138 results about "Aeromonas" patented technology

Butane-utilizing bacteria are used to degrade hydrocarbon pollutants such as trichloroethene (TCE). In-situ or ex-situ techniques may be used to reduce or eliminate hydrocarbon pollutants from liquid, gas and solid sources. In a preferred embodiment, TCE concentrations in various aqueous environments are reduced by contacting a contaminated water source with butane-utilizing bacteria in the presence of oxygen to degrade the TCE by cometabolism or direct metabolism. Suitable butane-utilizing bacteria include Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium and Cytophaga. The butane-utilizing bacteria have relatively low TCE toxicity in comparison with conventional methane-utilizing bacteria, and demonstrate an improved ability to degrade TCE.

The invention relates to a method for preparing an anti-stress agent used for fishing, belonging to the technical field of aquatic product compound feed. The method is characterized in that glutamine dipeptide, rhubarb extract, vitamin C, vitamin E, potassium chloride, sodium chloride, anhydrous dextrose and a carrier are taken and fully mixed to be ground and prepared into dry powder. The raw materials of the invention has the advantages of stable source, simple preparation, wide material source and low cost; due to the stress principle of fish body, the anti-stress agent not only supplements Chinese herbal medicines, but also complements western medicine of glutamine dipeptide; the combination of Chinese herbal medicines and western medicine regulates the stress of fish body, and appropriately supplements the loss of Na<>, K<> caused by stress to control the stress of fish body. When in use, the anti-stress agent can be added into feed and directly splashed; the immunity and inhibiting ability of fish body can be improved, the capacity of resisting high temperature and high density as well as infection resistance for resisting hydrophile aeromonas can be improved, and the growth of the fish body can be accelerated.

A test medium and method for detecting, quantifying, identifying and differentiating up to four (4) separate biological materials in a test sample. A test medium is disclosed which allows quantifying and differentiating under ambient light aggregates of biological entities producing specific enzymes, which might include general coliforms, E. coli, Aeromonas, and Salmonella in a single test medium. A new class of nonchromogenic substrates is disclosed which produce a substantially black, non-diffusible precipitate. This precipitate is not subject to interference from other chromogenic substrates present in the test medium. In one embodiment, the substrates are selected such that E. coli colonies present in the test medium show as substantially black, general coliforms colonies show in the test medium as a blue-violet color, Aeromonas colonies present in the test medium show as a generally red-pink color, and Salmonella colonies show as a generally teal-green color. Other microorganisms and color possibilities for detection and quantification thereof are also disclosed. An inhibitor and method for making a test medium incorporating the inhibitor are disclosed.

Butane-utilizing bacteria are used to degrade hydrocarbon pollutants such as trichloroethene (TCE). In-situ or ex-situ techniques may be used to reduce or eliminate hydrocarbon pollutants from liquid, gas and solid sources. In a preferred embodiment, TCE concentrations in various aqueous environments are reduced by contacting a contaminated water source with butane-utilizing bacteria in the presence of oxygen to degrade the TCE by cometabolism or direct metabolism. Suitable butane-utilizing bacteria include Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium and Cytophaga. The butane-utilizing bacteria have relatively low TCE toxicity in comparison with conventional methane-utilizing bacteria, and demonstrate an improved ability to degrade TCE.

The invention relates to Bacillus amyloliquefaciens V4. The Bacillus amyloliquefaciens V4 is preserved in the China general microbiological culture collection center and has a preservation number of CGMCC NO. 10149. The Bacillus amyloliquefaciens V4 strain and / or microbial agent can produce bacteriocin for antagonizing vibrio pathogens and pathogenic aeromonas at seedling growing and culture temperatures of common culture water, inhibit vibrio vulnificus, vibrio natriegen, vibrio harveyi, black sea vibrio, aeromonas hydrophila and aeromonas salmonicida growth and breeding, effectively control aquatic animal infectious disease generation and propagating caused by vibrio pathogens and pathogenic aeromonas, reduce aquatic animal mortality and a forage coefficient and promote aquatic animal weight gain.

