the capability of promoting plants to absorb nitrogen fertilizer. In addition, the strain also has the functions of dissolving phosphorus, potassium and silicon, is a multifunctional strain, and can promote plant growth and activate soil nutrients.

The present invention belongs to the field of environmental protection and green and healthy breeding, and specifically relates to a broad-spectrum phage and its probiotic preparation and application. The broad-spectrum phage is phage YTLJ-VBN1, which was deposited in the Guangdong Provincial Microbial Culture Collection Center on January 22, 2024, with a deposit number of GDMCC No: 64300-B1, classified as Vibrio phage, and the deposit address is Guangzhou, China. Broad-spectrum phage can simultaneously and efficiently kill a variety of highly pathogenic Vibrio, Aeromonas, Edwardsiella and other pathogens, which can overcome the problem of the narrow bactericidal spectrum of traditional phages and can solve the problem of drug resistance that is easy to develop during the process of antibiotic prevention and treatment of pathogens in an ecologically friendly way.