The invention discloses a microbial preparation, an aquatic feed and an aquaculture method and relates to the technical field of aquaculture. The microbial preparation comprises bdellovibrio and probiotic. Optimally, the probiotic is selected from any one of lactobacillus bulgaricus, lactobacillus delbrueckii, enterococcus faecalis, lactobacillus casei, lactobacillus acidophilus, rhodopseudomonaspalustris, clostridium butyricum, bacillus laterosporus, bacillus pumilus, bacillus coagulans, bacillus licheniformis, bacillus subtilis and lactobacillus plantarum. The microbial preparation has thecharacteristics of capability of increasing food intake of aquaculture animals, capability of promoting growth rate, survival rate and immunity of organisms, and the like. Besides, the microbial preparation is capable of effectively preventing and treating the problems of diseases caused by pathogenic bacteria, such as vibrio parahaemolyticus, vibrio harveyi, luminous vibrio, vibrio alginolyticus,vibrio campbellii, vibrio fluvialis, vibrio vulnificus, aeromonas, vibrio cholerae, pseudomonas and salmonella.

The invention discloses a composite microbial inoculum used for the anaerobic fermentation of kitchen wastes, belongs to the field of micro-organisms, and solves the problem of the lack of a microbial inoculum suitable for performing anaerobic fermentation by taking kitchen wastes as a main raw material at present. The composite microbial inoculum comprises, in parts by weight, 8-15 parts of acid production bacteroides DSM 15896 fermentation liquor, 25-35 parts of long-chain fatty acid syntrophic aeromonas DSM 18709 fermentation liquor, 15-25 parts of clostridium sordellii ATCC9714 fermentation liquor, 20-28 parts of combined methanosaeta JCM10134 fermentation liquor and 22-27 parts of methanospirllum hungatei DSM864 fermentation liquor, wherein the effective viable counts of the fermentation liquors of the five bacteria aforementioned achieve more than 10<9> per millilitre respectively. The composite microbial inoculum disclosed by the invention is suitable for an anaerobic fermentation process taking the kitchen wastes as the main raw material, and capable of playing the roles of stabilizing the pH value of a fermentation system, promoting to bring forward the peak of methanogenesis, and increasing the total amount of methanogenesis after being inoculated in the fermentation system.

The invention relates to a method for separating Aeromonas molluscorum producing tetrodotoxin from Takifugu fasciatus tissues and a fermentation culture method of the Aeromonas molluscorum as well as a detection method of the produced tetrodotoxin. The method for separating the Aeromonas molluscorum producing the tetrodotoxin from the Takifugu fasciatus tissues comprises a bacteria separation method, a bacteria identification method, a bacteria fermentation culture method, a competitiveness ELISA (Enzyme Linked Immunosorbent Assay) detection method and an LC-MS (Liquid Chromatograph-mass Spectrometry) detection method of the tetrodotoxin produced by the Aeromonas molluscorum, and the like. In the invention, by separating bacteria from Takifugu fasciatus ovaries and adopting a TCBS (Thiosulfate Citrate Bile (salt) Sucrose (agar)) culture medium for screening, the Aeromonas molluscorum capable of producing the tetrodotoxin is separated from blowfish bodies for the first time; the separated bacteria is identified by adopting a 16 SrDNA method; the competitiveness ELISA detection method and the LC-MS detection method are both firstly adopted for the tetrodotoxin produced by the separated Aeromonas molluscorum; and the produced tetrodotoxin and tetrodotoxin extracted from the blowfish bodies are determined to be the same substance; in addition, the separated Aeromonas molluscorum can massively separate the tetrodotoxin from the bacteria after being cultured.

The invention relates to a gene chip and an application, which belongs to the biological detection field. The invention designs a set of detection probes by aiming at 11 types of common infectious diarrheal disease pathogen microorganism in clinic, and the gene chip containing the set of the probe. The detection probes comprises a vibrio parahaemolyticus probe, a vibrio vulnificus, a vibrio cholera probe, a vibrio alginolyticus probe, a vibrio furnissii probe, a shigella probe, an escherichia coli probe, an aeromonas probe, a salmonella probe, a campylobacteria probe, and a proteusbacillus vulgaris probe; the above mentioned probe sequence is shown as SEQ ID No: 1-117. The gene chip and probe can detect 11 types of common infectious diarrheal disease pathogen, the detection flux is high, the specialty is strong, the sensitivity is high, and the detection is rapid and effective.

The invention relates to the field of fish medicine preparations, in particular to Sanhuang supper micropowder as well as a preparing method and applications thereof. The supper micropowder comprises in percentage by weight as follows: 25-35% of baical skullcap roots, 25-35% of amur cork tree bark, 25-35% of rhubarb and 5-10% of indigowoad leaves. The Sanhuang supper micropowder has effects of clearing heat, cooling blood, resisting viruses and detoxifying, plays powerful antibacterial, disinfection and antiviral roles in water bodies and pathogenic bacteria of aqueous animal (gills, body surfaces and the like) tissue and is particularly used for mainly treating bacterial enteritis caused by pseudomonas, vibrio, aeromonas hydrophila and Edwardsiella in the aqueous animals. Tests prove that the Sanhuang wall-breaking supper micropowder can improve and treat white excrement enteritis of penaeus vannamei boone and septicemia of Micropterus salmoides to a certain extent, can enhance the aeromonas hydrophlia infection resistance of the Micropterus salmoides and is a novel fish medicine for effectively protecting intestinal tracts and preventing bacterial enteritis in the aqueous animals, and the use amount can be significantly decreased by 2-3 times.

The present invention relates to microbiology technology, and is especially microbial preparation for repairing aniline contaminated water and its preparation process and usage. The microbial preparation consists of aerobes Pseudomonas, Alealigenes, Zoogloea, Aeromonas, Arthrobacter and Bacillus in the weight ratio of 8-10 to 3-4 to 1-2 to 1-3 to 1-2 to 1-2, as well as culture liquid containing aniline. It is applied into aniline contaminated water to reach aniline degrading rate up to 80-100 %. It is prepared through domestication in relatively high concentration nitro arene compound and nutritious compound and screening. It has high growth and reproduction capacity, powerful adaptability, high biomass, low cost, powerful water purifying effect and no secondary pollution.

The invention relates to a pathogenic aeromonas hydrophila assay kit and belongs to the field of the detection of pathogenic microorganisms. A primer which is designed on the basis of a highly conserved interval of a standard strain J-1 strain 16S rDNA genome of the pathogenic aeromonas hydrophila is used for distinguishing and identifying strain characteristics of the pathogenic aeromonas hydrophila, and the amplified fragment is 685bp; and a second pair of primers which is designed according to specific proteinase aerolysin secreted by the strain is used for judging the pathogenicity of the aeromonas, and the fragment size is 301bp. The assay kit can rapidly and accurately judge the strain characteristics and pathogenicity of the strains to be tested. Comparison tests of the conventional biochemical identification method and pathogenicity identification method for experiment strains and clinical stains show that the coincidence rate of the kit is 100 percent. Evaluation tests for specificity, repeatability, sensitivity and retention period of the kit acquire ideal results.

The invention provides a multiplex PCR method capable of simultaneously detecting 7 virulence genes of Aeromonas. The invention comprises the following steps: screening of primer combination and primer sequences; a preparation method of pathogenic bacteria DNA template; multiplex PCR reaction system and PCR amplification; and multiplex PCR product detection and result interpretation. The multiplexPCR detection method can efficiently, specifically and sensitively detect and identify the presence of Aeromonas in a sample and 1-7 virulence genes through two PCR reactions. The method is applicable to the rapid identifying of pathogenic strains and common strains of Aeromonas fish, and is applicable to molecular-assisted diagnosis of fish Aemmonasis and confirmed diagnosis of such disease. Themethod is applicable to the whole process monitoring of aquaculture animal breeding, is applicable to different sample types including water bodies, sediments, farm animals and aquatic products, monitors whether the sample contains Aeromonas or not and that the strain is common or pathogenic Aeromonas, and can identify the virulence gene types and relative expression levels of pathogenic Aeromonas.

An Aeromonas salmonicida subspecies (Aeromonas almonicidasubsp. masoucida) is isolated from Cynoglossus semilaevis Gunther cultured in Shandong Province, China, the preservation number of the Aeromonas almonicidasubsp. masoucida is CCTCCM2018085. A pathogenic bacteria strain of ascites disease is isolated from the Cynoglossus semilaevis Gunther for the first time. Antigens of the strain contain protective antigens against Aeromonas salmonicida, the strain is suitable for preparing various vaccines against the Aeromonas salmonicida, and is capable of reducinge losses caused by diseases in aquaculture, reducing or eliminating abuse of chemical drugs such as disinfectants and antibiotics caused by the Aeromonas salmonicida, and significantly improving the safety of aquaculture animals and products, and reducing bio-safety risks caused by increase of drug resistance of pathogenic bacteria.

The invention relates to growth-inhibitory composition against pathogenic bacteria of meat based food stuff comprising IgY. The IgY is a specific immunoglobulin derived from yolk of egg. 12 representative bacteria and other microbes deteriorating quality of process meat products that the IgY of the invention targets include Aeromonas hydrophila, Bacillus cereus, which comprises Aeromonas hydrophil, Bacillus cereus, Camphlobacter jejuni, Clotridium perfringens, 0157:H7 (Escherichia col, 0157:H7), Lactobacillus, Listeria monocytogens, Saccharomyces cerevisiae, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus aureus, Staphylococcus epidermidis. The 12 representative bacteria antigen forms are respectively injected the interior of chicks to obtain the IgY of the 12 antigens.

The invention relates to a preparation method of a microbial bactericide, which belongs to the field of microbial fermentation industry. The invention discloses a method for preparing Vibrio anguillaris bactericide by using Pseudoalteromonas sp., which consists of (1) slant activation and culture of strains, (2) liquid fermentation culture, (3) crude fermented liquid Mention, (4) steps such as the preparation of bacteriostatic agent are formed. The method of the invention is simple, the culture medium is cheap and easy to prepare, and the liquid culture technology is adopted, which is beneficial to enlargement and industrialized production, and has stable properties and good application value.

The invention discloses a sturgeon-source aeromonas hydrophila and an application thereof. The collection number of the sturgeon-source aeromonas hydrophila is CCTCC NO:M 2016555. Inactivated vaccine prepared with the sturgeon-source aeromonas hydrophila can prevent sturgeon bacterial septicemia. The quantity of the aeromonas hydrophila HZ8 (Aeromonas HydrophilaHZ8) in the inactivated vaccine is larger than or equal to 1.0*10<8> CFU / ml. The screened sturgeon-source aeromonas hydrophila has the high pathogenicity, the inactivated vaccine prepared with the sturgeon-source aeromonas hydrophila can prevent sturgeon bacterial septicemia, and the sturgeon-source aeromonas hydrophila has good market popularization prospects.

The invention provides the DNA sequence of a lycopene cyclase gene (crtY) in sphingomonas sp., a recombinant strain lacking lycopene cyclase and use thereof. The nucleotide sequence of the crtY gene is represented by SEQ ID NO.1. The DNA sequence of the lycopene cyclase gene (crtY) in sphingomonas sp., which is provided by the invention, lays a foundation for the genetic modification of a sphingomonas sp. carotenoid biological synthesis means. In addition, compared with a wild strain, the sphingomonas sp. recombinant strain lacking the lycopene cyclase gene, which is constructed by a gene knockout method, has the advantages that: the gellengum yield is basically unchanged; and lycopene can be produced in the production of gellengum by fermentation. And the strain can be used for further gene knockout or metabolic engineering modification.

The present invention belongs to the fields of development and application of microorganisms, and particularly discloses bacillus velezensis YFI-4 and an application thereof in preparation of an aquatic bacterium bacteriostatic agent. A preservation number of the bacillus velezensis YFI-4 is CCTCC NO:M2019653. The provided bacillus velezensis has good inhibition effects on aquatic pathogenic bacteria of aeromonas veronii, aeromonas hydrophila, pseudomonas fluorescens, yersinia ruckeri, elizabetha meningitidis and pseudomonas putida, and can be used as the aquatic bacterium bacteriostatic agentfor aquaculture.

The invention provides the DNA sequences of a beta-carotene hydroxylase gene (crtZ) and a 2,2'-betahydroxylase gene (crtG) in sphingomonas sp., recombinant strains lacking beta-carotene hydroxylase and 2,2'-betahydroxylase and the use of the two strains. The nucleotide sequence of the crtZ gene is represented by SEQ ID NO.1; and the nucleotide sequence of the crtG gene is represented by SEQ ID NO.2. The DNA sequences of the beta-carotene hydroxylase gene and the 2,2'-betahydroxylase gene in the sphingomonas sp., which are provided by the invention, lay a foundation for the genetic modification of a sphingomonas sp. carotenoid biological synthesis means. In addition, compared with wild strains, the recombinant strains of the sphingomonas sp., which are constructed by a gene knockout method, have the advantages that: gellengum yield is basically unchanged; and beta-carotene or zeaxanthin can be produced in the production of gellengum by fermentation. And the strains can be used in further gene knockout or metabolic engineering modification.

The invention relates to application of Chinese herbal medicament to delaying of bacterial drug resistance. The Chinese herbal medicament comprises isatis roots, blackberry lily, radix sophorae flavescentis, folium isatidis, plantain herbs, fructus forsythiae, scutellaria baicalensis or wormwood. The Chinese herbal medicament has the advantage that the traditional Chinese medicine which is selected out has the effect of delaying aeromonas drug resistance.

The invention discloses a medicine for preventing and treating crucian carp enteritis. A medicine composition is prepared from the following ingredients in parts by weight: 20 to 30 parts of fructus chebulae, 15 to 30 parts of cortex phellodendri, 15 to 30 parts of rhizoma atractylodis, 20 to 30 parts of radix scutellariae, 5 to 10 parts of rhizoma coptidis, 7 to 8 parts of herba portulacae, 8 to 14 parts of radix aucklandiae, 10 to 15 parts of radix arnebiae seu lithospermi, 3 to 5 parts of radix rubiae, 5 to 8 parts of pomegranate bark, 20 to 30 parts of manyflower glorybower roots, 5 to 7 parts of cortex lycii, 20 to 30 parts of honeysuckle flowers, 17 to 28 parts of fructus forsythiae, 15 to 35 parts of wild chrysanthemum flowers, 18 to 32 parts of radix sanguisorbae and 8 to 15 parts of plant soot. By using the medicine in compatibility; the activity of aeromonas punctata f. intestinalis can be effectively inhibited; pathogenic bacteria and bacteria in the body can be effectively killed after the medicine is taken for many times; after the medicine composition is used, both the prevention effect and the treatment effect are achieved; the curative effect taking speed is high; the medicine is harmless to fish bodies; no medicine residue exists; no harm is caused on the human bodies after the medicine is taken; and meanwhile, the immunity of the fish bodies can be enhanced.

The invention discloses a medicine for treating white spot disease of turtle. The medicine consists of gentian violet, fructus forsythiae, blackberry lily, sodium chloride, houttuynia cordata, honeysuckle, amur cork tree bark and common cnidium fruit. When resisting fungi, the medicine disclosed by the invention has a certain capacity of inhibiting aeromonas, so that the medicine can be used for treating white spot disease of turtle. Through reasonable combination, dried orange peel, hawthorn, houttuynia cordata, honeysuckle, wild chrysanthemum, amur cork tree bark and the like can eliminate the heavy flavor of garlic when resisting the fungi. Liquorice root can be used for protecting liver so as to protect liver cells from being hurt by tannin of gallnut and the like. Through a preparation method, an active ingredient extraction ratio is improved and the loss of beneficial materials is reduced. The garlic is mixed with the dried orange peel, so that the flavor of the garlic is eliminated in advance.

The invention provides a carbon source used for regulation and control of microbial community in culture pond water body and an application, the carbon source comprises the following components by weight part: 60-100 parts of soluble starch, 5-20 parts of vegetable protein and 5-20 parts of micromolecule organic acidity sodium salt. The experiment proves that the compound carbon source is capable of reducing ammonia nitrogen concentration of the pond water body, increasing algae abundance and controlling the aeromonas quantity, accordingly, the carbon source has large application potential on regulation and control of microbial community in culture pond water body.

An antibacterial medicinef or aquatic animals, such as mandarinfish, is prepared from uritrate, VC and starch.

Aeromonas hydrophila is a reemerging pathogen of channel catfish (Ictalurus punctatus); recent outbreaks from 2009 to 2014 have caused the loss of more than 12 million pounds of market size catfish in Alabama and Mississippi. Genome sequencing revealed a clonal group of A. hydrophila isolates with unique genetic and phenotypic features that is highly pathogenic in channel catfish. Comparison of the genome sequence of a representative catfish isolate (ML09-119) from this virulent clonal group with lower virulence A. hydrophila isolates revealed four fimbrial proteins unique to strain ML09-119. In this work, we expressed and purified four A. hydrophila fimbrial proteins (FimA, Fim, MrfG, and FimOM) and assessed their ability to protect and stimulate protective immunity in channel catfish fingerlings against A. hydrophila ML09-119 infection for vaccine development. Our results showed catfish immunized with FimA, Fim, FimMrfG, and FimOM exhibited 59.83%, 95.41%, 85.72%, and 75.01% relative percent survival, respectively, after challenge with A. hydrophila strain ML09-119. Bacterial concentrations in liver, spleen, and anterior kidney were significantly (p<0.05) lower in vaccinated fish compared to the non-vaccinated sham groups at 48 h post-infection. However, only the Fim immunized group showed a significantly higher antibody titer in comparison to the non-vaccinated treatment group (p<0.05) at 21 days post-vaccination. Altogether, Fim and FimMrfG recombinant proteins have potential for vaccine development against virulent A. hydrophila infection. Genomic subtraction revealed three outer membrane proteins present in strain ML09-119 but not in the low virulence reference A. hydrophila strain; the major outer membrane protein OmpAI (OmpA1), TonB-dependent receptor (TonB-DR), and transferrin-binding protein A (TbpA). Here, the genes encoding OmpAI, tonB-DR, and tbpA were cloned from A. hydrophila ML09-119 and were expressed into Escherichia coli. The purified recombinant OmpA, TonB-DR, and TbpA proteins had estimated molecular weights of 37.26, 78.55, and 41.67 kDa, respectively. Catfish fingerlings vaccinated with OmpA1, TonB-DR, and TbpA emulsified with non-mineral oil adjuvant were protected against the subsequent A. hydrophila ML09-119 infection with 98.59%, 95.59%, and 47.89% relative percent survival (RPS), respectively. Furthermore, the mean liver, spleen, and anterior kidney bacterial loads were significantly lower in catfish vaccinated with the OmpA1 and TonB-DR than the non-vaccinated control group. ELISA demonstrated that catfish immunized with OmpA1, TonB-DR, and TbpA produce significant antibody response by 21 days post-immunization. Therefore, data generated during the study suggest that OmpAI and TonB-DR proteins could be used as potential candidates for vaccine development against A. hydrophila epidemic strain infection. However, TbpA protein failed to provide such strong protection. Recombinant ATPase from A. hydrophila also showed promise as a vaccine antigen. A live attenuated vaccine was prepared that combined the advantages of a live attenuated vaccine (ESC-NDKL1 (ΔgcvPΔsdhCΔfrdA) mutant of Edwardsiella ictaluri) against enteric septicemia of catfish (ESC) and three immunogenic recombinant proteins (Fim, FimMrfg, and ATPase) against A. hydrophila infection. Our results showed channel catfish fingerlings immersion-vaccinated with ESC-NDKL1::pETfim, ESC-NDKL1::pETmrfG, and ESC-NDKL1::pETATPase exhibited 100%, 91.67%, and 100% percent survival after challenge with the A. hydrophila ML09-119, which was significantly less than non-vaccinated group (88.89% mortality). In a second study, Catfish immunized with NDKL1::pETfim, ESC-NDKL1::pETmrfG, ESC-NDKL1::pETATPase had significantly (p<0.05) lower mortalities than sham-vaccinated group.

A light green balloon bacteriophage AVP is brought forward, deposited in China typical culture preservation center with that deposit number of CCTCC NO:M 2018155, deposited on 25 March 2018. The new light green balloon bacteriophage AVP can lyse the bacteriophage AVP lysate of Aeromonas aeruginosa, the bacteriophage has a wide lysis spectrum, not only can it lyse the pale green balloon bacteria, It can also lyse Staphylococcus aureus and Staphylococcus epidermidis. It can lyse standard strains or clinical strains isolated from environment or diseased animal feces. It can be used alone or in combination with other substances to provide a safe and non-toxic bacteriophage sterilization product for disinfection and purification of environment.

The invention discloses primers capable of simultaneously detecting various diarrhea pathogenic bacteria and an application of the primers, and belongs to the field of nucleic acid detection of pathogenic bacteria. The invention discloses primers and probes as shown in SEQ ID NO: 1-39; and the primers and the probes can be used for rapidly and accurately detecting staphylococcus aureus, shigella, vibrio parahaemolyticus, yersinia enterocolitica, clostridium difficile, plesiomonas sp, listeria monocytogenes, escherichia coli O157:H7, aeromonas, salmonella, clostridium perfringens, bacillus cereus and campylobacter jejuni; therefore, great convenience is brought to clinical microorganism detection.

The invention discloses a medicine for treating white-spot disease caused by aeromonas. The medicine comprises fructus forsythiae, licorice root, garlic, Chinese gall, radix isatidis, rheum officinale, lonicera japonica, chrysanthemum indicum, ash bark, herba houttuyniae, Japanese buttercup herb, coptis chinensis, radix scutellariae, cortex phellodendri, pericarpium citri reticulatae, medicine terminalia fruit, sweet wormwood, rhizoma belamcandae, alisma plantago-aquatica linn, angelica sinensis, asparagus fern, fructus mume, pangolin, flos paulowniae, Japanese cayratia herb, solanum lyratum thunb and radices trichosanthis. The traditional Chinese medicine can be used for treating white-spot disease with a remarkable curative effect, and the curative rate of 30 days of the traditional Chinese medicine can be 90 percent. Garlic and other materials having spicy flavor are used, but other medicinal materials are reasonably combined, so that the aim of removing smell without removing curative effects can be achieved, and the curative effect can be further improved when the medicine is especially combined with western medicine antibiotics and added with calcined gypsum and other components.

The invention discloses an egg yolk antibody microcapsule feed additive for resisting carassius auratus gibelio bacterial septicemia. The additive consists of three kinds of raw materials, namely, egg yolk antibody freeze-dried powder for resisting the carassius auratus gibelio bacterial septicemia, astragalus powder and beta-cyclodextrin, wherein the egg yolk antibody freeze-dried powder for resisting the carassius auratus gibelio bacterial septicemia is mainly used for lowering the infection rate of carassius auratus gibelio by three kinds of mesophilic aeromonas or preventing carassius auratus gibelio from being infected by the three kinds of mesophilic aeromonas; the astragalus powder is used for enhancing the immunity of the carassius auratus gibelio; the beta-cyclodextrin is mainly used for realizing embedding and slow release of the egg yolk antibody freeze-dried powder. When the microcapsule feed additive and a commercially available carassius auratus gibelio compound feed are fed together according to a certain proportion, diseases caused by three kinds of pathogenic mesophilic aeromonas can be prevented and treated during cultivation of the carassius auratus gibelio, the yield of the carassius auratus gibelio is increased, and the economical benefit of carassius auratus gibelio cultivation is increased remarkably. The problem of bacterial septicemia in current carassius auratus gibelio production is solved.